Gene Symbol: Hhex
Description: hematopoietically expressed homeobox
Alias: Hex, Hex1, Hhex-rs2, Prh, Prhx, hematopoietically-expressed homeobox protein Hhex, hematopoietically expressed homeobox, related sequence 2, homeobox protein HEX, homeobox protein PRH, mHex
Species: mouse
Products:     Hhex

Top Publications

  1. Wilson N, Foster S, Wang X, Knezevic K, Schütte J, Kaimakis P, et al. Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide analysis of ten major transcriptional regulators. Cell Stem Cell. 2010;7:532-44 pubmed publisher
    ..Taken together, this study demonstrates the power of genome-wide analysis in generating novel functional insights into the transcriptional control of stem and progenitor cells. ..
  2. Warr N, Powles Glover N, Chappell A, Robson J, Norris D, Arkell R. Zic2-associated holoprosencephaly is caused by a transient defect in the organizer region during gastrulation. Hum Mol Genet. 2008;17:2986-96 pubmed publisher
    ..The analysis provides genetic evidence that Zic2 functions during organizer formation and that the PCP develops via a multi-step process. ..
  3. Beck S, Le Good J, Guzman M, Ben Haim N, Roy K, Beermann F, et al. Extraembryonic proteases regulate Nodal signalling during gastrulation. Nat Cell Biol. 2002;4:981-5 pubmed
    ..A lack of Spc1 and Spc4 affects both pathways because these proteases also stimulate induction of Bmp4. ..
  4. Nowotschin S, Costello I, Piliszek A, Kwon G, Mao C, Klein W, et al. The T-box transcription factor Eomesodermin is essential for AVE induction in the mouse embryo. Genes Dev. 2013;27:997-1002 pubmed publisher
    ..Thus, Eomes function in the visceral endoderm (VE) initiates an instructive transcriptional program controlling AP identity. ..
  5. Kwon G, Viotti M, Hadjantonakis A. The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages. Dev Cell. 2008;15:509-20 pubmed publisher
  6. Brickman J, Jones C, Clements M, Smith J, Beddington R. Hex is a transcriptional repressor that contributes to anterior identity and suppresses Spemann organiser function. Development. 2000;127:2303-15 pubmed
    One of the earliest markers of anterior asymmetry in vertebrate embryos is the transcription factor Hex. We find that Hex is a transcriptional repressor that can be converted to an activator by fusing full length Hex to two copies of the ..
  7. Hallaq H, Pinter E, Enciso J, McGrath J, Zeiss C, Brueckner M, et al. A null mutation of Hhex results in abnormal cardiac development, defective vasculogenesis and elevated Vegfa levels. Development. 2004;131:5197-209 pubmed
    The homeobox gene Hhex has recently been shown to be essential for normal liver, thyroid and forebrain development. Hhex(-/-) mice die by mid-gestation (E14.5) and the cause of their early demise remains unclear...
  8. George A, Morse H, Justice M. The homeobox gene Hex induces T-cell-derived lymphomas when overexpressed in hematopoietic precursor cells. Oncogene. 2003;22:6764-73 pubmed
    ..in B- and T-cell leukemias and lymphomas in AKXD mice and activate two nearby genes, the divergent homeobox gene Hex and the kinesin-related spindle protein gene Eg5...
  9. Yamamoto M, Saijoh Y, Perea Gomez A, Shawlot W, Behringer R, Ang S, et al. Nodal antagonists regulate formation of the anteroposterior axis of the mouse embryo. Nature. 2004;428:387-92 pubmed

More Information


  1. Kanai Azuma M, Kanai Y, Gad J, Tajima Y, Taya C, Kurohmaru M, et al. Depletion of definitive gut endoderm in Sox17-null mutant mice. Development. 2002;129:2367-79 pubmed
    ..Our findings indicate an important role of Sox17 in endoderm development in the mouse, highlighting the idea that the molecular mechanism for endoderm formation is likely to be conserved among vertebrates. ..
