Gene Symbol: Eed
Description: embryonic ectoderm development
Alias: l(7)5Rn, l7Rn5, lusk, polycomb protein EED, lethal, Chr 7, Rinchik 5
Species: mouse
Products:     Eed

Top Publications

  1. Kalantry S, Magnuson T. The Polycomb group protein EED is dispensable for the initiation of random X-chromosome inactivation. PLoS Genet. 2006;2:e66 pubmed
    ..Mouse embryos lacking the essential PRC2 component EED harbor defects in the maintenance of imprinted XCI in differentiating trophoblast cells...
  2. Peng J, Valouev A, Swigut T, Zhang J, Zhao Y, Sidow A, et al. Jarid2/Jumonji coordinates control of PRC2 enzymatic activity and target gene occupancy in pluripotent cells. Cell. 2009;139:1290-302 pubmed publisher
    ..Our findings illuminate a mechanism of histone methylation regulation in pluripotent cells and during early cell-fate transitions. ..
  3. García Tuñón I, Guallar D, Alonso Martin S, Benito A, Benítez Lázaro A, Pérez Palacios R, et al. Association of Rex-1 to target genes supports its interaction with Polycomb function. Stem Cell Res. 2011;7:1-16 pubmed publisher
    ..Rex-1 might fine-tune pluripotency in ES cells by modulating Polycomb-mediated gene regulation. ..
  4. Shumacher A, Faust C, Magnuson T. Positional cloning of a global regulator of anterior-posterior patterning in mice. Nature. 1996;383:250-3 pubmed
    ..Here we report the positional cloning of a classical mouse mutation, eed (for embryonic ectoderm development), which is the highly conserved homologue of the Drosophila Pc-G gene esc (for ..
  5. Morin Kensicki E, Faust C, LaMantia C, Magnuson T. Cell and tissue requirements for the gene eed during mouse gastrulation and organogenesis. Genesis. 2001;31:142-6 pubmed
    Mouse embryos homozygous for the allele eed(l7Rn5-3354SB) of the Polycomb Group gene embryonic ectoderm development (eed) display a gastrulation defect in which epiblast cells move through the streak and form extraembryonic mesoderm ..
  6. Pasini D, Bracken A, Jensen M, Lazzerini Denchi E, Helin K. Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity. EMBO J. 2004;23:4061-71 pubmed
    SUZ12 is a recently identified Polycomb group (PcG) protein, which together with EZH2 and EED forms different Polycomb repressive complexes (PRC2/3)...
  7. Shen X, Kim W, Fujiwara Y, Simon M, Liu Y, Mysliwiec M, et al. Jumonji modulates polycomb activity and self-renewal versus differentiation of stem cells. Cell. 2009;139:1303-14 pubmed publisher
    ..Our results demonstrate that dynamic regulation of Polycomb complex activity orchestrated by JMJ balances self-renewal and differentiation, highlighting the involvement of chromatin dynamics in cell-fate transitions. ..
  8. Okamoto I, Otte A, Allis C, Reinberg D, Heard E. Epigenetic dynamics of imprinted X inactivation during early mouse development. Science. 2004;303:644-9 pubmed
    ..This reveals the remarkable plasticity of the X-inactivation process during preimplantation development and underlines the importance of the ICM in global reprogramming of epigenetic marks in the early embryo...
  9. Plath K, Talbot D, Hamer K, Otte A, Yang T, Jaenisch R, et al. Developmentally regulated alterations in Polycomb repressive complex 1 proteins on the inactive X chromosome. J Cell Biol. 2004;167:1025-35 pubmed
    ..Our results implicate mPRC1 in X inactivation and suggest that the regulated assembly of PcG protein complexes on the Xi contributes to this multistep process. ..

More Information


  1. Mak W, Baxter J, Silva J, Newall A, Otte A, Brockdorff N. Mitotically stable association of polycomb group proteins eed and enx1 with the inactive x chromosome in trophoblast stem cells. Curr Biol. 2002;12:1016-20 pubmed has shown that imprinted X inactivation in trophectoderm is not maintained in embryonic ectoderm development (eed) mutant mice...
