Drosha

Summary

Gene Symbol: Drosha
Description: drosha, ribonuclease type III
Alias: 1110013A17Rik, AI874853, Etohi2, Rn3, Rnasen, ribonuclease 3, RNase III, ethanol induced 2, protein Drosha, ribonuclease III, nuclear
Species: mouse
Products:     Drosha

Top Publications

  1. Chong M, Zhang G, Cheloufi S, Neubert T, Hannon G, Littman D. Canonical and alternate functions of the microRNA biogenesis machinery. Genes Dev. 2010;24:1951-60 pubmed publisher
    The canonical microRNA (miRNA) biogenesis pathway requires two RNaseIII enzymes: Drosha and Dicer...
  2. Cobb B, Hertweck A, Smith J, O Connor E, Graf D, Cook T, et al. A role for Dicer in immune regulation. J Exp Med. 2006;203:2519-27 pubmed
    ..Depleting miRNAs by eliminating Dicer, the RNAse III enzyme that generates functional miRNAs, reduces T reg cell numbers and results in immune pathology...
  3. Davis T, Cuellar T, Koch S, Barker A, Harfe B, McManus M, et al. Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus. J Neurosci. 2008;28:4322-30 pubmed publisher
    To investigate the role of Dicer and microRNAs in the mammalian CNS, we used mice in which the second RNase III domain of Dicer was conditionally floxed...
  4. Cheloufi S, Dos Santos C, Chong M, Hannon G. A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature. 2010;465:584-9 pubmed publisher
    ..Though this microRNA is processed by Drosha (also known as Rnasen), its maturation does not require Dicer...
  5. Chong M, Rasmussen J, Rudensky A, Rundensky A, Littman D. The RNAseIII enzyme Drosha is critical in T cells for preventing lethal inflammatory disease. J Exp Med. 2008;205:2005-17 pubmed publisher
    ..We provide genetic and in vivo evidence that the two RNaseIII enzymes, Drosha and Dicer, do indeed function in the same pathway...
  6. Gruber J, Zatechka D, Sabin L, Yong J, Lum J, Kong M, et al. Ars2 links the nuclear cap-binding complex to RNA interference and cell proliferation. Cell. 2009;138:328-39 pubmed publisher
    ..These findings provide evidence for a role for Ars2 in RNA interference regulation during cell proliferation. ..
  7. Shenoy A, Blelloch R. Genomic analysis suggests that mRNA destabilization by the microprocessor is specialized for the auto-regulation of Dgcr8. PLoS ONE. 2009;4:e6971 pubmed publisher
    The Microprocessor, containing the RNA binding protein Dgcr8 and RNase III enzyme Drosha, is responsible for processing primary microRNAs to precursor microRNAs...
  8. Song R, Hennig G, Wu Q, Jose C, Zheng H, Yan W. Male germ cells express abundant endogenous siRNAs. Proc Natl Acad Sci U S A. 2011;108:13159-64 pubmed publisher
    ..The biogenesis of these testicular endo-siRNAs is DROSHA independent, but DICER dependent...
  9. Kirigin F, Lindstedt K, Sellars M, Ciofani M, Low S, Jones L, et al. Dynamic microRNA gene transcription and processing during T cell development. J Immunol. 2012;188:3257-67 pubmed publisher
    ..The longest was pri-mir-29b-1/29a at ?168 kb. All the long pri-miRNAs also displayed extensive splicing. Our findings indicate that miRNA expression during T cell development is both a highly dynamic and a highly regulated process. ..

