LIM kinase 2


Gene Symbol: LIM kinase 2
Description: LIM motif-containing protein kinase 2
Alias: Limk-2, whe, LIM domain kinase 2, LIM kinase 2
Species: mouse
Products:     LIM kinase 2

Top Publications

  1. Ikebe C, Ohashi K, Mizuno K. Identification of testis-specific (Limk2t) and brain-specific (Limk2c) isoforms of mouse LIM-kinase 2 gene transcripts. Biochem Biophys Res Commun. 1998;246:307-12 pubmed
    ..Testis- and brain-specific expression of Limk2t and Limk2c suggests specific roles in these tissues. ..
  2. Takahashi H, Koshimizu U, Nakamura T. A novel transcript encoding truncated LIM kinase 2 is specifically expressed in male germ cells undergoing meiosis. Biochem Biophys Res Commun. 1998;249:138-45 pubmed
    ..These results suggested the possibility that the tLimk2 product is involved in spermatogenesis, especially in meiotic and/or postmeiotic processes. ..
  3. Takahashi H, Koshimizu U, Miyazaki J, Nakamura T. Impaired spermatogenic ability of testicular germ cells in mice deficient in the LIM-kinase 2 gene. Dev Biol. 2002;241:259-72 pubmed
    ..Based on these findings, we propose that LIMK2, especially the testis-specific isoform tLIMK2, plays an important role in proper progression of spermatogenesis by regulation of cofilin activity and/or localization in germ cells. ..
  4. Acevedo K, Moussi N, Li R, Soo P, Bernard O. LIM kinase 2 is widely expressed in all tissues. J Histochem Cytochem. 2006;54:487-501 pubmed
    ..We have also demonstrated that endogenous LIMK1 and LIMK2 form heterodimers, and that LIMK2 does not always interact with the same proteins as LIMK1. ..
  5. Meng Y, Takahashi H, Meng J, Zhang Y, Lu G, Asrar S, et al. Regulation of ADF/cofilin phosphorylation and synaptic function by LIM-kinase. Neuropharmacology. 2004;47:746-54 pubmed
    ..These results indicate a critical role for the LIMK family in the regulation of ADF/cofilin and synaptic function in the brain. ..
  6. Ding Y, Milosavljevic T, Alahari S. Nischarin inhibits LIM kinase to regulate cofilin phosphorylation and cell invasion. Mol Cell Biol. 2008;28:3742-56 pubmed publisher
    ..In addition, knock-down of nischarin showed branched projection actin structures. Collectively these data indicate that nischarin siRNA may enhance random migration, resulting in stimulation of invasion. ..
  7. Mokalled M, Johnson A, Kim Y, Oh J, Olson E. Myocardin-related transcription factors regulate the Cdk5/Pctaire1 kinase cascade to control neurite outgrowth, neuronal migration and brain development. Development. 2010;137:2365-74 pubmed publisher
  8. Zhang Y, Qin F, Wu C, Li Y, Chen R, Shao M, et al. [Effects of LIMK2 RNA interference on the mechanosensitivity of c-fos gene in osteoblast]. Zhonghua Yi Xue Za Zhi. 2009;89:3143-6 pubmed
    ..409, P < 0.05) induced by fluid shear stress. Using RNAi against LIMK2 to inhibit the cytoskeleton reorganization can promote the expression of c-fos gene and thus enhance the mechanosensitivity of c-fos gene in osteoblast. ..
  9. Koike S, Keino Masu K, Ohto T, Sugiyama F, Takahashi S, Masu M. Autotaxin/lysophospholipase D-mediated lysophosphatidic acid signaling is required to form distinctive large lysosomes in the visceral endoderm cells of the mouse yolk sac. J Biol Chem. 2009;284:33561-70 pubmed publisher

