Pxn

Summary

Gene Symbol: Pxn
Description: paxillin
Alias: AW108311, AW123232, Pax, paxillin
Species: mouse
Products:     Pxn

Top Publications

  1. Robertson L, Mireau L, Ostergaard H. A role for phosphatidylinositol 3-kinase in TCR-stimulated ERK activation leading to paxillin phosphorylation and CTL degranulation. J Immunol. 2005;175:8138-45 pubmed
    ..Furthermore, we identified the cytoskeletal adaptor paxillin as a target of ERK downstream of TCR stimulation...
  2. Lewis J, Schwartz M. Integrins regulate the association and phosphorylation of paxillin by c-Abl. J Biol Chem. 1998;273:14225-30 pubmed
    ..transiently co-precipitate with c-Abl during cell adhesion, and one was identified as the focal adhesion protein paxillin. Abl also became transiently phosphorylated in response to cell adhesion...
  3. Robertson L, Ostergaard H. Paxillin associates with the microtubule cytoskeleton and the immunological synapse of CTL through its leucine-aspartic acid domains and contributes to microtubule organizing center reorientation. J Immunol. 2011;187:5824-33 pubmed publisher
    The cytoskeletal adaptor protein paxillin localizes to the microtubule organizing center (MTOC) in T cells and, upon target cell binding, is recruited to the supramolecular activation complex (SMAC)...
  4. Brown M, Cary L, Jamieson J, Cooper J, Turner C. Src and FAK kinases cooperate to phosphorylate paxillin kinase linker, stimulate its focal adhesion localization, and regulate cell spreading and protrusiveness. Mol Biol Cell. 2005;16:4316-28 pubmed
    The ArfGAP paxillin kinase linker (PKL)/G protein-coupled receptor kinase-interacting protein (GIT)2 has been implicated in regulating cell spreading and motility through its transient recruitment of the p21-activated kinase (PAK) to ..
  5. Liu Z, Yu C, Nickel C, Thomas S, Cantley L. Hepatocyte growth factor induces ERK-dependent paxillin phosphorylation and regulates paxillin-focal adhesion kinase association. J Biol Chem. 2002;277:10452-8 pubmed
    ..epithelial cells, we studied the effect of HGF on the focal adhesion proteins, focal adhesion kinase (FAK) and paxillin and their association...
  6. Ballestrem C, Erez N, Kirchner J, Kam Z, Bershadsky A, Geiger B. Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer. J Cell Sci. 2006;119:866-75 pubmed
    ..Here we studied molecular interactions and tyrosine phosphorylation of paxillin, Crk-associated substrate (CAS), and focal adhesion kinase (FAK) in focal adhesions...
  7. Nishiya N, Shirai T, Suzuki W, Nose K. Hic-5 interacts with GIT1 with a different binding mode from paxillin. J Biochem. 2002;132:279-89 pubmed
    Hic-5, a member of the paxillin family of adaptor molecules, is localized at focal adhesion and implicated in integrin-mediated signaling...
  8. Chay K, Park S, Mushinski J. Linkage of caspase-mediated degradation of paxillin to apoptosis in Ba/F3 murine pro-B lymphocytes. J Biol Chem. 2002;277:14521-9 pubmed
    We have cloned the complete cDNA from mouse paxillin, a 68-kDa adapter protein found in focal adhesions...
  9. Wade R, Bohl J, Vande Pol S. Paxillin null embryonic stem cells are impaired in cell spreading and tyrosine phosphorylation of focal adhesion kinase. Oncogene. 2002;21:96-107 pubmed
    b>Paxillin is a focal-adhesion associated protein implicated in the regulation of integrin signaling and organization of the actin cytoskeleton...

More Information

Publications86

  1. Vatsa A, Semeins C, Smit T, Klein Nulend J. Paxillin localisation in osteocytes--is it determined by the direction of loading?. Biochem Biophys Res Commun. 2008;377:1019-24 pubmed publisher
    ..mechanical loading of cells aligns cytoskeletal stress fibres in the direction of principle strains and localises paxillin to the mechanosensing region...
  2. Ishibe S, Joly D, Liu Z, Cantley L. Paxillin serves as an ERK-regulated scaffold for coordinating FAK and Rac activation in epithelial morphogenesis. Mol Cell. 2004;16:257-67 pubmed
    ..In the present study we demonstrate that activated ERK phosphorylates paxillin on serine 83 and that mutation of this site eliminates HGF-stimulated increased association of paxillin and FAK ..
