Gene Symbol: Pld6
Description: phospholipase D family, member 6
Alias: 4933433K01Rik, Gm10, Zuc, mZuc, mitoPLD, mitochondrial cardiolipin hydrolase, choline phosphatase 6, mitochondrial phospholipase, novel Phospholipase D Active site motif-containing protein, phosphatidylcholine-hydrolyzing phospholipase D6, phospholipase D6, protein zucchini homolog
Species: mouse
Products:     Pld6

Top Publications

  1. Watanabe T, Chuma S, Yamamoto Y, Kuramochi Miyagawa S, Totoki Y, Toyoda A, et al. MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline. Dev Cell. 2011;20:364-75 pubmed publisher
    b>MITOPLD is a member of the phospholipase D superfamily proteins conserved among diverse species. Zucchini (Zuc), the Drosophila homolog of MITOPLD, has been implicated in primary biogenesis of Piwi-interacting RNAs (piRNAs)...
  2. Huang H, Gao Q, Peng X, Choi S, Sarma K, Ren H, et al. piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling. Dev Cell. 2011;20:376-87 pubmed publisher
    The mammalian Phospholipase D MitoPLD facilitates mitochondrial fusion by generating the signaling lipid phosphatidic acid (PA)...
  3. Ipsaro J, Haase A, Knott S, Joshua Tor L, Hannon G. The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis. Nature. 2012;491:279-83 pubmed publisher
    ..Here we produced a dimeric, soluble fragment of the mouse Zucchini homologue (mZuc; also known as PLD6) and show that it possesses single-strand-specific nuclease activity. A crystal structure of mZuc at 1...
  4. Adachi Y, Itoh K, Yamada T, Cerveny K, Suzuki T, Macdonald P, et al. Coincident Phosphatidic Acid Interaction Restrains Drp1 in Mitochondrial Division. Mol Cell. 2016;63:1034-43 pubmed publisher
    ..Moreover, a PA-producing phospholipase, MitoPLD, binds Drp1, creating a PA-rich microenvironment in the vicinity of a division apparatus...
  5. Inoue K, Ichiyanagi K, Fukuda K, Glinka M, Sasaki H. Switching of dominant retrotransposon silencing strategies from posttranscriptional to transcriptional mechanisms during male germ-cell development in mice. PLoS Genet. 2017;13:e1006926 pubmed publisher
    ..studies of DNA methylation and polyA(+) RNAs (transcriptome) in developing male germ cells from Pld6/Mitopld and Dnmt3l knockout mice, which are defective in piRNA biogenesis and de novo DNA methylation, respectively...
  6. Watanabe T, Tomizawa S, Mitsuya K, Totoki Y, Yamamoto Y, Kuramochi Miyagawa S, et al. Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus. Science. 2011;332:848-52 pubmed publisher
    ..A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1. ..
  7. Gao Q, Frohman M. Roles for the lipid-signaling enzyme MitoPLD in mitochondrial dynamics, piRNA biogenesis, and spermatogenesis. BMB Rep. 2012;45:7-13 pubmed
    ..A fascinating member of the family, MitoPLD, is anchored to the mitochondrial surface and has two reported roles...
  8. Nishimasu H, Ishizu H, Saito K, Fukuhara S, Kamatani M, Bonnefond L, et al. Structure and function of Zucchini endoribonuclease in piRNA biogenesis. Nature. 2012;491:284-7 pubmed publisher
    ..Here we show that Zucchini (Zuc), a mitochondrial phospholipase D (PLD) superfamily member, is an endoribonuclease essential for primary piRNA biogenesis...
  9. Vourekas A, Zheng K, Fu Q, Maragkakis M, Alexiou P, Ma J, et al. The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing. Genes Dev. 2015;29:617-29 pubmed publisher
    ..Our results support a model in which MOV10L1 RNA helicase activity promotes unwinding and funneling of the single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing. ..

More Information


  1. Zhang Y, Liu X, Bai J, Tian X, Zhao X, Liu W, et al. Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis. Mol Cell. 2016;61:111-24 pubmed publisher
    ..MIGA proteins function downstream of mitofusin and interact with MitoPLD to stabilize MitoPLD and facilitate MitoPLD dimer formation...
  2. Kabayama Y, Toh H, Katanaya A, Sakurai T, Chuma S, Kuramochi Miyagawa S, et al. Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes. Nucleic Acids Res. 2017;45:5387-5398 pubmed publisher
    ..MIWI (or PIWIL1) depletion had a smaller effect. In oocytes lacking PLD6 (or ZUCCHINI or MITOPLD), a mitochondrial nuclease/phospholipase involved in piRNA biogenesis in male germ cells, the piRNA level was ..