Hoxb8

Summary

Gene Symbol: Hoxb8
Description: homeobox B8
Alias: Hox-2.4, homeobox protein Hox-B8, homeo box B8, homeobox protein Hox-2.4
Species: mouse
Products:     Hoxb8

Top Publications

  1. Chen F, Capecchi M. Targeted mutations in hoxa-9 and hoxb-9 reveal synergistic interactions. Dev Biol. 1997;181:186-96 pubmed
    ..In particular, the sternum defects are greatly exacerbated. Interestingly, the defects in hoxb-9 and hoxa-9/ hoxb-9 mutant mice are concentrated along the axial column at points of transition between vertebral types. ..
  2. Knoepfler P, Sykes D, Pasillas M, Kamps M. HoxB8 requires its Pbx-interaction motif to block differentiation of primary myeloid progenitors and of most cell line models of myeloid differentiation. Oncogene. 2001;20:5440-8 pubmed
    b>HoxB8 was the first homeobox gene identified as a cause of leukemia. In murine WEHI3B acute myeloid leukemia (AML) cells, proviral integration leads to the expression of both HoxB8 and Interleukin (IL-3)...
  3. Medina Martinez O, Ramirez Solis R. In vivo mutagenesis of the Hoxb8 hexapeptide domain leads to dominant homeotic transformations that mimic the loss-of-function mutations in genes of the Hoxb cluster. Dev Biol. 2003;264:77-90 pubmed
    ..To study the significance of these interactions in vivo, we have generated mice that carry mutations in the Hoxb8 hexapeptide motif...
  4. Li X, Isono K, Yamada D, Endo T, Endoh M, Shinga J, et al. Mammalian polycomb-like Pcl2/Mtf2 is a novel regulatory component of PRC2 that can differentially modulate polycomb activity both at the Hox gene cluster and at Cdkn2a genes. Mol Cell Biol. 2011;31:351-64 pubmed publisher
    ..We therefore propose a novel role for Pcl2 to modify functional engagement of PRC2 and PRC1, which could be modulated by sensing cellular circumstances. ..
  5. Perkins A, Cory S. Conditional immortalization of mouse myelomonocytic, megakaryocytic and mast cell progenitors by the Hox-2.4 homeobox gene. EMBO J. 1993;12:3835-46 pubmed
    ..Thus ectopic expression of Hox-2.4 enhances self-renewal of immature myeloid progenitors and progression to a fully malignant state is favoured by somatic mutations conferring autocrine production of IL-3. ..
  6. Suzuki M, Mizutani Koseki Y, Fujimura Y, Miyagishima H, Kaneko T, Takada Y, et al. Involvement of the Polycomb-group gene Ring1B in the specification of the anterior-posterior axis in mice. Development. 2002;129:4171-83 pubmed
    ..homeotic transformations of the axial skeleton and a mild derepression of some Hox genes (Hoxb4, Hoxb6 and Hoxb8) in cells anterior to their normal boundaries of expression in the mesodermal compartment...
  7. Agarwal P, Wylie J, Galceran J, Arkhitko O, Li C, Deng C, et al. Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo. Development. 2003;130:623-33 pubmed
    ..These data suggest common pathways for the differentiation and growth of embryonic structures downstream of T-box genes. ..
  8. Capellini T, Zewdu R, Di Giacomo G, Asciutti S, Kugler J, Di Gregorio A, et al. Pbx1/Pbx2 govern axial skeletal development by controlling Polycomb and Hox in mesoderm and Pax1/Pax9 in sclerotome. Dev Biol. 2008;321:500-14 pubmed publisher
  9. Bittner D, De Robertis E, Cho K. Characterization of the Xenopus Hox 2.4 gene and identification of control elements in its intron. Dev Dyn. 1993;196:11-24 pubmed
    ..The short distance between the end of the Hox 2.4 cDNA and the start site of the neighboring gene in the complex raises the possibility that this transcriptional element might be shared by two Hox genes. ..