  2. Erhardt S, Su I, Schneider R, Barton S, Bannister A, Perez Burgos L, et al. Consequences of the depletion of zygotic and embryonic enhancer of zeste 2 during preimplantation mouse development. Development. 2003;130:4235-48 pubmed
    ..This phenotypic effect on growth could be attributed to the asymmetric localisation of the Ezh2/Eed complex and the associated histone methylation pattern to the maternal genome, which is disrupted in Ezh2 mutant ..
  3. Montgomery N, Yee D, Chen A, Kalantry S, Chamberlain S, Otte A, et al. The murine polycomb group protein Eed is required for global histone H3 lysine-27 methylation. Curr Biol. 2005;15:942-7 pubmed
    ..PRC2, is composed of several proteins including the histone methyltransferase (HMTase) Ezh2, the WD-repeat protein Eed, and the Zn-finger protein Suz12...
  4. Kalantry S, Mills K, Yee D, Otte A, Panning B, Magnuson T. The Polycomb group protein Eed protects the inactive X-chromosome from differentiation-induced reactivation. Nat Cell Biol. 2006;8:195-202 pubmed
    ..In mouse embryos that are mutated for the PcG protein Eed, X-chromosome inactivation (XCI) is not stably maintained in extra-embryonic tissues...
  5. Boyer L, Plath K, Zeitlinger J, Brambrink T, Medeiros L, Lee T, et al. Polycomb complexes repress developmental regulators in murine embryonic stem cells. Nature. 2006;441:349-53 pubmed
    ..role in gene silencing in ES cells, PcG target genes were de-repressed in cells deficient for the PRC2 component Eed, and were preferentially activated on induction of differentiation...
  6. Wang J, Mager J, Chen Y, Schneider E, Cross J, Nagy A, et al. Imprinted X inactivation maintained by a mouse Polycomb group gene. Nat Genet. 2001;28:371-5 pubmed
    ..Here we show that the gene eed (embryonic ectoderm development), a member of the mouse Polycomb group (Pc-G) of genes, is required for primary and ..
  7. Kim S, Paylor S, Magnuson T, Schumacher A. Juxtaposed Polycomb complexes co-regulate vertebral identity. Development. 2006;133:4957-68 pubmed
    ..The present study interrogated the genetic, biochemical and molecular interaction of BMI1 and EED, pivotal constituents of heterologous Polycomb complexes, in the regulation of vertebral identity during mouse ..
  8. Puschendorf M, Terranova R, Boutsma E, Mao X, Isono K, Brykczynska U, et al. PRC1 and Suv39h specify parental asymmetry at constitutive heterochromatin in early mouse embryos. Nat Genet. 2008;40:411-20 pubmed publisher
    ..Parental epigenetic asymmetry, also observed along cleavage chromosomes, is resolved by the end of the 8-cell stage--concurrent with blastomere polarization--marking the end of the maternal-to-embryonic transition. ..
  9. Wang J, Mager J, Schnedier E, Magnuson T. The mouse PcG gene eed is required for Hox gene repression and extraembryonic development. Mamm Genome. 2002;13:493-503 pubmed
    ..In mice, the PcG protein Eed (Embryonic ectoderm development) is present in a distinct complex that interacts with histone deacetylase (HDAC) ..
  10. Walker E, Chang W, Hunkapiller J, Cagney G, Garcha K, Torchia J, et al. Polycomb-like 2 associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell self-renewal and differentiation. Cell Stem Cell. 2010;6:153-66 pubmed publisher
    ..We describe the role of PCL2 in both modulating transcription of ESC self-renewal genes in undifferentiated ESCs as well as developmental regulators during early commitment and differentiation. ..
  11. Faust C, Schumacher A, Holdener B, Magnuson T. The eed mutation disrupts anterior mesoderm production in mice. Development. 1995;121:273-85 pubmed
    Mouse embryos homozygous for the mutation embryonic ectoderm development (eed) exhibit a growth defect and fail to gastrulate normally...
