Mup1

..These findings are consistent with the notion that MUP expression is controlled by a variety of independently assorting genes...

..6 (+/- 3.6)--Ifa--7.9 (+/- 2.8)--m and from a second three-point cross Mup-1--1.7 (+/- 1.7)--b--3.5 (+/- 2.4)--Ifa. The combined results indicate that the gene order, from the centromere, is Mup-1--b--Ifa--m...

..Homologies between different cloned sequences extend over as much as 15 kb. No clone contains parts of two MUP genes, and no homology has been detected between the 3' flanking region of one MUP gene and the 5' flanking region of another...

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..6 centimorgans. None of the lines was either resistant or susceptible to both N- and B-tropic viruses. Nineteen other inbred strains, previously untested, were characterized as either Fv-1n or Fv-1b...

..We also show that some MUP genes may be linked in a tail-to-tail fashion with 26-28 kb between the 3' ends of two genes. This suggests that the minimum distance between successive 45-kb units is approximately 7 kb...

..The 12 acceptor splice sites of the two genes all contain the identical sequence ACAG at the exon boundary.(ABSTRACT TRUNCATED AT 250 WORDS)..

..We show here that mouse urine does indeed contain a glycosylated MUP protein with those properties, presumably the product of the gene that corresponds to pMUP15...

The major urinary proteins (MUPs) of the mouse are encoded by a multigene family located at the Mup a locus on chromosome 4...

..Shahan, M. Denaro, M. Gilmartin, Y. Shi, and E. Derman, Mol. Cell. Biol. 7:1947-1954, 1987), to characterize the expression of MUP genes I through V...

..When a particular MUP gene is expressed in several tissues, transcripts of this gene initiate at the same site and are spliced and polyadenylated in the same manner...

..Both sequences appear to be regulated by growth hormone and testosterone. Southern blot analysis of mouse genomic DNA indicates that there are multiple genes homologous to each sequence...

..An analysis of mouse-Chinese hamster hybrid cell lines shows that most, if not all, Group 1 and Group 2 genes are located on mouse chromosome 4...

..In the two strains there are at least 13 different MUPs, either observed or predicted, indicating the heterogeneity of expression of this group of proteins...

..4 A resolution) and rat urinary alpha 2-globulin (at 2.8 A resolution). The results corroborate the role of these proteins in pheromone transport and elaborate the structural basis of ligand binding...

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..Our results substantiate the idea of MUP proteins as pheromone ligands that mediate male-male aggression through the accessory olfactory neural pathway...

..The shape of the beta-barrel resembles a calyx, lined inside by mostly hydrophobic residues that are instrumental for the binding and transport of small nonpolar ligand molecules...

..Thus, it is concluded that the locus is identical to Mup-1 and it is proposed that Mup-1c be designated as the allele that determines a third phenotypic form of MUPs in MOA mice...

..In addition, the present methods revealed that both liver and serum contained MUP's consisting of components similar to those found in the urine from the same sex and strain...

..Our findings illustrate that increasing genomic complexity of the Mup gene family is not evolutionarily isolated, but is instead a recurring mechanism of generating coding diversity consistent with a species-specific function in mammals...

Major urinary protein (MUP) 1 is a lipocalin family member abundantly secreted into the circulation by the liver. MUP1 binds to lipophilic pheromones and is excreted in urine...

..Silent sites have diverged four times more rapidly than replacement sites, showing that there has been selection against change in the protein sequence...

..The conserved linkage of Aco-1 and Galt in mouse and man probably represents an ancient linkage not yet disrupted by chromosomal rearrangement, rather than a linkage preserved by natural selection...

..The data underline a direct role for Aire in TRA expression and suggest that modulation of Aire has a potential to control central tolerance and autoimmunity...

..The combination of these mapping techniques should be applicable to many cloned DNA sequences...

..Probabilities of linkage were not sufficient for declaration of a quantitative trait locus for REMS but were sufficient to warrant further analysis either with additional RI strains or with F2 panels...

..The chromosomal location was also confirmed by fluorescence in situ hybridization...

..We therefore predict that a human homologue of the wi gene, involved in autosomal recessive deafness, lies in this region of conserved homology on 9q32-q34...

..These results suggest that the Hes-3 and Hes-5 genes may be clustered on chromosome 4 while the Hes-1 gene is not...

..Together, results indicate that a QTL on mouse chromosome 4 explains a significant portion of the genetic variance in O(3)-induced hyperpermeability, and support a role for Tlr4 as a strong candidate susceptibility gene...

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Linkages among three biochemical loci (Aco1, Ahd2, and Mup1) and four microsatellite loci (A8, Glut1, Jun, and Pnd) were determined to construct a linkage map of rat Chromosome (Chr) 5...