  3. Kanchanawong P, Shtengel G, Pasapera A, Ramko E, Davidson M, Hess H, et al. Nanoscale architecture of integrin-based cell adhesions. Nature. 2010;468:580-4 pubmed publisher
    ..a membrane-apposed integrin signalling layer containing integrin cytoplasmic tails, focal adhesion kinase and paxillin; an intermediate force-transduction layer containing talin and vinculin; and an uppermost actin-regulatory layer ..
  4. Subauste M, Pertz O, Adamson E, Turner C, Junger S, Hahn K. Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. J Cell Biol. 2004;165:371-81 pubmed
    ..This increase is shown to result from vinculin's modulation of paxillin-FAK interactions...
  5. Raimondi C, Fantin A, Lampropoulou A, Denti L, Chikh A, Ruhrberg C. Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells. J Exp Med. 2014;211:1167-83 pubmed publisher
    ..integrin ligand fibronectin (FN) stimulated actin remodeling and phosphorylation of the focal adhesion component paxillin (PXN) in a VEGF/VEGFR2-independent but NRP1-dependent manner...
  6. Pratt S, Epple H, Ward M, Feng Y, Braga V, Longmore G. The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration. J Cell Biol. 2005;168:813-24 pubmed
  7. Jamieson J, Tumbarello D, Halle M, Brown M, Tremblay M, Turner C. Paxillin is essential for PTP-PEST-dependent regulation of cell spreading and motility: a role for paxillin kinase linker. J Cell Sci. 2005;118:5835-47 pubmed
    ..The focal adhesion adaptor protein paxillin is also necessary for normal cell migration and binds directly to PTP-PEST...
  8. Romanova L, Hashimoto S, Chay K, Blagosklonny M, Sabe H, Mushinski J. Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive. J Cell Sci. 2004;117:3759-68 pubmed
    Tyrosine phosphorylation of paxillin regulates actin cytoskeleton-dependent changes in cell morphology and motility in adherent cells...
  9. Zaidel Bar R, Milo R, Kam Z, Geiger B. A paxillin tyrosine phosphorylation switch regulates the assembly and form of cell-matrix adhesions. J Cell Sci. 2007;120:137-48 pubmed
    ..imaging, labeling with phosphospecific-antibodies and overexpression of a novel tyrosine phosphomimetic mutant of paxillin, to demonstrate that the modulation of tyrosine phosphorylation of paxillin regulates both the assembly and ..
  10. Hagel M, George E, Kim A, Tamimi R, Opitz S, Turner C, et al. The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling. Mol Cell Biol. 2002;22:901-15 pubmed
    ..We have examined the role of one of the best-characterized integrin effectors, the focal adhesion protein paxillin, by disruption of the paxillin gene in mice...
  11. Pasapera A, Schneider I, Rericha E, Schlaepfer D, Waterman C. Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation. J Cell Biol. 2010;188:877-90 pubmed publisher
    ..Myosin II activity promotes FAK/Src-mediated phosphorylation of paxillin on tyrosines 31 and 118 and vinculin association with paxillin...
  12. Deakin N, Ballestrem C, Turner C. Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA. PLoS ONE. 2012;7:e37990 pubmed publisher
    ..FRAP and biochemical analyses we show that the interactions between the focal adhesion proteins vinculin and paxillin, as well as the closely related family member Hic-5 are spatially and reciprocally regulated by the activity of ..
  13. Fusté N, Fernández Hernández R, Cemeli T, Mirantes C, Pedraza N, Rafel M, et al. Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin. Nat Commun. 2016;7:11581 pubmed publisher
    ..Here, we show that the focal adhesion component paxillin is a cytoplasmic substrate of Ccnd1·Cdk4...
  14. Digman M, Wiseman P, Choi C, Horwitz A, Gratton E. Stoichiometry of molecular complexes at adhesions in living cells. Proc Natl Acad Sci U S A. 2009;106:2170-5 pubmed publisher
    ..We apply the cross-variance method to determine the stoichiometry of complexes containing paxillin and vinculin or focal adhesion kinase (FAK) in disassembling adhesions in mouse embryo fibroblasts expressing FAK,..
