Nanog

Summary

Gene Symbol: Nanog
Description: Nanog homeobox
Alias: 2410002E02Rik, ENK, ecat4, homeobox protein NANOG, ES cell-associated protein 4, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, homeobox transcription factor Nanog
Species: mouse
Products:     Nanog

Top Publications

  1. Farthing C, Ficz G, Ng R, Chan C, Andrews S, Dean W, et al. Global mapping of DNA methylation in mouse promoters reveals epigenetic reprogramming of pluripotency genes. PLoS Genet. 2008;4:e1000116 pubmed publisher
    ..Comparisons between pluripotent tissues and pMEFs reveal that a number of pluripotency related genes, including Nanog, Lefty1 and Tdgf1, as well as the nucleosome remodeller Smarcd1, are hypomethylated in stem cells and ..
  2. Theunissen T, van Oosten A, Castelo Branco G, Hall J, Smith A, Silva J. Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions. Curr Biol. 2011;21:65-71 pubmed publisher
    ..Here we show that 2i/LIF treatment in clonal lines of pre-iPS cells results in the activation of endogenous Nanog and rapid downregulation of retroviral Oct4 expression...
  3. Wu Q, Chen X, Zhang J, Loh Y, Low T, Zhang W, et al. Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells. J Biol Chem. 2006;281:24090-4 pubmed
    Embryonic stem (ES) cells are pluripotent cells with self-renewing property. Nanog is a homeobox transcription factor required to maintain ES cells in a non-differentiated state...
  4. Nishioka N, Yamamoto S, Kiyonari H, Sato H, Sawada A, Ota M, et al. Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech Dev. 2008;125:270-83 pubmed
    ..Instead, the ICM specific transcription factors, Oct3/4 and Nanog, were expressed in all the blastomeres...
  5. Festuccia N, Osorno R, Halbritter F, Karwacki Neisius V, Navarro P, Colby D, et al. Esrrb is a direct Nanog target gene that can substitute for Nanog function in pluripotent cells. Cell Stem Cell. 2012;11:477-90 pubmed publisher
    Embryonic stem cell (ESC) self-renewal efficiency is determined by the level of Nanog expression. However, the mechanisms by which Nanog functions remain unclear, and in particular, direct Nanog target genes are uncharacterized...
  6. Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell. 2003;113:643-55 pubmed
    ..We applied expression cloning in mouse ES cells to isolate a self-renewal determinant. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells...
  7. Booth H, Holland P. Eleven daughters of NANOG. Genomics. 2004;84:229-38 pubmed
    b>Nanog is a recently discovered ANTP class homeobox gene. Mouse Nanog is expressed in the inner cell mass and in embryonic stem cells and has roles in self-renewal and maintenance of pluripotency...
  8. Navarro P, Festuccia N, Colby D, Gagliardi A, Mullin N, Zhang W, et al. OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells. EMBO J. 2012;31:4547-62 pubmed publisher
    b>NANOG, OCT4 and SOX2 form the core network of transcription factors supporting embryonic stem (ES) cell self-renewal...
  9. Muñoz Descalzo S, Rué P, Garcia Ojalvo J, Martinez Arias A. Correlations between the levels of Oct4 and Nanog as a signature for naïve pluripotency in mouse embryonic stem cells. Stem Cells. 2012;30:2683-91 pubmed publisher
    ..pluripotent state is traditionally associated with large absolute levels of certain transcription factors such as Nanog and Oct4...

More Information

Publications85

  1. Loh Y, Wu Q, Chew J, Vega V, Zhang W, Chen X, et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet. 2006;38:431-40 pubmed
    Oct4 and Nanog are transcription factors required to maintain the pluripotency and self-renewal of embryonic stem (ES) cells...
  2. Chen X, Fang F, Liou Y, Ng H. Zfp143 regulates Nanog through modulation of Oct4 binding. Stem Cells. 2008;26:2759-67 pubmed publisher
    ..Chromatin immunoprecipitation and electrophoretic mobility shift assays show the direct binding of Zfp143 to the Nanog proximal promoter...
  3. Storm M, Bone H, Beck C, Bourillot P, Schreiber V, Damiano T, et al. Regulation of Nanog expression by phosphoinositide 3-kinase-dependent signaling in murine embryonic stem cells. J Biol Chem. 2007;282:6265-73 pubmed
    ..pharmacological or genetic tools results in decreased expression of RNA for the homeodomain transcription factor Nanog and decreased Nanog protein levels...