More Information

Publications62

  1. Bogue C, Gross I, Vasavada H, Dynia D, Wilson C, Jacobs H. Identification of Hox genes in newborn lung and effects of gestational age and retinoic acid on their expression. Am J Physiol. 1994;266:L448-54 pubmed
    ..We conclude that there are many homeobox genes expressed in developing rodent lung and that their mRNA levels are affected by both gestational age and RA...
  2. Juan A, Ruddle F. Enhancer timing of Hox gene expression: deletion of the endogenous Hoxc8 early enhancer. Development. 2003;130:4823-34 pubmed
    ..Last, our data is consistent with the position that disparate regulatory pathways are responsible for the expression of Hoxc8 in the organogenesis of somites, neural tube and limb bud. ..
  3. Oosterveen T, Meijlink F, Deschamps J. Expression of retinaldehyde dehydrogenase II and sequential activation of 5' Hoxb genes in the mouse caudal hindbrain. Gene Expr Patterns. 2004;4:243-7 pubmed
    ..5 and E11.5 in the caudal hindbrain, and that this differential expansion occurs just rostrally to the localisation of the transcripts for the RA biosynthetic enzyme Raldh2 in the cervical mesenchyme. ..
  4. Mansfield J, Harfe B, Nissen R, Obenauer J, Srineel J, Chaudhuri A, et al. MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat Genet. 2004;36:1079-83 pubmed
    ..Furthermore, miR-196a negatively regulates Hoxb8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox ..
  5. Isono K, Fujimura Y, Shinga J, Yamaki M, O Wang J, Takihara Y, et al. Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes. Mol Cell Biol. 2005;25:6694-706 pubmed
    ..Functional redundancy between Phc2 and Phc1 leads us to hypothesize that the overall level of polyhomeotic orthologues in nuclei is a parameter that is critical in enabling the class II PcG complexes to exert their molecular functions. ..
  6. Ahmari S. Using mice to model Obsessive Compulsive Disorder: From genes to circuits. Neuroscience. 2016;321:121-137 pubmed publisher
  7. Hunt P, Gulisano M, Cook M, Sham M, Faiella A, Wilkinson D, et al. A distinct Hox code for the branchial region of the vertebrate head. Nature. 1991;353:861-4 pubmed
  8. Qian Y, Shirasawa S, Chen C, Cheng L, Ma Q. Proper development of relay somatic sensory neurons and D2/D4 interneurons requires homeobox genes Rnx/Tlx-3 and Tlx-1. Genes Dev. 2002;16:1220-33 pubmed
    ..Together, our studies demonstrate a central role for Rnx and Tlx-1 in the development of two major classes of relay sensory neurons, somatic and visceral. ..
  9. Greer J, Capecchi M. Hoxb8 is required for normal grooming behavior in mice. Neuron. 2002;33:23-34 pubmed
    ..Genes that influence this complex behavior are unknown. We report that mice with disruptions of Hoxb8 show, with 100% penetrance, excessive grooming leading to hair removal and lesions...
  10. Fujino T, Yamazaki Y, Largaespada D, Jenkins N, Copeland N, Hirokawa K, et al. Inhibition of myeloid differentiation by Hoxa9, Hoxb8, and Meis homeobox genes. Exp Hematol. 2001;29:856-63 pubmed
    The homeobox gene Hoxb8 is activated in the murine myelomonocytic cell line WEHI-3B as a result of intracisternal A particle integration...
  11. van den Akker E, Fromental Ramain C, de Graaff W, Le Mouellic H, Brulet P, Chambon P, et al. Axial skeletal patterning in mice lacking all paralogous group 8 Hox genes. Development. 2001;128:1911-21 pubmed
    ..The phenotype of Hoxd8 loss-of-function mutants is presented, and compared with that of Hoxb8- and Hoxc8-null mice...
