Cyp1a2

Summary

Gene Symbol: Cyp1a2
Description: cytochrome P450, family 1, subfamily a, polypeptide 2
Alias: CP12, Cyp1a1, P450-3, CYPIA2, aromatic compound inducible, cytochrome P450 1A2, cytochrome P450 family 1 subfamily a polypeptide 1, cytochrome P450, 1a2, aromatic compound inducible, cytochrome P450-P2, cytochrome P450-P3
Species: mouse

Top Publications

  1. ncbi Role of aryl hydrocarbon receptor-mediated induction of the CYP1 enzymes in environmental toxicity and cancer
    Daniel W Nebert
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0056, USA
    J Biol Chem 279:23847-50. 2004
  2. ncbi For dioxin-induced birth defects, mouse or human CYP1A2 in maternal liver protects whereas mouse CYP1A1 and CYP1B1 are inconsequential
    Nadine Dragin
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0056, USA
    J Biol Chem 281:18591-600. 2006
  3. pmc Cyp1a2(-/-) null mutant mice develop normally but show deficient drug metabolism
    H C Liang
    Department of Environmental Health, University of Cincinnati Medical Center, OH 45267 0056, USA
    Proc Natl Acad Sci U S A 93:1671-6. 1996
  4. doi NAD(P)H:quinone oxidoreductase expression in Cyp1a-knockout and CYP1A-humanized mouse lines and its effect on bioactivation of the carcinogen aristolochic acid I
    Katerina Levová
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Toxicol Appl Pharmacol 265:360-7. 2012
  5. pmc Uroporphyria in the Cyp1a2-/- mouse
    John D Phillips
    University of Utah School of Medicine, Department of Internal Medicine, Division of Hematology, Salt Lake City, UT 84132, USA
    Blood Cells Mol Dis 47:249-54. 2011
  6. pmc Bioactivation versus detoxication of the urothelial carcinogen aristolochic acid I by human cytochrome P450 1A1 and 1A2
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, 12840 Prague 2, Czech Republic
    Toxicol Sci 125:345-58. 2012
  7. doi Bimodal action of miroestrol and deoxymiroestrol, phytoestrogens from Pueraria candollei var. mirifica, on hepatic CYP2B9 and CYP1A2 expressions and antilipid peroxidation in mice
    Latiporn Udomsuk
    Academic Office for Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
    Nutr Res 32:45-51. 2012
  8. pmc Ahrd Cyp1a2(-/-) mice show increased susceptibility to PCB-induced developmental neurotoxicity
    CHRISTINE PERDAN CURRAN
    Department of Biological Sciences, Northern Kentucky University, Highland Heights, KY 41076, USA
    Neurotoxicology 33:1436-42. 2012
  9. ncbi Cyp1a1(-/-) male mice: protection against high-dose TCDD-induced lethality and wasting syndrome, and resistance to intrahepatocyte lipid accumulation and uroporphyria
    Shigeyuki Uno
    Department of Environmental Health and Center for Environmental Genetics CEG, University of Cincinnati Medical Center, Cincinnati, OH 45267 0056, USA
    Toxicol Appl Pharmacol 196:410-21. 2004
  10. ncbi Activation of PPAR-alpha in streptozotocin-induced diabetes is essential for resistance against acetaminophen toxicity
    Kartik Shankar
    Department of Toxicology, School of Pharmacy, College of Health Sciences, The University of Louisiana at Monroe, Louisiana 71209 0495, USA
    FASEB J 17:1748-50. 2003

Research Grants

  1. RECEPTOR MEDIATED OXIDATIVE STRESS
    HOWARD SHERTZER; Fiscal Year: 2003
  2. Glutathione in Environmental Toxicity and Disease
    HOWARD SHERTZER; Fiscal Year: 2007
  3. CYP1A1 GENE AND ENVIRONMENTAL TOXICITY
    Daniel Nebert; Fiscal Year: 2005
  4. Sphingolipid signaling in olfactory mucosa
    Mary Beth Genter; Fiscal Year: 2004
  5. Extracellular matrix in chloracetanilide carcinogenesis
    Mary Beth Genter; Fiscal Year: 2004
  6. MOLECULAR GENETICS OF CD TOXICITY
    Daniel Nebert; Fiscal Year: 2004
  7. Molecular Genetics of Cadmium Toxicity
    Daniel W Nebert; Fiscal Year: 2010
  8. PAHs: Balance of Detoxication vs Metabolic Activation
    Daniel Nebert; Fiscal Year: 2009
  9. Molecular Genetics of Cadmium Toxicity
    Daniel Nebert; Fiscal Year: 2009
  10. PAHs: Balance of Detoxication vs Metabolic Activation
    Daniel Nebert; Fiscal Year: 2009

Scientific Experts

Detail Information

Publications72

  1. ncbi Role of aryl hydrocarbon receptor-mediated induction of the CYP1 enzymes in environmental toxicity and cancer
    Daniel W Nebert
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0056, USA
    J Biol Chem 279:23847-50. 2004
    The mammalian CYP1A1, CYP1A2, and CYP1B1 genes (encoding cytochromes P450 1A1, 1A2, and 1B1, respectively) are regulated by the aromatic hydrocarbon receptor (AHR)...
