Nos3

Summary

Gene Symbol: Nos3
Description: nitric oxide synthase 3, endothelial cell
Alias: 2310065A03Rik, Nos-3, eNOS, ecNOS, nitric oxide synthase, endothelial, EC-NOS, NOS type III, NOSIII, cNOS, constitutive NOS, endothelial NOS, endothelial nitric oxide synthase
Species: mouse
Products:     Nos3

Top Publications

  1. Wang S, Xu J, Song P, Wu Y, Zhang J, Chul Choi H, et al. Acute inhibition of guanosine triphosphate cyclohydrolase 1 uncouples endothelial nitric oxide synthase and elevates blood pressure. Hypertension. 2008;52:484-90 pubmed publisher
    ..enzyme in de novo synthesis of tetrahydrobiopterin (BH4), an essential cofactor for endothelial NO synthase (eNOS) dictating, at least partly, the balance of NO and superoxide produced by this enzyme...
  2. Gava A, Peotta V, Cabral A, Vasquez E, Meyrelles S. Overexpression of eNOS prevents the development of renovascular hypertension in mice. Can J Physiol Pharmacol. 2008;86:458-64 pubmed publisher
    ..In the present study we investigated the effects of endothelial nitric oxide synthase (eNOS) overexpression on renovascular hypertension...
  3. Fisslthaler B, Loot A, Mohamed A, Busse R, Fleming I. Inhibition of endothelial nitric oxide synthase activity by proline-rich tyrosine kinase 2 in response to fluid shear stress and insulin. Circ Res. 2008;102:1520-8 pubmed publisher
    ..of endothelial cells, fluid shear stress elicits the tyrosine phosphorylation of the endothelial NO synthase (eNOS), however, the consequences of this modification on enzyme activity are unclear...
  4. Cabou C, Cani P, Campistron G, Knauf C, Mathieu C, Sartori C, et al. Central insulin regulates heart rate and arterial blood flow: an endothelial nitric oxide synthase-dependent mechanism altered during diabetes. Diabetes. 2007;56:2872-7 pubmed
    ..b>Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) represents a molecular link between metabolic and cardiovascular ..
  5. Fujita Y, Hosokawa M, Fujimoto S, Mukai E, Abudukadier A, Obara A, et al. Metformin suppresses hepatic gluconeogenesis and lowers fasting blood glucose levels through reactive nitrogen species in mice. Diabetologia. 2010;53:1472-81 pubmed publisher
    ..To investigate AMPK activation by metformin in liver, we examined the role of reactive nitrogen species (RNS) in suppression of hepatic gluconeogenesis...
  6. Chang A, Fu Y, Garside V, Niessen K, Chang L, Fuller M, et al. Notch initiates the endothelial-to-mesenchymal transition in the atrioventricular canal through autocrine activation of soluble guanylyl cyclase. Dev Cell. 2011;21:288-300 pubmed publisher
    ..nitric oxide (NO) production by inducing Activin A, thereby activating a PI3-kinase/Akt pathway to phosphorylate eNOS. We thus show that the activation of sGC by NO through a Notch-dependent autocrine loop is necessary to drive early ..
  7. Wang C, Li F, Hiller S, Kim H, Maeda N, Smithies O, et al. A modest decrease in endothelial NOS in mice comparable to that associated with human NOS3 variants exacerbates diabetic nephropathy. Proc Natl Acad Sci U S A. 2011;108:2070-5 pubmed publisher
    Polymorphisms in the human endothelial nitric oxide synthase (eNOS) gene (NOS3) have been associated with advanced nephropathy in diabetic patients and with decreased expression in tissue culture...
  8. Calvert J, Condit M, Aragón J, Nicholson C, Moody B, Hood R, et al. Exercise protects against myocardial ischemia-reperfusion injury via stimulation of ?(3)-adrenergic receptors and increased nitric oxide signaling: role of nitrite and nitrosothiols. Circ Res. 2011;108:1448-58 pubmed publisher
    ..It is still unclear how exercise protects the heart, but it is apparent that endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) play a role...
  9. Yuen D, Stead B, Zhang Y, White K, Kabir M, Thai K, et al. eNOS deficiency predisposes podocytes to injury in diabetes. J Am Soc Nephrol. 2012;23:1810-23 pubmed publisher
    b>Endothelial nitric oxide synthase (eNOS) deficiency may contribute to the pathogenesis of diabetic nephropathy in both experimental models and humans, but the underlying mechanism is not fully understood...
