Pemt

Summary

Gene Symbol: Pemt
Description: phosphatidylethanolamine N-methyltransferase
Alias: PEAMT, PEMT2, PLMT, Pempt, Pempt2, phosphatidylethanolamine N-methyltransferase, phospholipid methyltransferase
Species: mouse
Products:     Pemt

Top Publications

  1. Vance D. Physiological roles of phosphatidylethanolamine N-methyltransferase. Biochim Biophys Acta. 2013;1831:626-32 pubmed publisher
    Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes the methylation of phosphatidylethanolamine to phosphatidylcholine (PC). This 22...
  2. Zhao Y, Su B, Jacobs R, Kennedy B, Francis G, Waddington E, et al. Lack of phosphatidylethanolamine N-methyltransferase alters plasma VLDL phospholipids and attenuates atherosclerosis in mice. Arterioscler Thromb Vasc Biol. 2009;29:1349-55 pubmed publisher
    ..We, therefore, tested the hypothesis that lack of phosphatidylethanolamine N-methyltransferase (PEMT), a hepatic enzyme catalyzing PC biosynthesis, attenuates the development of atherosclerosis...
  3. Jacobs R, Zhao Y, Koonen D, Sletten T, Su B, Lingrell S, et al. Impaired de novo choline synthesis explains why phosphatidylethanolamine N-methyltransferase-deficient mice are protected from diet-induced obesity. J Biol Chem. 2010;285:22403-13 pubmed publisher
    ..the sequential methylation of phosphatidylethanolamine, catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). The current study investigates whether or not hepatic PC biosynthesis is linked to diet-induced obesity...
  4. Noga A, Vance D. A gender-specific role for phosphatidylethanolamine N-methyltransferase-derived phosphatidylcholine in the regulation of plasma high density and very low density lipoproteins in mice. J Biol Chem. 2003;278:21851-9 pubmed
    Phosphatidylethanolamine N-methyltransferase (PEMT)is involved in a secondary pathway for production of phosphatidylcholine (PC) in liver...
  5. Walkey C, Donohue L, Bronson R, Agellon L, Vance D. Disruption of the murine gene encoding phosphatidylethanolamine N-methyltransferase. Proc Natl Acad Sci U S A. 1997;94:12880-5 pubmed
    ..We investigated the function of PEMT and its role in animal physiology by targeted disruption of its gene, Pempt2. A targeting vector that interrupts exon 2 was constructed and introduced into mice yielding three genotypes: ..
  6. Wang L, Magdaleno S, Tabas I, Jackowski S. Early embryonic lethality in mice with targeted deletion of the CTP:phosphocholine cytidylyltransferase alpha gene (Pcyt1a). Mol Cell Biol. 2005;25:3357-63 pubmed
    ..These data show that CCTalpha expression is required for early embryonic development, but that a 50% reduction in enzyme activity has little detectable impact on the operation of the CDP-choline metabolic pathway in adult tissues. ..
  7. Zhu X, Mar M, Song J, Zeisel S. Deletion of the Pemt gene increases progenitor cell mitosis, DNA and protein methylation and decreases calretinin expression in embryonic day 17 mouse hippocampus. Brain Res Dev Brain Res. 2004;149:121-9 pubmed
    ..derived from the diet as well as from de novo synthesis catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT)...
  8. Noga A, Stead L, Zhao Y, Brosnan M, Brosnan J, Vance D. Plasma homocysteine is regulated by phospholipid methylation. J Biol Chem. 2003;278:5952-5 pubmed
    ..Thus, phosphatidylethanolamine N-methyltransferase is an important source of plasma homocysteine and a potential therapeutic target for hyperhomocysteinemia. ..
  9. Resseguie M, Song J, Niculescu M, da Costa K, Randall T, Zeisel S. Phosphatidylethanolamine N-methyltransferase (PEMT) gene expression is induced by estrogen in human and mouse primary hepatocytes. FASEB J. 2007;21:2622-32 pubmed
    ..of the requirement can be met by endogenous synthesis catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). Premenopausal women are relatively resistant to choline deficiency compared with postmenopausal women and men...

