Gene Symbol: Dnajb1
Description: DnaJ heat shock protein family (Hsp40) member B1
Alias: 0610007I11Rik, DjB1, HSPF1, Hdj1, Hsp40, dnaJ homolog subfamily B member 1, DnaJ (Hsp40) homolog, subfamily B, member 1, heat shock 40 kDa protein 1
Species: mouse
Products:     Dnajb1

Top Publications

  1. Jana N, Tanaka M, Wang G, Nukina N. Polyglutamine length-dependent interaction of Hsp40 and Hsp70 family chaperones with truncated N-terminal huntingtin: their role in suppression of aggregation and cellular toxicity. Hum Mol Genet. 2000;9:2009-18 pubmed
    ..Here we demonstrate that newly synthesized polyglutamine-expanded truncated huntingtin interacts with members of Hsp40 and Hsp70 families of chaperones in a polyglutamine length-dependent manner...
  2. Gibbs S, Barren B, Beck K, Proft J, Zhao X, Noskova T, et al. Hsp40 couples with the CSPalpha chaperone complex upon induction of the heat shock response. PLoS ONE. 2009;4:e4595 pubmed publisher
    ..we demonstrate that in response to heat shock or treatment with the Hsp90 inhibitor geldanamycin, the J protein Hsp40 becomes a major component of the CSPalpha complex...
  3. Uchiyama Y, Takeda N, Mori M, Terada K. Heat shock protein 40/DjB1 is required for thermotolerance in early phase. J Biochem. 2006;140:805-12 pubmed
    DjB1 (Hsp40/DnajB1/Hdj1) is a member of the Hsp40/DnaJ family that functions as a co-chaperone of mammalian Hsp70s. DjB1 recognizes substrate proteins and facilitates the ATPase activity of Hsp70. We generated DjB1 deficient mice...
  4. Grillo S, Duggett N, Ennaceur A, Chazot P. Non-invasive infra-red therapy (1072 nm) reduces ?-amyloid protein levels in the brain of an Alzheimer's disease mouse model, TASTPM. J Photochem Photobiol B. 2013;123:13-22 pubmed publisher
    ..During the first 12 months of age, a critical period of AD progression, reduced HSP40 and HSP105 were observed...
  5. Carnini A, Scott L, Ahrendt E, Proft J, Winkfein R, Kim S, et al. Cell line specific modulation of extracellular a?42 by Hsp40. PLoS ONE. 2012;7:e37755 pubmed publisher
    ..Moreover, transient transfection of the stress induced Hsp40, decreased CAD levels of A?(42)...
  6. Zhu Y, Zhu J, Wan X, Zhu Y, Zhang T. Gene expression of sHsps, Hsp40 and Hsp60 families in normal and abnormal embryonic development of mouse forelimbs. Toxicol Lett. 2010;193:242-51 pubmed publisher
    ..The present study detected expression of sHsps, Hsp40 and Hsp60 in normal and abnormal development of embryonic forelimbs in all-trans retinoic acid (atRA)-induced ..
  7. Rodriguez K, Osmulski P, Pierce A, Weintraub S, Gaczynska M, Buffenstein R. A cytosolic protein factor from the naked mole-rat activates proteasomes of other species and protects these from inhibition. Biochim Biophys Acta. 2014;1842:2060-72 pubmed publisher
    ..Although Heat shock protein 72 kDa (HSP72) and Heat shock protein 40 kDa (Homolog of bacterial DNAJ1) (HSP40(Hdj1)) are among the constituents of this factor, the observed phenomenon, such as increasing peptidase activity ..
  8. Ko S, Kim J, Na D, Park S, Park S, Hyun J, et al. A small molecule inhibitor of ATPase activity of HSP70 induces apoptosis and has antitumor activities. Chem Biol. 2015;22:391-403 pubmed publisher
    ..Affinity chromatography provides evidence that Az binds HSP70 but not other types of heat shock proteins including HSP40, HSP60, and HSP90...
  9. Pemberton S, Madiona K, Pieri L, Kabani M, Bousset L, Melki R. Hsc70 protein interaction with soluble and fibrillar alpha-synuclein. J Biol Chem. 2011;286:34690-9 pubmed publisher
    ..between Hsc70 and ?-Syn, the consequences of this interaction, and the role of nucleotides and co-chaperones Hdj1 and Hdj2 as modulators...

