Gene Symbol: Cyp1a2
Description: cytochrome P450, family 1, subfamily a, polypeptide 2
Alias: CP12, CYPIA2, P450-3, cytochrome P450 1A2, cholesterol 25-hydroxylase, cytochrome P450 family 1 subfamily a polypeptide 1, cytochrome P450, 1a2, aromatic compound inducible, cytochrome P450-P2, cytochrome P450-P3
Species: mouse
Products:     Cyp1a2

Top Publications

  1. Nukaya M, Moran S, Bradfield C. The role of the dioxin-responsive element cluster between the Cyp1a1 and Cyp1a2 loci in aryl hydrocarbon receptor biology. Proc Natl Acad Sci U S A. 2009;106:4923-8 pubmed publisher
    ..The Cyp1a1 and Cyp1a2 loci represent linked genes thought to play important roles in AHR biology...
  2. Liang H, Li H, McKinnon R, Duffy J, Potter S, Puga A, et al. Cyp1a2(-/-) null mutant mice develop normally but show deficient drug metabolism. Proc Natl Acad Sci U S A. 1996;93:1671-6 pubmed
    b>Cytochrome P450 1A2 (CYP1A2) is a predominantly hepatic enzyme known to be important in the metabolism of numerous foreign chemicals of pharmacologic, toxicologic, and carcinogenic significance...
  3. Dragin N, Dalton T, Miller M, Shertzer H, Nebert D. For dioxin-induced birth defects, mouse or human CYP1A2 in maternal liver protects whereas mouse CYP1A1 and CYP1B1 are inconsequential. J Biol Chem. 2006;281:18591-600 pubmed
    ..The Cyp1a1, Cyp1a2, and Cyp1b1 genes are up-regulated by the aryl hydrocarbon receptor...
  4. Nebert D, Dalton T, Okey A, Gonzalez F. Role of aryl hydrocarbon receptor-mediated induction of the CYP1 enzymes in environmental toxicity and cancer. J Biol Chem. 2004;279:23847-50 pubmed
    The mammalian CYP1A1, CYP1A2, and CYP1B1 genes (encoding cytochromes P450 1A1, 1A2, and 1B1, respectively) are regulated by the aromatic hydrocarbon receptor (AHR)...
  5. Smialowicz R, Burgin D, Williams W, Diliberto J, Setzer R, Birnbaum L. CYP1A2 is not required for 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced immunosuppression. Toxicology. 2004;197:15-22 pubmed
    ..occurs in concert with hepatomegaly and associated induction of several hepatic cytochrome P450 enzymes, including CYP1A2 which is responsible for the hepatic sequestration of TCDD...
  6. Okino S, Pendurthi U, Tukey R. Phorbol esters inhibit the dioxin receptor-mediated transcriptional activation of the mouse Cyp1a-1 and Cyp1a-2 genes by 2,3,7,8-tetrachlorodibenzo-p-dioxin. J Biol Chem. 1992;267:6991-8 pubmed
    ..Analysis of nuclear [3H]TCDD dioxin receptor by sucrose density gradients demonstrated that the inhibition of Cyp1a gene transcription and DNA binding by TPA resulted from a reduction in nuclear dioxin receptor concentration. ..
  7. Bansal S, Leu A, Gonzalez F, Guengerich F, Chowdhury A, Anandatheerthavarada H, et al. Mitochondrial targeting of cytochrome P450 (CYP) 1B1 and its role in polycyclic aromatic hydrocarbon-induced mitochondrial dysfunction. J Biol Chem. 2014;289:9936-51 pubmed publisher
  8. Zhang W, Moorthy B, Chen M, Muthiah K, Coffee R, Purchio A, et al. A Cyp1a2-luciferase transgenic CD-1 mouse model: responses to aryl hydrocarbons similar to the humanized AhR mice. Toxicol Sci. 2004;82:297-307 pubmed
    Here we describe a transgenic mouse model [Crl:CD-1(ICR)BR-Tg(Cyp1a2-luc)Xen] using luciferase as a reporter for Cyp1a2 gene regulation. An 8...
  9. Degawa M, Kobayashi K, Miura S, Arai H, Esumi H, Sugimura T, et al. Species difference among experimental rodents in induction of P450IA family enzymes by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine. Jpn J Cancer Res. 1992;83:1047-51 pubmed
    ..In rats, PhIP induced P450IA1, P450IA2 and a new but unspecified P450 isozyme in the liver, and induced P450IA1 in the kidney. However, PhIP induced none of these P450 isozymes in mice, hamsters and guinea pigs. ..