More Information

Publications48

  1. Knuckles P, Vogt M, Lugert S, Milo M, Chong M, Hautbergue G, et al. Drosha regulates neurogenesis by controlling neurogenin 2 expression independent of microRNAs. Nat Neurosci. 2012;15:962-9 pubmed publisher
    ..The mechanism of the process is unclear. Here we show that the RNase III Drosha and DGCR8 (also known as Pasha), key components of the microRNA (miRNA) microprocessor, have important ..
  2. Shapiro J, Schmid S, Aguado L, Sabin L, Yasunaga A, Shim J, et al. Drosha as an interferon-independent antiviral factor. Proc Natl Acad Sci U S A. 2014;111:7108-13 pubmed publisher
    ..virus infection in mammals, we evaluated antiviral activity in the presence and absence of Dicer or Drosha, the RNase III nucleases responsible for generating small RNAs...
  3. Motiño O, Francés D, Mayoral R, Castro Sánchez L, Fernández Velasco M, Boscá L, et al. Regulation of MicroRNA 183 by Cyclooxygenase 2 in Liver Is DEAD-Box Helicase p68 (DDX5) Dependent: Role in Insulin Signaling. Mol Cell Biol. 2015;35:2554-67 pubmed publisher
    ..DDX5) through phosphatidylinositol 3-kinase (PI3K)/p300 signaling and by modulating the enzymatic function of the Drosha (RNase type III) complex through its physical association with DDX5...
  4. Lee D, Nam J, Shin C. DROSHA targets its own transcript to modulate alternative splicing. RNA. 2017;23:1035-1047 pubmed publisher
    The nuclear RNase III enzyme DROSHA interacts with its cofactor DGCR8 to form the Microprocessor complex, which initiates microRNA (miRNA) maturation by cleaving hairpin structures embedded in primary transcripts...
  5. Jiang X, Wooderchak Donahue W, McDonald J, Ghatpande P, Baalbaki M, Sandoval M, et al. Inactivating mutations in Drosha mediate vascular abnormalities similar to hereditary hemorrhagic telangiectasia. Sci Signal. 2018;11: pubmed publisher
    ..b>Drosha, a key enzyme for microRNA (miRNA) biogenesis, also regulates the TGF-β and BMP pathway through interaction ..
  6. Kobayashi T, Papaioannou G, Mirzamohammadi F, Kozhemyakina E, Zhang M, Blelloch R, et al. Early postnatal ablation of the microRNA-processing enzyme, Drosha, causes chondrocyte death and impairs the structural integrity of the articular cartilage. Osteoarthritis Cartilage. 2015;23:1214-20 pubmed publisher
    ..of generating a novel mouse osteoarthritis (OA) model caused by intrinsic cellular dysfunction, we ablated Drosha, another essential enzyme for miRNA biogenesis, exclusively in articular chondrocytes of postnatal mice...
  7. Jiang X, Hawkins J, Lee J, Lizama C, Bos F, Zape J, et al. Let-7 microRNA-dependent control of leukotriene signaling regulates the transition of hematopoietic niche in mice. Nat Commun. 2017;8:128 pubmed publisher
    ..Here we report that vascular endothelium-specific deletion of mouse Drosha (Drosha cKO), an enzyme essential for microRNA biogenesis, leads to anemia and death...
  8. Stathopoulou A, Chhetri J, Ambrose J, Esteve P, Ji L, Erdjument Bromage H, et al. A novel requirement for DROSHA in maintenance of mammalian CG methylation. Nucleic Acids Res. 2017;45:9398-9412 pubmed publisher
    ..Using a protein interaction screen, we identify Microprocessor component DROSHA as a novel DNMT1-interactor...
  9. Rout U, Krawetz S, Armant D. Ethanol-induced intracellular calcium mobilization rapidly alters gene expression in the mouse blastocyst. Cell Calcium. 1997;22:463-74 pubmed
    ..Increased expression of arginase and other genes may mediate the onset of rapid cell proliferation and differentiation that is induced by Ca2+ signaling during pre-implantation development. ..
  10. Teta M, Choi Y, Okegbe T, Wong G, Tam O, Chong M, et al. Inducible deletion of epidermal Dicer and Drosha reveals multiple functions for miRNAs in postnatal skin. Development. 2012;139:1405-16 pubmed publisher
    ..We compared the effects of deleting the essential miRNA biogenesis enzymes Drosha and Dicer in mouse skin epithelial cells at successive postnatal time points...
  11. FORTIN K, Nicholson R, Nicholson A. Mouse ribonuclease III. cDNA structure, expression analysis, and chromosomal location. BMC Genomics. 2002;3:26 pubmed
    ..A human RNase III orthologue has been implicated in ribosomal RNA maturation...
  12. Yang W, Yang D, Na S, Sandusky G, Zhang Q, Zhao G. Dicer is required for embryonic angiogenesis during mouse development. J Biol Chem. 2005;280:9330-5 pubmed
  13. Akagi K, Li J, Stephens R, Volfovsky N, Symer D. Extensive variation between inbred mouse strains due to endogenous L1 retrotransposition. Genome Res. 2008;18:869-80 pubmed publisher
