Gene Symbol: N WASP
Description: Wiskott-Aldrich syndrome-like (human)
Alias: 2900021I12Rik, 3110031I02Rik, N-WASP, neural Wiskott-Aldrich syndrome protein
Species: mouse
Products:     N WASP

Top Publications

  1. Miki H, Yamaguchi H, Suetsugu S, Takenawa T. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature. 2000;408:732-5 pubmed
    ..From studies of ectopic expression, we found that IRSp53 is essential for Rac to induce membrane ruffling, probably because it recruits WAVE, which stimulates actin polymerization mediated by the Arp2/3 complex. ..
  2. Yamada H, Padilla Parra S, Park S, Itoh T, Chaineau M, Monaldi I, et al. Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. J Biol Chem. 2009;284:34244-56 pubmed publisher
  3. Rivera G, Vasilescu D, Papayannopoulos V, Lim W, Mayer B. A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cells. Mol Cell. 2009;36:525-35 pubmed publisher
    ..This study uncovers a strong reciprocal interdependence between Nck and PI(4,5)P(2) in promoting localized N-WASp-mediated actin polymerization in cells. ..
  4. Gruenbaum Cohen Y, Harel I, Umansky K, Tzahor E, Snapper S, Shilo B, et al. The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc Natl Acad Sci U S A. 2012;109:11211-6 pubmed publisher
    ..WASp-family elements appear therefore to act as universal mediators of the myogenic cell-cell fusion mechanism underlying formation of functional muscle fibers, in both vertebrate and invertebrate species. ..
  5. Lommel S, Benesch S, Rottner K, Franz T, Wehland J, Kuhn R. Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells. EMBO Rep. 2001;2:850-7 pubmed
    ..Our results prove the essential role of this protein for actin cytoskeletal changes induced by these bacterial pathogens in vivo and in addition show for the first time that N-WASP is dispensable for filopodia formation. ..
  6. Rotkopf S, Hamberg Y, Aigaki T, Snapper S, Shilo B, Schejter E. The WASp-based actin polymerization machinery is required in somatic support cells for spermatid maturation and release. Development. 2011;138:2729-39 pubmed publisher
    ..A requirement for WASp-based activity in somatic support cells therefore appears to be a universal feature of spermatogenesis. ..
  7. Westerberg L, Dahlberg C, Baptista M, Moran C, Detre C, Keszei M, et al. Wiskott-Aldrich syndrome protein (WASP) and N-WASP are critical for peripheral B-cell development and function. Blood. 2012;119:3966-74 pubmed publisher
    ..Our results reveal that the combined activity of WASP and N-WASP is required for peripheral B-cell development and function. ..
  8. Salazar M, Kwiatkowski A, Pellegrini L, Cestra G, Butler M, Rossman K, et al. Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl homology domains, links dynamin to regulation of the actin cytoskeleton. J Biol Chem. 2003;278:49031-43 pubmed
    ..The properties of Tuba provide new evidence for a close functional link between dynamin, Rho GTPase signaling, and the actin cytoskeleton. ..
  9. Merrifield C, Qualmann B, Kessels M, Almers W. Neural Wiskott Aldrich Syndrome Protein (N-WASP) and the Arp2/3 complex are recruited to sites of clathrin-mediated endocytosis in cultured fibroblasts. Eur J Cell Biol. 2004;83:13-8 pubmed
    ..We suggest that N-WASP and the Arp2/3 complex trigger actin polymerization during a late step in clathrin-mediated endocytosis, and propel clathrin-coated pits or vesicles from the plasma membrane into the cytoplasm...

More Information


  1. Snapper S, Takeshima F, Anton I, Liu C, Thomas S, Nguyen D, et al. N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility. Nat Cell Biol. 2001;3:897-904 pubmed
    ..These results imply a crucial and non-redundant role for N-WASP in murine embryogenesis and in the actin-based motility of certain pathogens but not in the general formation of actin-containing structures. ..
  2. Park H, Cox D. Cdc42 regulates Fc gamma receptor-mediated phagocytosis through the activation and phosphorylation of Wiskott-Aldrich syndrome protein (WASP) and neural-WASP. Mol Biol Cell. 2009;20:4500-8 pubmed publisher
    ..These results indicate that Cdc42 is essential for the activation of WASP and N-WASP, leading to actin assembly and phagocytic cup formation by macrophages during Fc(gamma)R-mediated phagocytosis. ..
