Dnmt3b

Summary

Gene Symbol: Dnmt3b
Description: DNA methyltransferase 3B
Alias: MmuIIIB, DNA (cytosine-5)-methyltransferase 3B, DNA MTase MmuIIIB, DNA methyltransferase MmuIIIB
Species: mouse
Products:     Dnmt3b

Top Publications

  1. Ishida C, Ura K, Hirao A, Sasaki H, Toyoda A, Sakaki Y, et al. Genomic organization and promoter analysis of the Dnmt3b gene. Gene. 2003;310:151-9 pubmed
    The Dnmt3b gene encodes a de novo DNA methyltransferase that is essential for normal mouse development. It is highly expressed in early embryos and embryonic stem (ES) cells but downregulated in most adult somatic tissues...
  2. Zuo S, Luo J, Liu M, Xu L, Dong J, Guo W, et al. Suppressing effects of down-regulating DNMT1 and DNMT3b expression on the growth of human cholangiocarcinoma cell line. J Huazhong Univ Sci Technolog Med Sci. 2008;28:276-80 pubmed publisher
    ..In order to explore the roles of two methyltransferase members (DNMT1 and DNMT3b) in the cholangiocarcinoma tumorigenesis, antisense eukaryotic expression plasmid of DNMT1 and DNMT3b gene was ..
  3. Geiman T, Sankpal U, Robertson A, Zhao Y, Zhao Y, Robertson K. DNMT3B interacts with hSNF2H chromatin remodeling enzyme, HDACs 1 and 2, and components of the histone methylation system. Biochem Biophys Res Commun. 2004;318:544-55 pubmed
    ..De novo DNA methyltransferase DNMT3B is critical for embryonic development and is mutated in ICF syndrome...
  4. Lees Murdock D, Shovlin T, Gardiner T, De Felici M, Walsh C. DNA methyltransferase expression in the mouse germ line during periods of de novo methylation. Dev Dyn. 2005;232:992-1002 pubmed
    DNA methyltransferase (DNMT) 3A and DNMT3B are both active de novo DNA methyltransferases required for development, whereas DNMT3L, which has no demonstrable methyltransferase activity, is required for methylation of imprinted genes in ..
  5. Zhu H, Geiman T, Xi S, Jiang Q, Schmidtmann A, Chen T, et al. Lsh is involved in de novo methylation of DNA. EMBO J. 2006;25:335-45 pubmed
    ..Dnmt1 occurring at the replication fork, and de novo methylation established by the methyltransferases Dnmt3a and Dnmt3b. Using an episomal vector in Lsh-/- embryonic fibroblasts, we demonstrate that the acquisition of DNA methylation ..
  6. Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002;277:38746-54 pubmed
    Previous studies have shown that the Dnmt3b gene encodes multiple variants via alternative splicing. However, only one form of Dnmt3a has been identified to date...
  7. Watanabe D, Suetake I, Tada T, Tajima S. Stage- and cell-specific expression of Dnmt3a and Dnmt3b during embryogenesis. Mech Dev. 2002;118:187-90 pubmed
    DNA methylation is essential for development. Two DNA methyltransferases, Dnmt3a and Dnmt3b, contribute to the creation of DNA methylation patterns in embryos...
  8. Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo T, et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 2007;450:908-12 pubmed
    ..The link between hemi-methylated DNA, Np95 and Dnmt1 thus establishes key steps of the mechanism for epigenetic inheritance of DNA methylation. ..
  9. Epsztejn Litman S, Feldman N, Abu Remaileh M, Shufaro Y, Gerson A, Ueda J, et al. De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes. Nat Struct Mol Biol. 2008;15:1176-1183 pubmed publisher
    ..G9a itself is capable of bringing about de novo methylation through its ankyrin domain, by recruiting Dnmt3a and Dnmt3b independently of its histone methyltransferase activity...
  10. Jackson M, Krassowska A, Gilbert N, Chevassut T, Forrester L, Ansell J, et al. Severe global DNA hypomethylation blocks differentiation and induces histone hyperacetylation in embryonic stem cells. Mol Cell Biol. 2004;24:8862-71 pubmed
    ..This demonstrates that successful terminal differentiation is not dependent simply on adequate methylation levels. There is an absolute requirement that the methylation be delivered by the maintenance enzyme Dnmt1. ..

