Ocln

Summary

Gene Symbol: Ocln
Description: occludin
Alias: AI503564, Ocl, occludin
Species: mouse
Products:     Ocln

Top Publications

  1. Murata M, Kojima T, Yamamoto T, Go M, Takano K, Osanai M, et al. Down-regulation of survival signaling through MAPK and Akt in occludin-deficient mouse hepatocytes in vitro. Exp Cell Res. 2005;310:140-51 pubmed
    ..b>Occludin was the first integral membrane protein to be discovered, but it is not indispensable for the formation of TJ ..
  2. Morita K, Itoh M, Saitou M, Ando Akatsuka Y, Furuse M, Yoneda K, et al. Subcellular distribution of tight junction-associated proteins (occludin, ZO-1, ZO-2) in rodent skin. J Invest Dermatol. 1998;110:862-6 pubmed
    b>Occludin is an integral membrane protein that is concentrated at tight junctions (zonulae occludentes) in simple epithelial cells...
  3. Furuse M, Sasaki H, Fujimoto K, Tsukita S. A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts. J Cell Biol. 1998;143:391-401 pubmed
    Three integral membrane proteins, clau- din-1, -2, and occludin, are known to be components of tight junction (TJ) strands. To examine their ability to form TJ strands, their cDNAs were introduced into mouse L fibroblasts lacking TJs...
  4. Morimoto S, Nishimura N, Terai T, Manabe S, Yamamoto Y, Shinahara W, et al. Rab13 mediates the continuous endocytic recycling of occludin to the cell surface. J Biol Chem. 2005;280:2220-8 pubmed
    ..b>Occludin and claudins are integral membrane proteins comprising TJ strands and are thought to be responsible for ..
  5. Stamatovic S, Keep R, Wang M, Jankovic I, Andjelkovic A. Caveolae-mediated internalization of occludin and claudin-5 during CCL2-induced tight junction remodeling in brain endothelial cells. J Biol Chem. 2009;284:19053-66 pubmed publisher
    ..It investigates the potential role of selective internalization of TJ transmembrane proteins (occludin and claudin-5) in increased permeability of the brain endothelial barrier in vitro...
  6. Ikenouchi J, Matsuda M, Furuse M, Tsukita S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J Cell Sci. 2003;116:1959-67 pubmed
    ..Claudins and occludin are integral membrane proteins localized at tight junctions, which are responsible for establishing and ..
  7. Marchiando A, Shen L, Graham W, Weber C, Schwarz B, Austin J, et al. Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo. J Cell Biol. 2010;189:111-26 pubmed publisher
    ..We have used in vivo imaging and transgenic mice expressing fluorescent-tagged occludin and ZO-1 fusion proteins to link occludin endocytosis to TNF-induced tight junction regulation...
  8. Katsuno T, Umeda K, Matsui T, Hata M, Tamura A, Itoh M, et al. Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells. Mol Biol Cell. 2008;19:2465-75 pubmed publisher
    ..In conclusion, ZO-1 may be functionally important for cell remodeling and tissue organization in both the embryonic and extraembryonic regions, thus playing an essential role in embryonic development...
  9. O Driscoll M, Daly S, Urquhart J, Black G, Pilz D, Brockmann K, et al. Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyria. Am J Hum Genet. 2010;87:354-64 pubmed publisher
    ..The OCLN gene encodes occludin, an integral component of tight junctions...
  10. Kirschner N, Haftek M, Niessen C, Behne M, Furuse M, Moll I, et al. CD44 regulates tight-junction assembly and barrier function. J Invest Dermatol. 2011;131:932-43 pubmed publisher
    ..Taken together, the results reveal an important function for CD44 in the assembly and function of TJs, suggesting their involvement in the skin barrier phenotype of CD44 KO mice. ..

Detail Information

Publications92

  1. Murata M, Kojima T, Yamamoto T, Go M, Takano K, Osanai M, et al. Down-regulation of survival signaling through MAPK and Akt in occludin-deficient mouse hepatocytes in vitro. Exp Cell Res. 2005;310:140-51 pubmed
    ..b>Occludin was the first integral membrane protein to be discovered, but it is not indispensable for the formation of TJ ..
  2. Morita K, Itoh M, Saitou M, Ando Akatsuka Y, Furuse M, Yoneda K, et al. Subcellular distribution of tight junction-associated proteins (occludin, ZO-1, ZO-2) in rodent skin. J Invest Dermatol. 1998;110:862-6 pubmed
    b>Occludin is an integral membrane protein that is concentrated at tight junctions (zonulae occludentes) in simple epithelial cells...
  3. Furuse M, Sasaki H, Fujimoto K, Tsukita S. A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts. J Cell Biol. 1998;143:391-401 pubmed
    Three integral membrane proteins, clau- din-1, -2, and occludin, are known to be components of tight junction (TJ) strands. To examine their ability to form TJ strands, their cDNAs were introduced into mouse L fibroblasts lacking TJs...
