Gene Symbol: Cyp2b10
Description: cytochrome P450, family 2, subfamily b, polypeptide 10
Alias: Cyp2b, Cyp2b20, p16, cytochrome P450 2B10, CYPIIB10, CYPIIB20, P24, cytochrome P450 2B20, cytochrome P450 clone PF3/46, cytochrome P450, 2b10, phenobarbitol inducible, type b, cytochrome P450, 2b20, cytochrome P450, family 2, subfamily b, polypeptide 20, cytochrome P450-16-alpha, phenobarbitol inducible, type b, testosterone 16-alpha hydroxylase
Species: mouse
Products:     Cyp2b10

Top Publications

  1. Meyer S, Jirtle R. Old dance with a new partner: EGF receptor as the phenobarbital receptor mediating Cyp2B expression. Sci Signal. 2013;6:pe16 pubmed publisher
    The decades-long quest for the phenobarbital (PhB) receptor that mediates activation of Cyp2B would appear fulfilled with the discovery by Mutoh et al...
  2. Abbott B, Wood C, Watkins A, Tatum Gibbs K, Das K, Lau C. Effects of perfluorooctanoic acid (PFOA) on expression of peroxisome proliferator-activated receptors (PPAR) and nuclear receptor-regulated genes in fetal and postnatal CD-1 mouse tissues. Reprod Toxicol. 2012;33:491-505 pubmed publisher
    ..The metabolic disruption produced by PFOA may contribute to poor postnatal survival and persistent weight deficits of CD-1 mouse neonates. ..
  3. Rivera Rivera I, Kim J, Kemper B. Transcriptional analysis in vivo of the hepatic genes, Cyp2b9 and Cyp2b10, by intravenous administration of plasmid DNA in mice. Biochim Biophys Acta. 2003;1619:254-62 pubmed
    ..In this assay PB treatment increased transactivation by the Cyp2b10 PBRU about 100-fold, which is similar to the increase in the expression of the endogenous gene while the Cyp2b9 ..
  4. Atlas S, Zweier J, Nebert D. Genetic differences in phenytoin pharmacokinetics. In vivo clearance and in vitro metabolism among inbred strains of mice. Dev Pharmacol Ther. 1980;1:281-304 pubmed
  5. Kipling D, Ackford H, Taylor B, Cooke H. Mouse minor satellite DNA genetically maps to the centromere and is physically linked to the proximal telomere. Genomics. 1991;11:235-41 pubmed
    ..The apparent lack of any sizable amount of nonsatellite DNA between the minor satellite and the terminal repeat arrays indicates that many mouse chromosomes are truly telocentric. ..
  6. Park Y, Lee E, Lee Y, Park D, Jang H, Moore D. Opposing regulation of cytochrome P450 expression by CAR and PXR in hypothyroid mice. Toxicol Appl Pharmacol. 2012;263:131-7 pubmed publisher
    ..In contrast, expression of Cyp2b10 was induced in both WT and PXR-/- hypothyroid mice, and this induction was abolished in CAR-/- mice and in and ..
  7. Honkakoski P, Moore R, Gynther J, Negishi M. Characterization of phenobarbital-inducible mouse Cyp2b10 gene transcription in primary hepatocytes. J Biol Chem. 1996;271:9746-53 pubmed
    The mouse phenobarbital (PB)-inducible Cyp2b10 gene promoter has been isolated and sequenced, and control of its expression has been characterized...
  8. Koga T, Yao P, Goudarzi M, Murray I, Balandaram G, Gonzalez F, et al. Regulation of Cytochrome P450 2B10 (CYP2B10) Expression in Liver by Peroxisome Proliferator-activated Receptor-?/? Modulation of SP1 Promoter Occupancy. J Biol Chem. 2016;291:25255-25263 pubmed
    ..Chronic ethanol treatment causes increased hepatic CYP2B10 expression inPpar?/?+/+ mice but not in Ppar?/?-/- mice...
  9. Gachon F, Olela F, Schaad O, Descombes P, Schibler U. The circadian PAR-domain basic leucine zipper transcription factors DBP, TEF, and HLF modulate basal and inducible xenobiotic detoxification. Cell Metab. 2006;4:25-36 pubmed
    ..Indeed, PAR bZip triple knockout mice are hypersensitive to xenobiotic compounds, and the deficiency in detoxification may contribute to their early aging. ..