Detail Information

Publications77

  1. Ishida C, Ura K, Hirao A, Sasaki H, Toyoda A, Sakaki Y, et al. Genomic organization and promoter analysis of the Dnmt3b gene. Gene. 2003;310:151-9 pubmed
    The Dnmt3b gene encodes a de novo DNA methyltransferase that is essential for normal mouse development. It is highly expressed in early embryos and embryonic stem (ES) cells but downregulated in most adult somatic tissues...
  2. Zuo S, Luo J, Liu M, Xu L, Dong J, Guo W, et al. Suppressing effects of down-regulating DNMT1 and DNMT3b expression on the growth of human cholangiocarcinoma cell line. J Huazhong Univ Sci Technolog Med Sci. 2008;28:276-80 pubmed publisher
    ..In order to explore the roles of two methyltransferase members (DNMT1 and DNMT3b) in the cholangiocarcinoma tumorigenesis, antisense eukaryotic expression plasmid of DNMT1 and DNMT3b gene was ..
  3. Geiman T, Sankpal U, Robertson A, Zhao Y, Zhao Y, Robertson K. DNMT3B interacts with hSNF2H chromatin remodeling enzyme, HDACs 1 and 2, and components of the histone methylation system. Biochem Biophys Res Commun. 2004;318:544-55 pubmed
    ..De novo DNA methyltransferase DNMT3B is critical for embryonic development and is mutated in ICF syndrome...
  4. Lees Murdock D, Shovlin T, Gardiner T, De Felici M, Walsh C. DNA methyltransferase expression in the mouse germ line during periods of de novo methylation. Dev Dyn. 2005;232:992-1002 pubmed
    DNA methyltransferase (DNMT) 3A and DNMT3B are both active de novo DNA methyltransferases required for development, whereas DNMT3L, which has no demonstrable methyltransferase activity, is required for methylation of imprinted genes in ..
  5. Zhu H, Geiman T, Xi S, Jiang Q, Schmidtmann A, Chen T, et al. Lsh is involved in de novo methylation of DNA. EMBO J. 2006;25:335-45 pubmed
    ..Dnmt1 occurring at the replication fork, and de novo methylation established by the methyltransferases Dnmt3a and Dnmt3b. Using an episomal vector in Lsh-/- embryonic fibroblasts, we demonstrate that the acquisition of DNA methylation ..
  6. Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002;277:38746-54 pubmed
    Previous studies have shown that the Dnmt3b gene encodes multiple variants via alternative splicing. However, only one form of Dnmt3a has been identified to date...
  7. Watanabe D, Suetake I, Tada T, Tajima S. Stage- and cell-specific expression of Dnmt3a and Dnmt3b during embryogenesis. Mech Dev. 2002;118:187-90 pubmed
    DNA methylation is essential for development. Two DNA methyltransferases, Dnmt3a and Dnmt3b, contribute to the creation of DNA methylation patterns in embryos...
  8. Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo T, et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 2007;450:908-12 pubmed
    ..The link between hemi-methylated DNA, Np95 and Dnmt1 thus establishes key steps of the mechanism for epigenetic inheritance of DNA methylation. ..
  9. Epsztejn Litman S, Feldman N, Abu Remaileh M, Shufaro Y, Gerson A, Ueda J, et al. De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes. Nat Struct Mol Biol. 2008;15:1176-1183 pubmed publisher
    ..G9a itself is capable of bringing about de novo methylation through its ankyrin domain, by recruiting Dnmt3a and Dnmt3b independently of its histone methyltransferase activity...
  10. Jackson M, Krassowska A, Gilbert N, Chevassut T, Forrester L, Ansell J, et al. Severe global DNA hypomethylation blocks differentiation and induces histone hyperacetylation in embryonic stem cells. Mol Cell Biol. 2004;24:8862-71 pubmed
    ..This demonstrates that successful terminal differentiation is not dependent simply on adequate methylation levels. There is an absolute requirement that the methylation be delivered by the maintenance enzyme Dnmt1. ..
