Gene Symbol: Hsf2
Description: heat shock factor 2
Alias: AI661205, heat shock factor protein 2, HSF 2, HSTF 2, heat shock transcription factor 2
Species: mouse
Products:     Hsf2

Top Publications

  1. McMillan D, Christians E, Forster M, Xiao X, Connell P, Plumier J, et al. Heat shock transcription factor 2 is not essential for embryonic development, fertility, or adult cognitive and psychomotor function in mice. Mol Cell Biol. 2002;22:8005-14 pubmed
    ..In mammals, HSF1 is the main factor controlling the stress-inducible expression of Hsp genes while the functions of HSF2 and HSF4 are less clear...
  2. Akerfelt M, Henriksson E, Laiho A, Vihervaara A, Rautoma K, Kotaja N, et al. Promoter ChIP-chip analysis in mouse testis reveals Y chromosome occupancy by HSF2. Proc Natl Acad Sci U S A. 2008;105:11224-9 pubmed publisher
    ..promoter microarray (ChIP-chip) on mouse testis, we found a striking in vivo MSYq occupancy by heat shock factor 2 (HSF2), a transcription factor involved in spermatogenesis...
  3. Chang Y, Östling P, Akerfelt M, Trouillet D, Rallu M, Gitton Y, et al. Role of heat-shock factor 2 in cerebral cortex formation and as a regulator of p35 expression. Genes Dev. 2006;20:836-47 pubmed
    ..b>Hsf2-null mice display gametogenesis defects and brain abnormalities characterized by enlarged ventricles...
  4. Sarge K, Zimarino V, Holm K, Wu C, Morimoto R. Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability. Genes Dev. 1991;5:1902-11 pubmed
    ..Both mHSF1 and mHSF2 are encoded by single-copy genes, and neither is transcriptionally regulated by heat shock. However, there is a striking difference in the levels of mHSF1 mRNA in different tissues of the mouse. ..
  5. Fujimoto M, Izu H, Seki K, Fukuda K, Nishida T, Yamada S, et al. HSF4 is required for normal cell growth and differentiation during mouse lens development. EMBO J. 2004;23:4297-306 pubmed
    ..HSF1 and HSF2 are required for some developmental processes, but it is unclear how they regulate these processes...
  6. Wang G, Ying Z, Jin X, Tu N, Zhang Y, Phillips M, et al. Essential requirement for both hsf1 and hsf2 transcriptional activity in spermatogenesis and male fertility. Genesis. 2004;38:66-80 pubmed
    ..In contrast to hsf1(-/-) mice, which exhibit normal spermatogenesis, targeted disruption of hsf2 results in reduced testicular size but only a small impairment in male fertility...
  7. Björk J, Sandqvist A, Elsing A, Kotaja N, Sistonen L. miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis. Development. 2010;137:3177-84 pubmed publisher
    ..Here, we describe a novel function for miR-18 in mouse. We show that miR-18 directly targets heat shock factor 2 (HSF2), a transcription factor that influences a wide range of developmental processes including embryogenesis and ..
  8. Paslaru L, Morange M, Mezger V. Phenotypic characterization of mouse embryonic fibroblasts lacking heat shock factor 2. J Cell Mol Med. 2003;7:425-35 pubmed
    ..b>HSF2 has been shown to be involved in meiosis and mouse brain development...
  9. Wang G, Zhang J, Moskophidis D, Mivechi N. Targeted disruption of the heat shock transcription factor (hsf)-2 gene results in increased embryonic lethality, neuronal defects, and reduced spermatogenesis. Genesis. 2003;36:48-61 pubmed
    ..Hsf1 is recognized as the most effective transactivator of Hsps in response to thermal challenge, but the role of Hsf2 in regulation of genes under normal or increased stress conditions in vivo remains elusive...

More Information


  1. Manuel M, Rallu M, Loones M, Zimarino V, Mezger V, Morange M. Determination of the consensus binding sequence for the purified embryonic heat shock factor 2. Eur J Biochem. 2002;269:2527-37 pubmed
    ..HSE are composed of inverted repeats of the pentamer nGAAm. In this study, we compare the embryonic HSF2 protein, purified from F9 embryonal carcinoma cells tumor, and the in vitro synthesized HSF2...
