Gene Symbol: Hyou1
Description: hypoxia up-regulated 1
Alias: AI415631, CBP-140, Cab140, Grp170, Orp150, hypoxia up-regulated protein 1, 140 kDa Ca(2+)-binding protein, 150-kDa oxygen regulated protein, 170 kDa glucose regulated protein GRP170, GRP-170, calcium binding protein 140, calcium binding protein, 140 kDa
Species: mouse
Products:     Hyou1

Top Publications

  1. Kobayashi T, Ohta Y. 150-kD oxygen-regulated protein is an essential factor for insulin release. Pancreas. 2005;30:299-306 pubmed
    This study was designed to show the induction and function of 150-kD oxygen-regulated protein (ORP150) in insulin secretion in vitro...
  2. Wang Y, Wu Z, Li D, Wang D, Wang X, Feng X, et al. Involvement of oxygen-regulated protein 150 in AMP-activated protein kinase-mediated alleviation of lipid-induced endoplasmic reticulum stress. J Biol Chem. 2011;286:11119-31 pubmed publisher
    ..active AMPK overexpression induced the expression of an ER-associated chaperone, 150-kDa oxygen-regulated protein (ORP150), at both the mRNA and protein levels in hepatocytes...
  3. Tamatani M, Matsuyama T, Yamaguchi A, Mitsuda N, Tsukamoto Y, Taniguchi M, et al. ORP150 protects against hypoxia/ischemia-induced neuronal death. Nat Med. 2001;7:317-23 pubmed
    Oxygen-regulated protein 150 kD (ORP150) is a novel endoplasmic-reticulum-associated chaperone induced by hypoxia/ischemia...
  4. Kitao Y, Ozawa K, Miyazaki M, Tamatani M, Kobayashi T, Yanagi H, et al. Expression of the endoplasmic reticulum molecular chaperone (ORP150) rescues hippocampal neurons from glutamate toxicity. J Clin Invest. 2001;108:1439-50 pubmed
    ..We show that an inducible chaperone present in endoplasmic reticulum (ER), the 150-kDa oxygen-regulated protein (ORP150), is expressed both in the human brain after seizure attack and in mouse hippocampus after kainate administration...
  5. Kobayashi T, Takita Y, Suzuki A, Katsu Y, Iguchi T, Ohta Y. Vacuolar degeneration of skeletal muscle in transgenic mice overexpressing ORP150. J Vet Med Sci. 2008;70:115-8 pubmed
    b>ORP150 is a hypoxic stress-induced protein located in the endoplasmic reticulum. Transgenic mice overexpressing ORP150 (ORP-Tg) exhibit vacuolar degeneration in the heart...
  6. Kitao Y, Hashimoto K, Matsuyama T, Iso H, Tamatani T, Hori O, et al. ORP150/HSP12A regulates Purkinje cell survival: a role for endoplasmic reticulum stress in cerebellar development. J Neurosci. 2004;24:1486-96 pubmed
    ..b>ORP150 (oxygen-regulated protein 150)/HSP12A (heat shock protein 12A), a novel stress protein located in the ER, was ..
  7. Behnke J, Hendershot L. The large Hsp70 Grp170 binds to unfolded protein substrates in vivo with a regulation distinct from conventional Hsp70s. J Biol Chem. 2014;289:2899-907 pubmed publisher
    ..The ER also possesses a single large Hsp70, the glucose-regulated protein of 170 kDa (Grp170). Like BiP it is an essential protein, but its cellular functions are not well understood...
  8. Gao P, Sun X, Chen X, Wang Y, Foster B, Subjeck J, et al. Secretable chaperone Grp170 enhances therapeutic activity of a novel tumor suppressor, mda-7/IL-24. Cancer Res. 2008;68:3890-8 pubmed publisher
    ..mda-7) and a secretable form of endoplasmic reticulum resident chaperone grp170 (Ad.sgrp170), a potent immunostimulatory adjuvant and antigen carrier. Intratumoral administration of Ad...
  9. Arnouk H, Zynda E, Wang X, Hylander B, Manjili M, Repasky E, et al. Tumour secreted grp170 chaperones full-length protein substrates and induces an adaptive anti-tumour immune response in vivo. Int J Hyperthermia. 2010;26:366-75 pubmed publisher
    We employed a grp170-secreting tumour cell system to determine whether tumour cells engineered to secrete grp170 generate an antitumour-specific immune response...

More Information


  1. Rikke B, Yerg J, Battaglia M, Nagy T, Allison D, Johnson T. Quantitative trait Loci specifying the response of body temperature to dietary restriction. J Gerontol A Biol Sci Med Sci. 2004;59:118-25 pubmed
    ..Importantly, the genes underlying such QTLs would be causal factors affecting these responses and could be identified by positional cloning. ..