  3. Cotta de Almeida V, Westerberg L, Maillard M, Onaldi D, Wachtel H, Meelu P, et al. Wiskott Aldrich syndrome protein (WASP) and N-WASP are critical for T cell development. Proc Natl Acad Sci U S A. 2007;104:15424-9 pubmed
    ..In summary, this study suggests that, although WASP serves a unique role for peripheral T cell function, T cell development depends on the combined activity of WASP and N-WASP. ..
  4. Kim S, Shilagardi K, Zhang S, Hong S, Sens K, Bo J, et al. A critical function for the actin cytoskeleton in targeted exocytosis of prefusion vesicles during myoblast fusion. Dev Cell. 2007;12:571-86 pubmed
    ..These studies reveal a surprising cell-type specificity of Sltr-mediated actin polymerization in myoblast fusion, and demonstrate that targeted exocytosis of prefusion vesicles is a critical step prior to plasma membrane fusion. ..
  5. Lyubimova A, Garber J, Upadhyay G, Sharov A, Anastasoaie F, Yajnik V, et al. Neural Wiskott-Aldrich syndrome protein modulates Wnt signaling and is required for hair follicle cycling in mice. J Clin Invest. 2010;120:446-56 pubmed publisher
    ..We therefore propose that N-WASP acts as a positive regulator of beta-catenin-dependent transcription, modulating differentiation of HF progenitor cells. ..
  6. Takano K, Watanabe Takano H, Suetsugu S, Kurita S, Tsujita K, Kimura S, et al. Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation. Science. 2010;330:1536-40 pubmed publisher
    ..These findings present the mechanisms of IGF-1-induced actin filament formation in myofibrillogenesis required for muscle maturation and hypertrophy and a mechanism of actin nucleation...
  7. Garber J, Takeshima F, Anton I, Oyoshi M, Lyubimova A, Kapoor A, et al. Enteropathogenic Escherichia coli and vaccinia virus do not require the family of WASP-interacting proteins for pathogen-induced actin assembly. Infect Immun. 2012;80:4071-7 pubmed publisher
    ..Overall, these data highlight similar pathogenic strategies shared by EPEC and vaccinia virus by demonstrating a requirement for both Nck and N-WASP, but not WIP or WIP family members in pathogen-induced actin assembly. ..
  8. Mizutani K, Suetsugu S, Takenawa T. FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation. Biochem Biophys Res Commun. 2004;313:468-74 pubmed
    ..These results indicate that FBP11 regulates localization of N-WASP, thus negatively regulating the function of N-WASP in the cytoplasm. ..
  9. Fukuoka M, Suetsugu S, Miki H, Fukami K, Endo T, Takenawa T. A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42. J Cell Biol. 2001;152:471-82 pubmed
    ..These findings suggest that WISH activates Arp2/3 complex through N-WASP-dependent and -independent pathways without Cdc42, resulting in the rapid actin polymerization required for microspike formation. ..
  10. Lim K, Bu W, Goh W, Koh E, Ong S, Pawson T, et al. The Cdc42 effector IRSp53 generates filopodia by coupling membrane protrusion with actin dynamics. J Biol Chem. 2008;283:20454-72 pubmed publisher
    ..We propose that IRSp53 generates filopodia by coupling membrane protrusion through its I-BAR domain with actin dynamics through SH3 domain binding partners, including N-WASP and Mena. ..
  11. Miller M, Lapetina S, MacGrath S, Sfakianos M, Pollard T, Koleske A. Regulation of actin polymerization and adhesion-dependent cell edge protrusion by the Abl-related gene (Arg) tyrosine kinase and N-WASp. Biochemistry. 2010;49:2227-34 pubmed publisher
    ..These results suggest that Arg promotes actin-based protrusions in response to extracellular stimuli through phosphorylation of and physical interactions with N-WASp. ..
  12. Tanoue T, Takeichi M. Mammalian Fat1 cadherin regulates actin dynamics and cell-cell contact. J Cell Biol. 2004;165:517-28 pubmed
    ..These results suggest that Fat1 regulates actin cytoskeletal organization at cell peripheries, thereby modulating cell contacts and polarity. ..