  2. Perea Gomez A, Vella F, Shawlot W, Oulad Abdelghani M, Chazaud C, Meno C, et al. Nodal antagonists in the anterior visceral endoderm prevent the formation of multiple primitive streaks. Dev Cell. 2002;3:745-56 pubmed
    ..Both antagonists are also required for proper patterning of the primitive streak. ..
  3. Martinez Barbera J, Clements M, Thomas P, Rodriguez T, Meloy D, Kioussis D, et al. The homeobox gene Hex is required in definitive endodermal tissues for normal forebrain, liver and thyroid formation. Development. 2000;127:2433-45 pubmed
    The homeobox gene Hex is expressed in the anterior visceral endoderm (AVE) and rostral definitive endoderm of early mouse embryos. Later, Hex transcripts are detected in liver, thyroid and endothelial precursor cells...
  4. Bogue C, Zhang P, McGrath J, Jacobs H, Fuleihan R. Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex. Proc Natl Acad Sci U S A. 2003;100:556-61 pubmed
    b>Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells...
  5. Norris D, Brennan J, Bikoff E, Robertson E. The Foxh1-dependent autoregulatory enhancer controls the level of Nodal signals in the mouse embryo. Development. 2002;129:3455-68 pubmed
    ..The feedback loop is thus essential for maintenance of Nodal signals that selectively regulate target gene expression in a temporally and spatially controlled fashion in the mouse embryo. ..
  6. Hoodless P, Pye M, Chazaud C, Labbe E, Attisano L, Rossant J, et al. FoxH1 (Fast) functions to specify the anterior primitive streak in the mouse. Genes Dev. 2001;15:1257-71 pubmed
    ..These results show that FoxH1 functions in an activin/nodal-Smad signaling pathway that acts upstream of Foxa2 and is required specifically for patterning the APS and node in the mouse. ..
  7. Episkopou V, Arkell R, Timmons P, Walsh J, Andrew R, Swan D. Induction of the mammalian node requires Arkadia function in the extraembryonic lineages. Nature. 2001;410:825-30 pubmed
    ..Furthermore, our experiments show that Arkadia interacts genetically with the transforming growth factor (TGF)beta-like factor Nodal, implying that Nodal mediates the function of Arkadia in node induction. ..
  8. Ghosh B, Ganea G, Denson L, Iannucci R, Jacobs H, Bogue C. Immunocytochemical characterization of murine Hex, a homeobox-containing protein. Pediatr Res. 2000;48:634-8 pubmed
    ..antibody against a glutathione S:-transferase fusion protein containing the 76 COOH-terminal amino acids of Hex, a divergent homeobox gene, was raised in rabbits...
  9. Foley A, Mercola M. Heart induction by Wnt antagonists depends on the homeodomain transcription factor Hex. Genes Dev. 2005;19:387-96 pubmed
    ..revealed that Dkk-1 and other inhibitors of the canonical Wnt pathway induce the homeodomain transcription factor Hex, which is normally expressed in endoderm underlying the presumptive cardiac mesoderm in amphibian, bird, and ..
  10. Zamparini A, Watts T, Gardner C, Tomlinson S, Johnston G, Brickman J. Hex acts with beta-catenin to regulate anteroposterior patterning via a Groucho-related co-repressor and Nodal. Development. 2006;133:3709-22 pubmed
    ..There are also a number of transcription factors that feedback upon these pathways. The homeodomain protein Hex, an early marker of anterior positional information, acts as a transcriptional repressor, suppressing induction and ..
  11. Spence J, Lange A, Lin S, Kaestner K, Lowy A, Kim I, et al. Sox17 regulates organ lineage segregation of ventral foregut progenitor cells. Dev Cell. 2009;17:62-74 pubmed publisher
    ..Restricting SOX17+ biliary progenitor cells to the ventral region of the gut requires the notch effector Hes1. Our results highlight the role of Sox17 and Hes1 in patterning and morphogenetic segregation of ventral foregut lineages. ..
