Ptk2b

Summary

Gene Symbol: Ptk2b
Description: PTK2 protein tyrosine kinase 2 beta
Alias: CADTK, CAKB, CAKbeta, E430023O05Rik, FADK2, FAK2, PYK2, Raftk, protein-tyrosine kinase 2-beta, CAK-beta, calcium-dependent tyrosine kinase, calcium-regulated non-receptor proline-rich tyrosine kinase, cell adhesion kinase beta, cellular adhesion kinase beta, focal adhesion kinase 2, proline-rich tyrosine kinase 2, related adhesion focal tyrosine kinase
Species: mouse
Products:     Ptk2b

Top Publications

  1. Le Boeuf F, Houle F, Sussman M, Huot J. Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor. Mol Biol Cell. 2006;17:3508-20 pubmed
    ..In turn, activated ROCK directly phosphorylates FAK on Ser732. Proline-rich tyrosine kinase-2 (Pyk2) is also activated in response to VEGF...
  2. Buckbinder L, Crawford D, Qi H, Ke H, Olson L, Long K, et al. Proline-rich tyrosine kinase 2 regulates osteoprogenitor cells and bone formation, and offers an anabolic treatment approach for osteoporosis. Proc Natl Acad Sci U S A. 2007;104:10619-24 pubmed
    ..Cumulative in vitro studies indicated that proline-rich tyrosine kinase 2 (PYK2) is a positive mediator of osteoclast function and activity...
  3. Boutahar N, Guignandon A, Vico L, Lafage Proust M. Mechanical strain on osteoblasts activates autophosphorylation of focal adhesion kinase and proline-rich tyrosine kinase 2 tyrosine sites involved in ERK activation. J Biol Chem. 2004;279:30588-99 pubmed
    ..CS also activated proline-rich tyrosine kinase 2 (PYK2), a tyrosine kinase highly homologous to FAK, at the 402 phosphorylation site and promoted its association to FAK ..
  4. Miyazaki T, Sanjay A, Neff L, Tanaka S, Horne W, Baron R. Src kinase activity is essential for osteoclast function. J Biol Chem. 2004;279:17660-6 pubmed
    ..Src forms a complex with Pyk2 and Cbl after adhesion-induced stimulation of alpha(V)beta(3) integrin...
  5. Kim S, Cipolla L, Guidetti G, Okigaki M, Jin J, Torti M, et al. Distinct role of Pyk2 in mediating thromboxane generation downstream of both G12/13 and integrin ?IIb?3 in platelets. J Biol Chem. 2013;288:18194-203 pubmed publisher
    Proline-rich tyrosine kinase 2 (Pyk2) is activated by various agonists in platelets. We evaluated the signaling mechanism and the functional role of Pyk2 in platelets by using pharmacological inhibitors and Pyk2-deficient platelets...
  6. Braren R, Hu H, Kim Y, Beggs H, Reichardt L, Wang R. Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation. J Cell Biol. 2006;172:151-62 pubmed
    ..This study reveals that FAK is crucial for vascular morphogenesis and the regulation of EC survival and morphology. ..
  7. Lakkakorpi P, Bett A, Lipfert L, Rodan G, Duong L. PYK2 autophosphorylation, but not kinase activity, is necessary for adhesion-induced association with c-Src, osteoclast spreading, and bone resorption. J Biol Chem. 2003;278:11502-12 pubmed
    Proline-rich tyrosine kinase 2 (PYK2) is the main adhesion-induced kinase in bone-resorbing osteoclasts...
  8. Gil Henn H, Destaing O, Sims N, Aoki K, Alles N, Neff L, et al. Defective microtubule-dependent podosome organization in osteoclasts leads to increased bone density in Pyk2(-/-) mice. J Cell Biol. 2007;178:1053-64 pubmed
    The protein tyrosine kinase Pyk2 is highly expressed in osteoclasts, where it is primarily localized in podosomes. Deletion of Pyk2 in mice leads to mild osteopetrosis due to impairment in osteoclast function...
  9. Lim Y, Lim S, Tomar A, Gardel M, Bernard Trifilo J, Chen X, et al. PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility. J Cell Biol. 2008;180:187-203 pubmed publisher
    ..Here, we show that proline-rich kinase-2 (Pyk2) levels increase upon loss of focal adhesion kinase (FAK) in mouse embryonic fibroblasts (MEFs)...

More Information

Publications87

  1. Lim S, Chen X, Lim Y, Hanson D, Vo T, Howerton K, et al. Nuclear FAK promotes cell proliferation and survival through FERM-enhanced p53 degradation. Mol Cell. 2008;29:9-22 pubmed publisher
    ..These studies define a scaffolding role for nuclear FAK in facilitating cell survival through enhanced p53 degradation under conditions of cellular stress. ..
