Gene Symbol: Nono
Description: non-POU-domain-containing, octamer binding protein
Alias: AA407051, AV149256, NRB54, P54NRB, nonA, non-POU domain-containing octamer-binding protein
Species: mouse
Products:     Nono

Top Publications

  1. Kowalska E, Ripperger J, Hoegger D, Bruegger P, Buch T, Birchler T, et al. NONO couples the circadian clock to the cell cycle. Proc Natl Acad Sci U S A. 2013;110:1592-9 pubmed publisher
    ..Previously we identified the multifunctional nuclear protein NONO as a partner of circadian PERIOD (PER) proteins...
  2. Kuwahara S, Ikei A, Taguchi Y, Tabuchi Y, Fujimoto N, Obinata M, et al. PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription. Biol Reprod. 2006;75:352-9 pubmed
    ..Non-POU-domain-containing, octamer binding protein (NONO), a member of the DBHS-containing proteins, complexes with androgen receptor and functions as a coactivator for the ..
  3. Yang Y, Hanke J, Carayannopoulos L, Craft C, Capra J, Tucker P. NonO, a non-POU-domain-containing, octamer-binding protein, is the mammalian homolog of Drosophila nonAdiss. Mol Cell Biol. 1993;13:5593-603 pubmed
    We have cloned the ubiquitous form of an octamer-binding, 60-kDa protein (NonO) that appears to be the mammalian equivalent of the Drosophila visual and courtship song behavior protein, no-on-transient A/dissonance (nonAdiss)...
  4. Karhumaa P, Parkkila S, Waheed A, Parkkila A, Kaunisto K, Tucker P, et al. Nuclear NonO/p54(nrb) protein is a nonclassical carbonic anhydrase. J Biol Chem. 2000;275:16044-9 pubmed
    ..It appeared to be identical to nonO/p54(nrb), a previously cloned and characterized RNA and DNA binding nuclear factor...
  5. Hallier M, Tavitian A, Moreau Gachelin F. The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb. J Biol Chem. 1996;271:11177-81 pubmed
    ..1 protein. Microsequence analysis revealed that this 55-kDa protein was p54nrb (nuclear RNA-binding protein, 54 kDa) a RNA-binding protein highly similar to the splicing factor PSF (..
  6. Duvignaud J, Bédard M, Nagata T, Muto Y, Yokoyama S, Gagné S, et al. Structure, Dynamics, and Interaction of p54(nrb)/NonO RRM1 with 5' Splice Site RNA Sequence. Biochemistry. 2016;55:2553-66 pubmed publisher
    p54(nrb)/NonO is a nuclear RNA-binding protein involved in many cellular events such as pre-mRNA processing, transcription, and nuclear retention of hyper-edited RNAs...
  7. Kowalska E, Ripperger J, Muheim C, Maier B, Kurihara Y, Fox A, et al. Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Mol Cell Biol. 2012;32:4585-94 pubmed publisher
    ..Taken together, our data suggest that all of the DBHS family members serve overlapping or redundant roles as transcriptional cofactors at circadian clock-regulated genes. ..
  8. Bianconcini A, Lupo A, Capone S, Quadro L, Monti M, Zurlo D, et al. Transcriptional activity of the murine retinol-binding protein gene is regulated by a multiprotein complex containing HMGA1, p54 nrb/NonO, protein-associated splicing factor (PSF) and steroidogenic factor 1 (SF1)/liver receptor homologue 1 (LRH-1). Int J Biochem Cell Biol. 2009;41:2189-203 pubmed publisher
    ..HMGA1 acts to recruit transcription factors to the RBP4 promoter and we specifically identified p54(nrb)/NonO and protein-associated splicing factor (PSF) as components that interact with this complex...
  9. Proteau A, Blier S, Albert A, Lavoie S, Traish A, Vincent M. The multifunctional nuclear protein p54nrb is multiphosphorylated in mitosis and interacts with the mitotic regulator Pin1. J Mol Biol. 2005;346:1163-72 pubmed
    The human protein p54nrb and its mouse homolog NonO have been implicated in a variety of nuclear processes including transcription, pre-mRNA processing, nuclear retention of edited RNA and DNA relaxation...

More Information


  1. Chen N, Onisko B, Napoli J. The nuclear transcription factor RARalpha associates with neuronal RNA granules and suppresses translation. J Biol Chem. 2008;283:20841-7 pubmed publisher
    ..These data afford a mechanism for rapid stimulation of dendritic growth by all-trans-retinoic acid and reveal that the ligand-dependent transcription factor RARalpha also regulates translation. ..
