Dffa

Summary

Gene Symbol: Dffa
Description: DNA fragmentation factor, alpha subunit
Alias: A330085O09Rik, DFF35, Dff45, ICAD, ICAD-L, ICAD-S, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, inhibitor of CAD
Species: mouse
Products:     Dffa

Top Publications

  1. Zhang J, Liu X, Scherer D, van Kaer L, Wang X, Xu M. Resistance to DNA fragmentation and chromatin condensation in mice lacking the DNA fragmentation factor 45. Proc Natl Acad Sci U S A. 1998;95:12480-5 pubmed
    The DNA fragmentation factor 45 (DFF45) is a subunit of a heterodimeric nuclease complex critical for the induction of DNA fragmentation in vitro...
  2. Slane McQuade J, Vorhees C, Xu M, Zhang J. DNA fragmentation factor 45 knockout mice exhibit longer memory retention in the novel object recognition task compared to wild-type mice. Physiol Behav. 2002;76:315-20 pubmed
    ..To study the role of DNA fragmentation factor 45 (DFF45/ICAD) in CNS function, we previously generated DFF45 knockout mice...
  3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature. 1998;391:96-9 pubmed
    ..Article, we have identified and molecularly cloned a caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD). Here we show that caspase 3 cleaves ICAD and inactivates its CAD-inhibitory effect...
  4. Boulares A, Zoltoski A, Sherif Z, Yakovlev A, Smulson M. Roles of DNA fragmentation factor and poly(ADP-ribose) polymerase-1 in sensitization of fibroblasts to tumor necrosis factor-induced apoptosis. Biochem Biophys Res Commun. 2002;290:796-801 pubmed
    DNA fragmentation factor (DFF) comprises DFF45 and DFF40 subunits, the former of which acts as an inhibitor of the latter (the catalytic subunit) and whose cleavage by caspase-3 results in DFF activation...
  5. Zhang J, Lee H, Lou D, Bovin G, Xu M. Lack of obvious 50 kilobase pair DNA fragments in DNA fragmentation factor 45-deficient thymocytes upon activation of apoptosis. Biochem Biophys Res Commun. 2000;274:225-9 pubmed
    The DNA fragmentation factor 45 (DFF45/ICAD) is a key subunit of a heterodimeric DNase complex critical for the induction of DNA fragmentation during apoptosis in vivo...
  6. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature. 1998;391:43-50 pubmed
    ..A caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD) have now been identified in the cytoplasmic fraction of mouse lymphoma cells...
  7. Yan B, Wang H, Peng Y, Hu Y, Wang H, Zhang X, et al. A unique role of the DNA fragmentation factor in maintaining genomic stability. Proc Natl Acad Sci U S A. 2006;103:1504-9 pubmed
  8. Zhang J, Wang X, Bove K, Xu M. DNA fragmentation factor 45-deficient cells are more resistant to apoptosis and exhibit different dying morphology than wild-type control cells. J Biol Chem. 1999;274:37450-4 pubmed
    The DNA fragmentation factor 45 (DFF45) is a subunit of a heterodimeric DNase complex critical for the induction of DNA fragmentation in vitro...
  9. Errami Y, Brim H, Oumouna Benachour K, Oumouna M, Naura A, Kim H, et al. ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability. PLoS ONE. 2013;8:e57871 pubmed publisher
    ..showed that DNA fragmentation factor, which comprises a caspase-3-activated DNase (CAD) and its inhibitor (ICAD), may influence the rate of cell death by generating PARP-1-activating DNA breaks...

