Gene Symbol: Pou3f1
Description: POU domain, class 3, transcription factor 1
Alias: Oct-6, Oct6, Otf-6, Otf6, Scip, Test1, Tst-1, Tst1, POU domain, class 3, transcription factor 1, POU domain transcription factor SCIP, octamer binding factor oct6, octamer-binding protein 6, octamer-binding transcription factor 6
Species: mouse
Products:     Pou3f1

Top Publications

  1. Benninger Y, Thurnherr T, Pereira J, Krause S, Wu X, Chrostek Grashoff A, et al. Essential and distinct roles for cdc42 and rac1 in the regulation of Schwann cell biology during peripheral nervous system development. J Cell Biol. 2007;177:1051-61 pubmed
    ..Our results indicate that although cdc42 is required for normal Schwann cell proliferation, rac1 regulates Schwann cell process extension and stabilization, allowing efficient radial sorting of axon bundles...
  2. Ryu E, Wang J, Le N, Baloh R, Gustin J, Schmidt R, et al. Misexpression of Pou3f1 results in peripheral nerve hypomyelination and axonal loss. J Neurosci. 2007;27:11552-9 pubmed
    b>Pou3f1/SCIP/Oct-6 is a POU-domain transcription factor that is an important regulator of peripheral nerve myelination by Schwann cells...
  3. Bermingham J, Scherer S, O Connell S, Arroyo E, Kalla K, Powell F, et al. Tst-1/Oct-6/SCIP regulates a unique step in peripheral myelination and is required for normal respiration. Genes Dev. 1996;10:1751-62 pubmed
    ..The POU domain transcription factor Tst-1/Oct-6/SCIP is expressed transiently during myelination, and we report here that it has a critical role in this developmental ..
  4. Schonemann M, Ryan A, McEvilly R, O Connell S, Arias C, Kalla K, et al. Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2. Genes Dev. 1995;9:3122-35 pubmed
  5. Hara Y, Rovescalli A, Kim Y, Nirenberg M. Structure and evolution of four POU domain genes expressed in mouse brain. Proc Natl Acad Sci U S A. 1992;89:3280-4 pubmed
    Four mouse POU domain genomic DNA clones--Brain-1, Brain-2, Brain-4, and Scip--and Brain-2 cDNA, which are expressed in adult brain, were cloned and the coding and noncoding regions of the genes were sequenced...
  6. Jaegle M, Mandemakers W, Broos L, Zwart R, Karis A, Visser P, et al. The POU factor Oct-6 and Schwann cell differentiation. Science. 1996;273:507-10 pubmed
    The POU transcription factor Oct-6, also known as SCIP or Tst-1, has been implicated as a major transcriptional regulator in Schwann cell differentiation...
  7. Jaegle M, Ghazvini M, Mandemakers W, Piirsoo M, Driegen S, Levavasseur F, et al. The POU proteins Brn-2 and Oct-6 share important functions in Schwann cell development. Genes Dev. 2003;17:1380-91 pubmed
    The genetic hierarchy that controls myelination of peripheral nerves by Schwann cells includes the POU domain Oct-6/Scip/Tst-1and the zinc-finger Krox-20/Egr2 transcription factors...
  8. Zwart R, Broos L, Grosveld G, Meijer D. The restricted expression pattern of the POU factor Oct-6 during early development of the mouse nervous system. Mech Dev. 1996;54:185-94 pubmed
  9. Finzsch M, Schreiner S, Kichko T, Reeh P, Tamm E, Bösl M, et al. Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage. J Cell Biol. 2010;189:701-12 pubmed publisher
    ..Schwann cells failed to develop beyond the immature stage and were unable to maintain identity. Thus, our study identifies a novel cause for peripheral neuropathies in patients with SOX10 mutations. ..