  4. Hamazaki T, Kehoe S, Nakano T, Terada N. The Grb2/Mek pathway represses Nanog in murine embryonic stem cells. Mol Cell Biol. 2006;26:7539-49 pubmed
    The homeobox gene Nanog is a key intrinsic determinant of self renewal in embryonic stem (ES) cells, and its repression leads ES cells to selectively differentiate into primitive endoderm...
  5. Shimoda M, Kanai Azuma M, Hara K, Miyazaki S, Kanai Y, Monden M, et al. Sox17 plays a substantial role in late-stage differentiation of the extraembryonic endoderm in vitro. J Cell Sci. 2007;120:3859-69 pubmed
    ..This event was inhibited by the forced expression of Nanog, a negative regulator of differentiation of ESCs into the ExE...
  6. Jiang J, Chan Y, Loh Y, Cai J, Tong G, Lim C, et al. A core Klf circuitry regulates self-renewal of embryonic stem cells. Nat Cell Biol. 2008;10:353-60 pubmed publisher
    ..immunoprecipitation coupled to microarray assay reveals that these Klf proteins share many common targets of Nanog, suggesting a close functional relationship between these factors...
  7. de Vries W, Evsikov A, Brogan L, Anderson C, Graber J, Knowles B, et al. Reprogramming and differentiation in mammals: motifs and mechanisms. Cold Spring Harb Symp Quant Biol. 2008;73:33-8 pubmed publisher
    ..The fact that OCT4, NANOG, and SOX2 form the core components of the pluripotency circuitry would imply that control at the transcriptional ..
  8. Wu Q, Bruce A, Jedrusik A, Ellis P, Andrews R, Langford C, et al. CARM1 is required in embryonic stem cells to maintain pluripotency and resist differentiation. Stem Cells. 2009;27:2637-2645 pubmed publisher
    ..In CARM1 overexpressing ES cells, histone H3 arginine methylation is also at the Nanog promoter to which CARM1 now associates...
  9. Hough S, Clements I, Welch P, Wiederholt K. Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog. Stem Cells. 2006;24:1467-75 pubmed
    ..After transfection of embryonic stem cells with OCT4- or Nanog-targeted RNAi compounds, levels of OCT4 or Nanog transcript and protein were reduced accordingly...
  10. Rodda D, Chew J, Lim L, Loh Y, Wang B, Ng H, et al. Transcriptional regulation of nanog by OCT4 and SOX2. J Biol Chem. 2005;280:24731-7 pubmed
    b>Nanog, Sox2, and Oct4 are transcription factors all essential to maintaining the pluripotent embryonic stem cell phenotype...
  11. Pan G, Li J, Zhou Y, Zheng H, Pei D. A negative feedback loop of transcription factors that controls stem cell pluripotency and self-renewal. FASEB J. 2006;20:1730-2 pubmed
    ..that ES cells maintain their pluripotent state by expressing a battery of transcription factors including Oct4 and Nanog. However, little is known about how ES cells maintain the expression of these pluripotent factors in ES cells...
  12. Aksoy I, Jauch R, Chen J, Dyla M, Divakar U, Bogu G, et al. Oct4 switches partnering from Sox2 to Sox17 to reinterpret the enhancer code and specify endoderm. EMBO J. 2013;32:938-53 pubmed publisher
    ..This work provides insights in understanding cell fate transcriptional regulation by highlighting the direct link between the DNA sequence of an enhancer and a developmental outcome...
  13. Wernig M, Meissner A, Foreman R, Brambrink T, Ku M, Hochedlinger K, et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature. 2007;448:318-24 pubmed
    ..Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells. ..
  14. Hatano S, Tada M, Kimura H, Yamaguchi S, Kono T, Nakano T, et al. Pluripotential competence of cells associated with Nanog activity. Mech Dev. 2005;122:67-79 pubmed publisher
    b>Nanog is a novel pluripotential cell-specific gene that plays a crucial role in maintaining the undifferentiated state of early postimplantation embryos and embryonic stem (ES) cells...
  15. Zhang P, Andrianakos R, Yang Y, Liu C, Lu W. Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression. J Biol Chem. 2010;285:9180-9 pubmed publisher
    ..In addition, Klf4 directly binds to the promoter region of Nanog and regulates its expression...