  13. Zhang X, Moore S, Iskratsch T, Sheetz M. N-WASP-directed actin polymerization activates Cas phosphorylation and lamellipodium spreading. J Cell Sci. 2014;127:1394-405 pubmed publisher
    ..Thus, extension of the leading edge mediated by actin polymerization could stretch Cas during early cell spreading, priming it for phosphorylation. ..
  14. Cai G, Chou C, Hu M, Zheng A, Reichardt L, Guan J, et al. Neuronal Wiskott-Aldrich syndrome protein (N-WASP) is critical for formation of ?-smooth muscle actin filaments during myofibroblast differentiation. Am J Physiol Lung Cell Mol Physiol. 2012;303:L692-702 pubmed publisher
    ..Together, this study supports a critical role of N-WASP in integrating FAK and Arp2/3 signaling to mediate formation of ?-SMA-containing cytoplasmic filaments during myofibroblast differentiation and maturation. ..
  15. Kesavan G, Lieven O, Mamidi A, Ohlin Z, Johansson J, Li W, et al. Cdc42/N-WASP signaling links actin dynamics to pancreatic ? cell delamination and differentiation. Development. 2014;141:685-96 pubmed publisher
    ..These findings elucidate how junctional actin dynamics via Cdc42/N-WASP signaling cell-autonomously control not only epithelial delamination but also cell differentiation during mammalian organogenesis. ..
  16. Isaac B, Ishihara D, Nusblat L, Gevrey J, Dovas A, Condeelis J, et al. N-WASP has the ability to compensate for the loss of WASP in macrophage podosome formation and chemotaxis. Exp Cell Res. 2010;316:3406-16 pubmed publisher
    ..Our study suggests that expression levels of WASP and N-WASP may influence their roles in actin cytoskeleton rearrangement and shed light to the complex intertwining roles WASP and N-WASP play in macrophages. ..
  17. Misra A, Rajmohan R, Lim R, Bhattacharyya S, Thanabalu T. The mammalian verprolin, WIRE induces filopodia independent of N-WASP through IRSp53. Exp Cell Res. 2010;316:2810-24 pubmed publisher
    ..Thus our results show that Cdc42 regulates the activity of IRSp53 by regulating the IRSp53-WIRE interaction as well as localization of the complex to plasma membrane to generate filopodia. ..
  18. Park S, Kim M, Yun S, Ryu J, Park J, Seo B, et al. C(16)-Ceramide-induced F-actin regulation stimulates mouse embryonic stem cell migration: involvement of N-WASP/Cdc42/Arp2/3 complex and cofilin-1/?-actinin. Biochim Biophys Acta. 2013;1831:350-60 pubmed publisher
  19. Novak N, Bar V, Sabanay H, Frechter S, Jaegle M, Snapper S, et al. N-WASP is required for membrane wrapping and myelination by Schwann cells. J Cell Biol. 2011;192:243-50 pubmed publisher
    ..These data suggest that regulation of actin filament nucleation in Schwann cells by N-WASP is crucial for membrane wrapping, longitudinal extension, and myelination. ..
  20. Cheerathodi M, Vincent J, Ballif B. Quantitative comparison of CrkL-SH3 binding proteins from embryonic murine brain and liver: Implications for developmental signaling and the quantification of protein species variants in bottom-up proteomics. J Proteomics. 2015;125:104-11 pubmed publisher
    ..Here we compare the identification and quantification of CrkL-SH3 binding partners between embryonic murine brain and liver. We also uncover and quantify tissue-specific variants in CrkL-SH3 binding proteins. ..
  21. Reginensi A, Scott R, Gregorieff A, Bagherie Lachidan M, Chung C, Lim D, et al. Yap- and Cdc42-dependent nephrogenesis and morphogenesis during mouse kidney development. PLoS Genet. 2013;9:e1003380 pubmed publisher
    ..We propose that Yap responds to Cdc42-dependent signals in nephron progenitor cells to activate a genetic program required to shape the functioning nephron. ..