More Information


  1. Ilia M, Bazigou E, Price J. Expression of the POU domain transcription factor, Oct-6, is attenuated in the adult mouse telencephalon, but increased by neurotoxic damage. Exp Neurol. 2003;181:159-69 pubmed
    ..The OCT-6 protein, somewhat surprisingly, is found to be cytoplasmic as well as nuclear in certain neuronal subpopulations. Finally, we report that neurotoxic doses of anticonvulsants reactivate OCT-6 expression in adult mouse brain. ..
  2. Andersen B, Weinberg W, Rennekampff O, McEvilly R, Bermingham J, Hooshmand F, et al. Functions of the POU domain genes Skn-1a/i and Tst-1/Oct-6/SCIP in epidermal differentiation. Genes Dev. 1997;11:1873-84 pubmed
    ..on investigation of the role of the POU domain genes Skin-1a/i (Skn-1a/i/Epoc/Oct-11) and Testes-1 (Tst-1/Oct-6/SCIP) in epidermis where proliferating basal keratinocytes withdraw from the cell cycle, migrate suprabasally, and ..
  3. Atanasoski S, Notterpek L, Lee H, Castagner F, Young P, Ehrengruber M, et al. The protooncogene Ski controls Schwann cell proliferation and myelination. Neuron. 2004;43:499-511 pubmed
    ..The myelination-regulating transcription factor Oct6 is involved in a complex modulatory relationship with Ski...
  4. Schreiner S, Cossais F, Fischer K, Scholz S, Bösl M, Holtmann B, et al. Hypomorphic Sox10 alleles reveal novel protein functions and unravel developmental differences in glial lineages. Development. 2007;134:3271-81 pubmed
  5. Kellerer S, Schreiner S, Stolt C, Scholz S, Bösl M, Wegner M. Replacement of the Sox10 transcription factor by Sox8 reveals incomplete functional equivalence. Development. 2006;133:2875-86 pubmed
    ..We conclude that the extent of functional equivalence depends on the tissue and that, despite their relatedness, Sox8 and Sox10 have more unique functions than previously appreciated. ..
  6. Ghazvini M, Mandemakers W, Jaegle M, Piirsoo M, Driegen S, Koutsourakis M, et al. A cell type-specific allele of the POU gene Oct-6 reveals Schwann cell autonomous function in nerve development and regeneration. EMBO J. 2002;21:4612-20 pubmed
    ..Additionally, we show that Krox-20, an important regulatory target of Oct-6 in Schwann cells, is activated, with delayed kinetics, through an Oct-6-independent mechanism in these mice. ..
  7. McEvilly R, de Diaz M, Schonemann M, Hooshmand F, Rosenfeld M. Transcriptional regulation of cortical neuron migration by POU domain factors. Science. 2002;295:1528-32 pubmed
  8. Baranek C, Sock E, Wegner M. The POU protein Oct-6 is a nucleocytoplasmic shuttling protein. Nucleic Acids Res. 2005;33:6277-86 pubmed
    ..Importantly, the nuclear export signal identified for Oct-6 is conserved in most, if not all other vertebrate POU proteins. Nuclear export might therefore be of general relevance for POU protein function throughout development. ..
  9. Mandemakers W, Zwart R, Jaegle M, Walbeehm E, Visser P, Grosveld F, et al. A distal Schwann cell-specific enhancer mediates axonal regulation of the Oct-6 transcription factor during peripheral nerve development and regeneration. EMBO J. 2000;19:2992-3003 pubmed
    ..Thus, our isolation and characterization of the Oct-6 SCE provides the first description of a cis-acting genetic element that responds to converging signalling pathways to drive myelination in the peripheral nervous system. ..
  10. Kuhlbrodt K, Herbarth B, Sock E, Enderich J, Hermans Borgmeyer I, Wegner M. Cooperative function of POU proteins and SOX proteins in glial cells. J Biol Chem. 1998;273:16050-7 pubmed
    Glial cells of the oligodendrocyte lineage express several highly related POU proteins including Tst-1/Oct6/SCIP and Brn-1...
