Gene Symbol: Neu3
Description: neuraminidase 3
Alias: sialidase-3, N-acetyl-alpha-neuraminidase 3, Sialidase 3 (Membrane sialidase) (Ganglioside sialidase) (N-acetyl-alpha-neuraminidase 3), ganglioside sialidase, membrane sialidase
Species: mouse
Products:     Neu3

Top Publications

  1. Hasegawa T, Yamaguchi K, Wada T, Takeda A, Itoyama Y, Miyagi T. Molecular cloning of mouse ganglioside sialidase and its increased expression in Neuro2a cell differentiation. J Biol Chem. 2000;275:8007-15 pubmed
    ..further the roles and regulation mechanisms of the sialidase in neuronal systems, we have cloned mouse ganglioside sialidase cDNA and observed its expression in Neuro2a cell differentiation...
  2. Sasaki A, Hata K, Suzuki S, Sawada M, Wada T, Yamaguchi K, et al. Overexpression of plasma membrane-associated sialidase attenuates insulin signaling in transgenic mice. J Biol Chem. 2003;278:27896-902 pubmed
    ..Here we demonstrate that mice overexpressing the human ortholog (NEU3) develop diabetic phenotype by 18-22 weeks associated with hyperinsulinemia, islet hyperplasia, and increased beta-..
  3. Yamaguchi K, Hata K, Wada T, Moriya S, Miyagi T. Epidermal growth factor-induced mobilization of a ganglioside-specific sialidase (NEU3) to membrane ruffles. Biochem Biophys Res Commun. 2006;346:484-90 pubmed
    Human ganglioside-specific sialidase, NEU3, localized at cell membranes is thought to regulate various biological processes at cell surfaces...
  4. Zanchetti G, Colombi P, Manzoni M, Anastasia L, Caimi L, Borsani G, et al. Sialidase NEU3 is a peripheral membrane protein localized on the cell surface and in endosomal structures. Biochem J. 2007;408:211-9 pubmed
    Sialidase NEU3 is also known as the plasma-membrane-associated form of mammalian sialidases, exhibiting a high substrate specificity towards gangliosides...
  5. Shiozaki K, Koseki K, Yamaguchi K, Shiozaki M, Narimatsu H, Miyagi T. Developmental change of sialidase neu4 expression in murine brain and its involvement in the regulation of neuronal cell differentiation. J Biol Chem. 2009;284:21157-64 pubmed publisher
    ..We previously demonstrated that sialidase Neu3, also rich in mouse brain, is up-regulated during neuronal differentiation with involvement in acceleration of ..
  6. Shiozaki K, Yamaguchi K, Sato I, Miyagi T. Plasma membrane-associated sialidase (NEU3) promotes formation of colonic aberrant crypt foci in azoxymethane-treated transgenic mice. Cancer Sci. 2009;100:588-94 pubmed publisher
    Human plasma membrane-associated sialidase (NEU3) specifically hydrolyzes gangliosides, and it is up-regulated in colon cancer and plays an essential role in the expression of malignant phenotypes...
  7. Papini N, Anastasia L, Tringali C, Croci G, Bresciani R, Yamaguchi K, et al. The plasma membrane-associated sialidase MmNEU3 modifies the ganglioside pattern of adjacent cells supporting its involvement in cell-to-cell interactions. J Biol Chem. 2004;279:16989-95 pubmed
    ..These results indicate that the plasma membrane-associated sialidase MmNEU3 is able to hydrolyze ganglioside substrates in intact living cells at a neutral pH, mainly through cell-to-cell interactions. ..
  8. Valaperta R, Valsecchi M, Rocchetta F, Aureli M, Prioni S, Prinetti A, et al. Induction of axonal differentiation by silencing plasma membrane-associated sialidase Neu3 in neuroblastoma cells. J Neurochem. 2007;100:708-19 pubmed
    A reduction of 70% of the plasma membrane-associated sialidase Neu3 activity, due to a corresponding reduction of the enzyme expression by transducing cells with a short hairpin RNA encoding a sequence target (complementary messenger of ..
  9. Yamaguchi K, Miyagi T. [Function of NEU3 sialidase in membrane microdomains]. Tanpakushitsu Kakusan Koso. 2008;53:1570-4 pubmed