More Information


  1. Yang Z, Tan S, Shen Y, Chen R, Wu C, Xu Y, et al. Inhibition of FSS-induced actin cytoskeleton reorganization by silencing LIMK2 gene increases the mechanosensitivity of primary osteoblasts. Bone. 2015;74:182-90 pubmed publisher
  2. Rice D, Hansen G, Liu F, Crist M, Newhouse M, Potter D, et al. Keratinocyte migration in the developing eyelid requires LIMK2. PLoS ONE. 2012;7:e47168 pubmed publisher
    ..These results demonstrate that LIMK2 activity is required for keratinocyte migration in the developing eyelid. ..
  3. Nekrasova T, Minden A. Role for p21-activated kinase PAK4 in development of the mammalian heart. Transgenic Res. 2012;21:797-811 pubmed publisher
    ..These results indicate that PAK4 functions, including control of actin dynamics, are necessary for normal development of the heart. ..
  4. Vardouli L, Moustakas A, Stournaras C. LIM-kinase 2 and cofilin phosphorylation mediate actin cytoskeleton reorganization induced by transforming growth factor-beta. J Biol Chem. 2005;280:11448-57 pubmed
    ..Our data define a novel pathway emanating from the TGF-beta type I receptor and leading to regulation of actin assembly, via the kinase LIMK2. ..
  5. Ybot Gonzalez P, Savery D, Gerrelli D, Signore M, Mitchell C, Faux C, et al. Convergent extension, planar-cell-polarity signalling and initiation of mouse neural tube closure. Development. 2007;134:789-99 pubmed
    ..These findings identify a cell-autonomous defect of convergent extension, requiring PCP signalling via RhoA-Rho kinase, during the development of severe neural tube defects in the mouse. ..
  6. Rosario M, Schuster S, J├╝ttner R, Parthasarathy S, Tarabykin V, Birchmeier W. Neocortical dendritic complexity is controlled during development by NOMA-GAP-dependent inhibition of Cdc42 and activation of cofilin. Genes Dev. 2012;26:1743-57 pubmed publisher
    ..Our findings define a novel cell-intrinsic mechanism to regulate dendritic branching and thus neuronal complexity in the cerebral cortex. ..
  7. Lourenco F, Munro J, Brown J, Cordero J, Stefanatos R, Strathdee K, et al. Reduced LIMK2 expression in colorectal cancer reflects its role in limiting stem cell proliferation. Gut. 2014;63:480-93 pubmed publisher
    Colorectal cancer (CRC) is a major contributor to cancer mortality and morbidity. LIM kinase 2 (LIMK2) promotes tumour cell invasion and metastasis...
  8. Koshimizu U, Takahashi H, Yoshida M, Nakamura T. cDNA cloning, genomic organization, and chromosomal localization of the mouse LIM motif-containing kinase gene, Limk2. Biochem Biophys Res Commun. 1997;241:243-50 pubmed
    ..Fluorescent in situ hybridization analysis showed the Limk2 gene were mapped to mouse chromosome 1D. These findings provide some important clues to the in vivo functions of LIM kinases. ..
  9. Liu A, Zhou Z, Dang R, Zhu Y, Qi J, He G, et al. Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization. J Cell Biol. 2016;212:449-63 pubmed publisher
    ..Our results provide a novel postsynaptic mechanism by which NLG1 regulates synapse development and function. ..
  10. Ikebe C, Ohashi K, Fujimori T, Bernard O, Noda T, Robertson E, et al. Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization, and tissue-specific expression of two alternatively initiated transcripts. Genomics. 1997;46:504-8 pubmed
    ..Limk2a and Limk2b transcripts were expressed at different ratios in a variety of mouse tissues. ..
  11. Palmer K, Fairfield H, Borgeia S, Curtain M, Hassan M, Dionne L, et al. Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology. Dev Biol. 2016;415:216-227 pubmed publisher
    ..Together with our detailed characterization, this work provides a valuable gene discovery resource for the craniofacial community, and a rich source of mouse models for further investigation. ..
  12. Andrews W, Zito A, Memi F, Jones G, Tamamaki N, Parnavelas J. Limk2 mediates semaphorin signalling in cortical interneurons migrating through the subpallium. Biol Open. 2013;2:277-82 pubmed publisher
    ..Our results reveal a previously unknown role for Limk2 in interneuron migration and Sema3A signalling. ..
  13. Jiang X, Wassif C, Backlund P, Song L, Holtzclaw L, Li Z, et al. Activation of Rho GTPases in Smith-Lemli-Opitz syndrome: pathophysiological and clinical implications. Hum Mol Genet. 2010;19:1347-57 pubmed publisher
    ..Developmental abnormalities of neuronal process formation may contribute to the neurocognitive deficits found in SLOS and may represent a potential target for therapeutic intervention. ..