Gene Symbol: Nanos2
Description: nanos C2HC-type zinc finger 2
Alias: nos2, nanos homolog 2, NOS-2
Species: mouse
Products:     Nanos2

Top Publications

  1. Guerquin M, Duquenne C, Lahaye J, Tourpin S, Habert R, Livera G. New testicular mechanisms involved in the prevention of fetal meiotic initiation in mice. Dev Biol. 2010;346:320-30 pubmed publisher
  2. Cook M, Munger S, Nadeau J, Capel B. Regulation of male germ cell cycle arrest and differentiation by DND1 is modulated by genetic background. Development. 2011;138:23-32 pubmed publisher
    ..Dnd1(Ter/Ter) germ cells show a strong downregulation of male differentiation genes including Nanos2. In susceptible strains, where teratomas initiate around E15.5-E17...
  3. Tsuda M, Sasaoka Y, Kiso M, Abe K, Haraguchi S, Kobayashi S, et al. Conserved role of nanos proteins in germ cell development. Science. 2003;301:1239-41 pubmed
    ..We report the cloning and the functional analyses of nanos2 and nanos3 in mice. These genes are differentially expressed in mouse PGCs...
  4. Bowles J, Feng C, Spiller C, Davidson T, Jackson A, Koopman P. FGF9 suppresses meiosis and promotes male germ cell fate in mice. Dev Cell. 2010;19:440-9 pubmed publisher
  5. Suzuki A, Igarashi K, Aisaki K, Kanno J, Saga Y. NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. Proc Natl Acad Sci U S A. 2010;107:3594-9 pubmed publisher
    ..We have previously shown that NANOS2 plays an important role in both the maintenance and sexual development of germ cells...
  6. Suzuki A, Saba R, Miyoshi K, Morita Y, Saga Y. Interaction between NANOS2 and the CCR4-NOT deadenylation complex is essential for male germ cell development in mouse. PLoS ONE. 2012;7:e33558 pubmed publisher
    ..CNOT1, a component of the CCR4-NOT deadenylation complex, as a direct factor mediating the interaction with NANOS2. We find that the first 10 amino acids (AAs) of NANOS2 are required for this binding...
  7. Suzuki A, Saga Y. Nanos2 suppresses meiosis and promotes male germ cell differentiation. Genes Dev. 2008;22:430-5 pubmed publisher
    ..We show here that Nanos2 plays critical roles in the differentiation of male germ cells...
  8. Gregoire E, Lavery R, Chassot A, Akiyama H, Treier M, Behringer R, et al. Transient development of ovotestes in XX Sox9 transgenic mice. Dev Biol. 2011;349:65-77 pubmed publisher
    ..Finally, ovarian cells of the XX Wt1:Sox9 ovotestis undergo apoptosis during late embryogenesis leading to complete female-to-male sex reversal of the transgenic mice at birth...
  9. Barrios F, Filipponi D, Pellegrini M, Paronetto M, Di Siena S, Geremia R, et al. Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells. J Cell Sci. 2010;123:871-80 pubmed publisher
    ..Deletion of one of these genes, Nanos2, results in male sterility, owing to loss of germ cells during fetal life...

More Information


  1. Western P, Ralli R, Wakeling S, Lo C, Van Den Bergen J, Miles D, et al. Mitotic arrest in teratoma susceptible fetal male germ cells. PLoS ONE. 2011;6:e20736 pubmed publisher
    ..upregulation of p27(KIP1), p15(INK4B), activation of RB, the expression of male germ cell differentiation markers NANOS2, DNMT3L and MILI and repression of the pluripotency network...
  2. Le Bouffant R, Souquet B, Duval N, Duquenne C, Hervé R, Frydman N, et al. Msx1 and Msx2 promote meiosis initiation. Development. 2011;138:5393-402 pubmed publisher
    ..Collectively, our data demonstrate for the first time that some homeobox genes are required for meiosis initiation in the female germ line. ..
  3. Suzuki H, Sada A, Yoshida S, Saga Y. The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev Biol. 2009;336:222-31 pubmed publisher
    ..The detection of the germ cell-specific proteins Nanos2 and Nanos3 as markers of spermatogonia has enabled the clear dissection of complex populations of these cells as ..
  4. Pui H, Saga Y. Gonocytes-to-spermatogonia transition initiates prior to birth in murine testes and it requires FGF signaling. Mech Dev. 2017;144:125-139 pubmed publisher
    ..Taken together, we defined the precise timing of GST and revealed FGF signaling as a master regulator of GST in the perinatal mouse testes. ..
  5. Suzuki A, Niimi Y, Shinmyozu K, Zhou Z, Kiso M, Saga Y. Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development. EMBO Rep. 2016;17:37-46 pubmed publisher
    ..Mouse NANOS2 interacts directly with the CCR4-NOT (CNOT) deadenylase complex, resulting in the suppression of specific RNAs...