  12. Dunn N, Vincent S, Oxburgh L, Robertson E, Bikoff E. Combinatorial activities of Smad2 and Smad3 regulate mesoderm formation and patterning in the mouse embryo. Development. 2004;131:1717-28 pubmed
    ..Collectively, these results demonstrate that dose-dependent Smad2 and Smad3 signals cooperatively mediate cell fate decisions in the early mouse embryo. ..
  13. Kubo A, Chen V, Kennedy M, Zahradka E, Daley G, Keller G. The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation. Blood. 2005;105:4590-7 pubmed
    In this report we have investigated the role of the homeobox gene Hex in the development and differentiation of the blast colony-forming cell (BL-CFC), a progenitor with hemangioblast characteristics generated in embryonic stem (ES) cell-..
  14. Prall O, Menon M, Solloway M, Watanabe Y, Zaffran S, Bajolle F, et al. An Nkx2-5/Bmp2/Smad1 negative feedback loop controls heart progenitor specification and proliferation. Cell. 2007;128:947-59 pubmed
  15. Morrison G, Oikonomopoulou I, Migueles R, Soneji S, Livigni A, Enver T, et al. Anterior definitive endoderm from ESCs reveals a role for FGF signaling. Cell Stem Cell. 2008;3:402-15 pubmed publisher
    ..a strategy using a fluorescent reporter gene under the transcriptional control of the anterior endoderm marker Hex alongside the definitive mesendoderm marker Cxcr4...
  16. Thomas P, Brown A, Beddington R. Hex: a homeobox gene revealing peri-implantation asymmetry in the mouse embryo and an early transient marker of endothelial cell precursors. Development. 1998;125:85-94 pubmed
    The divergent homeobox gene Hex exhibits three notable expression patterns during early mouse development. Initially Hex is expressed in the primitive endoderm of the implanting blastocyst but by 5...
  17. Sekiguchi K, Kurabayashi M, Oyama Y, Aihara Y, Tanaka T, Sakamoto H, et al. Homeobox protein Hex induces SMemb/nonmuscle myosin heavy chain-B gene expression through the cAMP-responsive element. Circ Res. 2001;88:52-8 pubmed
    Recent studies have shown that the homeobox gene Hex plays an important role in inducing differentiation of vascular endothelial cells...
  18. Rodriguez T, Casey E, Harland R, Smith J, Beddington R. Distinct enhancer elements control Hex expression during gastrulation and early organogenesis. Dev Biol. 2001;234:304-16 pubmed
    ..The divergent homeobox gene Hex is expressed in the anterior visceral endoderm, in the node (transiently), and in the anterior definitive endoderm...
  19. Smith S, Tripathi R, Goodings C, Cleveland S, MATHIAS E, Hardaway J, et al. LIM domain only-2 (LMO2) induces T-cell leukemia by two distinct pathways. PLoS ONE. 2014;9:e85883 pubmed publisher
    ..penetrant T-ALL by two distinct patterns of gene expression: one in which there was concordant activation of Lyl1, Hhex, and Mycn or alternatively, with Notch1 target gene activation...
  20. Paz H, Lynch M, Bogue C, Gasson J. The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis. Blood. 2010;116:1254-62 pubmed publisher
    ..The hematopoietically expressed homeobox (Hhex) gene, a member of the homeobox gene family, is an essential regulator of embryogenesis and hematopoietic ..
  21. Morimoto R, Yamamoto A, Akimoto Y, Obinata A. Homeoprotein Hex is expressed in mouse developing chondrocytes. J Biochem. 2011;150:61-71 pubmed publisher
    ..Here, we show that a divergent-type homeobox gene, hematopoietically expressed homeobox gene (HEX), is expressed in mouse chondrogenic cell line ATDC5...
  22. Georgiades P, Rossant J. Ets2 is necessary in trophoblast for normal embryonic anteroposterior axis development. Development. 2006;133:1059-68 pubmed
    ..This study provides new insights about the genetic and cellular basis of the patterning role and development of the early trophoblast. ..
