Gene Symbol: Poli
Description: polymerase (DNA directed), iota
Alias: Rad30b, DNA polymerase iota, RAD30 homolog B
Species: mouse
Products:     Poli

Top Publications

  1. McDonald J, Rapic Otrin V, Epstein J, Broughton B, Wang X, Lehmann A, et al. Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta. Genomics. 1999;60:20-30 pubmed
    ..We report here the cloning and localization of a second human homolog of RAD30. Interestingly, RAD30B is localized on chromosome 18q21.1 in a region that is often implicated in the etiology of many human cancers...
  2. McDonald J, Frank E, Plosky B, Rogozin I, Masutani C, Hanaoka F, et al. 129-derived strains of mice are deficient in DNA polymerase iota and have normal immunoglobulin hypermutation. J Exp Med. 2003;198:635-43 pubmed
    Recent studies suggest that DNA polymerase eta (poleta) and DNA polymerase iota (poliota) are involved in somatic hypermutation of immunoglobulin variable genes...
  3. Yuan B, You C, Andersen N, Jiang Y, Moriya M, O Connor T, et al. The roles of DNA polymerases ? and ? in the error-free bypass of N2-carboxyalkyl-2'-deoxyguanosine lesions in mammalian cells. J Biol Chem. 2011;286:17503-11 pubmed publisher
    ..Herein, we used shuttle vector technology and demonstrated that deficiency in Polk or Poli in mouse embryonic fibroblast (MEF) cells resulted in elevated frequencies of G?T and G?A mutations at N(2)-(1-..
  4. Dumstorf C, Clark A, Lin Q, Kissling G, Yuan T, Kucherlapati R, et al. Participation of mouse DNA polymerase iota in strand-biased mutagenic bypass of UV photoproducts and suppression of skin cancer. Proc Natl Acad Sci U S A. 2006;103:18083-8 pubmed
    b>DNA polymerase iota (pol iota) is a conserved Y family enzyme that is implicated in translesion DNA synthesis (TLS) but whose cellular functions remain uncertain...
  5. Lee G, Nishimori H, Sasaki Y, Matsushita H, Kitagawa T, Tokino T. Analysis of lung tumorigenesis in chimeric mice indicates the Pulmonary adenoma resistance 2 (Par2) locus to operate in the tumor-initiation stage in a cell-autonomous manner: detection of polymorphisms in the Poli gene as a candidate for Par2. Oncogene. 2003;22:2374-82 pubmed
    ..3 Mbp segment containing three known genes, Poli, Mbd2 and Dcc...
  6. Wang M, Devereux T, Vikis H, McCulloch S, Holliday W, Anna C, et al. Pol iota is a candidate for the mouse pulmonary adenoma resistance 2 locus, a major modifier of chemically induced lung neoplasia. Cancer Res. 2004;64:1924-31 pubmed
    ..at this moment due to the recent database updating, we have found that the Pol iota gene, encoding the DNA polymerase iota, contains 25 nucleotide polymorphisms in its coding region between A/J and BALB/cJ mice, resulting in a ..
  7. Maul R, MacCarthy T, Frank E, Donigan K, McLenigan M, Yang W, et al. DNA polymerase ? functions in the generation of tandem mutations during somatic hypermutation of antibody genes. J Exp Med. 2016;213:1675-83 pubmed publisher
    ..The results support a model where Pol ? occasionally accesses the replication fork to generate a first mutation, and Pol ? extends the mismatch with a second mutation. ..
  8. Lee G, Matsushita H. Genetic linkage between Pol iota deficiency and increased susceptibility to lung tumors in mice. Cancer Sci. 2005;96:256-9 pubmed
    ..The result indicates a possibility that the Pol iota gene may participate in error-free repair of damaged DNA and prevention of lung tumor development. ..
  9. Martomo S, Yang W, Vaisman A, Maas A, Yokoi M, Hoeijmakers J, et al. Normal hypermutation in antibody genes from congenic mice defective for DNA polymerase iota. DNA Repair (Amst). 2006;5:392-8 pubmed
    ..polymerase iota, hypermutation was normal in the 129 strain of mice, which has a natural nonsense mutation in the Poli gene...