  11. Cossais F, Sock E, Hornig J, Schreiner S, Kellerer S, Bösl M, et al. Replacement of mouse Sox10 by the Drosophila ortholog Sox100B provides evidence for co-option of SoxE proteins into vertebrate-specific gene-regulatory networks through altered expression. Dev Biol. 2010;341:267-81 pubmed publisher
  12. He X, Treacy M, Simmons D, Ingraham H, Swanson L, Rosenfeld M. Expression of a large family of POU-domain regulatory genes in mammalian brain development. Nature. 1989;340:35-41 pubmed
  13. Meijer D, Graus A, Kraay R, Langeveld A, Mulder M, Grosveld G. The octamer binding factor Oct6: cDNA cloning and expression in early embryonic cells. Nucleic Acids Res. 1990;18:7357-65 pubmed
    We have cloned a cDNA encoding a novel octamer binding factor Oct6 that is expressed in undifferentiated ES cells. Expression of the Oct6 gene is downregulated upon differentiation of these cells by aggregate formation...
  14. Friedrich R, Schlierf B, Tamm E, Bösl M, Wegner M. The class III POU domain protein Brn-1 can fully replace the related Oct-6 during schwann cell development and myelination. Mol Cell Biol. 2005;25:1821-9 pubmed
    ..Similar to Oct-6, Brn-1 down-regulated its own expression at later stages of myelination. Thus, class III POU domain proteins can fully replace each other in Schwann cell development. ..
  15. Francius C, Clotman F. Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development. Neuroscience. 2010;165:116-29 pubmed publisher
    ..Together, our data unveil a complex and dynamic expression profile of the OC proteins in spinal MN, which suggests that these factors may participate in regulatory networks that control different steps of motor neuron development. ..
  16. Coulpier F, Decker L, Funalot B, Vallat J, Garcia Bragado F, Charnay P, et al. CNS/PNS boundary transgression by central glia in the absence of Schwann cells or Krox20/Egr2 function. J Neurosci. 2010;30:5958-67 pubmed publisher
    ..This indicates that transgression of the CNS/PNS boundary by central glia can occur in pathological situations in humans and suggests that the underlying mechanisms are common with the mouse. ..
  17. Yu W, Yu H, Chen Z, Strickland S. Disruption of laminin in the peripheral nervous system impedes nonmyelinating Schwann cell development and impairs nociceptive sensory function. Glia. 2009;57:850-9 pubmed publisher
    ..These results show that laminin participates in nonmyelinating SC development and Remak bundle formation and suggest a possible role for laminin deficiency in peripheral sensory neuropathies. ..
  18. Castro D, Skowronska Krawczyk D, Armant O, Donaldson I, Parras C, Hunt C, et al. Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif. Dev Cell. 2006;11:831-44 pubmed
    ..We thus propose that Mash1 synergizes with Brn factors to regulate multiple steps of neurogenesis. ..
  19. McKee K, Yang D, Patel R, Chen Z, Strickland S, Takagi J, et al. Schwann cell myelination requires integration of laminin activities. J Cell Sci. 2012;125:4609-19 pubmed publisher
  20. Iwafuchi Doi M, Matsuda K, Murakami K, Niwa H, Tesar P, Aruga J, et al. Transcriptional regulatory networks in epiblast cells and during anterior neural plate development as modeled in epiblast stem cells. Development. 2012;139:3926-37 pubmed publisher
    ..The direct interaction of these factors with enhancers of Otx2, Hesx1 and Sox2 genes was demonstrated. Thus, a combination of regulatory processes that suppresses non-ANP lineages and promotes neural plate development determines the ANP...
  21. Koizumi H, Tanaka T, Gleeson J. Doublecortin-like kinase functions with doublecortin to mediate fiber tract decussation and neuronal migration. Neuron. 2006;49:55-66 pubmed
    ..Surprisingly, RNAi-mediated knockdown of either gene results in similar migration defects. These results indicate the Dcx microtubule-associated protein family is required for proper neuronal migration and axonal wiring. ..
