Msra

Summary

Gene Symbol: Msra
Description: methionine sulfoxide reductase A
Alias: 2310045J23Rik, 6530413P12Rik, MSR-A, mitochondrial peptide methionine sulfoxide reductase, PMSR, peptide Met(O) reductase, peptide methionine sulfoxide reductase, peptide-methionine (S)-S-oxide reductase, protein-methionine-S-oxide reductase
Species: mouse
Products:     Msra

Top Publications

  1. Kim H, Gladyshev V. Role of structural and functional elements of mouse methionine-S-sulfoxide reductase in its subcellular distribution. Biochemistry. 2005;44:8059-67 pubmed
    Oxidized forms of methionine residues in proteins can be repaired by methionine-S-sulfoxide reductase (MsrA) and methionine-R-sulfoxide reductase (MsrB)...
  2. Oien D, Osterhaus G, Latif S, Pinkston J, Fulks J, Johnson M, et al. MsrA knockout mouse exhibits abnormal behavior and brain dopamine levels. Free Radic Biol Med. 2008;45:193-200 pubmed publisher
    ..levels of brain pathologies and an abnormal walking pattern in the methionine sulfoxide reductase A knockout (MsrA(-/-)) mouse...
  3. Moskovitz J, Jenkins N, Gilbert D, Copeland N, Jursky F, Weissbach H, et al. Chromosomal localization of the mammalian peptide-methionine sulfoxide reductase gene and its differential expression in various tissues. Proc Natl Acad Sci U S A. 1996;93:3205-8 pubmed
    b>Peptide methionine sulfoxide reductase (MsrA; EC 1.8.4.6) is a ubiquitous protein that can reduce methionine sulfoxide residues in proteins as well as in a large number of methyl sulfoxide compounds...
  4. Moskovitz J, Bar Noy S, Williams W, Requena J, Berlett B, Stadtman E. Methionine sulfoxide reductase (MsrA) is a regulator of antioxidant defense and lifespan in mammals. Proc Natl Acad Sci U S A. 2001;98:12920-5 pubmed
    ..sulfoxide derivatives, these oxidations are readily repaired by the action of methionine sulfoxide reductase (MsrA)...
  5. Pal R, Oien D, Ersen F, Moskovitz J. Elevated levels of brain-pathologies associated with neurodegenerative diseases in the methionine sulfoxide reductase A knockout mouse. Exp Brain Res. 2007;180:765-74 pubmed
    ..In this study, a knockout mouse strain of the methionine sulfoxide reductase A gene (MsrA ( -/- )) caused an enhanced neurodegeneration in brain hippocampus relative to its wild-type control mouse brain...
  6. Kim J, Choi S, Jung K, Lee E, Kim H, Park K. Protective role of methionine sulfoxide reductase A against ischemia/reperfusion injury in mouse kidney and its involvement in the regulation of trans-sulfuration pathway. Antioxid Redox Signal. 2013;18:2241-50 pubmed publisher
    Methionine sulfoxide reductase A (MsrA) and methionine metabolism are associated with oxidative stress, a principal cause of ischemia/reperfusion (I/R) injury...
  7. Salmon A, Perez V, Bokov A, Jernigan A, Kim G, Zhao H, et al. Lack of methionine sulfoxide reductase A in mice increases sensitivity to oxidative stress but does not diminish life span. FASEB J. 2009;23:3601-8 pubmed publisher
    Methionine sulfoxide reductase A (MsrA) repairs oxidized methionine residues within proteins and may also function as a general antioxidant...
  8. Oien D, Moskovitz J. Ablation of the mammalian methionine sulfoxide reductase A affects the expression level of cysteine deoxygenase. Biochem Biophys Res Commun. 2007;352:556-9 pubmed
    ..Lack of methionine sulfoxide reductase A (MsrA) in liver of MsrA-/- led to a significant drop in the cellular level of thiol groups and lowered the CDO level of ..
  9. Erickson J, Joiner M, Guan X, Kutschke W, Yang J, Oddis C, et al. A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation. Cell. 2008;133:462-74 pubmed publisher
    ..CaMKII oxidation is reversed by methionine sulfoxide reductase A (MsrA), and MsrA-/- mice show exaggerated CaMKII oxidation and myocardial apoptosis, impaired cardiac function, and ..

