Gene Symbol: Gal3st1
Description: galactose-3-O-sulfotransferase 1
Alias: Cst, Gcst, galactosylceramide sulfotransferase, 3'-phosphoadenosine-5'-phosphosulfate:Galcer sulfotransferase, 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase, Galcer sulfotransferase, cerebroside sulfotransferase
Species: mouse
Products:     Gal3st1

Top Publications

  1. Schafer D, Bansal R, Hedstrom K, Pfeiffer S, Rasband M. Does paranode formation and maintenance require partitioning of neurofascin 155 into lipid rafts?. J Neurosci. 2004;24:3176-85 pubmed
    ..Together, these results suggest that trans interactions between oligodendroglial NF-155 and axonal ligands result in cross-linking, stabilization, and formation of paranodal lipid raft assemblies. ..
  2. Ishibashi T, Dupree J, Ikenaka K, Hirahara Y, Honke K, Peles E, et al. A myelin galactolipid, sulfatide, is essential for maintenance of ion channels on myelinated axon but not essential for initial cluster formation. J Neurosci. 2002;22:6507-14 pubmed
    ..distribution of axonal components in mice incapable of synthesizing sulfatide by disruption of the galactosylceramide sulfotransferase gene...
  3. Shroff S, Pomicter A, Chow W, Fox M, Colello R, Henderson S, et al. Adult CST-null mice maintain an increased number of oligodendrocytes. J Neurosci Res. 2009;87:3403-14 pubmed publisher
    ..We have also reported that mice incapable of synthesizing sulfatide (CST-null) while maintaining normal levels of galactocerebroside generate relatively stable myelin with unstable ..
  4. Marcus J, Honigbaum S, Shroff S, Honke K, Rosenbluth J, Dupree J. Sulfatide is essential for the maintenance of CNS myelin and axon structure. Glia. 2006;53:372-81 pubmed
  5. Honke K, Hirahara Y, Dupree J, Suzuki K, Popko B, Fukushima K, et al. Paranodal junction formation and spermatogenesis require sulfoglycolipids. Proc Natl Acad Sci U S A. 2002;99:4227-32 pubmed
    ..Both sulfoglycolipids can be synthesized in vitro by using the isolated cerebroside sulfotransferase. To investigate the physiological role of sulfoglycolipids and to determine whether sulfatide and ..
  6. Hirahara Y, Bansal R, Honke K, Ikenaka K, Wada Y. Sulfatide is a negative regulator of oligodendrocyte differentiation: development in sulfatide-null mice. Glia. 2004;45:269-77 pubmed
    ..In the present study, we examined oligodendrocytes differentiation in cerebroside sulfotransferase-null mice (Cst(-/-)) that lack sulfatide but express GalC...
  7. Hoshi T, Suzuki A, Hayashi S, Tohyama K, Hayashi A, Yamaguchi Y, et al. Nodal protrusions, increased Schmidt-Lanterman incisures, and paranodal disorganization are characteristic features of sulfatide-deficient peripheral nerves. Glia. 2007;55:584-94 pubmed
    ..All of these regions are involved in axo-glial interactions. In the present study, we examined cerebroside sulfotransferase-deficient mice to determine whether sulfatide is essential for axo-glial interactions in these PNS ..
  8. Wang C, Wang M, Zhou Y, Dupree J, Han X. Alterations in mouse brain lipidome after disruption of CST gene: a lipidomics study. Mol Neurobiol. 2014;50:88-96 pubmed publisher
    To investigate the effects of a critical enzyme, cerebroside sulfotransferase (CST), involving sulfatide biosynthesis on lipid (particularly sphingolipid) homeostasis, herein, we determined the lipidomes of brain cortex and spinal cord ..