  16. Niakan K, Ji H, Maehr R, Vokes S, Rodolfa K, Sherwood R, et al. Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal. Genes Dev. 2010;24:312-26 pubmed publisher
    ..cell types and maintain expression of pluripotency-associated transcription factors, including Oct4, Nanog, and Sox2...
  17. Kelly V, Hammer G. LRH-1 and Nanog regulate Dax1 transcription in mouse embryonic stem cells. Mol Cell Endocrinol. 2011;332:116-24 pubmed publisher
    ..Predicated on recent work indicating that Nanog binds to the Dax1 intron, we have used chromatin immunoprecipitation experiments (ChIP) to define an intronic site ..
  18. Nichols J, Smith A. The origin and identity of embryonic stem cells. Development. 2011;138:3-8 pubmed publisher
    ..Based on recent findings, we propose here that ES cells can be derived directly from early epiblast cells and that ES cells might arise via two different routes that are dictated by their culture conditions...
  19. Jedrusik A, Parfitt D, Guo G, Skamagki M, Grabarek J, Johnson M, et al. Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo. Genes Dev. 2008;22:2692-706 pubmed publisher
    ..Thus, divisions generating inside and outside cells are truly asymmetric with respect to cell fate instructions. These two interacting effects ensure the generation of a stable outer epithelium by the blastocyst stage...
  20. Cockburn K, Biechele S, Garner J, Rossant J. The Hippo pathway member Nf2 is required for inner cell mass specification. Curr Biol. 2013;23:1195-201 pubmed publisher
    ..Together, these data establish a clear role for Nf2 upstream of Yap in the preimplantation embryo and demonstrate that Hippo signaling is essential to segregate the ICM from the TE. ..
  21. Nichols J, Silva J, Roode M, Smith A. Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo. Development. 2009;136:3215-22 pubmed publisher
    ..Throughout the ICM, the epiblast-specific marker Nanog is expressed, and in XX embryos epigenetic silencing of the paternal X chromosome is erased...
  22. Po A, Ferretti E, Miele E, De Smaele E, Paganelli A, Canettieri G, et al. Hedgehog controls neural stem cells through p53-independent regulation of Nanog. EMBO J. 2010;29:2646-58 pubmed publisher
    ..The transcription factor Nanog controls stemness acting as a key determinant of both embryonic SC self-renewal and differentiated somatic cells ..
  23. Zhang J, Tomasini A, Mayer A. RBM19 is essential for preimplantation development in the mouse. BMC Dev Biol. 2008;8:115 pubmed publisher
    ..The timing of developmental arrest occurs after expression of the inner cell mass markers OCT3/4 and NANOG, but prior to the specification of trophectoderm as reflected by CDX2 expression...
  24. Hart A, Hartley L, Ibrahim M, Robb L. Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human. Dev Dyn. 2004;230:187-98 pubmed
    The murine Nanog gene, a member of the homeobox family of DNA binding transcription factors, has been shown recently to maintain pluripotency of embryonic stem cells...
  25. Silva J, Chambers I, Pollard S, Smith A. Nanog promotes transfer of pluripotency after cell fusion. Nature. 2006;441:997-1001 pubmed
    ..One candidate is the variant homeodomain protein Nanog, which has the capacity to entrain undifferentiated ES cell propagation...
  26. Machida K, Tsukamoto H, Mkrtchyan H, Duan L, Dynnyk A, Liu H, et al. Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatic oncogenesis involving stem cell marker Nanog. Proc Natl Acad Sci U S A. 2009;106:1548-53 pubmed publisher
    ..We also identify Nanog, the stem/progenitor cell marker, as a novel downstream gene up-regulated by TLR4 activation and the presence of ..
  27. Fidalgo M, Shekar P, Ang Y, Fujiwara Y, Orkin S, Wang J. Zfp281 functions as a transcriptional repressor for pluripotency of mouse embryonic stem cells. Stem Cells. 2011;29:1705-16 pubmed publisher
    ..properties that are controlled by key components of a core regulatory network including Oct4, Sox2, and Nanog. Understanding molecular underpinnings of these properties requires identification and characterization of ..
  28. Krawchuk D, Honma Yamanaka N, Anani S, Yamanaka Y. FGF4 is a limiting factor controlling the proportions of primitive endoderm and epiblast in the ICM of the mouse blastocyst. Dev Biol. 2013;384:65-71 pubmed publisher
    ..We conclude that the amount of FGF4 is limited and regulates PE/EPI proportions in the mouse embryo...