  2. ncbi For dioxin-induced birth defects, mouse or human CYP1A2 in maternal liver protects whereas mouse CYP1A1 and CYP1B1 are inconsequential
    Nadine Dragin
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0056, USA
    J Biol Chem 281:18591-600. 2006
    ..The Cyp1a1, Cyp1a2, and Cyp1b1 genes are up-regulated by the aryl hydrocarbon receptor...
  3. pmc Cyp1a2(-/-) null mutant mice develop normally but show deficient drug metabolism
    H C Liang
    Department of Environmental Health, University of Cincinnati Medical Center, OH 45267 0056, USA
    Proc Natl Acad Sci U S A 93:1671-6. 1996
    b>Cytochrome P450 1A2 (CYP1A2) is a predominantly hepatic enzyme known to be important in the metabolism of numerous foreign chemicals of pharmacologic, toxicologic, and carcinogenic significance...
  4. doi NAD(P)H:quinone oxidoreductase expression in Cyp1a-knockout and CYP1A-humanized mouse lines and its effect on bioactivation of the carcinogen aristolochic acid I
    Katerina Levová
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Toxicol Appl Pharmacol 265:360-7. 2012
    ..We found that NQO1 levels in liver, kidney and lung of Cyp1a1⁻/⁻, Cyp1a2⁻/⁻ and Cyp1a1/1a2⁻/⁻ knockout mouse lines, as well as in two CYP1A-humanized mouse lines ..
  5. pmc Uroporphyria in the Cyp1a2-/- mouse
    John D Phillips
    University of Utah School of Medicine, Department of Internal Medicine, Division of Hematology, Salt Lake City, UT 84132, USA
    Blood Cells Mol Dis 47:249-54. 2011
    Cytochrome P4501A2 (Cyp1a2) is important in the development of uroporphyria in mice, a model of porphyria cutanea tarda in humans...
  6. pmc Bioactivation versus detoxication of the urothelial carcinogen aristolochic acid I by human cytochrome P450 1A1 and 1A2
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, 12840 Prague 2, Czech Republic
    Toxicol Sci 125:345-58. 2012
    ..by human cytochrome P450 (CYP) 1A1 and 1A2 in two CYP1A-humanized mouse lines that carry functional human CYP1A1 and CYP1A2 genes in the absence of the mouse Cyp1a1/1a2 orthologs...
  7. doi Bimodal action of miroestrol and deoxymiroestrol, phytoestrogens from Pueraria candollei var. mirifica, on hepatic CYP2B9 and CYP1A2 expressions and antilipid peroxidation in mice
    Latiporn Udomsuk
    Academic Office for Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
    Nutr Res 32:45-51. 2012
    ..We hypothesized that miroestrol and deoxymiroestrol would induce CYP2B9 expression, whereas CYP1A2 expression would be suppressed compared with estradiol...
  8. pmc Ahrd Cyp1a2(-/-) mice show increased susceptibility to PCB-induced developmental neurotoxicity
    CHRISTINE PERDAN CURRAN
    Department of Biological Sciences, Northern Kentucky University, Highland Heights, KY 41076, USA
    Neurotoxicology 33:1436-42. 2012
    ..Coplanar PCBs bind the aryl hydrocarbon receptor (AHR) and can be sequestered in liver by cytochrome P450 1A2 (CYP1A2)...