  15. Wu D, Wu T, Wu J, Cheng Y, Chen Y, Lee M, et al. Phosphorylation of paxillin confers cisplatin resistance in non-small cell lung cancer via activating ERK-mediated Bcl-2 expression. Oncogene. 2014;33:4385-95 pubmed publisher
    b>Paxillin (PXN) is required for receptor tyrosine kinase-mediated ERK activation, and the activation of the Raf/MEK/ERK cascade has been linked with Bcl-2 expression...
  16. Sharifi M, Mowers E, Drake L, Collier C, Chen H, Zamora M, et al. Autophagy Promotes Focal Adhesion Disassembly and Cell Motility of Metastatic Tumor Cells through the Direct Interaction of Paxillin with LC3. Cell Rep. 2016;15:1660-72 pubmed publisher
    ..tumor cells, reflecting a role for autophagy in FA disassembly through targeted degradation of paxillin. We demonstrate that paxillin interacts with processed LC3 through a conserved LIR motif in the amino-terminal ..
  17. Schindeler A, Lavulo L, Harvey R. Muscle costameric protein, Chisel/Smpx, associates with focal adhesion complexes and modulates cell spreading in vitro via a Rac1/p38 pathway. Exp Cell Res. 2005;307:367-80 pubmed
    ..Csl co-localized with actin networks at peripheral membranes, and with focal adhesion proteins vinculin, paxillin, integrin beta1, and the small GTPase Rac1...
  18. Kessels M, Engqvist Goldstein A, Drubin D. Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation. Mol Biol Cell. 2000;11:393-412 pubmed
    ..mAbp1 is a newly identified cytoskeletal protein in mice and may serve as a signal-responsive link between the dynamic cortical actin cytoskeleton and regions of membrane dynamics. ..
  19. Zouq N, Keeble J, Lindsay J, Valentijn A, Zhang L, Mills D, et al. FAK engages multiple pathways to maintain survival of fibroblasts and epithelia: differential roles for paxillin and p130Cas. J Cell Sci. 2009;122:357-67 pubmed publisher
    ..However FAK regulates survival through a more restricted set of pathways in the more anoikis-sensitive epithelial cells. Furthermore, we identify a novel role for paxillin in apoptosis suppression.
  20. Woodrow M, Woods D, Cherwinski H, Stokoe D, McMahon M. Ras-induced serine phosphorylation of the focal adhesion protein paxillin is mediated by the Raf-->MEK-->ERK pathway. Exp Cell Res. 2003;287:325-38 pubmed
    ..We identified Raf-induced serine phosphorylation of the adaptor protein paxillin in a variety of cell types...
  21. Mazaki Y, Uchida H, Hino O, Hashimoto S, Sabe H. Paxillin isoforms in mouse. Lack of the gamma isoform and developmentally specific beta isoform expression. J Biol Chem. 1998;273:22435-41 pubmed
    b>Paxillin, a focal adhesion protein, exists as multiple isoforms in humans (alpha, beta, and gamma). To understand more about the physiological role of each isoform, we have employed the mouse system...
  22. Du T, Qu Y, Li J, Li H, Su L, Zhou Q, et al. Maternal embryonic leucine zipper kinase enhances gastric cancer progression via the FAK/Paxillin pathway. Mol Cancer. 2014;13:100 pubmed publisher
    ..molecular mechanism and effect of MELK on migration and invasion, Western blotting was used to analyze the FAK/Paxillin pathway and pull down assays for the activity of small Rho GTPases...
  23. Jacinto E, Loewith R, Schmidt A, Lin S, Ruegg M, Hall A, et al. Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol. 2004;6:1122-8 pubmed
    ..mTORC2 is not upstream of the mTORC1 effector S6K. Thus, two distinct TOR complexes constitute a primordial signalling network conserved in eukaryotic evolution to control the fundamental process of cell growth. ..
  24. Louvi A, Nishimura S, Gunel M. Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration. Development. 2014;141:1404-15 pubmed publisher
    ..Thus, we identify a novel cytoplasmic regulator of neuronal migration and demonstrate that its inactivation in radial glia progenitors and nascent neurons produces severe malformations of cortical development. ..
  25. Thomas R, Schmedt C, Novelli M, Choi B, Skok J, Tarakhovsky A, et al. C-terminal SRC kinase controls acute inflammation and granulocyte adhesion. Immunity. 2004;20:181-91 pubmed
    ..Hyperphosphorylation of key signaling proteins such as Syk and Paxillin in mutant granulocytes further supported breakdown of the activation threshold set by Csk...