  12. Rinchik E, Carpenter D. N-ethyl-N-nitrosourea-induced prenatally lethal mutations define at least two complementation groups within the embryonic ectoderm development (eed) locus in mouse chromosome 7. Mamm Genome. 1993;4:349-53 pubmed
    ..Both loci were found to map to the subregion of the Mod-2-sh-1 interval that contains the eed (embryonic ectoderm development) locus, eed has been defined by the inability of embryos homozygous for certain c ..
  13. Mager J, Montgomery N, de Villena F, Magnuson T. Genome imprinting regulated by the mouse Polycomb group protein Eed. Nat Genet. 2003;33:502-7 pubmed
    ..It has recently been found that the mouse Polycomb group (PcG) gene Eed (embryonic ectoderm development) acts to maintain repression of the imprinted X chromosome...
  14. Faust C, Lawson K, Schork N, Thiel B, Magnuson T. The Polycomb-group gene eed is required for normal morphogenetic movements during gastrulation in the mouse embryo. Development. 1998;125:4495-506 pubmed
    We have characterized an induced mutation, called embryonic ectoderm development or eed, that disrupts A-P patterning of the mouse embryo during gastrulation...
  15. Voncken J, Roelen B, Roefs M, de Vries S, Verhoeven E, Marino S, et al. Rnf2 (Ring1b) deficiency causes gastrulation arrest and cell cycle inhibition. Proc Natl Acad Sci U S A. 2003;100:2468-73 pubmed
    ..The early lethal phenotype is reminiscent of that of the PcG-gene knockouts Eed and Ezh2, which belong to a separate functional PcG group and PcG protein complex...
  16. Plath K, Fang J, Mlynarczyk Evans S, Cao R, Worringer K, Wang H, et al. Role of histone H3 lysine 27 methylation in X inactivation. Science. 2003;300:131-5 pubmed
    The Polycomb group (PcG) protein Eed is implicated in regulation of imprinted X-chromosome inactivation in extraembryonic cells but not of random X inactivation in embryonic cells...
  17. Pasini D, Cloos P, Walfridsson J, Olsson L, Bukowski J, Johansen J, et al. JARID2 regulates binding of the Polycomb repressive complex 2 to target genes in ES cells. Nature. 2010;464:306-10 pubmed publisher
    ..Thus, these results demonstrate that JARID2 is essential for the binding of PcG proteins to target genes and, consistent with this, for the proper differentiation of embryonic stem cells and normal development. ..
  18. de Napoles M, Mermoud J, Wakao R, Tang Y, Endoh M, Appanah R, et al. Polycomb group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation. Dev Cell. 2004;7:663-76 pubmed
    ..These observations link H2A ubiquitylation, X inactivation, and PRC1 PcG function, suggesting an unanticipated and novel mechanism for chromatin-mediated heritable gene silencing. ..
  19. Saha B, Home P, Ray S, Larson M, Paul A, Rajendran G, et al. EED and KDM6B coordinate the first mammalian cell lineage commitment to ensure embryo implantation. Mol Cell Biol. 2013;33:2691-705 pubmed publisher
    ..of the TE and ICM lineages is coordinated via combinatorial regulation of embryonic ectoderm development (EED) and lysine-specific demethylase 6B (KDM6B)...
  20. Ura H, Murakami K, Akagi T, Kinoshita K, Yamaguchi S, Masui S, et al. Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation. EMBO J. 2011;30:2190-204 pubmed publisher
    ..ES cells between Sox2, an indispensable transcription factor for self-renewal, and embryonic ectoderm development (Eed), an epigenetic modulator regulating histone methylation...