  9. ncbi Cyp1a1(-/-) male mice: protection against high-dose TCDD-induced lethality and wasting syndrome, and resistance to intrahepatocyte lipid accumulation and uroporphyria
    Shigeyuki Uno
    Department of Environmental Health and Center for Environmental Genetics CEG, University of Cincinnati Medical Center, Cincinnati, OH 45267 0056, USA
    Toxicol Appl Pharmacol 196:410-21. 2004
    ..In this study, we asked the question how important is the role of CYP1A1 in causing TCDD toxicity...
  10. ncbi Activation of PPAR-alpha in streptozotocin-induced diabetes is essential for resistance against acetaminophen toxicity
    Kartik Shankar
    Department of Toxicology, School of Pharmacy, College of Health Sciences, The University of Louisiana at Monroe, Louisiana 71209 0495, USA
    FASEB J 17:1748-50. 2003
    ..Gel shift assays revealed higher activation of nuclear factor-kappaB in WT-DB mice after APAP treatment. These findings suggest PPAR-alpha activation as a hepatoprotective adaptive response mediating protection against APAP in diabetes...
  11. ncbi Study of P450 function using gene knockout and transgenic mice
    Frank J Gonzalez
    Building 37, Room 3E 24, Laboratory of Metabolism, National Cancer Institute, Bethesda, MD 20892, USA
    Arch Biochem Biophys 409:153-8. 2003
    ..The P450s chosen for study, CYP1A1, CYP1B1, CYP1A2, and CYP2E1, are conserved in mammals and are known to metabolize most toxicants and chemical ..
  12. ncbi Mitochondrial reactive oxygen production is dependent on the aromatic hydrocarbon receptor
    Albert P Senft
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, OH 45267, USA
    Free Radic Biol Med 33:1268-78. 2002
    ..reactive oxygen production on the aromatic hydrocarbon receptor (AHR), cytochrome P450 1A1 (CYP1A1), and cytochrome P450 1A2 (CYP1A2), proteins believed to be important in dioxin-induced liver toxicity...
  13. ncbi Enhancer elements in the mouse Cyp1a2 gene for constitutive expression
    Yo Ichi Uchida
    Department of Toxicology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930 0194, Toyama, Japan
    Biochem Biophys Res Commun 297:1297. 2002
    b>CYP1A2 is one of the major hepatic cytochrome P450s that is involved in the metabolism of many drugs, as well as in the activation of chemical carcinogens...
  14. ncbi Role of cytochrome P450 1A2 in bilirubin degradation Studies in Cyp1a2 (-/-) mutant mice
    C Zaccaro
    Department of Pharmacology, University of Turin Medical School, Via P Giuria 13, 10125, Turin, Italy
    Biochem Pharmacol 61:843-9. 2001
    ..b>CYP1A1 to CYP1A2 ratios were greater in rats treated with BNF...
  15. ncbi Clozapine pharmacokinetics and pharmacodynamics studied with Cyp1A2-null mice
    K J Aitchison
    Section of Clinical Pharmacology, Institute of Psychiatry, London, UK
    J Psychopharmacol 14:353-9. 2000
    The aim of this study was to use the CYP1A2-null mouse to investigate the in-vivo contribution of CYP1A2 to clozapine pharmacokinetics and pharmacodynamics...
  16. pmc Hyalinosis and Ym1/Ym2 gene expression in the stomach and respiratory tract of 129S4/SvJae and wild-type and CYP1A2-null B6, 129 mice
    J M Ward
    Veterinary and Tumor Pathology Section, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Am J Pathol 158:323-32. 2001
    ..cytoplasmic change) in the glandular stomach, respiratory tract, bile duct, and gall bladder of B6,129 CYP1A2-null and wild-type mice as well as in both sexes of the background 129S4/SvJae strain...
  17. ncbi Targeted knockout of Cyp1a1 gene does not alter hepatic constitutive expression of other genes in the mouse [Ah] battery
    T P Dalton
    Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, Ohio, 45267 0056, USA
    Biochem Biophys Res Commun 267:184-9. 2000
    Using the Cre-lox system, we have generated a cytochrome P450 1A1 Cyp1a1(-/-) knockout mouse by deletion of the translated portions of the Cyp1a1 gene...