  2. Weis S, Lim S, Lutu Fuga K, Barnes L, Chen X, Gothert J, et al. Compensatory role for Pyk2 during angiogenesis in adult mice lacking endothelial cell FAK. J Cell Biol. 2008;181:43-50 pubmed publisher
    ..Indeed, expression of the FAK-related proline-rich tyrosine kinase 2 (Pyk2) is elevated and phosphorylated in i-EC-FAK-KO blood vessels...
  3. Consonni A, Cipolla L, Guidetti G, Canobbio I, Ciraolo E, Hirsch E, et al. Role and regulation of phosphatidylinositol 3-kinase ? in platelet integrin ?2?1 signaling. Blood. 2012;119:847-56 pubmed publisher
    ..Integrin ?2?1 also caused a time-dependent stimulation of the focal kinase Pyk2 downstream of PLC?2 and intracellular Ca(2+)...
  4. Sieg D, Ilic D, Jones K, Damsky C, Hunter T, Schlaepfer D. Pyk2 and Src-family protein-tyrosine kinases compensate for the loss of FAK in fibronectin-stimulated signaling events but Pyk2 does not fully function to enhance FAK- cell migration. EMBO J. 1998;17:5933-47 pubmed
    ..We show that expression of the FAK-related PTK, Pyk2, is elevated in fibroblasts isolated from murine fak-/- embryos (FAK-) compared with cells from fak+/+ embryos (FAK+..
  5. Matsui A, Okigaki M, Amano K, Adachi Y, Jin D, Takai S, et al. Central role of calcium-dependent tyrosine kinase PYK2 in endothelial nitric oxide synthase-mediated angiogenic response and vascular function. Circulation. 2007;116:1041-51 pubmed
    The involvement of Ca2+-dependent tyrosine kinase PYK2 in the Akt/endothelial NO synthase pathway remains to be determined...
  6. Lim S, Miller N, Nam J, Chen X, Lim Y, Schlaepfer D. Pyk2 inhibition of p53 as an adaptive and intrinsic mechanism facilitating cell proliferation and survival. J Biol Chem. 2010;285:1743-53 pubmed publisher
    b>Pyk2 is a cytoplasmic tyrosine kinase related to focal adhesion kinase (FAK). Compensatory Pyk2 expression occurs upon FAK loss in mice. However, the impact of Pyk2 up-regulation remains unclear...
  7. Canobbio I, Cipolla L, Consonni A, Momi S, Guidetti G, Oliviero B, et al. Impaired thrombin-induced platelet activation and thrombus formation in mice lacking the Ca(2+)-dependent tyrosine kinase Pyk2. Blood. 2013;121:648-57 pubmed publisher
    ..study, we used a knockout murine model to analyze the contribution of the Ca(2+)-dependent focal adhesion kinase Pyk2 in platelet activation and thrombus formation in vivo...
  8. Guignandon A, Boutahar N, Rattner A, Vico L, Lafage Proust M. Cyclic strain promotes shuttling of PYK2/Hic-5 complex from focal contacts in osteoblast-like cells. Biochem Biophys Res Commun. 2006;343:407-14 pubmed
    ..strain (CS) of osteoblastic cells induced tyrosine phosphorylation of two homologous tyrosine kinases FAK and PYK2, and of two homologous adaptor proteins paxillin and Hic5, with similar kinetics...
  9. Cipolla L, Consonni A, Guidetti G, Canobbio I, Okigaki M, Falasca M, et al. The proline-rich tyrosine kinase Pyk2 regulates platelet integrin ?IIb?3 outside-in signaling. J Thromb Haemost. 2013;11:345-56 pubmed publisher
    The proline-rich tyrosine kinase Pyk2 is a focal adhesion kinase expressed in blood platelets, and is activated downstream of G-protein coupled receptors as well as integrin ?2?1...
  10. Young S, Hum J, Rodenberg E, Turner C, Pavalko F. Non-overlapping functions for Pyk2 and FAK in osteoblasts during fluid shear stress-induced mechanotransduction. PLoS ONE. 2011;6:e16026 pubmed publisher
    ..role of a second member of the FAK family of non-receptor protein tyrosine kinases, proline-rich tyrosine kinase 2 (Pyk2), and determine its role during osteoblast mechanotransduction...
  11. Okigaki M, Davis C, Falasca M, Harroch S, Felsenfeld D, Sheetz M, et al. Pyk2 regulates multiple signaling events crucial for macrophage morphology and migration. Proc Natl Acad Sci U S A. 2003;100:10740-5 pubmed
    The biological role of the protein tyrosine kinase, Pyk2, was explored by targeting the Pyk2 gene by homologous recombination. Pyk2-/- mice are viable and fertile, without overt impairment in development or behavior...
  12. Avraham S, London R, Fu Y, Ota S, Hiregowdara D, Li J, et al. Identification and characterization of a novel related adhesion focal tyrosine kinase (RAFTK) from megakaryocytes and brain. J Biol Chem. 1995;270:27742-51 pubmed
    ..We have isolated a cDNA encoding a novel human intracytoplasmic tyrosine kinase, termed RAFTK (for a related adhesion focal tyrosine kinase). In addition, we have cloned and characterized the murine homolog of the human RAFTK cDNA...