  11. Watanabe D, Suetake I, Tajima S, Hanaoka K. Expression of Dnmt3b in mouse hematopoietic progenitor cells and spermatogonia at specific stages. Gene Expr Patterns. 2004;5:43-9 pubmed
    Two de novo-type DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation of DNA methylation patterns during development...
  12. Linhart H, Lin H, Yamada Y, Moran E, Steine E, Gokhale S, et al. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007;21:3110-22 pubmed
  13. Kaneda M, Hirasawa R, Chiba H, Okano M, Li E, Sasaki H. Genetic evidence for Dnmt3a-dependent imprinting during oocyte growth obtained by conditional knockout with Zp3-Cre and complete exclusion of Dnmt3b by chimera formation. Genes Cells. 2010;15:169-79 pubmed publisher
    In the male and female germ-lines of mice, both of the two de novo DNA methyltransferases Dnmt3a and Dnmt3b are expressed...
  14. Lorincz M, Schübeler D, Hutchinson S, Dickerson D, Groudine M. DNA methylation density influences the stability of an epigenetic imprint and Dnmt3a/b-independent de novo methylation. Mol Cell Biol. 2002;22:7572-80 pubmed
    ..The DNA methyltransferases (MTases) Dnmt3a and Dnmt3b are thought to be the sole de novo MTases in the mammalian genome...
  15. Karimi M, Goyal P, Maksakova I, Bilenky M, Leung D, Tang J, et al. DNA methylation and SETDB1/H3K9me3 regulate predominantly distinct sets of genes, retroelements, and chimeric transcripts in mESCs. Cell Stem Cell. 2011;8:676-87 pubmed publisher
    ..Setdb1 are distinct from those derepressed in mESC deficient in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b, with the exception of a small number of primarily germline-specific genes...
  16. Ooi S, Qiu C, Bernstein E, Li K, Jia D, Yang Z, et al. DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA. Nature. 2007;448:714-7 pubmed
    ..an enzymatically inactive regulatory factor that is related in sequence to the DNA methyltransferases DNMT3A and DNMT3B. The main proteins that interact in vivo with the product of an epitope-tagged allele of the endogenous Dnmt3L ..
  17. Shah M, Vasanthakumar A, Barnes N, Figueroa M, Kamp A, Hendrick C, et al. DNMT3B7, a truncated DNMT3B isoform expressed in human tumors, disrupts embryonic development and accelerates lymphomagenesis. Cancer Res. 2010;70:5840-50 pubmed publisher
    ..To test if a truncated DNMT3B isoform disrupts DNA methylation in vivo, we constructed two lines of transgenic mice expressing DNMT3B7, a ..
  18. Qiu C, Sawada K, Zhang X, Cheng X. The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds. Nat Struct Biol. 2002;9:217-24 pubmed
    ..sequence motif found in > 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b. These proteins often contain other chromatin-association domains...
  19. Oka M, Rodic N, Graddy J, Chang L, Terada N. CpG sites preferentially methylated by Dnmt3a in vivo. J Biol Chem. 2006;281:9901-8 pubmed
    Dnmt3a and Dnmt3b are two major de novo DNA methyltransferases essential for embryonic development in mammals...
  20. Chen T, Tsujimoto N, Li E. The PWWP domain of Dnmt3a and Dnmt3b is required for directing DNA methylation to the major satellite repeats at pericentric heterochromatin. Mol Cell Biol. 2004;24:9048-58 pubmed
    Dnmt3a and Dnmt3b are responsible for the establishment of DNA methylation patterns during development...
  21. Dodge J, Okano M, Dick F, Tsujimoto N, Chen T, Wang S, et al. Inactivation of Dnmt3b in mouse embryonic fibroblasts results in DNA hypomethylation, chromosomal instability, and spontaneous immortalization. J Biol Chem. 2005;280:17986-91 pubmed
    ..In this study, we have investigated how targeted disruption of the DNA methyltransferases Dnmt3a and Dnmt3b affects the growth of mouse embryonic fibroblasts (MEFs). Our studies led to the following observations...
  22. Borgel J, Guibert S, Li Y, Chiba H, Schübeler D, Sasaki H, et al. Targets and dynamics of promoter DNA methylation during early mouse development. Nat Genet. 2010;42:1093-100 pubmed publisher
    ..De novo methylation during early embryogenesis is catalyzed by Dnmt3b, and absence of DNA methylation leads to ectopic gene activation in the embryo...