  29. Zhang J, Wang X, Chen B, Suo G, Zhao Y, Duan Z, et al. Expression of Nanog gene promotes NIH3T3 cell proliferation. Biochem Biophys Res Commun. 2005;338:1098-102 pubmed
    ..However, there are numbers of limitations for in vitro cell proliferation. Nanog is an important self-renewal determinant in embryonic stem cells...
  30. van den Berg D, Zhang W, Yates A, Engelen E, Takacs K, Bezstarosti K, et al. Estrogen-related receptor beta interacts with Oct4 to positively regulate Nanog gene expression. Mol Cell Biol. 2008;28:5986-95 pubmed publisher
    Embryonic stem (ES) cell self-renewal is regulated by transcription factors, including Oct4, Sox2, and Nanog. A number of additional transcriptional regulators of ES cell self-renewal have recently been identified, including the orphan ..
  31. Miyanari Y, Torres Padilla M. Control of ground-state pluripotency by allelic regulation of Nanog. Nature. 2012;483:470-3 pubmed publisher
    ..involves both the resetting of epigenetic marks and the activation of pluripotent-cell-specific genes such as Nanog and Oct4 (also known as Pou5f1)...
  32. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature. 2007;448:313-7 pubmed
    ..Here we show that selection for Nanog expression results in germline-competent iPS cells with increased ES-cell-like gene expression and DNA methylation ..
  33. Kalmar T, Lim C, Hayward P, Muñoz Descalzo S, Nichols J, Garcia Ojalvo J, et al. Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells. PLoS Biol. 2009;7:e1000149 pubmed publisher
    ..stem (ES) cells is associated with the activity of a network of transcription factors with Sox2, Oct4, and Nanog at the core...
  34. Yamaguchi S, Kimura H, Tada M, Nakatsuji N, Tada T. Nanog expression in mouse germ cell development. Gene Expr Patterns. 2005;5:639-46 pubmed
    b>Nanog is a newly identified transcriptional factor bearing a homeodomain and expressed in pluripotential cells of preimplantation and early postimplantation embryos, and embryonic stem (ES) and embryonic germ (EG) cells...
  35. Hattori N, Imao Y, Nishino K, Hattori N, Ohgane J, Yagi S, et al. Epigenetic regulation of Nanog gene in embryonic stem and trophoblast stem cells. Genes Cells. 2007;12:387-96 pubmed
    The Nanog and Oct-4 genes are essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos...
  36. Gu P, LeMenuet D, Chung A, Mancini M, Wheeler D, Cooney A. Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Mol Cell Biol. 2005;25:8507-19 pubmed
    ..Transcription factors such as Oct4 and Nanog are required for self-renewal and maintain the undifferentiated state of ES cells...
  37. Chazaud C, Yamanaka Y, Pawson T, Rossant J. Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway. Dev Cell. 2006;10:615-24 pubmed
    ..5 is already heterogeneous. The EPI- and PE-specific transcription factors, Nanog and Gata6, were expressed in the ICM in a random "salt and pepper" pattern, as early as E3...
  38. Krentz A, Murphy M, Zhang T, Sarver A, Jain S, Griswold M, et al. Interaction between DMRT1 function and genetic background modulates signaling and pluripotency to control tumor susceptibility in the fetal germ line. Dev Biol. 2013;377:67-78 pubmed publisher
    ..Given the strong evidence for involvement of DMRT1 in human TGCT, the downstream genes and pathways identified in this study provide potentially useful candidates for roles in the human disease. ..
  39. Ohnishi Y, Huber W, Tsumura A, Kang M, Xenopoulos P, Kurimoto K, et al. Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages. Nat Cell Biol. 2014;16:27-37 pubmed publisher
    ..These data lead us to propose a model where stochastic cell-to-cell expression heterogeneity followed by signal reinforcement underlies ICM lineage segregation by antagonistically separating equivalent cells. ..
  40. Mullin N, Yates A, Rowe A, Nijmeijer B, Colby D, Barlow P, et al. The pluripotency rheostat Nanog functions as a dimer. Biochem J. 2008;411:227-31 pubmed publisher
    The defining activity of the homeodomain protein Nanog is the ability to confer cytokine-independent self-renewal upon ES (embryonic stem) cells in which it is overexpressed...