More Information

Publications44

  1. Gao X, van der Veen J, Vance J, Thiesen A, Vance D, Jacobs R. Lack of phosphatidylethanolamine N-methyltransferase alters hepatic phospholipid composition and induces endoplasmic reticulum stress. Biochim Biophys Acta. 2015;1852:2689-99 pubmed publisher
    ..Phosphatidylethanolamine N-methyltransferase (PEMT) is a hepatic enzyme located on the ER and mitochondria-associated membranes and catalyzes phosphatidylcholine (PC) ..
  2. Jacobs R, Jiang H, Kennelly J, Orlicky D, Allen R, Stabler S, et al. Cystathionine beta-synthase deficiency alters hepatic phospholipid and choline metabolism: Post-translational repression of phosphatidylethanolamine N-methyltransferase is a consequence rather than a cause of liver injury in homocystinuria. Mol Genet Metab. 2017;120:325-336 pubmed publisher
    ..PL) metabolism by disruption of both the Kennedy pathway and phosphatidylethanolamine N-methyltransferase (PEMT) mediated synthesis of phosphatidylcholine (PC)...
  3. Cole L, Dolinsky V, Dyck J, Vance D. Impaired phosphatidylcholine biosynthesis reduces atherosclerosis and prevents lipotoxic cardiac dysfunction in ApoE-/- Mice. Circ Res. 2011;108:686-94 pubmed publisher
    ..approximately one-third of hepatic PC can also be synthesized by phosphatidylethanolamine N-methyltransferase (PEMT). PEMT is required for normal secretion of very-low-density lipoproteins from the liver...
  4. Robichaud J, Francis G, Vance D. A role for hepatic scavenger receptor class B, type I in decreasing high density lipoprotein levels in mice that lack phosphatidylethanolamine N-methyltransferase. J Biol Chem. 2008;283:35496-506 pubmed publisher
    Phosphatidylethanolamine N-methyltransferase (PEMT) is a liver-specific enzyme that converts phosphatidylethanolamine to phosphatidylcholine (PC)...
  5. van der Veen J, Lingrell S, da Silva R, Jacobs R, Vance D. The concentration of phosphatidylethanolamine in mitochondria can modulate ATP production and glucose metabolism in mice. Diabetes. 2014;63:2620-30 pubmed publisher
    Phosphatidylethanolamine (PE) N-methyltransferase (PEMT) catalyzes the synthesis of phosphatidylcholine (PC) in the liver. Mice lacking PEMT are protected against diet-induced obesity and insulin resistance...
  6. Vance D, Li Z, Jacobs R. Hepatic phosphatidylethanolamine N-methyltransferase, unexpected roles in animal biochemistry and physiology. J Biol Chem. 2007;282:33237-41 pubmed
  7. Gao X, van der Veen J, Fernandez Patron C, Vance J, Vance D, Jacobs R. Insufficient glucose supply is linked to hypothermia upon cold exposure in high-fat diet-fed mice lacking PEMT. J Lipid Res. 2015;56:1701-10 pubmed publisher
    Mice that lack phosphatidylethanolamine N-methyltransferase (Pemt(-/-) mice) are protected from high-fat (HF) diet-induced obesity...
  8. Li Z, Agellon L, Vance D. Choline redistribution during adaptation to choline deprivation. J Biol Chem. 2007;282:10283-9 pubmed
    ..in the liver by the methylation of phosphatidylethanolamine by phosphatidylethanolamine N-methyltransferase (PEMT)...
  9. Li Z, Agellon L, Allen T, Umeda M, Jewell L, Mason A, et al. The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis. Cell Metab. 2006;3:321-31 pubmed
    ..PC is synthesized via the choline pathway or by methylation of PE via phosphatidylethanolamine N-methyltransferase (PEMT). Pemt(-/-) mice fed a choline-deficient (CD) diet develop rapid steatohepatitis leading to liver failure...
  10. Waite K, Cabilio N, Vance D. Choline deficiency-induced liver damage is reversible in Pemt(-/-) mice. J Nutr. 2002;132:68-71 pubmed
    ..CDP-choline) pathway and the methylation pathway, which utilizes phosphatidylethanolamine-N-methyltransferase (PEMT). Fatal liver damage occurs in Pemt(-/-)mice fed a choline-deficient (CD) diet...
  11. Verkade H, Havinga R, Shields D, Wolters H, Bloks V, Kuipers F, et al. The phosphatidylethanolamine N-methyltransferase pathway is quantitatively not essential for biliary phosphatidylcholine secretion. J Lipid Res. 2007;48:2058-64 pubmed
    The phosphatidylethanolamine N-methyltransferase (PEMT) pathway of phosphatidylcholine (PC) biosynthesis is not essential for the highly specific acyl chain composition of biliary PC...
  12. Waite K, Vance D. Dimethylethanolamine does not prevent liver failure in phosphatidylethanolamine N-methyltransferase-deficient mice fed a choline-deficient diet. Biochim Biophys Acta. 2004;1636:175-82 pubmed
