Ehmt1

Summary

Gene Symbol: Ehmt1
Description: euchromatic histone methyltransferase 1
Alias: 9230102N17Rik, D330003E03, Eu-HMTase1, GLP, GLP1, KMT1D, mKIAA1876, histone-lysine N-methyltransferase EHMT1, G9a-like protein 1, euchromatic histone-lysine N-methyltransferase 1, lysine N-methyltransferase 1D
Species: mouse
Products:     Ehmt1

Top Publications

  1. Tachibana M, Ueda J, Fukuda M, Takeda N, Ohta T, Iwanari H, et al. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Genes Dev. 2005;19:815-26 pubmed
    ..There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1. Here we show that GLP is also important for H3-K9 methylation of mouse euchromatin...
  2. Schaefer A, Sampath S, Intrator A, Min A, Gertler T, Surmeier D, et al. Control of cognition and adaptive behavior by the GLP/G9a epigenetic suppressor complex. Neuron. 2009;64:678-91 pubmed publisher
    ..symptoms of the human 9q34 mental retardation syndrome that is associated with structural alterations of the GLP/EHMT1 gene...
  3. Tachibana M, Matsumura Y, Fukuda M, Kimura H, Shinkai Y. G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. EMBO J. 2008;27:2681-90 pubmed publisher
    ..G9a and GLP/Eu-HMTase1 are two-related mammalian lysine methyltransferases and a G9a/GLP heteromeric complex regulates H3K9 ..
  4. Yamaji M, Seki Y, Kurimoto K, Yabuta Y, Yuasa M, Shigeta M, et al. Critical function of Prdm14 for the establishment of the germ cell lineage in mice. Nat Genet. 2008;40:1016-22 pubmed publisher
    ..Our combined evidence demonstrates that Prdm14 defines a previously unknown genetic pathway, initiating independently from Prdm1, for ensuring the launching of the mammalian germ cell lineage. ..
  5. Tachibana M, Nozaki M, Takeda N, Shinkai Y. Functional dynamics of H3K9 methylation during meiotic prophase progression. EMBO J. 2007;26:3346-59 pubmed
    ..This genetic and biochemical evidence strongly suggests that a specific set of H3K9 methyltransferase(s) and demethylase(s) coordinately regulate gametogenesis. ..
  6. Han P, Li W, Yang J, Shang C, Lin C, Cheng W, et al. Epigenetic response to environmental stress: Assembly of BRG1-G9a/GLP-DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts. Biochim Biophys Acta. 2016;1863:1772-81 pubmed publisher
    ..In mice, pathological stress activates cardiomyocytes to express Brg1 (nucleosome-remodeling factor), G9a/Glp (histone methyltransferase), and Dnmt3 (DNA methyltransferase)...
  7. Chang Y, Sun L, Kokura K, Horton J, Fukuda M, Espejo A, et al. MPP8 mediates the interactions between DNA methyltransferase Dnmt3a and H3K9 methyltransferase GLP/G9a. Nat Commun. 2011;2:533 pubmed publisher
    ..Here we show that G9a or G9a-like protein (GLP) dimethylate the amino-terminal lysine 44 (K44) of mouse Dnmt3a (equivalent to K47 of human DNMT3A) in vitro and in ..
  8. Hasegawa Y, Ikeda K, Chen Y, Alba D, Stifler D, Shinoda K, et al. Repression of Adipose Tissue Fibrosis through a PRDM16-GTF2IRD1 Complex Improves Systemic Glucose Homeostasis. Cell Metab. 2018;27:180-194.e6 pubmed publisher
    ..the transcription of transforming growth factor β-dependent pro-fibrosis genes by recruiting PRDM16 and EHMT1 onto their promoter/enhancer regions...
  9. Thomas L, Miyashita H, Cobb R, Pierce S, Tachibana M, Hobeika E, et al. Functional analysis of histone methyltransferase g9a in B and T lymphocytes. J Immunol. 2008;181:485-93 pubmed
    ..These findings indicate that the H3K9me2 epigenetic mark affects a highly restricted set of processes during lymphocyte development and activation. ..