  21. Montgomery N, Yee D, Montgomery S, Magnuson T. Molecular and functional mapping of EED motifs required for PRC2-dependent histone methylation. J Mol Biol. 2007;374:1145-57 pubmed
    ..27 (H3K27) methyltransferase enhancer of zeste homolog 2 and the WD-repeat protein embryonic ectoderm development (EED). Histone H3K27 can be monomethylated (H3K27me1), dimethylated (H3K27me2), or trimethylated (H3K27me3)...
  22. Sharan S, Holdener Kenny B, Threadgill D, Magnuson T. Genomic mapping within the albino-deletion complex using individual early postimplantation mouse embryos. Mamm Genome. 1992;3:79-83 pubmed
    ..Using this procedure, we have been able to redefine the distal limits of the region of Chromosome (Chr) 7 containing a gene (eed) needed for development of the embryonic ectoderm.
  23. Zhang Y, Teng F, Luo G, Wang M, Tong M, Zhao X, et al. MicroRNA-323-3p regulates the activity of polycomb repressive complex 2 (PRC2) via targeting the mRNA of embryonic ectoderm development (Eed) gene in mouse embryonic stem cells. J Biol Chem. 2013;288:23659-65 pubmed publisher
    ..Here, we show that a microRNA encoded by the imprinted Dlk1-Dio3 region of mouse chromosome 12, miR-323-3p, targets Eed (embryonic ectoderm development) mRNA, which encodes one of the core components of PRC2, the EED protein...
  24. Zheng H, Tsukahara M, Liu E, Ye L, Xiong H, Noguchi S, et al. The novel helicase helG (DHX30) is expressed during gastrulation in mice and has a structure similar to a human DExH box helicase. Stem Cells Dev. 2015;24:372-83 pubmed publisher
  25. Fernandez Valdivia R, Zhang Y, Pai S, Metzker M, Schumacher A. l7Rn6 encodes a novel protein required for clara cell function in mouse lung development. Genetics. 2006;172:389-99 pubmed
    ..Thus, l7Rn6 represents a novel protein required for organization and/or function of the secretory apparatus in Clara cells in mouse lung. ..
  26. Denisenko O, Bomsztyk K. The product of the murine homolog of the Drosophila extra sex combs gene displays transcriptional repressor activity. Mol Cell Biol. 1997;17:4707-17 pubmed
    ..we isolated is identical to the cDNA of the recently positionally cloned mouse embryonic ectoderm development gene, eed. Like Esc, Eed contains six WD-40 repeats in the C-terminal half of the protein and is thought to repress homeotic ..
  27. Majewski I, Ritchie M, Phipson B, Corbin J, Pakusch M, Ebert A, et al. Opposing roles of polycomb repressive complexes in hematopoietic stem and progenitor cells. Blood. 2010;116:731-9 pubmed publisher
    ..These studies provide new insights into the mechanistic interplay between distinct PRCs and have important implications for approaching PcG proteins as therapeutic targets. ..
  28. Miro X, Zhou X, Boretius S, Michaelis T, Kubisch C, Alvarez Bolado G, et al. Haploinsufficiency of the murine polycomb gene Suz12 results in diverse malformations of the brain and neural tube. Dis Model Mech. 2009;2:412-8 pubmed publisher
    ..Our work strongly suggests that brain malformations such as CM can result from altered epigenetic regulation of genes involved in cell proliferation in the brain. ..
  29. Fujimura Y, Isono K, Vidal M, Endoh M, Kajita H, Mizutani Koseki Y, et al. Distinct roles of Polycomb group gene products in transcriptionally repressed and active domains of Hoxb8. Development. 2006;133:2371-81 pubmed
    ..The present study indicates distinct roles for class 2 PcG complexes in transcriptionally repressed and active domains of Hoxb8 gene. ..
  30. Ballaré C, Lange M, Lapinaite A, Martin G, Morey L, Pascual G, et al. Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity. Nat Struct Mol Biol. 2012;19:1257-65 pubmed publisher
    ..These findings show that the interaction of Phf19 with H3K36me2 and H3K36me3 is essential for PRC2 complex activity and for proper regulation of gene repression in embryonic stem cells. ..
