Dnmt1

Summary

Gene Symbol: Dnmt1
Description: DNA methyltransferase (cytosine-5) 1
Alias: Cxxc9, Dnmt, Dnmt1o, MCMT, MTase, Met-1, Met1, MommeD2, m.MmuI, DNA (cytosine-5)-methyltransferase 1, DNA MTase MmuI, DNA methyltransferase MmuI
Species: mouse
Products:     Dnmt1

Top Publications

  1. Li E, Bestor T, Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell. 1992;69:915-26 pubmed
  2. Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells. 2006;11:805-14 pubmed
    DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b cooperatively regulate cytosine methylation in CpG dinucleotides in mammalian genomes, providing an epigenetic basis for gene silencing and maintenance of genome integrity...
  3. Cirio M, Martel J, Mann M, Toppings M, Bartolomei M, Trasler J, et al. DNA methyltransferase 1o functions during preimplantation development to preclude a profound level of epigenetic variation. Dev Biol. 2008;324:139-50 pubmed publisher
    Most mouse embryos developing in the absence of the oocyte-derived DNA methyltransferase 1o (DNMT1o-deficient embryos) have significant delays in development and a wide range of anatomical abnormalities...
  4. Nasonkin I, Lazo K, Hambright D, Brooks M, Fariss R, Swaroop A. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol. 2011;519:1914-30 pubmed publisher
    DNA methyltransferases--DNMT1, DNMT3a, and DNMT3b--produce methylation patterns that dynamically regulate chromatin remodeling and gene expression...
  5. Goll M, Kirpekar F, Maggert K, Yoder J, Hsieh C, Zhang X, et al. Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2. Science. 2006;311:395-8 pubmed
    ..Indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a Dnmt2-like RNA methyltransferase. ..
  6. Blewitt M, Vickaryous N, Hemley S, Ashe A, Bruxner T, Preis J, et al. An N-ethyl-N-nitrosourea screen for genes involved in variegation in the mouse. Proc Natl Acad Sci U S A. 2005;102:7629-34 pubmed
    ..The study demonstrates the power of sensitized screens in the mouse not only for the discovery of novel genes involved in a particular process but also for the elucidation of the biology of that process. ..
  7. Gowher H, Stockdale C, Goyal R, Ferreira H, Owen Hughes T, Jeltsch A. De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferases. Biochemistry. 2005;44:9899-904 pubmed
    ..We investigated de novo methylation of nucleosomal DNA in vitro and show that the Dnmt3a and Dnmt1 DNA methyltransferases efficiently methylate nucleosomal DNA without dissociation of the histone octamer from the ..
  8. Easwaran H, Schermelleh L, Leonhardt H, Cardoso M. Replication-independent chromatin loading of Dnmt1 during G2 and M phases. EMBO Rep. 2004;5:1181-6 pubmed
    The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand...
  9. Tucker K, Talbot D, Lee M, Leonhardt H, Jaenisch R. Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene. Proc Natl Acad Sci U S A. 1996;93:12920-5 pubmed
    ..Our results define an amino-terminal domain of the mammalian MTase that is crucial for stable expression and function in vivo.
  10. Suetake I, Hayata D, Tajima S. The amino-terminus of mouse DNA methyltransferase 1 forms an independent domain and binds to DNA with the sequence involving PCNA binding motif. J Biochem. 2006;140:763-76 pubmed
    DNA methylation patterns in genome are maintained during replication by a DNA methyltransferase Dnmt1. Mouse Dnmt1 is a 180 kDa protein comprising the N-terminal regulatory domain, which covers 2/3 of the molecule, and the rest C-..

Detail Information

Publications81

  1. Li E, Bestor T, Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell. 1992;69:915-26 pubmed
  2. Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells. 2006;11:805-14 pubmed
    DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b cooperatively regulate cytosine methylation in CpG dinucleotides in mammalian genomes, providing an epigenetic basis for gene silencing and maintenance of genome integrity...