More Information

Publications51

  1. Singh M, Kim K, Kim H. Methionine sulfoxide reductase A deficiency exacerbates acute liver injury induced by acetaminophen. Biochem Biophys Res Commun. 2017;484:189-194 pubmed publisher
    ..Methionine sulfoxide reductase A (MsrA) acts as a reactive oxygen species scavenger by catalyzing the cyclic reduction of methionine-S-sulfoxide...
  2. Brennan L, Lee W, Cowell T, Giblin F, Kantorow M. Deletion of mouse MsrA results in HBO-induced cataract: MsrA repairs mitochondrial cytochrome c. Mol Vis. 2009;15:985-99 pubmed
    Considerable evidence indicates a role for methionine sulfoxide reductase A (MsrA) in lens cell resistance to oxidative stress through its maintenance of mitochondrial function...
  3. Choi S, Kim H. Methionine sulfoxide reductase A regulates cell growth through the p53-p21 pathway. Biochem Biophys Res Commun. 2011;416:70-5 pubmed publisher
    b>MsrA is an oxidoreductase that catalyzes the stereospecific reduction of methionine-S-sulfoxide to methionine...
  4. He B, Joiner M, Singh M, Luczak E, Swaminathan P, Koval O, et al. Oxidation of CaMKII determines the cardiotoxic effects of aldosterone. Nat Med. 2011;17:1610-8 pubmed publisher
  5. Moskovitz J, Maiti P, Lopes D, Oien D, ATTAR A, Liu T, et al. Induction of methionine-sulfoxide reductases protects neurons from amyloid ?-protein insults in vitro and in vivo. Biochemistry. 2011;50:10687-97 pubmed publisher
    ..In agreement with these observations, in MsrA(-/-) mice the difference in toxicity between native A? and A?-sulfoxide was essentially eliminated...
  6. Novoselov S, Kim H, Hua D, Lee B, Astle C, Harrison D, et al. Regulation of selenoproteins and methionine sulfoxide reductases A and B1 by age, calorie restriction, and dietary selenium in mice. Antioxid Redox Signal. 2010;12:829-38 pubmed publisher
    Methionine residues are susceptible to oxidation, but this damage may be reversed by methionine sulfoxide reductases MsrA and MsrB. Mammals contain one MsrA and three MsrBs, including a selenoprotein MsrB1...
  7. Sanders P, Koval O, Jaffer O, Prasad A, Businga T, Scott J, et al. CaMKII is essential for the proasthmatic effects of oxidation. Sci Transl Med. 2013;5:195ra97 pubmed publisher
    ..Our findings support the view that CaMKII is a ROS-responsive, pluripotent proasthmatic signal and provide proof-of-concept evidence that CaMKII is a therapeutic target in asthma. ..
  8. Moskovitz J, Stadtman E. Selenium-deficient diet enhances protein oxidation and affects methionine sulfoxide reductase (MsrB) protein level in certain mouse tissues. Proc Natl Acad Sci U S A. 2003;100:7486-90 pubmed
    Mammals contain two methionine sulfoxide (MetO) reductases, MsrA and MsrB, that catalyze the thioredoxin-dependent reduction of the S-MetO and R-MetO derivatives, respectively, to methionine...
  9. Oien D, Osterhaus G, Lundquist B, Fowler S, Moskovitz J. Caloric restriction alleviates abnormal locomotor activity and dopamine levels in the brain of the methionine sulfoxide reductase A knockout mouse. Neurosci Lett. 2010;468:38-41 pubmed publisher
    ..Previously, we have shown that MsrA(-/-) mice exhibit altered locomotor activity and brain dopamine levels as function of age...
  10. Moskovitz J, Walss Bass C, Cruz D, Thompson P, Bortolato M. Methionine sulfoxide reductase regulates brain catechol-O-methyl transferase activity. Int J Neuropsychopharmacol. 2014;17:1707-13 pubmed publisher
    ..We used recombinant COMT proteins (Val/Met108) and mice (wild-type (WT) and MsrA knockout) to determine the effect of methionine oxidation on COMT activity and COMT interaction with Msr, through a ..
  11. Kim H. Glutaredoxin serves as a reductant for methionine sulfoxide reductases with or without resolving cysteine. Acta Biochim Biophys Sin (Shanghai). 2012;44:623-7 pubmed publisher
    Methionine sulfoxide reductases A and B (MsrA and MsrB) have been known to be thioredoxin (Trx)-dependent enzymes that catalyze the reduction of methionine sulfoxide in a stereospecific manner...