  2. Hata K, Nishimura R, Muramatsu S, Matsuda A, Matsubara T, Amano K, et al. Paraspeckle protein p54nrb links Sox9-mediated transcription with RNA processing during chondrogenesis in mice. J Clin Invest. 2008;118:3098-108 pubmed publisher
    ..Here we have shown that paraspeckle regulatory protein 54-kDa nuclear RNA-binding protein (p54nrb) is an essential link between Sox9-regulated transcription and maturation of Sox9-target gene mRNA...
  3. Dong B, Jin X, Yan C, Yang T, Cai G, Lu J. Synergistic upregulation of NONO and PSPC1 regulates Sertoli cell response to MEHP via modulation of ALDH1A1 signaling. FEBS Lett. 2017;591:914-923 pubmed publisher, including splicing factor proline/glutamine rich (SFPQ), non-POU domain-containing octamer-binding protein (NONO), and paraspeckle protein component 1 (PSPC1), are abundantly expressed in testicular Sertoli cells (SCs), but ..
  4. Clemson C, Hutchinson J, Sara S, Ensminger A, Fox A, Chess A, et al. An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles. Mol Cell. 2009;33:717-26 pubmed publisher
    ..Collectively, results demonstrate that NEAT1 functions as an essential structural determinant of paraspeckles, providing a precedent for a ncRNA as the foundation of a nuclear domain...
  5. Wilhelmsen K, Copp J, Glenn G, Hoffman R, Tucker P, van der Geer P. Purification and identification of protein-tyrosine kinase-binding proteins using synthetic phosphopeptides as affinity reagents. Mol Cell Proteomics. 2004;3:887-95 pubmed
    ..Novel protein-protein interactions may provide useful targets for the development of drugs that interfere with the activation of signaling cascades used by protein-tyrosine kinases to turn on cell division. ..
  6. Duong H, Robles M, Knutti D, Weitz C. A molecular mechanism for circadian clock negative feedback. Science. 2011;332:1436-9 pubmed publisher
    ..These findings provide a function for the PER complex and a molecular mechanism for circadian clock negative feedback. ..
  7. Yang X, Lin L, Zhang X, Ji Y, Lv J, Zhu Y, et al. Identification of a novel repressor element in the cyclo-oxygenase-2 promoter and its nuclear binding protein. Clin Exp Pharmacol Physiol. 2008;35:1204-8 pubmed publisher
    ..extracts bound to this element, one of which was identified as non-POU-domain-containing, octamer-binding protein (NonO)...
  8. Basu A, Dong B, Krainer A, Howe C. The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Mol Cell Biol. 1997;17:677-86 pubmed
    ..the purified calf IPEB, we have cloned a 420-bp cDNA and showed that the encoded protein is the homolog of human p54nrb and mouse NonO, which are characterized by the presence of two RNA recognition motifs...
  9. Sury M, McShane E, Hernandez Miranda L, Birchmeier C, Selbach M. Quantitative proteomics reveals dynamic interaction of c-Jun N-terminal kinase (JNK) with RNA transport granule proteins splicing factor proline- and glutamine-rich (Sfpq) and non-POU domain-containing octamer-binding protein (Nono) during neuronal d. Mol Cell Proteomics. 2015;14:50-65 pubmed publisher
    ..Intriguingly, we found that JNK interacted with neuronal transport granule proteins such as Sfpq and Nono upon NGF treatment...
  10. Nakagawa S, Ip J, Shioi G, Tripathi V, Zong X, Hirose T, et al. Malat1 is not an essential component of nuclear speckles in mice. RNA. 2012;18:1487-99 pubmed publisher
    ..We propose that Malat1 is not essential in living mice maintained under normal laboratory conditions and that its function becomes apparent only in specific cell types and under particular conditions. ..
  11. Ma C, Karwacki Neisius V, Tang H, Li W, Shi Z, Hu H, et al. Nono, a Bivalent Domain Factor, Regulates Erk Signaling and Mouse Embryonic Stem Cell Pluripotency. Cell Rep. 2016;17:997-1007 pubmed publisher
    b>Nono is a component of the para-speckle, which stores and processes RNA. Mouse embryonic stem cells (mESCs) lack para-speckles, leaving the function of Nono in mESCs unclear...