  18. ncbi Detection of quantitative trait loci affecting caffeine metabolism by interval mapping in a genome-wide scan of C3H/HeJ x APN F(2) mice
    W L Casley
    Research Division, Therapeutic Products Programme, Health Canada
    Drug Metab Dispos 27:1375-80. 1999
    ..0) between a locus on chromosome 9, which colocalized with the murine Cyp1a2 locus, and the plasma paraxanthine/caffeine ratio was identified...
  19. ncbi Role of gene knockout mice in understanding the mechanisms of chemical toxicity and carcinogenesis
    F J Gonzalez
    National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cancer Lett 143:199-204. 1999
    ..Mouse lines having disruption of genes encoding P450s CYP1A2, CYP2E1, and CYP1B1 were developed by use of gene disruption in empbryonic stem cells...
  20. ncbi Regulation of CYP1A2 by histone deacetylase inhibitors in mouse hepatocytes
    Bohwan Jin
    College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    J Biochem Mol Toxicol 18:131-2. 2004
    b>Cytochrome P450 1A2 (CYP1A2) is constitutively expressed in the mouse liver, but the constitutive expression progressively declines to an undetectable level in isolated hepatocytes...
  21. ncbi Toward the evaluation of function in genetic variability: characterizing human SNP frequencies and establishing BAC-transgenic mice carrying the human CYP1A1_CYP1A2 locus
    Zhengwen Jiang
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0056, USA
    Hum Mutat 25:196-206. 2005
    Interindividual differences in human CYP1A1 and CYP1A2 expression appear to be associated with variability in risk toward various types of environmental toxicity and cancer...
  22. pmc Rapid induction of colon carcinogenesis in CYP1A-humanized mice by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and dextran sodium sulfate
    Connie Cheung
    Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 8020, USA
    Carcinogenesis 32:233-9. 2011
    ..Transgenic CYP1A-humanized mice (hCYP1A-mice), which have the human CYP1A1 and CYP1A2 genes but lack the murine orthologs Cyp1a1 and Cyp1a2, provide an excellent opportunity to develop a ..
  23. ncbi The threshold dose for liver tumor promoting effects of dicyclanil in ICR mice
    Meilan Jin
    Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, Tokyo
    J Toxicol Sci 35:69-78. 2010
    ..Real-time polymerase chain reaction (RT-PCR) showed that the expression of Cyp1a1, Cyp1a2, and OGG1genes was significantly up-regulated in mice given diets containing 375 ppm DC or more, 187...
  24. doi The effect of dose on 2,3,7,8-TCDD tissue distribution, metabolism and elimination in CYP1A2 (-/-) knockout and C57BL/6N parental strains of mice
    Heldur Hakk
    USDA ARS Biosciences Research Laboratory, P O Box 5674, Fargo, ND, USA
    Toxicol Appl Pharmacol 241:119-26. 2009
    ..The data suggested that metabolism of TCDD is inherently slow, due principally to CYP1A1, and that hepatic CYP1A2 is not an active participant in the metabolism of TCDD in male mice...
  25. pmc Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression
    Xia Chen
    Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Lvshunkou District, Dalian, Liaoning Province, China
    World J Gastroenterol 15:1829-35. 2009
    ..To investigate the hepatoprotective activity of tea polyphenols (TP) and its relation with cytochrome P450 (CYP450) expression in mice...
  26. pmc CYP1A1 and CYP1A2 expression: comparing 'humanized' mouse lines and wild-type mice; comparing human and mouse hepatoma-derived cell lines
    Shigeyuki Uno
    Department of Biochemistry, Nihon University School of Medicine, 30 1 Oyaguchikami cho, Itabashi ku, Tokyo 173 8610, Japan
    Toxicol Appl Pharmacol 237:119-26. 2009
    ..a bacterial-artificial-chromosome-transgenic mouse, carrying the human CYP1A1_CYP1A2 locus and lacking the mouse Cyp1a1 and Cyp1a2 orthologs, was shown to express robustly human dioxin-inducible CYP1A1 and basal versus inducible ..
  27. pmc The role of the dioxin-responsive element cluster between the Cyp1a1 and Cyp1a2 loci in aryl hydrocarbon receptor biology
    Manabu Nukaya
    McArdle Laboratory for Cancer Research, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 106:4923-8. 2009
    ..The Cyp1a1 and Cyp1a2 loci represent linked genes thought to play important roles in AHR biology...