  3. Cirio M, Martel J, Mann M, Toppings M, Bartolomei M, Trasler J, et al. DNA methyltransferase 1o functions during preimplantation development to preclude a profound level of epigenetic variation. Dev Biol. 2008;324:139-50 pubmed publisher
    Most mouse embryos developing in the absence of the oocyte-derived DNA methyltransferase 1o (DNMT1o-deficient embryos) have significant delays in development and a wide range of anatomical abnormalities...
  4. Nasonkin I, Lazo K, Hambright D, Brooks M, Fariss R, Swaroop A. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol. 2011;519:1914-30 pubmed publisher
    DNA methyltransferases--DNMT1, DNMT3a, and DNMT3b--produce methylation patterns that dynamically regulate chromatin remodeling and gene expression...
  5. Goll M, Kirpekar F, Maggert K, Yoder J, Hsieh C, Zhang X, et al. Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2. Science. 2006;311:395-8 pubmed
    ..Indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a Dnmt2-like RNA methyltransferase. ..
  6. Blewitt M, Vickaryous N, Hemley S, Ashe A, Bruxner T, Preis J, et al. An N-ethyl-N-nitrosourea screen for genes involved in variegation in the mouse. Proc Natl Acad Sci U S A. 2005;102:7629-34 pubmed
    ..The study demonstrates the power of sensitized screens in the mouse not only for the discovery of novel genes involved in a particular process but also for the elucidation of the biology of that process. ..
  7. Gowher H, Stockdale C, Goyal R, Ferreira H, Owen Hughes T, Jeltsch A. De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferases. Biochemistry. 2005;44:9899-904 pubmed
    ..We investigated de novo methylation of nucleosomal DNA in vitro and show that the Dnmt3a and Dnmt1 DNA methyltransferases efficiently methylate nucleosomal DNA without dissociation of the histone octamer from the ..
  8. Easwaran H, Schermelleh L, Leonhardt H, Cardoso M. Replication-independent chromatin loading of Dnmt1 during G2 and M phases. EMBO Rep. 2004;5:1181-6 pubmed
    The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand...
  9. Tucker K, Talbot D, Lee M, Leonhardt H, Jaenisch R. Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene. Proc Natl Acad Sci U S A. 1996;93:12920-5 pubmed
    ..Our results define an amino-terminal domain of the mammalian MTase that is crucial for stable expression and function in vivo.
  10. Suetake I, Hayata D, Tajima S. The amino-terminus of mouse DNA methyltransferase 1 forms an independent domain and binds to DNA with the sequence involving PCNA binding motif. J Biochem. 2006;140:763-76 pubmed
    DNA methylation patterns in genome are maintained during replication by a DNA methyltransferase Dnmt1. Mouse Dnmt1 is a 180 kDa protein comprising the N-terminal regulatory domain, which covers 2/3 of the molecule, and the rest C-..
  11. Feng J, Zhou Y, Campbell S, Le T, Li E, Sweatt J, et al. Dnmt1 and Dnmt3a maintain DNA methylation and regulate synaptic function in adult forebrain neurons. Nat Neurosci. 2010;13:423-30 pubmed publisher
    b>Dnmt1 and Dnmt3a are important DNA methyltransferases that are expressed in postmitotic neurons, but their function in the CNS is unclear...
  12. Fatemi M, Hermann A, Pradhan S, Jeltsch A. The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA. J Mol Biol. 2001;309:1189-99 pubmed
    The mammalian DNA methyltransferase Dnmt1 is responsible for the maintenance of the pattern of DNA methylation in vivo. It is a large multidomain enzyme comprising 1620 amino acid residues...
  13. La Salle S, Mertineit C, Taketo T, Moens P, Bestor T, Trasler J. Windows for sex-specific methylation marked by DNA methyltransferase expression profiles in mouse germ cells. Dev Biol. 2004;268:403-15 pubmed
    ..We compared the temporal expression patterns of the DNA methyltransferases, DNMT1, DNMT3a, DNMT3b, and DNMT3l in the male and female germ lines...