  12. Lee B, Le D, Gladyshev V. Mammals reduce methionine-S-sulfoxide with MsrA and are unable to reduce methionine-R-sulfoxide, and this function can be restored with a yeast reductase. J Biol Chem. 2008;283:28361-9 pubmed publisher
    ..grew on methionine-S-sulfoxide and consumed this compound by import and methionine-S-sulfoxide reductase (MsrA)-dependent reduction, but methionine-R-sulfoxide reductases were not involved in this process, and methionine-R-..
  13. Klutho P, Pennington S, Scott J, Wilson K, Gu S, Doddapattar P, et al. Deletion of Methionine Sulfoxide Reductase A Does Not Affect Atherothrombosis but Promotes Neointimal Hyperplasia and Extracellular Signal-Regulated Kinase 1/2 Signaling. Arterioscler Thromb Vasc Biol. 2015;35:2594-604 pubmed publisher
    ..The objective of this study was to define the role of the methionine sulfoxide reductase A (MsrA) in models of vascular disease and identify its signaling pathways...
  14. Noh M, Kim K, Han S, Kim J, Kim H, Park K. Methionine Sulfoxide Reductase A Deficiency Exacerbates Cisplatin-Induced Nephrotoxicity via Increased Mitochondrial Damage and Renal Cell Death. Antioxid Redox Signal. 2017;27:727-741 pubmed publisher
    Methionine sulfoxide reductase A (MsrA), which is abundantly localized in the mitochondria, reduces methionine-S-sulfoxide, scavenging reactive oxygen species (ROS)...
  15. Lim J, You Z, Kim G, Levine R. Methionine sulfoxide reductase A is a stereospecific methionine oxidase. Proc Natl Acad Sci U S A. 2011;108:10472-7 pubmed publisher
    Methionine sulfoxide reductase A (MsrA) catalyzes the reduction of methionine sulfoxide to methionine and is specific for the S epimer of methionine sulfoxide...
  16. Zhang C, Jia P, Jia Y, Weissbach H, Webster K, Huang X, et al. Methionine sulfoxide reductase A (MsrA) protects cultured mouse embryonic stem cells from H2O2-mediated oxidative stress. J Cell Biochem. 2010;111:94-103 pubmed publisher
    Methionine sulfoxide reductase A (MsrA), a member of the Msr gene family, can reduce methionine sulfoxide residues in proteins formed by oxidation of methionine by reactive oxygen species (ROS)...
  17. Kwon T, Cho H, Kim U, Lee E, Oh S, Bok J, et al. Methionine sulfoxide reductase B3 deficiency causes hearing loss due to stereocilia degeneration and apoptotic cell death in cochlear hair cells. Hum Mol Genet. 2014;23:1591-601 pubmed publisher
    ..Taken together, our data suggest that MsrB3 plays an essential role in maintaining the integrity of hair cells, possibly explaining the pathogenesis of DFNB74 deafness in humans caused by MSRB3 deficiency. ..
  18. Oien D, Canello T, Gabizon R, Gasset M, Lundquist B, Burns J, et al. Detection of oxidized methionine in selected proteins, cellular extracts and blood serums by novel anti-methionine sulfoxide antibodies. Arch Biochem Biophys. 2009;485:35-40 pubmed
    ..It is suggested that anti-MetO-DZS18 antibodies can be applied in the identification of proteins that undergo methionine oxidation under oxidative stress, aging, or disease state conditions. ..
  19. Jia P, Zhang C, Jia Y, Webster K, Huang X, Kochegarov A, et al. Identification of a truncated form of Methionine Sulfoxide Reductase A expressed in mouse embryonic stem cells. J Biomed Sci. 2011;18:46 pubmed publisher
    Methionine Sulfoxide Reductase A (MsrA), an enzyme in the Msr gene family, is important in the cellular anti-oxidative stress defense mechanism...
  20. Kim G, Cole N, Lim J, Zhao H, Levine R. Dual sites of protein initiation control the localization and myristoylation of methionine sulfoxide reductase A. J Biol Chem. 2010;285:18085-94 pubmed publisher
    ..Lipidation of methionine sulfoxide reductase A occurs in the mouse, in transfected tissue culture cells, and even in a cell-free protein synthesis system. The physiologic role of myristoylation of MsrA remains to be elucidated.
  21. Henderson L, Doshi V, Blackman S, Naughton K, Pace R, Moskovitz J, et al. Variation in MSRA modifies risk of neonatal intestinal obstruction in cystic fibrosis. PLoS Genet. 2012;8:e1002580 pubmed publisher
    ..analysis of a locus that had previously been linked to MI and found that SNP haplotypes 5' to and within the MSRA gene were associated with MI (P?=?1.99 × 10(-5) to 1.08 × 10(-6); Bonferroni P?=?0.057 to 3.1 × 10(-3))...
