Gnaq

Summary

Gene Symbol: Gnaq
Description: guanine nucleotide binding protein, alpha q polypeptide
Alias: 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, GqI, guanine nucleotide-binding protein G(q) subunit alpha, G alpha q, guanine nucleotide-binding protein alpha-q
Species: mouse
Products:     Gnaq

Top Publications

  1. Offermanns S, Toombs C, Hu Y, Simon M. Defective platelet activation in G alpha(q)-deficient mice. Nature. 1997;389:183-6 pubmed
    ..G alpha(q) may thus be a new target for drugs designed to block the activation of platelets. ..
  2. Philip F, Kadamur G, Silos R, Woodson J, Ross E. Synergistic activation of phospholipase C-beta3 by Galpha(q) and Gbetagamma describes a simple two-state coincidence detector. Curr Biol. 2010;20:1327-35 pubmed publisher
    ..Any two-state enzyme is predicted to respond synergistically to multiple activating ligands if, but only if, its basal activity is strongly suppressed. ..
  3. Wettschureck N, Rutten H, Zywietz A, Gehring D, Wilkie T, Chen J, et al. Absence of pressure overload induced myocardial hypertrophy after conditional inactivation of Galphaq/Galpha11 in cardiomyocytes. Nat Med. 2001;7:1236-40 pubmed
    ..transgenic overexpression of alpha1-adrenergic or angiotensin (AT1)-receptors as well as of the Gq alpha-subunit, Galphaq, results in myocardial hypertrophy...
  4. Lyon A, Tesmer V, Dhamsania V, Thal D, Gutierrez J, Chowdhury S, et al. An autoinhibitory helix in the C-terminal region of phospholipase C-? mediates G?q activation. Nat Struct Mol Biol. 2011;18:999-1005 pubmed publisher
    ..G?q binding requires displacement of the autoinhibitory helix from the catalytic core, thus providing an allosteric mechanism for activation of PLC?. ..
  5. Ogata N, Kawaguchi H, Chung U, Roth S, Segre G. Continuous activation of G alpha q in osteoblasts results in osteopenia through impaired osteoblast differentiation. J Biol Chem. 2007;282:35757-64 pubmed
    ..We propose that continuous activation of the G alpha(q) signal in osteoblasts plays a crucial, previously unrecognized role in bone formation. ..
  6. Vogt S, Grosse R, Schultz G, Offermanns S. Receptor-dependent RhoA activation in G12/G13-deficient cells: genetic evidence for an involvement of Gq/G11. J Biol Chem. 2003;278:28743-9 pubmed
    ..In pertussis toxin-treated Galpha12/Galpha13-deficient as well as in Galphaq/Galpha11-deficient mouse embryonic fibroblasts (MEFs), in which coupling of receptors is restricted to Gq/G11 and ..
  7. Offermanns S, Zhao L, Gohla A, Sarosi I, Simon M, Wilkie T. Embryonic cardiomyocyte hypoplasia and craniofacial defects in G alpha q/G alpha 11-mutant mice. EMBO J. 1998;17:4304-12 pubmed
    ..Knockout mice carrying inactivating mutations in both of the widely expressed G alpha q class genes, G alpha q and G alpha 11, demonstrate that at least two active alleles of these genes are required ..
  8. Shankaranarayanan A, Thal D, Tesmer V, Roman D, Neubig R, Kozasa T, et al. Assembly of high order G alpha q-effector complexes with RGS proteins. J Biol Chem. 2008;283:34923-34 pubmed publisher
  9. Waldo G, Ricks T, Hicks S, Cheever M, Kawano T, Tsuboi K, et al. Kinetic scaffolding mediated by a phospholipase C-beta and Gq signaling complex. Science. 2010;330:974-80 pubmed publisher
    ..Mutations within this domain dramatically delay signal termination in vitro and in vivo. Consequently, this work suggests a dynamic catch-and-release mechanism used to sharpen spatiotemporal signals mediated by diverse sensory inputs. ..

