Pcx

Summary

Gene Symbol: Pcx
Description: pyruvate carboxylase
Alias: Pcb, pyruvate carboxylase, mitochondrial, pyruvate decarboxylase, pyruvic carboxylase
Species: mouse
Products:     Pcx

Top Publications

  1. Jitrapakdee S, Slawik M, Medina Gomez G, Campbell M, Wallace J, Sethi J, et al. The peroxisome proliferator-activated receptor-gamma regulates murine pyruvate carboxylase gene expression in vivo and in vitro. J Biol Chem. 2005;280:27466-76 pubmed
    ..Our findings identified PC as a PPARgamma-regulated gene and suggested a role for PPARgamma regulating intermediary metabolism. ..
  2. Yoder J, Litman G. The zebrafish fth1, slc3a2, men1, pc, fgf3 and cycd1 genes define two regions of conserved synteny between linkage group 7 and human chromosome 11q13. Gene. 2000;261:235-42 pubmed
    ..These six genes, along with men1, fgf3 and cycd1 define two regions of conserved synteny between linkage group 7 and 11q13. ..
  3. Freytag S, Collier K. Molecular cloning of a cDNA for human pyruvate carboxylase. Structural relationship to other biotin-containing carboxylases and regulation of mRNA content in differentiating preadipocytes. J Biol Chem. 1984;259:12831-7 pubmed
    ..These data demonstrated that pyruvate carboxylase mRNA content increased 23-fold in 7 days after the onset of differentiation. ..
  4. Bose S, Hutson I, Harris C. Hepatic Glucocorticoid Receptor Plays a Greater Role Than Adipose GR in Metabolic Syndrome Despite Renal Compensation. Endocrinology. 2016;157:4943-4960 pubmed
    ..This indicates that liver GR might be more important in development of MS in dexamethasone-treated mice, whereas adipose GR plays a little role in these paradigms. ..
  5. Mrozowska P, Fukuda M. Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J Cell Biol. 2016;213:355-69 pubmed publisher
    ..Our results give substantial insight into regulation of the transcytosis of this apical marker and highlight differences between trafficking mechanisms in 2D and 3D cell cultures. ..
  6. Park S, Kim S, Choi H, Chang C, Kim E. TR4 promotes fatty acid synthesis in 3T3-L1 adipocytes by activation of pyruvate carboxylase expression. FEBS Lett. 2014;588:3947-53 pubmed publisher
    ..Moreover, TR4 microRNA reduced PC expression with decreased fatty acid synthesis in 3T3-L1 adipocytes, suggesting that TR4-mediated enhancement of fatty acid synthesis in adipocytes requires increased expression of PC gene. ..
  7. De Pauw A, Demine S, Tejerina S, Dieu M, Delaive E, Kel A, et al. Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction. Am J Physiol Endocrinol Metab. 2012;302:E1123-41 pubmed publisher
    ..These results suggest a new mechanism by which the effects of mitochondrial uncoupling might limit triglyceride accumulation in adipocytes. ..
  8. Rojvirat P, Chavalit T, Muangsawat S, Thonpho A, Jitrapakdee S. Functional characterization of the proximal promoter of the murine pyruvate carboxylase gene in hepatocytes: role of multiple gc boxes. Biochim Biophys Acta. 2011;1809:541-8 pubmed publisher
    ..Mutants harboring two disrupted GC boxes showed a further decrease in promoter activity similar to the triple GC box mutant. Neither Sp1 nor Sp3 was able to fully restore the promoter activities of these mutants to that the WT level. ..
  9. Thonpho A, Sereeruk C, Rojvirat P, Jitrapakdee S. Identification of the cyclic AMP responsive element (CRE) that mediates transcriptional regulation of the pyruvate carboxylase gene in HepG2 cells. Biochem Biophys Res Commun. 2010;393:714-9 pubmed publisher
    ..95 kbp and 781 bp upstream of the mouse PC gene. Electrophoretic mobility shifted and chromatin immunoprecipitation assays demonstrated that CREB bind to -1639/-1631 CRE of mouse PC gene in vitro and in vivo, respectively. ..