  23. Kaneda M, Okano M, Hata K, Sado T, Tsujimoto N, Li E, et al. Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature. 2004;429:900-3 pubmed
    ..novo DNA methyltransferases of the Dnmt3 family are implicated in maternal imprinting, the lethality of Dnmt3a and Dnmt3b knockout mice has precluded further studies...
  24. Lucifero D, Mann M, Bartolomei M, Trasler J. Gene-specific timing and epigenetic memory in oocyte imprinting. Hum Mol Genet. 2004;13:839-49 pubmed
    ..methylation acquisition was probably related to oocyte diameter and coincided with the accumulation of Dnmt3a, Dnmt3b and Dnmt3L transcripts...
  25. Oka M, Meacham A, Hamazaki T, Rodic N, Chang L, Terada N. De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine. Oncogene. 2005;24:3091-9 pubmed
    ..Of interest, Dnmt3a-Dnmt3b double null ES cells were highly resistant to 5-aza-dC when compared to wild type, Dnmt3a null, Dnmt3b null, or ..
  26. Okano M, Bell D, Haber D, Li E. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 1999;99:247-57 pubmed
    ..Here we demonstrate that two recently identified DNA methyltransferases, Dnmt3a and Dnmt3b, are essential for de novo methylation and for mouse development...
  27. Li J, Pu M, Hirasawa R, Li B, Huang Y, Zeng R, et al. Synergistic function of DNA methyltransferases Dnmt3a and Dnmt3b in the methylation of Oct4 and Nanog. Mol Cell Biol. 2007;27:8748-59 pubmed
    DNA methylation plays an important role in gene silencing in mammals. Two de novo methyltransferases, Dnmt3a and Dnmt3b, are required for the establishment of genomic methylation patterns in development...
  28. Gonzalo S, Jaco I, Fraga M, Chen T, Li E, Esteller M, et al. DNA methyltransferases control telomere length and telomere recombination in mammalian cells. Nat Cell Biol. 2006;8:416-24 pubmed
    ..Mouse embryonic stem (ES) cells genetically deficient for DNMT1, or both DNMT3a and DNMT3b have dramatically elongated telomeres compared with wild-type controls...
  29. Ueda Y, Okano M, Williams C, Chen T, Georgopoulos K, Li E. Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome. Development. 2006;133:1183-92 pubmed
    ..anomalies) syndrome is a rare autosomal recessive disease caused by mutations in the DNA methyltransferase gene DNMT3B. To investigate the function of Dnmt3b in mouse development and to create animal models for ICF syndrome, we have ..
  30. Hirasawa R, Chiba H, Kaneda M, Tajima S, Li E, Jaenisch R, et al. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 2008;22:1607-16 pubmed publisher
    ..Here we demonstrate, by using conditional knockout mice, that the other known DNA methyltransferases Dnmt3a and Dnmt3b are dispensable for the maintenance of the methylation marks at most imprinted loci...
  31. Kato Y, Kaneda M, Hata K, Kumaki K, Hisano M, Kohara Y, et al. Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse. Hum Mol Genet. 2007;16:2272-80 pubmed
    ..Furthermore, we have examined the roles of the de novo methyltransferases (Dnmt3a and Dnmt3b) and related protein (Dnmt3L) in this process...
  32. Brenner C, Deplus R, Didelot C, Loriot A, Viré E, De Smet C, et al. Myc represses transcription through recruitment of DNA methyltransferase corepressor. EMBO J. 2005;24:336-46 pubmed
    ..Furthermore, these findings suggest that targeting of DNA methyltransferases by transcription factors is a wide and general mechanism for the generation of specific DNA methylation patterns within a cell. ..
  33. Sakai Y, Suetake I, Shinozaki F, Yamashina S, Tajima S. Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos. Gene Expr Patterns. 2004;5:231-7 pubmed
    ..In the present study, we examined which de novo-type DNA methyltransferase, Dnmt3a, Dnmt3a2 or Dnmt3b is expressed in gonocytes at these stages...