More Information

Publications89

  1. Offermanns S, Hashimoto K, Watanabe M, Sun W, Kurihara H, Thompson R, et al. Impaired motor coordination and persistent multiple climbing fiber innervation of cerebellar Purkinje cells in mice lacking Galphaq. Proc Natl Acad Sci U S A. 1997;94:14089-94 pubmed
    Mice lacking the alpha-subunit of the heterotrimeric guanine nucleotide binding protein Gq (Galphaq) are viable but suffer from ataxia with typical signs of motor discoordination...
  2. Kamitani S, Ao S, Toshima H, Tachibana T, Hashimoto M, Kitadokoro K, et al. Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated ? subunit of the heterotrimeric GTPase Gq. FEBS J. 2011;278:2702-12 pubmed publisher
    ..These results not only support previous observations on toxicity, but also provide insights into the enzymatic nature of PMT. In addition, we present several lines of evidence that G?(11), as well as G?(q), could be a substrate for PMT. ..
  3. Ogata N, Shinoda Y, Wettschureck N, Offermanns S, Takeda S, Nakamura K, et al. G alpha(q) signal in osteoblasts is inhibitory to the osteoanabolic action of parathyroid hormone. J Biol Chem. 2011;286:13733-40 pubmed publisher
    ..Hence, the G?(q) signal plays an inhibitory role in the PTH osteoanabolic action, suggesting that its suppression may lead to a novel treatment in combination with PTH against osteoporosis. ..
  4. Howes A, Arthur J, Zhang T, Miyamoto S, Adams J, Dorn G, et al. Akt-mediated cardiomyocyte survival pathways are compromised by G alpha q-induced phosphoinositide 4,5-bisphosphate depletion. J Biol Chem. 2003;278:40343-51 pubmed
    ..the wild type alpha subunit of Gq (GqWT) in cardiomyocytes induces hypertrophy, whereas a constitutively active G alpha q subunit (GqQ209L) induces apoptosis...
  5. Offermanns S, Mancino V, Revel J, Simon M. Vascular system defects and impaired cell chemokinesis as a result of Galpha13 deficiency. Science. 1997;275:533-6 pubmed
    ..These results demonstrate that Galpha13 participates in the regulation of cell movement in response to specific ligands, as well as in developmental angiogenesis. ..
  6. Borchers M, Justice P, Ansay T, Mancino V, McGarry M, Crosby J, et al. Gq signaling is required for allergen-induced pulmonary eosinophilia. J Immunol. 2002;168:3543-9 pubmed
  7. Strathmann M, Simon M. G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates. Proc Natl Acad Sci U S A. 1990;87:9113-7 pubmed
    ..We have now obtained cDNA clones encoding two murine alpha subunits, G alpha q and G alpha 11, that are 88% identical...
  8. Winter S, Brunk I, Walther D, Höltje M, Jiang M, Peter J, et al. Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence. J Neurosci. 2005;25:4672-80 pubmed
    ..Thus, Galpha(o2) acts on a putative regulatory chloride binding domain that appears to modulate transport activity of vesicular glutamate transporters. ..
  9. Höltje M, Winter S, Walther D, Pahner I, Hörtnagl H, Ottersen O, et al. The vesicular monoamine content regulates VMAT2 activity through Galphaq in mouse platelets. Evidence for autoregulation of vesicular transmitter uptake. J Biol Chem. 2003;278:15850-8 pubmed
    ..It is concluded that in platelets the vesicle itself regulates transmitter transporter activity via its content and vesicle-associated Galpha(q). ..
  10. Rashid A, So C, Kong M, Furtak T, El Ghundi M, Cheng R, et al. D1-D2 dopamine receptor heterooligomers with unique pharmacology are coupled to rapid activation of Gq/11 in the striatum. Proc Natl Acad Sci U S A. 2007;104:654-9 pubmed
  11. García Hoz C, Sánchez Fernández G, García Escudero R, Fernandez Velasco M, Palacios García J, Ruiz Meana M, et al. Protein kinase C (PKC)ζ-mediated Gαq stimulation of ERK5 protein pathway in cardiomyocytes and cardiac fibroblasts. J Biol Chem. 2012;287:7792-802 pubmed publisher
    ..Taken together, our results show that PKCζ is essential for Gq-dependent ERK5 activation in cardiomyocytes and cardiac fibroblasts and indicate a key cardiac physiological role for the Gα(q)/PKCζ/ERK5 signaling axis. ..