More Information


  1. Raffalli Mathieu F, Geneste O, Lang M. Characterization of two nuclear proteins that interact with cytochrome P-450 1A2 mRNA. Regulation of RNA binding and possible role in the expression of the Cyp1a2 gene. Eur J Biochem. 1997;245:17-24 pubmed
    Regulation of the expression of the cytochrome P-450 la2 gene (cyp1a2) occurs mainly at the transcriptional level, but the molecular events involved in the induction process are partly unknown...
  2. Yanagida A, Chikada H, Ito K, Umino A, Kato Itoh M, Yamazaki Y, et al. Liver maturation deficiency in p57(Kip2)-/- mice occurs in a hepatocytic p57(Kip2) expression-independent manner. Dev Biol. 2015;407:331-43 pubmed publisher
    ..Furthermore, p57(Kip2)-/- hepatoblasts could differentiate into mature hepatocytes in p57(Kip2)-/- and WT chimeric mice, suggesting that the intrinsic activity of p57(Kip2) does not simply regulate hepatoblast maturation. ..
  3. Dragin N, Uno S, Wang B, Dalton T, Nebert D. Generation of 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-) mouse line. Biochem Biophys Res Commun. 2007;359:635-42 pubmed
    Human/rodent CYP1A1 and CYP1A2 orthologs are well known to exhibit species-specific differences in substrate preferences and rates of metabolism...
  4. Ward J, Yoon M, Anver M, Haines D, Kudo G, Gonzalez F, et al. Hyalinosis and Ym1/Ym2 gene expression in the stomach and respiratory tract of 129S4/SvJae and wild-type and CYP1A2-null B6, 129 mice. Am J Pathol. 2001;158:323-32 pubmed
    ..cytoplasmic change) in the glandular stomach, respiratory tract, bile duct, and gall bladder of B6,129 CYP1A2-null and wild-type mice as well as in both sexes of the background 129S4/SvJae strain...
  5. Senft A, Dalton T, Nebert D, Genter M, Puga A, Hutchinson R, et al. Mitochondrial reactive oxygen production is dependent on the aromatic hydrocarbon receptor. Free Radic Biol Med. 2002;33:1268-78 pubmed
    ..reactive oxygen production on the aromatic hydrocarbon receptor (AHR), cytochrome P450 1A1 (CYP1A1), and cytochrome P450 1A2 (CYP1A2), proteins believed to be important in dioxin-induced liver toxicity...
  6. Uno S, Wang B, Shertzer H, Nebert D, Dalton T. Balancer-Cre transgenic mouse germ cells direct the incomplete resolution of a tri-loxP-targeted Cyp1a1 allele, producing a conditional knockout allele. Biochem Biophys Res Commun. 2003;312:494-9 pubmed
    ..Removal of the marker gene resulted in a conditional Cyp1a1 allele whose expression was indistinguishable from that of the wild-type allele. ..
  7. Mikstacka R, Rimando A, Szalaty K, Stasik K, Baer Dubowska W. Effect of natural analogues of trans-resveratrol on cytochromes P4501A2 and 2E1 catalytic activities. Xenobiotica. 2006;36:269-85 pubmed
    ..the inhibitory effect of a series of naturally occurring trans-resveratrol analogues on cytochromes P450, namely CYP1A2 and CYP2E1, in vitro in order to analyse any structure-activity relationships...
  8. Pendurthi U, Okino S, Tukey R. Accumulation of the nuclear dioxin (Ah) receptor and transcriptional activation of the mouse Cyp1a-1 and Cyp1a-2 genes. Arch Biochem Biophys. 1993;306:65-9 pubmed
  9. Casley W, Menzies J, Mousseau N, Girard M, Moon T, Whitehouse L. Increased basal expression of hepatic Cyp1a1 and Cyp1a2 genes in inbred mice selected for susceptibility to acetaminophen-induced hepatotoxicity. Pharmacogenetics. 1997;7:283-93 pubmed
    ..Microsomal cytochrome P450 activities associated with CYP1A2 (acetanilide 4-hydroxylation and methoxyresorufin O-demethylase) were significantly increased in the susceptible ..
  10. Cheung C, Loy S, Li G, Liu A, Yang C. Rapid induction of colon carcinogenesis in CYP1A-humanized mice by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and dextran sodium sulfate. Carcinogenesis. 2011;32:233-9 pubmed publisher
    ..Metabolic activation of PhIP is primarily mediated by the enzyme cytochrome P450 (CYP) 1A2. Metabolism of PhIP by CYP1A2 differs considerably between humans and rodents, with more N(2)-hydroxylation (activation) and less 4'-..