  13. Ren X, Du Q, Huang Y, Ao S, Mei L, Xiong W. Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein. J Cell Biol. 2001;152:971-84 pubmed
    Proline-rich tyrosine kinase 2 (PYK2), a tyrosine kinase structurally related to focal adhesion kinase (FAK), is implicated in regulating cytoskeletal organization...
  14. Faure C, Corvol J, Toutant M, Valjent E, Hvalby O, Jensen V, et al. Calcineurin is essential for depolarization-induced nuclear translocation and tyrosine phosphorylation of PYK2 in neurons. J Cell Sci. 2007;120:3034-44 pubmed
    Proline-rich tyrosine kinase 2 (PYK2) is a non-receptor tyrosine kinase expressed in many cell types and enriched in neurons. PYK2 is a cytoplasmic enzyme activated by increases in cytosolic free Ca(2+) through an unknown mechanism...
  15. Watanabe N, Amano N, Ishizuka H, Mashima K. Leupaxin binds to PEST domain tyrosine phosphatase PEP. Mol Cell Biochem. 2005;269:13-7 pubmed
    ..These results suggested that leupaxin might participate in the regulation of the signaling cascade through the binding to PEP in lymphocytes. ..
  16. Wang Q, Xie Y, Du Q, Wu X, Feng X, Mei L, et al. Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin. J Cell Biol. 2003;160:565-75 pubmed
    ..Proline-rich tyrosine kinase 2 (PYK2), a major cell adhesion-activated tyrosine kinase in osteoclasts, plays an important role in regulating this event...
  17. Giralt A, Coura R, Girault J. Pyk2 is essential for astrocytes mobility following brain lesion. Glia. 2016;64:620-34 pubmed publisher
    Proline-rich tyrosine kinase 2 (Pyk2) is a calcium-dependent, non-receptor protein-tyrosine kinase of the focal adhesion kinase (FAK) family. Pyk2 is enriched in the brain, especially the forebrain...
  18. Hum J, Day R, Bidwell J, Wang Y, Pavalko F. Mechanical loading in osteocytes induces formation of a Src/Pyk2/MBD2 complex that suppresses anabolic gene expression. PLoS ONE. 2014;9:e97942 pubmed publisher
    ..The high bone mass phenotype of mice with global deletion of either Pyk2 or Src suggests a role for these tyrosine kinases in repression of bone formation...
  19. Timms J, Swanson K, Marie Cardine A, Raab M, Rudd C, Schraven B, et al. SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi-protein complexes in macrophages. Curr Biol. 1999;9:927-30 pubmed
    ..Fyn-binding protein/SLP-76-associated protein of 130 kDa), respectively, and the tyrosine kinase activity as PYK2. Two distinct SHPS-1 complexes were formed, one containing SKAP55hom/R and FYB/SLAP-130, and the other containing ..
  20. Alvau A, Battistone M, Gervasi M, Navarrete F, Xu X, Sanchez Cardenas C, et al. The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm. Development. 2016;143:2325-33 pubmed publisher
    ..Recent experiments using stallion and human sperm have suggested a role for PYK2 based on the use of small molecule inhibitors directed against this kinase...
  21. Lim S, Chen X, Tomar A, Miller N, Yoo J, Schlaepfer D. Knock-in mutation reveals an essential role for focal adhesion kinase activity in blood vessel morphogenesis and cell motility-polarity but not cell proliferation. J Biol Chem. 2010;285:21526-36 pubmed publisher
    ..This work supports the use of FAK inhibitors to disrupt neovascularization. ..
  22. Llewellyn R, Thomas K, Gutknecht M, Bouton A. The nonreceptor protein tyrosine kinase Pyk2 promotes the turnover of monocytes at steady state. J Leukoc Biol. 2017;102:1069-1080 pubmed publisher
    ..Proline-rich tyrosine kinase 2 (Pyk2) is a nonreceptor tyrosine kinase that regulates numerous immune cell functions, but its role in monocytes is ..
  23. Ruusala A, Aspenstrom P. The atypical Rho GTPase Wrch1 collaborates with the nonreceptor tyrosine kinases Pyk2 and Src in regulating cytoskeletal dynamics. Mol Cell Biol. 2008;28:1802-14 pubmed
    ..Here, we show that Wrch1 binds to the nonreceptor tyrosine kinase Pyk2. The interaction required Wrch1 to be in a GTP conformation and also required an intact N-terminal proline-rich ..
  24. Luo J, McGinnis L, Carlton C, Beggs H, Kinsey W. PTK2b function during fertilization of the mouse oocyte. Biochem Biophys Res Commun. 2014;450:1212-7 pubmed publisher
    ..of the fertilization-induced calcium transient is the calcium-calmodulin dependent protein tyrosine kinase PTK2b or PYK2 kinase...