  31. Whitcomb S, Fierz B, McGinty R, Holt M, Ito T, Muir T, et al. Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2. J Biol Chem. 2012;287:23718-25 pubmed publisher
    ..Taken together, these results highlight how the position of nucleosome monoubiquitylation affects the specificity and direction of cross-talk with enzymatic activities on chromatin. ..
  32. Lai H, Wang Q. Additional sex combs-like 2 is required for polycomb repressive complex 2 binding at select targets. PLoS ONE. 2013;8:e73983 pubmed publisher
    ..ASXL2 is required for the binding of PRC2 and for the enrichment of H3K27me3 at target promoters. These results add a new perspective to our understanding of the mechanisms that regulate PcG activity and gene repression. ..
  33. Holdener B, Thomas J, Schumacher A, Potter M, Rinchik E, Sharan S, et al. Physical localization of eed: a region of mouse chromosome 7 required for gastrulation. Genomics. 1995;27:447-56 pubmed
    ..Two loci, identified by chemical mutagenesis, are known to map within the eed interval. One, l7Rn5, probably represents the gene required for gastrulation. The second, l7Rn6, is required for survival after birth...
  34. Schumacher A, Faust C, Magnuson T. Positional cloning of a global regulator of anterior-posterior patterning in mice. Nature. 1996;384:648 pubmed
  35. Monk D, Wagschal A, Arnaud P, Müller P, Parker Katiraee L, Bourc his D, et al. Comparative analysis of human chromosome 7q21 and mouse proximal chromosome 6 reveals a placental-specific imprinted gene, TFPI2/Tfpi2, which requires EHMT2 and EED for allelic-silencing. Genome Res. 2008;18:1270-81 pubmed publisher
    ..Deficiency for the histone methyltransferase EHMT2 (also known as G9A) or for the Polycomb group protein EED, involved in repressive H3K9me2 and H3K27me3 respectively, leads to biallelic expression of Tfpi2 in the extra-..
  36. Hisada K, Sanchez C, Endo T, Endoh M, Román Trufero M, Sharif J, et al. RYBP represses endogenous retroviruses and preimplantation- and germ line-specific genes in mouse embryonic stem cells. Mol Cell Biol. 2012;32:1139-49 pubmed publisher
    ..We discovered, using Eed-knockout (KO) ES cells, that RYBP binding to promoters was independent of H3K27me3...
  37. Ma K, Hung H, Srinivasan R, Xie H, Orkin S, Svaren J. Regulation of Peripheral Nerve Myelin Maintenance by Gene Repression through Polycomb Repressive Complex 2. J Neurosci. 2015;35:8640-52 pubmed publisher
    ..the role of histone H3 Lys27 methylation, we have generated mice with a Schwann cell-specific knock-out of Eed, which is an essential subunit of the polycomb repressive complex 2 (PRC2) that catalyzes methylation of histone H3 ..
  38. Obier N, Lin Q, Cauchy P, Hornich V, Zenke M, Becker M, et al. Polycomb protein EED is required for silencing of pluripotency genes upon ESC differentiation. Stem Cell Rev. 2015;11:50-61 pubmed publisher
    b>Eed (embryonic ectoderm development) is a core component of the Polycomb Repressive Complex 2 (PRC2) which catalyzes the methylation of histone H3 lysine 27 (H3K27)...
  39. Dauber K, Perdigoto C, Valdes V, Santoriello F, Cohen I, Ezhkova E. Dissecting the Roles of Polycomb Repressive Complex 2 Subunits in the Control of Skin Development. J Invest Dermatol. 2016;136:1647-1655 pubmed publisher
    ..three conditional knockout mouse lines, in which the essential PRC2 subunits embryonic ectoderm development (EED), suppressor of zeste 12 homolog (Suz12), and enhancer of zeste homologs 1 and 2 (Ezh1/2) are conditionally ablated ..