  11. Liang H, McKinnon R, Nebert D. Sensitivity of CYP1A1 mRNA inducibility by dioxin is the same in Cyp1a2(+/+) wild-type and Cyp1a2(-/-) null mutant mice. Biochem Pharmacol. 1997;54:1127-31 pubmed
    ..experimental porphyria by halogenated aromatic hydrocarbons (HAHs) seems to be influenced by the levels of hepatic CYP1A2. The pharmacokinetics and relative rates of uptake and storage of HAHs in the liver are correlated with hepatic ..
  12. Gonzalez F, Mackenzie P, Kimura S, Nebert D. Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450. Gene. 1984;29:281-92 pubmed
    ..The 3' terminal exon of P1-450 is about 500 bp longer than that of P3-450. These data suggest that both P1-450 and P3-450 have diverged from a common ancestral gene. ..
  13. Jin M, Dewa Y, Kawai M, Nishimura J, Saegusa Y, Kemmochi S, et al. The threshold dose for liver tumor promoting effects of dicyclanil in ICR mice. J Toxicol Sci. 2010;35:69-78 pubmed
    ..Real-time polymerase chain reaction (RT-PCR) showed that the expression of Cyp1a1, Cyp1a2, and OGG1genes was significantly up-regulated in mice given diets containing 375 ppm DC or more, 187...
  14. Genter M, Marlowe J, Kevin Kerzee J, Dragin N, Puga A, Dalton T, et al. Naphthalene toxicity in mice and aryl hydrocarbon receptor-mediated CYPs. Biochem Biophys Res Commun. 2006;348:120-3 pubmed
    ..enzymes are widely regarded as responsible for NP bioactivation, other metabolic enzymes--including CYP1A1 and CYP1A2--produce NP-1,2-oxide in vitro...
  15. Hildebrand C, Stallings R, Gonzalez F, Nebert D. Assignment of dioxin-inducible cytochrome P-450 gene family to Chinese hamster chromosome 4. Somat Cell Mol Genet. 1985;11:391-5 pubmed
    ..0 and 7.4 kb) were assigned to Chinese hamster chromosome 4. These data are consistent with linkage conservation among these two P-450 sequences and four other loci on mouse chromosome 9 that map to hamster chromosome 4. ..
  16. Tuteja N, Gonzalez F, Nebert D. Developmental and tissue-specific differential regulation of the mouse dioxin-inducible P1-450 and P3-450 genes. Dev Biol. 1985;112:177-84 pubmed
  17. Genter M, Clay C, Dalton T, Dong H, Nebert D, Shertzer H. Comparison of mouse hepatic mitochondrial versus microsomal cytochromes P450 following TCDD treatment. Biochem Biophys Res Commun. 2006;342:1375-81 pubmed
    TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) induces cytochromes P450 (CYPs) such as CYP1A1 and CYP1A2 via activation of the aromatic hydrocarbon receptor (AHR)...
  18. Nemoto N, Sakurai J. Activation of Cyp1a1 and Cyp1a2 genes in adult mouse hepatocytes in primary culture. Jpn J Cancer Res. 1993;84:272-8 pubmed
    Expression of Cyp1a1 and Cyp1a2 genes was investigated in adult C57BL/6NCrj mouse hepatocytes in primary culture for up to 5 days...
  19. Kondraganti S, Jiang W, Moorthy B. Differential regulation of expression of hepatic and pulmonary cytochrome P4501A enzymes by 3-methylcholanthrene in mice lacking the CYP1A2 gene. J Pharmacol Exp Ther. 2002;303:945-51 pubmed
    ..In this study, we tested the hypothesis that CYP1A2, a liver-specific P450 isozyme, plays an important role in the mechanisms governing persistent CYP1A1 induction by ..
  20. Cheng K, Park S, Krutzsch H, Grantham P, Gelboin H, Friedman F. Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450. Biochemistry. 1986;25:2397-402 pubmed
    ..This report shows the varying degree of structural relatedness among the isozymes examined and demonstrates the suitability and advantage of immunopurified cytochromes P-450 for sequencing and structural studies. ..
  21. Ryu D, Levi P, Hodgson E. Regulation of cytochrome P-450 isozymes CYP1A1, CYP1A2 and CYP2B10 by three benzodioxole compounds. Chem Biol Interact. 1995;96:235-47 pubmed
    ..were used to investigate the structural requirement for regulation of the cytochrome P450 isozymes, CYP1A1, CYP1A2 and CYP2B10, in mouse liver...
  22. Smith A, Clothier B, Carthew P, Childs N, Sinclair P, Nebert D, et al. Protection of the Cyp1a2(-/-) null mouse against uroporphyria and hepatic injury following exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Appl Pharmacol. 2001;173:89-98 pubmed
    ..A variety of evidence implicates the CYP1A2 enzyme as necessary for mouse uroporphyria...