  14. Wang J, Hevi S, Kurash J, Lei H, Gay F, Bajko J, et al. The lysine demethylase LSD1 (KDM1) is required for maintenance of global DNA methylation. Nat Genet. 2009;41:125-9 pubmed publisher
    ..This loss correlates with a decrease in DNA methyltransferase 1 (Dnmt1) protein, as a result of reduced Dnmt1 stability...
  15. Grohmann M, Spada F, Schermelleh L, Alenina N, Bader M, Cardoso M, et al. Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming. BMC Dev Biol. 2005;5:18 pubmed
    ..Using GFP fusions we show that both the long and short isoforms of Dnmt1 localize to the nucleus of somatic cells and the cytoplasm of preimplantation embryos and that these subcellular ..
  16. Frauer C, Rottach A, Meilinger D, Bultmann S, Fellinger K, Hasenöder S, et al. Different binding properties and function of CXXC zinc finger domains in Dnmt1 and Tet1. PLoS ONE. 2011;6:e16627 pubmed publisher
    ..We compared the structure and function of the CXXC domains in the DNA methyltransferase Dnmt1 and the methylcytosine dioxygenase Tet1...
  17. Bostick M, Kim J, Esteve P, Clark A, Pradhan S, Jacobsen S. UHRF1 plays a role in maintaining DNA methylation in mammalian cells. Science. 2007;317:1760-4 pubmed
    ..UHRF1 colocalizes with the maintenance DNA methyltransferase protein DNMT1 throughout S phase. UHRF1 appears to tether DNMT1 to chromatin through its direct interaction with DNMT1...
  18. Eden A, Gaudet F, Waghmare A, Jaenisch R. Chromosomal instability and tumors promoted by DNA hypomethylation. Science. 2003;300:455 pubmed
  19. Mortusewicz O, Schermelleh L, Walter J, Cardoso M, Leonhardt H. Recruitment of DNA methyltransferase I to DNA repair sites. Proc Natl Acad Sci U S A. 2005;102:8905-9 pubmed
    ..system to introduce DNA lesions at defined subnuclear sites, we tested whether the major DNA methyltransferase (Dnmt1) or one of the two de novo methyltransferases (Dnmt3a, Dnmt3b) are recruited to sites of DNA repair in vivo...
  20. Toppings M, Castro C, Mills P, Reinhart B, Schatten G, Ahrens E, et al. Profound phenotypic variation among mice deficient in the maintenance of genomic imprints. Hum Reprod. 2008;23:807-18 pubmed publisher
    ..of maintenance methylation at one specific stage of preimplantation, induced by a deficiency of the oocyte-derived Dnmt1o protein and known to produce biallelic expression of imprinted genes...
  21. Margot J, Aguirre Arteta A, Di Giacco B, Pradhan S, Roberts R, Cardoso M, et al. Structure and function of the mouse DNA methyltransferase gene: Dnmt1 shows a tripartite structure. J Mol Biol. 2000;297:293-300 pubmed
    Dnmt1 is the predominant DNA methyltransferase (MTase) in mammals...
  22. Fan G, Beard C, Chen R, Csankovszki G, Sun Y, Siniaia M, et al. DNA hypomethylation perturbs the function and survival of CNS neurons in postnatal animals. J Neurosci. 2001;21:788-97 pubmed
    DNA methyltransferase I (Dnmt1), the maintenance enzyme for DNA cytosine methylation, is expressed at high levels in the CNS during embryogenesis and after birth...
  23. Chong S, Vickaryous N, Ashe A, Zamudio N, Youngson N, Hemley S, et al. Modifiers of epigenetic reprogramming show paternal effects in the mouse. Nat Genet. 2007;39:614-22 pubmed
    ..Paternal effects of this type have not been reported previously in mammals and suggest that the untransmitted genotype of male parents can influence the phenotype of their offspring. ..