  40. Hagarman J, Motley M, Kristjansdottir K, Soloway P. Coordinate regulation of DNA methylation and H3K27me3 in mouse embryonic stem cells. PLoS ONE. 2013;8:e53880 pubmed publisher
  41. Denisenko O, Shnyreva M, Suzuki H, Bomsztyk K. Point mutations in the WD40 domain of Eed block its interaction with Ezh2. Mol Cell Biol. 1998;18:5634-42 pubmed
    ..To explore the nature of these complexes and their function, we used the full-length Eed (embryonic ectoderm development) protein, a mammalian homolog of the Drosophila Polycomb group protein Esc, as a ..
  42. Danis E, Yamauchi T, Echanique K, Zhang X, Haladyna J, Riedel S, et al. Ezh2 Controls an Early Hematopoietic Program and Growth and Survival Signaling in Early T Cell Precursor Acute Lymphoblastic Leukemia. Cell Rep. 2016;14:1953-65 pubmed publisher
    ..Our data mechanistically link Ezh2 inactivation to stem-cell-associated transcriptional programs and increased growth/survival signaling, features that convey an adverse prognosis in patients. ..
  43. Fedoriw A, Calabrese J, Mu W, Yee D, Magnuson T. Differentiation-driven nucleolar association of the mouse imprinted Kcnq1 locus. G3 (Bethesda). 2012;2:1521-8 pubmed publisher
    ..Although nucleolar positioning has been proposed as a mechanism to related to gene silencing, we find that silencing and perinucleolar localization through the Kcnq1ot1 noncoding RNA are separable events. ..
  44. Sun B, Chang E, Gerhartl A, Szele F. Polycomb Protein Eed is Required for Neurogenesis and Cortical Injury Activation in the Subventricular Zone. Cereb Cortex. 2018;28:1369-1382 pubmed publisher
    ..We report that label-retaining SVZ NSCs express Eed, the core component of Polycomb repressive complex 2...
  45. Rinchik E, Carpenter D. N-ethyl-N-nitrosourea mutagenesis of a 6- to 11-cM subregion of the Fah-Hbb interval of mouse chromosome 7: Completed testing of 4557 gametes and deletion mapping and complementation analysis of 31 mutations. Genetics. 1999;152:373-83 pubmed
    ..g., locus mutability, failure to saturate, number of gametes to screen, allelic series) of concern when application of chemical mutagenesis screens to megabase regions of the mouse genome is considered. ..
  46. Roy N, Van Eynde A, Beke L, Nuytten M, Bollen M. The transcriptional repression by NIPP1 is mediated by Polycomb group proteins. Biochim Biophys Acta. 2007;1769:541-5 pubmed
    ..Here we show that the transcriptional repression by NIPP1 is alleviated by the RNAi-mediated knockdown of EED and EZH2, two core components of the Polycomb Repressive Complex 2 (PRC2), and by the overexpression of a ..
  47. Pereira C, Piccolo F, Tsubouchi T, Sauer S, Ryan N, Bruno L, et al. ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency. Cell Stem Cell. 2010;6:547-56 pubmed publisher
  48. Yu M, Riva L, Xie H, Schindler Y, Moran T, Cheng Y, et al. Insights into GATA-1-mediated gene activation versus repression via genome-wide chromatin occupancy analysis. Mol Cell. 2009;36:682-95 pubmed publisher
    ..These data provide insights into GATA-1-mediated gene regulation in vivo. ..
  49. Zakin L, Reversade B, Virlon B, Rusniok C, Glaser P, Elalouf J, et al. Gene expression profiles in normal and Otx2-/- early gastrulating mouse embryos. Proc Natl Acad Sci U S A. 2000;97:14388-93 pubmed
    ..Among a broader list, the study of six genes found to be differentially expressed allows defining a role for Otx2 in the orchestration of cell movements leading to the adequate organization of the embryo before gastrulation. ..
  50. Kim S, Levenson J, Korsmeyer S, Sweatt J, Schumacher A. Developmental regulation of Eed complex composition governs a switch in global histone modification in brain. J Biol Chem. 2007;282:9962-72 pubmed
    ..This study identified a novel, developmentally regulated interface between Eed and Mll, pivotal constituents of PcG and trxG pathways, respectively, in mouse brain...