  2. Le Masson F, Christians E. HSFs and regulation of Hsp70.1 (Hspa1b) in oocytes and preimplantation embryos: new insights brought by transgenic and knockout mouse models. Cell Stress Chaperones. 2011;16:275-85 pubmed publisher
    ..Finally, submitting blastocyst to different stress conditions, we show that HSF2 is differentially required for Hsp expression and cell survival...
  3. Le Masson F, Razak Z, Kaigo M, Audouard C, Charry C, Cooke H, et al. Identification of heat shock factor 1 molecular and cellular targets during embryonic and adult female meiosis. Mol Cell Biol. 2011;31:3410-23 pubmed publisher
    ..This work provides new insights into meiotic gene regulation and points out potential links between cellular stress and the meiotic anomalies frequently observed in humans. ..
  4. Shinkawa T, Tan K, Fujimoto M, Hayashida N, Yamamoto K, Takaki E, et al. Heat shock factor 2 is required for maintaining proteostasis against febrile-range thermal stress and polyglutamine aggregation. Mol Biol Cell. 2011;22:3571-83 pubmed publisher
    ..HSF1 is a master regulator of HSP expression during heat shock in mammals, as is HSF3 in avians. HSF2 plays roles in development of the brain and reproductive organs...
  5. Rallu M, Loones M, Lallemand Y, Morimoto R, Morange M, Mezger V. Function and regulation of heat shock factor 2 during mouse embryogenesis. Proc Natl Acad Sci U S A. 1997;94:2392-7 pubmed
    ..In particular, the mouse HSF2 has been found to be active in testis and during preimplantation development...
  6. Goodson M, Park Sarge O, Sarge K. Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activities. Mol Cell Biol. 1995;15:5288-93 pubmed
    Heat shock factor 2 (HSF2) functions as a transcriptional regulator of heat shock protein gene expression in mammalian cells undergoing processes of differentiation and development...
  7. Kallio M, Chang Y, Manuel M, Alastalo T, Rallu M, Gitton Y, et al. Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice. EMBO J. 2002;21:2591-601 pubmed
    ..By disrupting the Hsf2 gene, we show that, although the lack of HSF2 is not embryonic lethal, Hsf2(-/-) mice suffer from brain ..
  8. Lecomte S, Desmots F, Le Masson F, Le Goff P, Michel D, Christians E, et al. Roles of heat shock factor 1 and 2 in response to proteasome inhibition: consequence on p53 stability. Oncogene. 2010;29:4216-24 pubmed publisher
    ..of the specific or redundant functions of the different members of the HSF family and in particular of HSF1 and HSF2, which are both ubiquitously expressed...
  9. Fujimoto M, Oshima K, Shinkawa T, Wang B, Inouye S, Hayashida N, et al. Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses. J Biol Chem. 2008;283:29961-70 pubmed publisher
    ..genes in vivo in mouse lenses, and a substantial numbers of the regions were also occupied by HSF1 and HSF2. HSF4 regulated expression of some genes at a developmental stage when HSF1 and HSF2 expression decreased...
  10. Korfanty J, Stokowy T, Widlak P, Gogler Piglowska A, Handschuh L, Podkowiński J, et al. Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes. Int J Biochem Cell Biol. 2014;57:76-83 pubmed publisher
    ..Heat Shock Factor 1 (HSF1) is the primary transcription factor responsible for the response to cellular stress, while HSF2 becomes activated during development and differentiation, including spermatogenesis...
  11. Revel F, Renard J, Duranthon V. PCR-generated cDNA libraries from reduced numbers of mouse oocytes. Zygote. 1995;3:241-50 pubmed
    ..008% of the mRNA. The repeatable generation of representative cDNA libraries from reduced numbers of oocytes or embryos should open new opportunities for obtaining genetic information from mammalian preimplantation embryos. ..