  22. Suetsugu S, Banzai Y, Banzai Y, Kato M, Fukami K, Kataoka Y, et al. Male-specific sterility caused by the loss of CR16. Genes Cells. 2007;12:721-33 pubmed
    ..The level of N-WASP but not the transcript was decreased in the testes and Sertoli cells of the CR16-knockout mice. Therefore, CR16 and N-WASP are suggested to play important roles in spermatogenesis. ..
  23. Lefever T, Pedersen E, Basse A, Paus R, Quondamatteo F, Stanley A, et al. N-WASP is a novel regulator of hair-follicle cycling that controls antiproliferative TGF{beta} pathways. J Cell Sci. 2010;123:128-40 pubmed publisher
    ..These data identify the actin nucleation regulator N-WASP as a novel element of hair-cycle control that modulates the antiproliferative and pro-apoptotic TGFbeta pathway in keratinocytes in vivo and in vitro. ..
  24. Manchanda N, Lyubimova A, Ho H, James M, Gusella J, Ramesh N, et al. The NF2 tumor suppressor Merlin and the ERM proteins interact with N-WASP and regulate its actin polymerization function. J Biol Chem. 2005;280:12517-22 pubmed
    ..This novel function of merlin and the ERMs illustrates a mechanism by which these proteins directly exert their effects on actin reorganization and also provides new insight into N-WASP regulation. ..
  25. Guenin Macé L, Veyron Churlet R, Thoulouze M, Romet Lemonne G, Hong H, Leadlay P, et al. Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation. J Clin Invest. 2013;123:1501-12 pubmed publisher
    ..Our findings should allow for the rationale design of competitive inhibitors of mycolactone binding to N-WASP, with anti-Buruli ulcer therapeutic potential. ..
  26. Gordon F, Nutt C, Cheunsuchon P, Nakayama Y, Provencher K, Rice K, et al. Increased expression of angiogenic genes in the brains of mouse meg3-null embryos. Endocrinology. 2010;151:2443-52 pubmed publisher
    ..In conclusion, Meg3 may play an important role in control of vascularization in the brain and may function as a tumor suppressor in part by inhibiting angiogenesis. ..
  27. Jin F, Dong B, Georgiou J, Jiang Q, Zhang J, Bharioke A, et al. N-WASp is required for Schwann cell cytoskeletal dynamics, normal myelin gene expression and peripheral nerve myelination. Development. 2011;138:1329-37 pubmed publisher
    ..Our observations demonstrate that N-WASp plays an essential role in Schwann cell maturation and myelin formation. ..
  28. Dart A, Donnelly S, Holden D, Way M, Caron E. Nck and Cdc42 co-operate to recruit N-WASP to promote Fc?R-mediated phagocytosis. J Cell Sci. 2012;125:2825-30 pubmed publisher
    ..Efficient phagocytosis, however, only occurs, if the CRIB domain of N-WASP can also interact with Cdc42. Our observations demonstrate that Nck and Cdc42 collaborate to stimulate N-WASP-dependent Fc?R-mediated phagocytosis. ..
  29. Yao G, Su X, Nguyen V, Roberts K, Li X, Takakura A, et al. Polycystin-1 regulates actin cytoskeleton organization and directional cell migration through a novel PC1-Pacsin 2-N-Wasp complex. Hum Mol Genet. 2014;23:2769-79 pubmed publisher
    ..Disruption of this complex contributes to cyst formation in PKD. ..
  30. Jain N, Lim L, Tan W, George B, Makeyev E, Thanabalu T. Conditional N-WASP knockout in mouse brain implicates actin cytoskeleton regulation in hydrocephalus pathology. Exp Neurol. 2014;254:29-40 pubmed publisher
    ..Taken together, our results suggest that N-WASP plays a critical role in normal brain development and implicate actin cytoskeleton regulation as a vulnerable axis frequently deregulated in hydrocephalus. ..
  31. Yi K, Unruh J, Deng M, Slaughter B, Rubinstein B, Li R. Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes. Nat Cell Biol. 2011;13:1252-8 pubmed publisher
    ..Thus, the asymmetric MII spindle position is dynamically maintained as a result of balanced forces governed by the Arp2/3 complex. ..
