Nat1

Summary

Gene Symbol: Nat1
Description: N-acetyl transferase 1
Alias: Nat-1, arylamine N-acetyltransferase 1, N(alpha)-acetyltransferase 15, NatA auxiliary subunit, N-acetyl transferase 1, liver, blood, N-acetyltransferase type 1, arylamide acetylase 1
Species: mouse
Products:     Nat1

Top Publications

  1. Martell K, Levy G, Weber W. Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products. Mol Pharmacol. 1992;42:265-72 pubmed
    ..Comparison of the rates of translation of the rapid and slow isoforms of NAT-2 in an in vitro system showed that NAT-2(99asn) was translated at approximately twice the rate of NAT-2(99ile).(ABSTRACT TRUNCATED AT 400 WORDS) ..
  2. Sugamori K, Brenneman D, Wong S, Gaedigk A, Yu V, Abramovici H, et al. Effect of arylamine acetyltransferase Nat3 gene knockout on N-acetylation in the mouse. Drug Metab Dispos. 2007;35:1064-70 pubmed
    ..Mice possess three NAT isoenzymes: Nat1, Nat2, and Nat3...
  3. Fretland A, Doll M, Gray K, Feng Y, Hein D. Cloning, sequencing, and recombinant expression of NAT1, NAT2, and NAT3 derived from the C3H/HeJ (rapid) and A/HeJ (slow) acetylator inbred mouse: functional characterization of the activation and deactivation of aromatic amine carcinogens. Toxicol Appl Pharmacol. 1997;142:360-6 pubmed
    ..The NAT1, NAT2, and NAT3 genes from C3H/HeJ and A/HeJ acetylator inbred mouse strains were amplified using the polymerase ..
  4. Kelly S, Sim E. Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro. Biochem J. 1994;302 ( Pt 2):347-53 pubmed
    ..The products of all three genes are demonstrated to catalyse acetylation of aminofluorene and anisidine following expression in Escherichia coli. ..
  5. Sugamori K, Wong S, Gaedigk A, Yu V, Abramovici H, Rozmahel R, et al. Generation and functional characterization of arylamine N-acetyltransferase Nat1/Nat2 double-knockout mice. Mol Pharmacol. 2003;64:170-9 pubmed
    ..To facilitate the study of these processes, we have generated a Nat1/Nat2 double-knockout mouse model by gene targeting in embryonic stem cells...
  6. Boukouvala S, Price N, Sim E. Identification and functional characterization of novel polymorphisms associated with the genes for arylamine N-acetyltransferases in mice. Pharmacogenetics. 2002;12:385-94 pubmed
    ..In mice, three NAT isoenzymes are encoded by Nat1, Nat2 and Nat3 genes...
  7. Ohkawa N, Sugisaki S, Tokunaga E, Fujitani K, Hayasaka T, Setou M, et al. N-acetyltransferase ARD1-NAT1 regulates neuronal dendritic development. Genes Cells. 2008;13:1171-83 pubmed publisher
    ARD1 and NAT1 constitute an N-acetyltransferase complex where ARD1 holds the enzymatic activity of the complex. The ARD1-NAT1 complex mediates N-terminal acetylation of nascent polypeptides that emerge from ribosomes after translation...
  8. Glowinski I, Weber W. Genetic regulation of aromatic amine N-acetylation in inbred mice. J Biol Chem. 1982;257:1424-30 pubmed
    ..Analysis of 11 recombinant inbred strains derived from matings of A/J with C57BL/6J mice support this conclusion and demonstrate the existence of minor modifying genes that segregate independently of the major N-acetyltransferase gene. ..
  9. Mitchell M, Futscher B, McQueen C. Developmental expression of N-acetyltransferases in C57BI/6 mice. Drug Metab Dispos. 1999;27:261-4 pubmed
    ..Two genes (NAT1, NAT2) have been identified and allelic variation in NAT2 has been associated with arylamine toxicity in adults...
  10. Laurieri N, Kawamura A, Westwood I, Varney A, Morris E, Russell A, et al. Differences between murine arylamine N-acetyltransferase type 1 and human arylamine N-acetyltransferase type 2 defined by substrate specificity and inhibitor binding. BMC Pharmacol Toxicol. 2014;15:68 pubmed publisher
    The mouse has three arylamine N-acetyltransferase genes, (MOUSE)Nat1, (MOUSE)Nat2 and (MOUSE)Nat3. These are believed to correspond to (HUMAN)NAT1, (HUMAN)NAT2 and NATP in humans...