  25. Canobbio I, Cipolla L, Guidetti G, Manganaro D, Visconte C, Kim S, et al. The focal adhesion kinase Pyk2 links Ca2+ signalling to Src family kinase activation and protein tyrosine phosphorylation in thrombin-stimulated platelets. Biochem J. 2015;469:199-210 pubmed publisher
    ..A23187 also triggered the phosphorylation and activation of the Ca(2+)-dependent focal adhesion kinase Pyk2 and Pyk2 activation by thrombin was Ca(2+)-dependent...
  26. Balasubramanian S, Quinones L, Kasiganesan H, Zhang Y, Pleasant D, Sundararaj K, et al. ?3 integrin in cardiac fibroblast is critical for extracellular matrix accumulation during pressure overload hypertrophy in mouse. PLoS ONE. 2012;7:e45076 pubmed publisher
    ..In addition, the activation of PDGF receptor associated tyrosine kinase and non-receptor tyrosine kinase Pyk2, upon PDGF stimulation were impaired in ?3-/- cells...
  27. Koshman Y, Chu M, Kim T, Kalmanson O, Farjah M, Kumar M, et al. Cardiomyocyte-specific expression of CRNK, the C-terminal domain of PYK2, maintains ventricular function and slows ventricular remodeling in a mouse model of dilated cardiomyopathy. J Mol Cell Cardiol. 2014;72:281-91 pubmed publisher
    Up-regulation and activation of PYK2, a member of the FAK family of protein tyrosine kinases, is involved in the pathogenesis of left ventricular (LV) remodeling and heart failure (HF)...
  28. Eleniste P, Du L, Shivanna M, Bruzzaniti A. Dynamin and PTP-PEST cooperatively regulate Pyk2 dephosphorylation in osteoclasts. Int J Biochem Cell Biol. 2012;44:790-800 pubmed publisher
    ..The tyrosine kinase Pyk2 is highly expressed in osteoclasts, and mice lacking Pyk2 exhibit an increase in bone mass, in part due to ..
  29. Suzuki C, Nakamura A, Miura N, Fukai K, Ohno N, Yahata T, et al. Non-receptor type, proline-rich protein tyrosine kinase 2 (Pyk2) is a possible therapeutic target for Kawasaki disease. Clin Immunol. 2017;179:17-24 pubmed publisher
    ..on experimental studies using a mouse model for KD, we report here that proline-rich protein tyrosine kinase 2 (Pyk2) plays a critical role in the onset of KD-like murine vasculitis...
  30. Fan H, Guan J. Compensatory function of Pyk2 protein in the promotion of focal adhesion kinase (FAK)-null mammary cancer stem cell tumorigenicity and metastatic activity. J Biol Chem. 2011;286:18573-82 pubmed publisher
    ..deletion in mammary tumor cells (MFCKO-MT mice), here we present evidence suggesting a compensatory function of Pyk2, a FAK-related kinase, in the regulation of MaCSCs and metastasis in these mice...
  31. Torigoe G, Nagao M, Tanabe N, Manaka S, Kariya T, Kawato T, et al. PYK2 mediates BzATP-induced extracellular matrix proteins synthesis. Biochem Biophys Res Commun. 2017;494:663-667 pubmed publisher
    ..Proline-rich tyrosine kinase 2 (PYK2) mediate osteoblasts differentiation is induced by mechanical stimuli...
  32. Le H, Maksumova L, Wang J, Pallen C. Reduced NMDA receptor tyrosine phosphorylation in PTPalpha-deficient mouse synaptosomes is accompanied by inhibition of four src family kinases and Pyk2: an upstream role for PTPalpha in NMDA receptor regulation. J Neurochem. 2006;98:1798-809 pubmed
    ..In addition, autophosphorylation of proline-rich tyrosine kinase 2 (Pyk2), a tyrosine kinase linked to NMDAR signaling, was also reduced in PTPalpha-deficient synaptosomes...
  33. Yoshida K, Yamashita Y, Miyazato A, Ohya K, Kitanaka A, Ikeda U, et al. Mediation by the protein-tyrosine kinase Tec of signaling between the B cell antigen receptor and Dok-1. J Biol Chem. 2000;275:24945-52 pubmed
    ..Furthermore, overexpression of Dok-1 inhibited activation of the c-fos promoter induced by stimulation of the BCR. These results suggest that Tec is an important mediator of signaling from the BCR to Dok-1. ..
  34. Manganaro D, Consonni A, Guidetti G, Canobbio I, Visconte C, Kim S, et al. Activation of phosphatidylinositol 3-kinase β by the platelet collagen receptors integrin α2β1 and GPVI: The role of Pyk2 and c-Cbl. Biochim Biophys Acta. 2015;1853:1879-88 pubmed publisher
    ..We have shown that the tyrosine kinase Pyk2 mediates PI3Kβ activation downstream of integrin αIIbβ3, and promotes the phosphorylation of the PI3K-..