  26. Lv Z, Hu M, Ren X, Fan M, Zhen J, Chen L, et al. Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro. J Diabetes Res. 2016;2016:5671803 pubmed publisher
    ..In this study, we investigated the potential role of Fyn/ROCK signaling and its interactions with paxillin. Our results presented that high glucose led to filamentous actin (F-actin) rearrangement in podocytes, ..
  27. Jacob A, Amack J, Turner C. Paxillin genes and actomyosin contractility regulate myotome morphogenesis in zebrafish. Dev Biol. 2017;425:70-84 pubmed publisher
    b>Paxillin (Pxn) is a key adapter protein and signaling regulator at sites of cell-extracellular matrix (ECM) adhesion. Here, we investigated the role of Pxn during vertebrate development using the zebrafish embryo as a model system...
  28. Lallemand D, Curto M, Saotome I, Giovannini M, McClatchey A. NF2 deficiency promotes tumorigenesis and metastasis by destabilizing adherens junctions. Genes Dev. 2003;17:1090-100 pubmed
    ..Our studies indicate that merlin functions as a tumor and metastasis suppressor by controlling cadherin-mediated cell:cell contact. ..
  29. Schell C, Rogg M, Suhm M, Helmstädter M, Sellung D, Yasuda Yamahara M, et al. The FERM protein EPB41L5 regulates actomyosin contractility and focal adhesion formation to maintain the kidney filtration barrier. Proc Natl Acad Sci U S A. 2017;114:E4621-E4630 pubmed publisher
  30. Miyamoto Y, Torii T, Yamamori N, Eguchi T, Nagao M, Nakamura K, et al. Paxillin is the target of c-Jun N-terminal kinase in Schwann cells and regulates migration. Cell Signal. 2012;24:2061-9 pubmed publisher
    ..Here we show that JNK, acting through phosphorylation of the cytoskeletal protein paxillin, regulates Schwann cell migration and that it mediates dorsal root ganglion (DRG) neuronal conditioned medium (CM)..
  31. Tanaka T, Moriwaki K, Murata S, Miyasaka M. LIM domain-containing adaptor, leupaxin, localizes in focal adhesion and suppresses the integrin-induced tyrosine phosphorylation of paxillin. Cancer Sci. 2010;101:363-8 pubmed publisher
    Focal adhesion (FA) consists of multiple cellular proteins including paxillin and serves as a center for adhesion-mediated signaling...
  32. Barry D, Xu K, Meadows S, Zheng Y, Norden P, Davis G, et al. Cdc42 is required for cytoskeletal support of endothelial cell adhesion during blood vessel formation in mice. Development. 2015;142:3058-70 pubmed publisher
    ..This work supports the notion of Cdc42 as a central regulator of the cellular machinery in ECs that drives blood vessel formation. ..
  33. Bach C, Creed S, Zhong J, Mahmassani M, Schevzov G, Stehn J, et al. Tropomyosin isoform expression regulates the transition of adhesions to determine cell speed and direction. Mol Cell Biol. 2009;29:1506-14 pubmed publisher
    ..Moreover, our data suggest that the expression of Tm5NM1 is a critical determinant of paxillin phosphorylation, a signaling event that is necessary for focal adhesion disassembly...
  34. Srinivasan R, Zabuawala T, Huang H, Zhang J, Gulati P, Fernandez S, et al. Erk1 and Erk2 regulate endothelial cell proliferation and migration during mouse embryonic angiogenesis. PLoS ONE. 2009;4:e8283 pubmed publisher
    ..Interestingly, both Paxillin and Focal Adhesion Kinase were expressed at lower levels in endothelial cells lacking Erk1 and Erk2 both in vivo ..
  35. Cariaga Martínez A, Cortés I, García E, Pérez García V, Pajares M, Idoate M, et al. Phosphoinositide 3-kinase p85beta regulates invadopodium formation. Biol Open. 2014;3:924-36 pubmed publisher
    ..These results show that p85β enhances invasion by inducing cell adhesion development into invadopodia-like structures explaining the metastatic potential of tumors with increased p85β levels. ..
  36. Dobkin Bekman M, Naidich M, Rahamim L, Przedecki F, Almog T, Lim S, et al. A preformed signaling complex mediates GnRH-activated ERK phosphorylation of paxillin and FAK at focal adhesions in L beta T2 gonadotrope cells. Mol Endocrinol. 2009;23:1850-64 pubmed publisher
    ..protein kinase C delta, -epsilon, and -alpha, Ras, MAPK kinase 1/2, ERK1/2, tubulin, focal adhesion kinase (FAK), paxillin, vinculin, caveolin-1, kinase suppressor of Ras-1, and the GnRHR...