More Information


  1. Moon S, Cho S, Kim K, Jeon J, Ko J, Kim B, et al. Overexpression of membrane sialic acid-specific sialidase Neu3 inhibits matrix metalloproteinase-9 expression in vascular smooth muscle cells. Biochem Biophys Res Commun. 2007;356:542-7 pubmed
    The ganglioside-specific sialidase Neu3 has been suggested to participate in cell growth, migration, and differentiation...
  2. Ma F, Wu D, Deng L, Secrest P, Zhao J, Varki N, et al. Sialidases on mammalian sperm mediate deciduous sialylation during capacitation. J Biol Chem. 2012;287:38073-9 pubmed publisher
    ..We report the presence of two sialidases (neuraminidases Neu1 and Neu3) on mammalian sperm. These are themselves shed from sperm during capacitation...
  3. Scaringi R, Piccoli M, Papini N, Cirillo F, Conforti E, Bergante S, et al. NEU3 sialidase is activated under hypoxia and protects skeletal muscle cells from apoptosis through the activation of the epidermal growth factor receptor signaling pathway and the hypoxia-inducible factor (HIF)-1?. J Biol Chem. 2013;288:3153-62 pubmed publisher
    b>NEU3 sialidase, a key enzyme in ganglioside metabolism, is activated under hypoxic conditions in cultured skeletal muscle cells (C2C12)...
  4. Bonardi D, Papini N, Pasini M, Dileo L, Orizio F, Monti E, et al. Sialidase NEU3 dynamically associates to different membrane domains specifically modifying their ganglioside pattern and triggering Akt phosphorylation. PLoS ONE. 2014;9:e99405 pubmed publisher
    ..Sialidase NEU3 shows high enzymatic specificity toward gangliosides...
  5. Seyrantepe V, Demir S, Timur Z, von Gerichten J, Marsching C, Erdemli E, et al. Murine Sialidase Neu3 facilitates GM2 degradation and bypass in mouse model of Tay-Sachs disease. Exp Neurol. 2018;299:26-41 pubmed publisher
    ..To determine if the sialidase NEU3 contributes to GM2 ganglioside degradation, we generated a mouse model with combined deficiencies of HEXA and NEU3...
  6. Anastasia L, Holguera J, Bianchi A, D Avila F, Papini N, Tringali C, et al. Over-expression of mammalian sialidase NEU3 reduces Newcastle disease virus entry and propagation in COS7 cells. Biochim Biophys Acta. 2008;1780:504-12 pubmed
    ..pattern determined by over-expression of the mammalian plasma-membrane associated, ganglioside specific, sialidase NEU3 would affect the virus-host cell interactions...
  7. Anastasia L, Papini N, Colazzo F, Palazzolo G, Tringali C, Dileo L, et al. NEU3 sialidase strictly modulates GM3 levels in skeletal myoblasts C2C12 thus favoring their differentiation and protecting them from apoptosis. J Biol Chem. 2008;283:36265-71 pubmed publisher
    Membrane-bound sialidase NEU3, often referred to as the "ganglioside sialidase," has a critical regulatory function on the sialoglycosphingolipid pattern of the cell membrane, with an anti-apoptotic function, especially in ..
  8. Paolini L, Orizio F, Busatto S, Radeghieri A, Bresciani R, Bergese P, et al. Exosomes Secreted by HeLa Cells Shuttle on Their Surface the Plasma Membrane-Associated Sialidase NEU3. Biochemistry. 2017;56:6401-6408 pubmed publisher
    ..The plasma membrane-associated sialidase NEU3 is involved in the fine-tuning of sialic acid-containing glycans directly on the cell surface and plays relevant ..
  9. Papini N, Anastasia L, Tringali C, Dileo L, Carubelli I, Sampaolesi M, et al. MmNEU3 sialidase over-expression in C2C12 myoblasts delays differentiation and induces hypertrophic myotube formation. J Cell Biochem. 2012;113:2967-78 pubmed publisher
    ..In order to support the role of the plasma membrane-associated sialidase NEU3 in skeletal muscle differentiation and to analyse which events of the process are mainly affected by this sialidase,..
  10. Pan X, De Aragão C, Velasco Martin J, Priestman D, Wu H, Takahashi K, et al. Neuraminidases 3 and 4 regulate neuronal function by catabolizing brain gangliosides. FASEB J. 2017;31:3467-3483 pubmed publisher
    ..Wu, H. Y., Takahashi, K., Yamaguchi, K., Sturiale, L., Garozzo, D., Platt, F. M., Lamarche-Vane, N., Morales, C. R., Miyagi, T., Pshezhetsky, A. V. Neuraminidases 3 and 4 regulate neuronal function by catabolizing brain gangliosides. ..
  11. Yamaguchi K, Shiozaki K, Moriya S, Koseki K, Wada T, Tateno H, et al. Reduced susceptibility to colitis-associated colon carcinogenesis in mice lacking plasma membrane-associated sialidase. PLoS ONE. 2012;7:e41132 pubmed publisher
    ..We previously reported that the membrane-associated mammalian sialidase NEU3, which preferentially acts on gangliosides, is involved in cell differentiation, motility, and tumorigenesis...
  12. Lipina C, Nardi F, GRACE H, Hundal H. NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids. Cell Signal. 2015;27:1742-50 pubmed publisher
    The plasma membrane-associated enzyme NEU3 sialidase functions to cleave sialic acid residues from the ganglioside GM3 thereby promoting its degradation, and has been implicated in the modulation of insulin action...