  12. Zuniga A, Zeller R. Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity. Development. 1999;126:13-21 pubmed
    ..Concurrently, Gli3 functions independently of formin during initial posterior nesting of 5'HoxD domains, whereas their subsequent distal restriction and anterior expansion depends on genetic interaction of Gli3 and formin. ..
  13. Akasaka T, Kanno M, Balling R, Mieza M, Taniguchi M, Koseki H. A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton. Development. 1996;122:1513-22 pubmed
  14. Huang M, Huang T, Xiang Y, Xie Z, Chen Y, Yan R, et al. Ptf1a, Lbx1 and Pax2 coordinate glycinergic and peptidergic transmitter phenotypes in dorsal spinal inhibitory neurons. Dev Biol. 2008;322:394-405 pubmed publisher
    ..Our studies therefore suggest a coordinated and hierarchical specification of a variety of neurotransmitters in dorsal spinal inhibitory neurons. ..
  15. Bogarad L, Utset M, Awgulewitsch A, Miki T, Hart C, Ruddle F. The developmental expression pattern of a new murine homeo box gene: Hox-2.5. Dev Biol. 1989;133:537-49 pubmed
  16. Hart C, Fainsod A, Ruddle F. Sequence analysis of the murine Hox-2.2, -2.3, and -2.4 homeo boxes: evolutionary and structural comparisons. Genomics. 1987;1:182-95 pubmed
  17. El Mounayri O, Triplett J, Yates C, Herring B. Regulation of smooth muscle-specific gene expression by homeodomain proteins, Hoxa10 and Hoxb8. J Biol Chem. 2005;280:25854-63 pubmed
    ..reverse transcription-PCR coupled to cDNA library screening we identified two homeodomain proteins, Hoxa10 and Hoxb8, which are expressed in adult mouse smooth muscle tissues...
  18. Yagi H, Deguchi K, Aono A, Tani Y, Kishimoto T, Komori T. Growth disturbance in fetal liver hematopoiesis of Mll-mutant mice. Blood. 1998;92:108-17 pubmed
    ..These data show that the hematopoietic precursors were greatly reduced in mutant mice, and suggest that Mll functions as a regulator of the growth of hematopoietic precursors. ..
  19. van den Akker E, Forlani S, Chawengsaksophak K, de Graaff W, Beck F, Meyer B, et al. Cdx1 and Cdx2 have overlapping functions in anteroposterior patterning and posterior axis elongation. Development. 2002;129:2181-93 pubmed
    ..The function of Cdx genes in morphogenetic processes during gastrulation and tail bud extension, and their relationship with the Hox genes are discussed in the light of available data in Amphioxus, C. elegans, Drosophila and mice. ..
  20. Young T, Rowland J, van de Ven C, Bialecka M, Novoa A, Carapuco M, et al. Cdx and Hox genes differentially regulate posterior axial growth in mammalian embryos. Dev Cell. 2009;17:516-26 pubmed publisher
    ..Concomitant regulation of Cyp26a1 expression, restraining retinoic acid signaling away from the posterior growth zone, may likewise play a role in timing the trunk-tail transition. ..
  21. Liao X, Collins M. All-trans retinoic acid-induced ectopic limb and caudal structures: murine strain sensitivities and pathogenesis. Dev Dyn. 2008;237:1553-64 pubmed publisher
    ..limbs were hindlimbs (expressing Pitx1 and Tbx4 but not Tbx5), yet they also expressed the predominantly forelimb Hoxb8. Ectopic body axis formation was indicated by gene expression for ectopic primitive streaks, notochords, and nodes,..
  22. Fujimura Y, Isono K, Vidal M, Endoh M, Kajita H, Mizutani Koseki Y, et al. Distinct roles of Polycomb group gene products in transcriptionally repressed and active domains of Hoxb8. Development. 2006;133:2371-81 pubmed
    ..Hox gene expression, we have focused on the binding patterns of PcG gene products to the flanking regions of the Hoxb8 gene in expressing and non-expressing tissues...