  24. Dion V, Lin Y, Hubert L, Waterland R, Wilson J. Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline. Hum Mol Genet. 2008;17:1306-17 pubmed publisher
    ..Here, we show that the maintenance DNA methyltransferase Dnmt1, which preserves the patterns of CpG methylation, plays a key role in CAG repeat instability in human cells and in ..
  25. Kinney S, Moser M, Pascual M, Greally J, Foster B, Karpf A. Opposing roles of Dnmt1 in early- and late-stage murine prostate cancer. Mol Cell Biol. 2010;30:4159-74 pubmed publisher
    ..Here, we utilized Dnmt1 hypomorphic alleles to examine the role of Dnmt1 in normal prostate development and in prostate cancer in TRAMP...
  26. Weaver J, Sarkisian G, Krapp C, Mager J, Mann M, Bartolomei M. Domain-specific response of imprinted genes to reduced DNMT1. Mol Cell Biol. 2010;30:3916-28 pubmed publisher
    ..This methylation is critical for imprinting; a reduction in the DNA methyltransferase DNMT1 causes a widespread loss of imprinting...
  27. Hermann A, Goyal R, Jeltsch A. The Dnmt1 DNA-(cytosine-C5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites. J Biol Chem. 2004;279:48350-9 pubmed
    In the cell, Dnmt1 is the major enzyme in maintenance of the pattern of DNA methylation after DNA replication...
  28. Gaudet F, Talbot D, Leonhardt H, Jaenisch R. A short DNA methyltransferase isoform restores methylation in vivo. J Biol Chem. 1998;273:32725-9 pubmed
    ..While the longer MTase is associated with maintenance methyltransferase activity in replicating cells, little is known about the shorter ..
  29. Chung Y, Ratnam S, Chaillet J, Latham K. Abnormal regulation of DNA methyltransferase expression in cloned mouse embryos. Biol Reprod. 2003;69:146-53 pubmed
    ..Additionally, the maternal oocyte-derived Dnmt1o isoform undergoes little or none of its expected translocation to embryonic nuclei at the eight-cell stage...
  30. HUTNICK L, GOLSHANI P, Namihira M, Xue Z, Matynia A, Yang X, et al. DNA hypomethylation restricted to the murine forebrain induces cortical degeneration and impairs postnatal neuronal maturation. Hum Mol Genet. 2009;18:2875-88 pubmed publisher
    ..To understand the role of DNA methylation in central nervous system (CNS) neurons, we generated conditional Dnmt1 mutant mice that possess approximately 90% hypomethylated cortical and hippocampal cells in the dorsal forebrain ..
  31. Lin M, Lee T, Hsu D, Shen C. One-codon alternative splicing of the CpG MTase Dnmt1 transcript in mouse somatic cells. FEBS Lett. 2000;469:101-4 pubmed
    The genomic methylation patterns in the mammalian somatic cells are presumably maintained by a single enzyme, dnmt1. In mouse, this DNA (cytosine-5)-methyltransferase, or CpG MTase, is encoded by the Dnmt1 gene...
  32. Kimura H, Nakamura T, Ogawa T, Tanaka S, Shiota K. Transcription of mouse DNA methyltransferase 1 (Dnmt1) is regulated by both E2F-Rb-HDAC-dependent and -independent pathways. Nucleic Acids Res. 2003;31:3101-13 pubmed
    Abnormal expression of Dnmt1 in vivo induces cellular alterations such as transformation, and an increase in Dnmt1 mRNA plays a causal role in c-fos-, ras- and SV40 large T antigen-induced transformation of fibroblasts in vitro...
  33. Howell C, Bestor T, Ding F, Latham K, Mertineit C, Trasler J, et al. Genomic imprinting disrupted by a maternal effect mutation in the Dnmt1 gene. Cell. 2001;104:829-38 pubmed
    ..Mouse oocytes and preimplantation embryos lack Dnmt1 but express a variant of this protein called Dnmt1o. We eliminated Dnmt1o by deletion of the oocyte-specific promoter and first exon from the Dnmt1 locus...