  4. Morimoto S, Nishimura N, Terai T, Manabe S, Yamamoto Y, Shinahara W, et al. Rab13 mediates the continuous endocytic recycling of occludin to the cell surface. J Biol Chem. 2005;280:2220-8 pubmed
    ..b>Occludin and claudins are integral membrane proteins comprising TJ strands and are thought to be responsible for ..
  5. Stamatovic S, Keep R, Wang M, Jankovic I, Andjelkovic A. Caveolae-mediated internalization of occludin and claudin-5 during CCL2-induced tight junction remodeling in brain endothelial cells. J Biol Chem. 2009;284:19053-66 pubmed publisher
    ..It investigates the potential role of selective internalization of TJ transmembrane proteins (occludin and claudin-5) in increased permeability of the brain endothelial barrier in vitro...
  6. Ikenouchi J, Matsuda M, Furuse M, Tsukita S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J Cell Sci. 2003;116:1959-67 pubmed
    ..Claudins and occludin are integral membrane proteins localized at tight junctions, which are responsible for establishing and ..
  7. Marchiando A, Shen L, Graham W, Weber C, Schwarz B, Austin J, et al. Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo. J Cell Biol. 2010;189:111-26 pubmed publisher
    ..We have used in vivo imaging and transgenic mice expressing fluorescent-tagged occludin and ZO-1 fusion proteins to link occludin endocytosis to TNF-induced tight junction regulation...
  8. Katsuno T, Umeda K, Matsui T, Hata M, Tamura A, Itoh M, et al. Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells. Mol Biol Cell. 2008;19:2465-75 pubmed publisher
    ..In conclusion, ZO-1 may be functionally important for cell remodeling and tissue organization in both the embryonic and extraembryonic regions, thus playing an essential role in embryonic development...
  9. O Driscoll M, Daly S, Urquhart J, Black G, Pilz D, Brockmann K, et al. Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyria. Am J Hum Genet. 2010;87:354-64 pubmed publisher
    ..The OCLN gene encodes occludin, an integral component of tight junctions...
  10. Kirschner N, Haftek M, Niessen C, Behne M, Furuse M, Moll I, et al. CD44 regulates tight-junction assembly and barrier function. J Invest Dermatol. 2011;131:932-43 pubmed publisher
    ..Taken together, the results reveal an important function for CD44 in the assembly and function of TJs, suggesting their involvement in the skin barrier phenotype of CD44 KO mice. ..
  11. Saitou M, Fujimoto K, Doi Y, Itoh M, Fujimoto T, Furuse M, et al. Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions. J Cell Biol. 1998;141:397-408 pubmed
    b>Occludin is the only known integral membrane protein of tight junctions (TJs), and is now believed to be directly involved in the barrier and fence functions of TJs...
  12. Ando Akatsuka Y, Saitou M, Hirase T, Kishi M, Sakakibara A, Itoh M, et al. Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J Cell Biol. 1996;133:43-7 pubmed
    b>Occludin has been identified from chick liver as a novel integral membrane protein localizing at tight junctions (Furuse, M., T. Hirase, M. Itoh, A. Nagafuchi, S. Yonemura, Sa. Tsukita, and Sh. Tsukita. 1993. J. Cell Biol. 123:1777-1788)...
  13. Ikenouchi J, Furuse M, Furuse K, Sasaki H, Tsukita S, Tsukita S. Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells. J Cell Biol. 2005;171:939-45 pubmed
    ..These findings indicate the critical function of tricellulin for formation of the epithelial barrier. ..
  14. Saitou M, Furuse M, Sasaki H, Schulzke J, Fromm M, Takano H, et al. Complex phenotype of mice lacking occludin, a component of tight junction strands. Mol Biol Cell. 2000;11:4131-42 pubmed
    b>Occludin is an integral membrane protein with four transmembrane domains that is exclusively localized at tight junction (TJ) strands. Here, we describe the generation and analysis of mice carrying a null mutation in the occludin gene...
  15. Schulzke J, Gitter A, Mankertz J, Spiegel S, Seidler U, Amasheh S, et al. Epithelial transport and barrier function in occludin-deficient mice. Biochim Biophys Acta. 2005;1669:34-42 pubmed
    This study aimed at functional characterization of the tight junction protein occludin using the occludin-deficient mouse model. Epithelial transport and barrier functions were characterized in Ussing chambers...
  16. Traweger A, Fang D, Liu Y, Stelzhammer W, Krizbai I, Fresser F, et al. The tight junction-specific protein occludin is a functional target of the E3 ubiquitin-protein ligase itch. J Biol Chem. 2002;277:10201-8 pubmed
    ..Recent evidence confirms the notion that occludin, a four-pass integral plasma-membrane protein, is a functional component of the paracellular barrier...
