Gene Symbol: Gata1
Description: GATA binding protein 1
Alias: Gata-1, Gf-1, eryf1, erythroid transcription factor, GATA-binding factor 1, NF-E1 DNA-binding protein
Species: mouse
Products:     Gata1

Top Publications

  1. Suzuki N, Suwabe N, Ohneda O, Obara N, Imagawa S, Pan X, et al. Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels. Blood. 2003;102:3575-83 pubmed
    ..This study also presents an efficient method for enriching the CFU-E and BFU-E from mouse hematopoietic tissues. ..
  2. Cantor A, Iwasaki H, Arinobu Y, Moran T, Shigematsu H, Sullivan M, et al. Antagonism of FOG-1 and GATA factors in fate choice for the mast cell lineage. J Exp Med. 2008;205:611-24 pubmed publisher
    ..They also provide evidence for lineage instability during early stages of hematopoietic lineage commitment. ..
  3. Meier N, Krpic S, Rodriguez P, Strouboulis J, Monti M, Krijgsveld J, et al. Novel binding partners of Ldb1 are required for haematopoietic development. Development. 2006;133:4913-23 pubmed
    ..It interacts with Gata1, Tal1, E2A and Lmo2 to form a transcription factor complex regulating late erythroid genes...
  4. Huang Z, Dore L, Li Z, Orkin S, Feng G, Lin S, et al. GATA-2 reinforces megakaryocyte development in the absence of GATA-1. Mol Cell Biol. 2009;29:5168-80 pubmed publisher
    ..leukemia in children with Down syndrome, a malignancy that is defined by the combination of trisomy 21 and a GATA1 mutation...
  5. Martin D, Orkin S. Transcriptional activation and DNA binding by the erythroid factor GF-1/NF-E1/Eryf 1. Genes Dev. 1990;4:1886-98 pubmed
    ..The complex activation and DNA-binding properties of GF-1 are likely to contribute to the ability of this single protein to participate widely in gene expression throughout erythroid development. ..
  6. Vyas P, Ault K, Jackson C, Orkin S, Shivdasani R. Consequences of GATA-1 deficiency in megakaryocytes and platelets. Blood. 1999;93:2867-75 pubmed
    ..Our findings indicate that GATA-1 serves multiple functions in megakaryocyte development, influencing both cellular growth and maturation. ..
  7. Collavin L, Gostissa M, Avolio F, Secco P, Ronchi A, Santoro C, et al. Modification of the erythroid transcription factor GATA-1 by SUMO-1. Proc Natl Acad Sci U S A. 2004;101:8870-5 pubmed
    ..Here, we demonstrate that the erythroid transcription factor GATA-1 is sumoylated in vitro and in vivo and map the single lysine residue involved in SUMO-1 ..
  8. Li H, MacLean G, Cameron D, Clagett Dame M, Petkovich M. Cyp26b1 expression in murine Sertoli cells is required to maintain male germ cells in an undifferentiated state during embryogenesis. PLoS ONE. 2009;4:e7501 pubmed publisher
  9. Cheng Y, Wu W, Kumar S, Yu D, Deng W, Tripic T, et al. Erythroid GATA1 function revealed by genome-wide analysis of transcription factor occupancy, histone modifications, and mRNA expression. Genome Res. 2009;19:2172-84 pubmed publisher
    The transcription factor GATA1 regulates an extensive program of gene activation and repression during erythroid development...

More Information


  1. Liu L, Du Z, Zhao H, Liu X, Huang X, Shen J, et al. T to C substitution at -175 or -173 of the gamma-globin promoter affects GATA-1 and Oct-1 binding in vitro differently but can independently reproduce the hereditary persistence of fetal hemoglobin phenotype in transgenic mice. J Biol Chem. 2005;280:7452-9 pubmed
    ..Both the -173 and -175 T to C substitutions may disrupt the complex assembly and result in the reactivation of the gamma-globin gene in adult erythrocytes. ..
