Gene Symbol: Nsf
Description: N-ethylmaleimide sensitive fusion protein
Alias: AI316878, AU020090, AU067812, SKD2, vesicle-fusing ATPase, N-ethylmaleimide sensitive factor, NEM-sensitive fusion protein, suppressor of K(+) transport growth defect 2, vesicular-fusion protein NSF
Species: mouse
Products:     Nsf

Top Publications

  1. Matsushita K, Morrell C, Cambien B, Yang S, Yamakuchi M, Bao C, et al. Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor. Cell. 2003;115:139-50 pubmed
    ..that mediate vascular inflammation and thrombosis, by regulating the activity of N-ethylmaleimide-sensitive factor (NSF)...
  2. Wang Y, Lauffer B, von Zastrow M, Kobilka B, Xiang Y. N-ethylmaleimide-sensitive factor regulates beta2 adrenoceptor trafficking and signaling in cardiomyocytes. Mol Pharmacol. 2007;72:429-39 pubmed
    ..The human beta2AR carboxyl end binds to the N-ethylmaleimide-sensitive factor (NSF), an ATPase integral to membrane trafficking machinery...
  3. Machka C, Kersten M, Zobawa M, Harder A, Horsch M, Halder T, et al. Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Gene Expr Patterns. 2005;6:94-101 pubmed
    ..The large set of regulated genes identified in this differential expression profiling approach is an important resource for further functional studies. ..
  4. Juriloff D, Harris M, Dewell S, Brown C, Mager D, Gagnier L, et al. Investigations of the genomic region that contains the clf1 mutation, a causal gene in multifactorial cleft lip and palate in mice. Birth Defects Res A Clin Mol Teratol. 2005;73:103-13 pubmed
    ..Gosr2, Wnt9b, Wnt3, Nsf, Arf2, Crhr1, Mapt, Cdc27, Myl4, Itgb3, chr11_20.152, chr11_20.154, chr11_20.155, and chr11_20...
  5. Lee J, Linge H, Ochani K, Lin K, Miller E. N-Ethylmaleimide Sensitive Factor (NSF) Inhibition Prevents Vascular Instability following Gram-Positive Pulmonary Challenge. PLoS ONE. 2016;11:e0157837 pubmed publisher
    ..We hypothesized that N-ethylmaleimide sensitive factor (NSF) regulates exocytosis of inflammatory mediators, such as Angiopoietin-2 (Ang-2), and cytoskeletal stability by ..
  6. Piccoli G, Condliffe S, Bauer M, Giesert F, Boldt K, De Astis S, et al. LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool. J Neurosci. 2011;31:2225-37 pubmed publisher
    ..Our results suggest that LRRK2 modulates synaptic vesicle trafficking and distribution in neurons and in consequence participates in regulating the dynamics between vesicle pools inside the presynaptic bouton...
  7. Liu Y, Cheng K, Gong K, Fu A, Ip N. Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis. J Biol Chem. 2006;281:9852-8 pubmed
    ..N-Ethylmaleimide-sensitive fusion protein (NSF), a crucial factor in vesicular transport and membrane fusion, was identified as one of the Pctaire1 interacting ..
  8. Chen D, Xu W, He P, Medrano E, Whiteheart S. Gaf-1, a gamma -SNAP-binding protein associated with the mitochondria. J Biol Chem. 2001;276:13127-35 pubmed
    The role of alpha/beta-SNAP (Soluble NSF Attachment Protein) in vesicular trafficking is well established; however, the function of the ubiquitously expressed gamma-SNAP remains unclear...
  9. Gerst J. SNAREs and SNARE regulators in membrane fusion and exocytosis. Cell Mol Life Sci. 1999;55:707-34 pubmed
    ..g. 'SNARE-masters') in mediating events leading to membrane fusion. A model to illustrate the dynamic cycling of SNAREs between fusion-incompetent and fusion-competent states, called the SNARE cycle, is presented. ..