Detail Information

Publications31

  1. Martell K, Levy G, Weber W. Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products. Mol Pharmacol. 1992;42:265-72 pubmed
    ..Comparison of the rates of translation of the rapid and slow isoforms of NAT-2 in an in vitro system showed that NAT-2(99asn) was translated at approximately twice the rate of NAT-2(99ile).(ABSTRACT TRUNCATED AT 400 WORDS) ..
  2. Sugamori K, Brenneman D, Wong S, Gaedigk A, Yu V, Abramovici H, et al. Effect of arylamine acetyltransferase Nat3 gene knockout on N-acetylation in the mouse. Drug Metab Dispos. 2007;35:1064-70 pubmed
    ..Mice possess three NAT isoenzymes: Nat1, Nat2, and Nat3...
  3. Fretland A, Doll M, Gray K, Feng Y, Hein D. Cloning, sequencing, and recombinant expression of NAT1, NAT2, and NAT3 derived from the C3H/HeJ (rapid) and A/HeJ (slow) acetylator inbred mouse: functional characterization of the activation and deactivation of aromatic amine carcinogens. Toxicol Appl Pharmacol. 1997;142:360-6 pubmed
    ..The NAT1, NAT2, and NAT3 genes from C3H/HeJ and A/HeJ acetylator inbred mouse strains were amplified using the polymerase ..
  4. Kelly S, Sim E. Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro. Biochem J. 1994;302 ( Pt 2):347-53 pubmed
    ..The products of all three genes are demonstrated to catalyse acetylation of aminofluorene and anisidine following expression in Escherichia coli. ..
  5. Sugamori K, Wong S, Gaedigk A, Yu V, Abramovici H, Rozmahel R, et al. Generation and functional characterization of arylamine N-acetyltransferase Nat1/Nat2 double-knockout mice. Mol Pharmacol. 2003;64:170-9 pubmed
    ..To facilitate the study of these processes, we have generated a Nat1/Nat2 double-knockout mouse model by gene targeting in embryonic stem cells...
  6. Boukouvala S, Price N, Sim E. Identification and functional characterization of novel polymorphisms associated with the genes for arylamine N-acetyltransferases in mice. Pharmacogenetics. 2002;12:385-94 pubmed
    ..In mice, three NAT isoenzymes are encoded by Nat1, Nat2 and Nat3 genes...
  7. Ohkawa N, Sugisaki S, Tokunaga E, Fujitani K, Hayasaka T, Setou M, et al. N-acetyltransferase ARD1-NAT1 regulates neuronal dendritic development. Genes Cells. 2008;13:1171-83 pubmed publisher
    ARD1 and NAT1 constitute an N-acetyltransferase complex where ARD1 holds the enzymatic activity of the complex. The ARD1-NAT1 complex mediates N-terminal acetylation of nascent polypeptides that emerge from ribosomes after translation...
  8. Glowinski I, Weber W. Genetic regulation of aromatic amine N-acetylation in inbred mice. J Biol Chem. 1982;257:1424-30 pubmed
    ..Analysis of 11 recombinant inbred strains derived from matings of A/J with C57BL/6J mice support this conclusion and demonstrate the existence of minor modifying genes that segregate independently of the major N-acetyltransferase gene. ..
  9. Mitchell M, Futscher B, McQueen C. Developmental expression of N-acetyltransferases in C57BI/6 mice. Drug Metab Dispos. 1999;27:261-4 pubmed
    ..Two genes (NAT1, NAT2) have been identified and allelic variation in NAT2 has been associated with arylamine toxicity in adults...
  10. Laurieri N, Kawamura A, Westwood I, Varney A, Morris E, Russell A, et al. Differences between murine arylamine N-acetyltransferase type 1 and human arylamine N-acetyltransferase type 2 defined by substrate specificity and inhibitor binding. BMC Pharmacol Toxicol. 2014;15:68 pubmed publisher
    The mouse has three arylamine N-acetyltransferase genes, (MOUSE)Nat1, (MOUSE)Nat2 and (MOUSE)Nat3. These are believed to correspond to (HUMAN)NAT1, (HUMAN)NAT2 and NATP in humans...
  11. Tiang J, Butcher N, Cullinane C, Humbert P, Minchin R. RNAi-mediated knock-down of arylamine N-acetyltransferase-1 expression induces E-cadherin up-regulation and cell-cell contact growth inhibition. PLoS ONE. 2011;6:e17031 pubmed publisher