  23. Zaccaro C, Sweitzer S, Pipino S, Gorman N, Sinclair P, Sinclair J, et al. Role of cytochrome P450 1A2 in bilirubin degradation Studies in Cyp1a2 (-/-) mutant mice. Biochem Pharmacol. 2001;61:843-9 pubmed
    ..CYP1A1 to CYP1A2 ratios were greater in rats treated with BNF...
  24. Yamazaki K, Mizui Y, Sagane K, Tanaka I. Assignment of a disintegrin and metalloproteinase domain 10 (Adam10) gene to mouse chromosome 9. Genomics. 1997;46:528-9 pubmed
  25. Shertzer H, Genter M, Talaska G, Curran C, Nebert D, Dalton T. 7H-dibenzo[c,g]carbazole metabolism by the mouse and human CYP1 family of enzymes. Carcinogenesis. 2007;28:1371-8 pubmed
    ..Using knockout mice for Cyp1a1, Cyp1a2 and Cyp1b1, we showed that the major CYP1 enzymes that metabolize DBC are CYP1A1 in beta-naphthoflavone (BNF)-..
  26. Dragin N, Shi Z, Madan R, Karp C, Sartor M, Chen C, et al. Phenotype of the Cyp1a1/1a2/1b1-/- triple-knockout mouse. Mol Pharmacol. 2008;73:1844-56 pubmed publisher
  27. Jaradat S, Ko M, Grossman L. Tissue-specific expression and mapping of the Cox7ah gene in mouse. Genomics. 1998;49:363-70 pubmed
    ..1. As a result, all three COX heart isoform genes in mouse group to chromosome 7. Interestingly, mapping of the mouse Cox7al to chromosome 9 suggests a new syntenic region between the mouse and the human genomes. ..
  28. Klinefelter K, Hooven M, Bates C, Colter B, Dailey A, Infante S, et al. Genetic differences in the aryl hydrocarbon receptor and CYP1A2 affect sensitivity to developmental polychlorinated biphenyl exposure in mice: relevance to studies of human neurological disorders. Mamm Genome. 2018;29:112-127 pubmed publisher
    ..Our previous studies found allelic differences in the aryl hydrocarbon receptor and cytochrome P450 1A2 (CYP1A2) affect sensitivity to developmental PCB exposure, resulting in cognitive deficits and motor ..
  29. Diliberto J, Burgin D, Birnbaum L. Role of CYP1A2 in hepatic sequestration of dioxin: studies using CYP1A2 knock-out mice. Biochem Biophys Res Commun. 1997;236:431-3 pubmed
    ..Transgenic mice lacking a cytochrome P450 1A2 gene were used to study the influence of the CYP1A2 gene on the hepatic sequestration and distribution of ..
  30. Corcos L, Marc N, Wein S, Fautrel A, Guillouzo A, Pineau T. Phenobarbital induces cytochrome P4501A2 hnRNA, mRNA and protein in the liver of C57BL/6J wild type and aryl hydrocarbon receptor knock-out mice. FEBS Lett. 1998;425:293-7 pubmed
    ..Using the DNA binding site for the aryl hydrocarbon receptor as a probe, gel retardation analyses showed that phenobarbital treatment induced protein binding, regardless of the presence of the aryl hydrocarbon receptor. ..
  31. Uchida Y, Yano A, Kumakura S, Sakuma T, Nemoto N. Enhancer elements in the mouse Cyp1a2 gene for constitutive expression. Biochem Biophys Res Commun. 2002;297:1297 pubmed
    b>CYP1A2 is one of the major hepatic cytochrome P450s that is involved in the metabolism of many drugs, as well as in the activation of chemical carcinogens...
  32. Curran C, Nebert D, Genter M, Patel K, Schaefer T, Skelton M, et al. In utero and lactational exposure to PCBs in mice: adult offspring show altered learning and memory depending on Cyp1a2 and Ahr genotypes. Environ Health Perspect. 2011;119:1286-93 pubmed publisher
    ..Humans genetically have > 60-fold differences in hepatic cytochrome P450 1A2 (CYP1A2)-uninduced basal levels and > 12-fold variability in aryl hydrocarbon receptor (AHR)affinity; ..
  33. Peters J, Morishima H, Ward J, Coakley C, Kimura S, Gonzalez F. Role of CYP1A2 in the toxicity of long-term phenacetin feeding in mice. Toxicol Sci. 1999;50:82-9 pubmed
    ..In particular, it is not known whether these effects are mediated by metabolic activation of the drug. CYP1A2 is known to metabolize phenacetin in vitro...