  34. Suetake I, Miyazaki J, Murakami C, Takeshima H, Tajima S. Distinct enzymatic properties of recombinant mouse DNA methyltransferases Dnmt3a and Dnmt3b. J Biochem. 2003;133:737-44 pubmed
    Recombinant mouse Dnmt3a and Dnmt3b were expressed in sf9 cells and purified to near homogeneity. The purified Dnmt3a and Dnmt3b gave specific activities of 1.8 +/- 0.3 and 1.3 +/- 0...
  35. Gowher H, Liebert K, Hermann A, Xu G, Jeltsch A. Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L. J Biol Chem. 2005;280:13341-8 pubmed
    ..We show here that Dnmt3L stimulates the catalytic activity of the Dnmt3A and Dnmt3B enzymes by directly binding to their respective catalytic domains via its own C-terminal domain...
  36. Chen T, Ueda Y, Dodge J, Wang Z, Li E. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol. 2003;23:5594-605 pubmed
    We have previously shown that the DNA methyltransferases Dnmt3a and Dnmt3b carry out de novo methylation of the mouse genome during early postimplantation development and of maternally imprinted genes in the oocyte...
  37. Hata K, Okano M, Lei H, Li E. Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice. Development. 2002;129:1983-93 pubmed
    ..In this study, we demonstrate that Dnmt3L, a protein sharing homology with DNA methyltransferases, Dnmt3a and Dnmt3b, but lacking enzymatic activity, is essential for the establishment of maternal methylation imprints and ..
  38. Lucifero D, La Salle S, Bourc his D, Martel J, Bestor T, Trasler J. Coordinate regulation of DNA methyltransferase expression during oogenesis. BMC Dev Biol. 2007;7:36 pubmed
    ..Here we report the expression dynamics of Dnmt3a and Dnmt3b, as well as a regulator of DNA methylation, Dnmt3L, in isolated female germ cells...
  39. Gowher H, Jeltsch A. Molecular enzymology of the catalytic domains of the Dnmt3a and Dnmt3b DNA methyltransferases. J Biol Chem. 2002;277:20409-14 pubmed
    The C-terminal domains of the mammalian DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b harbor all the conserved motifs characteristic for cytosine-C5 methyltransferases...
  40. Hirasawa R, Sasaki H. Dynamic transition of Dnmt3b expression in mouse pre- and early post-implantation embryos. Gene Expr Patterns. 2009;9:27-30 pubmed publisher
    The de novo DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation of DNA methylation patterns in mouse development...
  41. Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells. 2006;11:805-14 pubmed
    DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b cooperatively regulate cytosine methylation in CpG dinucleotides in mammalian genomes, providing an epigenetic basis for gene silencing and maintenance of genome integrity...
  42. Arand J, Spieler D, Karius T, Branco M, Meilinger D, Meissner A, et al. In vivo control of CpG and non-CpG DNA methylation by DNA methyltransferases. PLoS Genet. 2012;8:e1002750 pubmed publisher
    ..Moreover, non-CpG cytosine methylation is confined to ESCs and exclusively catalysed by Dnmt3a and Dnmt3b. This sequence position-, cell type-, and region-dependent non-CpG methylation is strongly linked to neighboring ..
  43. La Salle S, Mertineit C, Taketo T, Moens P, Bestor T, Trasler J. Windows for sex-specific methylation marked by DNA methyltransferase expression profiles in mouse germ cells. Dev Biol. 2004;268:403-15 pubmed
    ..We compared the temporal expression patterns of the DNA methyltransferases, DNMT1, DNMT3a, DNMT3b, and DNMT3l in the male and female germ lines...
  44. Gopalakrishnan S, Sullivan B, Trazzi S, Della Valle G, Robertson K. DNMT3B interacts with constitutive centromere protein CENP-C to modulate DNA methylation and the histone code at centromeric regions. Hum Mol Genet. 2009;18:3178-93 pubmed publisher
    ..methylation patterns are mediated by the combined action of three DNA methyltransferases: DNMT1, DNMT3A and DNMT3B. Compelling links exist between DNMT3B and chromosome stability as emphasized by the mitotic defects that are a ..