  10. Shirato H, Ogawa S, Nakajima K, Inagawa M, Kojima M, Tachibana M, et al. A jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9. J Biol Chem. 2009;284:733-9 pubmed publisher
    ..We also found that Jmj bound to the H3-K9 methyltransferases G9a and GLP. Expression of Jmj recruited G9a and GLP to the cyclin D1 promoter and increased H3-K9 methylation...

Detail Information

Publications40

  1. Tachibana M, Ueda J, Fukuda M, Takeda N, Ohta T, Iwanari H, et al. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Genes Dev. 2005;19:815-26 pubmed
    ..There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1. Here we show that GLP is also important for H3-K9 methylation of mouse euchromatin...
  2. Schaefer A, Sampath S, Intrator A, Min A, Gertler T, Surmeier D, et al. Control of cognition and adaptive behavior by the GLP/G9a epigenetic suppressor complex. Neuron. 2009;64:678-91 pubmed publisher
    ..symptoms of the human 9q34 mental retardation syndrome that is associated with structural alterations of the GLP/EHMT1 gene...
  3. Tachibana M, Matsumura Y, Fukuda M, Kimura H, Shinkai Y. G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. EMBO J. 2008;27:2681-90 pubmed publisher
    ..G9a and GLP/Eu-HMTase1 are two-related mammalian lysine methyltransferases and a G9a/GLP heteromeric complex regulates H3K9 ..
  4. Yamaji M, Seki Y, Kurimoto K, Yabuta Y, Yuasa M, Shigeta M, et al. Critical function of Prdm14 for the establishment of the germ cell lineage in mice. Nat Genet. 2008;40:1016-22 pubmed publisher
    ..Our combined evidence demonstrates that Prdm14 defines a previously unknown genetic pathway, initiating independently from Prdm1, for ensuring the launching of the mammalian germ cell lineage. ..
  5. Tachibana M, Nozaki M, Takeda N, Shinkai Y. Functional dynamics of H3K9 methylation during meiotic prophase progression. EMBO J. 2007;26:3346-59 pubmed
    ..This genetic and biochemical evidence strongly suggests that a specific set of H3K9 methyltransferase(s) and demethylase(s) coordinately regulate gametogenesis. ..
  6. Han P, Li W, Yang J, Shang C, Lin C, Cheng W, et al. Epigenetic response to environmental stress: Assembly of BRG1-G9a/GLP-DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts. Biochim Biophys Acta. 2016;1863:1772-81 pubmed publisher
    ..In mice, pathological stress activates cardiomyocytes to express Brg1 (nucleosome-remodeling factor), G9a/Glp (histone methyltransferase), and Dnmt3 (DNA methyltransferase)...
  7. Chang Y, Sun L, Kokura K, Horton J, Fukuda M, Espejo A, et al. MPP8 mediates the interactions between DNA methyltransferase Dnmt3a and H3K9 methyltransferase GLP/G9a. Nat Commun. 2011;2:533 pubmed publisher
    ..Here we show that G9a or G9a-like protein (GLP) dimethylate the amino-terminal lysine 44 (K44) of mouse Dnmt3a (equivalent to K47 of human DNMT3A) in vitro and in ..
  8. Hasegawa Y, Ikeda K, Chen Y, Alba D, Stifler D, Shinoda K, et al. Repression of Adipose Tissue Fibrosis through a PRDM16-GTF2IRD1 Complex Improves Systemic Glucose Homeostasis. Cell Metab. 2018;27:180-194.e6 pubmed publisher
    ..the transcription of transforming growth factor β-dependent pro-fibrosis genes by recruiting PRDM16 and EHMT1 onto their promoter/enhancer regions...
  9. Thomas L, Miyashita H, Cobb R, Pierce S, Tachibana M, Hobeika E, et al. Functional analysis of histone methyltransferase g9a in B and T lymphocytes. J Immunol. 2008;181:485-93 pubmed
    ..These findings indicate that the H3K9me2 epigenetic mark affects a highly restricted set of processes during lymphocyte development and activation. ..
  10. Shirato H, Ogawa S, Nakajima K, Inagawa M, Kojima M, Tachibana M, et al. A jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9. J Biol Chem. 2009;284:733-9 pubmed publisher
    ..We also found that Jmj bound to the H3-K9 methyltransferases G9a and GLP. Expression of Jmj recruited G9a and GLP to the cyclin D1 promoter and increased H3-K9 methylation...