  10. Schoeb T, Jarmi T, Hicks M, Henke S, Zarjou A, Suzuki H, et al. Endothelial nitric oxide synthase inhibits the development of autoimmune-mediated vasculitis in mice. Arthritis Rheum. 2012;64:4114-24 pubmed publisher
    ..b>Endothelial nitric oxide synthase (eNOS) has been shown to inhibit vascular inflammation in many different model systems, but its ..

Detail Information

Publications81

  1. Wang S, Xu J, Song P, Wu Y, Zhang J, Chul Choi H, et al. Acute inhibition of guanosine triphosphate cyclohydrolase 1 uncouples endothelial nitric oxide synthase and elevates blood pressure. Hypertension. 2008;52:484-90 pubmed publisher
    ..enzyme in de novo synthesis of tetrahydrobiopterin (BH4), an essential cofactor for endothelial NO synthase (eNOS) dictating, at least partly, the balance of NO and superoxide produced by this enzyme...
  2. Gava A, Peotta V, Cabral A, Vasquez E, Meyrelles S. Overexpression of eNOS prevents the development of renovascular hypertension in mice. Can J Physiol Pharmacol. 2008;86:458-64 pubmed publisher
    ..In the present study we investigated the effects of endothelial nitric oxide synthase (eNOS) overexpression on renovascular hypertension...
  3. Fisslthaler B, Loot A, Mohamed A, Busse R, Fleming I. Inhibition of endothelial nitric oxide synthase activity by proline-rich tyrosine kinase 2 in response to fluid shear stress and insulin. Circ Res. 2008;102:1520-8 pubmed publisher
    ..of endothelial cells, fluid shear stress elicits the tyrosine phosphorylation of the endothelial NO synthase (eNOS), however, the consequences of this modification on enzyme activity are unclear...
  4. Cabou C, Cani P, Campistron G, Knauf C, Mathieu C, Sartori C, et al. Central insulin regulates heart rate and arterial blood flow: an endothelial nitric oxide synthase-dependent mechanism altered during diabetes. Diabetes. 2007;56:2872-7 pubmed
    ..b>Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) represents a molecular link between metabolic and cardiovascular ..
  5. Fujita Y, Hosokawa M, Fujimoto S, Mukai E, Abudukadier A, Obara A, et al. Metformin suppresses hepatic gluconeogenesis and lowers fasting blood glucose levels through reactive nitrogen species in mice. Diabetologia. 2010;53:1472-81 pubmed publisher
    ..To investigate AMPK activation by metformin in liver, we examined the role of reactive nitrogen species (RNS) in suppression of hepatic gluconeogenesis...
  6. Chang A, Fu Y, Garside V, Niessen K, Chang L, Fuller M, et al. Notch initiates the endothelial-to-mesenchymal transition in the atrioventricular canal through autocrine activation of soluble guanylyl cyclase. Dev Cell. 2011;21:288-300 pubmed publisher
    ..nitric oxide (NO) production by inducing Activin A, thereby activating a PI3-kinase/Akt pathway to phosphorylate eNOS. We thus show that the activation of sGC by NO through a Notch-dependent autocrine loop is necessary to drive early ..
  7. Wang C, Li F, Hiller S, Kim H, Maeda N, Smithies O, et al. A modest decrease in endothelial NOS in mice comparable to that associated with human NOS3 variants exacerbates diabetic nephropathy. Proc Natl Acad Sci U S A. 2011;108:2070-5 pubmed publisher
    Polymorphisms in the human endothelial nitric oxide synthase (eNOS) gene (NOS3) have been associated with advanced nephropathy in diabetic patients and with decreased expression in tissue culture...
  8. Calvert J, Condit M, Aragón J, Nicholson C, Moody B, Hood R, et al. Exercise protects against myocardial ischemia-reperfusion injury via stimulation of ?(3)-adrenergic receptors and increased nitric oxide signaling: role of nitrite and nitrosothiols. Circ Res. 2011;108:1448-58 pubmed publisher
    ..It is still unclear how exercise protects the heart, but it is apparent that endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) play a role...
  9. Yuen D, Stead B, Zhang Y, White K, Kabir M, Thai K, et al. eNOS deficiency predisposes podocytes to injury in diabetes. J Am Soc Nephrol. 2012;23:1810-23 pubmed publisher
    b>Endothelial nitric oxide synthase (eNOS) deficiency may contribute to the pathogenesis of diabetic nephropathy in both experimental models and humans, but the underlying mechanism is not fully understood...
  10. Schoeb T, Jarmi T, Hicks M, Henke S, Zarjou A, Suzuki H, et al. Endothelial nitric oxide synthase inhibits the development of autoimmune-mediated vasculitis in mice. Arthritis Rheum. 2012;64:4114-24 pubmed publisher
    ..b>Endothelial nitric oxide synthase (eNOS) has been shown to inhibit vascular inflammation in many different model systems, but its ..