  17. Kanatsu Shinohara M, Ikawa M, Takehashi M, Ogonuki N, Miki H, Inoue K, et al. Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells. Proc Natl Acad Sci U S A. 2006;103:8018-23 pubmed
    ..Using this strategy, the efficiency of homologous recombination for the occludin gene locus was 1.7% using a nonisogenic DNA construct...
  18. Osanai M, Murata M, Nishikiori N, Chiba H, Kojima T, Sawada N. Occludin-mediated premature senescence is a fail-safe mechanism against tumorigenesis in breast carcinoma cells. Cancer Sci. 2007;98:1027-34 pubmed
    We have previously demonstrated that epigenetic silencing of occludin, a tight junction-associated membrane protein, results in the acquisition of apoptotic resistance to various apoptogenic stimuli, causally contributing to the enhanced ..
  19. Takekuni K, Ikeda W, Fujito T, Morimoto K, Takeuchi M, Monden M, et al. Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing mouse. J Biol Chem. 2003;278:5497-500 pubmed
    ..These results indicate that nectin-1 and -3 are involved in the localization of PAR-3 at AJs in the neuroepithelial cells of the embryonic telencephalon. ..
  20. Citi S, Cordenonsi M. Tight junction proteins. Biochim Biophys Acta. 1998;1448:1-11 pubmed
  21. Schmidt A, Utepbergenov D, Krause G, Blasig I. Use of surface plasmon resonance for real-time analysis of the interaction of ZO-1 and occludin. Biochem Biophys Res Commun. 2001;288:1194-9 pubmed
    ..spectroscopy was applied to study in real time, the interaction between the tight junction proteins ZO-1 and occludin. To imitate the morphology of tight junctions, a cytosolic tail of mouse occludin was immobilised at the sensor ..
  22. Youssef G, Gerner L, Naeem A, Ralph O, Ono M, O Neill C, et al. Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition. Dev Biol. 2013;380:274-85 pubmed publisher
    ..This was accompanied by a change of Claudin-1 localisation to the cell surface and interaction between Occludin and Claudin-1 which are thought to be required for tight junction formation...
  23. Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, et al. EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins. Dev Biol. 2012;371:136-45 pubmed publisher
    ..Finally, the barrier function of the intestinal epithelium was impaired in the mutant animals. These results suggest that EpCAM contributes to formation of intestinal barrier by recruiting claudins to cell-cell junctions. ..
  24. Saint Geniez M, Kurihara T, Sekiyama E, Maldonado A, D Amore P. An essential role for RPE-derived soluble VEGF in the maintenance of the choriocapillaris. Proc Natl Acad Sci U S A. 2009;106:18751-6 pubmed publisher
    ..These changes are reminiscent of geographic atrophy (GA) and point to a role for RPE-derived VEGF in the maintenance of the choriocapillaris. ..
  25. Nakatsu D, Kano F, Taguchi Y, Sugawara T, Nishizono T, Nishikawa K, et al. JNK1/2-dependent phosphorylation of angulin-1/LSR is required for the exclusive localization of angulin-1/LSR and tricellulin at tricellular contacts in EpH4 epithelial sheet. Genes Cells. 2014;19:565-81 pubmed publisher
  26. Yu T, Lu X, Li J, Shan T, Huang C, Ouyang H, et al. Overexpression of miR-429 impairs intestinal barrier function in diabetic mice by down-regulating occludin expression. Cell Tissue Res. 2016;366:341-352 pubmed publisher
    ..We previously reported the presence of an impaired tight junction and decreased expression of occludin (Ocln) and zonula occludens-1 (ZO-1) in the intestinal epithelial cells (IECs) of type 1 DM mice, but the exact ..
  27. Chiplunkar A, Curtis B, Eades G, Kane M, Fox S, Haar J, et al. The Krüppel-like factor 2 and Krüppel-like factor 4 genes interact to maintain endothelial integrity in mouse embryonic vasculogenesis. BMC Dev Biol. 2013;13:40 pubmed publisher
    ..In adult mice, KLF2 regulates expression of the tight junction protein occludin, which may allow KLF2 to maintain vascular integrity...
  28. Kusaka M, Katoh Fukui Y, Ogawa H, Miyabayashi K, Baba T, Shima Y, et al. Abnormal epithelial cell polarity and ectopic epidermal growth factor receptor (EGFR) expression induced in Emx2 KO embryonic gonads. Endocrinology. 2010;151:5893-904 pubmed publisher
    ..Our results strongly suggest that Emx2 is required for regulation of tight junction assembly and allowing migration of the gonadal epithelia to the mesenchyme, which are possibly mediated by suppression of Egfr expression...