  37. Kopecki Z, O Neill G, Arkell R, Cowin A. Regulation of focal adhesions by flightless i involves inhibition of paxillin phosphorylation via a Rac1-dependent pathway. J Invest Dermatol. 2011;131:1450-9 pubmed publisher
    ..that a functional effect of Flii at both the cellular level and in in vivo mouse wounds is through inhibiting paxillin tyrosine phosphorylation and suppression of signaling proteins Src and p130Cas, both of which regulate adhesion ..
  38. Vindis C, Teli T, Cerretti D, Turner C, Huynh Do U. EphB1-mediated cell migration requires the phosphorylation of paxillin at Tyr-31/Tyr-118. J Biol Chem. 2004;279:27965-70 pubmed
    ..Here we demonstrate that activated EphB1 induces tyrosine phosphorylation of the focal adhesion protein paxillin at Tyr-31 and Tyr-118 and is recruited to paxillin-focal adhesion kinase (FAK) complexes...
  39. Böttcher R, Veelders M, Rombaut P, Faix J, Theodosiou M, Stradal T, et al. Kindlin-2 recruits paxillin and Arp2/3 to promote membrane protrusions during initial cell spreading. J Cell Biol. 2017;216:3785-3798 pubmed publisher
    ..kindlin-2 plays a central role for cell adhesion and membrane protrusion by directly binding and recruiting paxillin to nascent adhesions...
  40. van der Flier A, Kuikman I, Kramer D, Geerts D, Kreft M, Takafuta T, et al. Different splice variants of filamin-B affect myogenesis, subcellular distribution, and determine binding to integrin [beta] subunits. J Cell Biol. 2002;156:361-76 pubmed
    ..These findings suggest that specific combinations of filamin mRNA splicing events modulate the organization of the actin cytoskeleton and the binding affinity for integrins. ..
  41. Neet K, Hunter T. The nonreceptor protein-tyrosine kinase CSK complexes directly with the GTPase-activating protein-associated p62 protein in cells expressing v-Src or activated c-Src. Mol Cell Biol. 1995;15:4908-20 pubmed
    ..Our results suggest that GAP-A.p62 may function as a docking protein and may mediate translocation of proteins, including GAP and CSK, to membrane or cytoskeletal regions upon c-Src activation. ..
  42. Romanova L, Holmes G, Bahte S, Kovalchuk A, Nelson P, Ward Y, et al. Phosphorylation of paxillin at threonine 538 by PKCdelta regulates LFA1-mediated adhesion of lymphoid cells. J Cell Sci. 2010;123:1567-77 pubmed publisher
    We investigated the PKCdelta-mediated phosphorylation of paxillin within its LIM4 domain and the involvement of this phosphorylation in activation of LFA-1 integrins of the Baf3 pro-B lymphocytic cell line...
  43. Yamauchi J, Miyamoto Y, Sanbe A, Tanoue A. JNK phosphorylation of paxillin, acting through the Rac1 and Cdc42 signaling cascade, mediates neurite extension in N1E-115 cells. Exp Cell Res. 2006;312:2954-61 pubmed
    ..Additionally, paxillin, the multifunctional focal adhesion protein, is phosphorylated at Ser 178 by upregulation of the Rac1/Cdc42/JNK ..
  44. Kleveta G, Borzęcka K, Zdioruk M, Czerkies M, Kuberczyk H, Sybirna N, et al. LPS induces phosphorylation of actin-regulatory proteins leading to actin reassembly and macrophage motility. J Cell Biochem. 2012;113:80-92 pubmed publisher
    ..Simultaneously with actin polymerization, LPS stimulated phosphorylation of two actin-regulatory proteins, paxillin on tyrosine 118 by 80% and N-WASP on serine 484/485 by 20%, and these events preceded activation of NF-?B...
  45. Phillips H, Papoutsi T, Soenen H, Ybot Gonzalez P, Henderson D, Chaudhry B. Neural crest cell survival is dependent on Rho kinase and is required for development of the mid face in mouse embryos. PLoS ONE. 2012;7:e37685 pubmed publisher
    ..We propose that reduced NCC numbers in the frontonasal processes and first pharyngeal arch, resulting from exacerbated cell death, may be the common mechanism underlying frontonasal dysplasia. ..