  32. Otsuki M, Itoh T, Takenawa T. Neural Wiskott-Aldrich syndrome protein is recruited to rafts and associates with endophilin A in response to epidermal growth factor. J Biol Chem. 2003;278:6461-9 pubmed
    ..These results suggest that, upon EGF stimulation, N-WASP interacts with EA through its proline-rich domain to induce the fission step of clathrin-mediated endocytosis. ..
  33. Yao H, Duan M, Buch S. Cocaine-mediated induction of platelet-derived growth factor: implication for increased vascular permeability. Blood. 2011;117:2538-47 pubmed publisher
    ..Understanding the regulation of PDGF-BB expression may provide insights into the development of potential therapeutic targets for neuroinflammation associated with HIV infection and drug abuse. ..
  34. Modregger J, Ritter B, Witter B, Paulsson M, Plomann M. All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis. J Cell Sci. 2000;113 Pt 24:4511-21 pubmed
    ..These characteristics of the PACSIN protein family suggest a general function in recruitment of the interacting proteins to sites of endocytosis. ..
  35. George B, Jain N, Fen Chong P, Hou Tan J, Thanabalu T. Myogenesis defect due to Toca-1 knockdown can be suppressed by expression of N-WASP. Biochim Biophys Acta. 2014;1843:1930-41 pubmed publisher
    ..This is the first study to identify and characterize the role of Toca-1 in myogenesis. ..
  36. Donnelly S, Weisswange I, Zettl M, Way M. WIP provides an essential link between Nck and N-WASP during Arp2/3-dependent actin polymerization. Curr Biol. 2013;23:999-1006 pubmed publisher
    ..Our observations provide important insights into the hierarchy and connections in one of the major cellular signaling networks stimulating Arp2/3 complex-dependent actin polymerization. ..
  37. Schell C, Baumhakl L, Salou S, Conzelmann A, Meyer C, Helmstädter M, et al. N-wasp is required for stabilization of podocyte foot processes. J Am Soc Nephrol. 2013;24:713-21 pubmed publisher
  38. Mitra P, Thanabalu T. Myogenic differentiation depends on the interplay of Grb2 and N-WASP. Biochim Biophys Acta Mol Cell Res. 2017;1864:487-497 pubmed publisher
    ..Thus our results suggest that Grb2 is a negative regulator of myogenesis and reduces myogenic differentiation by inhibiting actin polymerization/remodeling through its C-terminal SH3 domain. ..
  39. Guilherme A, Soriano N, Furcinitti P, Czech M. Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes. J Biol Chem. 2004;279:40062-75 pubmed
    ..Taken together, these results show that EHD1 and EHBP1, but not EHD2, are required for perinuclear localization of GLUT4 and reveal that loss of EHBP1 disrupts insulin-regulated GLUT4 recycling in cultured adipocytes. ..
  40. Misra A, Lim R, Wu Z, Thanabalu T. N-WASP plays a critical role in fibroblast adhesion and spreading. Biochem Biophys Res Commun. 2007;364:908-12 pubmed
    N-WASP (Neural Wiskott Aldrich Syndrome Protein) regulates actin polymerization by activating the Arp2/3 complex and promotes the formation of actin-rich structures such as filopodia...
  41. King S, Worth D, Scales T, Monypenny J, Jones G, Parsons M. ?1 integrins regulate fibroblast chemotaxis through control of N-WASP stability. EMBO J. 2011;30:1705-18 pubmed publisher
    ..This represents a novel mechanism by which integrins cooperate with growth factor receptors to promote localised signalling and directed cell motility. ..
  42. Martinez Quiles N, Ho H, Kirschner M, Ramesh N, Geha R. Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP. Mol Cell Biol. 2004;24:5269-80 pubmed
  43. Lin J, Liu J, Wang Y, Zhu J, Zhou K, Smith N, et al. Differential regulation of cortactin and N-WASP-mediated actin polymerization by missing in metastasis (MIM) protein. Oncogene. 2005;24:2059-66 pubmed
    ..These data imply that MIM regulates cell motility by modulating different Arp2/3 activators in a distinguished manner. ..