More Information

Publications19

  1. Han J, Liu Y. Reduction of islet pyruvate carboxylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice. J Endocrinol. 2010;204:143-52 pubmed publisher
    ..Our findings suggest that islet PC might play a role in the development of T2DM where reduction of PC activity might be a consequence of mild hyperglycemia and a cause for severe hyperglycemia. ..
  2. Jitrapakdee S, Petchamphai N, Sunyakumthorn P, Wallace J, Boonsaeng V. Structural and promoter regions of the murine pyruvate carboxylase gene. Biochem Biophys Res Commun. 2001;287:411-7 pubmed
    ..Both transcripts are highly expressed in kidney and liver and moderately expressed in heart and testis and expressed at a low level in spleen. ..
  3. Walker M, Baker E, Wallace J, Sutherland G. Assignment of the human pyruvate carboxylase gene (PC) to 11q13.4 by fluorescence in situ hybridisation. Cytogenet Cell Genet. 1995;69:187-9 pubmed
    ..The gene for pyruvate carboxylase (PC) has been localised by fluorescence in situ hybridisation to normal and FRA11A human chromosomes. PC maps to 11q13 and distal to FRA11A, localising PC to 11q13.4-->q13.5. ..
  4. Fernandes M, Lespinasse F, Rotomondo F, Poirier C, Guenet J, Gaudray P, et al. Comparative mapping of two adjacent regions of MMU19 with their human counterpart on HSA11q13. Cytogenet Cell Genet. 1998;81:237-46 pubmed
    ..5-Mb region. Strikingly, the 0.5-Mb region has a similar size in human but gene order is shuffled. The overall comparative map shows that these two regions are inverted on MMU19 when compared with HSA11q13. ..
  5. Kumashiro N, Beddow S, Vatner D, Majumdar S, Cantley J, Guebre Egziabher F, et al. Targeting pyruvate carboxylase reduces gluconeogenesis and adiposity and improves insulin resistance. Diabetes. 2013;62:2183-94 pubmed publisher
    ..Tissue-specific inhibition of pyruvate carboxylase is a potential therapeutic approach for nonalcoholic fatty liver disease, hepatic insulin resistance, and type 2 diabetes. ..
  6. Zhang J, Xia W, Brew K, Ahmad F. Adipose pyruvate carboxylase: amino acid sequence and domain structure deduced from cDNA sequencing. Proc Natl Acad Sci U S A. 1993;90:1766-70 pubmed
  7. Sellers K, Fox M, Bousamra M, Slone S, Higashi R, Miller D, et al. Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation. J Clin Invest. 2015;125:687-98 pubmed publisher
    ..These findings indicate that PC-mediated anaplerosis in early-stage NSCLC is required for tumor survival and proliferation. ..
  8. Martin J, Balmer M, Rajendran S, Maurhofer O, Dufour J, St Pierre M. Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein. Am J Physiol Gastrointest Liver Physiol. 2016;310:G497-509 pubmed publisher
    ..The absence of Hint2 deregulates the posttranslational modification of several mitochondrial proteins, which impedes the adaptation to episodes of nutritional stress. ..
  9. Rochelle J, Watson M, Oakey R, Seldin M. A linkage map of mouse chromosome 19: definition of comparative mapping relationships with human chromosomes 10 and 11 including the MEN1 locus. Genomics. 1992;14:26-31 pubmed
  10. Harrison S, Sozen B, Christodoulou N, Kyprianou C, Zernicka Goetz M. Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro. Science. 2017;356: pubmed publisher
    ..Our study demonstrates the ability of distinct stem cell types to self-assemble in vitro to generate embryos whose morphogenesis, architecture, and constituent cell types resemble those of natural embryos. ..