  9. Hirahara Y, Wakabayashi T, Mori T, Koike T, Yao I, Tsuda M, et al. Sulfatide species with various fatty acid chains in oligodendrocytes at different developmental stages determined by imaging mass spectrometry. J Neurochem. 2017;140:435-450 pubmed publisher
    ..Therefore, individual sulfatide species may have unique roles in oligodendrocyte maturation and myelination. Read the Editorial Highlight for this article on page 356. ..

More Information


  1. Kondo Y, Tokuda N, Fan X, Yamashita T, Honke K, Takematsu H, et al. Glycosphingolipids are not pivotal receptors for Subtilase cytotoxin in vivo: sensitivity analysis with glycosylation-defective mutant mice. Biochem Biophys Res Commun. 2009;378:179-81 pubmed publisher
    ..5 microg) of SubAB as well as wild type mice. These results indicated none of glycolipids are not pivotal receptor for SubAB in the body. ..
  2. Yaghootfam A, Sorkalla T, Häberlein H, Gieselmann V, Kappler J, Eckhardt M. Cerebroside sulfotransferase forms homodimers in living cells. Biochemistry. 2007;46:9260-9 pubmed
    b>Cerebroside sulfotransferase (CST) catalyzes the 3'-sulfation of galactose residues in several glycolipids. Its major product in the mammalian brain is sulfatide, which is an essential myelin component...
  3. Suzuki A, Hoshi T, Ishibashi T, Hayashi A, Yamaguchi Y, Baba H. Paranodal axoglial junction is required for the maintenance of the Nav1.6-type sodium channel in the node of Ranvier in the optic nerves but not in peripheral nerve fibers in the sulfatide-deficient mice. Glia. 2004;46:274-83 pubmed
    ..Thus, the paranodal junction significantly influences the retention of Nav1.6 in the node, which is followed by disorganization of nodal structures. However, its importance may differ between the central and peripheral nervous system. ..
  4. Takano M, Hikishima K, Fujiyoshi K, Shibata S, Yasuda A, Konomi T, et al. MRI characterization of paranodal junction failure and related spinal cord changes in mice. PLoS ONE. 2012;7:e52904 pubmed publisher
    ..whether MRI and DTI could detect structural changes in the paranodal junctions of the spinal cord in cerebroside sulfotransferase knock-out (CST-KO) mice...
  5. Hirahara Y, Tsuda M, Wada Y, Honke K. cDNA cloning, genomic cloning, and tissue-specific regulation of mouse cerebroside sulfotransferase. Eur J Biochem. 2000;267:1909-17 pubmed
    ..isolated a mouse cDNA clone encoding 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase (cerebroside sulfotransferase; CST; EC from a kidney cDNA library, using a human CST cDNA clone [Honke, K., Tsuda, M...
  6. Suzuki T, Kosaka Suzuki N, Pack S, Shin D, Yoon J, Abdullaev Z, et al. Expression of a testis-specific form of Gal3st1 (CST), a gene essential for spermatogenesis, is regulated by the CTCF paralogous gene BORIS. Mol Cell Biol. 2010;30:2473-84 pubmed publisher
    ..By gene expression profiling, we found that transcript levels for Gal3st1 (also known as cerebroside sulfotransferase [CST]), known to play a crucial role in meiosis, were dramatically reduced in BORIS KO testes...
  7. Hayashi A, Kaneko N, Tomihira C, Baba H. Sulfatide decrease in myelin influences formation of the paranodal axo-glial junction and conduction velocity in the sciatic nerve. Glia. 2013;61:466-74 pubmed publisher
    b>Cerebroside sulfotransferase (CST) catalyzes the production of sulfatide, which is one of the major glycolipids in myelin. Homozygous CST knockout mice were shown to be completely deficient in sulfatide...
  8. Marsching C, Jennemann R, Heilig R, Gröne H, Hopf C, Sandhoff R. Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods. J Lipid Res. 2014;55:2343-53 pubmed publisher
    ..In conclusion, our study suggests that MALDI IMS is useful for semi-quantitative dissection of relative local changes of sulfatides and possibly other lipids in tissue. ..