  2. Fujiwara Y, Browne C, Cunniff K, Goff S, Orkin S. Arrested development of embryonic red cell precursors in mouse embryos lacking transcription factor GATA-1. Proc Natl Acad Sci U S A. 1996;93:12355-8 pubmed
    ..Moreover, the recovery of GATA-1+/- mice from anemia seen at birth provides evidence indicating a role for GATA-1 at the hematopoietic progenitor cell level. ..
  3. Vannucchi A, Bianchi L, Cellai C, Paoletti F, Carrai V, Calzolari A, et al. Accentuated response to phenylhydrazine and erythropoietin in mice genetically impaired for their GATA-1 expression (GATA-1(low) mice). Blood. 2001;97:3040-50 pubmed
    ..In conclusion, the GATA-1(low) mutation increases the magnitude of the response to erythroid stimuli as a consequence of the expansion of the erythroid progenitor cells in their spleen. ..
  4. Anguita E, Hughes J, Heyworth C, Blobel G, Wood W, Higgs D. Globin gene activation during haemopoiesis is driven by protein complexes nucleated by GATA-1 and GATA-2. EMBO J. 2004;23:2841-52 pubmed
  5. Chou S, Khandros E, Bailey L, Nichols K, Vakoc C, Yao Y, et al. Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate. Blood. 2009;114:983-94 pubmed publisher
    ..We used immortalized Gata1(-) erythromegakaryocytic progenitor cells to study how PU...
  6. Weiss M, Orkin S. Transcription factor GATA-1 permits survival and maturation of erythroid precursors by preventing apoptosis. Proc Natl Acad Sci U S A. 1995;92:9623-7 pubmed
    ..These results illustrate the multifunctional nature of GATA-1 and suggest a mechanism by which other hematopoietic transcription factors may ensure the development of specific lineages. ..
  7. Vannucchi A, Linari S, Lin C, Koury M, Bondurant M, Migliaccio A. Increased expression of the distal, but not of the proximal, Gata1 transcripts during differentiation of primary erythroid cells. J Cell Physiol. 1999;180:390-401 pubmed
    b>Gata1 is expressed from either one of two alternative promoters, the erythroid (proximal to the AUG) and the testis (distal to the AUG) promoter, both used by hemopoietic cells...
  8. Nishiyama C, Ito T, Nishiyama M, Masaki S, Maeda K, Nakano N, et al. GATA-1 is required for expression of Fc{epsilon}RI on mast cells: analysis of mast cells derived from GATA-1 knockdown mouse bone marrow. Int Immunol. 2005;17:847-56 pubmed
    ..We concluded that the transcription factor GATA-1 positively regulates FcepsilonRI alpha- and beta-chain expression and therefore is involved in mast cell development. ..
  9. Kitajima K, Zheng J, Yen H, Sugiyama D, Nakano T. Multipotential differentiation ability of GATA-1-null erythroid-committed cells. Genes Dev. 2006;20:654-9 pubmed
    ..Our data suggest that GATA-1 is a critical transcription factor to fix erythroid progenitors to the erythroid lineage. ..
  10. Huang Z, Richmond T, Muntean A, Barber D, Weiss M, Crispino J. STAT1 promotes megakaryopoiesis downstream of GATA-1 in mice. J Clin Invest. 2007;117:3890-9 pubmed
    ..We previously reported that STAT1 expression was significantly downregulated in Gata1-knockdown murine megakaryocytes, which also have impaired terminal maturation...
  11. Shimizu R, Engel J, Yamamoto M. GATA1-related leukaemias. Nat Rev Cancer. 2008;8:279-87 pubmed publisher
    b>GATA1 is a prototypical lineage-restricted transcription factor that is central to the correct differentiation, proliferation and apoptosis of erythroid and megakaryocytic cells...