  22. Bilsborough J, Van Pel A, Uyttenhove C, Boon T, Van den Eynde B. Identification of a second major tumor-specific antigen recognized by CTLs on mouse mastocytoma P815. J Immunol. 1999;162:3534-40 pubmed
    ..p. with syngeneic tumor cells (L1210) that were constructed to express B7-1 and P815E did not induce resistance against live P815, even though a strong anti-P815E CTL response was observed with splenocytes from immunized animals. ..
  23. Zhao H, Sun J, Deschamps A, Kim G, Liu C, Murphy E, et al. Myristoylated methionine sulfoxide reductase A protects the heart from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2011;301:H1513-8 pubmed publisher
    Methionine sulfoxide reductase A (MsrA) catalytically scavenges reactive oxygen species and also repairs oxidized methionines in proteins...
  24. Brennan L, Lee W, Giblin F, David L, Kantorow M. Methionine sulfoxide reductase A (MsrA) restores alpha-crystallin chaperone activity lost upon methionine oxidation. Biochim Biophys Acta. 2009;1790:1665-72 pubmed publisher
    ..Two proteins that cause cataract when deleted from the lens are methionine sulfoxide reductase A (MsrA) that repairs protein methionine sulfoxide (PMSO) oxidized proteins and alpha-crystallin which is a two-subunit (..
  25. Le D, Liang X, Fomenko D, Raza A, Chong C, Carlson B, et al. Analysis of methionine/selenomethionine oxidation and methionine sulfoxide reductase function using methionine-rich proteins and antibodies against their oxidized forms. Biochemistry. 2008;47:6685-94 pubmed publisher
    ..In cells, the resulting Met sulfoxides are reduced back to Met by stereospecific reductases MsrA and MsrB...
  26. Moskovitz J, Du F, Bowman C, Yan S. Methionine sulfoxide reductase A affects β-amyloid solubility and mitochondrial function in a mouse model of Alzheimer's disease. Am J Physiol Endocrinol Metab. 2016;310:E388-93 pubmed publisher
    ..effect of an in vivo methionine sulfoxidation of Aβ through ablation of the methionine sulfoxide reductase A (MsrA) in a mouse model of AD, a mouse that overexpresses amyloid precursor protein (APP) and Aβ in neurons...
  27. Cole N, Daniels M, Levine R, Kim G. Oxidative stress causes reversible changes in mitochondrial permeability and structure. Exp Gerontol. 2010;45:596-602 pubmed publisher
    ..The ability of reactive oxygen species to reversibly alter the permeability of the mitochondrial membrane provides a potential mechanism for communication within the cell such as between nucleus and mitochondria. ..
  28. Zhao H, Kim G, Levine R. Methionine sulfoxide reductase contributes to meeting dietary methionine requirements. Arch Biochem Biophys. 2012;522:37-43 pubmed publisher
    ..We conclude that the methionine sulfoxide reductases can provide methionine for growth in mice with limited intake of methionine, such as may occur in the wild. ..
  29. Lim J, Gruschus J, Ghesquière B, Kim G, Piszczek G, Tjandra N, et al. Characterization and solution structure of mouse myristoylated methionine sulfoxide reductase A. J Biol Chem. 2012;287:25589-95 pubmed publisher
    ..quot; We propose that this structure functions to enhance protein-protein interaction. ..
  30. Oien D, Ortiz A, Rittel A, Dobrowsky R, Johnson M, Levant B, et al. Dopamine D(2) receptor function is compromised in the brain of the methionine sulfoxide reductase A knockout mouse. J Neurochem. 2010;114:51-61 pubmed publisher
    ..We conclude that ablation of methionine sulfoxide reductase can affect dopamine signaling through altering dopamine D(2)-receptor physiology and may be related to symptoms associated with neurological disorders and diseases. ..
  31. Shin S, Yoon H, Chun Y, Shin H, Lee M, Oh G, et al. Arrest defective 1 regulates the oxidative stress response in human cells and mice by acetylating methionine sulfoxide reductase A. Cell Death Dis. 2014;5:e1490 pubmed publisher
    Methionine sulfoxide reductase A (MSRA) protects proteins from oxidation, and also helps remove reactive oxygen species (ROS) by recovering antioxidant enzymes inactivated by oxidation...