More Information

Publications21

  1. Kutscher D, Pingoud A, Jeltsch A, Meiss G. Identification of ICAD-derived peptides capable of inhibiting caspase-activated DNase. FEBS J. 2012;279:2917-28 pubmed publisher
    ..fragmentation factor is a heterodimeric complex that consists of caspase-activated DNase (CAD) and its inhibitor (ICAD)...
  2. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 1998;17:2526-33 pubmed
    b>DFF45 is a subunit of the DNA fragmentation factor (DFF) that is cleaved by caspase-3 during apoptosis. However, the mechanism by which DFF45 regulates apoptotic cell death remains poorly understood...
  3. Zhang J, Lee H, Agarwala A, Wen Lou D, Xu M. Dna fragmentation factor 45 mutant mice exhibit resistance to kainic acid-induced neuronal cell death. Biochem Biophys Res Commun. 2001;285:1143-9 pubmed
    ..The DNA fragmentation factor 45 (DFF45 or ICAD) is a subunit of a heterodimeric DNase complex crucial for DNA fragmentation during apoptosis...
  4. Phillips R, Ernst R, Brunk B, Ivanova N, Mahan M, Deanehan J, et al. The genetic program of hematopoietic stem cells. Science. 2000;288:1635-40 pubmed
    ..The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell. ..
  5. Inohara N, Koseki T, Chen S, Benedict M, Nunez G. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J Biol Chem. 1999;274:270-4 pubmed
    ..DFF) is composed of two subunits, the 40-kDa caspase-3-activated nuclease (DFF40/CAD) and its 45-kDa inhibitor (DFF45/ICAD). During apoptosis, DFF-40/CAD is activated by caspase-3-mediated cleavage of DFF45/ICAD...
  6. Chen D, Stetler R, Cao G, Pei W, O Horo C, Yin X, et al. Characterization of the rat DNA fragmentation factor 35/Inhibitor of caspase-activated DNase (Short form). The endogenous inhibitor of caspase-dependent DNA fragmentation in neuronal apoptosis. J Biol Chem. 2000;275:38508-17 pubmed
    ..We have cloned the rat DNA fragmentation factor 35/inhibitor of caspase-activated DNase (short form) (DFF35/ICAD(S)) and found it to be the predominant form of ICAD present in rodent brain cells as well as in many other ..
  7. Slane J, Lee H, Vorhees C, Zhang J, Xu M. DNA fragmentation factor 45 deficient mice exhibit enhanced spatial learning and memory compared to wild-type control mice. Brain Res. 2000;867:70-9 pubmed
    ..The DNA fragmentation factor 45 (DFF45 or ICAD) is a subunit of a heterodimeric DNase complex that is crucial for DNA fragmentation and normal apoptosis...
  8. Korn C, Scholz S, Gimadutdinow O, Lurz R, Pingoud A, Meiss G. Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state. J Biol Chem. 2005;280:6005-15 pubmed
    DNA fragmentation factor (DFF) is a complex of the DNase DFF40 (CAD) and its chaperone/inhibitor DFF45 (ICAD-L) that can be activated during apoptosis to induce DNA fragmentation...
  9. Xiao F, Widlak P, Garrard W. Engineered apoptotic nucleases for chromatin research. Nucleic Acids Res. 2007;35:e93 pubmed
    ..Normally, in its inactive form, DFF is a heterodimer composed of a 45-kDa chaperone inhibitor subunit (DFF45 or ICAD), and a 40-kDa latent endonuclease subunit (DFF40 or CAD)...
  10. Otomo T, Sakahira H, Uegaki K, Nagata S, Yamazaki T. Structure of the heterodimeric complex between CAD domains of CAD and ICAD. Nat Struct Biol. 2000;7:658-62 pubmed
    ..complex between the CAD domain of caspase activated deoxyribonuclease (CAD) and the CAD domain of its inhibitor (ICAD), determined by nuclear magnetic resonance spectroscopy...
  11. Zhang J, Dong M, Li L, Fan Y, Pathre P, Dong J, et al. Endonuclease G is required for early embryogenesis and normal apoptosis in mice. Proc Natl Acad Sci U S A. 2003;100:15782-7 pubmed
    ..These findings indicate that EndoG is essential during early embryogenesis and plays a critical role in normal apoptosis and nuclear DNA fragmentation. ..
  12. Fukushima K, Kikuchi J, Koshiba S, Kigawa T, Kuroda Y, Yokoyama S. Solution structure of the DFF-C domain of DFF45/ICAD. A structural basis for the regulation of apoptotic DNA fragmentation. J Mol Biol. 2002;321:317-27 pubmed
    ..The structure of DFF-C also provides a rationale for the loss of the chaperone activity in DFF35, a short isoform of DFF45...