  29. Park K, Lee M, Jeon Y, Jeon R, Baek S, Lee H, et al. Skin-Specific Deletion of Mis18? Impedes Proliferation and Stratification of Epidermal Keratinocytes. J Invest Dermatol. 2017;137:414-421 pubmed publisher
    ..Overall, we propose that Mis18? is important for epidermal cell proliferation and stratification, because it is required for the deposition of CENP-A at the centromeric nucleosomes. ..
  30. Werth M, Walentin K, Aue A, Schönheit J, Wuebken A, Pode Shakked N, et al. The transcription factor grainyhead-like 2 regulates the molecular composition of the epithelial apical junctional complex. Development. 2010;137:3835-45 pubmed publisher
    ..Together, our data provide evidence that Grhl2 acts as a target gene-associated transcriptional activator of apical junctional complex components and, thereby, crucially participates in epithelial differentiation. ..
  31. Cyr D, Hermo L, Egenberger N, Mertineit C, Trasler J, Laird D. Cellular immunolocalization of occludin during embryonic and postnatal development of the mouse testis and epididymis. Endocrinology. 1999;140:3815-25 pubmed
    ..In the present study, occludin, a constituent integral membrane protein of tight junctions, was localized by immunofluorescent confocal ..
  32. Chihara M, Otsuka S, Ichii O, Hashimoto Y, Kon Y. Molecular dynamics of the blood-testis barrier components during murine spermatogenesis. Mol Reprod Dev. 2010;77:630-9 pubmed publisher
    ..Structural analysis revealed that the principal components of the BTB, for example, claudin-3, claudin-11, occludin, and zonula occludens-1 (ZO-1), were localized at the basal and luminal sides of the preleptotene/leptotene ..
  33. Alvarez J, Dodelet Devillers A, Kebir H, Ifergan I, Fabre P, Terouz S, et al. The Hedgehog pathway promotes blood-brain barrier integrity and CNS immune quiescence. Science. 2011;334:1727-31 pubmed publisher
    ..Overall, the Hh pathway provides a barrier-promoting effect and an endogenous anti-inflammatory balance to CNS-directed immune attacks, as occurs in multiple sclerosis. ..
  34. Campbell M, Collery R, McEvoy A, Gardiner T, Stitt A, Brankin B. Involvement of MAPKs in endostatin-mediated regulation of blood-retinal barrier function. Curr Eye Res. 2006;31:1033-45 pubmed
    ..Moreover, it was hypothesized that endostatin-induced occludin upregulation regulated VEGF165-mediated increases in endothelial cell permeability and involved activation of the ..
  35. Xie L, Xue X, Taylor M, Ramakrishnan S, Nagaoka K, Hao C, et al. Hypoxia-inducible factor/MAZ-dependent induction of caveolin-1 regulates colon permeability through suppression of occludin, leading to hypoxia-induced inflammation. Mol Cell Biol. 2014;34:3013-23 pubmed publisher
    ..Hypoxic induction of CAV1 in the colon was essential for intestinal barrier integrity by regulating occludin expression...
  36. Itoh M, Morita K, Tsukita S. Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin. J Biol Chem. 1999;274:5981-6 pubmed
    ..Consistently, occludin as well as alpha catenin directly bound to N-ZO-2 as well as the NH2-terminal dlg-like portion of ZO-1 (N-ZO-1) ..
  37. Zhong Z, Deane R, Ali Z, Parisi M, Shapovalov Y, O Banion M, et al. ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration. Nat Neurosci. 2008;11:420-2 pubmed publisher
    ..disrupted the blood-spinal cord barrier in mice by reducing the levels of the tight junction proteins ZO-1, occludin and claudin-5 between endothelial cells...
  38. Raleigh D, Marchiando A, Zhang Y, Shen L, Sasaki H, Wang Y, et al. Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions. Mol Biol Cell. 2010;21:1200-13 pubmed publisher
    In vitro studies have demonstrated that occludin and tricellulin are important for tight junction barrier function, but in vivo data suggest that loss of these proteins can be overcome...
  39. Chen Y, Zhang H, Fong G, Xi Q, Wu G, Bai C, et al. PHD3 Stabilizes the Tight Junction Protein Occludin and Protects Intestinal Epithelial Barrier Function. J Biol Chem. 2015;290:20580-9 pubmed publisher
    ..Deletion of PHD3 decreases the level of tight junction protein occludin, leading to a failure of intestinal epithelial barrier function...
  40. Ohsugi M, Larue L, Schwarz H, Kemler R. Cell-junctional and cytoskeletal organization in mouse blastocysts lacking E-cadherin. Dev Biol. 1997;185:261-71 pubmed
    ..against alpha-, beta-, or gamma-(plakoglobin)-catenin and junctional and cytoskeletal proteins including ZO-1 and occludin (tight junctions), desmoglein1 (desmosomes), connexin43 (gap junctions), and EndoA (cytokeratin intermediate ..