More Information


  1. Hoyle J, Phelan J, Bermingham N, Fisher E. Localization of human and mouse N-ethylmaleimide-sensitive factor (NSF) gene: a two-domain member of the AAA family that is involved in membrane fusion. Mamm Genome. 1996;7:850-2 pubmed
  2. Puschel A, O CONNOR V, Betz H. The N-ethylmaleimide-sensitive fusion protein (NSF) is preferentially expressed in the nervous system. FEBS Lett. 1994;347:55-8 pubmed
    b>NSF and SNAPs (soluble NSF attachment proteins), originally identified as cytosolic components of intracellular vesicular transport mechanisms, have recently been implicated in Ca(2+)-triggered neurotransmitter release from synaptic ..
  3. Allen R, Marquart T, Jesse J, Baldán A. Control of very low-density lipoprotein secretion by N-ethylmaleimide-sensitive factor and miR-33. Circ Res. 2014;115:10-22 pubmed publisher
    ..of miR-33, we show that miR-33 limits hepatic secretion of VLDL-TAG by targeting N-ethylmaleimide-sensitive factor (NSF), both in vivo and in primary hepatocytes...
  4. Piccoli G, Onofri F, Cirnaru M, Kaiser C, Jagtap P, Kastenmüller A, et al. Leucine-rich repeat kinase 2 binds to neuronal vesicles through protein interactions mediated by its C-terminal WD40 domain. Mol Cell Biol. 2014;34:2147-61 pubmed publisher
    ..Our data demonstrate a critical role of the WD40 domain within LRRK2 function. ..
  5. Fernandez E, Collins M, Uren R, Kopanitsa M, Komiyama N, Croning M, et al. Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins. Mol Syst Biol. 2009;5:269 pubmed publisher
    ..This targeted TAP tagging strategy is generally applicable to mammalian proteomics and systems biology approaches to disease. ..
  6. Borisovska M, Zhao Y, Tsytsyura Y, Glyvuk N, Takamori S, Matti U, et al. v-SNAREs control exocytosis of vesicles from priming to fusion. EMBO J. 2005;24:2114-26 pubmed
    SNARE proteins (soluble NSF-attachment protein receptors) are thought to be central components of the exocytotic mechanism in neurosecretory cells, but their precise function remained unclear...
  7. Huynh H, Bottini N, Williams S, Cherepanov V, Musumeci L, Saito K, et al. Control of vesicle fusion by a tyrosine phosphatase. Nat Cell Biol. 2004;6:831-9 pubmed
    ..Here, we identify N-ethylmaleimide-sensitive factor (NSF), a key regulator of vesicle fusion, as a substrate for PTP-MEG2...
  8. Juriloff D, Harris M, Dewell S. A digenic cause of cleft lip in A-strain mice and definition of candidate genes for the two loci. Birth Defects Res A Clin Mol Teratol. 2004;70:509-18 pubmed
    ..The clf1 region contains 10 known genes (Arf2, Cdc27, Crhr1, Gosr2, Itgb3, Mapt, Myl4, Nsf, Wnt3, and Wnt9b). The clf2 region contains 17 known genes with human orthologs...
  9. Steinberg J, Huganir R, Linden D. N-ethylmaleimide-sensitive factor is required for the synaptic incorporation and removal of AMPA receptors during cerebellar long-term depression. Proc Natl Acad Sci U S A. 2004;101:18212-6 pubmed
    ..These findings demonstrate that interaction of AMPA receptors with N-ethylmaleimide-sensitive factor plays a critical role in incorporation of AMPA receptors into synapses and for their subsequent removal during cerebellar LTD. ..
  10. Hong H, Chakravarti A, Takahashi J. The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice. Proc Natl Acad Sci U S A. 2004;101:1748-53 pubmed
    ..The hyh mutant provides a valuable in vivo model to study vesicle/membrane trafficking and provides insight into the potential roles of alpha-SNAP in embryogenesis and brain development. ..
  11. Belluzzi E, Gonnelli A, Cirnaru M, Marte A, Plotegher N, Russo I, et al. LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate. Mol Neurodegener. 2016;11:1 pubmed publisher
    ..We have previously observed that LRRK2 interacts with NSF, a hexameric AAA+ ATPase that couples ATP hydrolysis to the disassembling of SNARE proteins allowing them to enter ..