    ..structures and expression are altered directly by polymorphic L1 retrotransposons, including Drosha (also called Rnasen), Parp8, Scn1a, Arhgap15, and others, including novel genes...
  14. Phua S, Sivakamasundari V, Shao Y, Cai X, Zhang L, Lufkin T, et al. Nuclear accumulation of an uncapped RNA produced by Drosha cleavage of a transcript encoding miR-10b and HOXD4. PLoS ONE. 2011;6:e25689 pubmed publisher
    ..transcripts are uncapped, together with the mapping of their 5' ends, strongly suggests that they are generated by Drosha cleavage of P2 transcripts rather than by transcriptional initiation...
  15. Michon F, Tummers M, Kyyrönen M, Frilander M, Thesleff I. Tooth morphogenesis and ameloblast differentiation are regulated by micro-RNAs. Dev Biol. 2010;340:355-68 pubmed publisher
    ..Based on our findings we suggest that miRNAs modulate tooth morphogenesis largely by fine tuning conserved signaling networks and that miRNAs may have played important roles during tooth evolution...
  16. Wu H, Ye C, Ramirez D, Manjunath N. Alternative processing of primary microRNA transcripts by Drosha generates 5' end variation of mature microRNA. PLoS ONE. 2009;4:e7566 pubmed publisher
    ..Here we show that a shift in the Drosha processing of pri-miRNA generates multiple forms of miR-142s in vivo with differing 5' ends that might target ..
  17. Rolando C, Erni A, Grison A, Beattie R, Engler A, Gokhale P, et al. Multipotency of Adult Hippocampal NSCs In Vivo Is Restricted by Drosha/NFIB. Cell Stem Cell. 2016;19:653-662 pubmed publisher
    ..Here, we report that deletion of Drosha in adult dentate gyrus NSCs activates oligodendrogenesis and reduces neurogenesis at the expense of gliogenesis...
  18. Rowley J, Chappaz S, Corduan A, Chong M, Campbell R, Khoury A, et al. Dicer1-mediated miRNA processing shapes the mRNA profile and function of murine platelets. Blood. 2016;127:1743-51 pubmed publisher
    ..CombinedPf4-cre-mediated deletion of Drosha and Dicer1 did not significantly exacerbate phenotypes observed in single Dicer1 knockout mice...
  19. Krol J, Krol I, Alvarez C, Fiscella M, Hierlemann A, Roska B, et al. A network comprising short and long noncoding RNAs and RNA helicase controls mouse retina architecture. Nat Commun. 2015;6:7305 pubmed publisher
    ..Our results suggest that the precise timing of glia-neuron interaction controlled by noncoding RNAs and Ddx3x is important for the even distribution of cells across layers. ..
  20. Rybak A, Fuchs H, Smirnova L, Brandt C, Pohl E, Nitsch R, et al. A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitment. Nat Cell Biol. 2008;10:987-93 pubmed publisher
  21. Yuan S, Ortogero N, Wu Q, Zheng H, Yan W. Murine follicular development requires oocyte DICER, but not DROSHA. Biol Reprod. 2014;91:39 pubmed publisher
    Both DICER and DROSHA are RNase III enzymes involved in the biogenesis of small noncoding RNAs...
  22. Wu Q, Song R, Ortogero N, Zheng H, Evanoff R, Small C, et al. The RNase III enzyme DROSHA is essential for microRNA production and spermatogenesis. J Biol Chem. 2012;287:25173-90 pubmed publisher
    DROSHA is a nuclear RNase III enzyme responsible for cleaving primary microRNAs (miRNAs) into precursor miRNAs and thus is essential for the biogenesis of canonical miRNAs...
  23. Kawai S, Amano A. BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex. J Cell Biol. 2012;197:201-8 pubmed publisher
    ..miRNA maturation is controlled by the DROSHA microprocessor complex. However, the detailed mechanism of miRNA biogenesis remains unclear...
  24. Kaneko H, Dridi S, Tarallo V, Gelfand B, Fowler B, Cho W, et al. DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. Nature. 2011;471:325-30 pubmed publisher
    ..These findings reveal a miRNA-independent cell survival function for DICER1 involving retrotransposon transcript degradation, show that Alu RNA can directly cause human pathology, and identify new targets for a major cause of blindness. ..
  25. Andreu Agullo C, Maurin T, Thompson C, Lai E. Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2. Nature. 2011;481:195-8 pubmed publisher
    ..roles in microRNA biogenesis, we unexpectedly observed that Ars2 retains its capacity to promote self-renewal in Drosha and Dicer1 knockout NSCs...
  26. Fan P, Chen Z, Tian P, Liu W, Jiao Y, Xue Y, et al. miRNA biogenesis enzyme Drosha is required for vascular smooth muscle cell survival. PLoS ONE. 2013;8:e60888 pubmed publisher