  35. Fukai K, Nakamura A, Hoshino A, Nakanishi N, Okawa Y, Ariyoshi M, et al. Pyk2 aggravates hypoxia-induced pulmonary hypertension by activating HIF-1α. Am J Physiol Heart Circ Physiol. 2015;308:H951-9 pubmed publisher
    ..In the present study, we demonstrated that proline-rich tyrosine kinase 2 (Pyk2), a nonreceptor type protein tyrosine kinase, plays a crucial role in the pathogenesis of pulmonary hypertension (..
  36. Kruljac Letunic A, Moelleken J, Kallin A, Wieland F, Blaukat A. The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1. J Biol Chem. 2003;278:29560-70 pubmed
    Proline-rich tyrosine kinase 2 (Pyk2), a non-receptor tyrosine kinase structurally related to focal adhesion kinase, has been implicated in the regulation of mitogen-activated protein kinase cascades and ion channels, the induction of ..
  37. Zhao H, Liu X, Zou H, Dai N, Yao L, Zhang X, et al. Osteoprotegerin disrupts peripheral adhesive structures of osteoclasts by modulating Pyk2 and Src activities. Cell Adh Migr. 2016;10:299-309 pubmed publisher
    ..Combined imaging and Western blot studies showed that osteoprotegerin induced dephosphorylation of Tyr 402 in Pyk2 and decreased its labeling in peripheral adhesion regions...
  38. Ray B, Thomas K, Huang C, Gutknecht M, Botchwey E, Bouton A. Regulation of osteoclast structure and function by FAK family kinases. J Leukoc Biol. 2012;92:1021-8 pubmed publisher
    ..To this end, we investigated unique and overlapping functions of members of the FAK family (FAK and Pyk2) in osteoclast functions...
  39. Yamamoto S, Shimizu S, Kiyonaka S, Takahashi N, Wajima T, Hara Y, et al. TRPM2-mediated Ca2+influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration. Nat Med. 2008;14:738-47 pubmed publisher
    ..hydrogen peroxide (H(2)O(2)) evokes Ca(2+) influx through TRPM2 to activate Ca(2+)-dependent tyrosine kinase Pyk2 and amplify Erk signaling via Ras GTPase...
  40. Roy S, Ruest P, Hanks S. FAK regulates tyrosine phosphorylation of CAS, paxillin, and PYK2 in cells expressing v-Src, but is not a critical determinant of v-Src transformation. J Cell Biochem. 2002;84:377-88 pubmed
    ..FAK was also shown to negatively regulate v-Src mediated phosphorylation of the FAK-related kinase PYK2. Despite these effects, FAK does not play an essential role in targeting v-Src to major cellular substrates ..
  41. Meng X, Dai Y, Jing L, Bai J, Liu S, Zheng K, et al. Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice. J Reprod Dev. 2016;62:351-8 pubmed publisher
    Proline-rich tyrosine kinase 2 (Pyk2), a non-receptor tyrosine kinase, is a member of the focal adhesion kinase family and is highly expressed in oocytes...
  42. Fisslthaler B, Loot A, Mohamed A, Busse R, Fleming I. Inhibition of endothelial nitric oxide synthase activity by proline-rich tyrosine kinase 2 in response to fluid shear stress and insulin. Circ Res. 2008;102:1520-8 pubmed publisher
    ..We found that fluid shear stress induces the association of eNOS with the proline-rich tyrosine kinase 2 (PYK2) in endothelial cells and that the eNOS immunoprecipitated from eNOS- and PYK2-overexpressing HEK293 cells was ..
  43. Wang L, Tassiulas I, Park Min K, Reid A, Gil Henn H, Schlessinger J, et al. 'Tuning' of type I interferon-induced Jak-STAT1 signaling by calcium-dependent kinases in macrophages. Nat Immunol. 2008;9:186-93 pubmed
    ..Here we investigated the function of the calcium-dependent kinases CaMK and Pyk2 'downstream' of ITAM-associated receptors in the regulation of cytokine-induced activation of Jak kinases and STAT ..
  44. Fisher K, Codina J, Petrovic S, Dubose T. Pyk2 regulates H+-ATPase-mediated proton secretion in the outer medullary collecting duct via an ERK1/2 signaling pathway. Am J Physiol Renal Physiol. 2012;303:F1353-62 pubmed publisher
    ..Little is known about the mechanism by which these cells sense changes in extracellular pH (pH(o)). Pyk2 is a nonreceptor tyrosine kinase activated by autophosphorylation at Tyr402 by cell-specific stimuli, including ..
  45. Beinke S, Phee H, Clingan J, Schlessinger J, Matloubian M, Weiss A. Proline-rich tyrosine kinase-2 is critical for CD8 T-cell short-lived effector fate. Proc Natl Acad Sci U S A. 2010;107:16234-9 pubmed publisher
    ..Here we demonstrate that proline-rich tyrosine kinase-2 (PYK2) deficiency impairs CD8 T-cell activation by synergistic LFA-1 and T-cell receptor stimulation...