  11. Crabtree M, Tatham A, Hale A, Alp N, Channon K. Critical role for tetrahydrobiopterin recycling by dihydrofolate reductase in regulation of endothelial nitric-oxide synthase coupling: relative importance of the de novo biopterin synthesis versus salvage pathways. J Biol Chem. 2009;284:28128-36 pubmed publisher
    ..BH4) is a required cofactor for the synthesis of nitric oxide by endothelial nitric-oxide synthase (eNOS), and BH4 bioavailability within the endothelium is a critical factor in regulating the balance between NO and ..
  12. Wu Z, Xiong Y, Gajanayake T, Ming X, Yang Z. p38 Mitogen-activated protein kinase is required for glucosamine-induced endothelial nitric oxide synthase uncoupling and plasminogen-activator inhibitor expression. Circ J. 2012;76:2015-22 pubmed
    ..biosynthetic pathway (HBP) is implicated in increased plasminogen activator inhibitor-1 (PAI-1), and endothelial nitric oxide synthase (eNOS) dysfunction in diabetes...
  13. Musicki B, Champion H, Hsu L, Bivalacqua T, Burnett A. Post-translational inactivation of endothelial nitric oxide synthase in the transgenic sickle cell mouse penis. J Sex Med. 2011;8:419-26 pubmed publisher
    Sickle cell disease (SCD)-associated priapism is characterized by endothelial nitric oxide synthase (eNOS) dysfunction in the penis...
  14. Vaporidi K, Francis R, Bloch K, Zapol W. Nitric oxide synthase 3 contributes to ventilator-induced lung injury. Am J Physiol Lung Cell Mol Physiol. 2010;299:L150-9 pubmed publisher
    ..The aim of this study was to elucidate the role of endothelial NOS, NOS3, in the pathogenesis of VILI in an in vivo mouse model...
  15. Mei Y, Thevananther S. Endothelial nitric oxide synthase is a key mediator of hepatocyte proliferation in response to partial hepatectomy in mice. Hepatology. 2011;54:1777-89 pubmed publisher
    b>Endothelial nitric oxide synthase (eNOS) is a critical modulator of vascular tone and blood flow and plays major roles in liver physiology and pathophysiology...
  16. Bernatchez P, Sharma A, Bauer P, Marin E, Sessa W. A noninhibitory mutant of the caveolin-1 scaffolding domain enhances eNOS-derived NO synthesis and vasodilation in mice. J Clin Invest. 2011;121:3747-55 pubmed publisher
    Aberrant regulation of eNOS and associated NO release are directly linked with various vascular diseases. Caveolin-1 (Cav-1), the main coat protein of caveolae, is highly expressed in endothelial cells...
  17. Siddiqui M, Komarova Y, Vogel S, Gao X, Bonini M, Rajasingh J, et al. Caveolin-1-eNOS signaling promotes p190RhoGAP-A nitration and endothelial permeability. J Cell Biol. 2011;193:841-50 pubmed publisher
    ..cells isolated from Cav-1(-/-) mice, we show that Cav-1 deficiency induced the activation of endothelial nitric oxide synthase (eNOS) and the generation of nitric oxide (NO) and peroxynitrite...
  18. Gao L, Siu K, Chalupsky K, Nguyen A, Chen P, Weintraub N, et al. Role of uncoupled endothelial nitric oxide synthase in abdominal aortic aneurysm formation: treatment with folic acid. Hypertension. 2012;59:158-66 pubmed publisher
    It has been shown that endothelial NO synthase (eNOS) uncoupling occurs in hypertension and atherosclerosis. However, its causal role in vascular pathogenesis has not been characterized previously...
  19. Kulandavelu S, Whiteley K, Qu D, Mu J, Bainbridge S, Adamson S. Endothelial nitric oxide synthase deficiency reduces uterine blood flow, spiral artery elongation, and placental oxygenation in pregnant mice. Hypertension. 2012;60:231-8 pubmed publisher
    ..with impaired uteroplacental adaptations during pregnancy and abnormalities in the endothelial NO synthase (eNOS)-NO pathway, but whether eNOS deficiency plays a causal role is unknown...
  20. Symons J, McMillin S, Riehle C, Tanner J, Palionyte M, Hillas E, et al. Contribution of insulin and Akt1 signaling to endothelial nitric oxide synthase in the regulation of endothelial function and blood pressure. Circ Res. 2009;104:1085-94 pubmed publisher
    Impaired insulin signaling via phosphatidylinositol 3-kinase/Akt to endothelial nitric oxide synthase (eNOS) in the vasculature has been postulated to lead to arterial dysfunction and hypertension in obesity and other insulin resistant ..