  45. Okano M, Xie S, Li E. Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nat Genet. 1998;19:219-20 pubmed
  46. Xie Z, Huang Y, Chen Z, Riggs A, Ding J, Gowher H, et al. Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L. Hum Mol Genet. 2006;15:1375-85 pubmed
    Deficiency in DNA methyltransferase DNMT3B causes a recessive human disorder characterized by immunodeficiency, centromeric instability and facial anomalies (ICF) in association with defects in genomic methylation...
  47. La Salle S, Trasler J. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol. 2006;296:71-82 pubmed
    ..We compared the temporal expression patterns of the postulated de novo DNA methyltransferases DNMT3a and DNMT3b in murine male germ cells...
  48. Ge Y, Pu M, Gowher H, Wu H, Ding J, Jeltsch A, et al. Chromatin targeting of de novo DNA methyltransferases by the PWWP domain. J Biol Chem. 2004;279:25447-54 pubmed
    ..Two de novo DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the process...
  49. Ratnam S, Engler P, Bozek G, Mao L, Podlutsky A, Austad S, et al. Identification of Ssm1b, a novel modifier of DNA methylation, and its expression during mouse embryogenesis. Development. 2014;141:2024-34 pubmed publisher
    ..Methylation during B6 embryonic development depends on Dnmt3b but not Mecp2...
  50. Bai S, Ghoshal K, Datta J, Majumder S, Yoon S, Jacob S. DNA methyltransferase 3b regulates nerve growth factor-induced differentiation of PC12 cells by recruiting histone deacetylase 2. Mol Cell Biol. 2005;25:751-66 pubmed
    ..The mRNA and protein levels of de novo methyltransferase Dnmt3b increased, whereas those of Dnmt3a and Dnmt1 decreased, during NGF-induced neurite outgrowth...
  51. Horii T, Suetake I, Yanagisawa E, Morita S, Kimura M, Nagao Y, et al. The Dnmt3b splice variant is specifically expressed in in vitro-manipulated blastocysts and their derivative ES cells. J Reprod Dev. 2011;57:579-85 pubmed
    ..These DNA methylation patterns are established and maintained by three DNA methyltransferases: Dnmt1, Dnmt3a and Dnmt3b. Dnmt3b is responsible for the creation of methylation patterns during the early stage of embryogenesis and ..
  52. Nührenberg T, Hammann N, Schnick T, Preißl S, Witten A, Stoll M, et al. Cardiac Myocyte De Novo DNA Methyltransferases 3a/3b Are Dispensable for Cardiac Function and Remodeling after Chronic Pressure Overload in Mice. PLoS ONE. 2015;10:e0131019 pubmed publisher
    ..Here, we tested whether cardiomyocyte-specific loss of de novo DNA methyltransferases Dnmt3a and Dnmt3b altered cardiac function and remodeling after chronic left ventricular pressure overload...
  53. Meilinger D, Fellinger K, Bultmann S, Rothbauer U, Bonapace I, Klinkert W, et al. Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells. EMBO Rep. 2009;10:1259-64 pubmed publisher
    ..we show that Np95 interacts more strongly with regulatory domains of the de novo methyltransferases Dnmt3a and Dnmt3b. To investigate possible functions, we developed an epigenetic silencing assay using fluorescent reporters in ..
  54. Feng J, Chang H, Li E, Fan G. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. J Neurosci Res. 2005;79:734-46 pubmed
    ..of DNA methylation in the brain, we examined the expression pattern of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the mouse central nervous system (CNS)...
  55. Zhubi A, Veldic M, Puri N, Kadriu B, Caruncho H, Loza I, et al. An upregulation of DNA-methyltransferase 1 and 3a expressed in telencephalic GABAergic neurons of schizophrenia patients is also detected in peripheral blood lymphocytes. Schizophr Res. 2009;111:115-22 pubmed publisher
    ..Hence, PBL studies may become useful to investigate a diagnostic epigenetic marker of SZ morbidity. ..
  56. Lal G, Zhang N, van der Touw W, Ding Y, Ju W, Bottinger E, et al. Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation. J Immunol. 2009;182:259-73 pubmed
    ..CD4(+) T cells, activated CD4(+) T cells, and peripheral TGFbeta-induced Treg in which it was bound by DNMT1, DNMT3b, MeCP2, and MBD2...