  41. Wu D, Yao Z. Isolation and characterization of the murine Nanog gene promoter. Cell Res. 2005;15:317-24 pubmed
    b>Nanog protein is expressed in the interior cells of compacted morulae and maintained till epiblasts but downregulated by implantation stage...
  42. Pereira L, Yi F, Merrill B. Repression of Nanog gene transcription by Tcf3 limits embryonic stem cell self-renewal. Mol Cell Biol. 2006;26:7479-91 pubmed
    ..and differentiation, ESC must carefully control the levels of several transcription factors, including Nanog, Sox2, and Oct4...
  43. Ma T, Wang Z, Guo Y, Pei D. The C-terminal pentapeptide of Nanog tryptophan repeat domain interacts with Nac1 and regulates stem cell proliferation but not pluripotency. J Biol Chem. 2009;284:16071-81 pubmed publisher
    Overexpression of Nanog in mouse embryonic stem (ES) cells has been shown to abrogate the requirement of leukemia inhibitory factor for self-renewal in culture. Little is known about the molecular mechanism of Nanog function...
  44. Kang M, Piliszek A, Artus J, Hadjantonakis A. FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse. Development. 2013;140:267-79 pubmed publisher
    ..Fgf4 heterozygous blastocysts exhibited increased numbers of NANOG-positive EPI cells and reduced numbers of GATA6-positive PrE cells, suggesting that FGF signaling is tightly ..
  45. Apostolou E, Ferrari F, Walsh R, Bar Nur O, Stadtfeld M, Cheloufi S, et al. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming. Cell Stem Cell. 2013;12:699-712 pubmed publisher
    ..Here, we identified a genome-wide, pluripotency-specific interaction network around the Nanog promoter by adapting circular chromosome conformation capture sequencing...
  46. Gerbe F, Cox B, Rossant J, Chazaud C. Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst. Dev Biol. 2008;313:594-602 pubmed
    ..Furthermore, the subcellular location of Lrp2, Disabled-2 (Dab2) and Collagen-IV shows that the epithelial structure is acquired in individual cells through successive steps. ..
  47. Wang J, Levasseur D, Orkin S. Requirement of Nanog dimerization for stem cell self-renewal and pluripotency. Proc Natl Acad Sci U S A. 2008;105:6326-31 pubmed publisher
    ..by transcription factors that form a highly interconnected protein interaction network surrounding the homeobox protein Nanog. Enforced expression of Nanog in mouse ES (mES) cells promotes self-renewal and alleviates their ..
  48. Strumpf D, Mao C, Yamanaka Y, Ralston A, Chawengsaksophak K, Beck F, et al. Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development. 2005;132:2093-102 pubmed
    ..to ICM lineage is attributed to the function of the two transcription factors, Oct4 (encoded by Pou5f1) and Nanog. However, a positive regulator of TE cell fate has not been described...
  49. MacArthur B, Sevilla A, Lenz M, Muller F, Schuldt B, Schuppert A, et al. Nanog-dependent feedback loops regulate murine embryonic stem cell heterogeneity. Nat Cell Biol. 2012;14:1139-47 pubmed publisher
    A number of key regulators of mouse embryonic stem (ES) cell identity, including the transcription factor Nanog, show strong expression fluctuations at the single-cell level. The molecular basis for these fluctuations is unknown...
  50. Costa Y, Ding J, Theunissen T, Faiola F, Hore T, Shliaha P, et al. NANOG-dependent function of TET1 and TET2 in establishment of pluripotency. Nature. 2013;495:370-4 pubmed publisher
    ..thought to reside in a core circuitry of master transcription factors including the homeodomain-containing protein NANOG, which has an essential role in establishing ground state pluripotency during somatic cell reprogramming...
  51. Anton R, Kestler H, Kuhl M. Beta-catenin signaling contributes to stemness and regulates early differentiation in murine embryonic stem cells. FEBS Lett. 2007;581:5247-54 pubmed
    ..Oct-3/4, nanog and Wnt11 were able to repress TCF/beta-catenin transcriptional activity...
  52. Messerschmidt D, Kemler R. Nanog is required for primitive endoderm formation through a non-cell autonomous mechanism. Dev Biol. 2010;344:129-37 pubmed publisher
    Early lineage segregation in mouse development results in two, either CDX2- or OCT4/NANOG-positive, cell populations. CDX2-positive cells form the trophectoderm (TE), OCT4/NANOG-positive cells the inner cell mass (ICM)...