  41. Daneman R, Agalliu D, Zhou L, Kuhnert F, Kuo C, Barres B. Wnt/beta-catenin signaling is required for CNS, but not non-CNS, angiogenesis. Proc Natl Acad Sci U S A. 2009;106:641-6 pubmed publisher
    ..Taken together these experiments reveal an essential role for Wnt/beta-catenin signaling in driving CNS-specific angiogenesis and provide molecular evidence that angiogenesis and BBB formation are in part linked. ..
  42. Sheth B, Fontaine J, Ponza E, McCallum A, Page A, Citi S, et al. Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction. Mech Dev. 2000;97:93-104 pubmed
    ..However, following assembly of the TJ transmembrane protein, occludin, from the early 32-cell stage when blastocoel formation begins, ZO-1alpha- and other TJ proteins (ZO-1alpha+ ..
  43. Nunes F, Lopez L, Lin H, Davies C, Azevedo R, Gow A, et al. Distinct subdomain organization and molecular composition of a tight junction with adherens junction features. J Cell Sci. 2006;119:4819-27 pubmed
  44. Liu L, Jiang Y, Steinle J. Toll-Like Receptor 4 Reduces Occludin and Zonula Occludens 1 to Increase Retinal Permeability Both in vitro and in vivo. J Vasc Res. 2017;54:367-375 pubmed publisher
    ..siRNA treatments for cell culture studies of permeability and protein analyses of tumor necrosis factor α, occludin, and zonula occludens 1 (ZO-1)...
  45. Barron M, Brookes S, Draper C, Garrod D, Kirkham J, Shore R, et al. The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice. Hum Mol Genet. 2008;17:3509-20 pubmed publisher
    ..Importantly, our results show that integrity of the SI-ameloblast interface is essential for normal enamel mineralization. ..
  46. Iizuka Kogo A, Ishidao T, Akiyama T, Senda T. Abnormal development of urogenital organs in Dlgh1-deficient mice. Development. 2007;134:1799-807 pubmed
    ..Our findings might contribute to a better understanding of such abnormalities. ..
  47. Foote H, Sumigray K, Lechler T. FRAP analysis reveals stabilization of adhesion structures in the epidermis compared to cultured keratinocytes. PLoS ONE. 2013;8:e71491 pubmed publisher
    ..Our data demonstrate that there are additional mechanisms for stabilizing junctions in tissues that are not modeled by cell culture. ..
  48. Adachi M, Inoko A, Hata M, Furuse K, Umeda K, Itoh M, et al. Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein. Mol Cell Biol. 2006;26:9003-15 pubmed
    ..These findings indicate that ZO-3 is dispensable in vivo in terms of individual viability, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment. ..
  49. Tanaka H, Yamamoto Y, Kashihara H, Yamazaki Y, Tani K, Fujiyoshi Y, et al. Claudin-21 Has a Paracellular Channel Role at Tight Junctions. Mol Cell Biol. 2016;36:954-64 pubmed publisher
    ..Claudin-21 also allows the paracellular passage of larger solutes. Our findings suggest that claudin-21-based TJs allow the passage of small and larger solutes by both paracellular channel-based and some additional mechanisms. ..
  50. Liu X, Dreffs A, Díaz Coránguez M, Runkle E, Gardner T, Chiodo V, et al. Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth Factor-Induced Retinal Neovascularization. Am J Pathol. 2016;186:2486-99 pubmed publisher
    b>Occludin is a transmembrane tight junction protein that contributes to diverse cellular functions, including control of barrier properties, cell migration, and proliferation...
  51. Seidl S, Braun U, Roos N, Li S, Lüdtke T, Kispert A, et al. Phenotypical analysis of atypical PKCs in vivo function display a compensatory system at mouse embryonic day 7.5. PLoS ONE. 2013;8:e62756 pubmed publisher
    ..We present a compensatory function of PKC? at E7.5, rescuing the phenotype. Furthermore, this study indicates at least one specific, yet unknown, PKC? function that cannot be compensated by the overexpression of PKC? at E9.5. ..
  52. Thomas T, Voss A, Petrou P, Gruss P. The murine gene, Traube, is essential for the growth of preimplantation embryos. Dev Biol. 2000;227:324-42 pubmed
    ..This paucity of ribosomes together with the localization of TRB to the nucleoli, the site of ribosome synthesis, suggests that TRB is involved in the synthesis of ribosomes. ..
  53. Hellstrom M, Gerhardt H, Kalén M, Li X, Eriksson U, Wolburg H, et al. Lack of pericytes leads to endothelial hyperplasia and abnormal vascular morphogenesis. J Cell Biol. 2001;153:543-53 pubmed
  54. Andreeva A, Krause E, Müller E, Blasig I, Utepbergenov D. Protein kinase C regulates the phosphorylation and cellular localization of occludin. J Biol Chem. 2001;276:38480-6 pubmed
    b>Occludin is an integral membrane phosphoprotein specifically associated with tight junctions, contributing to the structure and function of this intercellular seal...