  21. Tymvios C, Moore C, Jones S, Solomon A, Sanz Rosa D, Emerson M. Platelet aggregation responses are critically regulated in vivo by endogenous nitric oxide but not by endothelial nitric oxide synthase. Br J Pharmacol. 2009;158:1735-42 pubmed publisher
  22. Moens A, Leyton Mange J, Niu X, Yang R, Cingolani O, Arkenbout E, et al. Adverse ventricular remodeling and exacerbated NOS uncoupling from pressure-overload in mice lacking the beta3-adrenoreceptor. J Mol Cell Cardiol. 2009;47:576-85 pubmed publisher
    ..The level of eNOS phosphorylation in beta3(-/-)TAC hearts was diminished, and nNOS and iNOS expression levels were increased...
  23. Cheng H, Wang H, Fan X, Paueksakon P, Harris R. Improvement of endothelial nitric oxide synthase activity retards the progression of diabetic nephropathy in db/db mice. Kidney Int. 2012;82:1176-83 pubmed publisher
    Impaired endothelial nitric oxide synthase (eNOS) activity may be involved in the pathogenesis of diabetic nephropathy...
  24. Takaki A, Morikawa K, Tsutsui M, Murayama Y, Tekes E, Yamagishi H, et al. Crucial role of nitric oxide synthases system in endothelium-dependent hyperpolarization in mice. J Exp Med. 2008;205:2053-63 pubmed publisher
    ..endothelium-derived hydrogen peroxide (H(2)O(2)) is an EDHF that is produced in part by endothelial NO synthase (eNOS)...
  25. Gentile C, Muise Helmericks R, Drake C. VEGF-mediated phosphorylation of eNOS regulates angioblast and embryonic endothelial cell proliferation. Dev Biol. 2013;373:163-75 pubmed publisher
    ..of the endothelial lineage and neovascular processes (vasculogenesis and angiogenesis) we evaluated endothelial nitric oxide synthase (eNOS) and phosphorylated eNOS (p-eNOS) expression in 7.2-8.5 days post-coitum (dpc) mouse embryos...
  26. Sun Y, Qu X, Zhang X, Caruana G, Bertram J, Li J. Glomerular endothelial cell injury and damage precedes that of podocytes in adriamycin-induced nephropathy. PLoS ONE. 2013;8:e55027 pubmed publisher
    ..Recent studies have demonstrated that endothelial nitric oxide synthatase (eNOS) deficiency exacerbates renal injury in anti-GBM and remnant kidney models and accelerates diabetic kidney damage...
  27. Rizzo N, Maloney E, Pham M, Luttrell I, Wessells H, Tateya S, et al. Reduced NO-cGMP signaling contributes to vascular inflammation and insulin resistance induced by high-fat feeding. Arterioscler Thromb Vasc Biol. 2010;30:758-65 pubmed publisher
    ..and intercellular adhesion molecule levels) and insulin resistance (phosphorylated Akt [pAkt] and phosphorylated eNOS [peNOS] levels) were increased in endothelial NO synthase(-/-) (eNOS(-/-)) mice, reminiscent of the effect of DIO ..
  28. Nakayama T, Sato W, Yoshimura A, Zhang L, Kosugi T, Campbell Thompson M, et al. Endothelial von Willebrand factor release due to eNOS deficiency predisposes to thrombotic microangiopathy in mouse aging kidney. Am J Pathol. 2010;176:2198-208 pubmed publisher
    Endothelial dysfunction is critical in the decline of renal function with. By using endothelial nitric oxide synthase knockout (eNOSKO) mice, we tested the hypothesis that a lack of endothelial nitric oxide synthase accelerates renal ..
  29. Nakajima S, Ohashi J, Sawada A, Noda K, Fukumoto Y, Shimokawa H. Essential role of bone marrow for microvascular endothelial and metabolic functions in mice. Circ Res. 2012;111:87-96 pubmed publisher
    ..that endothelium-derived hydrogen peroxide (H(2)O(2)) is an EDHF in animals and humans, for which endothelial nitric oxide synthase (eNOS) is the major source...
  30. Li Q, Verma A, Han P, Nakagawa T, Johnson R, Grant M, et al. Diabetic eNOS-knockout mice develop accelerated retinopathy. Invest Ophthalmol Vis Sci. 2010;51:5240-6 pubmed publisher
    Dysfunction of endothelial nitric oxide synthase (eNOS) has been implicated in the pathogenesis of diabetic vascular complications...