  34. Diliberto J, Burgin D, Birnbaum L. Effects of CYP1A2 on disposition of 2,3,7, 8-tetrachlorodibenzo-p-dioxin, 2,3,4,7,8-pentachlorodibenzofuran, and 2,2',4,4',5,5'-hexachlorobiphenyl in CYP1A2 knockout and parental (C57BL/6N and 129/Sv) strains of mice. Toxicol Appl Pharmacol. 1999;159:52-64 pubmed
    ..responsible for this hepatic sequestration of TCDD and its congeners has been shown by our laboratory to be CYP1A2 (J. J. Diliberto, D. Burgin, and L. S. Birnbaum, 1997, Biochem. Biophys. Res. Commun. 236, 431-433)...
  35. Prochazka M, Leiter E, Serreze D, Coleman D. Three recessive loci required for insulin-dependent diabetes in nonobese diabetic mice. Science. 1987;237:286-9 pubmed
  36. CURRAN C, Altenhofen E, Ashworth A, Brown A, Kamau Cheggeh C, Curran M, et al. Ahrd Cyp1a2(-/-) mice show increased susceptibility to PCB-induced developmental neurotoxicity. Neurotoxicology. 2012;33:1436-42 pubmed publisher
    ..Coplanar PCBs bind the aryl hydrocarbon receptor (AHR) and can be sequestered in liver by cytochrome P450 1A2 (CYP1A2)...
  37. Giguere V, Beatty B, Squire J, Copeland N, Jenkins N. The orphan nuclear receptor ROR alpha (RORA) maps to a conserved region of homology on human chromosome 15q21-q22 and mouse chromosome 9. Genomics. 1995;28:596-8 pubmed
    ..This places the human RORA gene in the proximity of the PML gene, which is involved in a reciprocal chromosomal translocation t(15:17) with the RARA gene in patients with acute promyelocytic leukemia. ..
  38. Gorman N, Ross K, Walton H, Bement W, Szakacs J, Gerhard G, et al. Uroporphyria in mice: thresholds for hepatic CYP1A2 and iron. Hepatology. 2002;35:912-21 pubmed
    ..Here we investigate the development of uroporphyria as a function of CYP1A2 and iron levels in the liver of mice having a common C57BL/6 genetic background...
  39. Pineau T, Fernandez Salguero P, Lee S, McPhail T, Ward J, Gonzalez F. Neonatal lethality associated with respiratory distress in mice lacking cytochrome P450 1A2. Proc Natl Acad Sci U S A. 1995;92:5134-8 pubmed
    b>Cytochrome P450 1A2 (CYP1A2) is a constitutively expressed hepatic enzyme that is highly conserved among mammals...
  40. Planas Paz L, Orsini V, Boulter L, Calabrese D, Pikiolek M, Nigsch F, et al. The RSPO-LGR4/5-ZNRF3/RNF43 module controls liver zonation and size. Nat Cell Biol. 2016;18:467-79 pubmed publisher
    ..Taken together, our results indicate that the RSPO-LGR4/5-ZNRF3/RNF43 module controls metabolic liver zonation and is a hepatic growth/size rheostat during development, homeostasis and regeneration. ..
  41. Tsyrlov I, Goldfarb I, Gelboin H. Enzyme-kinetic and immunochemical characteristics of mouse cDNA-expressed, microsomal, and purified CYP1A1 and CYP1A2. Arch Biochem Biophys. 1993;307:259-66 pubmed
    ..were estimated in microsomes of Hep G2 cells infected with a recombinant vaccinia virus bearing mouse CYP1A1 or CYP1A2 cDNAs...
  42. Gonzalez F, Kimura S. Role of gene knockout mice in understanding the mechanisms of chemical toxicity and carcinogenesis. Cancer Lett. 1999;143:199-204 pubmed
    ..Mouse lines having disruption of genes encoding P450s CYP1A2, CYP2E1, and CYP1B1 were developed by use of gene disruption in empbryonic stem cells...
  43. McAvoy M, Singh A, Shichi H. In situ hybridization of Cyp1a1, Cyp1a2 and Ah receptor mRNAs expressed in murine ocular tissues. Exp Eye Res. 1996;62:449-52 pubmed
  44. Peterson T, Gonzalez F, Nebert D. Methylation differences in the murine P1450 and P3450 genes in wild-type and mutant hepatoma cell culture. Biochem Pharmacol. 1986;35:2107-14 pubmed
    ..It is of interest that the same nine bases are found in intron 2 about 80 bp upstream from the 5' end of exon 3 in the homologous P3450 gene. ..