    Arylamine N-acetyltransferase-1 (NAT1) is an enzyme that catalyzes the biotransformation of arylamine and hydrazine substrates. It also has a role in the catabolism of the folate metabolite p-aminobenzoyl glutamate...
  12. Estrada L, Kanelakis K, Levy G, Weber W. Tissue- and gender-specific expression of N-acetyltransferase 2 (Nat2*) during development of the outbred mouse strain CD-1. Drug Metab Dispos. 2000;28:139-46 pubmed
    ..We propose that the observed increase in Nat2 transcript expression in male mice may be a result of androgen regulation during development. ..
  13. Rodrigues Lima F, Dupret J. In silico sequence analysis of arylamine N-acetyltransferases: evidence for an absence of lateral gene transfer from bacteria to vertebrates and first description of paralogs in bacteria. Biochem Biophys Res Commun. 2002;293:783-92 pubmed
    ..These results provide new insight into the taxonomic distribution and evolutionary history of this class of drug-metabolizing enzymes. ..
  14. Stanley L, Copp A, Pope J, Rolls S, Smelt V, Perry V, et al. Immunochemical detection of arylamine N-acetyltransferase during mouse embryonic development and in adult mouse brain. Teratology. 1998;58:174-82 pubmed
    ..The discovery that human NAT1 acts upon p-aminobenzoylgluatamate (p-ABG) to generate p-acetamidobenzoylglutamate (p-AABG), a major urinary ..
  15. Erickson R, McQueen C, Chau B, Gokhale V, Uchiyama M, Toyoda A, et al. An N-ethyl-N-nitrosourea-induced mutation in N-acetyltransferase 1 in mice. Biochem Biophys Res Commun. 2008;370:285-8 pubmed publisher
    ..There are mouse models for variability of human NAT1; however mice with genetic differences in Nat1 (corresponding to human NAT2), have not been available...
  16. Mattano S, Erickson R, Nesbitt M, Weber W. Linkage of Nat and Es-1 in the mouse and development of strains congenic for N-acetyltransferase. J Hered. 1988;79:430-3 pubmed
    ..B6-Natr strain has the Es-1 genotype of the donor strain. These congenic strains will be important in determining the role of the NAT genotype in susceptibility to arylamine-induced cancer and other disorders. ..
  17. Pandey R, Tokuzawa Y, Yang Z, Hayashi E, Ichisaka T, Kajita S, et al. Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice. Proc Natl Acad Sci U S A. 2013;110:16492-7 pubmed publisher
    ..Cell-culture studies with the insect ortholog of TDRD12 suggest a role for the multidomain protein in mediating complex formation with other participants during secondary piRNA biogenesis. ..
  18. Fakis G, Boukouvala S, Buckle V, Payton M, Denning C, Sim E. Chromosome mapping of the genes for murine arylamine N-acetyltransferases (NATs), enzymes involved in the metabolism of carcinogens: identification of a novel upstream noncoding exon for murine Nat2. Cytogenet Cell Genet. 2000;90:134-8 pubmed
    ..The human NAT loci (NAT1, NAT2, and a pseudogene, NATP) have been mapped to human chromosome 8p22, a region frequently deleted in tumours...
  19. Ebihara S, Marks T, Hudson D, Menaker M. Genetic control of melatonin synthesis in the pineal gland of the mouse. Science. 1986;231:491-3 pubmed
    ..Pineal glands from C57BL/6J mice have neither NAT nor HIOMT activity. These results suggest that the two genes involved in melatonin deficiency are responsible for the absence of normal NAT and HIOMT enzyme activity. ..
  20. Dairou J, Dupret J, Rodrigues Lima F. Impairment of the activity of the xenobiotic-metabolizing enzymes arylamine N-acetyltransferases 1 and 2 (NAT1/NAT2) by peroxynitrite in mouse skeletal muscle cells. FEBS Lett. 2005;579:4719-23 pubmed