  34. Mertineit C, Yoder J, Taketo T, Laird D, Trasler J, Bestor T. Sex-specific exons control DNA methyltransferase in mammalian germ cells. Development. 1998;125:889-97 pubmed
    ..The mRNA for Dnmt1, the predominant maintenance and de novo DNA (cytosine-5)-methyl transferase in mammals, is present at high levels ..
  35. Sado T, Fenner M, Tan S, Tam P, Shioda T, Li E. X inactivation in the mouse embryo deficient for Dnmt1: distinct effect of hypomethylation on imprinted and random X inactivation. Dev Biol. 2000;225:294-303 pubmed
    ..Using DNA methyltransferase 1 (Dnmt1)-deficient mouse embryos carrying X-linked lacZ transgenes, we studied the effects of genomic demethylation on X ..
  36. Qin W, Leonhardt H, Spada F. Usp7 and Uhrf1 control ubiquitination and stability of the maintenance DNA methyltransferase Dnmt1. J Cell Biochem. 2011;112:439-44 pubmed publisher
    In mammals Dnmt1 is the DNA methyltransferase chiefly responsible for maintaining genomic methylation patterns through DNA replication cycles, but how its maintenance activity is controlled is still not well understood...
  37. Ko Y, Nishino K, Hattori N, Arai Y, Tanaka S, Shiota K. Stage-by-stage change in DNA methylation status of Dnmt1 locus during mouse early development. J Biol Chem. 2005;280:9627-34 pubmed
    ..Three isoforms of Dnmt1 (DNA methyltransferase 1) transcripts, Dnmt1s, Dnmt1o, and Dnmt1p, are produced by alternative usage of multiple first exons. Dnmt1s is expressed in somatic cells...
  38. Borowczyk E, Mohan K, D Aiuto L, Cirio M, Chaillet J. Identification of a region of the DNMT1 methyltransferase that regulates the maintenance of genomic imprints. Proc Natl Acad Sci U S A. 2009;106:20806-11 pubmed publisher
    ..Because the DNMT1 cytosine methyltransferase governs maintenance methylation in mammals, rearrangements of non-DMD, but not DMD ..
  39. Gonzalo S, Jaco I, Fraga M, Chen T, Li E, Esteller M, et al. DNA methyltransferases control telomere length and telomere recombination in mammalian cells. Nat Cell Biol. 2006;8:416-24 pubmed
    ..Mouse embryonic stem (ES) cells genetically deficient for DNMT1, or both DNMT3a and DNMT3b have dramatically elongated telomeres compared with wild-type controls...
  40. Karimi M, Goyal P, Maksakova I, Bilenky M, Leung D, Tang J, et al. DNA methylation and SETDB1/H3K9me3 regulate predominantly distinct sets of genes, retroelements, and chimeric transcripts in mESCs. Cell Stem Cell. 2011;8:676-87 pubmed publisher
    ..H3K9 methyltransferase Setdb1 are distinct from those derepressed in mESC deficient in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b, with the exception of a small number of primarily germline-specific genes...
  41. Himes K, Koppes E, Chaillet J. Generalized disruption of inherited genomic imprints leads to wide-ranging placental defects and dysregulated fetal growth. Dev Biol. 2013;373:72-82 pubmed publisher
    ..we examined conceptuses developing in the absence of maternally derived DNA (cytosine-5-)-methyltransferase 1o (DNMT1o)...
  42. McGraw S, Oakes C, Martel J, Cirio M, de Zeeuw P, Mak W, et al. Loss of DNMT1o disrupts imprinted X chromosome inactivation and accentuates placental defects in females. PLoS Genet. 2013;9:e1003873 pubmed publisher
    ..methylation patterns during preimplantation development depends on stores of DNA cytosine methyltransferase-1o (DNMT1o) provided by the oocyte...