  51. Shibata S, Yokota T, Wutz A. Synergy of Eed and Tsix in the repression of Xist gene and X-chromosome inactivation. EMBO J. 2008;27:1816-26 pubmed publisher
    ..Here, we provide evidence that the histone methyltransferase Eed is also involved in the process...
  52. Serresi M, Gargiulo G, Proost N, Siteur B, Cesaroni M, Koppens M, et al. Polycomb Repressive Complex 2 Is a Barrier to KRAS-Driven Inflammation and Epithelial-Mesenchymal Transition in Non-Small-Cell Lung Cancer. Cancer Cell. 2016;29:17-31 pubmed publisher
    ..Modulation of PRC2 by either Ezh2 overexpression or Eed deletion enhances KRAS-driven adenomagenesis and inflammation, respectively...
  53. Lessard J, Schumacher A, Thorsteinsdottir U, van Lohuizen M, Magnuson T, Sauvageau G. Functional antagonism of the Polycomb-Group genes eed and Bmi1 in hemopoietic cell proliferation. Genes Dev. 1999;13:2691-703 pubmed
    The murine Polycomb-Group (PcG) proteins Eed and Bmi1 govern axial patterning during embryonic development by segment-specific repression of Hox gene expression...
  54. Ura H, Usuda M, Kinoshita K, Sun C, Mori K, Akagi T, et al. STAT3 and Oct-3/4 control histone modification through induction of Eed in embryonic stem cells. J Biol Chem. 2008;283:9713-23 pubmed publisher
    ..In the present study, we identified Eed, a core component of Polycomb repressive complex 2, as a downstream molecule of STAT3 and Oct-3/4...
  55. van Lohuizen M, Tijms M, Voncken J, Schumacher A, Magnuson T, Wientjens E. Interaction of mouse polycomb-group (Pc-G) proteins Enx1 and Enx2 with Eed: indication for separate Pc-G complexes. Mol Cell Biol. 1998;18:3572-9 pubmed
    ..Drosophila extra sex combs (esc) and its mammalian homolog embryonic ectoderm development (eed) are special Pc-G members, in that they are required early during development when Pc-G repression is initiated, a ..
  56. Ai S, Peng Y, Li C, Gu F, Yu X, Yue Y, et al. EED orchestration of heart maturation through interaction with HDACs is H3K27me3-independent. elife. 2017;6: pubmed publisher
    ..b>EED (embryonic ectoderm development) inactivation in the postnatal heart (EedCKO) caused lethal dilated ..
  57. O Hagan H, Wang W, Sen S, DeStefano Shields C, Lee S, Zhang Y, et al. Oxidative damage targets complexes containing DNA methyltransferases, SIRT1, and polycomb members to promoter CpG Islands. Cancer Cell. 2011;20:606-19 pubmed publisher
    ..Thus, oxidative damage induces formation and relocalization of a silencing complex that may explain cancer-specific aberrant DNA methylation and transcriptional silencing. ..
  58. Dietrich N, Lerdrup M, Landt E, Agrawal Singh S, Bak M, Tommerup N, et al. REST-mediated recruitment of polycomb repressor complexes in mammalian cells. PLoS Genet. 2012;8:e1002494 pubmed publisher
    ..Genome-wide analysis of Polycomb binding in Rest⁻/⁻ and Eed⁻/⁻ mouse embryonic stem (mES) cells showed that Rest was required for PRC1 recruitment to a subset of ..
  59. Showell C, Cunliffe V. Identification of putative interaction partners for the Xenopus Polycomb-group protein Xeed. Gene. 2002;291:95-104 pubmed
    The extra sex combs (esc) gene of Drosophila and its mammalian homologue embryonic ectoderm development (eed) play pivotal roles in establishing Polycomb-group (Pc-G) mediated transcriptional silencing of regulatory genes during early ..