  2. Takizawa S, Izuhara Y, Kitao Y, Hori O, Ogawa S, Morita Y, et al. A novel inhibitor of advanced glycation and endoplasmic reticulum stress reduces infarct volume in rat focal cerebral ischemia. Brain Res. 2007;1183:124-37 pubmed
    ..In conclusion, TM2002 significantly ameliorates ischemic cerebral damage through reduction of ER stress, advanced glycation, and oxidative stress, independently of blood pressure lowering. ..
  3. Saba L, Bhave S, Grahame N, Bice P, Lapadat R, Belknap J, et al. Candidate genes and their regulatory elements: alcohol preference and tolerance. Mamm Genome. 2006;17:669-88 pubmed
  4. Gao P, Sun X, Chen X, Subjeck J, Wang X. Secretion of stress protein grp170 promotes immune-mediated inhibition of murine prostate tumor. Cancer Immunol Immunother. 2009;58:1319-28 pubmed publisher
    ..tumor cells (TRAMP-C2) by stable transfection with a secretable form of endoplasmic reticulum resident chaperone grp170 significantly enhances its immunogenicity in vivo...
  5. Zhao L, Rosales C, Seburn K, Ron D, Ackerman S. Alteration of the unfolded protein response modifies neurodegeneration in a mouse model of Marinesco-Sjögren syndrome. Hum Mol Genet. 2010;19:25-35 pubmed publisher
    ..Here, we report that overexpression of HYOU1/ORP150, an exchange factor that works in parallel to SIL1, prevents ER stress and rescues neurodegeneration in Sil1(-/-) ..
  6. Zuo D, Yu X, Guo C, Yi H, Chen X, Conrad D, et al. Molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity. FASEB J. 2012;26:1493-505 pubmed publisher
    ..We found that glucose-regulated protein 170 (Grp170), the largest stress protein and molecular chaperone, is highly efficient in binding CpG oligodeoxynucleotides (..
  7. Wang H, Yu X, Guo C, Zuo D, Fisher P, Subjeck J, et al. Enhanced endoplasmic reticulum entry of tumor antigen is crucial for cross-presentation induced by dendritic cell-targeted vaccination. J Immunol. 2013;191:6010-21 pubmed publisher
    ..Directing the clinically relevant, melanoma Ag gp100 to mouse-derived DCs by molecular adjuvant and chaperone Grp170 substantially facilitates Ag access to the ER...
  8. Montesi M, Jahn K, Bonewald L, Stea S, Bordini B, Beraudi A. Hypoxia mediates osteocyte ORP150 expression and cell death in vitro. Mol Med Rep. 2016;14:4248-4254 pubmed publisher
    ..Oxygen?regulated protein 150 (ORP150) is an endoplasmic reticulum?associated chaperone that has been observed to serve an important role in the ..
  9. Wang X, Arnouk H, Chen X, Kazim L, Repasky E, Subjeck J. Extracellular targeting of endoplasmic reticulum chaperone glucose-regulated protein 170 enhances tumor immunity to a poorly immunogenic melanoma. J Immunol. 2006;177:1543-51 pubmed
    ..that immunization with tumor-derived endoplasmic reticulum (ER) chaperone glucose-regulated protein 170 (grp170) elicits potent antitumor immunity...
  10. Park J, Easton D, Chen X, MacDonald I, Wang X, Subjeck J. The chaperoning properties of mouse grp170, a member of the third family of hsp70 related proteins. Biochemistry. 2003;42:14893-902 pubmed
    The 170 kDa glucose-regulated protein (grp170) is an endoplasmic reticulum resident protein that shares some sequence homology with both the hsp70 and hsp110 heat shock protein (hsp) families, yet is representative of a third and unique ..
  11. Matsushita K, Matsuyama T, Nishimura H, Takaoka T, Kuwabara K, Tsukamoto Y, et al. Marked, sustained expression of a novel 150-kDa oxygen-regulated stress protein, in severely ischemic mouse neurons. Brain Res Mol Brain Res. 1998;60:98-106 pubmed
    The 150-kDa oxygen-regulated protein (ORP150) first was described with reference to the central nervous system in cultured astrocytes subjected to dense hypoxia...
  12. Kitao Y, Imai Y, Ozawa K, Kataoka A, Ikeda T, Soda M, et al. Pael receptor induces death of dopaminergic neurons in the substantia nigra via endoplasmic reticulum stress and dopamine toxicity, which is enhanced under condition of parkin inactivation. Hum Mol Genet. 2007;16:50-60 pubmed
    ..by their decreased survival in mice deficient in the ubiquitin-protein ligase Parkin and the ER chaperone ORP150 (150 kDa oxygen-regulated protein)...