  45. Miller R, Ozaki J, Riblet R, Gold D. Genetic mapping of mouse T3d and T3e between Apoa1 and Ncam. Immunogenetics. 1989;30:511-4 pubmed
  46. Prochazka M, Serreze D, Worthen S, Leiter E. Genetic control of diabetogenesis in NOD/Lt mice. Development and analysis of congenic stocks. Diabetes. 1989;38:1446-55 pubmed
    ..abstract truncated at 400 words) ..
  47. Chaloupka K, Santostefano M, Goldfarb I, Liu G, Myers M, Tsyrolv I, et al. Aryl hydrocarbon (Ah) receptor-independent induction of Cyp1a2 gene expression by acenaphthylene and related compounds in B6C3F1 mice. Carcinogenesis. 1994;15:2835-40 pubmed
    ..Acenaphthylene was the most potent inducer of MROD, a Cyp1a2-dependent activity, and was utilized as a prototypical inducer for this group of tricyclic hydrocarbons...
  48. Hildebrand C, Gonzalez F, Kozak C, Nebert D. Regional linkage analysis of the dioxin-inducible P-450 gene family on mouse chromosome 9. Biochem Biophys Res Commun. 1985;130:396-406 pubmed
  49. Jin B, Ryu D. Regulation of CYP1A2 by histone deacetylase inhibitors in mouse hepatocytes. J Biochem Mol Toxicol. 2004;18:131-2 pubmed
    b>Cytochrome P450 1A2 (CYP1A2) is constitutively expressed in the mouse liver, but the constitutive expression progressively declines to an undetectable level in isolated hepatocytes...
  50. Wydner K, Kim Y, Csiszar K, Boyd C, Passmore H. An intron capture strategy used to identify and map a lysyl oxidase-like gene on chromosome 9 in the mouse. Genomics. 1997;40:342-5 pubmed
  51. Uno S, Endo K, Ishida Y, Tateno C, Makishima M, Yoshizato K, et al. CYP1A1 and CYP1A2 expression: comparing 'humanized' mouse lines and wild-type mice; comparing human and mouse hepatoma-derived cell lines. Toxicol Appl Pharmacol. 2009;237:119-26 pubmed publisher
    ..mouse, carrying the human CYP1A1_CYP1A2 locus and lacking the mouse Cyp1a1 and Cyp1a2 orthologs, was shown to express robustly human dioxin-inducible CYP1A1 and basal versus inducible CYP1A2 (mRNAs, ..
  52. McKinnon R, Nebert D. Cytochrome P450 knockout mice: new toxicological models. Clin Exp Pharmacol Physiol. 1998;25:783-7 pubmed
    ..4. Also discussed are null mouse lines with targeted mutations in genes encoding receptors having primary roles in Cyp gene regulation. ..
  53. Uno S, Dalton T, Dragin N, Curran C, Derkenne S, Miller M, et al. Oral benzo[a]pyrene in Cyp1 knockout mouse lines: CYP1A1 important in detoxication, CYP1B1 metabolism required for immune damage independent of total-body burden and clearance rate. Mol Pharmacol. 2006;69:1103-14 pubmed
    ..To further investigate the mechanism for this enhanced sensitivity, Cyp1a1-/-, Cyp1a2-/-, and Cyp1b1-/- single-knockout, Cyp1a1/1b1-/- and Cyp1a2/1b1-/- double-knockout, and Cyp1+/+ wild-type mice ..
  54. Brooks R, Gooderham N, Zhao K, Edwards R, Howard L, Boobis A, et al. 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine is a potent mutagen in the mouse small intestine. Cancer Res. 1994;54:1665-71 pubmed
    ..Immunocytochemical localization of the P-450 enzyme CYP1A2, which is responsible for the primary activation of these mutagens, shows that in untreated mice it is present in ..
  55. Udomsuk L, Juengwatanatrakul T, Putalun W, Jarukamjorn K. Bimodal action of miroestrol and deoxymiroestrol, phytoestrogens from Pueraria candollei var. mirifica, on hepatic CYP2B9 and CYP1A2 expressions and antilipid peroxidation in mice. Nutr Res. 2012;32:45-51 pubmed publisher
    ..We hypothesized that miroestrol and deoxymiroestrol would induce CYP2B9 expression, whereas CYP1A2 expression would be suppressed compared with estradiol...
  56. Montagutelli X, Serikawa T, Guenet J. PCR-analyzed microsatellites: data concerning laboratory and wild-derived mouse inbred strains. Mamm Genome. 1991;1:255-9 pubmed
    ..These results emphasize the interest of using Mus musculus musculus inbred strains to establish interspecific crosses, particularly when considering their breeding performances. ..