  46. Benzing T, Gerke P, Höpker K, Hildebrandt F, Kim E, Walz G. Nephrocystin interacts with Pyk2, p130(Cas), and tensin and triggers phosphorylation of Pyk2. Proc Natl Acad Sci U S A. 2001;98:9784-9 pubmed
    ..of nephrocystin revealed that nephrocystin forms protein complexes with p130(Cas), proline-rich tyrosine kinase 2 (Pyk2), and tensin, indicating that these proteins participate in a common signaling pathway...
  47. Chieffi P, Barchi M, Di Agostino S, Rossi P, Tramontano D, Geremia R. Prolin-rich tyrosine kinase 2 (PYK2) expression and localization in mouse testis. Mol Reprod Dev. 2003;65:330-5 pubmed
    Prolin-rich kinase 2 (PYK2) is a nonreceptor tyrosine kinase related to the focal adhesion kinase (FAK) p125(FAK)...
  48. Peng X, Kraus M, Wei H, Shen T, Pariaut R, Alcaraz A, et al. Inactivation of focal adhesion kinase in cardiomyocytes promotes eccentric cardiac hypertrophy and fibrosis in mice. J Clin Invest. 2006;116:217-27 pubmed
    ..Together, these results provide strong evidence for a role of FAK in the regulation of heart hypertrophy in vivo. ..
  49. Paone C, Rodrigues N, Ittner E, Santos C, Buntru A, Hauck C. The Tyrosine Kinase Pyk2 Contributes to Complement-Mediated Phagocytosis in Murine Macrophages. J Innate Immun. 2016;8:437-51 pubmed publisher
    Proline-rich tyrosine kinase 2 (Pyk2) is a member of the focal adhesion kinase (FAK) family and is mainly expressed in neuronal and hematopoietic cells...
  50. Soni D, Regmi S, Wang D, Debroy A, Zhao Y, Vogel S, et al. Pyk2 phosphorylation of VE-PTP downstream of STIM1-induced Ca2+ entry regulates disassembly of adherens junctions. Am J Physiol Lung Cell Mol Physiol. 2017;312:L1003-L1017 pubmed publisher
    ..We observed that SOCE induced by STIM1 activated Pyk2 in human lung microvascular endothelial cells (ECs) and induced tyrosine phosphorylation of VE-PTP at Y1981...
  51. Cao Y, Baig M, Hamm L, Wu K, Simon E. Growth factors stimulate kidney proximal tubule cell migration independent of augmented tyrosine phosphorylation of focal adhesion kinase. Biochem Biophys Res Commun. 2005;328:560-6 pubmed
    ..Thus, in proximal tubule cells, stimulation of cell migration by growth factors was independent of augmented FAK tyrosine phosphorylation. ..
  52. Moissoglu K, Gelman I. v-Src rescues actin-based cytoskeletal architecture and cell motility and induces enhanced anchorage independence during oncogenic transformation of focal adhesion kinase-null fibroblasts. J Biol Chem. 2003;278:47946-59 pubmed
    ..Our data also indicate that the enhanced Pyk2 level found in FAK-/- MEF plays no role in v-Src-induced anchorage independence...
  53. Cheung S, Ostergaard H. Pyk2 Controls Integrin-Dependent CTL Migration through Regulation of De-Adhesion. J Immunol. 2016;197:1945-56 pubmed publisher
    Protein tyrosine kinase 2 (Pyk2) is required for T cell adhesion to ICAM-1; however, the mechanism by which it regulates adhesion remains unexplored...
  54. Lee E, Kim J, Gong J, Park S, Kang Y. Inhibition of osteoclast activation by phloretin through disturbing αvβ3 integrin-c-Src pathway. Biomed Res Int. 2015;2015:680145 pubmed publisher
    ..The RANKL induction of actin rings and vacuolar-type H(+)-ATPase entailed Pyk2 phosphorylation and c-Src and c-Cbl induction, all of which were blunted by phloretin...
  55. Masuhara M, Nagao K, Nishikawa M, Sasaki M, Yoshimura A, Osawa M. Molecular cloning of murine STAP-1, the stem-cell-specific adaptor protein containing PH and SH2 domains. Biochem Biophys Res Commun. 2000;268:697-703 pubmed
    ..A two-hybrid assay indicated that STAP-1 bound not only to c-kit but also to c-fms but not to JAK2 or Pyk2. In 293 cells, STAP-1 was tyrosine-phosphorylated by activated c-kit...
  56. Carrim N, Walsh T, Consonni A, Torti M, Berndt M, Metharom P. Role of focal adhesion tyrosine kinases in GPVI-dependent platelet activation and reactive oxygen species formation. PLoS ONE. 2014;9:e113679 pubmed publisher
    We have previously shown the presence of a TRAF4/p47phox/Hic5/Pyk2 complex associated with the platelet collagen receptor, GPVI, consistent with a potential role of this complex in GPVI-dependent ROS formation...