  46. Bianchi Smiraglia A, Kunnev D, Limoge M, Lee A, Beckerle M, Bakin A. Integrin-?5 and zyxin mediate formation of ventral stress fibers in response to transforming growth factor ?. Cell Cycle. 2013;12:3377-89 pubmed publisher
    ..These findings provide a platform for defining the molecular mechanism regulating the organization and activities of VSFs in response to TGF-?. ..
  47. Zhao Y, Zhang X, Guda K, Lawrence E, Sun Q, Watanabe T, et al. Identification and functional characterization of paxillin as a target of protein tyrosine phosphatase receptor T. Proc Natl Acad Sci U S A. 2010;107:2592-7 pubmed publisher
    ..However, the cell signaling pathways regulated by PTPRT largely remain to be elucidated. Here, we show that paxillin is a direct substrate of PTPRT and that PTPRT specifically regulates paxillin phosphorylation at tyrosine residue ..
  48. Bechara A, Nawabi H, Moret F, Yaron A, Weaver E, Bozon M, et al. FAK-MAPK-dependent adhesion disassembly downstream of L1 contributes to semaphorin3A-induced collapse. EMBO J. 2008;27:1549-62 pubmed publisher
    ..This reveals that specific signalling activities downstream of L1 and Plexin-As cooperate for mediating the axon guidance effects of Sema3A. ..
  49. Roy S, Ruest P, Hanks S. FAK regulates tyrosine phosphorylation of CAS, paxillin, and PYK2 in cells expressing v-Src, but is not a critical determinant of v-Src transformation. J Cell Biochem. 2002;84:377-88 pubmed
    ..that also plays a role in recruiting v-Src to phosphorylate substrates CAS (Crk-associated substrate) and paxillin. The FAK Tyr-397 autophosphorylation site was necessary for this scaffolding function, but was not required for v-..
  50. Badowski C, Pawlak G, Grichine A, Chabadel A, Oddou C, Jurdic P, et al. Paxillin phosphorylation controls invadopodia/podosomes spatiotemporal organization. Mol Biol Cell. 2008;19:633-45 pubmed
    ..We have shown that invadopodia ring expansion is controlled by paxillin phosphorylations on tyrosine 31 and 118, which allows invadopodia disassembly...
  51. Jackson J, Young M. Protein phosphatase-2A modulates the serine and tyrosine phosphorylation of paxillin in Lewis lung carcinoma tumor variants. Clin Exp Metastasis. 2002;19:409-15 pubmed
    ..Focal adhesion dynamics are, in turn, influenced by the serine and tyrosine phosphorylation state of paxillin. Using Lewis lung carcinoma (LLC) tumor variants, this study shows the importance of the serine/threonine protein ..
  52. Sundd P, Gutierrez E, Petrich B, Ginsberg M, Groisman A, Ley K. Live cell imaging of paxillin in rolling neutrophils by dual-color quantitative dynamic footprinting. Microcirculation. 2011;18:361-72 pubmed publisher
    ..To assess the localization of the cytoskeletal protein paxillin, we applied DqDF to DiO-stained neutrophils of mice expressing an mCherry-paxillin fusion protein...
  53. Sanger J, Wang J, Gleason L, Chowrashi P, Dube D, Mittal B, et al. Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules. Cytoskeleton (Hoboken). 2010;67:808-23 pubmed publisher
    ..Our results indicate that ArgBP2 may play an important role in the assembly and maintenance of myofibrils in cardiomyocytes. ..
  54. Lim S, Chen X, Tomar A, Miller N, Yoo J, Schlaepfer D. Knock-in mutation reveals an essential role for focal adhesion kinase activity in blood vessel morphogenesis and cell motility-polarity but not cell proliferation. J Biol Chem. 2010;285:21526-36 pubmed publisher
    ..This work supports the use of FAK inhibitors to disrupt neovascularization. ..
  55. Walsh J, Young M. TGF-beta regulation of focal adhesion proteins and motility of premalignant oral lesions via protein phosphatase 1. Anticancer Res. 2011;31:3159-64 pubmed
    ..The present study focused on TGF-?-modulation of paxillin and the serine/threonine protein phosphatase PP-1, and the impact on cellular motility...