  57. Dalton T, Dieter M, Matlib R, Childs N, Shertzer H, Genter M, et al. Targeted knockout of Cyp1a1 gene does not alter hepatic constitutive expression of other genes in the mouse [Ah] battery. Biochem Biophys Res Commun. 2000;267:184-9 pubmed
    ..In a cultured hepatoma CYP1A1 metabolism-deficient mutant line that does not express Cyp1a2, we have previously shown that constitutive transcriptional up-regulation of other [Ah] gene battery members ..
  58. Chen X, Sun C, Han G, Peng J, Li Y, Liu Y, et al. Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression. World J Gastroenterol. 2009;15:1829-35 pubmed
    ..Hepatic CYP2E1 and CYP1A2 protein and mRNA expression levels were evaluated by Western blotting, immunohistochemical staining and ..
  59. Casley W, Menzies J, Whitehouse L, Moon T. Detection of quantitative trait loci affecting caffeine metabolism by interval mapping in a genome-wide scan of C3H/HeJ x APN F(2) mice. Drug Metab Dispos. 1999;27:1375-80 pubmed
    ..0) between a locus on chromosome 9, which colocalized with the murine Cyp1a2 locus, and the plasma paraxanthine/caffeine ratio was identified...
  60. Kimura S, Gonzalez F, Nebert D. The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences. J Biol Chem. 1984;259:10705-13 pubmed
  61. Vaas S, Kreft L, Schwarz M, Braeuning A. Cooperation of structurally different aryl hydrocarbon receptor agonists and β-catenin in the regulation of CYP1A expression. Toxicology. 2014;325:31-41 pubmed publisher
  62. Lingappan K, Jiang W, Wang L, Couroucli X, Moorthy B. Sex-specific differences in hyperoxic lung injury in mice: role of cytochrome P450 (CYP)1A. Toxicology. 2015;331:14-23 pubmed publisher
    ..Eight week-old male and female wild type (WT) (C57BL/6J) mice, Cyp1a1-/-, and Cyp1a2-/- mice were exposed to 72h of hyperoxia (FiO2>0.95)...
  63. Zaher H, Buters J, Ward J, Bruno M, Lucas A, Stern S, et al. Protection against acetaminophen toxicity in CYP1A2 and CYP2E1 double-null mice. Toxicol Appl Pharmacol. 1998;152:193-9 pubmed
    ..At least two forms of cytochrome P450, CYP2E1 and CYP1A2, have been implicated in this reaction in mice...
  64. Stiborova M, Levová K, Bárta F, Shi Z, Frei E, Schmeiser H, et al. Bioactivation versus detoxication of the urothelial carcinogen aristolochic acid I by human cytochrome P450 1A1 and 1A2. Toxicol Sci. 2012;125:345-58 pubmed publisher
    ..human cytochrome P450 (CYP) 1A1 and 1A2 in two CYP1A-humanized mouse lines that carry functional human CYP1A1 and CYP1A2 genes in the absence of the mouse Cyp1a1/1a2 orthologs...
  65. Kimura S, Nebert D. cDNA and complete amino acid sequence of mouse P2(450): allelic variant of mouse P3(450) gene. Nucleic Acids Res. 1986;14:6765-6 pubmed
  66. Brott B, Alessandrini A, Largaespada D, Copeland N, Jenkins N, Crews C, et al. MEK2 is a kinase related to MEK1 and is differentially expressed in murine tissues. Cell Growth Differ. 1993;4:921-9 pubmed
    ..Recombinant MEK2 produced in bacteria phosphorylates a kinase-inactive Erk-1 on tyrosine and threonine, whereas a kinase-inactive mutant MEK2 does not. These findings suggest that MEK2 is a member of a multigene family. ..
  67. Uno S, Dragin N, Miller M, Dalton T, Gonzalez F, Nebert D. Basal and inducible CYP1 mRNA quantitation and protein localization throughout the mouse gastrointestinal tract. Free Radic Biol Med. 2008;44:570-83 pubmed
    The CYP1A1, CYP1A2, and CYP1B1 enzymes are inducible by benzo[a]pyrene (BaP) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD); metabolism of BaP by these enzymes leads to electrophilic intermediates and genotoxicity...
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    ..based on in vitro enzyme kinetic evidence, had postulated initial N-hydroxylation by the cytochrome P450 isoform CYP1A2. This is followed by phase 2 O-conjugation and hydrolysis to form a reactive nitrenium ion that covalently binds ..
  69. Jiang Z, Dalton T, Jin L, Wang B, Tsuneoka Y, Shertzer H, et al. Toward the evaluation of function in genetic variability: characterizing human SNP frequencies and establishing BAC-transgenic mice carrying the human CYP1A1_CYP1A2 locus. Hum Mutat. 2005;25:196-206 pubmed
    Interindividual differences in human CYP1A1 and CYP1A2 expression appear to be associated with variability in risk toward various types of environmental toxicity and cancer...