  23. Voss A, Collin C, Dixon M, Thomas T. Moz and retinoic acid coordinately regulate H3K9 acetylation, Hox gene expression, and segment identity. Dev Cell. 2009;17:674-86 pubmed publisher
    ..In conclusion, our data show that Moz regulates H3K9 acetylation at Hox gene loci and that RA can act independently of Moz to establish specific Hox gene expression boundaries. ..
  24. Charite J, de Graaff W, Shen S, Deschamps J. Ectopic expression of Hoxb-8 causes duplication of the ZPA in the forelimb and homeotic transformation of axial structures. Cell. 1994;78:589-601 pubmed
    ..Besides other alterations, posterior homeotic transformations of axial structures were observed, involving the first spinal (Froriep's) ganglion and several cervical vertebrae. ..
  25. Gee J, Smith N, Fernandez F, Economo M, Brunert D, Rothermel M, et al. Imaging activity in neurons and glia with a Polr2a-based and cre-dependent GCaMP5G-IRES-tdTomato reporter mouse. Neuron. 2014;83:1058-72 pubmed publisher
    ..Through electrophysiological and behavioral analyses, we determined that GCaMP5G expression had no major impact on nervous system performance. PC::G5-tdT will be instrumental for a variety of brain mapping experiments. ..
  26. Meijlink F, de Laaf R, Verrijzer P, Destree O, Kroezen V, Hilkens J, et al. A mouse homeobox containing gene on chromosome 11: sequence and tissue-specific expression. Nucleic Acids Res. 1987;15:6773-86 pubmed
    ..3; however, major quantitative differences in this response were observed. ..
  27. Carroll L, Capecchi M. Hoxc8 initiates an ectopic mammary program by regulating Fgf10 and Tbx3 expression and Wnt/β-catenin signaling. Development. 2015;142:4056-67 pubmed publisher
  28. Forlani S, Lawson K, Deschamps J. Acquisition of Hox codes during gastrulation and axial elongation in the mouse embryo. Development. 2003;130:3807-19 pubmed
  29. Rubock M, Larin Z, Cook M, Papalopulu N, Krumlauf R, Lehrach H. A yeast artificial chromosome containing the mouse homeobox cluster Hox-2. Proc Natl Acad Sci U S A. 1990;87:4751-5 pubmed
    ..The YAC will enable us to construct modified forms of the Hox-2 cluster in yeast and to identify their effect on the phenotype of the animal in transgenic mouse strains. ..
  30. Holstege J, de Graaff W, Hossaini M, Cardona Cano S, Jaarsma D, van den Akker E, et al. Loss of Hoxb8 alters spinal dorsal laminae and sensory responses in mice. Proc Natl Acad Sci U S A. 2008;105:6338-43 pubmed publisher
    ..Hoxb genes are expressed at high levels in the dorsal horn of the spinal cord. Hoxb8 null mutants manifest a striking phenotype of excessive grooming and hairless lesions on the lower back...
  31. Tiret L, Le Mouellic H, Lallemand Y, Maury M, Brulet P. Altering the spatial determinations in the mouse embryos by manipulating the Hox genes. C R Acad Sci III. 1993;316:1009-24 pubmed
    ..Expression of some related Hox genes in Hoxc-8 homozygous mutants is not altered, suggesting the absence of genetic interregulations between these genes. ..
  32. Blatt C, Aberdam D, Schwartz R, Sachs L. DNA rearrangement of a homeobox gene in myeloid leukaemic cells. EMBO J. 1988;7:4283-90 pubmed
    ..4 and that this resulted in constitutive expression of the homeobox gene. It is suggested that constitutive expression of the homeobox gene may interrupt the normal development program in these leukaemic cells. ..
  33. Ben David L, Aberdam D, Sachs L, Blatt C. A deletion and a rearrangement distinguish between the intracisternal A-particle of Hox-2.4 and that of interleukin-3 in the same leukemic cells. Virology. 1991;182:382-7 pubmed
    ..Both proviral insertions have resulted in transcriptional activation of the adjacent genes, which appears to be a significant step in the leukemogenic process in these leukemic cells. ..
