Mad2l1

Summary

Gene Symbol: Mad2l1
Description: MAD2 mitotic arrest deficient-like 1
Alias: AA673185, MAD2, MAD2 (mitotic arrest deficient, homolog)-like 1, MAD2-like protein 1, mitotic arrest deficient 2-like protein 1, mitotic spindle assembly checkpoint protein MAD2A
Species: mouse

Top Publications

  1. ncbi Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2
    M Dobles
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Cell 101:635-45. 2000
  2. doi Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression
    Teruo Nakaya
    Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
    J Immunol 185:5180-7. 2010
  3. pmc Early aging-associated phenotypes in Bub3/Rae1 haploinsufficient mice
    Darren J Baker
    Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Cell Biol 172:529-40. 2006
  4. pmc Changing Mad2 levels affects chromosome segregation and spindle assembly checkpoint control in female mouse meiosis I
    Théodora Niault
    CNRS UMR7622 Biologie du Développement, Paris, France
    PLoS ONE 2:e1165. 2007
  5. pmc Loss of Cdc20 causes a securin-dependent metaphase arrest in two-cell mouse embryos
    Min Li
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell Biol 27:3481-8. 2007
  6. ncbi Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle poles
    Dong Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Reproduction 133:685-95. 2007
  7. pmc Generating chromosome instability through the simultaneous deletion of Mad2 and p53
    Aurora A Burds
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:11296-301. 2005
  8. pmc The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes
    Chizuko Tsurumi
    Max Planck Institut fuer Immunbiologie, Developmental Biology, Freiburg, Germany
    J Cell Biol 167:1037-50. 2004
  9. ncbi Heterozygous deletion of mitotic arrest-deficient protein 1 (MAD1) increases the incidence of tumors in mice
    Yoichi Iwanaga
    Molecular Virology Section, Laboratory of Molecular Microbiology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Cancer Res 67:160-6. 2007
  10. pmc A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint
    Rui Hong Wang
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:17108-13. 2004

Scientific Experts

  • Katja Wassmann
  • Chizuko Tsurumi
  • Rocio Sotillo
  • Robert Benezra
  • Qing Yuan Sun
  • Juan Manuel Schvartzman
  • Ya Hui Chi
  • Yi Hou
  • Peter K Sorger
  • Shen Yin
  • Anna Hupalowska
  • Floris Foijer
  • Ying Zhang
  • Yanchang Wei
  • Juan Xu
  • Luca L Fava
  • Sara L Rohrabaugh
  • Teruo Nakaya
  • Ying Chun Ouyang
  • Hal E Broxmeyer
  • Ping Zheng
  • Dale O Cowley
  • Kuan Teh Jeang
  • Tomohiro Yamauchi
  • Jerrold M Ward
  • Bo Xiong
  • Ching Yen Tsai
  • Da Yuan Chen
  • Min Li
  • Zbigniew Polanski
  • Shigeki Ito
  • Maria A Ciemerych
  • Théodora Niault
  • Dong Zhang
  • Yoichi Iwanaga
  • Jacek Z Kubiak
  • Jian Ying Wang
  • James Davenport
  • Marta Sikora-Polaczek
  • K Haller
  • Darren J Baker
  • M Dobles
  • Aurora A Burds
  • Hai Li
  • Rui Hong Wang
  • Craig A Hodges
  • Rumana Bahar
  • Giacomo Donati
  • Ian Smyth
  • Fiona M Watt
  • Allan Bradley
  • Emma Heath
  • Stephanie Z Xie
  • Tia DiTommaso
  • Katta Hautaviita
  • Jeffrey L Salisbury
  • Jan van Deursen
  • Paul J Galardy
  • Oded Foreman
  • Dennis A Wigle
  • George Vasmatzis
  • Farhad Kosari
  • L S Michel
  • M A Farmer
  • Giao Hangoc
  • Heide Schatten
  • Bian Hu
  • Xinxing Gao
  • Manuel Kaulich
  • R Benezra
  • Xuemei Tian
  • V Liberal
  • Saima Multi
  • Xingxu Huang
  • Jiankui Zhou
  • Courtney Coker
  • Qing Hua Zhang
  • P K Sorger
  • Mark R Kelley
  • Yinan Du
  • Pascal H G Duijf
  • Mo Li
  • Anna Santamaria
  • Junyu Ma
  • Zhen Bo Wang
  • Chun Hui Zhang
  • Zhao Jia Ge
  • Meizhi Wang
  • Liang Wei
  • Erich A Nigg