    Mice that lack phosphatidylethanolamine-N-methyltransferase (PEMT) and are fed a choline-deficient (CD) diet suffer severe liver damage and do not survive...
  13. Noga A, Vance D. Insights into the requirement of phosphatidylcholine synthesis for liver function in mice. J Lipid Res. 2003;44:1998-2005 pubmed
    ..Immunoblot analyses demonstrated that twice as much PEMT2 was present in livers from female compared with male mice...
  14. Zhu X, Song J, Mar M, Edwards L, Zeisel S. Phosphatidylethanolamine N-methyltransferase (PEMT) knockout mice have hepatic steatosis and abnormal hepatic choline metabolite concentrations despite ingesting a recommended dietary intake of choline. Biochem J. 2003;370:987-93 pubmed
    ..methylation of phosphatidylethanolamine catalysed by the enzyme phosphatidylethanolamine N -methyltransferase (PEMT). This is the only known pathway that produces new choline molecules...
  15. Hörl G, Wagner A, Cole L, Malli R, Reicher H, Kotzbeck P, et al. Sequential synthesis and methylation of phosphatidylethanolamine promote lipid droplet biosynthesis and stability in tissue culture and in vivo. J Biol Chem. 2011;286:17338-50 pubmed publisher
    ..Activity and protein of PE N-methyltransferase (PEMT), which produces PC by methylation of PE, are absent in 3T3-L1 fibroblasts but were induced at day 5...
  16. Walkey C, Yu L, Agellon L, Vance D. Biochemical and evolutionary significance of phospholipid methylation. J Biol Chem. 1998;273:27043-6 pubmed
    ..of phosphatidylethanolamine, catalyzed by phosphatidylethanolamine N-methyltransferase and encoded by the Pempt gene...
  17. Watanabe M, Nakatsuka A, Murakami K, Inoue K, Terami T, Higuchi C, et al. Pemt deficiency ameliorates endoplasmic reticulum stress in diabetic nephropathy. PLoS ONE. 2014;9:e92647 pubmed publisher
    Phosphatidylethanolamine N-methyltransferase (Pemt) catalyzes the methylation of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) mainly in the liver...
  18. da Costa K, Rai K, Craciunescu C, Parikh K, Mehedint M, Sanders L, et al. Dietary docosahexaenoic acid supplementation modulates hippocampal development in the Pemt-/- mouse. J Biol Chem. 2010;285:1008-15 pubmed publisher
    ..Phosphatidylethanolamine-N-methyltransferase (PEMT) catalyzes the biosynthesis of phosphatidylcholine from phosphatidylethanolamine enriched in DHA and many humans ..
  19. Li Z, Agellon L, Vance D. The role of phosphatidylethanolamine methyltransferase in a mouse model of intrahepatic cholestasis. Biochim Biophys Acta. 2011;1811:278-83 pubmed publisher
    ..We found that lack of phosphatidylethanolamine N-methyltransferase (PEMT) decreased liver damage in Abcb4(-/-) mice caused by exposure of the liver to excess bile acids...
  20. van der Veen J, Lingrell S, Gao X, Quiroga A, Takawale A, Armstrong E, et al. Pioglitazone attenuates hepatic inflammation and fibrosis in phosphatidylethanolamine N-methyltransferase-deficient mice. Am J Physiol Gastrointest Liver Physiol. 2016;310:G526-38 pubmed publisher
    Phosphatidylethanolamine N-methyltransferase (PEMT) is an important enzyme in hepatic phosphatidylcholine (PC) biosynthesis...
  21. Kulinski A, Rustaeus S, Vance J. Microsomal triacylglycerol transfer protein is required for lumenal accretion of triacylglycerol not associated with ApoB, as well as for ApoB lipidation. J Biol Chem. 2002;277:31516-25 pubmed
  22. Nakatsuka A, Matsuyama M, Yamaguchi S, Katayama A, Eguchi J, Murakami K, et al. Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci Rep. 2016;6:21721 pubmed publisher
    ..Here, we report that the insufficiency of phosphatidylethanolamine N-methyltransferase (PEMT) is a risk for the lean NASH...
  23. Tasseva G, van der Veen J, Lingrell S, Jacobs R, Vance D, Vance J. Lack of phosphatidylethanolamine N-methyltransferase in mice does not promote fatty acid oxidation in skeletal muscle. Biochim Biophys Acta. 2016;1861:119-129 pubmed publisher
    Phosphatidylethanolamine N-methyltransferase (PEMT) converts phosphatidylethanolamine (PE) to phosphatidylcholine (PC) in the liver...
  24. van der Veen J, Lingrell S, Gao X, Takawale A, Kassiri Z, Vance D, et al. Fenofibrate, but not ezetimibe, prevents fatty liver disease in mice lacking phosphatidylethanolamine N-methyltransferase. J Lipid Res. 2017;58:656-667 pubmed publisher