More Information


  1. Fujimoto M, Izu H, Seki K, Fukuda K, Nishida T, Yamada S, et al. HSF4 is required for normal cell growth and differentiation during mouse lens development. EMBO J. 2004;23:4297-306 pubmed
  2. Hata M, Ohtsuka K. Cloning and expression of murine Hsp40 gene: differences in initiation sites between heat-induced and constitutive transcripts. DNA Seq. 2000;11:213-23 pubmed
    We have isolated cDNA and genomic clones of murine Hsp40 (mmDjB1). The murine Hsp40 gene was expressed at a high level in testis and was induced by heat shock...
  3. Doiguchi M, Kaneko T, Urasoko A, Nishitani H, Iida H. Identification of a heat-shock protein Hsp40, DjB1, as an acrosome- and a tail-associated component in rodent spermatozoa. Mol Reprod Dev. 2007;74:223-32 pubmed
    ..To characterize DjB1 that is encoded by Dnajb1 gene, we carried out immunoblot analyses, in situ hybridization, and immunohistochemistry...
  4. Khan S, Vasanth S, Kabir F, Gottsch J, Khan A, Chaerkady R, et al. FOXE3 contributes to Peters anomaly through transcriptional regulation of an autophagy-associated protein termed DNAJB1. Nat Commun. 2016;7:10953 pubmed publisher
    ..We found that DNAJB1, an autophagy-associated protein, was the only candidate exhibiting differential expression in both screens...
  5. Kampinga H, Hageman J, Vos M, Kubota H, Tanguay R, Bruford E, et al. Guidelines for the nomenclature of the human heat shock proteins. Cell Stress Chaperones. 2009;14:105-11 pubmed publisher
    ..new guidelines for the nomenclature of the human HSP families, HSPH (HSP110), HSPC (HSP90), HSPA (HSP70), DNAJ (HSP40), and HSPB (small HSP) as well as for the human chaperonin families HSPD/E (HSP60/HSP10) and CCT (TRiC)...
  6. Chen M, Roti J, Laszlo A. Hsc40, a new member of the hsp40 family, exhibits similar expression profile to that of hsc70 in mammalian cells. Gene. 1999;238:333-41 pubmed
    Here, we report the identification and characterization of a new hsp40 family member, heat shock cognate 40 (hsc40), which may be a specific functional partner for hsc70. The hsc40 gene consists of five exons and four introns...
  7. Nagaoka U, Kim K, Jana N, Doi H, Maruyama M, Mitsui K, et al. Increased expression of p62 in expanded polyglutamine-expressing cells and its association with polyglutamine inclusions. J Neurochem. 2004;91:57-68 pubmed
    ..These results suggest that p62 may play important roles as a responsive protein to a polyglutamine-induced stress rather than as a cross-linker between ubiquitylated proteins. ..
  8. Tanaka S, Kitagawa K, Ohtsuki T, Yagita Y, Takasawa K, Hori M, et al. Synergistic induction of HSP40 and HSC70 in the mouse hippocampal neurons after cerebral ischemia and ischemic tolerance in gerbil hippocampus. J Neurosci Res. 2002;67:37-47 pubmed
    ..One of the ischemia-responsive clones was found to encode mouse hsp40. HSP40 has a critical regulatory function in the HSC70 ATPase activity...
  9. Engelholm L, Riaz A, Serra D, Dagnæs Hansen F, Johansen J, Santoni Rugiu E, et al. CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates That the Dnajb1-Prkaca Gene Fusion Is Sufficient to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma. Gastroenterology. 2017;153:1662-1673.e10 pubmed publisher
    ..400 Kb deletion on chromosome 19 that fuses part of the DnaJ heat shock protein family (Hsp40) member B1 gene (DNAJB1) to the protein kinase cAMP-activated catalytic subunit alpha gene (PRKACA) has been repeatedly identified in ..
  10. Islam A, Deuster P, Devaney J, Ghimbovschi S, Chen Y. An exploration of heat tolerance in mice utilizing mRNA and microRNA expression analysis. PLoS ONE. 2013;8:e72258 pubmed publisher
    ..05). Among them, Hspa1a, Dnajb1 and Hspb7 were differentially expressed by more than two-fold under these conditions...