  57. Xi C, Xiong F, Zhou Z, Mei L, Xiong W. PYK2 interacts with MyD88 and regulates MyD88-mediated NF-kappaB activation in macrophages. J Leukoc Biol. 2010;87:415-23 pubmed publisher
    b>PYK2, a major cell adhesion-activated tyrosine kinase, is highly expressed in macrophages and implicated in macrophage activation and inflammatory response...
  58. Hakim Z, DiMichele L, Doherty J, Homeister J, Beggs H, Reichardt L, et al. Conditional deletion of focal adhesion kinase leads to defects in ventricular septation and outflow tract alignment. Mol Cell Biol. 2007;27:5352-64 pubmed
    ..Future studies will be necessary to determine the precise contributions of the additional nkx2-5-derived lineages to the phenotypes observed. ..
  59. Andreev J, Galisteo M, Kranenburg O, Logan S, Chiu E, Okigaki M, et al. Src and Pyk2 mediate G-protein-coupled receptor activation of epidermal growth factor receptor (EGFR) but are not required for coupling to the mitogen-activated protein (MAP) kinase signaling cascade. J Biol Chem. 2001;276:20130-5 pubmed
    The epidermal growth factor receptor (EGFR) and the non-receptor protein tyrosine kinases Src and Pyk2 have been implicated in linking a variety of G-protein-coupled receptors (GPCR) to the mitogen-activated protein (MAP) kinase signaling ..
  60. Shen C, Raghavan S, Xu Z, Baranski J, Yu X, Wozniak M, et al. Decreased cell adhesion promotes angiogenesis in a Pyk2-dependent manner. Exp Cell Res. 2011;317:1860-71 pubmed publisher
    ..in adhesion signaling previously implicated in angiogenesis, and its homologue, proline-rich tyrosine kinase 2 (Pyk2)...
  61. Wang H, Luo J, Carlton C, McGinnis L, Kinsey W. Sperm-oocyte contact induces outside-in signaling via PYK2 activation. Dev Biol. 2017;428:52-62 pubmed publisher
    ..While it is known that proline - rich tyrosine kinase 2 (PYK2 or PTK2b) kinase activity plays an important role in fertilization, its specific function has not been addressed...
  62. Eleniste P, Patel V, Posritong S, Zero O, Largura H, Cheng Y, et al. Pyk2 and Megakaryocytes Regulate Osteoblast Differentiation and Migration Via Distinct and Overlapping Mechanisms. J Cell Biochem. 2016;117:1396-406 pubmed publisher
    ..Mice lacking the proline-rich tyrosine kinase Pyk2 (Pyk2-KO) have increased bone mass, in part due to increased osteoblast proliferation...
  63. Chung I, OuYang C, Yuan S, Li H, Chen J, Shieh H, et al. Pyk2 activates the NLRP3 inflammasome by directly phosphorylating ASC and contributes to inflammasome-dependent peritonitis. Sci Rep. 2016;6:36214 pubmed publisher
    ..Here, we report that the cytosolic tyrosine kinases, FAK and Pyk2, are differentially involved in NLRP3 and AIM2 inflammasome activation...
  64. Marqués M, Kumar A, Poveda A, Zuluaga S, Hernandez C, Jackson S, et al. Specific function of phosphoinositide 3-kinase beta in the control of DNA replication. Proc Natl Acad Sci U S A. 2009;106:7525-30 pubmed publisher
    ..Also, p110beta associated with PCNA and controlled PCNA loading onto chromatin in a kinase-independent manner. These results show a selective function of PI3Kbeta in the control of DNA replication. ..
  65. Lakkakorpi P, Nakamura I, Nagy R, Parsons J, Rodan G, Duong L. Stable association of PYK2 and p130(Cas) in osteoclasts and their co-localization in the sealing zone. J Biol Chem. 1999;274:4900-7 pubmed
    ..The present study examines the interaction between PYK2 and p130(Cas) (Crk-associated substrate), suggested to be part of the signaling pathway initiated by osteoclast ..
  66. Rhee I, Davidson D, Souza C, Vacher J, Veillette A. Macrophage fusion is controlled by the cytoplasmic protein tyrosine phosphatase PTP-PEST/PTPN12. Mol Cell Biol. 2013;33:2458-69 pubmed publisher
    ..PTP-PEST deficiency resulted in specific hyperphosphorylation of the protein tyrosine kinase Pyk2 and the adaptor paxillin...
  67. Kacena M, Eleniste P, Cheng Y, Huang S, Shivanna M, Meijome T, et al. Megakaryocytes regulate expression of Pyk2 isoforms and caspase-mediated cleavage of actin in osteoblasts. J Biol Chem. 2012;287:17257-68 pubmed publisher
    ..The tyrosine kinase Pyk2 has been shown to regulate cell proliferation and survival in a variety of cells...