  43. Pradhan S, Talbot D, Sha M, Benner J, Hornstra L, Li E, et al. Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase. Nucleic Acids Res. 1997;25:4666-73 pubmed
    The original cDNA sequence reported for the murine DNA methyltransferase (MTase) was not full length...
  44. Tucker K, Beard C, Dausmann J, Jackson Grusby L, Laird P, Lei H, et al. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev. 1996;10:1008-20 pubmed
    Embryonic stem (ES) cells homozygous for a disruption of the DNA (cytosine-5)-methyltransferase gene (Dnmt) proliferate normally with their DNA highly demethylated but die upon differentiation...
  45. Lei H, Oh S, Okano M, Juttermann R, Goss K, Jaenisch R, et al. De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells. Development. 1996;122:3195-205 pubmed
    ..These results provide the first evidence that an independently encoded DNA methyltransferase is present in mammalian cells which is capable of de novo methylating cellular and viral DNA in vivo. ..
  46. Gaudet F, Hodgson J, Eden A, Jackson Grusby L, Dausman J, Gray J, et al. Induction of tumors in mice by genomic hypomethylation. Science. 2003;300:489-92 pubmed
    ..To explore this phenomenon, we generated mice carrying a hypomorphic DNA methyltransferase 1 (Dnmt1) allele, which reduces Dnmt1 expression to 10% of wild-type levels and results in substantial genome-wide ..
  47. Raiche J, Rodriguez Juarez R, Pogribny I, Kovalchuk O. Sex- and tissue-specific expression of maintenance and de novo DNA methyltransferases upon low dose X-irradiation in mice. Biochem Biophys Res Commun. 2004;325:39-47 pubmed
    ..In the current study, we examined the radiation-induced changes in expression of maintenance DNMT1, and de novo methyltransferases DNMT3a and DNMT3b in spleen and liver of irradiated animals...
  48. Aguirre Arteta A, Grunewald I, Cardoso M, Leonhardt H. Expression of an alternative Dnmt1 isoform during muscle differentiation. Cell Growth Differ. 2000;11:551-9 pubmed
    ..We identified an alternative Dnmt1 transcript in skeletal muscle by Northern blot analysis and cloned the corresponding cDNA by rapid amplification ..
  49. Glickman J, Pavlovich J, Reich N. Peptide mapping of the murine DNA methyltransferase reveals a major phosphorylation site and the start of translation. J Biol Chem. 1997;272:17851-7 pubmed
    ..Our peptide mapping results provide a more detailed structural characterization of the DNA methyltransferase that will facilitate future structure/function studies. ..
  50. Song J, Rechkoblit O, Bestor T, Patel D. Structure of DNMT1-DNA complex reveals a role for autoinhibition in maintenance DNA methylation. Science. 2011;331:1036-40 pubmed publisher
    Maintenance of genomic methylation patterns is mediated primarily by DNA methyltransferase-1 (DNMT1)...
  51. Schermelleh L, Haemmer A, Spada F, Rösing N, Meilinger D, Rothbauer U, et al. Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res. 2007;35:4301-12 pubmed
    ..of genomic methylation patterns was proposed to depend largely on the binding of DNA methyltransferase 1 (Dnmt1) to PCNA, a core component of the replication machinery...
  52. Rugo R, Mutamba J, Mohan K, Yee T, Chaillet J, Greenberger J, et al. Methyltransferases mediate cell memory of a genotoxic insult. Oncogene. 2011;30:751-6 pubmed publisher
    ..basis for the memory of an insult is completely dependent on two of the major DNA cytosine methyltransferases, Dnmt1 and Dnmt3a...
  53. Jackson M, Krassowska A, Gilbert N, Chevassut T, Forrester L, Ansell J, et al. Severe global DNA hypomethylation blocks differentiation and induces histone hyperacetylation in embryonic stem cells. Mol Cell Biol. 2004;24:8862-71 pubmed
    It has been reported that DNA methyltransferase 1-deficient (Dnmt1-/-) embryonic stem (ES) cells are hypomethylated (20% CpG methylation) and die through apoptosis when induced to differentiate...