  23. Uehara M, Yashiro K, Takaoka K, Yamamoto M, Hamada H. Removal of maternal retinoic acid by embryonic CYP26 is required for correct Nodal expression during early embryonic patterning. Genes Dev. 2009;23:1689-98 pubmed publisher
    ..These observations suggest that maternal RA must be removed by embryonic CYP26 for correct Nodal expression during embryonic patterning. ..
  24. Harris Johnson K, Domyan E, Vezina C, Sun X. beta-Catenin promotes respiratory progenitor identity in mouse foregut. Proc Natl Acad Sci U S A. 2009;106:16287-92 pubmed publisher
    ..Our findings reveal an early role for beta-Catenin in the establishment of respiratory progenitors in mouse foregut endoderm. ..
  25. Vincent S, Dunn N, Hayashi S, Norris D, Robertson E. Cell fate decisions within the mouse organizer are governed by graded Nodal signals. Genes Dev. 2003;17:1646-62 pubmed
    ..These findings conclusively demonstrate that graded Nodal/Smad2 signals govern allocation of the axial mesendoderm precursors that selectively give rise to the ADE and PCP mesoderm...
  26. Martinez Barbera J, Beddington R. Getting your head around Hex and Hesx1: forebrain formation in mouse. Int J Dev Biol. 2001;45:327-36 pubmed
    ..b>Hex and Hesx1 are homeobox genes that are expressed in relevant tissues involved in anterior patterning...
  27. Alarcon V, Marikawa Y. Molecular study of mouse peri-implantation development using the in vitro culture of aggregated inner cell mass. Mol Reprod Dev. 2004;67:83-90 pubmed
    ..Taken together, we propose that the 5x ICM is a useful in vitro tool to study the mechanisms of peri-implantation development of the mouse embryo. Mol. Reprod. Dev. 67: 83-90, 2004. ..
  28. Fukusumi Y, Naruse C, Asano M. Wtap is required for differentiation of endoderm and mesoderm in the mouse embryo. Dev Dyn. 2008;237:618-29 pubmed publisher
    ..Taken together, our findings indicate that Wtap is indispensable for differentiation of mesoderm and endoderm in the mouse embryo. ..
  29. Uemura M, Hara K, Shitara H, Ishii R, Tsunekawa N, Miura Y, et al. Expression and function of mouse Sox17 gene in the specification of gallbladder/bile-duct progenitors during early foregut morphogenesis. Biochem Biophys Res Commun. 2010;391:357-63 pubmed publisher
    ..Our findings show that Sox17 functions cell-autonomously to specify gallbladder/bile-duct in the mouse embryo. ..
  30. Yamamoto M, Meno C, Sakai Y, Shiratori H, Mochida K, Ikawa Y, et al. The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse. Genes Dev. 2001;15:1242-56 pubmed
    ..These results indicate that a Nodal-FoxH1 signaling pathway plays a central role in A-P patterning and node formation in the mouse. ..
  31. Tao H, Suzuki M, Kiyonari H, Abe T, Sasaoka T, Ueno N. Mouse prickle1, the homolog of a PCP gene, is essential for epiblast apical-basal polarity. Proc Natl Acad Sci U S A. 2009;106:14426-31 pubmed publisher
    ..Our results demonstrate a role for mpk1 in AB polarity formation rather than expected role as a PCP gene. ..
  32. Burtscher I, Lickert H. Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo. Development. 2009;136:1029-38 pubmed publisher
    ..We propose that Foxa2 regulates a molecular program that induces an epithelial cellular phenotype...
  33. Kitajima S, Takagi A, Inoue T, Saga Y. MesP1 and MesP2 are essential for the development of cardiac mesoderm. Development. 2000;127:3215-26 pubmed
    ..These results strongly indicate that the defect in the cranial-cardiac mesoderm is cell-autonomous, whereas the defect in the paraxial mesoderm is a non-cell-autonomous secondary consequence. ..