  60. Mirzamohammadi F, Papaioannou G, Inloes J, Rankin E, Xie H, Schipani E, et al. Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-? signalling. Nat Commun. 2016;7:12047 pubmed publisher
    ..However, the role of PRC2 in lineage-committed somatic cells is mostly unknown. Here we show that Eed deficiency in chondrocytes causes severe kyphosis and a growth defect with decreased chondrocyte proliferation, ..
  61. He A, Ma Q, Cao J, von Gise A, Zhou P, Xie H, et al. Polycomb repressive complex 2 regulates normal development of the mouse heart. Circ Res. 2012;110:406-15 pubmed publisher
    ..later in heart development, as indicated by cardiomyocyte-restricted TNT-Cre inactivation of the PRC2 subunit Eed. However, Ezh2 inactivation by TNT-Cre did not cause an overt phenotype, likely because of functional redundancy ..
  62. Danis E, Yamauchi T, Echanique K, Haladyna J, Kalkur R, Riedel S, et al. Inactivation of Eed impedes MLL-AF9-mediated leukemogenesis through Cdkn2a-dependent and Cdkn2a-independent mechanisms in a murine model. Exp Hematol. 2015;43:930-935.e6 pubmed publisher
    ..ablation of canonical PRC2 function by genetic inactivation of the core component embryonic ectoderm development (Eed) or by combined pharmacologic inhibition of the PRC2 methyltransferases EZH2 and EZH1 has a strong anti-leukemic ..
  63. Shaw T, Martin P. Epigenetic reprogramming during wound healing: loss of polycomb-mediated silencing may enable upregulation of repair genes. EMBO Rep. 2009;10:881-6 pubmed publisher
    ..We show that PcGs Eed, Ezh2 and Suz12 are significantly downregulated during murine skin repair, whereas the newly described demethylases ..
  64. Nichols J, Silva J, Roode M, Smith A. Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo. Development. 2009;136:3215-22 pubmed publisher
    ..We propose that ES cells are not a tissue culture creation, but are essentially identical to pre-implantation epiblast cells. ..
  65. Maclary E, Hinten M, Harris C, Sethuraman S, Gayen S, Kalantry S. PRC2 represses transcribed genes on the imprinted inactive X chromosome in mice. Genome Biol. 2017;18:82 pubmed publisher
    ..To abrogate PRC2 function, we delete the core PRC2 protein EED in F1 hybrid trophoblast stem cells (TSCs), which undergo imprinted inactivation of the paternally inherited X ..
  66. Ferguson Smith A, Reik W. The need for Eed. Nat Genet. 2003;33:433-4 pubmed
  67. Richie E, Schumacher A, Angel J, Holloway M, Rinchik E, Magnuson T. The Polycomb-group gene eed regulates thymocyte differentiation and suppresses the development of carcinogen-induced T-cell lymphomas. Oncogene. 2002;21:299-306 pubmed
    The mouse Polycomb-group gene, embryonic ectoderm development (eed), appears to regulate cellular growth and differentiation in a developmental and tissue specific manner...
  68. Alexander M, Mlynarczyk Evans S, Royce Tolland M, Plocik A, Kalantry S, Magnuson T, et al. Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed. J Cell Biol. 2007;179:269-76 pubmed
    ..The Polycomb Group gene Eed is required to establish this distinctive behavior...
  69. Wines M, Tiffany A, Holdener B. Physical localization of the mouse aryl hydrocarbon receptor nuclear translocator-2 (Arnt2) gene within the c112K deletion. Genomics. 1998;51:223-32 pubmed
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    ..Our data indicate that bivalent chromatin, in combination with neuronal factors, controls the paternal expression of Grb10 in brain. This finding highlights a novel mechanism to control tissue-specific imprinting. ..
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    ..These defects are associated with impaired SC proliferation and derepression of genes expressed in nonmuscle cell lineages. Thus, EZH2 controls self-renewal and proliferation, and maintains an appropriate transcriptional program in SCs. ..