  54. Guo G, Wang W, Bradley A. Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells. Nature. 2004;429:891-5 pubmed
    ..We demonstrate the recovery of cells with homozygous mutations in known and novel MMR genes. We identified Dnmt1(ref...
  55. Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo T, et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 2007;450:908-12 pubmed
    DNA methyltransferase (cytosine-5) 1 (Dnmt1) is the principal enzyme responsible for maintenance of CpG methylation and is essential for the regulation of gene expression, silencing of parasitic DNA elements, genomic imprinting and ..
  56. Cirio M, Ratnam S, Ding F, Reinhart B, Navara C, Chaillet J. Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints. BMC Dev Biol. 2008;8:9 pubmed publisher
    ..The related oocyte-derived Dnmt1o protein is also present in nuclei of 8-cell embryos, along with embryo-synthesized Dnmt1s...
  57. Doherty A, Bartolomei M, Schultz R. Regulation of stage-specific nuclear translocation of Dnmt1o during preimplantation mouse development. Dev Biol. 2002;242:255-66 pubmed
    ..Although the oocyte-specific form of Dnmt1 (Dnmt1o) possesses a functional nuclear localization signal, it is predominantly localized in the cytoplasm of the oocyte ..
  58. Jackson Grusby L, Beard C, Possemato R, Tudor M, Fambrough D, Csankovszki G, et al. Loss of genomic methylation causes p53-dependent apoptosis and epigenetic deregulation. Nat Genet. 2001;27:31-9 pubmed
    ..DNA methylation, we have generated mice with a conditional allele for the maintenance DNA methyltransferase gene Dnmt1. Cre-mediated deletion of Dnmt1 causes demethylation of cultured fibroblasts and a uniform p53-dependent cell ..
  59. Yoder J, Yen R, Vertino P, Bestor T, Baylin S. New 5' regions of the murine and human genes for DNA (cytosine-5)-methyltransferase. J Biol Chem. 1996;271:31092-7 pubmed
    ..Tanigawa, G., and Szyf, M. (1992) J. Biol. Chem. 267, 7368-7377) actually lies in an intron that is more than 5 kilobases downstream of the transcription start sites. ..
  60. Fuks F, Burgers W, Brehm A, Hughes Davies L, Kouzarides T. DNA methyltransferase Dnmt1 associates with histone deacetylase activity. Nat Genet. 2000;24:88-91 pubmed
    The DNA methyltransferase Dnmt1 is responsible for cytosine methylation in mammals and has a role in gene silencing...
  61. Bestor T. Activation of mammalian DNA methyltransferase by cleavage of a Zn binding regulatory domain. EMBO J. 1992;11:2611-7 pubmed
    ..Stimulation of the de novo activity of DNA methyltransferase by proteolytic cleavage in vivo may contribute to the process of ectopic methylation observed in the DNA of aging animals, tumors and in lines of cultured cells. ..
  62. Ding F, Chaillet J. In vivo stabilization of the Dnmt1 (cytosine-5)- methyltransferase protein. Proc Natl Acad Sci U S A. 2002;99:14861-6 pubmed
    The Dnmt1o form of the Dnmt1 (cytosine-5)-methyltransferase enzyme is synthesized and stored in the cytoplasm of the oocyte and is used after fertilization to maintain methylation patterns on imprinted genes...
  63. Sajedi E, Gaston Massuet C, Andoniadou C, Signore M, Hurd P, Dattani M, et al. DNMT1 interacts with the developmental transcriptional repressor HESX1. Biochim Biophys Acta. 2008;1783:131-43 pubmed
    ..molecular function of HESX1, we have performed a yeast two-hybrid screen and identified DNA methyltransferase 1 (DNMT1) as a HESX1 binding protein...
