Ppp1r12a

Summary

Gene Symbol: Ppp1r12a
Description: protein phosphatase 1, regulatory (inhibitor) subunit 12A
Alias: 1200015F06Rik, 5730577I22Rik, AA792106, AV099298, D10Ertd625e, Mypt1, myosin phosphatase target subunit 1, myosin phosphatase, target subunit 1, myosin phosphatase-targeting subunit 1, protein phosphatase 1 regulatory subunit 12A
Species: mouse

Top Publications

  1. ncbi Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene
    H Machida
    First Department of Internal Medicine, University School of Medicine, Tsu, Mie, Japan
    Biochim Biophys Acta 1517:424-9. 2001
  2. doi ROS-mediated downregulation of MYPT1 in smooth muscle cells: a potential mechanism for the aberrant contractility in atherosclerosis
    Jung Chien Cheng
    Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Lab Invest 93:422-33. 2013
  3. pmc Tra2β protein is required for tissue-specific splicing of a smooth muscle myosin phosphatase targeting subunit alternative exon
    Kang Fu
    Department of Medicine Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 287:16575-85. 2012
  4. doi Impaired vascular remodeling in the yolk sac of embryos deficient in ROCK-I and ROCK-II
    Hiroshi Kamijo
    Department of Pharmacology, Kyoto University, Japan
    Genes Cells 16:1012-21. 2011
  5. pmc Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis
    Deqiang Li
    Riley Heart Research Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Development 138:303-15. 2011
  6. pmc Smoothelin-like 1 protein regulates myosin phosphatase-targeting subunit 1 expression during sexual development and pregnancy
    Beata Lontay
    Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 285:29357-66. 2010
  7. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
  8. pmc Regulation of basal LC20 phosphorylation by MYPT1 and CPI-17 in murine gastric antrum, gastric fundus, and proximal colon smooth muscles
    B P Bhetwal
    Department of Physiology and Cell Biology, Center of Biomedical Research Excellence, University of Nevada School of Medicine, 1664 N Virginia St, Reno, NV 89557, USA
    Neurogastroenterol Motil 23:e425-36. 2011
  9. pmc Altered contractile phenotypes of intestinal smooth muscle in mice deficient in myosin phosphatase target subunit 1
    Wei Qi He
    Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China
    Gastroenterology 144:1456-65, 1465.e1-5. 2013
  10. ncbi Preserved expression of GLUT4 prevents enhanced agonist-induced vascular reactivity and MYPT1 phosphorylation in hypertensive mouse aorta
    Kevin B Atkins
    Department of Internal Medicine, University of Michigan Medical School, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0676, USA
    Am J Physiol Heart Circ Physiol 293:H402-8. 2007