  22. Hoser M, Potzner M, Koch J, Bösl M, Wegner M, Sock E. Sox12 deletion in the mouse reveals nonreciprocal redundancy with the related Sox4 and Sox11 transcription factors. Mol Cell Biol. 2008;28:4675-87 pubmed publisher
    ..Because of differences in expression levels and transactivation rates, however, functional compensation is not reciprocal. ..
  23. Monk K, Oshima K, Jörs S, Heller S, Talbot W. Gpr126 is essential for peripheral nerve development and myelination in mammals. Development. 2011;138:2673-80 pubmed publisher
    ..hypomyelinating peripheral neuropathy in mice, and expression of differentiated Schwann cell markers, including Pou3f1, Egr2, myelin protein zero and myelin basic protein, is reduced...
  24. Okada A, Kushima K, Aoki Y, Bialer M, Fujiwara M. Identification of early-responsive genes correlated to valproic acid-induced neural tube defects in mice. Birth Defects Res A Clin Mol Teratol. 2005;73:229-38 pubmed
    ..Annotation analysis revealed that the increased genes included gadd45b, ier5, per1, phfl3, pou3f1, and sox4, and the decreased genes included ccne2, ccnl, gas5, egr2, sirt1, and zfp105...
  25. Tole S, Christian C, Grove E. Early specification and autonomous development of cortical fields in the mouse hippocampus. Development. 1997;124:4959-70 pubmed
    ..We suggest that the sources of signals that specify hippocampal field identity lie close to the hippocampal poles, and that the signals operate first on cells at the poles, then move inwards. ..
  26. Dominov J, Miller J. POU homeodomain genes and myogenesis. Dev Genet. 1996;19:108-18 pubmed
  27. Schuurmans C, Armant O, Nieto M, Stenman J, Britz O, Klenin N, et al. Sequential phases of cortical specification involve Neurogenin-dependent and -independent pathways. EMBO J. 2004;23:2892-902 pubmed
    ..Our study thus reveals an unanticipated heterogeneity in the genetic mechanisms specifying the identity of neocortical projection neurons. ..
  28. Louvi A, Nishimura S, Gunel M. Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration. Development. 2014;141:1404-15 pubmed publisher
    ..Thus, we identify a novel cytoplasmic regulator of neuronal migration and demonstrate that its inactivation in radial glia progenitors and nascent neurons produces severe malformations of cortical development. ..
  29. Fotaki V, Larralde O, Zeng S, McLaughlin D, Nichols J, Price D, et al. Loss of Wnt8b has no overt effect on hippocampus development but leads to altered Wnt gene expression levels in dorsomedial telencephalon. Dev Dyn. 2010;239:284-296 pubmed publisher
    ..Thus, loss of Wnt8b does not give rise to an overt morphological phenotype, but does affect expression levels of other Wnts in developing forebrain. ..
  30. LaCombe J, Hanley O, Jung H, Philippidou P, Sürmeli G, Grinstein J, et al. Genetic and functional modularity of Hox activities in the specification of limb-innervating motor neurons. PLoS Genet. 2013;9:e1003184 pubmed publisher
  31. Mallamaci A, Mercurio S, Muzio L, Cecchi C, Pardini C, Gruss P, et al. The lack of Emx2 causes impairment of Reelin signaling and defects of neuronal migration in the developing cerebral cortex. J Neurosci. 2000;20:1109-18 pubmed
    ..In addition, restricted defects along the rostrocaudal and the mediolateral axes are present in the subplate, suggesting an Emx2-specific role in priming the proper development of this layer. ..