  12. Huang S, Brown B, Craft C. Visual Arrestin 1 acts as a modulator for N-ethylmaleimide-sensitive factor in the photoreceptor synapse. J Neurosci. 2010;30:9381-91 pubmed publisher
    ..In this report, we discovered a novel synaptic interaction between Arr1 and N-ethylmaleimide-sensitive factor (NSF) that is enhanced in a dark environment when mouse photoreceptors are depolarized and the rate of exocytosis is ..
  13. Goto H, Terunuma M, Kanematsu T, Misumi Y, Moss S, Hirata M. Direct interaction of N-ethylmaleimide-sensitive factor with GABA(A) receptor beta subunits. Mol Cell Neurosci. 2005;30:197-206 pubmed
    ..Here, we reveal that GABA(A) receptors interact directly with N-ethylmaleimide-sensitive factor (NSF), a critical regulator of vesicular dependent protein trafficking, as measured by in vitro protein binding and co-..
  14. Matsushita K, Morrell C, Mason R, Yamakuchi M, Khanday F, Irani K, et al. Hydrogen peroxide regulation of endothelial exocytosis by inhibition of N-ethylmaleimide sensitive factor. J Cell Biol. 2005;170:73-9 pubmed
    ..H(2)O(2) regulates exocytosis by inhibiting N-ethylmaleimide sensitive factor (NSF), a protein that regulates membrane fusion events necessary for exocytosis...
  15. Zhu D, Yang Z, Luo Z, Luo S, Xiong W, Mei L. Muscle-specific receptor tyrosine kinase endocytosis in acetylcholine receptor clustering in response to agrin. J Neurosci. 2008;28:1688-96 pubmed publisher
    ..Moreover, we provided evidence for a role of N-ethylmaleimide sensitive factor (NSF) in regulating MuSK endocytosis and subsequent signaling in response to agrin stimulation...
  16. Widberg C, Bryant N, Girotti M, Rea S, James D. Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation. J Biol Chem. 2003;278:35093-101 pubmed
    ..These data suggest that Tomosyn and Munc18c operate at a similar stage of the Syntaxin4 SNARE assembly cycle, which likely primes Syntaxin4 for entry into the ternary SNARE complex. ..
  17. Périer F, Coulter K, Liang H, Radeke C, Gaber R, Vandenberg C. Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast. FEBS Lett. 1994;351:286-90 pubmed
    ..These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane ..
  18. Lin D, Huganir R. PICK1 and phosphorylation of the glutamate receptor 2 (GluR2) AMPA receptor subunit regulates GluR2 recycling after NMDA receptor-induced internalization. J Neurosci. 2007;27:13903-8 pubmed
    ..In addition, mutation of the N-ethylmaleimide-sensitive fusion protein (NSF) binding site in the pH-GluR2 slows receptor recycling...
  19. Chou W, Wang D, McMahon T, Qi Z, Song M, Zhang C, et al. GABAA receptor trafficking is regulated by protein kinase C(epsilon) and the N-ethylmaleimide-sensitive factor. J Neurosci. 2010;30:13955-65 pubmed publisher
    ..Here, we show that protein kinase C? (PKC?) regulates the N-ethylmaleimide-sensitive factor (NSF), an ATPase critical for membrane fusion events, and thereby promotes the trafficking of GABA(A) receptors...
  20. Huang S, Craft C. Potential cellular functions of N-ethylmaleimide sensitive factor in the photoreceptor. Adv Exp Med Biol. 2012;723:791-7 pubmed publisher
    Recent work has established potential new functional roles for NSF in the photoreceptor. First, the interaction of Arr1 and NSF is ATP-dependent, and the N-terminal domain of Arr1 interacts with the N and D1 junctional domains of NSF...
  21. Woodman P. The roles of NSF, SNAPs and SNAREs during membrane fusion. Biochim Biophys Acta. 1997;1357:155-72 pubmed
  22. Gardner S, Takamiya K, Xia J, Suh J, Johnson R, Yu S, et al. Calcium-permeable AMPA receptor plasticity is mediated by subunit-specific interactions with PICK1 and NSF. Neuron. 2005;45:903-15 pubmed
    ..the PDZ domain-containing Protein interacting with C kinase (PICK1) and N-ethylmaleimide sensitive fusion protein (NSF), are specifically required for CARP...