  28. pmc Phenotype of the Cyp1a1/1a2/1b1-/- triple-knockout mouse
    Nadine Dragin
    Department of Environmental Health, University of Cincinnati Medical Center, P O Box 670056, Cincinnati, OH 45267 0056, USA
    Mol Pharmacol 73:1844-56. 2008
    Crossing the Cyp1a1/1a2(-/-) double-knockout mouse with the Cyp1b1(-/-) single-knockout mouse, we generated the Cyp1a1/1a2/1b1(-/-) triple-knockout mouse...
  29. pmc Mouse lung CYP1A1 catalyzes the metabolic activation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)
    Xiaochao Ma
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, Bethesda, MD 20892, USA
    Carcinogenesis 28:732-7. 2007
    ..PhIP) carcinogenesis is initiated by N(2)-hydroxylation, mediated by several cytochromes P450, including CYP1A1. However, the role of CYP1A1 in PhIP metabolic activation in vivo is unclear...
  30. ncbi Naphthalene toxicity in mice and aryl hydrocarbon receptor-mediated CYPs
    Mary Beth Genter
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati, Cincinnati, OH 45267 0056, USA
    Biochem Biophys Res Commun 348:120-3. 2006
    ..Whereas CYP2F enzymes are widely regarded as responsible for NP bioactivation, other metabolic enzymes--including CYP1A1 and CYP1A2--produce NP-1,2-oxide in vitro...
  31. ncbi Effect of natural analogues of trans-resveratrol on cytochromes P4501A2 and 2E1 catalytic activities
    R Mikstacka
    Department of Pharmaceutical Biochemistry, University of Medical Sciences, Poznan, Poland
    Xenobiotica 36:269-85. 2006
    ..the inhibitory effect of a series of naturally occurring trans-resveratrol analogues on cytochromes P450, namely CYP1A2 and CYP2E1, in vitro in order to analyse any structure-activity relationships...
  32. ncbi Oral benzo[a]pyrene in Cyp1 knockout mouse lines: CYP1A1 important in detoxication, CYP1B1 metabolism required for immune damage independent of total-body burden and clearance rate
    Shigeyuki Uno
    Department of Environmental Health, University of Cincinnati Medical Center, P O Box 670056, Cincinnati, OH 45267 0056, USA
    Mol Pharmacol 69:1103-14. 2006
    b>CYP1A1 and CYP1B1 metabolically activate many polycyclic aromatic hydrocarbons (PAHs), including benzo[a]pyrene, to reactive intermediates associated with toxicity, mutagenesis, and carcinogenesis...
  33. ncbi Evidence for induced microsomal bilirubin degradation by cytochrome P450 2A5
    A edah Abu-Bakar
    National Research Centre for Environmental Toxicology, University of Queensland, 39 Kessels Road, Coopers Plains, 4108 Brisbane, QLD, Australia
    Biochem Pharmacol 70:1527-35. 2005
    ..By contrast, the total cytochrome P450 (CYP) content and the expression of Cyp1a2 were down-regulated by the treatment...
  34. ncbi Uroporphyria and hepatic carcinogenesis induced by polychlorinated biphenyls-iron interaction: absence in the Cyp1a2(-/-) knockout mouse
    Peter Greaves
    MRC Toxicology Unit, University of Leicester, Leicester, UK
    Biochem Biophys Res Commun 331:147-52. 2005
    ..In contrast, uroporphyria was not detected in Cyp1a2(-/-) knockout mice, although expression of CYP1A1 and CYP2B10 was greatly induced...
  35. ncbi Effects of CYP1A2 on disposition of 2,3,7, 8-tetrachlorodibenzo-p-dioxin, 2,3,4,7,8-pentachlorodibenzofuran, and 2,2',4,4',5,5'-hexachlorobiphenyl in CYP1A2 knockout and parental (C57BL/6N and 129/Sv) strains of mice
    J J Diliberto
    National Health Effects and Environmental Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina, 27711, USA
    Toxicol Appl Pharmacol 159:52-64. 1999
    ..responsible for this hepatic sequestration of TCDD and its congeners has been shown by our laboratory to be CYP1A2 (J. J. Diliberto, D. Burgin, and L. S. Birnbaum, 1997, Biochem. Biophys. Res. Commun. 236, 431-433)...
  36. ncbi Role of CYP1A2 in the toxicity of long-term phenacetin feeding in mice
    J M Peters
    Laboratory of Metabolism, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Toxicol Sci 50:82-9. 1999
    ..In particular, it is not known whether these effects are mediated by metabolic activation of the drug. CYP1A2 is known to metabolize phenacetin in vitro...