  12. Shi G, Partida Sanchez S, Misra R, Tighe M, Borchers M, Lee J, et al. Identification of an alternative G{alpha}q-dependent chemokine receptor signal transduction pathway in dendritic cells and granulocytes. J Exp Med. 2007;204:2705-18 pubmed
  13. Wirth A, Benyo Z, Lukasova M, Leutgeb B, Wettschureck N, Gorbey S, et al. G12-G13-LARG-mediated signaling in vascular smooth muscle is required for salt-induced hypertension. Nat Med. 2008;14:64-8 pubmed
    ..This identifies the G12-G13-LARG-mediated signaling pathway as a new target for antihypertensive therapies that would be expected to leave normal blood pressure regulation unaffected. ..
  14. Ngai J, Methi T, Andressen K, Levy F, Torgersen K, Vang T, et al. The heterotrimeric G-protein alpha-subunit Galphaq regulates TCR-mediated immune responses through an Lck-dependent pathway. Eur J Immunol. 2008;38:3208-18 pubmed publisher
    ..TCR/CD3 crosslinking resulted in activation of both Galphaq and Galphas, but not Galphai-2...
  15. García Hoz C, Sánchez Fernández G, Diaz Meco M, Moscat J, Mayor F, Ribas C. G alpha(q) acts as an adaptor protein in protein kinase C zeta (PKCzeta)-mediated ERK5 activation by G protein-coupled receptors (GPCR). J Biol Chem. 2010;285:13480-9 pubmed publisher
    ..These data put forward a novel function of G alpha(q) as a scaffold protein involved in the modulation of the ERK5 cascade by GPCR that could be relevant in G(q)-mediated physiological functions. ..
  16. Gu J, Muller S, Mancino V, Offermanns S, Simon M. Interaction of G alpha(12) with G alpha(13) and G alpha(q) signaling pathways. Proc Natl Acad Sci U S A. 2002;99:9352-7 pubmed
    ..These data indicate that the G alpha(12)-mediated signaling pathway functionally interacts not only with the G alpha(13)- but also with the G alpha(q/11)-mediated signaling systems. ..
  17. Miura M, Watanabe M, Offermanns S, Simon M, Kano M. Group I metabotropic glutamate receptor signaling via Galpha q/Galpha 11 secures the induction of long-term potentiation in the hippocampal area CA1. J Neurosci. 2002;22:8379-90 pubmed
    ..cascade in hippocampal long-term potentiation (LTP) by using null-mutant mice lacking the alpha subunit of Gq (Galphaq) or G11 (Galpha11)...
  18. Kleppisch T, Voigt V, Allmann R, Offermanns S. G(alpha)q-deficient mice lack metabotropic glutamate receptor-dependent long-term depression but show normal long-term potentiation in the hippocampal CA1 region. J Neurosci. 2001;21:4943-8 pubmed
    ..These results show that G(q) plays a crucial role in the mGluR-dependent LTD, whereas hippocampal LTP is not affected by the lack of a single member of the G(q) family. ..
  19. Moers A, Wettschureck N, Grüner S, Nieswandt B, Offermanns S. Unresponsiveness of platelets lacking both Galpha(q) and Galpha(13). Implications for collagen-induced platelet activation. J Biol Chem. 2004;279:45354-9 pubmed
  20. Jordan T, Li J, Jiang H, DiMario J. Repression of slow myosin heavy chain 2 gene expression in fast skeletal muscle fibers by muscarinic acetylcholine receptor and G(alpha)q signaling. J Cell Biol. 2003;162:843-50 pubmed
    ..These data suggest that slow MyHC2 repression in innervated fast PM fibers is mediated by cell signaling involving mAchRs, G(alpha)q, and PKC. ..
  21. Shin D, Lin S, Fu Y, Ptacek L. Very large G protein-coupled receptor 1 regulates myelin-associated glycoprotein via G?s/G?q-mediated protein kinases A/C. Proc Natl Acad Sci U S A. 2013;110:19101-6 pubmed publisher
    ..Further work is required to determine whether MAG dysregulation is a cause or consequence of audiogenic epilepsy and whether there are other pathways regulated by MASS1. ..