  11. Dan J, Liu Y, Liu N, Chiourea M, Okuka M, Wu T, et al. Rif1 maintains telomere length homeostasis of ESCs by mediating heterochromatin silencing. Dev Cell. 2014;29:7-19 pubmed publisher
    ..Mechanistically, Rif1 interacts and stabilizes H3K9 methylation complex. Thus, Rif1 regulates telomere length homeostasis of ESCs by mediating heterochromatic silencing. ..
  12. Mozzetta C, Pontis J, Fritsch L, Robin P, Portoso M, Proux C, et al. The histone H3 lysine 9 methyltransferases G9a and GLP regulate polycomb repressive complex 2-mediated gene silencing. Mol Cell. 2014;53:277-89 pubmed publisher
    G9a/GLP and Polycomb Repressive Complex 2 (PRC2) are two major epigenetic silencing machineries, which in particular methylate histone H3 on lysines 9 and 27 (H3K9 and H3K27), respectively...
  13. Yoo S, You S, Yoon H, Kim D, Kim H, Lee K, et al. A novel pathogenic role of the ER chaperone GRP78/BiP in rheumatoid arthritis. J Exp Med. 2012;209:871-86 pubmed publisher
    ..In summary, this study shows that the ER chaperone GRP78 is crucial for synoviocyte proliferation and angiogenesis, the pathological hallmark of RA. ..
  14. Martens M, Frega M, Classen J, Epping L, Bijvank E, Benevento M, et al. Euchromatin histone methyltransferase 1 regulates cortical neuronal network development. Sci Rep. 2016;6:35756 pubmed publisher
    Heterozygous mutations or deletions in the human Euchromatin histone methyltransferase 1 (EHMT1) gene cause Kleefstra syndrome, a neurodevelopmental disorder that is characterized by autistic-like features and severe intellectual ..
  15. Tu S, Narendra V, Yamaji M, Vidal S, Rojas L, Wang X, et al. Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016;534:387-90 pubmed publisher
    ..Thus, in contrast to the traditional 'passenger' role of a co-repressor, CBFA2T2 functions synergistically with transcription factors at the crossroads of the fundamental developmental plasticity between uni- and pluripotency. ..
  16. Deguchi K, Nagamatsu G, Miyachi H, Kato Y, Morita S, Kimura H, et al. Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male mouse germ cells. Biol Reprod. 2013;88:36 pubmed publisher
    ..mark of epigenetic silencing, and the expression of two lysine methyltransferases, G9a/Ehmt2/KMT1C and GLP/Ehmt1/KMT1D, in murine male embryonic germ cells after sex determination...
  17. Ohno H, Shinoda K, Ohyama K, Sharp L, Kajimura S. EHMT1 controls brown adipose cell fate and thermogenesis through the PRDM16 complex. Nature. 2013;504:163-7 pubmed publisher
    ..Here we show that euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is an essential BAT-enriched lysine methyltransferase in the PRDM16 transcriptional complex and controls brown ..
  18. Han Y, Zheng Z, Zuo Z, Yu Y, Chen R, Tseng G, et al. Metallothionein 1 h tumour suppressor activity in prostate cancer is mediated by euchromatin methyltransferase 1. J Pathol. 2013;230:184-93 pubmed publisher
    ..MT1h was found to interact with euchromatin histone methyltransferase 1 (EHMT1) and enhanced its methyltransferase activity on histone 3...
  19. Myant K, Termanis A, Sundaram A, Boe T, Li C, Merusi C, et al. LSH and G9a/GLP complex are required for developmentally programmed DNA methylation. Genome Res. 2011;21:83-94 pubmed publisher
    ..methylation patterns and stable gene silencing at specific promoters require cooperation between LSH and the G9a/GLP complex of histone methylases. At such loci, G9a recruitment is compromised when LSH is absent or greatly reduced...