  6. Zhou Z, Shirakawa T, Ohbo K, Sada A, Wu Q, Hasegawa K, et al. RNA Binding Protein Nanos2 Organizes Post-transcriptional Buffering System to Retain Primitive State of Mouse Spermatogonial Stem Cells. Dev Cell. 2015;34:96-107 pubmed publisher
    ..Here, we show that Nanos2, an evolutionarily conserved RNA-binding protein, works with other cellular messenger ribonucleoprotein (mRNP) ..
  7. Saba R, Kato Y, Saga Y. NANOS2 promotes male germ cell development independent of meiosis suppression. Dev Biol. 2014;385:32-40 pubmed publisher
    b>NANOS2 is an RNA-binding protein essential for fetal male germ cell development...
  8. Saba R, Wu Q, Saga Y. CYP26B1 promotes male germ cell differentiation by suppressing STRA8-dependent meiotic and STRA8-independent mitotic pathways. Dev Biol. 2014;389:173-81 pubmed publisher
    ..Concomitantly, we found that the male genetic program represented by the expression of NANOS2 and DNMT3L was totally rescued in about half of dKO germ cells, indicating that meiotic entry causes the ..
  9. Piprek R, Kolasa M, Podkowa D, Kloc M, Kubiak J. Cell adhesion molecules expression pattern indicates that somatic cells arbitrate gonadal sex of differentiating bipotential fetal mouse gonad. Mech Dev. 2017;147:17-27 pubmed publisher
    ..This indicates that differential CAMs expression pattern in the somatic cells but not in the germ line arbitrates structural organization of gonadal anlagen into testis or ovary. ..
  10. Chassot A, Gregoire E, Lavery R, Taketo M, de Rooij D, Adams I, et al. RSPO1/?-catenin signaling pathway regulates oogonia differentiation and entry into meiosis in the mouse fetal ovary. PLoS ONE. 2011;6:e25641 pubmed publisher
    ..Our results demonstrate that RSPO1/?-catenin signaling is involved in meiosis in fetal germ cells and contributes to the cellular decision of germ cells to differentiate into oocyte or sperm...
  11. Saga Y. Function of Nanos2 in the male germ cell lineage in mice. Cell Mol Life Sci. 2010;67:3815-22 pubmed publisher
    ..This includes the maintenance of both the primordial germ cells (PGCs) and germline stem cells. In mice, Nanos2 exhibits a unique feature in which its expression is induced only in the germ cells within the sexually determined ..
  12. Tsuda M, Kiso M, Saga Y. Implication of nanos2-3'UTR in the expression and function of nanos2. Mech Dev. 2006;123:440-9 pubmed
    ..To elucidate the function of the 3'untranslated region (UTR) of the nanos2 gene in mice, we compared the phenotypes of lacZ knock-in mice with or without a native nanos2 3'UTR and found ..
  13. Amadei G, Zander M, Yang G, Dumelie J, Vessey J, Lipshitz H, et al. A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis. J Neurosci. 2015;35:15666-81 pubmed publisher
  14. Gill M, Hu Y, Lin Y, Page D. Licensing of gametogenesis, dependent on RNA binding protein DAZL, as a gateway to sexual differentiation of fetal germ cells. Proc Natl Acad Sci U S A. 2011;108:7443-8 pubmed publisher
    ..In C57BL/6 mice, Dazl is required for licensing. Licensing serves as a gateway from the embryonic processes shared between the sexes--germ cell specification and migration--to the sex-specific pathways of oogenesis and spermatogenesis. ..
  15. Geyer C, Saba R, Kato Y, Anderson A, Chappell V, Saga Y, et al. Rhox13 is translated in premeiotic germ cells in male and female mice and is regulated by NANOS2 in the male. Biol Reprod. 2012;86:127 pubmed publisher
    ..gonads in vivo but occurs precociously in neonatal testes exposed to RA in vitro or in fetal male germ cells when NANOS2 is absent in vivo...
  16. Kato Y, Katsuki T, Kokubo H, Masuda A, Saga Y. Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells. Nat Commun. 2016;7:11272 pubmed publisher
    ..While a mammalian Nanos family protein, NANOS2, is required for sexual differentiation of male (XY) germ cells in mice, the underlying mechanisms and the ..
  17. Miles D, Van Den Bergen J, Wakeling S, Anderson R, Sinclair A, Western P. The proto-oncogene Ret is required for male foetal germ cell survival. Dev Biol. 2012;365:101-9 pubmed publisher
    ..Either way, this study identifies the proto-oncogene RET as a novel component of the foetal male germ cell development pathway. ..
