Gene Symbol: Lgr5
Description: leucine rich repeat containing G protein coupled receptor 5
Alias: FEX, Gpr49, leucine-rich repeat-containing G-protein coupled receptor 5, G protein-coupled receptor 49, follicle expressed hormone, orphan G-protein coupled receptor FEX
Species: mouse
Products:     Lgr5

Top Publications

  1. van der Flier L, van Gijn M, Hatzis P, Kujala P, Haegebarth A, Stange D, et al. Transcription factor achaete scute-like 2 controls intestinal stem cell fate. Cell. 2009;136:903-12 pubmed publisher
    ..We recently demonstrated the existence of a long-lived pool of cycling stem cells defined by Lgr5 expression and intermingled with post-mitotic Paneth cells at crypt bottoms...
  2. Buczacki S, Zecchini H, Nicholson A, Russell R, Vermeulen L, Kemp R, et al. Intestinal label-retaining cells are secretory precursors expressing Lgr5. Nature. 2013;495:65-9 pubmed publisher
    ..These findings establish quiescent cells as an effective clonogenic reserve and provide a motivation for investigating their role in pathologies such as colorectal cancers and intestinal inflammation...
  3. VanDussen K, Samuelson L. Mouse atonal homolog 1 directs intestinal progenitors to secretory cell rather than absorptive cell fate. Dev Biol. 2010;346:215-23 pubmed publisher
    ..Thus, Math1 is a key effector directing multipotential precursors to adopt secretory and not absorptive cell fate. ..
  4. Zhu L, Gibson P, Currle D, Tong Y, Richardson R, Bayazitov I, et al. Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature. 2009;457:603-7 pubmed publisher
    ..reporter allele showed that Prom1(+) cells are located at the base of crypts in the small intestine, co-express Lgr5 (ref. 2), generate the entire intestinal epithelium, and are therefore the small intestinal stem cell...
  5. Hsu Y, Pasolli H, Fuchs E. Dynamics between stem cells, niche, and progeny in the hair follicle. Cell. 2011;144:92-105 pubmed publisher
  6. Arnold K, Sarkar A, Yram M, Polo J, Bronson R, Sengupta S, et al. Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice. Cell Stem Cell. 2011;9:317-29 pubmed publisher
    ..Thus, our results identify Sox2 expression in numerous adult endodermal and ectodermal stem cell compartments, which are critical for normal tissue regeneration and survival. ..
  7. Takeda N, Jain R, LeBoeuf M, Wang Q, Lu M, Epstein J. Interconversion between intestinal stem cell populations in distinct niches. Science. 2011;334:1420-4 pubmed publisher
    ..Conversely, CBCs can give rise to +4 Hopx-positive cells. These findings demonstrate a bidirectional lineage relationship between active and quiescent stem cells in their niches. ..
  8. Barker N, Ridgway R, van Es J, van de Wetering M, Begthel H, van den Born M, et al. Crypt stem cells as the cells-of-origin of intestinal cancer. Nature. 2009;457:608-11 pubmed publisher
    ..As in most cancers, the cell of origin has remained elusive. In a previously established Lgr5 (leucine-rich-repeat containing G-protein-coupled receptor 5) knockin mouse model, a tamoxifen-inducible Cre ..
  9. Munoz J, Stange D, Schepers A, van de Wetering M, Koo B, Itzkovitz S, et al. The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent '+4' cell markers. EMBO J. 2012;31:3079-91 pubmed publisher
    ..Here, we combine transcriptomics with proteomics to define a definitive molecular signature for Lgr5(+) CBC cells...

More Information


  1. Barker N, Huch M, Kujala P, van de Wetering M, Snippert H, van Es J, et al. Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell. 2010;6:25-36 pubmed publisher
    ..The Wnt target gene Lgr5 marks stem cells in the small intestine, colon, and hair follicle...
  2. Shi F, Kempfle J, Edge A. Wnt-responsive Lgr5-expressing stem cells are hair cell progenitors in the cochlea. J Neurosci. 2012;32:9639-48 pubmed publisher
    ..We show here that Lgr5, a marker for adult stem cells, was expressed in a subset of supporting cells in the newborn and adult murine ..