  22. Sassmann A, Gier B, Grone H, Drews G, Offermanns S, Wettschureck N. The Gq/G11-mediated signaling pathway is critical for autocrine potentiation of insulin secretion in mice. J Clin Invest. 2010;120:2184-93 pubmed publisher
    ..We show here that beta cell-specific inactivation of the genes encoding the G protein alpha-subunits Galphaq and Galpha11 resulted in impaired glucose tolerance and insulin secretion in mice...
  23. Lai W, Cai Y, Zhou J, Chen S, Qin C, Yang C, et al. Deficiency of the G protein Gαq ameliorates experimental autoimmune encephalomyelitis with impaired DC-derived IL-6 production and Th17 differentiation. Cell Mol Immunol. 2017;14:557-567 pubmed publisher
    ..In summary, our data highlighted the critical role of Gαq in regulating Th17 differentiation and MS pathogenesis. ..
  24. Wang D, Liu Y, Li Y, He Y, Zhang J, Shi G. G?q Regulates the Development of Rheumatoid Arthritis by Modulating Th1 Differentiation. Mediators Inflamm. 2017;2017:4639081 pubmed publisher
    ..By using G?q knockout (Gnaq-/-) mice, we demonstrated that loss of G?q led to enhanced Th1 cell differentiation...
  25. Zhang Y, Kim J, Horst O, Nakano Y, Zhu L, Radlanski R, et al. Fluorosed mouse ameloblasts have increased SATB1 retention and G?q activity. PLoS ONE. 2014;9:e103994 pubmed publisher
  26. White C, Coetsee M, Morgan K, Flanagan C, Millar R, Lu Z. A crucial role for Galphaq/11, but not Galphai/o or Galphas, in gonadotropin-releasing hormone receptor-mediated cell growth inhibition. Mol Endocrinol. 2008;22:2520-30 pubmed publisher
  27. Rambhatla L, Patel B, Dhanasekaran N, Latham K. Analysis of G protein alpha subunit mRNA abundance in preimplantation mouse embryos using a rapid, quantitative RT-PCR approach. Mol Reprod Dev. 1995;41:314-24 pubmed
    ..approach, we analyzed the patterns of expression of nine G protein alpha subunit mRNAs (G alpha s, G alpha i, G alpha q, G alpha o, and G alpha 11-15) in oocytes, eggs, and preimplantation embryos from fertilization to the ..
  28. Charpentier T, Waldo G, Lowery Gionta E, Krajewski K, Strahl B, Kash T, et al. Potent and Selective Peptide-based Inhibition of the G Protein G?q. J Biol Chem. 2016;291:25608-25616 pubmed
  29. Tepe N, Liggett S. Transgenic replacement of type V adenylyl cyclase identifies a critical mechanism of beta-adrenergic receptor dysfunction in the G alpha q overexpressing mouse. FEBS Lett. 1999;458:236-40 pubmed
    Chronic activation of Gq coupled receptors, or overexpression of G alpha q, in cardiomyocytes results in hypertrophy, enhanced expression of fetal genes, decreased basal and beta-adrenergic receptor (beta AR) stimulated adenylyl cyclase (..
  30. Liapis E, Sandiford S, Wang Q, Gaidosh G, Motti D, Levay K, et al. Subcellular localization of regulator of G protein signaling RGS7 complex in neurons and transfected cells. J Neurochem. 2012;122:568-81 pubmed publisher
    ..RGS7 also localized in centrosomes. Structure-function analysis showed that the punctate localization was mediated by the DEP/DHEX domains, and centrosomal localization was dependent on the DHEX domain. ..
  31. Dettlaff Swiercz D, Wettschureck N, Moers A, Huber K, Offermanns S. Characteristic defects in neural crest cell-specific Galphaq/Galpha11- and Galpha12/Galpha13-deficient mice. Dev Biol. 2005;282:174-82 pubmed
    ..In contrast, ET-3/ET(B)-mediated development of neural crest-derived melanocytes and enteric neurons appears to involve G-proteins different from G(q)/G(11)/G(12)/G(13). ..