  31. Kashiwagi S, Atochin D, Li Q, Schleicher M, Pong T, Sessa W, et al. eNOS phosphorylation on serine 1176 affects insulin sensitivity and adiposity. Biochem Biophys Res Commun. 2013;431:284-90 pubmed publisher
    Phosphorylation of endothelial nitric oxide synthase (eNOS) is an important regulator of its enzymatic activity...
  32. Yang Y, Huang A, Kaley G, Sun D. eNOS uncoupling and endothelial dysfunction in aged vessels. Am J Physiol Heart Circ Physiol. 2009;297:H1829-36 pubmed publisher
    b>Endothelial nitric oxide synthase (eNOS) uncoupling is a mechanism that leads to endothelial dysfunction...
  33. Schleicher M, Yu J, Murata T, Derakhshan B, Atochin D, Qian L, et al. The Akt1-eNOS axis illustrates the specificity of kinase-substrate relationships in vivo. Sci Signal. 2009;2:ra41 pubmed publisher
    ..Here, we examine the importance of endothelial nitric oxide synthase (eNOS) as an Akt1 substrate by generating Akt1-deficient mice (Akt1(-/-) mice) carrying knock-in ..
  34. Kusinski L, Stanley J, Dilworth M, Hirt C, Andersson I, Renshall L, et al. eNOS knockout mouse as a model of fetal growth restriction with an impaired uterine artery function and placental transport phenotype. Am J Physiol Regul Integr Comp Physiol. 2012;303:R86-93 pubmed publisher
    ..Pregnant endothelial nitric oxide synthase knockout (eNOS(-/-)) dams exhibit dysregulated vascular adaptations to pregnancy, and eNOS(-/-) ..
  35. Zhao Y, Zhao Y, Mirza M, Huang J, Potula H, Vogel S, et al. Persistent eNOS activation secondary to caveolin-1 deficiency induces pulmonary hypertension in mice and humans through PKG nitration. J Clin Invest. 2009;119:2009-18 pubmed publisher
    ..Using mice with genetic deletions of caveolin 1 (Cav1) and eNOS (Nos3), we demonstrate here that chronic eNOS activation secondary to loss of caveolin-1 can lead to PH...
  36. Cui X, Chopp M, Zacharek A, Zhang C, Roberts C, Chen J. Role of endothelial nitric oxide synthetase in arteriogenesis after stroke in mice. Neuroscience. 2009;159:744-50 pubmed publisher
    ..We investigate the role of endothelial nitric oxide synthetase (eNOS) and a nitric oxide (NO) donor, (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl) amino] diazen-1-ium-1, 2-diolate (DETA-..
  37. Nakayama T, Sato W, Kosugi T, Zhang L, Campbell Thompson M, Yoshimura A, et al. Endothelial injury due to eNOS deficiency accelerates the progression of chronic renal disease in the mouse. Am J Physiol Renal Physiol. 2009;296:F317-27 pubmed publisher
    The vascular endothelium expresses endothelial nitric oxide synthase (eNOS) that generates nitric oxide (NO) to help maintain vascular integrity due to its anti-inflammatory, antiproliferative, and antithrombogenic effects...
  38. Crabtree M, Tatham A, Al Wakeel Y, Warrick N, Hale A, Cai S, et al. Quantitative regulation of intracellular endothelial nitric-oxide synthase (eNOS) coupling by both tetrahydrobiopterin-eNOS stoichiometry and biopterin redox status: insights from cells with tet-regulated GTP cyclohydrolase I expression. J Biol Chem. 2009;284:1136-44 pubmed publisher
    Tetrahydrobiopterin (BH4) is a critical determinant of endothelial nitric-oxide synthase (eNOS) activity. In the absence of BH4, eNOS becomes "uncoupled" and generates superoxide rather than NO...
  39. Teichert A, Scott J, Robb G, Zhou Y, Zhu S, Lem M, et al. Endothelial nitric oxide synthase gene expression during murine embryogenesis: commencement of expression in the embryo occurs with the establishment of a unidirectional circulatory system. Circ Res. 2008;103:24-33 pubmed publisher
    To elucidate the role of endothelial NO synthase (eNOS)-derived NO during mammalian embryogenesis, we assessed the expression of the eNOS gene during development...
  40. Thibeault S, Rautureau Y, Oubaha M, Faubert D, Wilkes B, Delisle C, et al. S-nitrosylation of beta-catenin by eNOS-derived NO promotes VEGF-induced endothelial cell permeability. Mol Cell. 2010;39:468-76 pubmed publisher
    ..Nitric oxide (NO) produced by endothelial NO synthase (eNOS) is essential for increased vascular permeability induced by vascular endothelial growth factor (VEGF)...