  3. Sato T, van Es J, Snippert H, Stange D, Vries R, van den Born M, et al. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature. 2011;469:415-8 pubmed publisher
    Homeostasis of self-renewing small intestinal crypts results from neutral competition between Lgr5 stem cells, which are small cycling cells located at crypt bottoms...
  4. Kasper M, Jaks V, Are A, Bergström A, Schwäger A, Svärd J, et al. Wounding enhances epidermal tumorigenesis by recruiting hair follicle keratinocytes. Proc Natl Acad Sci U S A. 2011;108:4099-104 pubmed publisher
  5. Kozar S, Morrissey E, Nicholson A, van der Heijden M, Zecchini H, Kemp R, et al. Continuous clonal labeling reveals small numbers of functional stem cells in intestinal crypts and adenomas. Cell Stem Cell. 2013;13:626-33 pubmed publisher
    ..These findings suggest that the bulk of tumor stem cell divisions serve only to replace stem cell loss, with rare clonal victors driving gland repopulation and tumor growth. ..
  6. Leushacke M, Barker N. Lgr5 and Lgr6 as markers to study adult stem cell roles in self-renewal and cancer. Oncogene. 2012;31:3009-22 pubmed publisher
    ..However, the 7-transmembrane receptor, Lgr5 has recently gained prominence as a marker of Wnt-regulated adult stem cell populations in the hair-follicle, ..
  7. Metcalfe C, Kljavin N, Ybarra R, De Sauvage F. Lgr5+ stem cells are indispensable for radiation-induced intestinal regeneration. Cell Stem Cell. 2014;14:149-59 pubmed publisher
    The intestinal epithelium continually self-renews and can rapidly regenerate after damage. Lgr5 marks mitotically active intestinal stem cells (ISCs)...
  8. Yee K, Li Y, Redding K, Iwatsuki K, Margolskee R, Jiang P. Lgr5-EGFP marks taste bud stem/progenitor cells in posterior tongue. Stem Cells. 2013;31:992-1000 pubmed publisher
    Until recently, reliable markers for adult stem cells have been lacking for many regenerative mammalian tissues. Lgr5 (leucine-rich repeat-containing G-protein-coupled receptor 5) has been identified as a marker for adult stem cells in ..
  9. Lei N, Jabaji Z, Wang J, Joshi V, Brinkley G, Khalil H, et al. Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells. PLoS ONE. 2014;9:e84651 pubmed publisher
    ..The addition of ISEMFs in intestinal epithelial culture therefore recapitulates a critical element of the intestinal stem cell niche and allows for its experimental interrogation and biodesign-driven manipulation. ..
  10. Sato T, Vries R, Snippert H, van de Wetering M, Barker N, Stange D, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009;459:262-5 pubmed publisher
    ..We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts...
  11. Schwitalla S, Fingerle A, Cammareri P, Nebelsiek T, Göktuna S, Ziegler P, et al. Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. Cell. 2013;152:25-38 pubmed publisher
    ..Thus, our data support the concept of bidirectional conversion and highlight the importance of inflammatory signaling for dedifferentiation and generation of tumor-initiating cells in vivo. ..
  12. van Es J, Sato T, van de Wetering M, Lyubimova A, Yee Nee A, Gregorieff A, et al. Dll1+ secretory progenitor cells revert to stem cells upon crypt damage. Nat Cell Biol. 2012;14:1099-1104 pubmed publisher
    b>Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of immediate stem cell daughters...
  13. Schepers A, Snippert H, Stange D, van den Born M, van Es J, van de Wetering M, et al. Lineage tracing reveals Lgr5+ stem cell activity in mouse intestinal adenomas. Science. 2012;337:730-5 pubmed publisher
    ..retracing" using the multicolor Cre-reporter R26R-Confetti, we demonstrate that the crypt stem cell marker Lgr5 (leucine-rich repeat-containing heterotrimeric guanine nucleotide-binding protein-coupled receptor 5) also marks a ..
  14. Nam K, O Neal R, Coffey R, Finke P, Barker N, Goldenring J. Spasmolytic polypeptide-expressing metaplasia (SPEM) in the gastric oxyntic mucosa does not arise from Lgr5-expressing cells. Gut. 2012;61:1678-85 pubmed publisher
    ..that cells demonstrating transcriptional activity for leucine-rich repeat containing G-protein-coupled receptor 5 (Lgr5) in the intestine, colon and gastric antrum function as adult stem cells...