Detail Information

Publications37

  1. ncbi Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2
    M Dobles
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Cell 101:635-45. 2000
    ..To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in mice. We find that E5...
  2. doi Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression
    Teruo Nakaya
    Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
    J Immunol 185:5180-7. 2010
    ..In this study, using real-time RT-PCR and in situ RNA hybridization, we demonstrated that MAD2 mRNA export is selectively regulated by Pcid2/Thp1...
  3. pmc Early aging-associated phenotypes in Bub3/Rae1 haploinsufficient mice
    Darren J Baker
    Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Cell Biol 172:529-40. 2006
    ..Our findings suggest that early onset of aging-associated phenotypes in mice with mitotic checkpoint gene defects is linked to cellular senescence and activation of the p53 and p16 pathways rather than to aneuploidy...
  4. pmc Changing Mad2 levels affects chromosome segregation and spindle assembly checkpoint control in female mouse meiosis I
    Théodora Niault
    CNRS UMR7622 Biologie du Développement, Paris, France
    PLoS ONE 2:e1165. 2007
    ..Prolonged metaphase arrest due to overexpression of Mad2 has been shown to be deleterious to the ensuing anaphase, leading to the generation of aneuploidies and ..
  5. pmc Loss of Cdc20 causes a securin-dependent metaphase arrest in two-cell mouse embryos
    Min Li
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell Biol 27:3481-8. 2007
    ..Interestingly, Cdc20 and securin double mutant embryos could not maintain the metaphase arrest, suggesting a role of securin in preventing mitotic exit...
  6. ncbi Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle poles
    Dong Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Reproduction 133:685-95. 2007
    ..meiosis and its relationship with two major spindle checkpoint proteins, mitotic arrest-deficient (Mad) 1 and Mad2. Oocytes at various stages during the first meiosis were fixed and immunostained for dynein, Mad1, Mad2, ..
  7. pmc Generating chromosome instability through the simultaneous deletion of Mad2 and p53
    Aurora A Burds
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:11296-301. 2005
    ..and characterization of blastocysts and two independent mouse embryonic fibroblast lines carrying deletions in Mad2 and p53...
  8. pmc The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes
    Chizuko Tsurumi
    Max Planck Institut fuer Immunbiologie, Developmental Biology, Freiburg, Germany
    J Cell Biol 167:1037-50. 2004
    ..Similar results were obtained using dominant-negative forms of Mad2 and BubR1, as well as checkpoint resistant dominant APC/C activating forms of Cdc20...
  9. ncbi Heterozygous deletion of mitotic arrest-deficient protein 1 (MAD1) increases the incidence of tumors in mice
    Yoichi Iwanaga
    Molecular Virology Section, Laboratory of Molecular Microbiology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Cancer Res 67:160-6. 2007
    ..Our results indicate an essential MAD1 function in mouse development and correlate Mad1 haploinsufficiency with increased constitutive tumors...
  10. pmc A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint
    Rui Hong Wang
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:17108-13. 2004
    ..cells displayed decreased expression of a number of genes that are involved in the spindle checkpoint, including Mad2, which is a key component of spindle checkpoint that inhibits anaphase-promoting complex...
  11. ncbi MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells
    L S Michel
    Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 409:355-9. 2001
    ..Here we report that deletion of one MAD2 allele results in a defective mitotic checkpoint in both human cancer cells and murine primary embryonic ..
  12. ncbi Metaphase I arrest upon activation of the Mad2-dependent spindle checkpoint in mouse oocytes
    Katja Wassmann
    Laboratoire de Biologie Cellulaire du Developpement, UMR7622, CNRS, Universite Pierre et Marie Curie, 9 quai Saint Bernard, 75005 Paris, France
    Curr Biol 13:1596-608. 2003
    ..On the contrary, the existence of such a checkpoint during the first meiotic division in mammalian oocytes when homologous chromosomes are segregated has remained controversial...
  13. ncbi Differences in spindle association of the mitotic checkpoint protein Mad2 in mammalian spermatogenesis and oogenesis
    M Kallio
    Turku Center for Biotechnology, BioCity, 5th Floor, Tykistökatu 6B, Turku, FIN 20520, Finland
    Dev Biol 225:112-23. 2000
    We have investigated expression and subcellular localization of the spindle checkpoint protein Mad2 during rat and mouse spermatogenesis and in superovulated mouse oocytes...