  32. Espinosa Medina I, Outin E, Picard C, Chettouh Z, Dymecki S, Consalez G, et al. Neurodevelopment. Parasympathetic ganglia derive from Schwann cell precursors. Science. 2014;345:87-90 pubmed publisher
    ..Thus, cranial Schwann cell precursors are the source of parasympathetic neurons during normal development. ..
  33. Sussel L, Marin O, Kimura S, Rubenstein J. Loss of Nkx2.1 homeobox gene function results in a ventral to dorsal molecular respecification within the basal telencephalon: evidence for a transformation of the pallidum into the striatum. Development. 1999;126:3359-70 pubmed
    ..We present evidence that these phenotypes result from a ventral-to-dorsal transformation of the pallidal primordium into a striatal-like anlage. ..
  34. Toresson H, Potter S, Campbell K. Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for Pax6 and Gsh2. Development. 2000;127:4361-71 pubmed
  35. Bulchand S, Grove E, Porter F, Tole S. LIM-homeodomain gene Lhx2 regulates the formation of the cortical hem. Mech Dev. 2001;100:165-75 pubmed
    ..The defect in the Lhx2-/- telencephalon appears to be at this step. ..
  36. Monuki E, Kuhn R, Weinmaster G, Trapp B, Lemke G. Expression and activity of the POU transcription factor SCIP. Science. 1990;249:1300-3 pubmed
    ..Evidence is provided here for an alternative mode of action. The primary structure of SCIP, a POU protein expressed by developing Schwann cells of the peripheral nervous system, was deduced and SCIP ..
  37. Remedios R, Huilgol D, Saha B, Hari P, Bhatnagar L, Kowalczyk T, et al. A stream of cells migrating from the caudal telencephalon reveals a link between the amygdala and neocortex. Nat Neurosci. 2007;10:1141-50 pubmed
    ..This is first evidence of a dorsal pallial contribution to the amygdala, demonstrating a developmental and mechanistic link between the amygdala and the neocortex. ..
  38. Schwarz D, Varum S, Zemke M, Schöler A, Baggiolini A, Draganova K, et al. Ezh2 is required for neural crest-derived cartilage and bone formation. Development. 2014;141:867-77 pubmed publisher
    ..Our data indicate that craniofacial skeleton formation in higher vertebrates is crucially dependent on epigenetic regulation that keeps in check inhibitors of an osteochondrogenic differentiation program. ..
  39. Nishino J, Saunders T, Sagane K, Morrison S. Lgi4 promotes the proliferation and differentiation of glial lineage cells throughout the developing peripheral nervous system. J Neurosci. 2010;30:15228-40 pubmed publisher
    ..Our results identify a new mechanism regulating enteric gliogenesis as well as novel functions for Lgi4 regulating the proliferation and maturation of glial lineage cells throughout the PNS. ..
  40. Acampora D, Omodei D, Petrosino G, Garofalo A, Savarese M, Nigro V, et al. Loss of the Otx2-Binding Site in the Nanog Promoter Affects the Integrity of Embryonic Stem Cell Subtypes and Specification of Inner Cell Mass-Derived Epiblast. Cell Rep. 2016;15:2651-64 pubmed publisher
    ..Otx2-mediated Nanog regulation thus contributes to the integrity of the ESC state and cell lineage specification in preimplantation development. ..
  41. Hofmann E, Reichart U, Gausterer C, Guelly C, Meijer D, Muller M, et al. Octamer-binding factor 6 (Oct-6/Pou3f1) is induced by interferon and contributes to dsRNA-mediated transcriptional responses. BMC Cell Biol. 2010;11:61 pubmed publisher
    Octamer-binding factor 6 (Oct-6, Pou3f1, SCIP, Tst-1) is a transcription factor of the Pit-Oct-Unc (POU) family. POU proteins regulate key developmental processes and have been identified from a diverse range of species...
  42. Hebert J, Lin M, Partanen J, Rossant J, McConnell S. FGF signaling through FGFR1 is required for olfactory bulb morphogenesis. Development. 2003;130:1101-11 pubmed
    ..Together the results demonstrate an essential role for Fgfr1 in patterning and morphogenesis of the telencephalon. ..