  15. Yan K, Chia L, Li X, Ootani A, Su J, Lee J, et al. The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations. Proc Natl Acad Sci U S A. 2012;109:466-71 pubmed publisher
    ..Bmi1 and Lgr5 have been independently identified to mark long-lived multipotent ISCs by lineage tracing in mice; however, the ..
  16. Grachtchouk M, Pero J, Yang S, Ermilov A, Michael L, Wang A, et al. Basal cell carcinomas in mice arise from hair follicle stem cells and multiple epithelial progenitor populations. J Clin Invest. 2011;121:1768-81 pubmed publisher
  17. Garcia M, Ghiani M, Lefort A, Libert F, Strollo S, Vassart G. LGR5 deficiency deregulates Wnt signaling and leads to precocious Paneth cell differentiation in the fetal intestine. Dev Biol. 2009;331:58-67 pubmed publisher
    The orphan Leucine-rich repeat G protein-coupled receptor 5 (LGR5/GPR49), a target of Wnt signaling, is a marker of adult intestinal stem cells (SC)...
  18. VanDussen K, Carulli A, Keeley T, Patel S, Puthoff B, Magness S, et al. Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells. Development. 2012;139:488-97 pubmed publisher
    ..Our findings suggest that Notch targets distinct progenitor cell populations to maintain adult intestinal stem cells and to regulate cell fate choice to control epithelial cell homeostasis. ..
  19. Furuyama K, Kawaguchi Y, Akiyama H, Horiguchi M, Kodama S, Kuhara T, et al. Continuous cell supply from a Sox9-expressing progenitor zone in adult liver, exocrine pancreas and intestine. Nat Genet. 2011;43:34-41 pubmed publisher
    ..These results suggest interdependence between the structure and homeostasis of endodermal organs, with Sox9 expression being linked to progenitor status. ..
  20. Kim T, Escudero S, Shivdasani R. Intact function of Lgr5 receptor-expressing intestinal stem cells in the absence of Paneth cells. Proc Natl Acad Sci U S A. 2012;109:3932-7 pubmed publisher
    ..b>Lgr5 and Bmi1 are two molecular markers of crypt-cell populations that replenish all lineages over time and hence ..
  21. Snippert H, Haegebarth A, Kasper M, Jaks V, van Es J, Barker N, et al. Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin. Science. 2010;327:1385-9 pubmed publisher
    ..We generated knock-in alleles of murine Lgr6, a close relative of the Lgr5 stem cell gene. Lgr6 was expressed in the earliest embryonic hair placodes...
  22. Shi F, Hu L, Edge A. Generation of hair cells in neonatal mice by ?-catenin overexpression in Lgr5-positive cochlear progenitors. Proc Natl Acad Sci U S A. 2013;110:13851-6 pubmed publisher
    ..We recently identified leucine-rich repeat containing, G-protein-coupled receptor 5 (Lgr5)-expressing cochlear supporting cells with the capacity for self-renewal and hair cell differentiation in vitro...
  23. Montgomery R, Carlone D, Richmond C, Farilla L, Kranendonk M, Henderson D, et al. Mouse telomerase reverse transcriptase (mTert) expression marks slowly cycling intestinal stem cells. Proc Natl Acad Sci U S A. 2011;108:179-84 pubmed publisher
    The intestinal epithelium is maintained by a population of rapidly cycling (Lgr5(+)) intestinal stem cells (ISCs)...
  24. Hirata A, Utikal J, Yamashita S, Aoki H, Watanabe A, Yamamoto T, et al. Dose-dependent roles for canonical Wnt signalling in de novo crypt formation and cell cycle properties of the colonic epithelium. Development. 2013;140:66-75 pubmed publisher
    ..We show that elevated levels of Wnt signalling induce the amplification of Lgr5+ cells, which is accompanied by crypt fission and a reduction in cell proliferation among progenitor cells...
  25. Chivukula R, Shi G, Acharya A, Mills E, Zeitels L, Anandam J, et al. An essential mesenchymal function for miR-143/145 in intestinal epithelial regeneration. Cell. 2014;157:1104-16 pubmed publisher
    ..These results provide important insights into the regulation of epithelial wound healing and argue against a cell-autonomous tumor suppressor role for miR-143/145 in colon cancer...