  34. Jones F, Prediger E, Bittner D, De Robertis E, Edelman G. Cell adhesion molecules as targets for Hox genes: neural cell adhesion molecule promoter activity is modulated by cotransfection with Hox-2.5 and -2.4. Proc Natl Acad Sci U S A. 1992;89:2086-90 pubmed
    ..The results suggest that the N-CAM gene is likely to be a target for regulation by Hox gene products. ..
  35. van Rooijen C, Simmini S, Bialecka M, Neijts R, van de Ven C, Beck F, et al. Evolutionarily conserved requirement of Cdx for post-occipital tissue emergence. Development. 2012;139:2576-83 pubmed publisher
    ..Cdx requirement for the post-head section of the axis is ancestral as it takes place in arthropods as well. ..
  36. Ahn Y, Mullan H, Krumlauf R. Long-range regulation by shared retinoic acid response elements modulates dynamic expression of posterior Hoxb genes in CNS development. Dev Biol. 2014;388:134-44 pubmed publisher
    ..Our data demonstrate that multiple RAREs cooperate to remotely regulate 5? Hoxb genes during CNS development, providing a new insight into the mechanisms for gene regulation within the Hox clusters. ..
  37. Neijts R, Amin S, van Rooijen C, Deschamps J. Cdx is crucial for the timing mechanism driving colinear Hox activation and defines a trunk segment in the Hox cluster topology. Dev Biol. 2017;422:146-154 pubmed publisher
    ..Subsequently to initial Wnt-induced activation of 3' Hox genes, Cdx transcription factors would act as crucial effectors for activating central Hox genes, until the last gene of the cluster arrests the process. ..
  38. Deschamps J, van Nes J. Developmental regulation of the Hox genes during axial morphogenesis in the mouse. Development. 2005;132:2931-42 pubmed
    ..In particular, we highlight the integration of these transducers of anteroposterior positional information into the genetic network that drives tissue generation and patterning during axial elongation. ..
  39. Liu Y, Liu C, Yamada Y, Fan C. Growth arrest specific gene 1 acts as a region-specific mediator of the Fgf10/Fgf8 regulatory loop in the limb. Development. 2002;129:5289-300 pubmed
    ..Our data provide evidence that Gas1 acts to maintain high levels of FGF10 at the tip mesenchyme and support the proposal that Fgf10 expression in this region is crucial for maintaining Fgf8 expression in the AER. ..
  40. te Welscher P, Fernandez Teran M, Ros M, Zeller R. Mutual genetic antagonism involving GLI3 and dHAND prepatterns the vertebrate limb bud mesenchyme prior to SHH signaling. Genes Dev. 2002;16:421-6 pubmed
    ..These interactions polarize the nascent limb bud mesenchyme prior to SHH signaling. ..
  41. van den Akker E, Reijnen M, Korving J, Brouwer A, Meijlink F, Deschamps J. Targeted inactivation of Hoxb8 affects survival of a spinal ganglion and causes aberrant limb reflexes. Mech Dev. 1999;89:103-14 pubmed
    b>Hoxb8 mutant mice were generated by inserting the lacZ coding sequence in frame with the first exon of Hoxb8. These mice express a fusion protein with a functional beta-galactosidase activity instead of Hoxb8...
  42. Fanarraga M, Charite J, Hage W, de Graaff W, Deschamps J. Hoxb-8 gain-of-function transgenic mice exhibit alterations in the peripheral nervous system. J Neurosci Methods. 1997;71:11-8 pubmed
  43. Pollock R, Sreenath T, Ngo L, Bieberich C. Gain of function mutations for paralogous Hox genes: implications for the evolution of Hox gene function. Proc Natl Acad Sci U S A. 1995;92:4492-6 pubmed
    ..We propose that regional specialization of the vertebral column has been driven by regionalization of Hox gene function and that a major aspect of this evolutionary progression may have been restriction of Hox gene expression. ..