  34. Marfil V, Blazquez M, Serrano F, Castell J, Bort R. Growth-promoting and tumourigenic activity of c-Myc is suppressed by Hhex. Oncogene. 2015;34:3011-22 pubmed publisher
    ..Here we show that Hhex is able to interact with the basic region/helix-loop-helix/leucine zipper of c-Myc...
  35. Polydorou C, Georgiades P. Ets2-dependent trophoblast signalling is required for gastrulation progression after primitive streak initiation. Nat Commun. 2013;4:1658 pubmed publisher
    ..We propose a model that provides a genetic explanation as to how Ets2 in trophoblast mediates the progression of gastrulation within the epiblast. ..
  36. Hansen G, Skapura D, Justice M. Genetic profile of insertion mutations in mouse leukemias and lymphomas. Genome Res. 2000;10:237-43 pubmed
  37. Engert S, Burtscher I, Liao W, Dulev S, Schotta G, Lickert H. Wnt/?-catenin signalling regulates Sox17 expression and is essential for organizer and endoderm formation in the mouse. Development. 2013;140:3128-38 pubmed publisher
  38. Nakagawa T, Abe M, Yamazaki T, Miyashita H, Niwa H, Kokubun S, et al. HEX acts as a negative regulator of angiogenesis by modulating the expression of angiogenesis-related gene in endothelial cells in vitro. Arterioscler Thromb Vasc Biol. 2003;23:231-7 pubmed
    The hematopoietically expressed homeobox (HEX) is transiently expressed in endothelial cells (ECs) during vascular formation in embryo. Here, we investigated whether HEX played any role in angiogenesis-related properties of ECs in vitro...
  39. Kalinichenko V, Zhou Y, Bhattacharyya D, Kim W, Shin B, Bambal K, et al. Haploinsufficiency of the mouse Forkhead Box f1 gene causes defects in gall bladder development. J Biol Chem. 2002;277:12369-74 pubmed
  40. Zhou X, Anderson K. Development of head organizer of the mouse embryo depends on a high level of mitochondrial metabolism. Dev Biol. 2010;344:185-95 pubmed publisher
    ..We suggest that a requirement for high levels of ATP for early forebrain patterning may contribute to certain human microcephaly syndromes...
  41. Marfil V, Moya M, Pierreux C, Castell J, Lemaigre F, Real F, et al. Interaction between Hhex and SOX13 modulates Wnt/TCF activity. J Biol Chem. 2010;285:5726-37 pubmed publisher
    ..Here we describe how the interaction between Hhex (hematopoietically expressed homeobox) and SOX13 (SRY-related high mobility group box transcription factor 13), ..
  42. Bedford F, Ashworth A, Enver T, Wiedemann L. HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human. Nucleic Acids Res. 1993;21:1245-9 pubmed
    ..The human cDNA was initially isolated from human haematopoietic tissue and denoted HEX (haematopoietically expressed homeobox)...
  43. Bort R, Martinez Barbera J, Beddington R, Zaret K. Hex homeobox gene-dependent tissue positioning is required for organogenesis of the ventral pancreas. Development. 2004;131:797-806 pubmed
    ..The homeobox gene Hex is expressed in this endoderm cell domain and in the liver and ventral pancreas buds, after organogenesis...
  44. Mavrakis K, Andrew R, Lee K, Petropoulou C, Dixon J, Navaratnam N, et al. Arkadia enhances Nodal/TGF-beta signaling by coupling phospho-Smad2/3 activity and turnover. PLoS Biol. 2007;5:e67 pubmed
    ..This mechanism can account for achieving efficient and maximum Nodal signaling during embryogenesis and for rapid resetting of target gene promoters allowing cells to respond to dynamic changes in extracellular signals. ..
  45. Kimura Yoshida C, Tian E, Nakano H, Amazaki S, Shimokawa K, Rossant J, et al. Crucial roles of Foxa2 in mouse anterior-posterior axis polarization via regulation of anterior visceral endoderm-specific genes. Proc Natl Acad Sci U S A. 2007;104:5919-24 pubmed
    ..These findings provide profound insights into conserved roles of Foxa2 transcription factors in anterior specification throughout the evolution of the chordate body plan. ..