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    ..Induction of CYP1A1, CYP1A2, and UGT1*06 mRNAs by the AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin was absent in livers of Arnt-floxed/Mx1-..
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    ..Using interspecies backcrosses, we have localized Tpm-1 on mouse chromosome 9, cen-Cyp1a2-Tpm-1-Mod-1-Mylc-Scn5a, near the d-se region...
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    ..Notably, a C-4 zinc finger type motif is recognized in human and mouse ClpX. This motif of so far unknown function is present only in a subset of the known ClpX sequences. ..
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    ..b>Cyp1a2(-/-) knockout mice were used to compare the effects of CYP1A2 expression on uroporphyria and liver carcinogenesis...
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    ..Except for CYP1A2, all of these CYPs exhibited specific patterns of expression during mouse ontogeny, suggesting possible ..
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    ..Our finding of cerebellum-specific coregulation suggests that distinct sets of target genes regulated by the ROR alpha1 and ROR alpha4 isoforms are required for Purkinje cell development. ..
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    Expression of Cyp1a1 and Cyp1a2 genes was investigated in adult C57BL/6NCrj mouse hepatocytes for up to 5 days after transfer to monolayer culture...
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    ..These data demonstrate conclusively that pBR322 clone 46 DNA is associated with mRNA controlled by the murine Ah locus, presumably the structural gene encoding 3-methylcholanthrene-induced P(1)-450. ..
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    ..In contrast, while levels of CYP1A2 mRNA in spheroid culture were also appreciable throughout, they rapidly decreased in monolayer culture to become ..
  79. Hakk H, Diliberto J, Birnbaum L. The effect of dose on 2,3,7,8-TCDD tissue distribution, metabolism and elimination in CYP1A2 (-/-) knockout and C57BL/6N parental strains of mice. Toxicol Appl Pharmacol. 2009;241:119-26 pubmed publisher
    ..A hallmark feature of TCDD exposure is induction of hepatic CYP1A2 and subsequent sequestration leading to high liver-to-fat concentration ratios...
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    Cytochrome P4501A2 (Cyp1a2) is important in the development of uroporphyria in mice, a model of porphyria cutanea tarda in humans...
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    ..generation of the hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahr(d) mouse line, which carries human functional CYP1A1 and CYP1A2 genes in the absence of mouse Cyp1a1 and Cyp1a2 genes, in a (>99...
  82. Levová K, Moserova M, Nebert D, Phillips D, Frei E, Schmeiser H, et al. NAD(P)H:quinone oxidoreductase expression in Cyp1a-knockout and CYP1A-humanized mouse lines and its effect on bioactivation of the carcinogen aristolochic acid I. Toxicol Appl Pharmacol. 2012;265:360-7 pubmed publisher
    ..We found that NQO1 levels in liver, kidney and lung of Cyp1a1?/?, Cyp1a2?/? and Cyp1a1/1a2?/? knockout mouse lines, as well as in two CYP1A-humanized mouse lines harboring functional ..
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    The aim of this study was to use the CYP1A2-null mouse to investigate the in-vivo contribution of CYP1A2 to clozapine pharmacokinetics and pharmacodynamics...
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    Using Cyp1a2(-/-) mice we previously showed that CYP1A2 is absolutely required for hepatic uroporphyrin accumulation caused by iron and 5-aminolevulinate (ALA) treatment, both in the presence and absence of an inducer of CYP1A2...
  85. Wang L, Lingappan K, Jiang W, Couroucli X, Welty S, Shivanna B, et al. Disruption of cytochrome P4501A2 in mice leads to increased susceptibility to hyperoxic lung injury. Free Radic Biol Med. 2015;82:147-59 pubmed publisher
    ..Cytochrome P450 (CYP) 1A enzymes have been implicated in hyperoxic lung injury, but the mechanistic role of CYP1A2 in pulmonary injury is not known...
  86. Petersen D, Gonzalez F, Rapic V, Kozak C, Lee J, Jones J, et al. Marked increases in hepatic NAD(P)H:oxidoreductase gene transcription and mRNA levels correlated with a mouse chromosome 7 deletion. Proc Natl Acad Sci U S A. 1989;86:6699-703 pubmed
    ..Conversely, there are no detectable basal levels of cytochrome P1450 (Cyp1a1 gene) or cytochrome P3450 (Cyp1a2 gene) mRNA, indicating that the regulation of basal expression of the Cyp1a1 and Cyp1a2 genes is distinct from ..