  11. Park S, Hong J, Arslan F, Kanneganti V, Patel B, Tietsort A, et al. Overexpression of the essential Sis1 chaperone reduces TDP-43 effects on toxicity and proteolysis. PLoS Genet. 2017;13:e1006805 pubmed publisher
    ..Furthermore, overexpression of the mammalian Sis1 homologue, DNAJB1, relieves TDP-43 mediated toxicity in primary rodent cortical neurons, suggesting that Sis1 and its homologues may ..
  12. Couturier M, Buccellato M, Costanzo S, Bourhis J, Shu Y, Nicaise M, et al. High affinity binding between Hsp70 and the C-terminal domain of the measles virus nucleoprotein requires an Hsp40 co-chaperone. J Mol Recognit. 2010;23:301-15 pubmed publisher
    ..The current work showed that high affinity hsp70 binding to N(TAIL) requires an hsp40 co-chaperone that interacts primarily with the hsp70 nucleotide binding domain (NBD) and displays no significant ..
  13. Wang G, Zhang J, Moskophidis D, Mivechi N. Targeted disruption of the heat shock transcription factor (hsf)-2 gene results in increased embryonic lethality, neuronal defects, and reduced spermatogenesis. Genesis. 2003;36:48-61 pubmed
    ..These findings suggest that hsf2 has a major function in controlling expression of genes important for embryonic development and maintenance of sperm production. ..
  14. Gupta S, Wang L, Anderl J, Slifker M, Kirk C, Kruger W. Correction of cystathionine ?-synthase deficiency in mice by treatment with proteasome inhibitors. Hum Mutat. 2013;34:1085-93 pubmed publisher
    ..We show that treatment of these mice with two different proteasome inhibitors can induce liver Hsp70, Hsp40, and Hsp27, increase levels of active CBS, and lower plasma homocysteine levels to within the normal range...
  15. Yan Z, Wei H, Ren C, Yuan S, Fu H, Lv Y, et al. Gene expression of Hsps in normal and abnormal embryonic development of mouse hindlimbs. Hum Exp Toxicol. 2015;34:563-74 pubmed publisher
    ..Our previous studies found that Hsp25, HspB2, HspB3, HspB7, Hsp20, HspB9, HspB10, and Hsp40 may be related to all-trans retinoic acid (atRA)-induced phocomelic and other abnormalities, while HspA12B, HspA14,..
  16. Bagchi M, Besser D, Reddy T, Skoff R, Maisel H. Effect of thermal stress on early and late passaged mouse lens epithelial cells. J Cell Biochem. 2007;102:1036-42 pubmed
    ..The data presented here suggests a relationship between the presence of functional HSF-1 and sustained proliferative activities of the late passaged alphaTN-4 cell. ..
  17. Yamagishi N, Ishihara K, Saito Y, Hatayama T. Hsp105 but not Hsp70 family proteins suppress the aggregation of heat-denatured protein in the presence of ADP. FEBS Lett. 2003;555:390-6 pubmed
    ..In contrast, Hsc70/Hsp40 suppressed the aggregation of heat-denatured protein in the presence of ATP rather than ADP...
  18. Popiel H, Takeuchi T, Fujita H, Yamamoto K, Ito C, Yamane H, et al. Hsp40 gene therapy exerts therapeutic effects on polyglutamine disease mice via a non-cell autonomous mechanism. PLoS ONE. 2012;7:e51069 pubmed publisher
    ..We found that injection of adeno-associated virus type 5 (AAV5) expressing QBP1 or Hsp40 into the striatum both dramatically suppresses inclusion body formation in the HD mouse R6/2...
  19. Mishra A, Dikshit P, Purkayastha S, Sharma J, Nukina N, Jana N. E6-AP promotes misfolded polyglutamine proteins for proteasomal degradation and suppresses polyglutamine protein aggregation and toxicity. J Biol Chem. 2008;283:7648-56 pubmed publisher
    ..These findings suggest that E6-AP is a critical mediator of the neuronal response to misfolded polyglutamine proteins and represents a potential therapeutic target in the polyglutamine diseases...
  20. Huang L, Min J, Masters S, Mivechi N, Moskophidis D. Insights into function and regulation of small heat shock protein 25 (HSPB1) in a mouse model with targeted gene disruption. Genesis. 2007;45:487-501 pubmed