  14. ncbi Simultaneous analysis of chromosomes and chromosome-associated proteins in mammalian oocytes and embryos
    Craig A Hodges
    Department of Genetics, Case Western Reserve University, Cleveland, Ohio, USA
    Chromosoma 111:165-9. 2002
    ....
  15. pmc Aurora-A kinase is essential for bipolar spindle formation and early development
    Dale O Cowley
    Department of Genetics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina 275991, USA
    Mol Cell Biol 29:1059-71. 2009
    ..Aurora-A conditional mutant mice provide a valuable system for further defining Aurora-A functions and for predicting effects of Aurora-A therapeutic intervention...
  16. pmc Spindle assembly checkpoint regulates mitotic cell cycle progression during preimplantation embryo development
    Yanchang Wei
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    PLoS ONE 6:e21557. 2011
    ..overexpression and RNA interference (RNAi) approaches, we analyzed the role of SAC components (Bub3, BubR1 and Mad2) in mouse preimplantation embryos...
  17. pmc Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint
    Luca L Fava
    Growth and Development, Biozentrum, University of Basel, Basel, Switzerland
    EMBO J 30:3322-36. 2011
    ..The SAC protein Mad2 can fold into two distinct conformers, open (O) and closed (C), and can asymmetrically dimerize...
  18. pmc USP44 regulates centrosome positioning to prevent aneuploidy and suppress tumorigenesis
    Ying Zhang
    Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Clin Invest 122:4362-74. 2012
    ..These functions required direct binding to the centriole protein centrin. Our data reveal a new role for the ubiquitin system in mitotic spindle regulation and underscore the importance of USP44 in the pathogenesis of human cancer...
  19. pmc Spindle checkpoint deficiency is tolerated by murine epidermal cells but not hair follicle stem cells
    Floris Foijer
    Mouse Genomics, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 110:2928-33. 2013
    ..Here we describe the use of a conditional KO of mouse Mad2, an essential component of the SAC signaling cascade, as a means to selectively induce chromosome instability and ..
  20. pmc Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis
    Ping Zheng
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:7473-8. 2009
    ..Concurrently, Filia is required for the placement of MAD2, an essential component of the SAC, to kinetochores to enable SAC function...
  21. pmc Mad2-induced chromosome instability leads to lung tumour relapse after oncogene withdrawal
    Rocio Sotillo
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 464:436-40. 2010
    ..Here we show that induction of chromosome instability by overexpression of the mitotic checkpoint gene Mad2 in mice does not affect the regression of Kras-driven lung tumours when Kras is inhibited...
  22. pmc Mad2 haploinsufficiency protects hematopoietic progenitor cells subjected to cell-cycle stress in vivo and to inhibition of redox function of Ape1/Ref-1 in vitro
    Sara L Rohrabaugh
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Exp Hematol 39:415-23. 2011
    Cell-cycle checkpoints guarantee movement through the cell cycle. Mitotic arrest deficiency 2 (Mad2), a mitotic checkpoint protein, appears crucial for generating the wait anaphase signal to prevent onset of anaphase...
  23. pmc Separase phosphosite mutation leads to genome instability and primordial germ cell depletion during oogenesis
    Juan Xu
    Model Animal Research Center, Nanjing University, Nanjing, China
    PLoS ONE 6:e18763. 2011
    ....
  24. pmc Mad2 is a critical mediator of the chromosome instability observed upon Rb and p53 pathway inhibition
    Juan Manuel Schvartzman
    Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, USA
    Cancer Cell 19:701-14. 2011
    ..It was recently shown that Rb pathway inhibition causes overexpression of the mitotic checkpoint gene Mad2, but whether Mad2 overexpression is required to generate CIN in this context is unknown...
  25. pmc Spindle assembly checkpoint and p53 deficiencies cooperate for tumorigenesis in mice
    Ya Hui Chi
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Int J Cancer 124:1483-9. 2009
    ..SAC in tumor development, mice heterozygously knocked out for the mitotic arrest deficient (Mad) genes Mad1 and/or Mad2 were mated with p53(+/) (-) mice...
  26. doi Metaphase I arrest in LT/Sv mouse oocytes involves the spindle assembly checkpoint
    Anna Hupalowska
    Department of Embryology, Institute of Zoology, Faculty of Biology, University of Warsaw, 02 096 Warsaw, Poland
    Biol Reprod 79:1102-10. 2008