  13. Kitao Y, Matsuyama T, Takano K, Tabata Y, Yoshimoto T, Momoi T, et al. Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity?. Antioxid Redox Signal. 2007;9:589-95 pubmed
    ..What is the significance of ORP150/HSP12A, a molecular chaperone in the endoplasmic reticulum (ER), in the nigrostriatal system? Dopaminergic ..
  14. Kobayashi T, Iguchi T, Ohta Y. Abetalipoproteinemia induced by overexpression of ORP150 in mice. Comp Med. 2007;57:247-54 pubmed
    b>ORP150 is an endoplasmic-resident, hypoxic stress-induced protein, but little is known about the effects of its systemic overexpression. We have produced a transgenic strain of mice that overexpress ORP150 (ORP-Tg mice)...
  15. Ozawa K, Miyazaki M, Matsuhisa M, Takano K, Nakatani Y, Hatazaki M, et al. The endoplasmic reticulum chaperone improves insulin resistance in type 2 diabetes. Diabetes. 2005;54:657-63 pubmed
    ..mated with either heterozygous knockout mice or two types of transgenic mice of 150-kDa oxygen-regulated protein (ORP150), a molecular chaperone located in the ER...
  16. Kitano H, Nishimura H, Tachibana H, Yoshikawa H, Matsuyama T. ORP150 ameliorates ischemia/reperfusion injury from middle cerebral artery occlusion in mouse brain. Brain Res. 2004;1015:122-8 pubmed
    The 150-kDa oxygen-regulated protein (ORP150), a novel stress protein localized to the endoplasmic reticulum (ER), is induced by hypoxia/ischemia...
  17. Meunier L, Usherwood Y, Chung K, Hendershot L. A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins. Mol Biol Cell. 2002;13:4456-69 pubmed
    ..CaBP1; protein disulfide isomerase (PDI); ERdj3, a recently identified ER Hsp40 cochaperone; cyclophilin B; ERp72; GRP170; UDP-glucosyltransferase; and SDF2-L1...
  18. Kameya S, Hawes N, Chang B, Heckenlively J, Naggert J, Nishina P. Mfrp, a gene encoding a frizzled related protein, is mutated in the mouse retinal degeneration 6. Hum Mol Genet. 2002;11:1879-86 pubmed
    ..We also observed the localization of Wnt family proteins in the apical membrane of the RPE. Our results provide genetic evidence for an involvement of the Mfrp gene expressed by RPE in the degeneration of photoreceptors. ..
  19. Tanaka K, Shirai A, Ito Y, Namba T, Tahara K, Yamakawa N, et al. Expression of 150-kDa oxygen-regulated protein (ORP150) stimulates bleomycin-induced pulmonary fibrosis and dysfunction in mice. Biochem Biophys Res Commun. 2012;425:818-24 pubmed publisher
    ..One of ER chaperones, the 150-kDa oxygen-regulated protein (ORP150), is essential for the maintenance of cellular viability under stress conditions...
  20. Naved A, Ozawa M, Yu S, Miyauchi T, Muramatsu H, Muramatsu T. CBP-140, a novel endoplasmic reticulum resident Ca(2+)-binding protein with a carboxy-terminal NDEL sequence showed partial homology with 70-kDa heat shock protein (hsp70). Cell Struct Funct. 1995;20:133-41 pubmed
  21. Park J, Facciponte J, Chen X, Macdonald I, Repasky E, Manjili M, et al. Chaperoning function of stress protein grp170, a member of the hsp70 superfamily, is responsible for its immunoadjuvant activity. Cancer Res. 2006;66:1161-8 pubmed
    ..For members of the hsp70 superfamily, like grp170, this seems to be due to (a) the chaperoning of antigenic peptide by the stress protein and (b) the binding of the ..
  22. Bando Y, Tsukamoto Y, Katayama T, Ozawa K, Kitao Y, Hori O, et al. ORP150/HSP12A protects renal tubular epithelium from ischemia-induced cell death. FASEB J. 2004;18:1401-3 pubmed
    The 150 kDa oxygen-regulated protein (ORP150) is an inducible endoplasmic reticulum (ER) chaperone with cytoprotective properties in settings of cell stress, such as ischemia/reperfusion (I/R)...
  23. Dudek J, Volkmer J, Bies C, Guth S, Müller A, Lerner M, et al. A novel type of co-chaperone mediates transmembrane recruitment of DnaK-like chaperones to ribosomes. EMBO J. 2002;21:2958-67 pubmed
  24. Ikeda J, Kaneda S, Kuwabara K, Ogawa S, Kobayashi T, Matsumoto M, et al. Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150. Biochem Biophys Res Commun. 1997;230:94-9 pubmed
    We have cloned a cDNA encoding the human 150 kDa oxygen-regulated protein (ORP150) from hypoxia-treated astrocytoma U373 cDNA library...