  55. Van Itallie C, Mitic L, Anderson J. Claudin-2 forms homodimers and is a component of a high molecular weight protein complex. J Biol Chem. 2011;286:3442-50 pubmed publisher
    ..identified a relatively discrete high molecular weight complex containing at least claudin-1, claudin-2, and occludin; the difference in the protein complex sizes between cultured cells and tissues may reflect differences in tight ..
  56. Volpert O, Pili R, Sikder H, Nelius T, Zaichuk T, Morris C, et al. Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1. Cancer Cell. 2002;2:473-83 pubmed
    ..This decrease was completely reversed by a TSP-1 neutralizing antibody. We conclude that TSP-1 is a major target for Id1 effects on angiogenesis. ..
  57. Lee J, Tato C, Joyce Shaikh B, Gulen M, Gulan F, Cayatte C, et al. Interleukin-23-Independent IL-17 Production Regulates Intestinal Epithelial Permeability. Immunity. 2015;43:727-38 pubmed publisher
    ..Here we have provided evidence for IL-17A-dependent regulation of the tight junction protein occludin during epithelial injury that limits excessive permeability and maintains barrier integrity...
  58. Etournay R, Zwaenepoel I, Perfettini I, Legrain P, Petit C, El Amraoui A. Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions. J Cell Sci. 2007;120:2838-50 pubmed
  59. Yamamoto M, Ramirez S, Sato S, Kiyota T, Cerny R, Kaibuchi K, et al. Phosphorylation of claudin-5 and occludin by rho kinase in brain endothelial cells. Am J Pathol. 2008;172:521-33 pubmed publisher
    ..Posttranslational modifications of essential endothelial TJ proteins, occludin and claudin-5, contribute and possibly disrupt BBB integrity...
  60. Shiojiri N, Sano M, Inujima S, Nitou M, Kanazawa M, Mori M. Quantitative analysis of cell allocation during liver development, using the spf(ash)-heterozygous female mouse. Am J Pathol. 2000;156:65-75 pubmed
    ..Immunohistochemical analysis of intercellular junction proteins (E-cadherin, connexins 26 and 32, occludin, and ZO-1) also revealed that their expression and distribution changed in hepatocytes during development, which ..
  61. Ray S, Foote H, Lechler T. beta-Catenin protects the epidermis from mechanical stresses. J Cell Biol. 2013;202:45-52 pubmed publisher
    ..These data demonstrate that a complete understanding of the functions of cell adhesion proteins must take into account their roles in response to mechanical stresses. ..
  62. Growney J, Scharf J, Kunkel L, Dietrich W. Evolutionary divergence of the mouse and human Lgn1/SMA repeat structures. Genomics. 2000;64:62-81 pubmed
    ..Several, but not all, of these Naip loci are contained within the Lgn1 critical interval. We present a model for the origins of the mouse and human repetitive arrays from a common ancestral haplotype. ..
  63. Nishimura N, Sasaki T. Cell-surface biotinylation to study endocytosis and recycling of occludin. Methods Mol Biol. 2008;440:89-96 pubmed publisher
    ..b>Occludin and claudins are distinct transmembrane proteins localized to the TJs...
  64. Kranz J, Petzinger E, Geyer J. Brain penetration of the OAB drug trospium chloride is not increased in aged mice. World J Urol. 2013;31:219-24 pubmed publisher
    ..Furthermore, mRNA expression levels of relevant markers of blood-brain barrier integrity (occludin, claudin-5, and the drug efflux carrier P-glycoprotein) were analysed in brain samples from adult and aged mice...
  65. Kitajiri S, Katsuno T, Sasaki H, Ito J, Furuse M, Tsukita S. Deafness in occludin-deficient mice with dislocation of tricellulin and progressive apoptosis of the hair cells. Biol Open. 2014;3:759-66 pubmed publisher
    b>Occludin is the first identified protein in the tight junction (TJ), but its function has remained for the most part obscure...
  66. Yun J, Park S, Kim K, Bae J, Lee E, Paek S, et al. Endothelial STAT3 Activation Increases Vascular Leakage Through Downregulating Tight Junction Proteins: Implications for Diabetic Retinopathy. J Cell Physiol. 2017;232:1123-1134 pubmed publisher
    ..and vascular leakage in retinas of mice through the reduced expression of the tight junction proteins ZO-1 and occludin. In a co-culture model with microglia and endothelial cells under a high glucose condition, the microglia-derived ..