Scientific Experts

  • Kevin B Atkins
  • Junji Yamauchi
  • David J Hartshorne
  • Jung Chien Cheng
  • Wei Qi He
  • Kang Fu
  • Deqiang Li
  • B P Bhetwal
  • Hiroshi Kamijo
  • Yaopan Mao
  • Kristine E Kamm
  • James T Stull
  • Steven A Fisher
  • Dou Dou
  • Beata Lontay
  • Jee In Kim
  • Rowena Suriben
  • Win D Cheung
  • Supriya Shukla
  • Yusuke Mizuno
  • Nicole Harb
  • Daisuke Umeda
  • Haruhiko Tokuda
  • Maribel Parra
  • Lubomir T Lubomirov
  • Masaaki Ito
  • Yue Wu
  • Ryuji Okamoto
  • Kenta Kobayashi
  • Pei Wang
  • Chao Jun Li
  • Yan Ning Qiao
  • Cai Ping Chen
  • Hui Pin Cheng
  • I Ching Tsai
  • Meei Jyh Jiang
  • Ya Jing Peng
  • Chen Chen
  • Xiao Yang
  • Min Sheng Zhu
  • Juan Min Zha
  • Cheng Hai Zhang
  • Salah Baker
  • H Machida
  • Brunhilde Wirth
  • Brian A Perrino
  • Ylva Mende
  • Bhupal P Bhetwal
  • Katherine Malanga Morgan
  • Weinian Shou
  • Ying Liu
  • Michael Rubart
  • Kenneth D Irvine
  • Shuh Narumiya
  • S A Fisher
  • Dean Thumkeo
  • Seiichi Koike
  • Hanying Chen
  • Mark A Hallett
  • Rebecca J Chan
  • Zhenyun Yang
  • M Albert Basson
  • Laura S Haneline
  • Joanna Mulvaney
  • C L An
  • Yoshihiko Shimizu
  • Toshimasa Ishizaki
  • Yutaka Matsumura
  • Sana Zakaria
  • Robert J Schwartz
  • B A Perrino
  • Philippa Francis-West
  • Loren J Field
  • Masayuki Masu
  • Wuqiang Zhu
  • Simon J Atkinson
  • Steve Allen
  • Tian Yu
  • Douglas H Weitzel
  • Lei Ying
  • Xiaoxing Yu
  • Huijuan Ma
  • Xiaoxu Zheng
  • James Devente
  • Timothy A J Haystead
  • David Loiselle
  • Robert Hickner
  • Yuansheng Gao
  • Szabolcs Lengyel
  • Donghai Zheng