  41. Maruyama S, Shibata R, Kikuchi R, Izumiya Y, Rokutanda T, Araki S, et al. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism. J Biol Chem. 2012;287:408-17 pubmed publisher
    ..limbs of wild-type mice in vivo, which were accompanied by increased phosphorylation of Akt and endothelial nitric oxide synthase (eNOS)...
  42. Ota H, Akishita M, Akiyoshi T, Kahyo T, Setou M, Ogawa S, et al. Testosterone deficiency accelerates neuronal and vascular aging of SAMP8 mice: protective role of eNOS and SIRT1. PLoS ONE. 2012;7:e29598 pubmed publisher
    ..Testosterone increased eNOS activity and subsequently induced SIRT1 expression...
  43. Han R, Babaei S, Robb M, Lee T, Ridsdale R, Ackerley C, et al. Defective lung vascular development and fatal respiratory distress in endothelial NO synthase-deficient mice: a model of alveolar capillary dysplasia?. Circ Res. 2004;94:1115-23 pubmed
    ..Although endothelial NO synthase (eNOS)-deficient mice are viable, minor congenital cardiac abnormalities have been reported and homozygous offspring ..
  44. Lefer D, Jones S, Girod W, Baines A, Grisham M, Cockrell A, et al. Leukocyte-endothelial cell interactions in nitric oxide synthase-deficient mice. Am J Physiol. 1999;276:H1943-50 pubmed
    ..mesentery of wild-type, inducible NOS (iNOS)-deficient (-/-), neuronal NOS (nNOS) -/-, and endothelial cell NOS (ecNOS) -/- mice to further our understanding of NO and leukocyte function...
  45. Miller A, Hislop A, Vallance P, Haworth S. Deletion of the eNOS gene has a greater impact on the pulmonary circulation of male than female mice. Am J Physiol Lung Cell Mol Physiol. 2005;289:L299-306 pubmed
    ..Morphometric analyses were made on lung tissue from male and female fetal, newborn, 14-day-old, and adult endothelial NOS-deficient (eNOS-/-) or inducible NOS-deficient (iNOS-/-) and wild-type mice...
  46. Patzak A, Steege A, Lai E, Brinkmann J, Kupsch E, Spielmann N, et al. Angiotensin II response in afferent arterioles of mice lacking either the endothelial or neuronal isoform of nitric oxide synthase. Am J Physiol Regul Integr Comp Physiol. 2008;294:R429-37 pubmed
    The aim of the study is to evaluate the impact of nitric oxide (NO) produced by endothelial NO synthase (eNOS) and neuronal NOS (nNOS) on the angiotensin II response in afferent arterioles (Af)...
  47. Lamping K, Nuno D, Shesely E, Maeda N, Faraci F. Vasodilator mechanisms in the coronary circulation of endothelial nitric oxide synthase-deficient mice. Am J Physiol Heart Circ Physiol. 2000;279:H1906-12 pubmed
    ..to endothelium-dependent vasodilators are absent in the aortas from mice deficient in expression of endothelial nitric oxide synthase (eNOS -/- mice), whereas responses in the cerebral microcirculation are preserved...
  48. De Sanctis G, MacLean J, Hamada K, Mehta S, Scott J, Jiao A, et al. Contribution of nitric oxide synthases 1, 2, and 3 to airway hyperresponsiveness and inflammation in a murine model of asthma. J Exp Med. 1999;189:1621-30 pubmed
    ..OVA/OVA endothelial (NOS3)-deficient mice were significantly more responsive to methacholine challenge compared with similarly treated NOS1 ..
  49. Xu J, Xie Z, Reece R, Pimental D, Zou M. Uncoupling of endothelial nitric oxidase synthase by hypochlorous acid: role of NAD(P)H oxidase-derived superoxide and peroxynitrite. Arterioscler Thromb Vasc Biol. 2006;26:2688-95 pubmed
    ..the major oxidant of leukocyte-derived myeloperoxidase (MPO), oxidizes the zinc-thiolate center of endothelial nitric oxide synthase (eNOS) and uncouples the enzyme...
  50. Borniquel S, Valle I, Cadenas S, Lamas S, Monsalve M. Nitric oxide regulates mitochondrial oxidative stress protection via the transcriptional coactivator PGC-1alpha. FASEB J. 2006;20:1889-91 pubmed
    ..Analysis of tissues from eNOS(-/-) mice showed reduced levels of PGC-1alpha and the mitochondrial ROS detoxification system...