  32. Lee M, Tasaki T, Moroi K, An J, Kimura S, Davydov I, et al. RGS4 and RGS5 are in vivo substrates of the N-end rule pathway. Proc Natl Acad Sci U S A. 2005;102:15030-5 pubmed
  33. Goulimari P, Kitzing T, Knieling H, Brandt D, Offermanns S, Grosse R. Galpha12/13 is essential for directed cell migration and localized Rho-Dia1 function. J Biol Chem. 2005;280:42242-51 pubmed
    ..Here we show that Galpha12/13, but not Galphaq/11 or Galphai, is indispensable for coordinated and directed cell migration...
  34. MacDonald D, Weerapura M, Beazely M, Martin L, Czerwinski W, Roder J, et al. Modulation of NMDA receptors by pituitary adenylate cyclase activating peptide in CA1 neurons requires G alpha q, protein kinase C, and activation of Src. J Neurosci. 2005;25:11374-84 pubmed
    ..2003). However, the major hippocampal PACAP1 receptor (PAC1R) also signals via Galphaq subunits and protein kinase C (PKC)...
  35. Edes I, Toth A, Csanyi G, Lomnicka M, Chłopicki S, Edes I, et al. Late-stage alterations in myofibrillar contractile function in a transgenic mouse model of dilated cardiomyopathy (Tgalphaq*44). J Mol Cell Cardiol. 2008;45:363-72 pubmed publisher
    ..with the progression of dilated cardiomyopathy (DCM) in a transgenic mouse line overexpressing the activated Galphaq protein (Tgalphaq*44)...
  36. Graham D, Buendia M, Chapman M, Durai H, Stanwood G. Deletion of Gαq in the telencephalon alters specific neurobehavioral outcomes. Synapse. 2015;69:434-45 pubmed publisher
    ..Taken together, our results define specific modulation of spatial working memory and psychostimulant responses through disruptions in G(αq) signaling within cerebral cortical glutamatergic neurons. ..
  37. Klages B, Brandt U, Simon M, Schultz G, Offermanns S. Activation of G12/G13 results in shape change and Rho/Rho-kinase-mediated myosin light chain phosphorylation in mouse platelets. J Cell Biol. 1999;144:745-54 pubmed
    ..In contrast to thrombin, the TXA2 mimetic U46619 led to the selective activation of G12 and G13 in Galphaq-deficient platelets indicating that these G proteins mediate TXA2 receptor-induced shape change...
  38. Semack A, Sandhu M, Malik R, Vaidehi N, Sivaramakrishnan S. Structural Elements in the G?s and G?q C Termini That Mediate Selective G Protein-coupled Receptor (GPCR) Signaling. J Biol Chem. 2016;291:17929-40 pubmed publisher
    ..Overall, our study highlights the synergy in molecular dynamics and FRET-based approaches to dissect the structural basis of selective G protein interactions. ..
  39. Schuller U, Lamp E, Schilling K. Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex. Histochem Cell Biol. 2001;116:149-59 pubmed
    ..The mRNAs encoding subunits Galphaq, Galphao, Galphai-2, and Galphaz are expressed in the cerebellar anlage at least from embryonic day 14 onward, ..
  40. Nakata A, Urano D, Fujii Kuriyama Y, Mizuno N, Tago K, Itoh H. G-protein signalling negatively regulates the stability of aryl hydrocarbon receptor. EMBO Rep. 2009;10:622-8 pubmed publisher
    ..However, in the presence of active Galpha13, AhR fails to form the active transcriptional complex. Taken together, we propose a new negative regulation of dioxin signalling by the G protein. ..
  41. Korhonen H, Fisslthaler B, Moers A, Wirth A, Habermehl D, Wieland T, et al. Anaphylactic shock depends on endothelial Gq/G11. J Exp Med. 2009;206:411-20 pubmed publisher
    ..This identifies endothelial G(q)/G(11)-mediated signaling as a critical mediator of fatal systemic anaphylaxis and, hence, as a potential new target to prevent or treat anaphylactic reactions. ..