  12. Yang X, Dale E, Diaz J, Shyamala G. Estrogen dependent expression of heat shock transcription factor: implications for uterine synthesis of heat shock proteins. J Steroid Biochem Mol Biol. 1995;52:415-9 pubmed
    ..This is the first demonstration of a cellular factor which can regulate HSF-1 and HSF-2 gene expression. The implications of these findings to uterine heat shock protein gene expression are discussed. ..
  13. Homma S, Jin X, Wang G, Tu N, Min J, Yanasak N, et al. Demyelination, astrogliosis, and accumulation of ubiquitinated proteins, hallmarks of CNS disease in hsf1-deficient mice. J Neurosci. 2007;27:7974-86 pubmed
    ..Mammalian cells express three hsfs (hsf1, hsf2, hsf4) and their role in CNS homeostasis remains unclear...
  14. Lecomte S, Reverdy L, Le Quement C, Le Masson F, Amon A, Le Goff P, et al. Unraveling complex interplay between heat shock factor 1 and 2 splicing isoforms. PLoS ONE. 2013;8:e56085 pubmed publisher
    ..The two main factors in this family, HSF1 and HSF2, are co-expressed in numerous tissues where they can interact and form heterotrimers in response to proteasome ..
  15. Akerfelt M, Vihervaara A, Laiho A, Conter A, Christians E, Sistonen L, et al. Heat shock transcription factor 1 localizes to sex chromatin during meiotic repression. J Biol Chem. 2010;285:34469-76 pubmed publisher
    ..Disruption of Hsf1, together with another HSF family member, Hsf2, causes male sterility and complete lack of mature sperm in mice, but the specific role of HSF1 in spermatogenesis ..
  16. Hayashida N. Set1/MLL complex is indispensable for the transcriptional ability of heat shock transcription factor 2. Biochem Biophys Res Commun. 2015;467:805-12 pubmed publisher
    b>Heat shock transcription factor 2 (HSF2) is one of four mammalian HSFs, and it is essential in neurogenesis and gametogenesis. However, other aspects of this transcription factor have not been thoroughly characterized...
  17. Kajiya H, Ito M, Ohshima H, Kenmotsu S, Ries W, Benjamin I, et al. RANK ligand expression in heat shock factor-2 deficient mouse bone marrow stromal/preosteoblast cells. J Cell Biochem. 2006;97:1362-9 pubmed
    ..Novel therapeutic agents that modulate HSF-2 activation may have therapeutic utility against increased levels of FGF-2 and bone destruction associated with pathologic conditions. ..
  18. Murphy L, Wilkerson D, Hong Y, Sarge K. PRC1 associates with the hsp70i promoter and interacts with HSF2 during mitosis. Exp Cell Res. 2008;314:2224-30 pubmed publisher
    ..b>HSF2 is a DNA binding protein that we previously showed bookmarks the hsp70i gene during mitosis, an epigenetic ..
  19. Wong R, Wlodarczyk B, Min K, Scott M, Kartiko S, Yu W, et al. Mouse Fkbp8 activity is required to inhibit cell death and establish dorso-ventral patterning in the posterior neural tube. Hum Mol Genet. 2008;17:587-601 pubmed
    ..The mutant Fkbp8 allele is a new experimental model which will be useful in dissecting the pathogenesis of spinal NTDs, and enhance our understanding of the etiology of human NTDs...
  20. Fiorenza M, Bevilacqua A, Canterini S, Torcia S, Pontecorvi M, Mangia F. Early transcriptional activation of the hsp70.1 gene by osmotic stress in one-cell embryos of the mouse. Biol Reprod. 2004;70:1606-13 pubmed
    ..containing HSE, GAGA box or GC box consensus sequences, and antibodies raised to transcription factors HSF1, HSF2, Drosophila melanogaster GAGA factor, or Sp1 demonstrated that hsp70...
  21. Pirkkala L, Nykanen P, Sistonen L. Roles of the heat shock transcription factors in regulation of the heat shock response and beyond. FASEB J. 2001;15:1118-31 pubmed
    ..HSF1, an HSF prototype, and HSF3 are responsible for heat-induced Hsp expression, whereas HSF2 is refractory to classical stressors...