  37. ncbi Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450
    F J Gonzalez
    Gene 29:281-92. 1984
    ..The 3' terminal exon of P1-450 is about 500 bp longer than that of P3-450. These data suggest that both P1-450 and P3-450 have diverged from a common ancestral gene...
  38. ncbi The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences
    S Kimura
    J Biol Chem 259:10705-13. 1984
    ....
  39. pmc Localization of cytochrome P1-450 and P3-450 genes to mouse chromosome 9
    R H Tukey
    Proc Natl Acad Sci U S A 81:3163-6. 1984
    ..Chromosome segregation analysis of 12 hybrid clones demonstrated that the structural genes for both P1-450 and P3-450 can be assigned to mouse chromosome 9...
  40. pmc Mouse cytochrome P3-450: complete cDNA and amino acid sequence
    S Kimura
    Nucleic Acids Res 12:2917-28. 1984
    ..These data support the likelihood that cysteine 456 is the thiolate ligand to the heme iron in the P3-450 enzyme active-site...
  41. pmc Isolation and characterization of a cloned DNA sequence associated with the murine Ah locus and a 3-methylcholanthrene-induced form of cytochrome P-450
    M Negishi
    Proc Natl Acad Sci U S A 78:800-4. 1981
    ..These data demonstrate conclusively that pBR322 clone 46 DNA is associated with mRNA controlled by the murine Ah locus, presumably the structural gene encoding 3-methylcholanthrene-induced P(1)-450...
  42. ncbi Regional linkage analysis of the dioxin-inducible P-450 gene family on mouse chromosome 9
    C E Hildebrand
    Biochem Biophys Res Commun 130:396-406. 1985
    ....
  43. ncbi Assignment of dioxin-inducible cytochrome P-450 gene family to Chinese hamster chromosome 4
    C E Hildebrand
    Somat Cell Mol Genet 11:391-5. 1985
    ..0 and 7.4 kb) were assigned to Chinese hamster chromosome 4. These data are consistent with linkage conservation among these two P-450 sequences and four other loci on mouse chromosome 9 that map to hamster chromosome 4...
  44. pmc cDNA and complete amino acid sequence of mouse P2(450): allelic variant of mouse P3(450) gene
    S Kimura
    Nucleic Acids Res 14:6765-6. 1986
  45. ncbi Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450
    K C Cheng
    Biochemistry 25:2397-402. 1986
    ..This report shows the varying degree of structural relatedness among the isozymes examined and demonstrates the suitability and advantage of immunopurified cytochromes P-450 for sequencing and structural studies...
  46. ncbi Genetic control of diabetogenesis in NOD/Lt mice. Development and analysis of congenic stocks
    M Prochazka
    Jackson Laboratory, Bar Harbor, ME 04609
    Diabetes 38:1446-55. 1989
    ..abstract truncated at 400 words)..
  47. ncbi Genetic mapping of mouse T3d and T3e between Apoa1 and Ncam
    R D Miller
    Division of Molecular Biology, Medical Biological Institute, La Jolla, CA 92037
    Immunogenetics 30:511-4. 1989
  48. pmc Marked increases in hepatic NAD(P)H:oxidoreductase gene transcription and mRNA levels correlated with a mouse chromosome 7 deletion
    D D Petersen
    Laboratory of Developmental Pharmacology, National Institute of Child Health and Human Development, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 86:6699-703. 1989
    ..Conversely, there are no detectable basal levels of cytochrome P1450 (Cyp1a1 gene) or cytochrome P3450 (Cyp1a2 gene) mRNA, indicating that the regulation of basal expression of the Cyp1a1 and ..
  49. ncbi Methylation differences in the murine P1450 and P3450 genes in wild-type and mutant hepatoma cell culture
    T C Peterson
    Biochem Pharmacol 35:2107-14. 1986
    ..It is of interest that the same nine bases are found in intron 2 about 80 bp upstream from the 5' end of exon 3 in the homologous P3450 gene...
  50. ncbi PCR-analyzed microsatellites: data concerning laboratory and wild-derived mouse inbred strains
    X Montagutelli
    Unite de Genetique des Mammiferes, Institut Pasteur, Paris, France
    Mamm Genome 1:255-9. 1991
    ..These results emphasize the interest of using Mus musculus musculus inbred strains to establish interspecific crosses, particularly when considering their breeding performances...