  67. Mellas R, Leigh N, Nelson J, McCall A, Baker O. Zonula occludens-1, occludin and E-cadherin expression and organization in salivary glands with Sjögren's syndrome. J Histochem Cytochem. 2015;63:45-56 pubmed publisher
    ..Protein expression studies revealed that the TJ proteins, zonula occludens-1 (ZO-1), occludin, claudin-12, as well as E-cadherin, do not significantly change in NOD/ShiLtJ mice...
  68. Ohta H, Adachi H, Inaba M. Developmental changes in the expression of tight junction protein claudins in murine metanephroi and embryonic kidneys. J Vet Med Sci. 2006;68:149-55 pubmed
    ..chain reaction for the claudins examined, including claudins 1-4, 8, 10, 11, and 16, and the TJ proteins occludin and ZO-1...
  69. Hata M, Kawamoto T, Kawai M, Yamamoto T. Differential expression patterns of the tight junction-associated proteins occludin and claudins in secretory and mature ameloblasts in mouse incisor. Med Mol Morphol. 2010;43:102-6 pubmed publisher
    ..primarily as a barrier against paracellular transport between epithelial cells and are composed mainly of occludin (OLD) and claudins (CLDs). The CLD family consists of 24 members that show tissue- or cell-specific expression...
  70. Kim G, Georg I, Scherthan H, Merkenschlager M, Guillou F, Scherer G, et al. Dicer is required for Sertoli cell function and survival. Int J Dev Biol. 2010;54:867-75 pubmed publisher
    ..Taken together, the results of this study show that Dicer is required for Sertoli cell function and survival and for spermatogenesis in mice. ..
  71. Lozovatsky L, Abayasekara N, Piawah S, Walther Z. CASK deletion in intestinal epithelia causes mislocalization of LIN7C and the DLG1/Scrib polarity complex without affecting cell polarity. Mol Biol Cell. 2009;20:4489-99 pubmed publisher
    ..Thus, CASK expression and the appropriate localization of DLG1 are not essential for either epithelial polarity or intestinal homeostasis in vivo. ..
  72. Nakai K, Yoneda K, Hosokawa Y, Moriue T, Presland R, Fallon P, et al. Reduced expression of epidermal growth factor receptor, E-cadherin, and occludin in the skin of flaky tail mice is due to filaggrin and loricrin deficiencies. Am J Pathol. 2012;181:969-77 pubmed publisher
    ..Herein, we report the decreased expression of epidermal growth factor receptor (EGFR), E-cadherin, occludin, and SIRT1 in the skin of Flg(ft) mice, compared with those in C57BL/6J mice...
  73. Al Sadi R, Khatib K, Guo S, Ye D, Youssef M, Ma T. Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier. Am J Physiol Gastrointest Liver Physiol. 2011;300:G1054-64 pubmed publisher
    ..The expression of occludin is markedly decreased in intestinal permeability disorders, including in Crohn's disease, ulcerative colitis, and ..
  74. Beeman N, Baumgartner H, Webb P, Schaack J, Neville M. Disruption of occludin function in polarized epithelial cells activates the extrinsic pathway of apoptosis leading to cell extrusion without loss of transepithelial resistance. BMC Cell Biol. 2009;10:85 pubmed publisher
    b>Occludin is a tetraspanin protein normally localized to tight junctions. The protein interacts with a variety of pathogens including viruses and bacteria, an interaction that sometimes leads to its extrajunctional localization...
  75. Kobayashi K, Yasui M. Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice. Cell Tissue Res. 2010;342:307-16 pubmed publisher
  76. Kyoko O, Kono H, Ishimaru K, Miyake K, Kubota T, Ogawa H, et al. Expressions of tight junction proteins Occludin and Claudin-1 are under the circadian control in the mouse large intestine: implications in intestinal permeability and susceptibility to colitis. PLoS ONE. 2014;9:e98016 pubmed publisher
    ..The mRNA and protein expression levels of Occludin and Claudin-1 exhibited daily variations in the colonic epithelium in wild-type mice, whereas they were ..
  77. Patel R, Myers L, Kurundkar A, Maheshwari A, Nusrat A, Lin P. Probiotic bacteria induce maturation of intestinal claudin 3 expression and barrier function. Am J Pathol. 2012;180:626-35 pubmed publisher
    ..Probiotics may prevent intestinal inflammatory diseases by regulating intestinal tight junction protein expression and barrier function. The use of heat-killed probiotics may provide therapeutic benefit while minimizing adverse effects. ..
  78. Szabo S, Wögenstein K, Osterreicher C, Guldiken N, Chen Y, Doler C, et al. Epiplakin attenuates experimental mouse liver injury by chaperoning keratin reorganization. J Hepatol. 2015;62:1357-66 pubmed publisher
    ..Our data indicate that epiplakin plays a protective role during experimental liver injuries by chaperoning disease-induced keratin reorganization. ..
