Gene Symbol: Nfasc
Description: neurofascin
Alias: AA387016, D430023G06Rik, mKIAA0756, neurofascin
Species: mouse
Products:     Nfasc

Top Publications

  1. Zonta B, Tait S, Melrose S, Anderson H, Harroch S, Higginson J, et al. Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system. J Cell Biol. 2008;181:1169-77 pubmed publisher
    ..However, the molecular mechanisms for nodal complex assembly in the CNS are unknown. Two isoforms of Neurofascin, neuronal Nfasc186 and glial Nfasc155, are components of the nodal and paranodal complexes, respectively...
  2. Feinberg K, Eshed Eisenbach Y, Frechter S, Amor V, Salomon D, Sabanay H, et al. A glial signal consisting of gliomedin and NrCAM clusters axonal Na+ channels during the formation of nodes of Ranvier. Neuron. 2010;65:490-502 pubmed publisher
    ..clustering of Na(+) channels at heminodes requires glial NrCAM and gliomedin, as well as their axonal receptor neurofascin 186 (NF186)...
  3. Charles P, Tait S, Faivre Sarrailh C, Barbin G, Gunn Moore F, Denisenko Nehrbass N, et al. Neurofascin is a glial receptor for the paranodin/Caspr-contactin axonal complex at the axoglial junction. Curr Biol. 2002;12:217-20 pubmed
    ..There has been intense speculation that an oligodendroglial isoform of the cell adhesion molecule neurofascin, NF155, expressed at the paranodal loop might be the glial receptor for the paranodin/Caspr-contactin complex, ..
  4. Kim H, DiBernardo A, Sloane J, Rasband M, Solomon D, Kosaras B, et al. WAVE1 is required for oligodendrocyte morphogenesis and normal CNS myelination. J Neurosci. 2006;26:5849-59 pubmed
    ..Together, these data demonstrate a role for WAVE1 in oligodendrocyte morphogenesis and myelination. ..
  5. Ango F, Di Cristo G, Higashiyama H, Bennett V, Wu P, Huang Z. Ankyrin-based subcellular gradient of neurofascin, an immunoglobulin family protein, directs GABAergic innervation at purkinje axon initial segment. Cell. 2004;119:257-72 pubmed
    ..In the absence of neurofascin gradient, basket axons lost directional growth along Purkinje neurons and precisely followed NF186 to ectopic ..
  6. Zonta B, Desmazieres A, Rinaldi A, Tait S, Sherman D, Nolan M, et al. A critical role for Neurofascin in regulating action potential initiation through maintenance of the axon initial segment. Neuron. 2011;69:945-56 pubmed publisher
    ..The neuronal isoform of Neurofascin, Nfasc186, clusters voltage-gated sodium channels at nodes of Ranvier in myelinated nerves: here, we ..
  7. Sherman D, Tait S, Melrose S, Johnson R, Zonta B, Court F, et al. Neurofascins are required to establish axonal domains for saltatory conduction. Neuron. 2005;48:737-42 pubmed
    ..Here, we show that two isoforms of Neurofascin, Nfasc155 in glia and Nfasc186 in neurons, are required for the assembly of these specialized domains...
  8. Brügger V, Engler S, Pereira J, Ruff S, Horn M, Welzl H, et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol. 2015;13:e1002258 pubmed publisher gene myelin protein zero (P0) were reduced by half, accompanied by altered localization and stability of neurofascin (NFasc)155, NFasc186, and loss of Caspr and septate-like junctions...
  9. Williams S, Grumet M, Colman D, Henkemeyer M, Mason C, Sakurai T. A role for Nr-CAM in the patterning of binocular visual pathways. Neuron. 2006;50:535-47 pubmed
    ..Finally, we demonstrate that EphB1/ephrin-B2-mediated repulsion and Nr-CAM-mediated attraction comprise distinct molecular programs that each contributes to the proper formation of binocular visual pathways. ..

More Information


  1. Thaxton C, Pillai A, Pribisko A, Labasque M, Dupree J, Faivre Sarrailh C, et al. In vivo deletion of immunoglobulin domains 5 and 6 in neurofascin (Nfasc) reveals domain-specific requirements in myelinated axons. J Neurosci. 2010;30:4868-76 pubmed publisher
    ..encoding the critical paranodal proteins Caspr, contactin (Cont), and the myelinating glia-specific isoform of Neurofascin (Nfasc(NF155)) results in the disruption of the paranodal axo-glial junctions, loss of ion channel segregation, ..
  2. Scheiermann C, Meda P, Aurrand Lions M, Madani R, Yiangou Y, Coffey P, et al. Expression and function of junctional adhesion molecule-C in myelinated peripheral nerves. Science. 2007;318:1472-5 pubmed
    ..These results demonstrate that JAM-C is a component of the autotypic junctional attachments of Schwann cells and plays an important role in maintaining the integrity and function of myelinated peripheral nerves. ..