  20. Kuroki S, Okashita N, Baba S, Maeda R, Miyawaki S, Yano M, et al. Rescuing the aberrant sex development of H3K9 demethylase Jmjd1a-deficient mice by modulating H3K9 methylation balance. PLoS Genet. 2017;13:e1007034 pubmed publisher
    ..Here we identified the GLP/G9a H3K9 methyltransferase complex as the enzyme catalyzing H3K9 methylation at the Sry locus...
  21. Ideno H, Shimada A, Imaizumi K, Kimura H, Abe M, Nakashima K, et al. Predominant expression of H3K9 methyltransferases in prehypertrophic and hypertrophic chondrocytes during mouse growth plate cartilage development. Gene Expr Patterns. 2013;13:84-90 pubmed publisher
    ..euchromatic histone lysine N-methyltransferase 2, Ehmt2), GLP (euchromatic histone lysine N-methyltransferase 1, Ehmt1), SETDB1 (SET domain, bifurcated 1), PRDM2 (PR domain containing 2), SUV39H1 (suppressor of variegation 3-9 ..
  22. Trojer P, Zhang J, Yonezawa M, Schmidt A, Zheng H, Jenuwein T, et al. Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins. J Biol Chem. 2009;284:8395-405 pubmed publisher
    ..We also identify members of the JMJD2/KDM4 subfamily of jumonji-C type histone demethylases as being responsible for the removal of H1.4K26 methylation. ..
  23. Chen L, Li Z, Zwolinska A, Smith M, Cross B, Koomen J, et al. MDM2 recruitment of lysine methyltransferases regulates p53 transcriptional output. EMBO J. 2010;29:2538-52 pubmed publisher
    ..The histone methyltransferases SUV39H1 and EHMT1 bind specifically to MDM2 but not to its homolog MDMX...
  24. Balemans M, Ansar M, Oudakker A, van Caam A, Bakker B, Vitters E, et al. Reduced Euchromatin histone methyltransferase 1 causes developmental delay, hypotonia, and cranial abnormalities associated with increased bone gene expression in Kleefstra syndrome mice. Dev Biol. 2014;386:395-407 pubmed publisher
    Haploinsufficiency of Euchromatin histone methyltransferase 1 (EHMT1), a chromatin modifying enzyme, is the cause of Kleefstra syndrome (KS)...
  25. Liu N, Zhang Z, Wu H, Jiang Y, Meng L, Xiong J, et al. Recognition of H3K9 methylation by GLP is required for efficient establishment of H3K9 methylation, rapid target gene repression, and mouse viability. Genes Dev. 2015;29:379-93 pubmed publisher
    b>GLP and G9a are major H3K9 dimethylases and are essential for mouse early embryonic development. GLP and G9a both harbor ankyrin repeat domains that are capable of binding H3K9 methylation...
  26. Balemans M, Kasri N, Kopanitsa M, Afinowi N, Ramakers G, Peters T, et al. Hippocampal dysfunction in the Euchromatin histone methyltransferase 1 heterozygous knockout mouse model for Kleefstra syndrome. Hum Mol Genet. 2013;22:852-66 pubmed publisher
    Euchromatin histone methyltransferase 1 (EHMT1) is a highly conserved protein that catalyzes mono- and dimethylation of histone H3 lysine 9, thereby epigenetically regulating transcription...
  27. Thienpont B, Aronsen J, Robinson E, Okkenhaug H, Loche E, Ferrini A, et al. The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy. J Clin Invest. 2017;127:335-348 pubmed publisher
    ..H3K9me2 was reduced following a miR-217-mediated decrease in expression of the H3K9 dimethyltransferases EHMT1 and EHMT2 (EHMT1/2)...
  28. Zylicz J, Dietmann S, Günesdogan U, Hackett J, Cougot D, Lee C, et al. Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development. elife. 2015;4: pubmed publisher
    ..This epigenetic mechanism is important for priming gene regulatory networks for critical cell fate decisions in rapidly proliferating postimplantation epiblast cells. ..
  29. Renneville A, van Galen P, Canver M, McConkey M, Krill Burger J, Dorfman D, et al. EHMT1 and EHMT2 inhibition induces fetal hemoglobin expression. Blood. 2015;126:1930-9 pubmed publisher
    ..Using a small-molecule screen, we found that UNC0638, a selective inhibitor of EHMT1 and EHMT2 histone methyltransferases, induces γ-globin expression...