  26. Huch M, Dorrell C, Boj S, van Es J, Li V, van de Wetering M, et al. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature. 2013;494:247-50 pubmed publisher
    The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles...
  27. Stange D, Koo B, Huch M, Sibbel G, Basak O, Lyubimova A, et al. Differentiated Troy+ chief cells act as reserve stem cells to generate all lineages of the stomach epithelium. Cell. 2013;155:357-68 pubmed publisher
    ..These observations challenge the notion that stem cell hierarchies represent a "one-way street." ..
  28. Mustata R, Van Loy T, Lefort A, Libert F, Strollo S, Vassart G, et al. Lgr4 is required for Paneth cell differentiation and maintenance of intestinal stem cells ex vivo. EMBO Rep. 2011;12:558-64 pubmed publisher
    Gene inactivation of the orphan G protein-coupled receptor LGR4, a paralogue of the epithelial-stem-cell marker LGR5, results in a 50% decrease in epithelial cell proliferation and an 80% reduction in terminal differentiation of Paneth ..
  29. Jaks V, Barker N, Kasper M, van Es J, Snippert H, Clevers H, et al. Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat Genet. 2008;40:1291-9 pubmed publisher
    In mouse hair follicles, a group of quiescent cells in the bulge is believed to have stem cell activity. Lgr5, a marker of intestinal stem cells, is expressed in actively cycling cells in the bulge and secondary germ of telogen hair ..
  30. Huch M, Bonfanti P, Boj S, Sato T, Loomans C, van de Wetering M, et al. Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. EMBO J. 2013;32:2708-21 pubmed publisher
    b>Lgr5 marks adult stem cells in multiple adult organs and is a receptor for the Wnt-agonistic R-spondins (RSPOs)...
  31. Powell A, Wang Y, Li Y, Poulin E, Means A, Washington M, et al. The pan-ErbB negative regulator Lrig1 is an intestinal stem cell marker that functions as a tumor suppressor. Cell. 2012;149:146-58 pubmed publisher
    ..Transcriptome profiling of Lrig1(+) colonic stem cells differs markedly from the profiling of highly proliferative, Lgr5(+) colonic stem cells; genes upregulated in the Lrig1(+) population include those involved in cell cycle repression ..
  32. Haegebarth A, Clevers H. Wnt signaling, lgr5, and stem cells in the intestine and skin. Am J Pathol. 2009;174:715-21 pubmed publisher
    ..The Wnt target gene Lgr5 has been recently identified as a novel stem cell marker of the intestinal epithelium and the hair follicle...
  33. Zhou W, Geng Z, Spence J, Geng J. Induction of intestinal stem cells by R-spondin 1 and Slit2 augments chemoradioprotection. Nature. 2013;501:107-11 pubmed publisher
    ..Therefore Rspo1 and Slit2 may act as therapeutic adjuvants to enhance host tolerance to aggressive chemoradiotherapy for eradicating metastatic cancers. ..
  34. Van Keymeulen A, Rocha A, Ousset M, Beck B, Bouvencourt G, Rock J, et al. Distinct stem cells contribute to mammary gland development and maintenance. Nature. 2011;479:189-93 pubmed publisher
  35. Fordham R, Yui S, Hannan N, Soendergaard C, Madgwick A, Schweiger P, et al. Transplantation of expanded fetal intestinal progenitors contributes to colon regeneration after injury. Cell Stem Cell. 2013;13:734-44 pubmed publisher
    ..Established cultures of mouse fetal intestinal progenitors express lower levels of Lgr5 than mature progenitors and propagate in the presence of the Wnt antagonist Dkk1, and new cultures can be induced ..
  36. Zhu Y, Huang Y, Kek C, Bulavin D. Apoptosis differently affects lineage tracing of Lgr5 and Bmi1 intestinal stem cell populations. Cell Stem Cell. 2013;12:298-303 pubmed publisher
    ..We found that about 40% of apoptotic cells were Lgr5-positive whereas Bmi1-positive ISCs became sensitive to tamoxifen upon entering a proliferative state...
  37. Tanese K, Fukuma M, Yamada T, Mori T, Yoshikawa T, Watanabe W, et al. G-protein-coupled receptor GPR49 is up-regulated in basal cell carcinoma and promotes cell proliferation and tumor formation. Am J Pathol. 2008;173:835-43 pubmed publisher
    ..formation in BCC, we screened a DNA microarray database of human BCC cases; the orphan G-protein-coupled receptor GPR49 was found to be up-regulated in all cases...