  3. Pomicter A, Deloyht J, Hackett A, Purdie N, Sato Bigbee C, Henderson S, et al. Nfasc155H and MAG are specifically susceptible to detergent extraction in the absence of the myelin sphingolipid sulfatide. Neurochem Res. 2013;38:2490-502 pubmed publisher
    ..Thus, our findings present a potential link between sulfatide perturbation and myelin deterioration in multiple sclerosis. ..
  4. Buttermore E, Piochon C, Wallace M, Philpot B, Hansel C, Bhat M. Pinceau organization in the cerebellum requires distinct functions of neurofascin in Purkinje and basket neurons during postnatal development. J Neurosci. 2012;32:4724-42 pubmed publisher
    ..Loss of cytoskeletal adaptor protein Ankyrin G (AnkG) results in mislocalization of the cell adhesion molecule Neurofascin (Nfasc) at the Purkinje AIS and abnormal organization of the pinceau...
  5. Amor V, Zhang C, Vainshtein A, Zhang A, Zollinger D, Eshed Eisenbach Y, et al. The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier. elife. 2017;6: pubmed publisher
    ..Our results reveal that the paranodal junction-dependent mechanism of Na+channel clustering is mediated by the spectrin-based paranodal axonal cytoskeleton. ..
  6. Fuchs P, Zörer M, Reipert S, Rezniczek G, Propst F, Walko G, et al. Targeted inactivation of a developmentally regulated neural plectin isoform (plectin 1c) in mice leads to reduced motor nerve conduction velocity. J Biol Chem. 2009;284:26502-9 pubmed publisher
    ..This is the first report demonstrating an important role of plectin in a major nerve function. ..
  7. Thaxton C, Pillai A, Pribisko A, Dupree J, Bhat M. Nodes of Ranvier act as barriers to restrict invasion of flanking paranodal domains in myelinated axons. Neuron. 2011;69:244-57 pubmed publisher
    ..Here, we report that genetic ablation of the neuron-specific isoform of Neurofascin (Nfasc(NF¹??)) in vivo results in nodal disorganization, including loss of Na(v) channel and ankyrin-G (AnkG) ..
  8. Zhang A, Desmazieres A, Zonta B, Melrose S, Campbell G, Mahad D, et al. Neurofascin 140 is an embryonic neuronal neurofascin isoform that promotes the assembly of the node of Ranvier. J Neurosci. 2015;35:2246-54 pubmed publisher
    ..The Neurofascin (Nfasc) gene has a unique role in node formation because it encodes glial and neuronal isoforms of neurofascin (..
  9. Nodari A, Previtali S, Dati G, Occhi S, Court F, Colombelli C, et al. Alpha6beta4 integrin and dystroglycan cooperate to stabilize the myelin sheath. J Neurosci. 2008;28:6714-9 pubmed publisher
    ..These data indicate that, similar to its role in skin, alpha6beta4 integrin confers stability to myelin in peripheral nerves. ..
  10. Burmeister M, Ren Q, Makris G, Samson D, Bennett V. Genes for the neuronal immunoglobulin domain cell adhesion molecules neurofascin and Nr-CAM map to mouse chromosomes 1 and 12 and homologous human chromosomes. Mamm Genome. 1996;7:558-9 pubmed
  11. Kwon H, Johnson T, Joe M, Abu Asab M, Zhang J, Chan C, et al. Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling. J Biol Chem. 2013;288:26357-71 pubmed publisher
    ..There, myocilin interacts with gliomedin, neurofascin, and NrCAM, which are essential for node formation and function...
  12. Chang K, Zollinger D, Susuki K, Sherman D, Makara M, Brophy P, et al. Glial ankyrins facilitate paranodal axoglial junction assembly. Nat Neurosci. 2014;17:1673-81 pubmed publisher the glial side of paranodal junctions where they interact with the essential glial junctional component neurofascin 155...
  13. Kirschbaum K, Kriebel M, Kranz E, Pötz O, Volkmer H. Analysis of non-canonical fibroblast growth factor receptor 1 (FGFR1) interaction reveals regulatory and activating domains of neurofascin. J Biol Chem. 2009;284:28533-42 pubmed publisher
    ..Here, we show a new link between FGFR1 and the cell adhesion molecule neurofascin, which is important for neurite outgrowth...
  14. Moscoso L, Sanes J. Expression of four immunoglobulin superfamily adhesion molecules (L1, Nr-CAM/Bravo, neurofascin/ABGP, and N-CAM) in the developing mouse spinal cord. J Comp Neurol. 1995;352:321-34 pubmed
    ..a 12-base-pair insert, plus putative murine orthologs of the chicken cell adhesion molecules Nr-CAM/Bravo and neurofascin. All four mRNAs are expressed in NSC-34 cells, but only neurofascin and the insert-minus form of L1 are ..
  15. van Beuningen S, Will L, Harterink M, Chazeau A, van Battum E, Frias C, et al. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron. 2015;88:1208-1226 pubmed publisher
    ..Thus, TRIM46 defines a unique axonal cytoskeletal compartment for regulating microtubule organization during neuronal development. ..