    miRNA biogenesis enzyme Drosha cleaves double-stranded primary miRNA by interacting with double-stranded RNA binding protein DGCR8 and processes primary miRNA into precursor miRNA to participate in the miRNA biogenesis pathway...
  27. Lugli G, Larson J, Demars M, Smalheiser N. Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain. J Neurochem. 2012;123:459-66 pubmed publisher
    ..b>Drosha and DGCR8 proteins are also expressed in synaptic fractions and PSDs, and are tightly associated with pri-miRs as ..
  28. Yang J, Maurin T, Robine N, Rasmussen K, Jeffrey K, Chandwani R, et al. Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis. Proc Natl Acad Sci U S A. 2010;107:15163-8 pubmed publisher
    ..animal microRNAs (miRNAs) are generated by sequential cleavage of precursor substrates by the Drosha and Dicer RNase III enzymes. Several variant pathways exploit other RNA metabolic activities to generate functional miRNAs...
  29. Zhang C, Long X, Ding Y, Chen X, He J, Liu S, et al. Expression of DROSHA in the Uterus of Mice in Early Pregnancy and Its Potential Significance During Embryo Implantation. Reprod Sci. 2016;23:154-62 pubmed publisher
    ..They play an important role in cell growth and proliferation. DROSHA is the microRNA-processing enzyme and is required for the maturation of microRNAs...
  30. Johanson T, Keown A, Cmero M, Yeo J, Kumar A, Lew A, et al. Drosha controls dendritic cell development by cleaving messenger RNAs encoding inhibitors of myelopoiesis. Nat Immunol. 2015;16:1134-41 pubmed publisher
    ..the microRNA (miRNA) pathway is required for dendritic cell (DC) development, we assessed the effect of ablating Drosha and Dicer, the two enzymes central to miRNA biogenesis...
  31. Yuan S, Schuster A, Tang C, Yu T, Ortogero N, Bao J, et al. Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development. Development. 2016;143:635-47 pubmed publisher
    ..Germline-specific Dicer and Drosha conditional knockout (cKO) mice produce gametes (i.e...
  32. Wan R, Zhou L, Yang H, Zhou Y, Ye S, Zhao Q, et al. Involvement of FMRP in Primary MicroRNA Processing via Enhancing Drosha Translation. Mol Neurobiol. 2017;54:2585-2594 pubmed publisher
    ..Here, we uncover a novel role of FMRP in pri-miRNA processing via controlling Drosha translation...
  33. Zhdanova O, Srivastava S, Di L, Li Z, Tchelebi L, Dworkin S, et al. The inducible deletion of Drosha and microRNAs in mature podocytes results in a collapsing glomerulopathy. Kidney Int. 2011;80:719-30 pubmed publisher
    ..miRNAs play key roles in both the function and differentiation of many cell types. Drosha and Dicer, two RNAase III enzymes, function in a stepwise manner to generate a mature miRNA...
  34. Koay H, Gherardin N, Enders A, Loh L, Mackay L, Almeida C, et al. A three-stage intrathymic development pathway for the mucosal-associated invariant T cell lineage. Nat Immunol. 2016;17:1300-1311 pubmed publisher
    ..Accordingly, this study maps the developmental pathway and checkpoints that control the generation of functional MAIT cells. ..
  35. Coffre M, Benhamou D, Rieß D, Blumenberg L, Snetkova V, Hines M, et al. miRNAs Are Essential for the Regulation of the PI3K/AKT/FOXO Pathway and Receptor Editing during B Cell Maturation. Cell Rep. 2016;17:2271-2285 pubmed publisher
    ..Our analysis revealed that miRNAs are critical for the regulation of the PTEN-AKT-FOXO1 pathway that in turn controls Rag expression during B cell development. ..
  36. Ganesan G, Rao S. A novel noncoding RNA processed by Drosha is restricted to nucleus in mouse. RNA. 2008;14:1399-410 pubmed publisher
    ..Here we report that the mrhl RNA gets processed to a small 80-nucleotide (nt) RNA species and is mediated by the Drosha complex...
  37. Cesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, Chinappi M, et al. A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell. 2011;147:358-69 pubmed publisher
    ..We conclude that the ceRNA network plays an important role in muscle differentiation. ..
  38. Huang V, Place R, Portnoy V, Wang J, Qi Z, Jia Z, et al. Upregulation of Cyclin B1 by miRNA and its implications in cancer. Nucleic Acids Res. 2012;40:1695-707 pubmed publisher
    ..e. Dicer, Drosha, Ago1 and Ago2). In silico analysis identifies highly-complementary sites for 21 miRNAs in the Ccnb1 promoter...
  39. Kuchenreuther M, Weber J. The ARF tumor-suppressor controls Drosha translation to prevent Ras-driven transformation. Oncogene. 2014;33:300-7 pubmed publisher
    ..b>Drosha, the catalytic core of the microprocessor complex, has a critical role in the maturation of specific non-coding ..