  42. Reuter H, Seuthe K, Korkmaz Y, Grönke S, Hoyer D, Rottlaender D, et al. The G protein G?11 is essential for hypertrophic signalling in diabetic myocardium. Int J Cardiol. 2013;167:1476-85 pubmed publisher
    ..Stimulation of hypertrophic signalling through PKC-?, PKC-?, PKC-?, and NF-?B appears to be receptor-dependent, whereas PKC-? is activated by hyperglycemia, independent of G?11. ..
  43. Kim S, Kunapuli S. Negative regulation of Gq-mediated pathways in platelets by G(12/13) pathways through Fyn kinase. J Biol Chem. 2011;286:24170-9 pubmed publisher
    ..We conclude that SFKs, especially Fyn, activated downstream of G(12/13) negatively regulate platelet responses by inhibiting intracellular calcium mobilization and PKC activation through G(q) pathways. ..
  44. Geng H, Lan R, Singha P, Gilchrist A, Weinreb P, Violette S, et al. Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and G?q-mediated Rho and ?v?6 integrin-dependent activation of TGF-?. Am J Pathol. 2012;181:1236-49 pubmed publisher
    ..We suggest that LPA-initiated autocrine signaling is a potentially important mechanism that gives rise to paracrine profibrotic signaling in injured kidney tubule cells. ..
  45. Pi M, Chen L, Huang M, Luo Q, Quarles L. Parathyroid-specific interaction of the calcium-sensing receptor and G alpha q. Kidney Int. 2008;74:1548-56 pubmed publisher
    ..parathyroid gland functions, including hormone secretion, gene transcription, and chief cell hyperplasia through G alpha q- and G alpha i-dependent signaling pathways...
  46. Niizeki T, Takeishi Y, Kitahara T, Arimoto T, Ishino M, Bilim O, et al. Diacylglycerol kinase-epsilon restores cardiac dysfunction under chronic pressure overload: a new specific regulator of Galpha(q) signaling cascade. Am J Physiol Heart Circ Physiol. 2008;295:H245-55 pubmed publisher
    ..DGKepsilon may be a novel therapeutic target to prevent cardiac hypertrophy and progression to heart failure. ..
  47. Rieken S, Herroeder S, Sassmann A, Wallenwein B, Moers A, Offermanns S, et al. Lysophospholipids control integrin-dependent adhesion in splenic B cells through G(i) and G(12)/G(13) family G-proteins but not through G(q)/G(11). J Biol Chem. 2006;281:36985-92 pubmed
    ..Taken together, this study shows that lysophospholipids regulate integrin-mediated adhesion of splenic B cells to ICAM-1 through G(i) and G(12)/G(13) family G-proteins but not through G(q)/G(11). ..
  48. Xiang B, Zhang G, Stefanini L, Bergmeier W, Gartner T, Whiteheart S, et al. The Src family kinases and protein kinase C synergize to mediate Gq-dependent platelet activation. J Biol Chem. 2012;287:41277-87 pubmed publisher
    ..These data indicate that Ca(2+)/SFKs/PI3K and PKC represent two alternative signaling pathways mediating G(q)-dependent platelet activation. ..
  49. Hu P, Liu J, Yasrebi A, Gotthardt J, Bello N, Pang Z, et al. Gq Protein-Coupled Membrane-Initiated Estrogen Signaling Rapidly Excites Corticotropin-Releasing Hormone Neurons in the Hypothalamic Paraventricular Nucleus in Female Mice. Endocrinology. 2016;157:3604-20 pubmed publisher
    ..Our findings provide a potential underlying mechanism for E2's involvement in the pathophysiology of HPA-associated mood disorders. ..
  50. Boularan C, Hwang I, Kamenyeva O, Park C, Harrison K, Huang Z, et al. B Lymphocyte-Specific Loss of Ric-8A Results in a Gα Protein Deficit and Severe Humoral Immunodeficiency. J Immunol. 2015;195:2090-102 pubmed publisher
    ..In B lymphocytes, Ric-8A is essential for normal Gα protein levels and is required for B cell differentiation, trafficking, and Ab responses. ..