  56. Yamauchi J, Miyamoto Y, Kusakawa S, Torii T, Mizutani R, Sanbe A, et al. Neurofibromatosis 2 tumor suppressor, the gene induced by valproic acid, mediates neurite outgrowth through interaction with paxillin. Exp Cell Res. 2008;314:2279-88 pubmed publisher
    ..We previously demonstrated that c-Jun N-terminal kinase (JNK) phosphorylation of the focal adhesion protein paxillin mediates differentiation in N1E-115 neuroblastoma cells...
  57. Deakin N, Turner C. Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration. J Cell Biol. 2014;206:395-413 pubmed publisher
    ..In this paper, we have identified a new and conserved role for the focal adhesion scaffold protein paxillin in regulating the posttranslational modification of the MT cytoskeleton through an inhibitory interaction with ..
  58. Carlisle F, Steel K, Lewis M. Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti. Gene Expr Patterns. 2012;12:172-9 pubmed publisher
    ..Odf2, as its centriolar isoform Cenexin, marks the dendrites extending to and contacting hair cells, and Pxn, a focal adhesion scaffold protein, is most strongly expressed in pillar cells during the ages studied...
  59. Engqvist Goldstein A, Kessels M, Chopra V, Hayden M, Drubin D. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles. J Cell Biol. 1999;147:1503-18 pubmed
    ..These data show that mHip1R is a component of clathrin-coated pits and vesicles and suggest that it might link the endocytic machinery to the actin cytoskeleton. ..
  60. Liu F, Sells M, Chernoff J. Transformation suppression by protein tyrosine phosphatase 1B requires a functional SH3 ligand. Mol Cell Biol. 1998;18:250-9 pubmed
    ..correlate with the phosphorylation status of p130Cas and two proteins that are associated with p130Cas, Paxillin and Fak...
  61. Kobayashi T, Hino S, Oue N, Asahara T, Zollo M, Yasui W, et al. Glycogen synthase kinase 3 and h-prune regulate cell migration by modulating focal adhesions. Mol Cell Biol. 2006;26:898-911 pubmed
    ..h-prune was localized to focal adhesions, and the siRNA for GSK-3 or h-prune delayed the disassembly of paxillin. The tyrosine phosphorylation of focal adhesion kinase (FAK) and the activation of Rac were suppressed in GSK-3 ..
  62. Woods A, Kantidakis T, Sabe H, Critchley D, Norman J. Interaction of paxillin with poly(A)-binding protein 1 and its role in focal adhesion turnover and cell migration. Mol Cell Biol. 2005;25:3763-73 pubmed
    We have previously identified poly(A)-binding protein 1 (PABP1) as a ligand for paxillin and shown that the paxillin-PABP1 complex undergoes nucleocytoplasmic shuttling...
  63. Rashid M, Belmont J, Carpenter D, Turner C, Olson E. Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation. Development. 2017;144:4002-4014 pubmed publisher
    b>Paxillin and Hic-5 are homologous focal adhesion adaptor proteins that coordinate cytoskeletal rearrangements in response to integrin signaling, but their role(s) in cortical development are unknown...
  64. Luxenburg C, Winograd Katz S, Addadi L, Geiger B. Involvement of actin polymerization in podosome dynamics. J Cell Sci. 2012;125:1666-72 pubmed publisher
    ..here that the earliest visible step in podosome assembly is the local accumulation of the plaque protein paxillin, along with cortactin, which stabilizes actin networks, followed by robust polymerization of actin filaments and ..
  65. Abshire M, Thomas K, Owen K, Bouton A. Macrophage motility requires distinct α5β1/FAK and α4β1/paxillin signaling events. J Leukoc Biol. 2011;89:251-7 pubmed publisher
    ..The scaffolding molecule paxillin functions independently of FAK to promote chemotaxis downstream of α4β1...
  66. Moeller M, Shi Y, Reichardt L, Ethell I. EphB receptors regulate dendritic spine morphogenesis through the recruitment/phosphorylation of focal adhesion kinase and RhoA activation. J Biol Chem. 2006;281:1587-98 pubmed
    ..through assembly of an EphB-associated protein complex that includes focal adhesion kinase (FAK), Src, Grb2, and paxillin and the subsequent activations of FAK, Src, paxillin, and RhoA...
  67. Ishibe S, Joly D, Zhu X, Cantley L. Phosphorylation-dependent paxillin-ERK association mediates hepatocyte growth factor-stimulated epithelial morphogenesis. Mol Cell. 2003;12:1275-85 pubmed
    ..by the tyrosine phosphorylation-dependent association of inactive ERK and the focal adhesion complex protein paxillin. In addition, paxillin was found to associate with the upstream MAP kinases Raf and MEK, resulting in a complex ..