  16. Southwood C, He C, Garbern J, Kamholz J, Arroyo E, Gow A. CNS myelin paranodes require Nkx6-2 homeoprotein transcriptional activity for normal structure. J Neurosci. 2004;24:11215-25 pubmed
    ..encoding a paranodal microtubule destabilizing protein, stathmin 1, and the paranodal cell adhesion molecules neurofascin and contactin...
  17. Freeman S, Desmazières A, Simonnet J, Gatta M, Pfeiffer F, Aigrot M, et al. Acceleration of conduction velocity linked to clustering of nodal components precedes myelination. Proc Natl Acad Sci U S A. 2015;112:E321-8 pubmed publisher
    ..Finally, we provide evidence that prenodal clusters are also detected in vivo before myelination, further strengthening their physiological relevance. ..
  18. Le Bras B, Fréal A, Czarnecki A, Legendre P, Bullier E, Komada M, et al. In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014;219:1433-50 pubmed publisher
    ..Analysis on the global motor neurons population indicated that Neurofascin follows an identical spatio-temporal distribution, whereas sodium channels and ?4-spectrin only appear along ..
  19. Yamazaki K, Oki T, Tanaka I. Cloning of 5'-flanking region and a polymorphic CTT trinucleotide repeat within 5'-untranslated region of mouse R-type calcium channel alpha1-subunit (Cchra1) gene, and its genetic mapping. Gene. 1998;214:199-204 pubmed
    ..Using this polymorphism, the Cchra1 was mapped to the region of chromosome 1 where the synteny to human chromosome 1q was conserved. ..
  20. Edwards R, Herrera Sosa H, Otto J, Bryan J. Cloning and expression of a murine fascin homolog from mouse brain. J Biol Chem. 1995;270:10764-70 pubmed
    ..Electron cryomicroscopy images show that the reconstituted bundles are highly ordered. However, their fine structure differs from that of echinoid fascin-actin bundles. This structural difference can be attributed to fascin. ..
  21. Desmazieres A, Zonta B, Zhang A, Wu L, Sherman D, Brophy P. Differential stability of PNS and CNS nodal complexes when neuronal neurofascin is lost. J Neurosci. 2014;34:5083-8 pubmed publisher
    ..The Neurofascin (Nfasc) gene encodes neuronal Neurofascin 186 (Nfasc186) at the node and glial Neurofascin 155 at the paranode, ..
  22. Pacharra S, Hanisch F, Breloy I. Neurofascin 186 is O-mannosylated within and outside of the mucin domain. J Proteome Res. 2012;11:3955-64 pubmed publisher
    ..Among these neurofascin was chosen for further analysis as a recombinant probe (mucin domain) and as an endogenous protein from mouse ..
  23. Pillai A, Thaxton C, Pribisko A, Cheng J, Dupree J, Bhat M. Spatiotemporal ablation of myelinating glia-specific neurofascin (Nfasc NF155) in mice reveals gradual loss of paranodal axoglial junctions and concomitant disorganization of axonal domains. J Neurosci Res. 2009;87:1773-93 pubmed publisher
    ..These junctions are formed by interactions between a glial isoform of neurofascin (Nfasc(NF155)) and axonal Caspr and Cont...
  24. Hortsch M. Structural and functional evolution of the L1 family: are four adhesion molecules better than one?. Mol Cell Neurosci. 2000;15:1-10 pubmed
  25. Traka M, Goutebroze L, Denisenko N, Bessa M, Nifli A, Havaki S, et al. Association of TAG-1 with Caspr2 is essential for the molecular organization of juxtaparanodal regions of myelinated fibers. J Cell Biol. 2003;162:1161-72 pubmed
    ..This complex is analogous to that described previously at paranodes, suggesting that similar molecules are crucial for different types of axo-glial interactions. ..
  26. Darbelli L, Vogel G, Almazan G, Richard S. Quaking Regulates Neurofascin 155 Expression for Myelin and Axoglial Junction Maintenance. J Neurosci. 2016;36:4106-20 pubmed publisher
    ..Moreover, deterioration of axoglial junctions of the spinal cord was observed and is consistent with a loss of Neurofascin 155 (Nfasc155) isoform that we confirmed as an alternative splice target of the QKI proteins...
  27. Roche S, Sherman D, Dissanayake K, Soucy G, Desmazieres A, Lamont D, et al. Loss of glial neurofascin155 delays developmental synapse elimination at the neuromuscular junction. J Neurosci. 2014;34:12904-18 pubmed publisher
    ..synapse elimination at the mouse NMJ, where loss of a single glial cell protein, the glial isoform of neurofascin (Nfasc155), was sufficient to disrupt postnatal remodeling of synaptic circuitry...
  28. Pomicter A, Shroff S, Fuss B, Sato Bigbee C, Brophy P, Rasband M, et al. Novel forms of neurofascin 155 in the central nervous system: alterations in paranodal disruption models and multiple sclerosis. Brain. 2010;133:389-405 pubmed publisher
    ..comprised of the neuronal heterocomplex of contactin and contactin-associated protein and the myelin protein neurofascin 155...