  18. Spiller C, Feng C, Jackson A, Gillis A, Rolland A, Looijenga L, et al. Endogenous Nodal signaling regulates germ cell potency during mammalian testis development. Development. 2012;139:4123-32 pubmed publisher
    ..Thus, Nodal signaling provides a molecular control mechanism that regulates male germ cell potency in normal development and testicular cancer. ..
  19. Wu Q, Kanata K, Saba R, Deng C, Hamada H, Saga Y. Nodal/activin signaling promotes male germ cell fate and suppresses female programming in somatic cells. Development. 2013;140:291-300 pubmed publisher
    ..Furthermore, we prove that nodal/activin-A works directly on male germ cells to induce the male-specific gene Nanos2 independently of FGF9...
  20. Deguchi K, Nagamatsu G, Miyachi H, Kato Y, Morita S, Kimura H, et al. Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male mouse germ cells. Biol Reprod. 2013;88:36 pubmed publisher
    ..Interestingly, GLP is upregulated in embryonic male germ cells deficient for Nanos2, which encodes a germ cell-specific RNA-binding protein...
  21. Sada A, Suzuki A, Suzuki H, Saga Y. The RNA-binding protein NANOS2 is required to maintain murine spermatogonial stem cells. Science. 2009;325:1394-8 pubmed publisher
    ..With the use of transgenic mice, we found that the RNA-binding protein NANOS2 is essential for maintaining spermatogonial stem cells...
  22. Suzuki A, Niimi Y, Saga Y. Interaction of NANOS2 and NANOS3 with different components of the CNOT complex may contribute to the functional differences in mouse male germ cells. Biol Open. 2014;3:1207-16 pubmed publisher
    b>NANOS2 and NANOS3 belong to the Nanos family of proteins that contain a conserved zinc finger domain, which consists of two consecutive CCHC-type zinc finger motifs, and contribute to germ cell development in mice...
  23. Sada A, Hasegawa K, Pin P, Saga Y. NANOS2 acts downstream of glial cell line-derived neurotrophic factor signaling to suppress differentiation of spermatogonial stem cells. Stem Cells. 2012;30:280-91 pubmed publisher
    ..cell line-derived neurotrophic factor (GDNF) signal emanated from Sertoli cells and germ cell-intrinsic factor NANOS2 represent key regulators for the maintenance of spermatogonial stem cells...
  24. Wu Q, Fukuda K, Weinstein M, Graff J, Saga Y. SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice. Development. 2015;142:575-86 pubmed publisher
    ..In conclusion, we propose a model in which p38 and SMAD2 signaling coordinate to determine the sexual fate of XY PGCs. ..
  25. Vanhoutteghem A, Messiaen S, Hervé F, Delhomme B, Moison D, Petit J, et al. The zinc-finger protein basonuclin 2 is required for proper mitotic arrest, prevention of premature meiotic initiation and meiotic progression in mouse male germ cells. Development. 2014;141:4298-310 pubmed publisher
    ..In view of the extreme evolutionary conservation of BNC2, the findings described here are likely to apply to many species. ..
  26. Yu M, Mu H, Niu Z, Chu Z, Zhu H, Hua J. miR-34c enhances mouse spermatogonial stem cells differentiation by targeting Nanos2. J Cell Biochem. 2014;115:232-42 pubmed publisher
    ..We found that a conserved microRNA-34c through its target-Nanos2, regulating SSCs' differentiation in mouse...
  27. Krentz A, Murphy M, Sarver A, Griswold M, Bardwell V, Zarkower D. DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary. Dev Biol. 2011;356:63-70 pubmed publisher
    ..Thus DMRT1 controls Stra8 sex-specifically, activating it in the fetal ovary and repressing it in the adult testis. ..
  28. Suzuki A, Tsuda M, Saga Y. Functional redundancy among Nanos proteins and a distinct role of Nanos2 during male germ cell development. Development. 2007;134:77-83 pubmed
    The mouse Nanos proteins, Nanos2 and Nanos3, are required for germ cell development and share a highly conserved zinc-finger domain. The expression patterns of these factors during development, however, differ from each other...
  29. Vissers Y, Hallemeesch M, Soeters P, Lamers W, Deutz N. NOS2 deficiency increases intestinal metabolism both in nonstimulated and endotoxemic mice. Am J Physiol Gastrointest Liver Physiol. 2004;286:G747-51 pubmed
    ..We therefore investigated the role of inducible NOS (NOS2) on intestinal protein and neuronal NOS (NOS1) and endothelial NOS (NOS3) on amino acid metabolism...
  30. Ohta K, Yamamoto M, Lin Y, Hogg N, Akiyama H, Behringer R, et al. Male differentiation of germ cells induced by embryonic age-specific Sertoli cells in mice. Biol Reprod. 2012;86:112 pubmed publisher
    ..5 dpc PGCs, respectively. After culture, PGCs aggregated with 12.5 dpc Sertoli cells increased Nanos2 and Dnmt3l expression...