Detail Information

Publications27

  1. ncbi Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene
    H Machida
    First Department of Internal Medicine, University School of Medicine, Tsu, Mie, Japan
    Biochim Biophys Acta 1517:424-9. 2001
    We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site...
  2. doi ROS-mediated downregulation of MYPT1 in smooth muscle cells: a potential mechanism for the aberrant contractility in atherosclerosis
    Jung Chien Cheng
    Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Lab Invest 93:422-33. 2013
    ..smooth muscle relaxation, is regulated by the phosphorylation of its regulatory subunit, MP targeting subunit 1 (MYPT1)...
  3. pmc Tra2β protein is required for tissue-specific splicing of a smooth muscle myosin phosphatase targeting subunit alternative exon
    Kang Fu
    Department of Medicine Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 287:16575-85. 2012
    Alternative splicing of the smooth muscle myosin phosphatase targeting subunit (Mypt1) exon 23 (E23) is tissue-specific and developmentally regulated and, thus, an attractive model for the study of smooth muscle phenotypic specification...
  4. doi Impaired vascular remodeling in the yolk sac of embryos deficient in ROCK-I and ROCK-II
    Hiroshi Kamijo
    Department of Pharmacology, Kyoto University, Japan
    Genes Cells 16:1012-21. 2011
    ..These results suggest that ROCK isoforms function redundantly during embryogenesis and play a critical role in vascular development...
  5. pmc Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis
    Deqiang Li
    Riley Heart Research Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Development 138:303-15. 2011
    ..Biochemical analyses indicate that Daam1 does not regulate cytoskeletal organization through RhoA, Rac1 or Cdc42. Our study highlights a crucial role for Daam1 in regulating the actin cytoskeleton and tissue morphogenesis...
  6. pmc Smoothelin-like 1 protein regulates myosin phosphatase-targeting subunit 1 expression during sexual development and pregnancy
    Beata Lontay
    Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 285:29357-66. 2010
    ..A primary target gene of SMTNL1 in these muscles is myosin phosphatase-targeting subunit 1 (MYPT1)...
  7. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
    ..Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase...
  8. pmc Regulation of basal LC20 phosphorylation by MYPT1 and CPI-17 in murine gastric antrum, gastric fundus, and proximal colon smooth muscles
    B P Bhetwal
    Department of Physiology and Cell Biology, Center of Biomedical Research Excellence, University of Nevada School of Medicine, 1664 N Virginia St, Reno, NV 89557, USA
    Neurogastroenterol Motil 23:e425-36. 2011
    ..Here, we investigate the role of ROK in regulating LC20 phosphorylation and spontaneous contractions of gastric fundus, gastric antrum, and proximal colon smooth muscles...
  9. pmc Altered contractile phenotypes of intestinal smooth muscle in mice deficient in myosin phosphatase target subunit 1
    Wei Qi He
    Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China
    Gastroenterology 144:1456-65, 1465.e1-5. 2013
    The regulatory subunit of myosin light chain phosphatase, MYPT1, has been proposed to control smooth muscle contractility by regulating phosphorylation of the Ca(2+)-dependent myosin regulatory light chain...
  10. ncbi Preserved expression of GLUT4 prevents enhanced agonist-induced vascular reactivity and MYPT1 phosphorylation in hypertensive mouse aorta
    Kevin B Atkins
    Department of Internal Medicine, University of Michigan Medical School, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0676, USA
    Am J Physiol Heart Circ Physiol 293:H402-8. 2007
    ..Phosphorylation of the myosin phosphatase targeting subunit MYPT1 was significantly enhanced in aortas of WT DOCA-salt mice, and this increase was prevented in GLUT4 TG mice...
  11. ncbi Capturing novel mouse genes encoding chromosomal and other nuclear proteins
    P Tate
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, Scotland, UK
    J Cell Sci 111:2575-85. 1998
    ..Motifs in two of the isolated genes suggest new links between cellular regulatory mechanisms (ubiquitination and phosphorylation) and mRNA splicing and chromosome structure/function...
  12. ncbi Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase
    Kenta Kobayashi
    Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960 1295, Japan
    J Neurosci 24:3480-8. 2004
    ..These findings indicate that the Rho signaling pathway, primarily through Rho-kinase, plays a crucial role in survival of spinal MNs during embryogenesis, particularly at the early developmental stages...
  13. ncbi Localization of myosin phosphatase target subunit and its mutants
    Yue Wu
    Muscle Biology Group, University of Arizona, Tucson, AZ 85721, USA
    J Muscle Res Cell Motil 26:123-34. 2005
    Transient transfection of NIH3T3 cells with various constructs of myosin phosphatase target subunit (MYPT1) and GFP showed distinct cellular localizations. Constructs containing the N-terminal nuclear localization signals (NLS), i.e...
  14. doi The atypical Guanine-nucleotide exchange factor, dock7, negatively regulates schwann cell differentiation and myelination
    Junji Yamauchi
    Department of Pharmacology and Laboratory Animal Resource Facility, National Research Institute for Child Health and Development, Setagaya, Tokyo 157 8535, Japan
    J Neurosci 31:12579-92. 2011
    ..Collectively, the signaling through Dock7 negatively regulates Schwann cell differentiation and the onset of myelination, demonstrating the unexpected role of Dock7 in the interplay between Schwann cell migration and myelination...