  46. Perea Gomez A, Shawlot W, Sasaki H, Behringer R, Ang S. HNF3beta and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior-posterior polarity in the mouse embryo. Development. 1999;126:4499-511 pubmed
    ..Moreover, HNF3beta (-)(/)(-);Lim1(-)(/)(-) mutant embryos also exhibit defects in mesoderm patterning that are likely due to lack of specification of anterior primitive streak cells. ..
  47. Kolterud A, Wandzioch E, Carlsson L. Lhx2 is expressed in the septum transversum mesenchyme that becomes an integral part of the liver and the formation of these cells is independent of functional Lhx2. Gene Expr Patterns. 2004;4:521-8 pubmed
    ..expressing the mutant Lhx2 allel are present in Lhx2(-/-) livers, and expression of Hlx, hepatocyte growth factor, Hex and Prox1, genes known to be important in liver development, is independent of functional Lhx2 expression...
  48. Rakeman A, Anderson K. Axis specification and morphogenesis in the mouse embryo require Nap1, a regulator of WAVE-mediated actin branching. Development. 2006;133:3075-83 pubmed
    ..Thus, the Nap1 mutant phenotypes define the crucial roles of Nap1/WAVE-mediated actin regulation in tissue organization and establishment of the body plan of the mammalian embryo. ..
  49. Zhang J, McKenna L, Bogue C, Kaestner K. The diabetes gene Hhex maintains ?-cell differentiation and islet function. Genes Dev. 2014;28:829-34 pubmed publisher
    The homeodomain transcription factor HHEX (hematopoietically expressed homeobox) has been repeatedly linked to type 2 diabetes mellitus (T2DM) using genome-wide association studies...
  50. Yang Y, Anderson R, Klingensmith J. BMP antagonism protects Nodal signaling in the gastrula to promote the tissue interactions underlying mammalian forebrain and craniofacial patterning. Hum Mol Genet. 2010;19:3030-42 pubmed publisher
  51. Hamamoto T, Beppu H, Okada H, Kawabata M, Kitamura T, Miyazono K, et al. Compound disruption of smad2 accelerates malignant progression of intestinal tumors in apc knockout mice. Cancer Res. 2002;62:5955-61 pubmed
    ..These results indicate that loss of Smad2 does not initiate tumorigenesis by itself but accelerates malignant progression of tumors to invasive cancer in the late stages of carcinogenesis. ..
  52. Kimura C, Yoshinaga K, Tian E, Suzuki M, Aizawa S, Matsuo I. Visceral endoderm mediates forebrain development by suppressing posteriorizing signals. Dev Biol. 2000;225:304-21 pubmed
    ..These results suggest that distal visceral endoderm cells move to the future anterior side to generate a prospective forebrain territory indirectly, by preventing posteriorizing signals. ..
  53. Ghosh B, Jacobs H, Wiedemann L, Brown A, Bedford F, Nimmakayalu M, et al. Genomic structure, cDNA mapping, and chromosomal localization of the mouse homeobox gene, Hex. Mamm Genome. 1999;10:1023-5 pubmed
  54. Liu Y, Kaneda R, Leja T, Subkhankulova T, Tolmachov O, Minchiotti G, et al. Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells. Stem Cells. 2014;32:1515-26 pubmed publisher
    ..Sox17 was required not only for Hhex (a second endodermal transcription factor) but also for Cer1, a growth factor inhibitor from endoderm that, like ..
  55. Huelsken J, Vogel R, Brinkmann V, Erdmann B, Birchmeier C, Birchmeier W. Requirement for beta-catenin in anterior-posterior axis formation in mice. J Cell Biol. 2000;148:567-78 pubmed
    ..5, as visualized by the absence of Hex and Hesx1 and the mislocation of cerberus-like and Lim1 expression...