..Strain distribution studies show Ly-22.2 is present in all strains examined except B10-derived congenic strains. It is of interest that C57BL/6 and C57BL/10 mice differ in the expression of this antigen...

..6 +/- 4.9 recombination units. The linkage data obtained in the present study place Mos in a region compatible with the physical map (D. W. Threadgill and J. E. Womack, 1988, Genomics 3: 82-86)...

..The phylogenetic grouping correlates with grouping according to aspects of function. The genomes of the inbred strains BALB/c and C57BL contain different MUP gene arrays that we take to be samples from the wild population of arrays...

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..Mtv-30 was genetically mapped to chromosome 12. Additionally, two previously identified Mtv loci, Mtv-14 and Mtv-23, were genetically mapped to chromosome 4 and chromosome 6, respectively...

..2.4.1.38; Ggtb) was localized to the centromeric region of mouse Chromosome 4, between Mtv-14 and Mtv-17, using Southern blot analysis of an intersubspecies backcross...

..5b gene. This finding suggests that the inactivity of the Mup-1.5b gene is brought about by long range-acting mechanisms that establish a chromatin structure in the vicinity of this gene incompatible with transcription...

..Eight (62%) of the 13 repeats that have been mapped lie on Chromosomes (Chr) 4 and 6. This approach is an effective way of generating informative markers on specific chromosomes...

..These findings emphasize the possibility and the necessity of monitoring the genetic purity of inbred strains of mice. A routine monitoring scheme using body protein-polymorphisms is proposed...

..In contrast, at the Map-1 locus, the more negatively charged phenotype is dominant. The electrophoretic pattern of development of the liver enzyme from SM/JCv mice is described...

..The Mup phenotypes described, can be useful for the detection of certain strain contaminations, especially if F1 hybrid Mup phenotypes are distinguishable...

..This linkage map will provide a framework for identifying homologous genes in mice and humans that may be involved in various disease processes...

..Interestingly, the hypergammaglobulinemia and anti-DNA antibody levels in 6-month-old Lpsd mice did not differ from those of NZB mice despite the absence of LPS-responsive CFU-B...

..Analysis of urinary proteins from hybrids between C57BL/6 and M. spretus suggested that low MUP expression in M. spretus females was due to cis-acting genetic elements...

..These linkage studies provide a means for analyzing the genetic control of many of the diverse reactions of the endotoxic response to LPS...

..The effect of Bmfr-2 on B cell responsiveness may be related to levels of the steroid sex hormones. DBA/2Ha mice offer a tool for studying the genetic and hormonal regulation of the immune system...

..R. Callahan, D. Gallahan, and Ch. Kozak (1984), J. Virol. 49, 1005-1008). GR and GR.Mtv-2 furthermore contain two incomplete MMTV proviral elements, one of which is also present in STS/A...

..These findings suggest that a sequence of gene controls characterizes expression of specific genes in developing liver, and that differentiating fetal hepatoblasts are capable of undergoing similar patterns of gene activity in culture...

..This method, extensive to other ligand-modulated transcription factors, might be useful to all transcription factors with slight modifications...

..The microsatellites were distributed to most chromosomes except for chromosomes 16 and 19. These novel markers with defined locations are useful in linkage and genome mapping studies...

..It can therefore be concluded that the IFN-alpha gene cluster is situated on chromosome 4 near the H-15 locus, between loci Mup-1 and b...

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..The principal driving force governing ligand affinity and specificity can be attributed to solvent-driven enthalpic effects from desolvation of the protein binding pocket...

..A human homolog would be expected to be located on human chromosomes 1p or 8q...

..In the intraspecific backcross, wi was found to be non-recombinant with Orm1 and D4Mit244, 0.3 cM distal of Mup1, and 0.6 cM proximal of Tnc...

..This space will not be adequate to support our anticipated continued growth in the areas of infectious disease and toxic challenge and in the need for quarantine of non-vendor rodents. ..

..The data should reveal new and practical strategies for relief of post-procedural pain in mice. ..

..abstract_text> ..

..Achieving this ambitious goal is feasible given the experience of my laboratory in surveying sleep phenotypes in other studies involving large numbers of mice. ..

..Such a long-term result would improve the quality of life and economic welfare of target populations that span many disease conditions. ..

..Such a long-term result would improve the quality of life and economic welfare of target populations that span many disease conditions. ..

..Studying the mechanisms by which genes and their products modulate sleep will ultimately improve our understanding of processes that control normal sleep and contribute to sleep disorders. ..

..Such a long-term result would improve the quality of life and economic welfare of target populations that span many disease conditions. ..