  22. Eriksson M, Jokinen E, Sistonen L, Leppa S. Heat shock factor 2 is activated during mouse heart development. Int J Dev Biol. 2000;44:471-7 pubmed
    Two members of the heat shock transcription factor family, HSF1 and HSF2, have been identified as activators of mammalian heat shock gene expression...
  23. Hong Y, Sarge K. Regulation of protein phosphatase 2A activity by heat shock transcription factor 2. J Biol Chem. 1999;274:12967-70 pubmed
    ..Here, it is shown that a member of the HSF family, HSF2, interacts with the PR65 (A) subunit of protein phosphatase 2A (PP2A)...
  24. Wilkerson D, Murphy L, Sarge K. Interaction of HSF1 and HSF2 with the Hspa1b promoter in mouse epididymal spermatozoa. Biol Reprod. 2008;79:283-8 pubmed publisher
    ..In this report, we investigated the role of HSF1 and HSF2 in bookmarking Hspa1b during late spermatogenesis...
  25. Batulan Z, Shinder G, Minotti S, He B, Doroudchi M, Nalbantoglu J, et al. High threshold for induction of the stress response in motor neurons is associated with failure to activate HSF1. J Neurosci. 2003;23:5789-98 pubmed
    ..when proteasome activity was sufficiently inhibited to induce accumulation of an alternative transcription factor HSF2. These results indicate that the high threshold for induction of the stress response in motor neurons stems from an ..
  26. Min J, Han M, Lee S, Kim K, Park Y. Regulation of rat heat shock factor 2 expression during the early organogenic phase of embryogenesis. Biochim Biophys Acta. 2000;1494:256-62 pubmed
    ..In vertebrates, HSF2 has been suggested to mediate the transcriptional regulation of hsp gene expression during development and ..
  27. Manuel M, Sage J, Mattei M, Morange M, Mezger V. Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences. Gene. 1999;232:115-24 pubmed
    The mouse heat shock factor 2 (HSF2) cDNA was previously cloned by homology to HSF1, the heat shock factor involved in the cellular response to stress [Sarge, K.D., Zimarino, V., Holm, K., Wu, C., Morimoto, R.I...
  28. El Fatimy R, Miozzo F, Le Mouël A, Abane R, Schwendimann L, Sabéran Djoneidi D, et al. Heat shock factor 2 is a stress-responsive mediator of neuronal migration defects in models of fetal alcohol syndrome. EMBO Mol Med. 2014;6:1043-61 pubmed publisher
    ..We used normal and Hsf2-deficient mice and cell systems to uncover a pivotal role for heat shock factor 2 (HSF2) in radial neuronal ..
  29. Elsing A, Aspelin C, Björk J, Bergman H, Himanen S, Kallio M, et al. Expression of HSF2 decreases in mitosis to enable stress-inducible transcription and cell survival. J Cell Biol. 2014;206:735-49 pubmed publisher
    ..Hsps are transcriptionally regulated by heat shock factors (HSFs). Of these, HSF1 is the master regulator and HSF2 modulates Hsp expression by interacting with HSF1...
  30. Park S, Kim S, Ahn S. HSF2 autoregulates its own transcription. Int J Mol Med. 2015;36:1173-9 pubmed publisher
    Heat shock factor 2 (HSF2) is one of the most important regulators affecting stress mechanisms, and is frequently amplified in the ubiquitin proteasome pathway...
  31. Sarge K, Park Sarge O, Kirby J, Mayo K, Morimoto R. Expression of heat shock factor 2 in mouse testis: potential role as a regulator of heat-shock protein gene expression during spermatogenesis. Biol Reprod. 1994;50:1334-43 pubmed
    We have examined the expression and function of heat shock transcription factor 2 (HSF2) in spermatogenic cells of mouse testis...
  32. Borowiec A, Sion B, Chalmel F, D Rolland A, Lemonnier L, De Clerck T, et al. Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation. FASEB J. 2016;30:3155-70 pubmed publisher
    ..Dewailly, E., Slomianny, C., Mauduit, C., Benahmed, M., Roudbaraki, M., Jégou, B., Prevarskaya, N., Bidaux, G. Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation. ..