More Information


  1. Wang H, Donley K, Keeney D, Hoffman S. Organization and evolution of the Cyp2 gene cluster on mouse chromosome 7, and comparison with the syntenic human cluster. Environ Health Perspect. 2003;111:1835-42 pubmed
    ..The mouse cluster includes 22 loci from the same six CYP2 subfamilies--Cyp2a, Cyp2b, Cyp2f, Cyp2g, Cyp2s, Cyp2t--that are found in the human cluster...
  2. Marc N, Damon M, Fautrel A, Guillouzo A, Corcos L. Isolation of a cyp2b10-like cDNA and of a clone derived from a cyp2b10-like pseudogene. Biochem Biophys Res Commun. 1999;258:11-6 pubmed
    By screening Balb/c male mouse liver cDNA library with a rat CYP2B1 cDNA probe, we have isolated a 1795 bp cyp2b10-like clone, referred to as P16...
  3. Damon M, Fautrel A, Marc N, Guillouzo A, Corcos L. Isolation of a new mouse cDNA clone: hybrid form of cytochrome P450 2b10 and NADPH-cytochrome P450 oxidoreductase. Biochem Biophys Res Commun. 1996;226:900-5 pubmed
    ..consists of an open reading frame containing no stop codon of 1348 bp which share 98% similarity with the cytochrome P450 2b10 followed by 95 nucleotides sharing 88% identity with the mouse NADPH cytochrome P450 oxidoreductase...
  4. Burkhart B, Skow L, Negishi M. Two steroid 15 alpha-hydroxylase genes and a homologous gene family in mice. Gene. 1990;87:205-11 pubmed
    ..Analysis of a restriction fragment length polymorphism in recombinant inbred mice shows a close linkage of 15 alpha oh-1 and 15 alpha oh-2 with the Coh locus. ..
  5. Gonzalez F, Kasper C. Cloning of DNA complementary to rat liver NADPH-cytochrome c (P-450) oxidoreductase and cytochrome P-450b mRNAs. Evidence that phenobarbital augments transcription of specific genes. J Biol Chem. 1982;257:5962-8 pubmed
  6. Gnerre C, Schuster G, Roth A, Handschin C, Johansson L, Looser R, et al. LXR deficiency and cholesterol feeding affect the expression and phenobarbital-mediated induction of cytochromes P450 in mouse liver. J Lipid Res. 2005;46:1633-42 pubmed
    ..Our data expose differential regulatory patterns for Cyp2b10 and Cyp3a11 dependent on the expression of LXR isoforms and on challenge of cholesterol homeostasis by excess ..
  7. Jugert F, Agarwal R, Kuhn A, Bickers D, Merk H, Mukhtar H. Multiple cytochrome P450 isozymes in murine skin: induction of P450 1A, 2B, 2E, and 3A by dexamethasone. J Invest Dermatol. 1994;102:970-5 pubmed
  8. Bornheim L, Everhart E, Li J, Correia M. Induction and genetic regulation of mouse hepatic cytochrome P450 by cannabidiol. Biochem Pharmacol. 1994;48:161-71 pubmed
    ..The mechanism of CBD-mediated P450 induction remains to be elucidated but does not appear to be related to CBD-mediated P450 inactivation. ..
  9. Keeney D. The novel skin-specific cytochrome P450 Cyp2b19 maps to proximal chromosome 7 in the mouse, near a cluster of Cyp2 family genes. Genomics. 1998;53:417-9 pubmed
  10. Honkakoski P, Negishi M. Protein serine/threonine phosphatase inhibitors suppress phenobarbital-induced Cyp2b10 gene transcription in mouse primary hepatocytes. Biochem J. 1998;330 ( Pt 2):889-95 pubmed
    Using a primary hepatocyte culture in which the mouse Cyp2b10 gene transcription is activated by phenobarbital (PB)-type inducers, we examined the cellular signalling mechanisms associated with PB induction...
  11. Lachaud A, Auclair Vincent S, Massip L, Audet Walsh E, Lebel M, Anderson A. Werner's syndrome helicase participates in transcription of phenobarbital-inducible CYP2B genes in rat and mouse liver. Biochem Pharmacol. 2010;79:463-70 pubmed publisher
    ..The rat CYP2B2 gene and its closely related mouse homolog, Cyp2b10, are both strongly induced in liver by phenobarbital...
  12. Noshiro M, Lakso M, Kawajiri K, Negishi M. Rip locus: regulation of female-specific isozyme (I-P-450(16 alpha) of testosterone 16 alpha-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA. Biochemistry. 1988;27:6434-43 pubmed
    ..abstract truncated at 400 words) ..