  57. King A, Huang K, Rubbi L, Liu S, Wang C, Wang Y, et al. Reversible Regulation of Promoter and Enhancer Histone Landscape by DNA Methylation in Mouse Embryonic Stem Cells. Cell Rep. 2016;17:289-302 pubmed publisher
    ..Collectively, our data show that DNA methylation is essential and instructive for deposition of specific histone modifications across regulatory regions, which together influences gene expression patterns in mESCs. ..
  58. Youngson N, Epp T, Roberts A, Daxinger L, Ashe A, Huang E, et al. No evidence for cumulative effects in a Dnmt3b hypomorph across multiple generations. Mamm Genome. 2013;24:206-17 pubmed publisher
    ..This suggests that in our model the abnormalities are reset each generation and the processes of transgenerational epigenetic reprogramming are effective in preventing their inheritance. ..
  59. Yang S, Kim B, NORWOOD TORO L, Skoultchi A. H1 linker histone promotes epigenetic silencing by regulating both DNA methylation and histone H3 methylation. Proc Natl Acad Sci U S A. 2013;110:1708-13 pubmed publisher
    ..Some, but not all, murine H1 subtypes interact with DNA methyltransferases DNMT1 and DNMT3B. The interactions are direct and require a portion of the H1 C-terminal domain...
  60. MacDonald J, Gin C, Roskams A. Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development. Dev Biol. 2005;288:461-73 pubmed
    ..DNMT1 is induced in cycling progenitors and is retained in post-mitotic olfactory receptor neurons (ORNs). DNMT3b is restricted to mitotic olfactory progenitors, whereas DNMT3a is expressed only in post-mitotic immature neurons ..
  61. Hattori N, Abe T, Hattori N, Suzuki M, Matsuyama T, Yoshida S, et al. Preference of DNA methyltransferases for CpG islands in mouse embryonic stem cells. Genome Res. 2004;14:1733-40 pubmed
    ..components, we investigated the genome-wide DNA methylation pattern of the T-DMRs with Dnmt1-, Dnmt3a-, and/or Dnmt3b-deficient ES cells by restriction landmark genomic scanning (RLGS)...
  62. Raddatz G, Guzzardo P, Olova N, Fantappié M, Rampp M, Schaefer M, et al. Dnmt2-dependent methylomes lack defined DNA methylation patterns. Proc Natl Acad Sci U S A. 2013;110:8627-31 pubmed publisher
    ..Our results thus uncover fundamental differences among animal methylomes and suggest that DNA methylation is dispensable for a considerable number of eukaryotic organisms...
  63. Zhang D, Wu B, Wang P, Wang Y, Lu P, Nechiporuk T, et al. Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts. Nucleic Acids Res. 2017;45:3102-3115 pubmed publisher
    ..This process is dependent on DNA methyltransferase 3B (DNMT3B) and leads to suppression of adult cardiac genes in developing hearts...
  64. Peters S, Hlady R, Opavska J, Klinkebiel D, Pirruccello S, Talmon G, et al. Tumor suppressor functions of Dnmt3a and Dnmt3b in the prevention of malignant mouse lymphopoiesis. Leukemia. 2014;28:1138-42 pubmed publisher
  65. Hu Y, Hirasawa R, Hu J, Hata K, Li C, Jin Y, et al. Regulation of DNA methylation activity through Dnmt3L promoter methylation by Dnmt3 enzymes in embryonic development. Hum Mol Genet. 2008;17:2654-64 pubmed publisher
    The genomic DNA is methylated by de novo methyltransferases Dnmt3a and Dnmt3b during early embryonic development. The establishment of appropriate methylation patterns depends on a fine regulation of the methyltransferase activity...
  66. Craig J, Earle E, Canham P, Wong L, Anderson M, Choo K. Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns. Hum Mol Genet. 2003;12:3109-21 pubmed
    ..regions; and (b) heterochromatin-associated proteins (MeCP2, MBD1, MBD2, ATRX, HP1alpha, HDAC1, HDAC2, DNMT1 and DNMT3b), which colocalize with centromeric/pericentric heterochromatin and all other major heterochromatic sites...