    ..We show here that functional NAT1 and NAT2 isoforms are expressed in mouse myotubes and that peroxynitrite may impair their activity in these cells...
  21. Sugiyama H, Takahashi K, Yamamoto T, Iwasaki M, Narita M, Nakamura M, et al. Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells. Proc Natl Acad Sci U S A. 2017;114:340-345 pubmed publisher
    Novel APOBEC1 target 1 (Nat1) (also known as "p97," "Dap5," and "Eif4g2") is a ubiquitously expressed cytoplasmic protein that is homologous to the C-terminal two thirds of eukaryotic translation initiation factor 4G (Eif4g1)...
  22. Sugamori K, Brenneman D, Sanchez O, Doll M, Hein D, Pierce W, et al. Reduced 4-aminobiphenyl-induced liver tumorigenicity but not DNA damage in arylamine N-acetyltransferase null mice. Cancer Lett. 2012;318:206-13 pubmed publisher
    ..tumors, we compared 1-year liver tumor incidence following the postnatal exposure of wild-type and NAT-deficient Nat1/2(-/-) mice to ABP...
  23. Hein D, Grant D, Sim E. Update on consensus arylamine N-acetyltransferase gene nomenclature. Pharmacogenetics. 2000;10:291-2 pubmed
  24. Chennamsetty I, Coronado M, Contrepois K, Keller M, Cárcamo Orive I, Sandin J, et al. Nat1 Deficiency Is Associated with Mitochondrial Dysfunction and Exercise Intolerance in Mice. Cell Rep. 2016;17:527-540 pubmed publisher
    ..Here, we examine the cellular mechanism linking NAT2 to IR and find that Nat1 (mouse ortholog of NAT2) is co-regulated with key mitochondrial genes...
  25. Sugiura N, Adams S, Corriveau R. An evolutionarily conserved N-terminal acetyltransferase complex associated with neuronal development. J Biol Chem. 2003;278:40113-20 pubmed
    ..mNAT1 and mNAT2 are highly homologous to yeast NAT1, a gene known to regulate entry into the G0 phase of the cell cycle...
  26. Knowles J, Xie W, Zhang Z, Chennamsetty I, Chennemsetty I, Assimes T, et al. Identification and validation of N-acetyltransferase 2 as an insulin sensitivity gene. J Clin Invest. 2015;125:1739-51 pubmed publisher
    ..In a murine adipocyte cell line, silencing of NAT2 ortholog Nat1 decreased insulin-mediated glucose uptake, increased basal and isoproterenol-stimulated lipolysis, and decreased ..
  27. Wang P, Shehu A, Lu J, Joshi R, Venkataramanan R, Sugamori K, et al. Deficiency of N-acetyltransferase increases the interactions of isoniazid with endobiotics in mouse liver. Biochem Pharmacol. 2017;145:218-225 pubmed publisher
    ..The current study used Nat1/2(-/-) mice to mimic NAT slow metabolizers and to investigate INH metabolism in the liver...
  28. Bonamassa B, Ma Y, Liu D. Glucocorticoid receptor-mediated transcriptional regulation of N-acetyltransferase 1 gene through distal promoter. AAPS J. 2012;14:581-90 pubmed publisher
    Human arylamine N-acetyltransferase 1, (HUMAN)NAT1, is a phase II xenobiotic-metabolizing enzyme that plays an important role in drug and carcinogen biotransformation and cancer development...
  29. Vatsis K, Weber W, Bell D, Dupret J, Evans D, Grant D, et al. Nomenclature for N-acetyltransferases. Pharmacogenetics. 1995;5:1-17 pubmed
    ..For designation of genotypes at a single NAT* locus, a slash serves to separate the alleles; in phenotype designations, which are not italicized, alleles are separated by a comma. ..
  30. Karolyi J, Erickson R, Liu S, Killewald L. Major effects on teratogen-induced facial clefting in mice determined by a single genetic region. Genetics. 1990;126:201-5 pubmed
    ..However, this chromosomal region does not make the resistant strain susceptible to these two teratogens. ..
  31. Martell K, Vatsis K, Weber W. Molecular genetic basis of rapid and slow acetylation in mice. Mol Pharmacol. 1991;40:218-27 pubmed
    ..Two genomic clones, Nat1 and Nat2, were isolated from a C57BL/6J (B6) mouse (rapid acetylator) genomic library...