  64. Ghoshal K, Datta J, Majumder S, Bai S, Kutay H, Motiwala T, et al. 5-Aza-deoxycytidine induces selective degradation of DNA methyltransferase 1 by a proteasomal pathway that requires the KEN box, bromo-adjacent homology domain, and nuclear localization signal. Mol Cell Biol. 2005;25:4727-41 pubmed
    ..Here we report that among three functional DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), the maintenance methyltransferase, DNMT1, was rapidly degraded by the proteasomal pathway ..
  65. Eads C, Nickel A, Laird P. Complete genetic suppression of polyp formation and reduction of CpG-island hypermethylation in Apc(Min/+) Dnmt1-hypomorphic Mice. Cancer Res. 2002;62:1296-9 pubmed
    ..and tumorigenesis by genetically modulating expression levels of the predominant DNA methyltransferase, Dnmt1, in Apc(Min/+) mice...
  66. HUTNICK L, Huang X, Loo T, Ma Z, Fan G. Repression of retrotransposal elements in mouse embryonic stem cells is primarily mediated by a DNA methylation-independent mechanism. J Biol Chem. 2010;285:21082-91 pubmed publisher
    ..Surprisingly, in demethylated ESC cultures carrying mutations of DNA methyltransferase I (Dnmt1), no IAP transcripts and proteins are detectable in undifferentiated Oct4(+) ESCs. In contrast, approximately 3...
  67. Rountree M, Bachman K, Baylin S. DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci. Nat Genet. 2000;25:269-77 pubmed
    ..We show here that the chief enzyme that maintains mammalian DNA methylation, DNMT1, can also establish a repressive transcription complex...
  68. Endres M, Fan G, Meisel A, Dirnagl U, Jaenisch R. Effects of cerebral ischemia in mice lacking DNA methyltransferase 1 in post-mitotic neurons. Neuroreport. 2001;12:3763-6 pubmed
    DNA methylation is important for controlling gene expression and is catalyzed by DNA methyltransferase (Dnmt1) an enzyme abundant in brain...
  69. Wang K, James Shen C. DNA methyltransferase Dnmt1 and mismatch repair. Oncogene. 2004;23:7898-902 pubmed
    ..the relationship between DNA mismatch repair and deficiency of DNA methylation in a mouse embryonic cell line, Dnmt1-/- ES, with homologous deletion of the gene coding for the maintenance DNA methyltransferase Dnmt1...
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    We describe a transcriptional mechanism regulating the expression of Dnmt1 by nuclear receptors...
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    ..The implications of these findings for an understanding of the mechanisms involved in the establishment and maintenance of methylation patterns are discussed. ..
  72. Trinh B, Long T, Nickel A, Shibata D, Laird P. DNA methyltransferase deficiency modifies cancer susceptibility in mice lacking DNA mismatch repair. Mol Cell Biol. 2002;22:2906-17 pubmed
    We have introduced DNA methyltransferase 1 (Dnmt1) mutations into a mouse strain deficient for the Mlh1 protein to study the interaction between DNA mismatch repair deficiency and DNA methylation...
  73. Torrisani J, Unterberger A, Tendulkar S, Shikimi K, Szyf M. AUF1 cell cycle variations define genomic DNA methylation by regulation of DNMT1 mRNA stability. Mol Cell Biol. 2007;27:395-410 pubmed
    DNA methylation is a major determinant of epigenetic inheritance. DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division, and deregulated expression of DNMT1 leads ..
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    ..Our results demonstrate that a normal level of DNA methylation is required for controlling differential expression of the paternal and maternal alleles of imprinted genes. ..
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    ..Using Dnmt1 hypomorphic alleles to reduce genomic methylation, we observed elevated incidence of microadenomas that were ..
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    ..Previous reports suggested that DNA methyltransferase(s) other than Dnmt1 is involved in the maintenance of the imprints during cleavage...
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    ..In this study, we characterized the GC-rich promoter of the gene for the DNA methyltransferase (Dnmt1) that is responsible for methylation of cytosine residues in mammals and plays a role in gene silencing...