  13. Chiang P, Stubbs L, Zhang L, Kurnit D. Isolation of murine SPT5 homologue: completion of the isolation and characterization of human and murine homologues of yeast chromatin structural protein complex SPT4, SPT5, and SPT6. Genomics. 1998;47:426-8 pubmed
  14. Bösl M, Seldin M, Nishimura S, Taketo M. Cloning, structural analysis and mapping of the mouse selenocysteine tRNA([Ser]Sec) gene (Trsp). Mol Gen Genet. 1995;248:247-52 pubmed
  15. Ashino T, Ohkubo Morita H, Yamamoto M, Yoshida T, Numazawa S. Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5. Redox Biol. 2014;2:284-8 pubmed publisher
    ..factor erythroid 2-related factor 2 (Nrf2)-deficient (Nrf2(-?-)) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression...
  16. Keeney D, Skinner C, Travers J, Capdevila J, Nanney L, King L, et al. Differentiating keratinocytes express a novel cytochrome P450 enzyme, CYP2B19, having arachidonate monooxygenase activity. J Biol Chem. 1998;273:32071-9 pubmed
  17. Handschin C, Meyer U. A conserved nuclear receptor consensus sequence (DR-4) mediates transcriptional activation of the chicken CYP2H1 gene by phenobarbital in a hepatoma cell line. J Biol Chem. 2000;275:13362-9 pubmed
    ..phenobarbital response elements in the flanking regions of three phenobarbital-inducible genes, rat CYP2B2, mouse Cyp2b10, and human CYP2B6...
  18. Miles J, Moss J, Meehan R, Wolf C. Close linkage of the cytochrome P450IIA gene subfamily (Cyp2a) to Cyp2b and Coh on mouse chromosome 7. Genomics. 1990;7:445-8 pubmed
    The cytochrome P450IIB gene subfamily (Cyp2b) has previously been mapped close to the Coh locus encoding a cytochrome P450 with coumarin 7-hydroxylase (COH) activity on mouse chromosome 7...
  19. Bornheim L, Correia M. Purification and characterization of a mouse liver cytochrome P-450 induced by cannabidiol. Mol Pharmacol. 1989;36:377-83 pubmed
    ..Thus, CBD exposure results in the induction of an isozyme that is refractory to CBD-mediated inactivation, thereby apparently altering the cytochrome P-450 isozymal composition of mouse hepatic microsomes. ..
  20. Ohno M, Kanayama T, Moore R, Ray M, Negishi M. The roles of co-chaperone CCRP/DNAJC7 in Cyp2b10 gene activation and steatosis development in mouse livers. PLoS ONE. 2014;9:e115663 pubmed publisher
    ..Despite this accumulation, phenobarbital-induced activation of the Cyp2b10 gene was significantly attenuated...
  21. Lengler J, Omann M, Düvier D, Holzmüller H, Gregor W, Salmons B, et al. Cytochrome P450 reductase dependent inhibition of cytochrome P450 2B1 activity: Implications for gene directed enzyme prodrug therapy. Biochem Pharmacol. 2006;72:893-901 pubmed
  22. Damiri B, Holle E, Yu X, Baldwin W. Lentiviral-mediated RNAi knockdown yields a novel mouse model for studying Cyp2b function. Toxicol Sci. 2012;125:368-81 pubmed publisher
    ..activator, 1,4-Bis[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP), was used to determine if shRNA-mediated Cyp2b10 repression could be outcompeted by Cyp2b10 induction...
  23. Katoh M, Katoh M. Identification and characterization of human FNBP3 gene in silico. Int J Mol Med. 2003;12:651-6 pubmed
    ..On the other hand, FNBP3 with nuclear localization signals and two tyrosine phosphorylation sites might transduce extracellular signals to the nucleus. This is the first report on comprehensive characterization of human FNBP3 gene. ..
  24. Honkakoski P, Lang M. Mouse liver phenobarbital-inducible P450 system: purification, characterization, and differential inducibility of four cytochrome P450 isozymes from D2 mouse. Arch Biochem Biophys. 1989;273:42-57 pubmed
    ..This mouse PB-inducible P450 model may be valuable in further studies on the induction mechanisms of PB and TCPOBOP. ..
  25. Saunders A, Seldin M. The syntenic relationship of proximal mouse chromosome 7 and the myotonic dystrophy gene region on human chromosome 19q. Genomics. 1990;6:324-32 pubmed
    ..Their order from the centromere was Prkcg, [Apoe, Atpa-2, Ckmm, D19S19h, Ercc-2], Cyp2b, Mag, Lhb. Two other murine loci, D7Rp2 and Ngfg, were also positioned within this interval...