  12. Shivdasani R, Fujiwara Y, McDevitt M, Orkin S. A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development. EMBO J. 1997;16:3965-73 pubmed
    ..These findings reveal a critical role for GATA-1 in megakaryocyte growth regulation and platelet biogenesis, and illustrate how targeted mutation of cis-elements can generate lineage-specific knockout mice. ..
  13. Tsai F, Browne C, Orkin S. Knock-in mutation of transcription factor GATA-3 into the GATA-1 locus: partial rescue of GATA-1 loss of function in erythroid cells. Dev Biol. 1998;196:218-27 pubmed
    ..Our findings suggest that GATA-3 protein is functional when expressed in an erythroid environment and is competent to act on at least a subset of erythroid-expressed target genes in vivo. ..
  14. Zon L, Gurish M, Stevens R, Mather C, Reynolds D, Austen K, et al. GATA-binding transcription factors in mast cells regulate the promoter of the mast cell carboxypeptidase A gene. J Biol Chem. 1991;266:22948-53 pubmed
    ..GATA-1, GATA-2, and GATA-3 are thus the first DNA-binding proteins identified in mast cells which regulate the promoter activity of a gene that encodes a secretory granule protease. ..
  15. Wontakal S, Guo X, Smith C, MacCarthy T, Bresnick E, Bergman A, et al. A core erythroid transcriptional network is repressed by a master regulator of myelo-lymphoid differentiation. Proc Natl Acad Sci U S A. 2012;109:3832-7 pubmed publisher
  16. Ketola I, Anttonen M, Vaskivuo T, Tapanainen J, Toppari J, Heikinheimo M. Developmental expression and spermatogenic stage specificity of transcription factors GATA-1 and GATA-4 and their cofactors FOG-1 and FOG-2 in the mouse testis. Eur J Endocrinol. 2002;147:397-406 pubmed
    ..The findings suggest that, in contrast to the hematopoietic system and the heart, GATA-1 and GATA-4 do not use FOG-1 and FOG-2 respectively as their only cofactors during the early stages of testicular development. ..
  17. Yao X, Kodeboyina S, Liu L, Dzandu J, Sangerman J, Ofori Acquah S, et al. Role of STAT3 and GATA-1 interactions in gamma-globin gene expression. Exp Hematol. 2009;37:889-900 pubmed publisher
    ..Therefore, we completed studies to determine if interactions between these two factors influence gamma-globin gene expression...
  18. Yu Y, Chiang Y, Chen Y, Papetti M, Juo C, Skoultchi A, et al. MAPK-mediated phosphorylation of GATA-1 promotes Bcl-XL expression and cell survival. J Biol Chem. 2005;280:29533-42 pubmed
    ..Therefore, GATA-1 may represent a novel MAPK substrate that plays an essential role in a cytokine-mediated antiapoptotic response. ..
  19. Holmes M, Turner J, Fox A, Chisholm O, Crossley M, Chong B. hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates GATA-mediated activation. J Biol Chem. 1999;274:23491-8 pubmed
    ..Finally, we have identified a repression domain in hFOG-2 and show that repression is dependent upon the integrity of the mCtBP2 interaction motif Pro-Ile-Asp-Leu-Ser. ..
  20. Crawford S, Qi C, Misra P, Stellmach V, Rao M, Engel J, et al. Defects of the heart, eye, and megakaryocytes in peroxisome proliferator activator receptor-binding protein (PBP) null embryos implicate GATA family of transcription factors. J Biol Chem. 2002;277:3585-92 pubmed
    ..These observations identify the GATA family of transcription factors as a new interacting partner of PBP and demonstrate that PBP is essential for normal development of vital organ systems. ..
  21. Ito E, Toki T, Ishihara H, Ohtani H, Gu L, Yokoyama M, et al. Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis. Nature. 1993;362:466-8 pubmed
    ..The GATA-1-expressing cells in 10-week-old testis were found only in contact with the basement membrane of seminiferous tubules, suggesting that GATA-1 regulates genes during the earliest stages of spermatogenesis. ..