  43. Anderson S, Qiu M, Bulfone A, Eisenstat D, Meneses J, Pedersen R, et al. Mutations of the homeobox genes Dlx-1 and Dlx-2 disrupt the striatal subventricular zone and differentiation of late born striatal neurons. Neuron. 1997;19:27-37 pubmed
    ..Several lines of evidence suggest that mutations in Dlx-1 and Dlx-2 produce abnormalities in the development of the striatal subventricular zone and in the differentiation of striatal matrix neurons. ..
  44. Zimmerman E, Jones C, Fet V, Hogan B, Magnuson M. Nucleotide sequence of mouse SCIP cDNA, a POU-domain transcription factor. Nucleic Acids Res. 1991;19:956 pubmed
  45. Miquelajauregui A, Van de Putte T, Polyakov A, Nityanandam A, Boppana S, Seuntjens E, et al. Smad-interacting protein-1 (Zfhx1b) acts upstream of Wnt signaling in the mouse hippocampus and controls its formation. Proc Natl Acad Sci U S A. 2007;104:12919-24 pubmed
    ..Sip1 is therefore essential to the development of the hippocampus and dentate gyrus, and is able to modulate Wnt signaling in these regions. ..
  46. Li L, Liu C, Biechele S, Zhu Q, Song L, Lanner F, et al. Location of transient ectodermal progenitor potential in mouse development. Development. 2013;140:4533-43 pubmed publisher
    ..Our work not only improves our understanding of ectodermal layer development in early embryos, but also provides a framework for regenerative differentiation towards ectodermal tissues. ..
  47. Rohdewohld H, Gruss P. The gene for the POU domain transcription factor Oct-6 maps to the distal end of mouse chromosome 4. Mamm Genome. 1992;3:119-21 pubmed
  48. Malik K, Jaffe H, Brady J, Young W. The class III POU factor Brn-4 interacts with other class III POU factors and the heterogeneous nuclear ribonucleoprotein U. Brain Res Mol Brain Res. 1997;45:99-107 pubmed
    ..Presumably, these POU proteins (Brain-1, Brain-2, Brain-4 and SCIP) serve as transcriptional transactivators...
  49. Shinozaki K, Miyagi T, Yoshida M, Miyata T, Ogawa M, Aizawa S, et al. Absence of Cajal-Retzius cells and subplate neurons associated with defects of tangential cell migration from ganglionic eminence in Emx1/2 double mutant cerebral cortex. Development. 2002;129:3479-92 pubmed
  50. Xu Q, Wonders C, Anderson S. Sonic hedgehog maintains the identity of cortical interneuron progenitors in the ventral telencephalon. Development. 2005;132:4987-98 pubmed
    ..These results combine in vitro and ex vivo analyses to link embryonic abnormalities in Shh signaling to postnatal alterations in cortical interneuron composition. ..
  51. Garel S, Marin F, Grosschedl R, Charnay P. Ebf1 controls early cell differentiation in the embryonic striatum. Development. 1999;126:5285-94 pubmed
  52. Inoue T, Ogawa M, Mikoshiba K, Aruga J. Zic deficiency in the cortical marginal zone and meninges results in cortical lamination defects resembling those in type II lissencephaly. J Neurosci. 2008;28:4712-25 pubmed publisher
    ..These findings indicate that the Zic genes play critical roles in cortical development through regulating the proliferation of meningeal cells and the pial BM assembly. ..
  53. Caronia Brown G, Yoshida M, Gulden F, Assimacopoulos S, Grove E. The cortical hem regulates the size and patterning of neocortex. Development. 2014;141:2855-65 pubmed publisher
    ..Our findings reveal a much broader role for the hem in cortical development than previously recognized, and emphasize that two major signaling centers interact antagonistically to pattern cerebral cortex. ..