Gene Symbol: Mbd1
Description: methyl-CpG binding domain protein 1
Alias: Cxxc3, PCM1, methyl-CpG-binding domain protein 1, methyl-CpG-binding protein MBD1
Species: mouse
Products:     Mbd1

Top Publications

  1. Jørgensen H, Ben Porath I, Bird A. Mbd1 is recruited to both methylated and nonmethylated CpGs via distinct DNA binding domains. Mol Cell Biol. 2004;24:3387-95 pubmed
    b>MBD1 is a vertebrate methyl-CpG binding domain protein (MBD) that can bring about repression of methylated promoter DNA sequences. Like other MBD proteins, MBD1 localizes to nuclear foci that in mice are rich in methyl-CpG...
  2. Allan A, Liang X, Luo Y, Pak C, Li X, Szulwach K, et al. The loss of methyl-CpG binding protein 1 leads to autism-like behavioral deficits. Hum Mol Genet. 2008;17:2047-57 pubmed publisher
    ..Our previous studies showed that the loss of Mbd1 led to reduced hippocampal neurogenesis and impaired learning in mice...
  3. Zhao X, Ueba T, Christie B, Barkho B, McConnell M, Nakashima K, et al. Mice lacking methyl-CpG binding protein 1 have deficits in adult neurogenesis and hippocampal function. Proc Natl Acad Sci U S A. 2003;100:6777-82 pubmed
    ..Methyl-CpG binding protein 1 (MBD1), a member of the methylated DNA-binding protein family, has been shown to bind methylated gene promoters and ..
  4. Liu C, Teng Z, Santistevan N, Szulwach K, Guo W, Jin P, et al. Epigenetic regulation of miR-184 by MBD1 governs neural stem cell proliferation and differentiation. Cell Stem Cell. 2010;6:433-44 pubmed publisher
    Methyl-CpG binding protein 1 (MBD1) regulates gene expression via a DNA methylation-mediated epigenetic mechanism...
  5. Li X, Barkho B, Luo Y, Smrt R, Santistevan N, Liu C, et al. Epigenetic regulation of the stem cell mitogen Fgf-2 by Mbd1 in adult neural stem/progenitor cells. J Biol Chem. 2008;283:27644-52 pubmed publisher
    ..We previously demonstrated that methylated CpG binding protein 1 (MBD1/Mbd1) is a transcriptional repressor of Fgf-2 and is enriched in adult brains...
  6. Sakamoto Y, Watanabe S, Ichimura T, Kawasuji M, Koseki H, Baba H, et al. Overlapping roles of the methylated DNA-binding protein MBD1 and polycomb group proteins in transcriptional repression of HOXA genes and heterochromatin foci formation. J Biol Chem. 2007;282:16391-400 pubmed
    ..We report that the DNA methylation-mediated repressor MBD1 interacts with Ring1b and hPc2, the major components of Polycomb repressive complex 1...
  7. Jobe E, Gao Y, Eisinger B, Mladucky J, Giuliani C, Kelnhofer L, et al. Methyl-CpG-Binding Protein MBD1 Regulates Neuronal Lineage Commitment through Maintaining Adult Neural Stem Cell Identity. J Neurosci. 2017;37:523-536 pubmed publisher
    Methyl-CpG-binding domain 1 (MBD1) belongs to a family of methyl-CpG-binding proteins that are epigenetic "readers" linking DNA methylation to transcriptional regulation...
  8. Varshney D, Vavrova Anderson J, Oler A, Cowling V, Cairns B, White R. SINE transcription by RNA polymerase III is suppressed by histone methylation but not by DNA methylation. Nat Commun. 2015;6:6569 pubmed publisher
    ..The data suggest that methylation of histones rather than DNA plays a dominant role in suppressing SINE transcription. ..
  9. McMurray E, Schmidt J. Identification of imprinting regulators at the Meg3 differentially methylated region. Genomics. 2012;100:184-94 pubmed publisher
    ..DNA-binding proteins that represent potential regulatory factors were identified in both ES cells and embryos. ..