  22. Jippo T, Mizuno H, Xu Z, Nomura S, Yamamoto M, Kitamura Y. Abundant expression of transcription factor GATA-2 in proliferating but not in differentiated mast cells in tissues of mice: demonstration by in situ hybridization. Blood. 1996;87:993-8 pubmed
    ..The CMCs stopped both the proliferation and GATA-2 expression after the transplantation, suggesting the association of these two parameters in mast cells within tissues of mice. ..
  23. Yu M, Riva L, Xie H, Schindler Y, Moran T, Cheng Y, et al. Insights into GATA-1-mediated gene activation versus repression via genome-wide chromatin occupancy analysis. Mol Cell. 2009;36:682-95 pubmed publisher
    ..These data provide insights into GATA-1-mediated gene regulation in vivo. ..
  24. Lindeboom F, Gillemans N, Karis A, Jaegle M, Meijer D, Grosveld F, et al. A tissue-specific knockout reveals that Gata1 is not essential for Sertoli cell function in the mouse. Nucleic Acids Res. 2003;31:5405-12 pubmed
    The transcription factor Gata1 is essential for the development of erythroid cells. Consequently, Gata1 null mutants die in utero due to severe anaemia...
  25. Pevny L, Simon M, Robertson E, Klein W, Tsai S, D AGATI V, et al. Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1. Nature. 1991;349:257-60 pubmed
    ..This demonstrates that GATA-1 is required for the normal differentiation of erythroid cells, and that other GATA-binding proteins cannot compensate for its absence. ..
  26. Sugiyama D, Tanaka M, Kitajima K, Zheng J, Yen H, Murotani T, et al. Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation. Blood. 2008;111:1924-32 pubmed
    ..These results indicate that FOG-1 inhibits mast-cell differentiation in a differentiation stage-dependent manner, and its effects are produced via different molecular mechanisms. ..
  27. Shalaby F, Rossant J, Yamaguchi T, Gertsenstein M, Wu X, Breitman M, et al. Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature. 1995;376:62-6 pubmed
    ..These results indicate that Flk-1 is essential for yolk-sac blood-island formation and vasculogenesis in the mouse embryo. ..
  28. Yu C, Cantor A, Yang H, Browne C, Wells R, Fujiwara Y, et al. Targeted deletion of a high-affinity GATA-binding site in the GATA-1 promoter leads to selective loss of the eosinophil lineage in vivo. J Exp Med. 2002;195:1387-95 pubmed
    ..Mice lacking the ability to produce eosinophils should prove useful in ascertaining the role of eosinophils in a variety of inflammatory or allergic disorders. ..
  29. Swartz J, Dyer K, Cheever A, Ramalingam T, Pesnicak L, Domachowske J, et al. Schistosoma mansoni infection in eosinophil lineage-ablated mice. Blood. 2006;108:2420-7 pubmed
    ..However, eosinophils may have unexplored immunomodulatory contributions to this disease process. ..
  30. Miccio A, Wang Y, Hong W, Gregory G, Wang H, Yu X, et al. NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development. EMBO J. 2010;29:442-56 pubmed publisher
    ..Mice in which the FOG-1/NuRD interaction is disrupted display defects similar to germline mutations in the Gata1 and Fog1 genes, including anaemia and macrothrombocytopaenia...
  31. Migliaccio A, Rana R, Sanchez M, Lorenzini R, Centurione L, Bianchi L, et al. GATA-1 as a regulator of mast cell differentiation revealed by the phenotype of the GATA-1low mouse mutant. J Exp Med. 2003;197:281-96 pubmed
    ..These abnormalities, which were mirrored by impaired mast differentiation in vitro, were reversed by retroviral-mediated expression of GATA-1 cDNA. These data indicate an essential role for GATA-1 in mast cell differentiation...