  44. Charite J, de Graaff W, Vogels R, Meijlink F, Deschamps J. Regulation of the Hoxb-8 gene: synergism between multimerized cis-acting elements increases responsiveness to positional information. Dev Biol. 1995;171:294-305 pubmed
    ..This factor is thus very likely to be involved in the transduction of positional signals. ..
  45. Zwartkruis F, Hoeijmakers T, Deschamps J, Meijlink F. The murine Hox-2.4 promoter contains a functional octamer motif. Nucleic Acids Res. 1992;20:1599-606 pubmed
    ..Cotransfection of Oct expression vectors influences the transcriptional activity of the promoter, suggesting that the Oct-gene family may be involved in regulating HOX genes. ..
  46. Isono K, Mizutani Koseki Y, Komori T, Schmidt Zachmann M, Koseki H. Mammalian polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1. Genes Dev. 2005;19:536-41 pubmed
    ..Importantly, the levels of Sf3b1 in PcG complexes were decreased in Sf3b1-heterozygous embryos. These findings suggest that Sf3b1-PcG protein interaction is essential for true PcG-mediated repression of Hox genes. ..
  47. Medina Martinez O, Bradley A, Ramirez Solis R. A large targeted deletion of Hoxb1-Hoxb9 produces a series of single-segment anterior homeotic transformations. Dev Biol. 2000;222:71-83 pubmed
    ..Thus, Hoxb13 expression seems to be independent of the deleted region, suggesting that its expression pattern could be achieved independent of the colinear pattern of the cluster or by a regulatory element located 5' of Hoxb9. ..
  48. Zimmermann F, Rich I. Mammalian homeobox B6 expression can be correlated with erythropoietin production sites and erythropoiesis during development, but not with hematopoietic or nonhematopoietic stem cell populations. Blood. 1997;89:2723-35 pubmed
    ..The results indicate that HOX B6 is intimately involved in the regulation of the erythropoietic system and could be a marker for this lineage. ..
  49. Szabo N, Da Silva R, Sotocinal S, Zeilhofer H, Mogil J, Kania A. Hoxb8 intersection defines a role for Lmx1b in excitatory dorsal horn neuron development, spinofugal connectivity, and nociception. J Neurosci. 2015;35:5233-46 pubmed publisher
    ..In this study, we use the intersection of Lmx1b and Hoxb8::Cre expression in the spinal cord to genetically define nociceptive circuits...
  50. Wilkinson D, Bhatt S, Cook M, Boncinelli E, Krumlauf R. Segmental expression of Hox-2 homoeobox-containing genes in the developing mouse hindbrain. Nature. 1989;341:405-9 pubmed
    ..These data indicate that Hox genes specify segment phenotype, a role analogous to that of their Drosophila homologues. ..
  51. Graham A, Papalopulu N, Krumlauf R. The murine and Drosophila homeobox gene complexes have common features of organization and expression. Cell. 1989;57:367-78 pubmed
    ..Our results argue that these complexes arose from a common ancestor, present before the divergence of lineages that gave rise to arthropods and vertebrates. ..
  52. Wong S, Agarwal V, Mansfield J, Denans N, Schwartz M, Prosser H, et al. Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs. Proc Natl Acad Sci U S A. 2015;112:E4884-93 pubmed publisher
    ..By feeding into, and thereby integrating, multiple genetic networks controlling vertebral number and identity, miR-196 is a critical player defining axial formulae. ..
  53. Zach F, Mueller A, Gessner A. Production and Functional Characterization of Murine Osteoclasts Differentiated from ER-Hoxb8-Immortalized Myeloid Progenitor Cells. PLoS ONE. 2015;10:e0142211 pubmed publisher
    ..the present study, we therefore established a novel protocol for the differentiation of osteoclasts from murine ER-Hoxb8-immortalized myeloid stem cells...