  51. Yu J, Demuinck E, Zhuang Z, Drinane M, Kauser K, Rubanyi G, et al. Endothelial nitric oxide synthase is critical for ischemic remodeling, mural cell recruitment, and blood flow reserve. Proc Natl Acad Sci U S A. 2005;102:10999-1004 pubmed
    The genetic loss of endothelial-derived nitric oxide synthase (eNOS) in mice impairs vascular endothelial growth factor (VEGF) and ischemia-initiated blood flow recovery resulting in critical limb ischemia...
  52. Kanasaki K, Palmsten K, Sugimoto H, Ahmad S, Hamano Y, Xie L, et al. Deficiency in catechol-O-methyltransferase and 2-methoxyoestradiol is associated with pre-eclampsia. Nature. 2008;453:1117-21 pubmed publisher
  53. Kuhlencordt P, Rosel E, Gerszten R, Morales Ruiz M, Dombkowski D, Atkinson W, et al. Role of endothelial nitric oxide synthase in endothelial activation: insights from eNOS knockout endothelial cells. Am J Physiol Cell Physiol. 2004;286:C1195-202 pubmed
    The objective of this study was to determine whether absence of endothelial nitric oxide synthase (eNOS) affects the expression of cell surface adhesion molecules in endothelial cells...
  54. Sharp B, Jones S, Rimmer D, Lefer D. Differential response to myocardial reperfusion injury in eNOS-deficient mice. Am J Physiol Heart Circ Physiol. 2002;282:H2422-6 pubmed
    Two strains of endothelial nitric oxide synthase (eNOS)-deficient (-/-) mice have been developed that respond differently to myocardial ischemia-reperfusion (MI/R). We evaluated both strains of eNOS(-/-) mice in an in vivo model of MI/R...
  55. Burnett A, Chang A, Crone J, Huang P, Sezen S. Noncholinergic penile erection in mice lacking the gene for endothelial nitric oxide synthase. J Androl. 2002;23:92-7 pubmed
    ..the current understanding that nitric oxide (NO) mediates penile erection, the endothelial isoform of NO synthase (eNOS) has been implicated in this function...
  56. Ye Y, Martinez J, Perez Polo R, Lin Y, Uretsky B, Birnbaum Y. The role of eNOS, iNOS, and NF-kappaB in upregulation and activation of cyclooxygenase-2 and infarct size reduction by atorvastatin. Am J Physiol Heart Circ Physiol. 2008;295:H343-51 pubmed publisher
    ..atorvastatin (ATV) reduces infarct size (IS) and increases myocardial expression of phosphorylated endothelial nitric oxide synthase (p-eNOS), inducible NOS (iNOS), and cyclooxygenase-2 (COX2) in the rat...
  57. Arnhold S, Andressen C, Bloch W, Mai J, Addicks K. NO synthase-II is transiently expressed in embryonic mouse olfactory receptor neurons. Neurosci Lett. 1997;229:165-8 pubmed
    ..This transient expression of NOS-II implies a role for the differentiation of early olfactory receptor neurons and synaptic plasticity. ..
  58. Michel T, Feron O. Nitric oxide synthases: which, where, how, and why?. J Clin Invest. 1997;100:2146-52 pubmed
  59. Linares D, Taconis M, Maña P, Correcha M, Fordham S, Staykova M, et al. Neuronal nitric oxide synthase plays a key role in CNS demyelination. J Neurosci. 2006;26:12672-81 pubmed
    ..However, recent reports have suggested that the constitutive isoforms of NOS, neuronal (nNOS) and endothelial (eNOS), can also play a role...
  60. DiMagno M, Hao Y, Tsunoda Y, Williams J, Owyang C. Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice. Am J Physiol Gastrointest Liver Physiol. 2004;286:G428-36 pubmed
    ..NOS blockade by L-NNA had no effect on carbachol-stimulated amylase secretion in vitro. Thus constitutive NOS isoforms can exert opposite effects on in vivo pancreatic secretion...
  61. Shesely E, Gilbert C, Granderson G, Carretero C, Carretero O, Beierwaltes W. Nitric oxide synthase gene knockout mice do not become hypertensive during pregnancy. Am J Obstet Gynecol. 2001;185:1198-203 pubmed
    ..This suggests that, at least in the mouse, genetic deficiency of any 1 isoform of nitric oxide synthase does not result in pregnancy-induced hypertension. ..