    Mice lacking phosphatidylethanolamine N-methyltransferase (PEMT) are protected from high-fat diet (HFD)-induced obesity and insulin resistance...
  25. Zhu X, Zeisel S. Gene expression profiling in phosphatidylethanolamine N-methyltransferase knockout mice. Brain Res Mol Brain Res. 2005;134:239-55 pubmed
    ..as from de novo methylation of phosphatidylethanolamine catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). Pemt knockout mice have no endogenous synthesis of choline molecules...
  26. Gao X, van der Veen J, Hermansson M, Ordoñez M, Gomez Muñoz A, Vance D, et al. Decreased lipogenesis in white adipose tissue contributes to the resistance to high fat diet-induced obesity in phosphatidylethanolamine N-methyltransferase-deficient mice. Biochim Biophys Acta. 2015;1851:152-62 pubmed publisher
    Mice lacking phosphatidylethanolamine N-methyltransferase (PEMT, Pemt(-/-) mice) are resistant to high-fat diet (HFD)-induced obesity (DIO) but develop non-alcoholic steatohepatitis...
  27. van der Veen J, Lingrell S, Vance D. The membrane lipid phosphatidylcholine is an unexpected source of triacylglycerol in the liver. J Biol Chem. 2012;287:23418-26 pubmed publisher
    ..Thus, lipoprotein-associated PC should be considered a quantitatively significant source of triacylglycerol for the etiology of hepatic steatosis. ..
  28. Walkey C, Cui Z, Agellon L, Vance D. Characterization of the murine phosphatidylethanolamine N-methyltransferase-2 gene. J Lipid Res. 1996;37:2341-50 pubmed
    ..In the present studies, we cloned and characterized the murine gene for PEMT2, the isoform of the enzyme that localized to the mitochondria-associated membrane...
  29. Oki T, Yamazaki K, Kuromitsu J, Okada M, Tanaka I. cDNA cloning and mapping of a novel subtype of glutamine:fructose-6-phosphate amidotransferase (GFAT2) in human and mouse. Genomics. 1999;57:227-34 pubmed
    ..GFAT2 can provide insights into understanding the roles of the hexosamine pathway in various tissues, particularly with the development of glucose toxicity and diabetes complications. ..
  30. Bickert A, Ginkel C, Kol M, Vom Dorp K, Jastrow H, Degen J, et al. Functional characterization of enzymes catalyzing ceramide phosphoethanolamine biosynthesis in mice. J Lipid Res. 2015;56:821-35 pubmed publisher
    ..Our data provide a first inventory of CPE species and CPE-biosynthetic enzymes in mammals. ..
  31. Noga A, Zhao Y, Vance D. An unexpected requirement for phosphatidylethanolamine N-methyltransferase in the secretion of very low density lipoproteins. J Biol Chem. 2002;277:42358-65 pubmed
    Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes the conversion of phosphatidylethanolamine to phosphatidylcholine (PC)...
  32. Watkins S, Zhu X, Zeisel S. Phosphatidylethanolamine-N-methyltransferase activity and dietary choline regulate liver-plasma lipid flux and essential fatty acid metabolism in mice. J Nutr. 2003;133:3386-91 pubmed
    Phosphatidylethanolamine-N-methyltransferase (PEMT) catalyzes the methylation of phosphatidylethanolamine to form phosphatidylcholine (PC) and represents one of the two major pathways for PC biosynthesis...
  33. Wu G, Zhang L, Li T, Zuniga A, Lopaschuk G, Li L, et al. Choline supplementation promotes hepatic insulin resistance in phosphatidylethanolamine N-methyltransferase-deficient mice via increased glucagon action. J Biol Chem. 2013;288:837-47 pubmed publisher
    Biosynthesis of hepatic choline via phosphatidylethanolamine N-methyltransferase (PEMT) plays an important role in the development of type 2 diabetes and obesity...
  34. Cole L, Vance D. A role for Sp1 in transcriptional regulation of phosphatidylethanolamine N-methyltransferase in liver and 3T3-L1 adipocytes. J Biol Chem. 2010;285:11880-91 pubmed publisher
    ..pathway for phosphatidylcholine biosynthesis in liver is catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). We have now identified 3T3-L1 adipocytes as a cell culture model that expresses PEMT endogenously...
  35. Li Z, Agellon L, Vance D. Phosphatidylcholine homeostasis and liver failure. J Biol Chem. 2005;280:37798-802 pubmed
    ..of phosphatidylethanolamine to phosphatidylcholine (PC) by phosphatidylethanolamine N-methyltransferase (PEMT) coupled to PC degradation...