  32. Shimizu R, Takahashi S, Ohneda K, Engel J, Yamamoto M. In vivo requirements for GATA-1 functional domains during primitive and definitive erythropoiesis. EMBO J. 2001;20:5250-60 pubmed
  33. Johnson K, Boyer M, Kang J, Wickrema A, Cantor A, Bresnick E. Friend of GATA-1-independent transcriptional repression: a novel mode of GATA-1 function. Blood. 2007;109:5230-3 pubmed
    ..The identification of genes repressed by GATA-1 independent of FOG-1 defines a novel mode of GATA-1-mediated transcriptional regulation. ..
  34. Trainor C, Omichinski J, Vandergon T, Gronenborn A, Clore G, Felsenfeld G. A palindromic regulatory site within vertebrate GATA-1 promoters requires both zinc fingers of the GATA-1 DNA-binding domain for high-affinity interaction. Mol Cell Biol. 1996;16:2238-47 pubmed
    ..We propose that GATApal is important for positive regulation of GATA-1 expression in erythroid cells. ..
  35. Palis J, Robertson S, Kennedy M, Wall C, Keller G. Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouse. Development. 1999;126:5073-84 pubmed
  36. Li Z, Godinho F, Klusmann J, Garriga Canut M, Yu C, Orkin S. Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1. Nat Genet. 2005;37:613-9 pubmed
    Acquired mutations in the hematopoietic transcription factor GATA binding protein-1 (GATA1) are found in megakaryoblasts from nearly all individuals with Down syndrome with transient myeloproliferative disorder (TMD, also called ..
  37. Yamaguchi Y, Zon L, Ackerman S, Yamamoto M, Suda T. Forced GATA-1 expression in the murine myeloid cell line M1: induction of c-Mpl expression and megakaryocytic/erythroid differentiation. Blood. 1998;91:450-7 pubmed
    ..These findings suggest that the upregulation of c-mpl induced by GATA-1 expression in M1 cells is closely associated with erythroid and megakaryocytic differentiation. ..
  38. Tripic T, Deng W, Cheng Y, Zhang Y, Vakoc C, Gregory G, et al. SCL and associated proteins distinguish active from repressive GATA transcription factor complexes. Blood. 2009;113:2191-201 pubmed publisher
    ..Together, these studies identify the SCL complex as a critical and consistent determinant of positive GATA-1 activity in multiple GATA-1-regulated hematopoietic cell lineages. ..
  39. Rodriguez P, Bonte E, Krijgsveld J, Kolodziej K, Guyot B, Heck A, et al. GATA-1 forms distinct activating and repressive complexes in erythroid cells. EMBO J. 2005;24:2354-66 pubmed
    ..Based on these findings, we suggest a model for the different roles of GATA-1 in erythroid differentiation. ..
  40. Malinge S, Bliss Moreau M, Kirsammer G, Diebold L, Chlon T, Gurbuxani S, et al. Increased dosage of the chromosome 21 ortholog Dyrk1a promotes megakaryoblastic leukemia in a murine model of Down syndrome. J Clin Invest. 2012;122:948-62 pubmed publisher
    ..Acquired mutations in the transcription factor-encoding GATA1 gene are observed in nearly all individuals with DS who are born with transient myeloproliferative disorder (TMD), ..
  41. Welch J, Watts J, Vakoc C, Yao Y, Wang H, Hardison R, et al. Global regulation of erythroid gene expression by transcription factor GATA-1. Blood. 2004;104:3136-47 pubmed
    ..These findings define a regulatory loop for beta globin expression and, more generally, demonstrate how transcriptome analysis can be used to generate testable hypotheses regarding transcriptional networks. ..
  42. Tsang A, Visvader J, Turner C, Fujiwara Y, Yu C, Weiss M, et al. FOG, a multitype zinc finger protein, acts as a cofactor for transcription factor GATA-1 in erythroid and megakaryocytic differentiation. Cell. 1997;90:109-19 pubmed
    ..These findings indicate that FOG acts as a cofactor for GATA-1 and provide a paradigm for the regulation of cell type-specific gene expression by GATA transcription factors. ..