  38. Sakamori R, Das S, Yu S, Feng S, Stypulkowski E, Guan Y, et al. Cdc42 and Rab8a are critical for intestinal stem cell division, survival, and differentiation in mice. J Clin Invest. 2012;122:1052-65 pubmed publisher
  39. de Lau W, Barker N, Low T, Koo B, Li V, Teunissen H, et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature. 2011;476:293-7 pubmed publisher
    The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven proliferative compartments...
  40. Snippert H, van der Flier L, Sato T, van Es J, van den Born M, Kroon Veenboer C, et al. Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Cell. 2010;143:134-44 pubmed publisher
    Intestinal stem cells, characterized by high Lgr5 expression, reside between Paneth cells at the small intestinal crypt base and divide every day...
  41. Wong V, Stange D, Page M, Buczacki S, Wabik A, Itami S, et al. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat Cell Biol. 2012;14:401-8 pubmed publisher
    ..Our findings reveal ErbB activation as a strong inductive signal for stem-cell proliferation. This has implications for our understanding of ErbB signalling in tissue development and maintenance and the progression of malignant disease. ..
  42. Barker N, van Es J, Kuipers J, Kujala P, van den Born M, Cozijnsen M, et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007;449:1003-7 pubmed
    ..Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5, also known as Gpr49) was selected from a panel of intestinal Wnt target genes for its restricted crypt expression...
  43. Ritsma L, Ellenbroek S, Zomer A, Snippert H, De Sauvage F, Simons B, et al. Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging. Nature. 2014;507:362-365 pubmed publisher
    ..In addition to the Lgr5 marker, intestinal stem cells have been associated with other markers that are expressed heterogeneously within the ..
  44. Chai R, Kuo B, Wang T, Liaw E, Xia A, Jan T, et al. Wnt signaling induces proliferation of sensory precursors in the postnatal mouse cochlea. Proc Natl Acad Sci U S A. 2012;109:8167-72 pubmed publisher
    ..b>Lgr5, a Wnt target gene, marks distinct supporting cell types in the neonatal cochlea...
  45. Itzkovitz S, Lyubimova A, Blat I, Maynard M, van Es J, LEES J, et al. Single-molecule transcript counting of stem-cell markers in the mouse intestine. Nat Cell Biol. 2011;14:106-14 pubmed publisher
    ..We find that the expression of all markers overlaps at crypt-base cells. This co-expression includes Lgr5, Bmi1 and mTert, genes previously suggested to mark distinct stem cells...
  46. Tian H, Biehs B, Warming S, Leong K, Rangell L, Klein O, et al. A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011;478:255-9 pubmed publisher
    ..One pool consists of columnar Lgr5-expressing cells that cycle rapidly and are present predominantly at the crypt base...
  47. Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, et al. EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins. Dev Biol. 2012;371:136-45 pubmed publisher
    ..Finally, the barrier function of the intestinal epithelium was impaired in the mutant animals. These results suggest that EpCAM contributes to formation of intestinal barrier by recruiting claudins to cell-cell junctions. ..
  48. Sakitani K, Hayakawa Y, Deng H, Ariyama H, Kinoshita H, Konishi M, et al. CXCR4-expressing Mist1+ progenitors in the gastric antrum contribute to gastric cancer development. Oncotarget. 2017;8:111012-111025 pubmed publisher
    ..population in the corpus, comprise long-lived progenitors that reside within the antral isthmus above Lgr5+ or CCK2R+ cells...
  49. Wang T, Chai R, Kim G, Pham N, Jansson L, Nguyen D, et al. Lgr5+ cells regenerate hair cells via proliferation and direct transdifferentiation in damaged neonatal mouse utricle. Nat Commun. 2015;6:6613 pubmed publisher
    ..Here we show that HC damage in neonatal mouse utricle activates the Wnt target gene Lgr5 in striolar supporting cells...
  50. Gracz A, Williamson I, Roche K, Johnston M, Wang F, Wang Y, et al. A high-throughput platform for stem cell niche co-cultures and downstream gene expression analysis. Nat Cell Biol. 2015;17:340-9 pubmed publisher
    ..MRAs have broad applicability to assaying stem cell-niche interactions and organoid development, and serve as a high-throughput culture platform to interrogate gene expression at early stages of stem cell fate choices. ..