  51. Zhang X, Mak S, Li L, Parra A, Denlinger B, Belmonte C, et al. Direct inhibition of the cold-activated TRPM8 ion channel by G?q. Nat Cell Biol. 2012;14:851-8 pubmed publisher
    ..This signalling mechanism may underlie the abnormal cold sensation caused by inflammation. ..
  52. Walensky L, Snyder S. Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm. J Cell Biol. 1995;130:857-69 pubmed
    ..Peptide antibodies colocalized G alpha q/11 and the beta 1 isoform of phospholipase C (PLC beta 1) to the anterior acrosomal region of mouse sperm...
  53. Hoyer D, Grönke S, Frank K, Addicks K, Wettschureck N, Offermanns S, et al. Diabetes-related defects in sarcoplasmic Ca2+ release are prevented by inactivation of G(alpha)11 and G(alpha)q in murine cardiomyocytes. Mol Cell Biochem. 2010;341:235-44 pubmed publisher
    ..6, annexin A7, and sorcin. ..
  54. Stanislaus D, Janovick J, Ji T, Wilkie T, Offermanns S, Conn P. Gonadotropin and gonadal steroid release in response to a gonadotropin-releasing hormone agonist in Gqalpha and G11alpha knockout mice. Endocrinology. 1998;139:2710-7 pubmed
  55. Kuijpers M, Schulte V, Bergmeier W, Lindhout T, Brakebusch C, Offermanns S, et al. Complementary roles of glycoprotein VI and alpha2beta1 integrin in collagen-induced thrombus formation in flowing whole blood ex vivo. FASEB J. 2003;17:685-7 pubmed
  56. Friedman E, Jin L, Cai G, Hollon T, Drago J, Sibley D, et al. D1-like dopaminergic activation of phosphoinositide hydrolysis is independent of D1A dopamine receptors: evidence from D1A knockout mice. Mol Pharmacol. 1997;51:6-11 pubmed
    ..or frontal cortex membranes to G alpha s is markedly reduced, although coupling of [3H]SCH23390 binding sites to G alpha q was unaltered in tissue taken from D1A mutant mice compared with control animals...
  57. Nonne C, Lenain N, Hechler B, Mangin P, Cazenave J, Gachet C, et al. Importance of platelet phospholipase Cgamma2 signaling in arterial thrombosis as a function of lesion severity. Arterioscler Thromb Vasc Biol. 2005;25:1293-8 pubmed
    ..However, PLCbeta transduces signals from Galphaq protein-coupled receptors for soluble agonists (P2y1, TxA2/TP, and thrombin/PAR)...
  58. Woulfe D, Jiang H, Morgans A, Monks R, Birnbaum M, Brass L. Defects in secretion, aggregation, and thrombus formation in platelets from mice lacking Akt2. J Clin Invest. 2004;113:441-50 pubmed
    ..The contribution of Akt1 is less evident except in the setting in which Akt2 is absent. ..
  59. Ansari M, Dhar M, Muthukrishnan V, Morton T, Bakht N, Jacobson J. Administration of antisense oligonucleotides to Galpha(Q/11) reduces the severity of murine lupus. Biochimie. 2003;85:627-32 pubmed
    ..A trend toward prolonged survival was also noted. In vitro co-culture experiments demonstrated that antisense to Galpha(q/11) significantly inhibited IL-6 production compared to control. ..
  60. Esposito G, Prasad S, Rapacciuolo A, Mao L, Koch W, Rockman H. Cardiac overexpression of a G(q) inhibitor blocks induction of extracellular signal-regulated kinase and c-Jun NH(2)-terminal kinase activity in in vivo pressure overload. Circulation. 2001;103:1453-8 pubmed
    ..mice: one overexpressing a carboxyl-terminal peptide of Galpha(q) that inhibits G(q)-mediated signaling (TG GqI mouse) and another overexpressing a carboxyl-terminal peptide of beta-adrenergic receptor kinase-1 that inhibits ..
  61. Breen S, Andric N, Ping T, Xie F, Offermans S, Gossen J, et al. Ovulation involves the luteinizing hormone-dependent activation of G(q/11) in granulosa cells. Mol Endocrinol. 2013;27:1483-91 pubmed publisher
    ..This is the first conclusive evidence for the physiological importance of the activation of Gq/11 by the LHR and for the involvement of G?q/11 in ovulation. ..