  43. Van Handel B, Montel Hagen A, Sasidharan R, Nakano H, Ferrari R, Boogerd C, et al. Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium. Cell. 2012;150:590-605 pubmed publisher
    ..These results reveal unexpected plasticity in embryonic endothelium such that loss of a single master regulator can induce ectopic cardiomyogenesis from endothelial cells. ..
  44. Maeda K, Nishiyama C, Tokura T, Akizawa Y, Nishiyama M, Ogawa H, et al. Regulation of cell type-specific mouse Fc epsilon RI beta-chain gene expression by GATA-1 via four GATA motifs in the promoter. J Immunol. 2003;170:334-40 pubmed
    ..These results indicate that cell type-specific transcription of mouse beta-chain gene is regulated by GATA-1. ..
  45. Qamar I, Park E, Gong E, Lee H, Lee K. ARR19 (androgen receptor corepressor of 19 kDa), an antisteroidogenic factor, is regulated by GATA-1 in testicular Leydig cells. J Biol Chem. 2009;284:18021-32 pubmed publisher
  46. Gregory G, Raju S, Winandy S, Brown M. Mast cell IL-4 expression is regulated by Ikaros and influences encephalitogenic Th1 responses in EAE. J Clin Invest. 2006;116:1327-36 pubmed
    ..b>GATA1/2 and Ikaros, factors with opposing roles in chromatin remodeling, acted at these sites...
  47. Chang A, Cantor A, Fujiwara Y, Lodish M, Droho S, Crispino J, et al. GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis. Proc Natl Acad Sci U S A. 2002;99:9237-42 pubmed
    ..On the basis of these and previous reports, we infer that GATA factor dependence is a critical aspect of FOG protein function. ..
  48. Tsang A, Fujiwara Y, Hom D, Orkin S. Failure of megakaryopoiesis and arrested erythropoiesis in mice lacking the GATA-1 transcriptional cofactor FOG. Genes Dev. 1998;12:1176-88 pubmed
    ..We speculate that FOG and other FOG-like proteins serve as complex cofactors that act through both GATA-dependent and GATA-independent mechanisms. ..
  49. Ferreira R, Ohneda K, Yamamoto M, Philipsen S. GATA1 function, a paradigm for transcription factors in hematopoiesis. Mol Cell Biol. 2005;25:1215-27 pubmed
  50. Chlon T, Dore L, Crispino J. Cofactor-mediated restriction of GATA-1 chromatin occupancy coordinates lineage-specific gene expression. Mol Cell. 2012;47:608-21 pubmed publisher
    ..We identified GATA1-selective and GATA-1(V205G)-selective binding sites and show that GATA-1, in the absence of FOG-1, occupies GATA-1(..
  51. Letting D, Rakowski C, Weiss M, Blobel G. Formation of a tissue-specific histone acetylation pattern by the hematopoietic transcription factor GATA-1. Mol Cell Biol. 2003;23:1334-40 pubmed
    ..Together, these results suggest that GATA-1 and its cofactor CBP are essential for the formation of an erythroid-specific acetylation pattern that is permissive for high levels of gene expression. ..
  52. Gutierrez L, Tsukamoto S, Suzuki M, Yamamoto Mukai H, Yamamoto M, Philipsen S, et al. Ablation of Gata1 in adult mice results in aplastic crisis, revealing its essential role in steady-state and stress erythropoiesis. Blood. 2008;111:4375-85 pubmed publisher
    The transcription factor Gata1 is expressed in several hematopoietic lineages and plays essential roles in normal hematopoietic development during embryonic stages...
  53. Watamoto K, Towatari M, Ozawa Y, Miyata Y, Okamoto M, Abe A, et al. Altered interaction of HDAC5 with GATA-1 during MEL cell differentiation. Oncogene. 2003;22:9176-84 pubmed
    ..These observations support the suggestion that control of the HDAC5 nucleocytoplasmic distribution might be associated with MEL cell differentiation, possibly through regulated GATA-1 transactivation. ..