  51. Boumahdi S, Driessens G, Lapouge G, Rorive S, Nassar D, Le Mercier M, et al. SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma. Nature. 2014;511:246-50 pubmed publisher
    ..We demonstrate that SOX2, by marking and regulating the functions of skin tumour-initiating cells and CSCs, establishes a continuum between tumour initiation and progression in primary skin tumours. ..
  52. Rad R, Cadiñanos J, Rad L, Varela I, Strong A, Kriegl L, et al. A genetic progression model of Braf(V600E)-induced intestinal tumorigenesis reveals targets for therapeutic intervention. Cancer Cell. 2013;24:15-29 pubmed publisher
    ..Finally, systematic drug screening revealed sensitivity of this CRC subtype to targeted therapeutics, including Mek or combinatorial PI3K/Braf inhibition. ..
  53. Neal M, Sodhi C, Jia H, Dyer M, Egan C, Yazji I, et al. Toll-like receptor 4 is expressed on intestinal stem cells and regulates their proliferation and apoptosis via the p53 up-regulated modulator of apoptosis. J Biol Chem. 2012;287:37296-308 pubmed publisher
    ..Using flow cytometry and fluorescent in situ hybridization for the intestinal stem cell marker Lgr5, we demonstrate that TLR4 is expressed on the Lgr5-positive intestinal stem cells...
  54. Feng Y, Sentani K, Wiese A, Sands E, Green M, Bommer G, et al. Sox9 induction, ectopic Paneth cells, and mitotic spindle axis defects in mouse colon adenomatous epithelium arising from conditional biallelic Apc inactivation. Am J Pathol. 2013;183:493-503 pubmed publisher
    ..mitotic spindle misorientation, ?-catenin nuclear localization, and induction of the intestinal stem cell markers Lgr5 and Musashi-1 and the Sox9 transcription factor...
  55. Zhao B, Qi Z, Li Y, Wang C, Fu W, Chen Y. The non-muscle-myosin-II heavy chain Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells. Nat Commun. 2015;6:7166 pubmed publisher
    b>Lgr5+ stem cells are crucial to gut epithelium homeostasis, and therapies targeting these cells hold promise for treatment of gastrointestinal diseases...
  56. Peck B, Mah A, Pitman W, Ding S, Lund P, Sethupathy P. Functional Transcriptomics in Diverse Intestinal Epithelial Cell Types Reveals Robust MicroRNA Sensitivity in Intestinal Stem Cells to Microbial Status. J Biol Chem. 2017;292:2586-2600 pubmed publisher
    ..gene and miRNA expression ex vivo enteroids, we demonstrate that we can knock down gene expression in Lgr5+ IESCs...
  57. Qi Z, Li Y, Zhao B, Xu C, Liu Y, Li H, et al. BMP restricts stemness of intestinal Lgr5+ stem cells by directly suppressing their signature genes. Nat Commun. 2017;8:13824 pubmed publisher
    The intestinal epithelium possesses a remarkable self-renewal ability, which is mediated by actively proliferating Lgr5+ stem cells...
  58. Chai R, Xia A, Wang T, Jan T, Hayashi T, Bermingham McDonogh O, et al. Dynamic expression of Lgr5, a Wnt target gene, in the developing and mature mouse cochlea. J Assoc Res Otolaryngol. 2011;12:455-69 pubmed publisher
    ..b>Lgr5, a member of the G protein-coupled receptor family, is a Wnt target in the gastrointestinal and integumentary ..
  59. Niessen M, Scott J, Zielinski J, Vorhagen S, Sotiropoulou P, Blanpain C, et al. aPKC? controls epidermal homeostasis and stem cell fate through regulation of division orientation. J Cell Biol. 2013;202:887-900 pubmed publisher
  60. Dzama M, Nigmatullina L, Sayols S, Kreim N, Soshnikova N. Distinct populations of embryonic epithelial progenitors generate Lgr5+ intestinal stem cells. Dev Biol. 2017;432:258-264 pubmed publisher
    ..Cell fate mapping analysis revealed that multiple early embryonic epithelial progenitors give rise to Lgr5+ ISCs...