  53. Wu G, Han D, Gong Y, Sebastiano V, Gentile L, Singhal N, et al. Establishment of totipotency does not depend on Oct4A. Nat Cell Biol. 2013;15:1089-97 pubmed publisher
    ..After complete inactivation of both maternal and zygotic Oct4A expression, the embryos still formed Oct4-GFP- and Nanog-expressing inner cell masses, albeit non-pluripotent, indicating that Oct4A is not a determinant for the ..
  54. Suzuki A, Raya A, Kawakami Y, Morita M, Matsui T, Nakashima K, et al. Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells. Proc Natl Acad Sci U S A. 2006;103:10294-10299 pubmed publisher
    ..BMP) and leukemia inhibitory factor signaling pathways and the expression of the transcription factors Oct4 and Nanog. However, the network of interactions among extrinsic and intrinsic determinants of ES cell pluripotency is ..
  55. Pan G, Pei D. The stem cell pluripotency factor NANOG activates transcription with two unusually potent subdomains at its C terminus. J Biol Chem. 2005;280:1401-7 pubmed
    ..b>NANOG, an NK-2 type homeodomain gene, has been proposed to play a key role in maintaining stem cell pluripotency ..
  56. Yamaguchi S, Kurimoto K, Yabuta Y, Sasaki H, Nakatsuji N, Saitou M, et al. Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells. Development. 2009;136:4011-20 pubmed publisher
    The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse embryos die soon after implantation...
  57. Gill M, Hu Y, Lin Y, Page D. Licensing of gametogenesis, dependent on RNA binding protein DAZL, as a gateway to sexual differentiation of fetal germ cells. Proc Natl Acad Sci U S A. 2011;108:7443-8 pubmed publisher
    ..In C57BL/6 mice, Dazl is required for licensing. Licensing serves as a gateway from the embryonic processes shared between the sexes--germ cell specification and migration--to the sex-specific pathways of oogenesis and spermatogenesis. ..
  58. Le Bin G, Muñoz Descalzo S, Kurowski A, Leitch H, Lou X, Mansfield W, et al. Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development. 2014;141:1001-10 pubmed publisher
    ..maternal and zygotic Oct4 suggest that it is dispensable for zygote formation, early cleavage and activation of Nanog expression...
  59. Blij S, Frum T, Akyol A, Fearon E, Ralston A. Maternal Cdx2 is dispensable for mouse development. Development. 2012;139:3969-72 pubmed publisher
    ..Here, we genetically ablated maternal Cdx2 using a Cre/lox strategy, and we definitively establish that maternal Cdx2 is not essential for mouse development. ..
  60. Robertson M, Stenhouse F, Colby D, Marland J, Nichols J, Tweedie S, et al. Nanog retrotransposed genes with functionally conserved open reading frames. Mamm Genome. 2006;17:732-43 pubmed
    The Nanog gene plays a key role in the pluripotency of early embryonic cells in vitro and in vivo. In this article retrotransposed copies of Nanog, termed NanogPc and NanogPd, are identified on mouse Chromosomes 4 and 7, respectively...
  61. Lin T, Chao C, Saito S, Mazur S, Murphy M, Appella E, et al. p53 induces differentiation of mouse embryonic stem cells by suppressing Nanog expression. Nat Cell Biol. 2005;7:165-71 pubmed
    ..p53 binds to the promoter of Nanog, a gene required for ESC self-renewal, and suppresses Nanog expression after DNA damage...
  62. Tam W, Lim C, Han J, Zhang J, Ang Y, Ng H, et al. T-cell factor 3 regulates embryonic stem cell pluripotency and self-renewal by the transcriptional control of multiple lineage pathways. Stem Cells. 2008;26:2019-31 pubmed publisher
    ..we find that in mouse preimplantation development embryos, Tcf3 expression is coregulated with Oct4 and Nanog and becomes localized to the inner cell mass of the blastocyst...
  63. Singh A, Hamazaki T, Hankowski K, Terada N. A heterogeneous expression pattern for Nanog in embryonic stem cells. Stem Cells. 2007;25:2534-42 pubmed
    b>Nanog is a critical homeodomain factor responsible for maintaining embryonic stem (ES) cell self-renewal and pluripotency. Of interest, Nanog expression is not homogeneous in the conventional culture of murine ES cells...