  44. Wagener B, Hu M, Zheng A, Zhao X, Che P, Brandon A, et al. Neuronal Wiskott-Aldrich syndrome protein regulates TGF-?1-mediated lung vascular permeability. FASEB J. 2016;30:2557-69 pubmed publisher
    ..Zhao, X., Che, P., Brandon, A., Anjum, N., Snapper, S., Creighton, J., Guan, J.-L., Han, Q., Cai, G.-Q., Han, X., Pittet, J.-F., Ding, Q. Neuronal Wiskott-Aldrich syndrome protein regulates TGF-?1-mediated lung vascular permeability. ..
  45. Falcone S, Roman W, Hnia K, Gache V, Didier N, Lainé J, et al. N-WASP is required for Amphiphysin-2/BIN1-dependent nuclear positioning and triad organization in skeletal muscle and is involved in the pathophysiology of centronuclear myopathy. EMBO Mol Med. 2014;6:1455-75 pubmed publisher
    ..Our results support a role for N-WASP in amphiphysin-2-dependent nuclear positioning and triad organization and in CNM and myotonic dystrophy pathophysiology. ..
  46. Njoo C, Agarwal N, Lutz B, Kuner R. The Cannabinoid Receptor CB1 Interacts with the WAVE1 Complex and Plays a Role in Actin Dynamics and Structural Plasticity in Neurons. PLoS Biol. 2015;13:e1002286 pubmed publisher
    ..This study reports novel signaling mechanisms for cannabinoidergic modulation of the nervous system and demonstrates a previously unreported role for the WAVE1 complex in therapeutic applications of cannabinoids. ..
  47. Uenishi E, Shibasaki T, Takahashi H, Seki C, Hamaguchi H, Yasuda T, et al. Actin dynamics regulated by the balance of neuronal Wiskott-Aldrich syndrome protein (N-WASP) and cofilin activities determines the biphasic response of glucose-induced insulin secretion. J Biol Chem. 2013;288:25851-64 pubmed publisher
    ..Thus, actin dynamics regulated by the balance of N-WASP and cofilin activities determines the biphasic response of GIIS. ..
  48. Boczkowska M, Rebowski G, Petoukhov M, Hayes D, Svergun D, Dominguez R. X-ray scattering study of activated Arp2/3 complex with bound actin-WCA. Structure. 2008;16:695-704 pubmed publisher
    ..The results support a model of activation that is consistent with most of the biochemical observations. ..
  49. Bhattacharya K, Swaminathan K, Peche V, Clemen C, Knyphausen P, Lammers M, et al. Novel Coronin7 interactions with Cdc42 and N-WASP regulate actin organization and Golgi morphology. Sci Rep. 2016;6:25411 pubmed publisher
    ..We conclude that CRN7 spatiotemporally influences F-actin organization and Golgi integrity in a Cdc42- and N-WASP-dependent manner. ..
  50. Mohammadi S, Isberg R. Cdc42 interacts with the exocyst complex to promote phagocytosis. J Cell Biol. 2013;200:81-93 pubmed publisher
    ..Furthermore, disruption of exocyst function through Exo70 depletion led to a defect in large particle internalization, thereby establishing a functional role for the exocyst complex during phagocytosis. ..
  51. Kanzaki M, Furukawa M, Raab W, Pessin J. Phosphatidylinositol 4,5-bisphosphate regulates adipocyte actin dynamics and GLUT4 vesicle recycling. J Biol Chem. 2004;279:30622-33 pubmed
    ..In adipocytes, altered PI(4,5)P2 metabolism has marked effects on GLUT4 endocytosis and intracellular vesicle trafficking due to the derangement of actin dynamics. ..
  52. Mastrototaro G, Liang X, Li X, Carullo P, Piroddi N, Tesi C, et al. Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers. Cardiovasc Res. 2015;107:216-25 pubmed publisher
    ..These results suggest that the nebulette disease causing mutations have dominant gain-of-function effects. ..
  53. Xiao X, Mruk D, Tang E, Massarwa R, Mok K, Li N, et al. N-wasp is required for structural integrity of the blood-testis barrier. PLoS Genet. 2014;10:e1004447 pubmed publisher
    ..These impairments result in a complete arrest of spermatogenesis, underscoring the critical involvement of the somatic compartment of the seminiferous tubules in germ cell maturation. ..