More Information


  1. Carlone D, Hart S, Ladd P, Skalnik D. Cloning and characterization of the gene encoding the mouse homologue of CpG binding protein. Gene. 2002;295:71-7 pubmed
    ..In contrast to the human CGBP gene, which is located 800 bp upstream of the MBD1 gene, analysis of the murine CGBP gene locus failed to detect the murine MBD1 gene within several kilobases of the ..
  2. Waterfield M, Khan I, Cortez J, Fan U, Metzger T, Greer A, et al. The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance. Nat Immunol. 2014;15:258-65 pubmed publisher
    ..Here we identified two Aire-interacting proteins known to be involved in gene repression, ATF7ip and MBD1, that were required for Aire's targeting of loci encoding TSAs...
  3. Bierne H, Tham T, Batsche E, Dumay A, Leguillou M, Kernéis Golsteyn S, et al. Human BAHD1 promotes heterochromatic gene silencing. Proc Natl Acad Sci U S A. 2009;106:13826-31 pubmed publisher
    ..This nuclear protein interacts with HP1, MBD1, HDAC5, and several transcription factors...
  4. Hendrich B, Abbott C, McQueen H, Chambers D, Cross S, Bird A. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. Mamm Genome. 1999;10:906-12 pubmed
    DNA methylation is essential for murine development and is implicated in the control of gene expression. MeCP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of mammalian, nuclear proteins related by the presence in each of an amino acid ..
  5. Monnier P, Martinet C, Pontis J, Stancheva I, Ait Si Ali S, Dandolo L. H19 lncRNA controls gene expression of the Imprinted Gene Network by recruiting MBD1. Proc Natl Acad Sci U S A. 2013;110:20693-8 pubmed publisher
    ..Here, we identified the methyl-CpG-binding domain protein 1 MBD1 as a physical and functional partner of the H19 long noncoding RNA (lncRNA)...
  6. Mann W, Haaf T. Single cell RT-PCR on mouse embryos: a general approach for developmental biology. Methods Mol Biol. 2010;630:3-12 pubmed publisher
    ..It is tempting to speculate that cells expressing the reprogramming machinery have a higher developmental potential. ..
  7. Hendrich B, Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol. 1998;18:6538-47 pubmed
    Methylation at the DNA sequence 5'-CpG is required for mouse development. MeCP2 and MBD1 (formerly PCM1) are two known proteins that bind specifically to methylated DNA via a related amino acid motif and that can repress transcription...
  8. Rybak A, Fuchs H, Smirnova L, Brandt C, Pohl E, Nitsch R, et al. A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitment. Nat Cell Biol. 2008;10:987-93 pubmed publisher
  9. Liu C, Teng Z, McQuate A, Jobe E, Christ C, von Hoyningen Huene S, et al. An epigenetic feedback regulatory loop involving microRNA-195 and MBD1 governs neural stem cell differentiation. PLoS ONE. 2013;8:e51436 pubmed publisher
    ..Methyl-CpG binding protein 1 (MBD1), an important epigenetic regulator of adult neurogenesis, controls the proliferation and differentiation of adult ..
  10. May A, Kirchner R, Muller H, Hartmann P, El Hajj N, Tresch A, et al. Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres. Biol Reprod. 2009;80:194-202 pubmed publisher
    ..Immunolocalization of DNMT3A, MBD3, APEX1, and LIG3 in most or all nuclei of 40-60-cell embryos is a good indicator of functional activity of genes that are activated by the 16-cell stage...
  11. Katryniok C, Schnur N, Gillis A, von Knethen A, Sorg B, Looijenga L, et al. Role of DNA methylation and methyl-DNA binding proteins in the repression of 5-lipoxygenase promoter activity. Biochim Biophys Acta. 2010;1801:49-57 pubmed publisher
    ..Using ChIP assays, we found that the methyl-DNA binding proteins MBD1, MBD2 and MeCP2 bind to the methylated 5-LO core promoter in U937 cells...
  12. Craig J, Earle E, Canham P, Wong L, Anderson M, Choo K. Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns. Hum Mol Genet. 2003;12:3109-21 pubmed
    ..any of the pericentric and other major heterochromatic regions; and (b) heterochromatin-associated proteins (MeCP2, MBD1, MBD2, ATRX, HP1alpha, HDAC1, HDAC2, DNMT1 and DNMT3b), which colocalize with centromeric/pericentric ..
  13. Ruddock D Cruz N, Xue J, Wilson K, Heffernan C, Prashadkumar S, Cooney M, et al. Dynamic changes in the localization of five members of the methyl binding domain (MBD) gene family during murine and bovine preimplantation embryo development. Mol Reprod Dev. 2008;75:48-59 pubmed
    ..Most MBDs showed species-specificity in localization patterns and differences were found between individual MBDs. MBD1 localization is consistent with a novel role during MZT for both species...
  14. Muotri A, Marchetto M, Coufal N, Oefner R, Yeo G, Nakashima K, et al. L1 retrotransposition in neurons is modulated by MeCP2. Nature. 2010;468:443-6 pubmed publisher
    ..Our findings add a new level of complexity to the molecular events that can lead to neurological disorders...
  15. Wischnewski F, Friese O, Pantel K, Schwarzenbach H. Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters. Mol Cancer Res. 2007;5:749-59 pubmed
    ..mechanism underlying methylation-dependent repression, we examined the involvement of methyl-CpG binding proteins, MBD1, MBD2a, and MeCP2, in silencing of MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 genes...
  16. Reese B, Bachman K, Baylin S, Rountree M. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol. 2003;23:3226-36 pubmed
    ..such as histone deacetylases, to methylated DNA via a family of proteins called methyl-CpG binding proteins (MBD1 to -4)...
  17. Ohki I, Shimotake N, Fujita N, Jee J, Ikegami T, Nakao M, et al. Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA. Cell. 2001;105:487-97 pubmed
    ..We report here the solution structure of the MBD of the human methylation-dependent transcriptional regulator MBD1 bound to methylated DNA. DNA binding causes a loop in MBD1 to fold into a major and novel DNA binding interface...
  18. Nunes S, Ferralli J, Choi K, Brown Luedi M, Minet A, Chiquet Ehrismann R. The intracellular domain of teneurin-1 interacts with MBD1 and CAP/ponsin resulting in subcellular codistribution and translocation to the nuclear matrix. Exp Cell Res. 2005;305:122-32 pubmed
    ..The second interacting protein identified is the methyl-CpG binding protein MBD1. In the nucleus, the intracellular domain of teneurin-1 colocalizes with this transcriptional repressor in foci ..
  19. Wood K, Johnson B, Welsh S, Lee J, Cui Y, Krizman E, et al. Tagging methyl-CpG-binding domain proteins reveals different spatiotemporal expression and supports distinct functions. Epigenomics. 2016;8:455-73 pubmed publisher
    ..We analyzed behavioral phenotypes, generated biotin-tagged MBD1 and MBD2 knockin mice, and performed biochemical studies of MBD2-NuRD...
  20. Zhang P, Rausch C, Hastert F, Boneva B, Filatova A, Patil S, et al. Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. Nucleic Acids Res. 2017;45:7118-7136 pubmed publisher
    ..demonstrate that increased Tet1 heterochromatin localization and 5-methylcytosine oxidation are dependent on the CXXC3 domain of Mbd1, which recognizes unmethylated CpG dinucleotides...