  51. ncbi Differences in regulation of gene expression between Cyp1a-1 and Cyp1a-2 in adult mouse hepatocytes in primary culture
    N Nemoto
    Department of Experimental Pathology, Cancer Institute, Tokyo, Japan
    Carcinogenesis 13:2249-54. 1992
    ..The expression of 3-methylcholanthrene (MCA)-induced CYP1A1 mRNA remained high during the observation period under both monolayer and spheroid culture conditions...
  52. ncbi Regulation of cytochrome P-450 isozymes CYP1A1, CYP1A2 and CYP2B10 by three benzodioxole compounds
    D Y Ryu
    Department of Toxicology, North Carolina State University, Raleigh 27695, USA
    Chem Biol Interact 96:235-47. 1995
    ..BD) compounds were used to investigate the structural requirement for regulation of the cytochrome P450 isozymes, CYP1A1, CYP1A2 and CYP2B10, in mouse liver...
  53. ncbi 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine is a potent mutagen in the mouse small intestine
    R A Brooks
    Department of Pathology, University of Cambridge, United Kingdom
    Cancer Res 54:1665-71. 1994
    ..Immunocytochemical localization of the P-450 enzyme CYP1A2, which is responsible for the primary activation of these mutagens, shows that in untreated mice it is present in ..
  54. ncbi Accumulation of the nuclear dioxin (Ah) receptor and transcriptional activation of the mouse Cyp1a-1 and Cyp1a-2 genes
    U R Pendurthi
    Department of Pharmacology, UCSD Cancer Center, San Diego, La Jolla 92093
    Arch Biochem Biophys 306:65-9. 1993
    ....
  55. ncbi Role of CYP1A2 in the hepatotoxicity of acetaminophen: investigations using Cyp1a2 null mice
    R P Tonge
    Department of Medicinal Chemistry, University of Washington, Seattle 98195 7631, USA
    Toxicol Appl Pharmacol 153:102-8. 1998
    ..The P450 isoforms thought to be responsible for APAP hepatotoxicity in humans are CYP2E1, CYP1A2, and CYP3A4, and thus, we have investigated the effect of murine Cyp1a2 on APAP hepatotoxicity using Cyp1a2 ..
  56. ncbi Cytochrome P450 knockout mice: new toxicological models
    R A McKinnon
    Centre for Pharmaceutical Research, School of Pharmacy and Medical Sciences, South Australia, Adelaide, Australia
    Clin Exp Pharmacol Physiol 25:783-7. 1998
    ..4. Also discussed are null mouse lines with targeted mutations in genes encoding receptors having primary roles in Cyp gene regulation...
  57. pmc Epithelial V-like antigen (EVA), a novel member of the immunoglobulin superfamily, expressed in embryonic epithelia with a potential role as homotypic adhesion molecule in thymus histogenesis
    M Guttinger
    Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute HSR, Università di Milano at DIBIT HSR, I 20132 Milan, Italy
    J Cell Biol 141:1061-71. 1998
    ..Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis...
  58. ncbi Phenobarbital induces cytochrome P4501A2 hnRNA, mRNA and protein in the liver of C57BL/6J wild type and aryl hydrocarbon receptor knock-out mice
    L Corcos
    INSERM U456, Faculte de Pharmacie, Rennes, France
    FEBS Lett 425:293-7. 1998
    ..Using the DNA binding site for the aryl hydrocarbon receptor as a probe, gel retardation analyses showed that phenobarbital treatment induced protein binding, regardless of the presence of the aryl hydrocarbon receptor...
  59. ncbi Sensitivity of CYP1A1 mRNA inducibility by dioxin is the same in Cyp1a2(+/+) wild-type and Cyp1a2(-/-) null mutant mice
    H C Liang
    Center for Environmental Genetics and Department of Environmental Health, University of Cincinnati Medical Center, OH 45267 0056, USA
    Biochem Pharmacol 54:1127-31. 1997
    ..The differential inducibility of liver CYP1A1 mRNA by dioxin was therefore compared in Cyp1a2(+/+) wild-type mice, Cyp1a2(+/-) heterozygotes, and Cyp1a2(-/-) ..