    ..In contrast, there is prolonged localization of SAC proteins BUB1 and MAD2L1 (MAD2) at the metaphase I kinetochores in mutant oocytes compared with the wild-type...
  27. ncbi The N-terminus of rodent and human MAD1 confers species-specific stringency to spindle assembly checkpoint
    K Haller
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Oncogene 25:2137-47. 2006
    ..Our findings posit MAD1 as a species-specific determinant which influences the stringency of cellular response to microtubule depolymerization and spindle damage...
  28. ncbi The first mitosis of the mouse embryo is prolonged by transitional metaphase arrest
    Marta Sikora-Polaczek
    Department of Embryology, Institute of Zoology, Warsaw University, 02 096 Warsaw, Poland
    Biol Reprod 74:734-43. 2006
    ..The end of prometaphase is correlated with checkpoint inactivation and disappearance of MAD2L1 (MAD2) and RSN (CLIP-170) proteins from kinetochores...
  29. ncbi Spindle checkpoint function requires Mad2-dependent Cdc20 binding to the Mad3 homology domain of BubR1
    James Davenport
    Department of Pathology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105 2794, USA
    Exp Cell Res 312:1831-42. 2006
    ..A second Cdc20 binding site present between residues 1 and 477 is highly specific for Cdc20 already bound to Mad2. Mutation of a conserved lysine in this region weakened Cdc20 binding and correspondingly reduced checkpoint ..
  30. ncbi RNA Interference as a tool to study the function of MAD2 in mouse oocyte meiotic maturation
    Jian Ying Wang
    Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, PR China
    Mol Reprod Dev 74:116-24. 2007
    ..As a member of spindle checkpoint proteins, MAD2 takes a central role in the regulation of anaphase onset and genome integrity...
  31. pmc Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells
    Shigeki Ito
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA
    Blood 109:1923-30. 2007
    Mitotic arrest deficiency 2 (Mad2) is a component of mitotic spindle checkpoint proteins and is essential for accurate chromosome segregation. We investigated a role for Mad2 in hematopoiesis using Mad2-haploinsufficient (Mad2+/-) mice...
  32. ncbi Growth retardation, polyploidy, and multinucleation induced by Clast3, a novel cell cycle-regulated protein
    Rumana Bahar
    Division of Pathology, Chiba Cancer Center Research Institute, Chiba 260 8717, Japan
    J Biol Chem 277:40012-9. 2002
    ..These findings indicate that Clast3 is a novel cell cycle-regulated protein and that its constitutive overexpression induces polyploidy and multinucleation by interfering with the mitotic spindle checkpoint...
  33. pmc A B-Myb complex containing clathrin and filamin is required for mitotic spindle function
    Tomohiro Yamauchi
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Japan
    EMBO J 27:1852-62. 2008
    ..Thus, identification of the Myb-Clafi complex reveals a previously unrecognized function of B-Myb that may contribute to its role in chromosome stability, possibly, tumour suppression...
  34. doi BRCA1 is required for meiotic spindle assembly and spindle assembly checkpoint activation in mouse oocytes
    Bo Xiong
    State Key Laboratory of Reproductive Biology, Chinese Academy of Sciences, 100080 Beijing, China
    Biol Reprod 79:718-26. 2008
    ..Also, in BRCA1-depleted oocytes, gamma-tubulin dissociated from spindle poles and MAD2L1 failed to rebind to the kinetochores when exposed to nocodazole at metaphase I stage...
  35. pmc Hurp deficiency in mice leads to female infertility caused by an implantation defect
    Ching Yen Tsai
    Department of Life Sciences and Institute of Genome Sciences, National Yang Ming University, Taipei 112, Taiwan
    J Biol Chem 283:26302-6. 2008
    ..Our results demonstrate for the first time that HURP affects endometrial stromal proliferation during implantation but is dispensable during normal development in mice; specifically, HURP has an essential function in uterine biology...
  36. pmc HAT cofactor Trrap regulates the mitotic checkpoint by modulation of Mad1 and Mad2 expression
    Hai Li
    Group of Gene Environment Biology, International Agency for Research on Cancer IARC, Lyon, France
    EMBO J 23:4824-34. 2004
    ..checkpoint defects are caused by defective Trrap-mediated transcription of the mitotic checkpoint proteins Mad1 and Mad2. The mode of regulation by Trrap involves acetylation of histones H4 and H3 at the gene promoter of these mitotic ..
  37. pmc A major quantitative trait locus on chromosome 3 controls colitis severity in IL-10-deficient mice
    M A Farmer
    The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
    Proc Natl Acad Sci U S A 98:13820-5. 2001
    ..A human ortholog of the Chr. 3 QTL, if one exists, would map to Chr. 4q or 1p...