  62. Shi W, Wang X, Shih D, Laubach V, Navab M, Lusis A. Paradoxical reduction of fatty streak formation in mice lacking endothelial nitric oxide synthase. Circulation. 2002;105:2078-82 pubmed
    The endothelial isoform of nitric oxide synthase (eNOS) has been considered to exert an antiatherosclerotic role through synthesis of NO...
  63. Mattagajasingh I, Kim C, Naqvi A, Yamamori T, Hoffman T, Jung S, et al. SIRT1 promotes endothelium-dependent vascular relaxation by activating endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 2007;104:14855-60 pubmed
    ..Here we show that SIRT1 promotes endothelium-dependent vasodilation by targeting endothelial nitric oxide synthase (eNOS) for deacetylation...
  64. Nakagawa T, Sato W, Glushakova O, Heinig M, Clarke T, Campbell Thompson M, et al. Diabetic endothelial nitric oxide synthase knockout mice develop advanced diabetic nephropathy. J Am Soc Nephrol. 2007;18:539-50 pubmed
    ..For testing of this hypothesis, diabetes was induced in eNO synthase knockout mice (eNOS KO) and C57BL6 controls...
  65. Billon A, Lehoux S, Lam Shang Leen L, Laurell H, Filipe C, Benouaich V, et al. The estrogen effects on endothelial repair and mitogen-activated protein kinase activation are abolished in endothelial nitric-oxide (NO) synthase knockout mice, but not by NO synthase inhibition by N-nitro-L-arginine methyl ester. Am J Pathol. 2008;172:830-8 pubmed publisher
    ..of nitric oxide, in this study, we used the same perivascular model to characterize the role of the endothelial nitric oxide synthase (eNOS) pathway in reendothelialization...
  66. Boettger M, Uceyler N, Zelenka M, Schmitt A, Reif A, Chen Y, et al. Differences in inflammatory pain in nNOS-, iNOS- and eNOS-deficient mice. Eur J Pain. 2007;11:810-8 pubmed
    ..complete Freund's adjuvant (CFA) in wild-type (WT) mice and in mice lacking either inducible (iNOS), endothelial (eNOS) or neuronal NOS (nNOS)...
  67. Steudel W, Kirmse M, Weimann J, Ullrich R, Hromi J, Zapol W. Exhaled nitric oxide production by nitric oxide synthase-deficient mice. Am J Respir Crit Care Med. 2000;162:1262-7 pubmed
    ..We therefore studied wild-type mice and mice with a congenital deficiency of NOS 1, NOS 2, or NOS 3. By studying a closed chamber in which the exhaled gas of a group of mice was collected, gaseous NO production ..
  68. Heeringa P, van Goor H, Itoh Lindstrom Y, Maeda N, Falk R, Assmann K, et al. Lack of endothelial nitric oxide synthase aggravates murine accelerated anti-glomerular basement membrane glomerulonephritis. Am J Pathol. 2000;156:879-88 pubmed
    Nitric oxide (NO) radicals generated by endothelial nitric oxide synthase (eNOS) are involved in the regulation of vascular tone...
  69. Feng Q, Song W, Lu X, Hamilton J, Lei M, Peng T, et al. Development of heart failure and congenital septal defects in mice lacking endothelial nitric oxide synthase. Circulation. 2002;106:873-9 pubmed
    Nitric oxide (NO) produced by endothelial NO synthase (eNOS) plays an important role in the regulation of cell growth, apoptosis, and tissue perfusion...
  70. Lee J, Yu Q, Shin J, Sebzda E, Bertozzi C, Chen M, et al. Klf2 is an essential regulator of vascular hemodynamic forces in vivo. Dev Cell. 2006;11:845-57 pubmed
    ..These findings identify Klf2 as an essential hemodynamic regulator in vivo and suggest that hemodynamic regulation in response to fluid shear stress is required for cardiovascular development and function. ..
  71. Chen J, Zacharek A, Zhang C, Jiang H, Li Y, Roberts C, et al. Endothelial nitric oxide synthase regulates brain-derived neurotrophic factor expression and neurogenesis after stroke in mice. J Neurosci. 2005;25:2366-75 pubmed
    Here, we investigate the effects of endothelial nitric oxide synthase (eNOS) on angiogenesis, neurogenesis, neurotrophic factor expression, and neurological functional outcome after stroke...
  72. Dixit M, Loot A, Mohamed A, Fisslthaler B, Boulanger C, Ceacareanu B, et al. Gab1, SHP2, and protein kinase A are crucial for the activation of the endothelial NO synthase by fluid shear stress. Circ Res. 2005;97:1236-44 pubmed
    ..the activation of the phosphatidylinositol 3-kinase and the phosphorylation of the endothelial NO synthase (eNOS)...