  12. Yadav S, Hao H, Yang H, Kautzmann M, Brooks M, Nellissery J, et al. The transcription-splicing protein NonO/p54nrb and three NonO-interacting proteins bind to distal enhancer region and augment rhodopsin expression. Hum Mol Genet. 2014;23:2132-44 pubmed publisher
    ..Here, we report the identification of RER-bound proteins by mass spectrometry. We validate the binding of NonO (p54(nrb)), a protein implicated in coupling transcription to splicing, and three NonO-interacting proteins-hnRNP M,..
  13. Mircsof D, Langouët M, Rio M, Moutton S, Siquier Pernet K, Bole Feysot C, et al. Mutations in NONO lead to syndromic intellectual disability and inhibitory synaptic defects. Nat Neurosci. 2015;18:1731-6 pubmed publisher
    The NONO protein has been characterized as an important transcriptional regulator in diverse cellular contexts...
  14. Li S, Li Z, Shu F, Xiong H, Phillips A, Dynan W. Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog. Nucleic Acids Res. 2014;42:9771-80 pubmed publisher
    b>NONO, SFPQ and PSPC1 make up a family of proteins with diverse roles in transcription, RNA processing and DNA double-strand break (DSB) repair...
  15. Mathur M, Tucker P, Samuels H. PSF is a novel corepressor that mediates its effect through Sin3A and the DNA binding domain of nuclear hormone receptors. Mol Cell Biol. 2001;21:2298-311 pubmed
    ..with two proteins which we identified as PSF (polypyrimidine tract-binding protein-associated splicing factor) and NonO/p54(nrb)...
  16. Brown S, Ripperger J, Kadener S, Fleury Olela F, Vilbois F, Rosbash M, et al. PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Science. 2005;308:693-6 pubmed
    ..We identified two PER1-associated factors, NONO and WDR5, that modulate PER activity...
  17. Cambray S, Pedraza N, Rafel M, Garí E, Aldea M, Gallego C. Protein kinase KIS localizes to RNA granules and enhances local translation. Mol Cell Biol. 2009;29:726-35 pubmed publisher
    ..related to microtubule dynamics and axon development, interacts with three proteins found in RNA granules: KIF3A, NonO, and eEF1A...
  18. Furukawa M, Sakamoto H, Inoue K. Interaction and colocalization of HERMES/RBPMS with NonO, PSF, and G3BP1 in neuronal cytoplasmic RNP granules in mouse retinal line cells. Genes Cells. 2015;20:257-66 pubmed publisher
    ..Here, we identified NonO and PSF as the interacting proteins of HERMES only when the neuronal differentiation of the retinal cell line RGC-5 ..
  19. Rousseau S, Morrice N, Peggie M, Campbell D, Gaestel M, Cohen P. Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs. EMBO J. 2002;21:6505-14 pubmed
    ..Our data suggest that LPS-induced binding of hnRNP A0 to AREs may contribute to the post-transcriptional regulation of specific mRNAs. ..
  20. Li S, Shu F, Li Z, Jaafar L, Zhao S, Dynan W. Cell-type specific role of the RNA-binding protein, NONO, in the DNA double-strand break response in the mouse testes. DNA Repair (Amst). 2017;51:70-78 pubmed publisher
    The tandem RNA recognition motif protein, NONO, was previously identified as a candidate DNA double-strand break (DSB) repair factor in a biochemical screen for proteins with end-joining stimulatory activity...
  21. Desai S, Kharade S, Parekh V, Iyer S, Agarwal S. Pro-oncogenic Roles of HLXB9 Protein in Insulinoma Cells through Interaction with Nono Protein and Down-regulation of the c-Met Inhibitor Cblb (Casitas B-lineage Lymphoma b). J Biol Chem. 2015;290:25595-608 pubmed publisher
    ..coupled with mass spectrometry showed a significant association of phospho-HLXB9 with the survival factor p54nrb/Nono (54-kDa nuclear RNA-binding protein, non-POU-domain-containing octamer)...
  22. Xu K, Yang L, Zhao D, Wu Y, Qi H. AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during mouse spermiogenesis. Biol Reprod. 2014;90:119 pubmed publisher
    ..AKAP3 was found to form protein complex with PKA and RNA binding proteins (RBPs), including PIWIL1, PABPC1, and NONO, as revealed by coimmunoprecipitation and protein mass spectrometry...