  67. Boland M, Christman J. Characterization of Dnmt3b:thymine-DNA glycosylase interaction and stimulation of thymine glycosylase-mediated repair by DNA methyltransferase(s) and RNA. J Mol Biol. 2008;379:492-504 pubmed publisher
    ..patterns are established and maintained at CpG dinucleotides by DNA methyltransferases (Dnmt1, Dnmt3a, and Dnmt3b). In mammals and many other eukaryotes, the CpG dinucleotide is underrepresented in the genome...
  68. Oda M, Oxley D, Dean W, Reik W. Regulation of lineage specific DNA hypomethylation in mouse trophectoderm. PLoS ONE. 2013;8:e68846 pubmed publisher
    ..lineage cells re-acquire a highly methylated genome dependent on the DNA methyltransferases (DNMTs) Dnmt3a and Dnmt3b that are required for de novo methylation...
  69. Pawlak M, Jaenisch R. De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state. Genes Dev. 2011;25:1035-40 pubmed publisher
    ..studies, we generated iPSCs from fibroblasts that were deficient in de novo DNA methylation mediated by Dnmt3a and Dnmt3b. These iPSCs reactivated pluripotency genes, underwent self-renewal, and showed restricted developmental potential ..
  70. Tajima S, Suetake I, Takeshita K, Nakagawa A, Kimura H. Domain Structure of the Dnmt1, Dnmt3a, and Dnmt3b DNA Methyltransferases. Adv Exp Med Biol. 2016;945:63-86 pubmed
    In mammals, three DNA methyltransferases, Dnmt1, Dnmt3a, and Dnmt3b, have been identified...
  71. Kim I, Lee M, Park K, Jeon Y, Park J, Hwang E, et al. Roles of Mis18? in epigenetic regulation of centromeric chromatin and CENP-A loading. Mol Cell. 2012;46:260-73 pubmed publisher
    ..Together, our findings uncover the functional mechanism of Mis18? and its pivotal role in mammalian cell cycle...
  72. Yu Z, Kong Q, Kone B. Aldosterone reprograms promoter methylation to regulate ?ENaC transcription in the collecting duct. Am J Physiol Renal Physiol. 2013;305:F1006-13 pubmed publisher
    ..5-Aza-CdR treatment or small interfering RNA-mediated knockdown of DNMT3b or methyl-CpG-binding domain protein (MBD)-4 derepressed basal ?ENaC transcription, indicating that promoter ..
  73. Cheng C, Wang S, Ye P, Huang X, Liu Z, Wu J, et al. 'Default' generated neonatal regulatory T cells are hypomethylated at conserved non-coding sequence 2 and promote long-term cardiac allograft survival. Immunology. 2014;143:618-30 pubmed publisher
    ..Due to their involvement in Foxp3 regulation, we examined the role of DNA methyltransferase 1 (DNMT1) and DNMT3b during the induction of neoTreg cells in the Foxp3(gfp) mice...
  74. Umemura Y, Koike N, Matsumoto T, Yoo S, Chen Z, Yasuhara N, et al. Transcriptional program of Kpna2/Importin-α2 regulates cellular differentiation-coupled circadian clock development in mammalian cells. Proc Natl Acad Sci U S A. 2014;111:E5039-48 pubmed publisher
    ..These results suggest that the programmed gene expression network regulates the differentiation-coupled circadian clock development in mammalian cells, at least in part via posttranscriptional regulation of clock proteins. ..
  75. Haney S, Hlady R, Opavska J, Klinkebiel D, Pirruccello S, Dutta S, et al. Methylation-independent repression of Dnmt3b contributes to oncogenic activity of Dnmt3a in mouse MYC-induced T-cell lymphomagenesis. Oncogene. 2015;34:5436-5446 pubmed publisher
    ..pathway analysis identified upregulation of 17 putative tumor suppressor genes, including DNA methyltransferase Dnmt3b, in Dnmt3a-deficient lymphomas as molecular events potentially responsible for the delayed lymphomagenesis in ..
  76. May A, Kirchner R, Muller H, Hartmann P, El Hajj N, Tresch A, et al. Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres. Biol Reprod. 2009;80:194-202 pubmed publisher
    ..Immunolocalization of DNMT3A, MBD3, APEX1, and LIG3 in most or all nuclei of 40-60-cell embryos is a good indicator of functional activity of genes that are activated by the 16-cell stage...
  77. Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, et al. The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. Dev Cell. 2009;17:775-87 pubmed publisher