  54. Weiss M, Keller G, Orkin S. Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells. Genes Dev. 1994;8:1184-97 pubmed
    ..The approach used here is a paradigm for the phenotypic analysis of targeted mutations affecting hematopoietic development. ..
  55. Rooke H, Orkin S. Phosphorylation of Gata1 at serine residues 72, 142, and 310 is not essential for hematopoiesis in vivo. Blood. 2006;107:3527-30 pubmed
    ..The indispensable zinc-finger transcription factor, Gata1, is phosphorylated constitutively at 6 serine residues (26, 49, 72, 142, 178, 187), and at a seventh (310) ..
  56. McDevitt M, Shivdasani R, Fujiwara Y, Yang H, Orkin S. A "knockdown" mutation created by cis-element gene targeting reveals the dependence of erythroid cell maturation on the level of transcription factor GATA-1. Proc Natl Acad Sci U S A. 1997;94:6781-5 pubmed
    ..Our work illustrates the usefulness of targeted mutations to create knockdown mutations that may uncover important quantitative contributions of gene function not revealed by conventional knockouts. ..
  57. Simon M, Pevny L, Wiles M, Keller G, Costantini F, Orkin S. Rescue of erythroid development in gene targeted GATA-1- mouse embryonic stem cells. Nat Genet. 1992;1:92-8 pubmed
    ..Use of in vitro differentiated ES cells bridges a gap between conventional approaches to gene function in cell lines and analysis of loss of function mutations in the whole animal. ..
  58. Valverde Garduno V, Guyot B, Anguita E, Hamlett I, Porcher C, Vyas P. Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 loci: implications for cis-element identification. Blood. 2004;104:3106-16 pubmed
    ..Human and mouse GATA1 genes encode a critical hematopoietic transcription factor conserved in expression and function...
  59. Tsai S, Martin D, Zon L, D Andrea A, Wong G, Orkin S. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells. Nature. 1989;339:446-51 pubmed
    ..The factor, a new member of the zinc-finger family of DNA-binding proteins, is restricted to erythroid cells at the level of RNA expression and is closely homologous between mouse and man. ..
  60. Harju Baker S, Costa F, Fedosyuk H, Neades R, Peterson K. Silencing of Agamma-globin gene expression during adult definitive erythropoiesis mediated by GATA-1-FOG-1-Mi2 complex binding at the -566 GATA site. Mol Cell Biol. 2008;28:3101-13 pubmed publisher
  61. Wu W, Cheng Y, Keller C, Ernst J, Kumar S, Mishra T, et al. Dynamics of the epigenetic landscape during erythroid differentiation after GATA1 restoration. Genome Res. 2011;21:1659-71 pubmed publisher
    ..genome-wide during mouse erythroid differentiation dependent on the master regulatory transcription factor GATA1. Notably, despite extensive changes in gene expression, the chromatin state profiles (proportions of a gene in a ..
  62. Kataoka H, Hayashi M, Nakagawa R, Tanaka Y, Izumi N, Nishikawa S, et al. Etv2/ER71 induces vascular mesoderm from Flk1+PDGFR?+ primitive mesoderm. Blood. 2011;118:6975-86 pubmed publisher
    ..We propose that Flk-1(+)/PDGFR?(+) primitive mesoderm is committed into Flk-1(+)/PDGFR?(-) vascular mesoderm through Etv2 and that up-regulation of Etv2 by VEGF promotes this commitment...
  63. Barrionuevo F, Georg I, Scherthan H, Lecureuil C, Guillou F, Wegner M, et al. Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8. Dev Biol. 2009;327:301-12 pubmed publisher
    ..This study shows that testis cord differentiation is independent of Sox9, and that concerted Sox9 and Sox8 function in post E14.0 Sertoli cells is essential for the maintenance of testicular function. ..