  64. Ahmed K, Dehghani H, Rugg Gunn P, Fussner E, Rossant J, Bazett Jones D. Global chromatin architecture reflects pluripotency and lineage commitment in the early mouse embryo. PLoS ONE. 2010;5:e10531 pubmed publisher
    ..Together, these studies show that an open chromatin architecture is established in the embryonic lineages during development and is sufficient to distinguish pluripotent cells from tissue-restricted progenitor cells. ..
  65. Tan M, Au K, Leong D, Foygel K, Wong W, Yao M. An Oct4-Sall4-Nanog network controls developmental progression in the pre-implantation mouse embryo. Mol Syst Biol. 2013;9:632 pubmed publisher
    ..We delineated the regulons of Oct4, Sall4, and Nanog and identified a set of metabolism- and transport-related genes that were controlled by these transcription ..
  66. Frum T, Halbisen M, Wang C, Amiri H, Robson P, Ralston A. Oct4 cell-autonomously promotes primitive endoderm development in the mouse blastocyst. Dev Cell. 2013;25:610-22 pubmed publisher
    ..Finally, we show that Oct4 is required for the expression of multiple EPI and PE genes as well as multiple metabolic pathways essential for the continued growth of the preimplantation embryo. ..
  67. Do D, Ueda J, Messerschmidt D, Lorthongpanich C, Zhou Y, Feng B, et al. A genetic and developmental pathway from STAT3 to the OCT4-NANOG circuit is essential for maintenance of ICM lineages in vivo. Genes Dev. 2013;27:1378-90 pubmed publisher
    Although it is known that OCT4-NANOG are required for maintenance of pluripotent cells in vitro, the upstream signals that regulate this circuit during early development in vivo have not been identified...
  68. Navarro P, Chambers I, Karwacki Neisius V, Chureau C, Morey C, Rougeulle C, et al. Molecular coupling of Xist regulation and pluripotency. Science. 2008;321:1693-5 pubmed publisher
    ..Here we report that key factors supporting pluripotency-Nanog, Oct3/4, and Sox2-bind within Xist intron 1 in undifferentiated embryonic stem (ES) cells...
  69. Plusa B, Piliszek A, Frankenberg S, Artus J, Hadjantonakis A. Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development. 2008;135:3081-91 pubmed publisher
    ..Subsequently, a gradual progression towards a mutually exclusive expression of PrE- and EPI-specific markers occurs. Finally, cell sorting is achieved by a variety of cell behaviours and by selective apoptosis. ..
  70. Maherali N, Sridharan R, Xie W, Utikal J, Eminli S, Arnold K, et al. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell. 2007;1:55-70 pubmed publisher
  71. Schrode N, Saiz N, Di Talia S, Hadjantonakis A. GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst. Dev Cell. 2014;29:454-67 pubmed publisher
    ..differentiate into the pluripotent epiblast or the primitive endoderm (PrE), marked by the transcription factors NANOG and GATA6, respectively...
  72. Ivanova N, Dobrin R, Lu R, Kotenko I, Levorse J, DeCoste C, et al. Dissecting self-renewal in stem cells with RNA interference. Nature. 2006;442:533-8 pubmed
    ..Our studies suggest specific biological roles for these molecules and reveal the complexity of cell fate regulation in embryonic stem cells. ..
  73. Elling U, Klasen C, Eisenberger T, Anlag K, Treier M. Murine inner cell mass-derived lineages depend on Sall4 function. Proc Natl Acad Sci U S A. 2006;103:16319-24 pubmed
    ..These data establish Sall4 as an essential transcription factor required for the early development of inner cell mass-derived cell lineages. ..
  74. Zhang X, Zhang J, Wang T, Esteban M, Pei D. Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells. J Biol Chem. 2008;283:35825-33 pubmed publisher
    ..program of embryonic stem (ES) cells is orchestrated by a core of transcription factors that has OCT4, SOX2, and NANOG as master regulators...
  75. Torres J, Watt F. Nanog maintains pluripotency of mouse embryonic stem cells by inhibiting NFkappaB and cooperating with Stat3. Nat Cell Biol. 2008;10:194-201 pubmed publisher
    ..The transcription factor Nanog is essential in maintaining pluripotency but the mechanisms involved are poorly understood...
  76. Pan G, Thomson J. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007;17:42-9 pubmed
    ..quot; A unique homeobox transcription factor, Nanog, is one of the key downstream effectors of these signals...