  30. Levy D, Kuo A, Chang Y, Schaefer U, Kitson C, Cheung P, et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol. 2011;12:29-36 pubmed publisher
    ..RelAK310me1 was recognized by the ankryin repeat of the histone methyltransferase GLP, which under basal conditions promoted a repressed chromatin state at RelA target genes through GLP-mediated ..
  31. Holt J, Pye V, Boon E, Stewart J, Garcia Higuera I, Moreno S, et al. The APC/C activator FZR1 is essential for meiotic prophase I in mice. Development. 2014;141:1354-65 pubmed publisher
  32. Pirouz M, Pilarski S, Kessel M. A critical function of Mad2l2 in primordial germ cell development of mice. PLoS Genet. 2013;9:e1003712 pubmed publisher
    ..of transfected fibroblasts we found that the interaction of Mad2l2 with the histone methyltransferases G9a and GLP lead to a downregulation of H3K9me2...
  33. Sridharan R, Gonzales Cope M, Chronis C, Bonora G, McKee R, Huang C, et al. Proteomic and genomic approaches reveal critical functions of H3K9 methylation and heterochromatin protein-1? in reprogramming to pluripotency. Nat Cell Biol. 2013;15:872-82 pubmed publisher
    ..Dissecting the contribution of H3K9 methylation to reprogramming, we show that the H3K9 methyltransferases Ehmt1, Ehmt2 and Setdb1 regulate global H3K9me2/me3 levels and that their depletion increases iPSC formation from both ..
  34. Sangle G, Lauffer L, Grieco A, Trivedi S, Iakoubov R, Brubaker P. Novel biological action of the dipeptidylpeptidase-IV inhibitor, sitagliptin, as a glucagon-like peptide-1 secretagogue. Endocrinology. 2012;153:564-73 pubmed publisher
    Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted into the circulation by the intestinal L cell...
  35. Benevento M, Iacono G, Selten M, Ba W, Oudakker A, Frega M, et al. Histone Methylation by the Kleefstra Syndrome Protein EHMT1 Mediates Homeostatic Synaptic Scaling. Neuron. 2016;91:341-55 pubmed publisher
    ..We show that the Kleefstra-syndrome-associated protein EHMT1 plays a critical and cell-autonomous role in synaptic scaling by responding to attenuated neuronal firing or ..
  36. Ea C, Hao S, Yeo K, Baltimore D. EHMT1 protein binds to nuclear factor-?B p50 and represses gene expression. J Biol Chem. 2012;287:31207-17 pubmed publisher
    ..Here, we report that a euchromatic H3K9 methyltransferase, EHMT1, functions as a negative regulator in both the NF-?B- and type I interferon-mediated gene induction pathways...
  37. Chaturvedi C, Somasundaram B, Singh K, Carpenedo R, Stanford W, Dilworth F, et al. Maintenance of gene silencing by the coordinate action of the H3K9 methyltransferase G9a/KMT1C and the H3K4 demethylase Jarid1a/KDM5A. Proc Natl Acad Sci U S A. 2012;109:18845-50 pubmed publisher
  38. Balemans M, Huibers M, Eikelenboom N, Kuipers A, van Summeren R, Pijpers M, et al. Reduced exploration, increased anxiety, and altered social behavior: Autistic-like features of euchromatin histone methyltransferase 1 heterozygous knockout mice. Behav Brain Res. 2010;208:47-55 pubmed publisher
    ..defined mental retardation syndrome, caused by haplo-insufficiency of the euchromatin histone methyltransferase 1 (EHMT1) gene...
  39. Ueda J, Tachibana M, Ikura T, Shinkai Y. Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP. J Biol Chem. 2006;281:20120-8 pubmed
    ..we reported that G9a forms a stoichiometric heteromeric complex with another SET-domain-containing molecule, GLP/Eu-HMTase1...
  40. Benlhabib H, Mendelson C. Epigenetic regulation of surfactant protein A gene (SP-A) expression in fetal lung reveals a critical role for Suv39h methyltransferases during development and hypoxia. Mol Cell Biol. 2011;31:1949-58 pubmed publisher