  32. Kwak G, Kim J, Kim H. Expression, subcellular localization, and antioxidant role of mammalian methionine sulfoxide reductases in Saccharomyces cerevisiae. BMB Rep. 2009;42:113-8 pubmed
    Despite the growing body of evidence suggesting a role for MsrA in antioxidant defense, little is currently known regarding the function of MsrB in cellular protection against oxidative stress...
  33. Zhang C, Jia P, Jia Y, Li Y, Webster K, Huang X, et al. Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells. J Cell Biochem. 2011;112:98-106 pubmed publisher
    Methionine sulfoxide reductases (Msr) belong to a gene family that contains one MsrA and three MsrBs (MsrB1, MsrB2, and MsrB3). We have identified all four of the genes that are expressed in mouse embryonic stem cell cultures...
  34. Singh M, Kwak G, Kim K, Kim H. Methionine sulfoxide reductase A protects hepatocytes against acetaminophen-induced toxicity via regulation of thioredoxin reductase 1 expression. Biochem Biophys Res Commun. 2017;487:695-701 pubmed publisher
    ..Methionine sulfoxide reductase A (MsrA) is an antioxidant and protein repair enzyme that specifically catalyzes the reduction of methionine S-sulfoxide ..
  35. Kim J, Choi S, Lee E, Kang Y, Kim H. Methionine sulfoxide reductase A attenuates heme oxygenase-1 induction through inhibition of Nrf2 activation. Arch Biochem Biophys. 2012;528:134-40 pubmed publisher
    Methionine sulfoxide reductase A (MsrA) functions as a protein repair enzyme by catalyzing the stereospecific reduction of methionine-S-sulfoxide to methionine...
  36. Singh M, Kim K, Kwak G, Baek S, Kim H. Methionine sulfoxide reductase A protects against lipopolysaccharide-induced septic shock via negative regulation of the proinflammatory responses. Arch Biochem Biophys. 2017;631:42-48 pubmed publisher
    Methionine sulfoxide reductase A (MsrA) is a major antioxidant enzyme that specifically catalyzes the reduction of methionine S-sulfoxide...
  37. Lee B, Péterfi Z, Hoffmann F, Moore R, Kaya A, Avanesov A, et al. MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation. Mol Cell. 2013;51:397-404 pubmed publisher
    ..More generally, our study shows that proteins can be regulated by reversible site-specific methionine-R-sulfoxidation. ..
  38. Styskal J, Nwagwu F, Watkins Y, Liang H, Richardson A, Musi N, et al. Methionine sulfoxide reductase A affects insulin resistance by protecting insulin receptor function. Free Radic Biol Med. 2013;56:123-32 pubmed publisher
    ..have found human obesity to be strongly correlated with polymorphisms near the methionine sulfoxide reductase A (MsrA) locus...
  39. Kwak G, Hwang K, Kim H. Analyses of methionine sulfoxide reductase activities towards free and peptidyl methionine sulfoxides. Arch Biochem Biophys. 2012;527:1-5 pubmed publisher
    There have been insufficient kinetic data that enable a direct comparison between free and peptide methionine sulfoxide reductase activities of either MsrB or MsrA...
  40. Ortiz A, Oien D, Moskovitz J, Johnson M. Quantification of reserve pool dopamine in methionine sulfoxide reductase A null mice. Neuroscience. 2011;177:223-9 pubmed publisher
    Methionine sulfoxide reductase A knockout (MsrA-/-) mice, which serve as a potential model for neurodegeneration, suffer from increased oxidative stress and have previously been found to have chronically elevated brain dopamine (DA) ..
  41. Lim J, Gruschus J, Kim G, Berlett B, Tjandra N, Levine R. A low pKa cysteine at the active site of mouse methionine sulfoxide reductase A. J Biol Chem. 2012;287:25596-601 pubmed publisher
    ..The low pK(a) renders the active site cysteine susceptible to oxidation to sulfenic acid by micromolar concentrations of hydrogen peroxide. This characteristic supports a role for methionine sulfoxide reductase in redox signaling. ..
  42. Nan C, Li Y, Jean Charles P, Chen G, Kreymerman A, Prentice H, et al. Deficiency of methionine sulfoxide reductase A causes cellular dysfunction and mitochondrial damage in cardiac myocytes under physical and oxidative stresses. Biochem Biophys Res Commun. 2010;402:608-13 pubmed publisher
    Methionine sulfoxide reductase A (MsrA) is an enzyme that reverses oxidation of methionine in proteins. Using a MsrA gene knockout (MsrA(-/-)) mouse model, we have investigated the role of MsrA in the heart...