  56. Zakin L, Reversade B, Virlon B, Rusniok C, Glaser P, Elalouf J, et al. Gene expression profiles in normal and Otx2-/- early gastrulating mouse embryos. Proc Natl Acad Sci U S A. 2000;97:14388-93 pubmed
    ..Among a broader list, the study of six genes found to be differentially expressed allows defining a role for Otx2 in the orchestration of cell movements leading to the adequate organization of the embryo before gastrulation. ..
  57. Tortelote G, Hernández Hernández J, Quaresma A, Nickerson J, Imbalzano A, Rivera Perez J. Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice. Dev Biol. 2013;374:164-73 pubmed publisher
    ..5. At the molecular level, we provide evidence that Wnt3 regulates its own expression and that of other primitive streak markers via activation of the canonical Wnt signaling pathway. ..
  58. Aoyama M, Sun Wada G, Yamamoto A, Yamamoto M, Hamada H, Wada Y. Spatial restriction of bone morphogenetic protein signaling in mouse gastrula through the mVam2-dependent endocytic pathway. Dev Cell. 2012;22:1163-75 pubmed publisher
    ..We found that mVam2, which interacts with BMP type I receptor, is required for the spatiotemporal modulation of BMP signaling, via sequestration of the receptor complex in the late stages of the endocytic pathway...
  59. Sebastiano V, Dalvai M, Gentile L, Schubart K, Sutter J, Wu G, et al. Oct1 regulates trophoblast development during early mouse embryogenesis. Development. 2010;137:3551-60 pubmed publisher
    ..Our data also suggest that Oct1 is required in the embryo proper from late gastrulation stages onwards. ..
  60. Jackson J, Shields B, Shi W, Di Rago L, Metcalf D, Nicola N, et al. Hhex Regulates Hematopoietic Stem Cell Self-Renewal and Stress Hematopoiesis via Repression of Cdkn2a. Stem Cells. 2017;35:1948-1957 pubmed publisher
    The hematopoietically expressed homeobox transcription factor (Hhex) is important for the maturation of definitive hematopoietic progenitors and B-cells during development...
  61. Zakin L, De Robertis E. Inactivation of mouse Twisted gastrulation reveals its role in promoting Bmp4 activity during forebrain development. Development. 2004;131:413-24 pubmed
    ..Tsg-/-;Bmp4+/- compound mutants die at birth and display holoprosencephaly, first branchial arch and eye defects. The results show that Tsg functions to promote Bmp4 signaling during mouse head development. ..
  62. Thomas P, Brickman J, Popperl H, Krumlauf R, Beddington R. Axis duplication and anterior identity in the mouse embryo. Cold Spring Harb Symp Quant Biol. 1997;62:115-25 pubmed
  63. Hansen G, Justice M. Activation of Hex and mEg5 by retroviral insertion may contribute to mouse B-cell leukemia. Oncogene. 1999;18:6531-9 pubmed
    ..Multiple genes flank the site of viral insertion, but the expression of just two, Hex and mEg5, is significantly upregulated...
  64. Costello I, Nowotschin S, Sun X, Mould A, Hadjantonakis A, Bikoff E, et al. Lhx1 functions together with Otx2, Foxa2, and Ldb1 to govern anterior mesendoderm, node, and midline development. Genes Dev. 2015;29:2108-22 pubmed publisher
    ..These partnerships cooperatively regulate development of the anterior mesendoderm, node, and midline cell populations responsible for establishment of the left-right body axis and head formation. ..
  65. Keng V, Fujimori K, Myint Z, Tamamaki N, Nojyo Y, Noguchi T. Expression of Hex mRNA in early murine postimplantation embryo development. FEBS Lett. 1998;426:183-6 pubmed
    The onset of Hex expression and its role in early murine development was analyzed using in situ hybridization...