  15. pmc Characterization of a Dchs1 mutant mouse reveals requirements for Dchs1-Fat4 signaling during mammalian development
    Yaopan Mao
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
    Development 138:947-57. 2011
    ..Our analysis implies that Dchs1 and Fat4 function as a ligand-receptor pair during murine development, and identifies novel requirements for Dchs1-Fat4 signaling in multiple organs...
  16. ncbi The targeted disruption of the MYPT1 gene results in embryonic lethality
    Ryuji Okamoto
    The First Department of Internal Medicine, Mie University School of Medicine, 2 174 Edobashi, Tsu, Mie 514 8507, Japan
    Transgenic Res 14:337-40. 2005
    ..b>MYPT1, a target subunit of smooth and nonmuscle MP, is responsible for activation and regulation of MP...
  17. ncbi Urocortin-induced decrease in Ca2+ sensitivity of contraction in mouse tail arteries is attributable to cAMP-dependent dephosphorylation of MYPT1 and activation of myosin light chain phosphatase
    Lubomir T Lubomirov
    Institute of Vegetative Physiology, University of Cologne, Germany
    Circ Res 98:1159-67. 2006
    ..a approximately 2-fold increase in the phosphorylation of the regulatory subunit of myosin phosphatase (SMPP-1M), MYPT1, at Thr696, which was reversed in arteries relaxed with urocortin. Submaximally (pCa 6...
  18. doi Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance
    Dou Dou
    Department of Physiology and Pathophysiology, Peking University Health Science Center, 38 Xue Yuan Road, Beijing 100191, People s Republic of China
    Cardiovasc Res 86:151-9. 2010
    ..The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance.
  19. pmc Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak
    Rowena Suriben
    Department of Psychiatry, University of California, San Francisco, San Francisco, California, USA
    Nat Genet 41:977-85. 2009
    ..We conclude that Dact1 contributes to morphogenesis at the primitive streak by regulating Vangl2 upstream of cell adhesion and the PCP pathway...
  20. pmc Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation
    Jee In Kim
    Department of Molecular Physiology and Biophysics, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA
    Histochem Cell Biol 132:191-8. 2009
    ..Thus, CPI-17 expression is reversibly controlled in response to the phenotype transition of SM cells that restricts the signal to differentiated SM cells and particular cell types...
  21. pmc O-linked beta-N-acetylglucosaminyltransferase substrate specificity is regulated by myosin phosphatase targeting and other interacting proteins
    Win D Cheung
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 283:33935-41. 2008
    ..We demonstrate here that MYPT1 and CARM1 also interact with and target OGT. MYPT1 and CARM1 are substrates of OGT in vitro and in vivo...
  22. pmc Myosin phosphatase dephosphorylates HDAC7, controls its nucleocytoplasmic shuttling, and inhibits apoptosis in thymocytes
    Maribel Parra
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, California 94158, USA
    Genes Dev 21:638-43. 2007
    ..Here, we identify protein phosphatase 1beta (PP1beta) and myosin phosphatase targeting subunit 1 (MYPT1), two components of the myosin phosphatase complex, as HDAC7-associated proteins in ..
  23. pmc Tra2beta as a novel mediator of vascular smooth muscle diversification
    Supriya Shukla
    Departments of Medicine Cardiology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106, USA
    Circ Res 103:485-92. 2008
    ..We are using the alternative splicing of the MYPT1 E23 exon as a model for the study of smooth muscle diversification into fast and slow contractile phenotypes...
  24. pmc Myosin light chain kinase activation and calcium sensitization in smooth muscle in vivo
    Yusuke Mizuno
    Dept Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    Am J Physiol Cell Physiol 295:C358-64. 2008
    ..may occur through RhoA/Rho-kinase and/or PKC with phosphorylation of myosin phosphatase targeting subunit-1 (MYPT1) and PKC-potentiated phosphatase inhibitor (CPI-17), respectively...
  25. ncbi Green tea polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor
    Daisuke Umeda
    Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812 8581, Japan
    J Biol Chem 283:3050-8. 2008
    ..activity of EGCG; (ii) through both eEF1A and 67LR, EGCG induces the dephosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) at Thr-696 and activates myosin phosphatase; and (iii) silencing of 67LR, eEF1A, or MYPT1 ..
  26. ncbi Rho-kinase regulates endothelin-1-stimulated IL-6 synthesis via p38 MAP kinase in osteoblasts
    Haruhiko Tokuda
    Department of Clinical Laboratory, National Hospital for Geriatric Medicine, National Center for Geriatrics and Gerontology, Obu, Aichi 474 8511, and Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501 1194, Japan
    Biochem Biophys Res Commun 362:799-804. 2007
    ..These results strongly suggest that Rho-kinase regulates ET-1-stimulated IL-6 synthesis through p38 MAP kinase activation in osteoblasts...
  27. pmc The Rho-Rock-Myosin signaling axis determines cell-cell integrity of self-renewing pluripotent stem cells
    Nicole Harb
    Department of Biochemistry, University of California Riverside, Riverside, California, United States of America
    PLoS ONE 3:e3001. 2008
    ..Although intercellular communications are essential to establish the basis of cell-specific identity, molecular mechanisms underlying intrinsic cell-cell interactions in ES cells at the signaling level remain underexplored...