  9. Stettner P, Bourgeois S, Marsching C, Traykova Brauch M, Porubsky S, Nordström V, et al. Sulfatides are required for renal adaptation to chronic metabolic acidosis. Proc Natl Acad Sci U S A. 2013;110:9998-10003 pubmed publisher
    ..This study points to a seminal role of sulfatides in renal ammonium handling, urinary acidification, and acid-base homeostasis. ..
  10. Zhang Y, Hayashi Y, Cheng X, Watanabe T, Wang X, Taniguchi N, et al. Testis-specific sulfoglycolipid, seminolipid, is essential for germ cell function in spermatogenesis. Glycobiology. 2005;15:649-54 pubmed
    ..The sulfation of the molecule is catalyzed by a Golgi membrane-associated sulfotransferase, cerebroside sulfotransferase (CST)...
  11. Kajigaya H, Tanaka K, Hayashi A, Suzuki A, Ishibashi T, Ikenaka K, et al. Increased numbers of oligodendrocyte lineage cells in the optic nerves of cerebroside sulfotransferase knockout mice. Proc Jpn Acad Ser B Phys Biol Sci. 2011;87:415-24 pubmed
    ..b>Cerebroside sulfotransferase (CST) catalyzes the production of two sulfated glycolipids, sulfatide and proligodendroblast antigen, ..
  12. Fewou S, Fernandes A, Stockdale K, Francone V, Dupree J, Rosenbluth J, et al. Myelin protein composition is altered in mice lacking either sulfated or both sulfated and non-sulfated galactolipids. J Neurochem. 2010;112:599-610 pubmed publisher
    ..Mice, unable to synthesize GalCer and sulfatide (CGT(null)) or sulfatide alone (CST(null)), exhibit disorganized paranodal structures and progressive dysmyelination...
  13. Ishibashi T, Ding L, Ikenaka K, Inoue Y, Miyado K, Mekada E, et al. Tetraspanin protein CD9 is a novel paranodal component regulating paranodal junctional formation. J Neurosci. 2004;24:96-102 pubmed
    ..myelination proceeds, but CD9 clusters become diffuse, associated with disruption of the paranode, in cerebroside sulfotransferase-deficient mice...
  14. von Gerichten J, Schlosser K, Lamprecht D, Morace I, Eckhardt M, Wachten D, et al. Diastereomer-specific quantification of bioactive hexosylceramides from bacteria and mammals. J Lipid Res. 2017;58:1247-1258 pubmed publisher
    ..Vice versa, ?-GalCer increased specifically when cerebroside sulfotransferase (Gal3st1) was deleted...
  15. Sheng X, Nakajima T, Wang L, Zhang X, Kamijo Y, Takahashi K, et al. Attenuation of kidney injuries maintains serum sulfatide levels dependent on hepatic synthetic ability: a possible involvement of oxidative stress. Tohoku J Exp Med. 2012;227:1-12 pubmed
    ..This was the result of decreased hepatic expression of a sulfatide synthetic enzyme, cerebroside sulfotransferase (CST), which is associated with systemic enhancement of oxidative stress...
  16. Mierzwa A, Arevalo J, Schiff R, Chao M, Rosenbluth J. Role of transverse bands in maintaining paranodal structure and axolemmal domain organization in myelinated nerve fibers: effect on longevity in dysmyelinated mutant mice. J Comp Neurol. 2010;518:2841-53 pubmed publisher we compare this mutant with other dysmyelinated mutants including the ceramide sulfotransferase deficient (CST-/-) mouse, which are more severely affected behaviorally, to determine what might underlie the differences between ..
  17. Blomqvist M, Rhost S, Teneberg S, Löfbom L, Osterbye T, Brigl M, et al. Multiple tissue-specific isoforms of sulfatide activate CD1d-restricted type II NKT cells. Eur J Immunol. 2009;39:1726-35 pubmed publisher