  68. Deramaudt T, Dujardin D, Noulet F, Martin S, Vauchelles R, Takeda K, et al. Altering FAK-paxillin interactions reduces adhesion, migration and invasion processes. PLoS ONE. 2014;9:e92059 pubmed publisher
    ..Using a mutagenesis approach to alter the targeting domain of FAK, we constructed a FAK mutant that fails to bind paxillin. Inhibiting FAK-paxillin interactions led to a complete loss of FAK localization at FAs together with reduced ..
  69. Lee Y, Chang A, Lin Feng M, Tsou W, Chiang I, Lai M. Paxillin phosphorylation by JNK and p38 is required for NFAT activation. Eur J Immunol. 2012;42:2165-75 pubmed publisher
    b>Paxillin is an adaptor protein associated with focal adhesion complex, and is activated by tyrosine phosphorylation through focal adhesion kinase (FAK) and Src kinase...
  70. Enciso J, Konecny C, Karpen H, Hirschi K. Endothelial cell migration during murine yolk sac vascular remodeling occurs by means of a Rac1 and FAK activation pathway in vivo. Dev Dyn. 2010;239:2570-83 pubmed publisher
    ..yolk sacs, and molecular events important for focal adhesion turnover, FAK phosphorylation (Tyr397) and FAK-paxillin association, were decreased...
  71. Rathore V, Okada M, Newman P, Newman D. Paxillin family members function as Csk-binding proteins that regulate Lyn activity in human and murine platelets. Biochem J. 2007;403:275-81 pubmed
    ..Csk is recruited to sites of SFK activity by tyrosine-phosphorylated Csk-binding proteins. Paxillin, a multidomain adaptor protein, has been shown to act as a Csk-binding protein and to inhibit Src activity during ..
  72. Takahashi Y, Miyata M, Zheng P, Imazato T, Horwitz A, Smith J. Identification of cAMP analogue inducible genes in RAW264 macrophages. Biochim Biophys Acta. 2000;1492:385-94 pubmed
    ..ABC1 was upregulated in RAW cells by cAMP, concurrent with the cAMP induction of lipid efflux to apolipoprotein A1. ..
  73. Smith M, Blankman E, Deakin N, Hoffman L, Jensen C, Turner C, et al. LIM domains target actin regulators paxillin and zyxin to sites of stress fiber strain. PLoS ONE. 2013;8:e69378 pubmed publisher
    ..Using live imaging, we show paxillin, another LIM-domain adaptor protein, is also recruited to stress fiber strain sites...
  74. Takito J, Nakamura M, Yoda M, Tohmonda T, Uchikawa S, Horiuchi K, et al. The transient appearance of zipper-like actin superstructures during the fusion of osteoclasts. J Cell Sci. 2012;125:662-72 pubmed publisher
    ..zipper-like structure, Arp3 and cortactin overlapped with the distribution of dense F-actin, whereas integrin ?3, paxillin and vinculin were localized to the periphery of the structure...
  75. Wade R, Brimer N, Lyons C, Vande Pol S. Paxillin enables attachment-independent tyrosine phosphorylation of focal adhesion kinase and transformation by RAS. J Biol Chem. 2011;286:37932-44 pubmed publisher
    b>Paxillin and HIC5 are closely related adapter proteins that regulate cell migration and are tyrosine-phosphorylated by focal adhesion kinase (FAK)...
  76. Foletta V, Moussi N, Sarmiere P, Bamburg J, Bernard O. LIM kinase 1, a key regulator of actin dynamics, is widely expressed in embryonic and adult tissues. Exp Cell Res. 2004;294:392-405 pubmed
    ..LIMK1 subcellular localization at focal adhesions in fibroblasts as revealed by co-staining with actin, paxillin and vinculin in addition to perinuclear (Golgi) and occasional nuclear localization...
  77. Heaney C, Kolibaba K, Bhat A, Oda T, Ohno S, Fanning S, et al. Direct binding of CRKL to BCR-ABL is not required for BCR-ABL transformation. Blood. 1997;89:297-306 pubmed
    ..Thus, a direct interaction of CRKL and BCR-ABL is not required for CRKL to become tyrosine phosphorylated by BCR-ABL and suggests that CRKL function may still be required for BCR-ABL function through an indirect interaction. ..