  26. Nemoto N, Sakurai J. Glucocorticoid and sex hormones as activating or modulating factors for expression of Cyp2b-9 and Cyp2b-10 in the mouse liver and hepatocytes. Arch Biochem Biophys. 1995;319:286-92 pubmed
    The effects of male and female hormones as well as glucocorticoid upon the expression of Cyp2b-9 and Cyp2b-10 were investigated in C57BL/6 mouse liver and hepatocytes in primary culture...
  27. Cheng X, Klaassen C. Perfluorocarboxylic acids induce cytochrome P450 enzymes in mouse liver through activation of PPAR-alpha and CAR transcription factors. Toxicol Sci. 2008;106:29-36 pubmed publisher
    ..50 mg/kg) of perfluorooctanoic acid (PFOA) and perfluorodecanoic acid (PFDA) increased mRNA expression of Cyp2B10 (20-fold), 3A11 (two-fold), and 4A14 (32-fold), but not Cyp1A1/2 in mouse livers...
  28. Lundgren M, Darnerud P, Molin Y, Lilienthal H, Blomberg J, Ilback N. Viral infection and PBDE exposure interact on CYP gene expression and enzyme activities in the mouse liver. Toxicology. 2007;242:100-8 pubmed
    ..B3 (CVB3) adapted to Balb/c mice was used to examine whether infection affects xenobiotic-metabolising CYP1A1 and CYP2B gene expression (measured by RT-PCR) and the corresponding enzyme activities of ethoxyresorufin-O-deethylase (EROD)..
  29. Yoshinari K, Kobayashi K, Moore R, Kawamoto T, Negishi M. Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital. FEBS Lett. 2003;548:17-20 pubmed
    ..This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin. ..
  30. Noshiro M, Negishi M. Genetic regulation of estrogen-dependent repression of female-specific testosterone 16 alpha-hydroxylase (I-P-450(16 alpha) in male mouse liver: murine Ripr locus. Biochemistry. 1988;27:6444-8 pubmed
    ..The results suggested that estrogen rather than androgen is a repressor of I-P-450(16 alpha) in livers of male mice.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  31. Nemoto N, Sakurai J, Funae Y. Maintenance of phenobarbital-inducible Cyp2b gene expression in C57BL/6 mouse hepatocytes in primary culture as spheroids. Arch Biochem Biophys. 1995;316:362-9 pubmed
    Expression of the Cyp2b-9 and Cyp2b-10 genes was investigated in primary cultured adult C57BL/6NCrj mouse hepatocytes in monolayers or during the formation of spheroids (multicellular aggregates)...
  32. Ryu D, Levi P, Hodgson E. Regulation of cytochrome P-450 isozymes CYP1A1, CYP1A2 and CYP2B10 by three benzodioxole compounds. Chem Biol Interact. 1995;96:235-47 pubmed
    ..used to investigate the structural requirement for regulation of the cytochrome P450 isozymes, CYP1A1, CYP1A2 and CYP2B10, in mouse liver...
  33. Fisher C, Jackson J, Lickteig A, Augustine L, Cherrington N. Drug metabolizing enzyme induction pathways in experimental non-alcoholic steatohepatitis. Arch Toxicol. 2008;82:959-64 pubmed publisher
    ..alters the xenobiotic activation of these transcription factors and induction of downstream DME targets Cyp1A1, Cyp2B10, Cyp3A11, Cyp4A14 and NAD(P)H:quinone oxidoreductase 1 (Nqo1), respectively...
  34. Jarukamjorn K, Sakuma T, Nemoto N. Sexual dimorphic expression of mouse hepatic CYP2B: alterations during development or after hypophysectomy. Biochem Pharmacol. 2002;63:2037-41 pubmed
    ..During the maturation stage, CYP2B10 was expressed in both sexes, whereas CYP2B9 was diminished markedly in the males, resulting in a sexually ..
  35. Honkakoski P, Kojo A, Lang M. Regulation of the mouse liver cytochrome P450 2B subfamily by sex hormones and phenobarbital. Biochem J. 1992;285 ( Pt 3):979-83 pubmed
    ..The fact that P4502b-9 does not respond to phenobarbital, while P4502b-10 is regulated by both phenobarbital and sex hormones, demonstrates the complexity of P450 expression even within one subfamily. ..