More Information


  1. Burschowsky D, Rudolf F, Rabut G, Herrmann T, Peter M, Matthias P, et al. Structural analysis of the conserved ubiquitin-binding motifs (UBMs) of the translesion polymerase iota in complex with ubiquitin. J Biol Chem. 2011;286:1364-73 pubmed publisher
    ..Indeed, yeast cells expressing ubiquitin mutants specifically defective for UBM binding are viable but sensitive to DNA damaging conditions that require TLS for repair. ..
  2. Velasco Miguel S, Richardson J, Gerlach V, Lai W, Gao T, Russell L, et al. Constitutive and regulated expression of the mouse Dinb (Polkappa) gene encoding DNA polymerase kappa. DNA Repair (Amst). 2003;2:91-106 pubmed
    ..The mouse (but not the human) Polkappa gene is primarily regulated by the p53 gene and is upregulated in response to exposure to various DNA-damaging agents in a p53-dependent manner. ..
  3. Frank E, McDonald J, Yang W, Woodgate R. Mouse DNA polymerase ι lacking the forty-two amino acids encoded by exon-2 is catalytically inactive in vitro. DNA Repair (Amst). 2017;50:71-76 pubmed publisher
    ..2003, we reported that 129-derived strains of mice carry a naturally occurring nonsense mutation at codon 27 of the Poli gene that would produce a polι peptide of just 26 amino acids, rather then the full-length 717 amino acid wild-..
  4. Aoufouchi S, De Smet A, Delbos F, Gelot C, Guerrera I, Weill J, et al. 129-Derived Mouse Strains Express an Unstable but Catalytically Active DNA Polymerase Iota Variant. Mol Cell Biol. 2015;35:3059-70 pubmed publisher
  5. Suzuki N, Hiraki M, Yamada Y, Matsugaki N, Igarashi N, Kato R, et al. Crystallization of small proteins assisted by green fluorescent protein. Acta Crystallogr D Biol Crystallogr. 2010;66:1059-66 pubmed publisher
    ..Therefore, it is likely that GFP can assist in the crystallization of small proteins and of protein domains in general. ..
  6. Ohkumo T, Kondo Y, Yokoi M, Tsukamoto T, Yamada A, Sugimoto T, et al. UV-B radiation induces epithelial tumors in mice lacking DNA polymerase eta and mesenchymal tumors in mice deficient for DNA polymerase iota. Mol Cell Biol. 2006;26:7696-706 pubmed
    ..Another translesion synthesis polymerase, Pol iota, a Pol eta paralog encoded by the Poli gene, is naturally deficient in the 129 mouse strain, and the function of Pol iota is enigmatic...
  7. Delbos F, De Smet A, Faili A, Aoufouchi S, Weill J, Reynaud C. Contribution of DNA polymerase eta to immunoglobulin gene hypermutation in the mouse. J Exp Med. 2005;201:1191-6 pubmed
    ..Breeding these mice with animals harboring the stop codon mutation of the 129/Olain background in their DNA polymerase iota gene did not alter this pattern further...
  8. Shimizu T, Azuma T, Ishiguro M, Kanjo N, Yamada S, Ohmori H. Normal immunoglobulin gene somatic hypermutation in Pol kappa-Pol iota double-deficient mice. Immunol Lett. 2005;98:259-64 pubmed
    ..We found that the double-deficient mice had the normal SHM frequency and profile, rendering them indistinguishable from Polkappa-deficient mice and thus conclude that Poliota and Polkappa are dispensable for SHM in mice. ..
  9. Iguchi M, Osanai M, Hayashi Y, Koentgen F, Lee G. The error-prone DNA polymerase ? provides quantitative resistance to lung tumorigenesis and mutagenesis in mice. Oncogene. 2014;33:3612-7 pubmed publisher
  10. Kanao R, Yokoi M, Ohkumo T, Sakurai Y, Dotsu K, Kura S, et al. UV-induced mutations in epidermal cells of mice defective in DNA polymerase η and/or ι. DNA Repair (Amst). 2015;29:139-46 pubmed publisher
  11. Wang M, Wang Y, You M, Devereux T. Analysis of the Par2 modifier of pulmonary adenoma formation in mice. Exp Lung Res. 2005;31:193-204 pubmed
    ..The authors recently conducted studies to positionally clone the Par2 gene. This review summarizes the effort and progress towards the identification of Par2 candidates. ..
  12. Guo C, Fischhaber P, Luk Paszyc M, Masuda Y, Zhou J, Kamiya K, et al. Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. EMBO J. 2003;22:6621-30 pubmed
    ..Our observations suggest that Rev1 plays a role(s) in mediating protein-protein interactions among DNA polymerases required for TLS. The precise function(s) of these interactions during TLS remains to be determined. ..
  13. Kazakov A, Grishina E, Tarantul V, Gening L. Effect of human cell malignancy on activity of DNA polymerase iota. Biochemistry (Mosc). 2010;75:905-11 pubmed
    ..To clarify the possible role of incorrect DNA polymerase iota (Pol iota) function in increased frequency of mutations in mammalian cells, the activity of this enzyme in ..
  14. Bienko M, Green C, Crosetto N, Rudolf F, Zapart G, Coull B, et al. Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis. Science. 2005;310:1821-4 pubmed
    ..Our results indicate that Ub-binding domains of Y-family polymerases play crucial regulatory roles in TLS...
  15. Kazachenko K, Miropolskaya N, Gening L, Tarantul V, Makarova A. Alternative splicing at exon 2 results in the loss of the catalytic activity of mouse DNA polymerase iota in vitro. DNA Repair (Amst). 2017;50:77-82 pubmed publisher
    Y-family DNA polymerase iota (Pol ?) possesses both DNA polymerase and dRP lyase activities and was suggested to be involved in DNA translesion synthesis and base excision repair in mammals...