  54. Kleveta G, Borzęcka K, Zdioruk M, Czerkies M, Kuberczyk H, Sybirna N, et al. LPS induces phosphorylation of actin-regulatory proteins leading to actin reassembly and macrophage motility. J Cell Biochem. 2012;113:80-92 pubmed publisher
    ..Disturbances of actin organization induced by cytochalasin D did not inhibit TNF-? production suggesting that LPS-induced cell motility is not required for TNF-? release. ..
  55. You J, Lin Chao S. Gas7 functions with N-WASP to regulate the neurite outgrowth of hippocampal neurons. J Biol Chem. 2010;285:11652-66 pubmed publisher
    ..The data demonstrate an essential role of Gas7 through its interaction with N-WASP during neuronal maturation/differentiation. ..
  56. Franco Villanueva A, Fernández López E, Gabandé Rodríguez E, Bañón Rodríguez I, Esteban J, Anton I, et al. WIP modulates dendritic spine actin cytoskeleton by transcriptional control of lipid metabolic enzymes. Hum Mol Genet. 2014;23:4383-95 pubmed publisher
    ..They also contribute to explain cognitive deficits shared by individuals bearing mutations in the region assigned to the gene encoding for WIP. ..
  57. Kowalski J, Egile C, Gil S, Snapper S, Li R, Thomas S. Cortactin regulates cell migration through activation of N-WASP. J Cell Sci. 2005;118:79-87 pubmed
    ..vitro, cortactin binds to and activates, via its SH3 domain, a regulator of the Arp2/3 complex, neural Wiskott Aldrich Syndrome protein (N-WASP)...
  58. Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, et al. Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol. 2005;168:441-52 pubmed
    ..These results indicate that EGF receptor signaling regulates invadopodium formation through the N-WASP-Arp2/3 pathway and cofilin is necessary for the stabilization and maturation of invadopodia. ..
  59. Legg J, Bompard G, Dawson J, Morris H, Andrew N, Cooper L, et al. N-WASP involvement in dorsal ruffle formation in mouse embryonic fibroblasts. Mol Biol Cell. 2007;18:678-87 pubmed
    ..We hypothesize that N-WASP and Arp2/3 complex are part of a multiprotein assembly important for the generation of dorsal ruffles and that Scar1 and Scar2 are dispensable for this process. ..
  60. Suetsugu S, Takenawa T. Translocation of N-WASP by nuclear localization and export signals into the nucleus modulates expression of HSP90. J Biol Chem. 2003;278:42515-23 pubmed
    ..Because HSP90 is essential for the activities of Src family kinases, these results suggest that localization of N-WASP modulates Src kinase activity by regulating HSP90 expression. ..
  61. Fukumi Tominaga T, Mori Y, Matsuura A, Kaneko K, Matsui M, Ogata M, et al. DIP/WISH-deficient mice reveal Dia- and N-WASP-interacting protein as a regulator of cytoskeletal dynamics in embryonic fibroblasts. Genes Cells. 2009;14:1197-207 pubmed publisher
    ..Thus, DIP/WISH is a negative regulator of Rho and modulates cell adhesion by controlling the integration of adhesion molecules. ..
  62. Liu C, Bai X, Wu J, Sharma S, Upadhyaya A, Dahlberg C, et al. N-wasp is essential for the negative regulation of B cell receptor signaling. PLoS Biol. 2013;11:e1001704 pubmed publisher
    ..Our results reveal a new mechanism for the negative regulation of BCR signaling and broadly suggest an actin-mediated mechanism for signaling down-regulation. ..
  63. Kim H, DiBernardo A, Sloane J, Rasband M, Solomon D, Kosaras B, et al. WAVE1 is required for oligodendrocyte morphogenesis and normal CNS myelination. J Neurosci. 2006;26:5849-59 pubmed
    ..Together, these data demonstrate a role for WAVE1 in oligodendrocyte morphogenesis and myelination. ..
  64. Jaudon F, Raynaud F, Wehrlé R, Bellanger J, Doulazmi M, Vodjdani G, et al. The RhoGEF DOCK10 is essential for dendritic spine morphogenesis. Mol Biol Cell. 2015;26:2112-27 pubmed publisher
    ..Our global approach thus identifies an unprecedented function for DOCK10 as a novel regulator of dendritic spine morphogenesis via a Cdc42-mediated pathway. ..