  66. Perea Gomez A, Camus A, Moreau A, Grieve K, Moneron G, Dubois A, et al. Initiation of gastrulation in the mouse embryo is preceded by an apparent shift in the orientation of the anterior-posterior axis. Curr Biol. 2004;14:197-207 pubmed
    ..These results reveal a level of regulation and plasticity so far unsuspected in the mouse gastrula. ..
  67. Hiramatsu R, Matsuoka T, Kimura Yoshida C, Han S, Mochida K, Adachi T, et al. External mechanical cues trigger the establishment of the anterior-posterior axis in early mouse embryos. Dev Cell. 2013;27:131-144 pubmed publisher
  68. Qiu Z, Elsayed Z, Peterkin V, Alkatib S, Bennett D, Landry J. Ino80 is essential for proximal-distal axis asymmetry in part by regulating Bmp4 expression. BMC Biol. 2016;14:18 pubmed publisher
    ..by RT-PCR shows elevated Bmp4 expression and reduced expression of distal visceral endoderm (DVE) markers Cer1, Hex, and Lefty1...
  69. Shields B, Jackson J, Metcalf D, Shi W, Huang Q, Garnham A, et al. Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a. Genes Dev. 2016;30:78-91 pubmed publisher
    ..Here we found that the hematopoietically expressed homeobox gene Hhex is overexpressed in acute myeloid leukemia (AML) and is essential for the initiation and propagation of MLL-ENL-..
  70. Guo Y, Chan R, Ramsey H, Li W, Xie X, Shelley W, et al. The homeoprotein Hex is required for hemangioblast differentiation. Blood. 2003;102:2428-35 pubmed
    ..We and others isolated a divergent homeobox gene termed Hex (or Prh) that is preferentially expressed in hematopoietic and endothelial cells...
  71. Parlato R, Rosica A, Rodriguez Mallon A, Affuso A, Postiglione M, Arra C, et al. An integrated regulatory network controlling survival and migration in thyroid organogenesis. Dev Biol. 2004;276:464-75 pubmed
    ..The experiments reported here analyze the expression of the transcription factors Titf1, Hhex, Pax8, and Foxe1 in the thyroid primordium of null mutants of each of them...
  72. Tondeleir D, Drogat B, Slowicka K, Bakkali K, Bartunkova S, Goossens S, et al. Beta-Actin Is Involved in Modulating Erythropoiesis during Development by Fine-Tuning Gata2 Expression Levels. PLoS ONE. 2013;8:e67855 pubmed publisher
    ..These results provide new molecular insights for a novel function of beta-actin in erythropoiesis by modulating the expression levels of Gata2 in vivo. ..
  73. Cong R, Jiang X, Wilson C, Hunter M, Vasavada H, Bogue C. Hhex is a direct repressor of endothelial cell-specific molecule 1 (ESM-1). Biochem Biophys Res Commun. 2006;346:535-45 pubmed
    b>Hhex encodes a homeodomain-containing protein that functions as both a transcriptional repressor and activator, and is necessary for normal embryonic development...
  74. Petryk A, Anderson R, Jarcho M, Leaf I, Carlson C, Klingensmith J, et al. The mammalian twisted gastrulation gene functions in foregut and craniofacial development. Dev Biol. 2004;267:374-86 pubmed
    ..In addition, we find defects in the foregut endoderm and a reduction in Hex expression, which may contribute to both the forebrain and BA1 defects.
  75. Flagg A, Earley J, Svensson E. FOG-2 attenuates endothelial-to-mesenchymal transformation in the endocardial cushions of the developing heart. Dev Biol. 2007;304:308-16 pubmed
    ..Taken together with GATA4's known role in promoting EMT, these results suggest that FOG-2 functions in cardiac valve formation as an attenuator of EMT by repressing GATA4 activity within the developing endocardial cushions. ..
  76. Wanderling S, Simen B, Ostrovsky O, Ahmed N, Vogen S, Gidalevitz T, et al. GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion. Mol Biol Cell. 2007;18:3764-75 pubmed
    ..The data identify insulin-like growth factor II as one developmentally important protein whose production depends on the activity of GRP94. ..
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