  79. Cording J, Günther R, Vigolo E, Tscheik C, Winkler L, Schlattner I, et al. Redox Regulation of Cell Contacts by Tricellulin and Occludin: Redox-Sensitive Cysteine Sites in Tricellulin Regulate Both Tri- and Bicellular Junctions in Tissue Barriers as Shown in Hypoxia and Ischemia. Antioxid Redox Signal. 2015;23:1035-49 pubmed publisher
    ..TJ-associated marvel proteins (TAMPs; tricellulin, occludin, marvelD3) are thought to be relevant to regulation...
  80. Phoenix T, Patmore D, Boop S, Boulos N, Jacus M, Patel Y, et al. Medulloblastoma Genotype Dictates Blood Brain Barrier Phenotype. Cancer Cell. 2016;29:508-522 pubmed publisher
    ..Thus, medulloblastoma genotype dictates tumor vessel phenotype, explaining in part the disparate prognoses among medulloblastoma subtypes and suggesting an approach to enhance the chemoresponsiveness of other brain tumors. ..
  81. Raschperger E, Thyberg J, Pettersson S, Philipson L, Fuxe J, Pettersson R. The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis. Exp Cell Res. 2006;312:1566-80 pubmed
    ..the body cavities in adult mice, where it specifically co-localizes with the tight junction components ZO-1 and occludin. Notably, CAR could not be detected in endothelial cells of the vasculature, including brain capillaries...
  82. Zega K, Jovanovic V, Vitic Z, Niedzielska M, Knaapi L, Jukic M, et al. Dusp16 Deficiency Causes Congenital Obstructive Hydrocephalus and Brain Overgrowth by Expansion of the Neural Progenitor Pool. Front Mol Neurosci. 2017;10:372 pubmed publisher
    ..Moreover our results suggest that a lack of functional Dusp16 could play a central role in the molecular mechanisms linking brain overgrowth and hydrocephalus. ..
  83. Yao L, Baluk P, Srinivasan R, Oliver G, McDonald D. Plasticity of button-like junctions in the endothelium of airway lymphatics in development and inflammation. Am J Pathol. 2012;180:2561-75 pubmed publisher
    ..These findings demonstrate the plasticity of intercellular junctions in lymphatics during development and inflammation and show that button formation can be promoted by glucocorticoid receptor signaling in lymphatic endothelial cells...
  84. Peng H, Luo P, Li Y, Wang C, Liu X, Ye Z, et al. Simvastatin alleviates hyperpermeability of glomerular endothelial cells in early-stage diabetic nephropathy by inhibition of RhoA/ROCK1. PLoS ONE. 2013;8:e80009 pubmed publisher
    ..We assessed the expression of occludin and ZO-1, two major components of the tight junction complex, in cultured rat GEnCs treated with high glucose and ..
  85. Xu H, Dawson R, Crane I, Liversidge J. Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier. Invest Ophthalmol Vis Sci. 2005;46:2487-94 pubmed
    ..The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permeability are controversial...
  86. Tunggal J, Helfrich I, Schmitz A, Schwarz H, Gunzel D, Fromm M, et al. E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions. EMBO J. 2005;24:1146-56 pubmed
    ..Surprisingly, our results indicate that E-cadherin is specifically required for tight junction, but not desmosome, formation and this appears to involve signalling rather than cell contact formation. ..
  87. Sheth B, Moran B, Anderson J, Fleming T. Post-translational control of occludin membrane assembly in mouse trophectoderm: a mechanism to regulate timing of tight junction biogenesis and blastocyst formation. Development. 2000;127:831-40 pubmed
    ..mechanisms regulating the timing of blastocyst formation, we have examined the dynamics of expression of occludin, an integral membrane protein of the TJ...
  88. Chen F, Ohashi N, Li W, Eckman C, Nguyen J. Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure. Hepatology. 2009;50:1914-23 pubmed publisher
    ..Endogenous overexpression of MMP-9 in brain ECs resulted in significant degradation of the TJ proteins occludin and claudin-5...
  89. Yu Z, Lin K, Bhandari A, Spencer J, Xu X, Wang N, et al. The Grainyhead-like epithelial transactivator Get-1/Grhl3 regulates epidermal terminal differentiation and interacts functionally with LMO4. Dev Biol. 2006;299:122-36 pubmed
    ..These findings indicate that the Get-1 and LMO4 genes interact functionally to regulate epidermal terminal differentiation. ..
  90. Fujita H, Chiba H, Yokozaki H, Sakai N, Sugimoto K, Wada T, et al. Differential expression and subcellular localization of claudin-7, -8, -12, -13, and -15 along the mouse intestine. J Histochem Cytochem. 2006;54:933-44 pubmed
    ..This manuscript contains online supplemental material available at http://www.jhc.org. Please visit this article online to view these materials. ..