  62. Borchers M, Biechele T, Justice J, Ansay T, Cormier S, Mancino V, et al. Methacholine-induced airway hyperresponsiveness is dependent on Galphaq signaling. Am J Physiol Lung Cell Mol Physiol. 2003;285:L114-20 pubmed
    ..b>Galphaq-containing G proteins are proposed to be a prominent signaling pathway, and the availability of knockout mice ..
  63. Nieswandt B, Schulte V, Zywietz A, Gratacap M, Offermanns S. Costimulation of Gi- and G12/G13-mediated signaling pathways induces integrin alpha IIbbeta 3 activation in platelets. J Biol Chem. 2002;277:39493-8 pubmed
  64. Palumbo J, Talmage K, Massari J, La Jeunesse C, Flick M, Kombrinck K, et al. Tumor cell-associated tissue factor and circulating hemostatic factors cooperate to increase metastatic potential through natural killer cell-dependent and-independent mechanisms. Blood. 2007;110:133-41 pubmed
    ..However, TF also supported the early success of micrometastases through an additional mechanism independent of natural killer cells, but coupled to circulating prothrombin. ..
  65. Doçi C, Mikelis C, Callejas Valera J, Hansen K, Molinolo A, Inoue A, et al. Epidermal loss of G?q confers a migratory and differentiation defect in keratinocytes. PLoS ONE. 2017;12:e0173692 pubmed publisher
    ..Emergence of a malignant phenotype may therefore arise from both under- and overexpression of G?11/q signaling, implicating its upstream regulation as a potential therapeutic target in a host of pathologic conditions. ..
  66. Lancel S, Qin F, Lennon S, Zhang J, Tong X, Mazzini M, et al. Oxidative posttranslational modifications mediate decreased SERCA activity and myocyte dysfunction in Galphaq-overexpressing mice. Circ Res. 2010;107:228-32 pubmed publisher
    ..Mice with cardiac myocyte-specific overexpression of Galphaq develop progressive left ventricular failure associated with myocyte contractile dysfunction and calcium ..
  67. Zhu S, McGrath B, Bai Y, Tang X, Cavener D. PERK regulates Gq protein-coupled intracellular Ca2+ dynamics in primary cortical neurons. Mol Brain. 2016;9:87 pubmed
  68. Schumacher S, Gao E, Cohen M, Lieu M, Chuprun J, Koch W. A peptide of the RGS domain of GRK2 binds and inhibits G?(q) to suppress pathological cardiac hypertrophy and dysfunction. Sci Signal. 2016;9:ra30 pubmed publisher
    ..The ?ARKrgs peptide, but not endogenous GRK2, interacted with G?(q) and interfered with signaling through this G protein. These data support the development of GRK2-based therapeutic approaches to prevent hypertrophy and heart failure. ..
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    ..in the regulation of neuronal excitability in mice that selectively lack the alpha-subunits of Gq and G11, G alpha q and G alpha 11, respectively, in forebrain principal neurons...
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    ..Together, these results reveal a Wnt-dependent osteogenic mechanism, and they provide a potential target pathway for designing therapeutics to promote bone formation. ..
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    ..induced renal artery stenosis for 8 weeks in control mice and mice expressing a peptide inhibitor of Gq signaling (GqI) using a 2 kidney, 1 clip renal artery stenosis model...
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    ..This study supports a key role for the RhoA signalling circuitry in vascular permeability, thereby identifying novel pharmacological targets for many human diseases characterized by aberrant vascular leakage. ..
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    ..These results provide to our knowledge one of the first examples of the functional specificity of Galpha(q) and Galpha(11) in central neurons. ..
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    ..Thus, structural rearrangement analyses by FRET revealed that Gq coupling stabilizes the active conformation of the M(1)R and also suggested that depolarization accelerates the transition from quiescent to activation conformation. ..
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    ..These observations may have important implications for a potential use of anti-GP VI-based therapeutics in the prevention of cardiovascular disease. ..
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    ..Together, our data demonstrate that PIEZO1 is required for the regulation of NO formation, vascular tone, and blood pressure. ..
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