  68. Xie J, Allen K, Marguet A, Berghorn K, Bliss S, Navratil A, et al. Analysis of the calcium-dependent regulation of proline-rich tyrosine kinase 2 by gonadotropin-releasing hormone. Mol Endocrinol. 2008;22:2322-35 pubmed publisher
    ..Phosphorylated Pyk2 was present in alphaT3-1 cells after GnRH agonist administration for a time course that lasted up to 4 h...
  69. Sanger J, Wang J, Gleason L, Chowrashi P, Dube D, Mittal B, et al. Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules. Cytoskeleton (Hoboken). 2010;67:808-23 pubmed publisher
    ..Our results indicate that ArgBP2 may play an important role in the assembly and maintenance of myofibrils in cardiomyocytes. ..
  70. Chen J, Lu Y, Meng S, Han M, Lin C, Wang X. alpha- and gamma-Protocadherins negatively regulate PYK2. J Biol Chem. 2009;284:2880-90 pubmed publisher
    ..Here, we show that PCDH-gamma binds two tyrosine kinases, PYK2 and focal adhesion kinase (FAK), and interaction with PCDH-gamma inhibits kinase activity...
  71. Duan Y, Learoyd J, Meliton A, Clay B, Leff A, Zhu X. Inhibition of Pyk2 blocks airway inflammation and hyperresponsiveness in a mouse model of asthma. Am J Respir Cell Mol Biol. 2010;42:491-7 pubmed publisher
    The objective of this investigation was to determine the role of Pyk2, an intracellular nonreceptor protein tyrosine kinase for postadhesive inflammatory cell migration, on airway inflammation and hyperresponsiveness in immune-sensitized ..
  72. Tsutsumi K, Yamaura T, Nakajima M, Honda T, Kasaoka T. Silencing of focal adhesion kinase by tumor direct injection of small interfering RNA decreases in vivo tumor growth. Cancer Biol Ther. 2009;8:1292-9 pubmed
    ..In addition, we showed that ERK is the critical MAP kinase in the signaling pathway down stream of FAK in in vivo proliferation of 4T1 tumor cells. ..
  73. Tomar A, Lim S, Lim Y, Schlaepfer D. A FAK-p120RasGAP-p190RhoGAP complex regulates polarity in migrating cells. J Cell Sci. 2009;122:1852-62 pubmed publisher
    ..Because reconstitution of FAK-null fibroblasts with FAK or a Pyk2-FAK chimera restore the normal decrease in RhoA GTP binding upon cell spreading on fibronectin, our studies support ..
  74. Kinoshita Y, Hunter R, Gray J, Mesias R, McEwen B, Benson D, et al. Role for NUP62 depletion and PYK2 redistribution in dendritic retraction resulting from chronic stress. Proc Natl Acad Sci U S A. 2014;111:16130-5 pubmed publisher
    ..In cultured cells, phosphorylation of a FAK/proline-rich tyrosine kinase 2 (PYK2) consensus site in the alpha-helical domain of NUP62 (human Y422) is shown to be associated with shedding of NUP62 ..
  75. Yeon J, Ryu B, Choi S, Heo J, Kim K, Son Y, et al. Natural polyamines inhibit the migration of preosteoclasts by attenuating Ca2+-PYK2-Src-NFATc1 signaling pathways. Amino Acids. 2014;46:2605-14 pubmed publisher
    ..Furthermore, the RANKL-mediated activation of the Src-PYK2 signaling axis and of transcription factors such as NF-κB and NFATc1 was prevented by each polyamine...
  76. Cheng Y, Hooker R, Nguyen K, Gerard O Riley R, Waning D, Chitteti B, et al. Pyk2 regulates megakaryocyte-induced increases in osteoblast number and bone formation. J Bone Miner Res. 2013;28:1434-45 pubmed publisher
    ..We, therefore, examined the role of Pyk2, a tyrosine kinase known to be regulated downstream of integrins, in the MK-mediated enhancement of OBs...
  77. Sonomura K, Okigaki M, Kimura T, Matsuoka E, Shiotsu Y, Adachi T, et al. The kinase Pyk2 is involved in renal fibrosis by means of mechanical stretch-induced growth factor expression in renal tubules. Kidney Int. 2012;81:449-57 pubmed publisher
    ..stretch induced reactive oxygen species that in turn activated the cytoplasmic proline-rich tyrosine kinase-2 (Pyk2). This kinase is abundantly expressed in tubular epithelial cells where it is activated by several stimuli...
  78. Ding Q, Gladson C, Wu H, Hayasaka H, Olman M. Focal adhesion kinase (FAK)-related non-kinase inhibits myofibroblast differentiation through differential MAPK activation in a FAK-dependent manner. J Biol Chem. 2008;283:26839-49 pubmed publisher
    ..These data further suggest that modulation FRNK expression may be a novel avenue for therapeutic intervention in tissue fibrosis. ..