  64. Chu V, Beller A, Rausch S, Strandmark J, Zänker M, Arbach O, et al. Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis. Immunity. 2014;40:582-93 pubmed publisher
    ..Our findings show that eosinophils are important players for immune homeostasis in gut-associated tissues and add to data suggesting that eosinophils can promote tissue integrity...
  65. Takahashi S, Onodera K, Motohashi H, Suwabe N, Hayashi N, Yanai N, et al. Arrest in primitive erythroid cell development caused by promoter-specific disruption of the GATA-1 gene. J Biol Chem. 1997;272:12611-5 pubmed
  66. Suzuki M, Ohneda K, Hosoya Ohmura S, Tsukamoto S, Ohneda O, Philipsen S, et al. Real-time monitoring of stress erythropoiesis in vivo using Gata1 and beta-globin LCR luciferase transgenic mice. Blood. 2006;108:726-33 pubmed
    ..system for erythropoiesis using transgenic mouse lines expressing luciferase under the control of the mouse Gata1 hematopoietic regulatory domain (G1-HRD-luc) or human beta-globin locus control region (Hbb-LCR-luc)...
  67. Rylski M, Welch J, Chen Y, Letting D, Diehl J, Chodosh L, et al. GATA-1-mediated proliferation arrest during erythroid maturation. Mol Cell Biol. 2003;23:5031-42 pubmed
    ..Our results illustrate how GATA-1, a lineage-determining transcription factor, coordinates proliferation arrest with cellular maturation through distinct, interrelated genetic programs. ..
  68. Ohmori S, Takai J, Ishijima Y, Suzuki M, Moriguchi T, Philipsen S, et al. Regulation of GATA factor expression is distinct between erythroid and mast cell lineages. Mol Cell Biol. 2012;32:4742-55 pubmed publisher
    The zinc finger transcription factors GATA1 and GATA2 participate in mast cell development...
  69. Fulkerson P, Fischetti C, McBride M, Hassman L, Hogan S, Rothenberg M. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc Natl Acad Sci U S A. 2006;103:16418-23 pubmed
    ..In summary, we present multiple lines of independent evidence that eosinophils via CCR3 have a central role in chronic allergic airway disease. ..
  70. Voehringer D, Reese T, Huang X, Shinkai K, Locksley R. Type 2 immunity is controlled by IL-4/IL-13 expression in hematopoietic non-eosinophil cells of the innate immune system. J Exp Med. 2006;203:1435-46 pubmed
    ..Thus, components of type 2 immunity mediated by IL-4/IL-13 are partitioned between T cell-dependent IgE and an innate non-eosinophil tissue component, suggesting new strategies for interventions in allergic immunity. ..
  71. Elagib K, Mihaylov I, Delehanty L, Bullock G, Ouma K, Caronia J, et al. Cross-talk of GATA-1 and P-TEFb in megakaryocyte differentiation. Blood. 2008;112:4884-94 pubmed publisher
    ..Our results offer evidence for P-TEFb cross-talk with GATA-1 in megakaryocytic differentiation, a program with parallels to cardiomyocyte hypertrophy. ..
  72. Humbles A, Lloyd C, McMillan S, Friend D, Xanthou G, McKenna E, et al. A critical role for eosinophils in allergic airways remodeling. Science. 2004;305:1776-9 pubmed
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    ..These findings provide the first example of a chain of coupling mechanisms between the binding of erythropoietin to its receptor and GATA-1-dependent gene expression. ..
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    ..Direct interaction of the RBTN2 protein was observed in vivo and in vitro with the GATA1 or -2 zinc-finger transcription factors, as well as with the basic helix-loop-helix protein TAL1...
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    ..Together, these data demonstrate that GATA-1 and FOG-1 are essential anchors for a tissue-specific chromatin loop, providing general insights into long-range enhancer function. ..
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    ..These results reveal a miRNA locus that is required for erythropoiesis and uncover a new regulatory axis through which GATA-1 controls this process. ..
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