  60. pmc Uroporphyria produced in mice by iron and 5-aminolaevulinic acid does not occur in Cyp1a2(-/-) null mutant mice
    P R Sinclair
    VA Medical Center, White River Junction, VT 05009, USA
    Biochem J 330:149-53. 1998
    ..5-fold higher than in Cyp1a2(-/-) mice. Treatment with MC increased hepatic CYP1A1 in both mouse lines and hepatic CYP1A2 only in the Cyp1a2(+/+) line, as determined by Western immunoblotting...
  61. ncbi Role of CYP1A2 in hepatic sequestration of dioxin: studies using CYP1A2 knock-out mice
    J J Diliberto
    Experimental Toxicology Division, National Health Effects and Environmental Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Biochem Biophys Res Commun 236:431-3. 1997
    ..Transgenic mice lacking a cytochrome P450 1A2 gene were used to study the influence of the CYP1A2 gene on the hepatic sequestration and distribution of ..
  62. ncbi The structural integrity of ROR alpha isoforms is mutated in staggerer mice: cerebellar coexpression of ROR alpha1 and ROR alpha4
    U Matysiak-Scholze
    German Cancer Research Center DKFZ, Heidelberg
    Genomics 43:78-84. 1997
    ..Our finding of cerebellum-specific coregulation suggests that distinct sets of target genes regulated by the ROR alpha1 and ROR alpha4 isoforms are required for Purkinje cell development...
  63. ncbi Phorbol esters inhibit the dioxin receptor-mediated transcriptional activation of the mouse Cyp1a-1 and Cyp1a-2 genes by 2,3,7,8-tetrachlorodibenzo-p-dioxin
    S T Okino
    Department of Medicine, University of California, San Diego, La Jolla 92093
    J Biol Chem 267:6991-8. 1992
    ..Analysis of nuclear [3H]TCDD dioxin receptor by sucrose density gradients demonstrated that the inhibition of Cyp1a gene transcription and DNA binding by TPA resulted from a reduction in nuclear dioxin receptor concentration...
  64. ncbi MEK2 is a kinase related to MEK1 and is differentially expressed in murine tissues
    B K Brott
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell Growth Differ 4:921-9. 1993
    ..Recombinant MEK2 produced in bacteria phosphorylates a kinase-inactive Erk-1 on tyrosine and threonine, whereas a kinase-inactive mutant MEK2 does not. These findings suggest that MEK2 is a member of a multigene family...
  65. ncbi Chromosomal location of three unconventional myosin heavy chain genes in the mouse
    J D Whitmer
    McLaughlin Research Institute, Great Falls, Montana 59405, USA
    Genomics 38:235-7. 1996
    ..The mouse loci have been named Myo1e, Myo1c, and Myo9b; they map to chromosomes 9 (MGD position 41), 11 (MGD position 46), and 8 (MGD position 31.5), respectively...
  66. ncbi Activation of Cyp1a1 and Cyp1a2 genes in adult mouse hepatocytes in primary culture
    N Nemoto
    Department of Experimental Pathology, Cancer Institute, Tokyo
    Jpn J Cancer Res 84:272-8. 1993
    Expression of Cyp1a1 and Cyp1a2 genes was investigated in adult C57BL/6NCrj mouse hepatocytes in primary culture for up to 5 days...
  67. ncbi Chromosomal localization and genomic cloning of the mouse alpha-tropomyosin gene Tpm-1
    M Schleef
    Developmental Biology Unit, University of Bielefeld, Federal Republic of Germany
    Genomics 17:519-21. 1993
    ..Using interspecies backcrosses, we have localized Tpm-1 on mouse chromosome 9, cen-Cyp1a2-Tpm-1-Mod-1-Mylc-Scn5a, near the d-se region...
  68. ncbi Human and mouse mitochondrial orthologs of bacterial ClpX
    T J Corydon
    Institute of Human Genetics, Aarhus University, 8000 Arhus C, Denmark
    Mamm Genome 11:899-905. 2000
    ..Notably, a C-4 zinc finger type motif is recognized in human and mouse ClpX. This motif of so far unknown function is present only in a subset of the known ClpX sequences...
  69. ncbi Conditional disruption of the aryl hydrocarbon receptor nuclear translocator (Arnt) gene leads to loss of target gene induction by the aryl hydrocarbon receptor and hypoxia-inducible factor 1alpha
    S Tomita
    Laboratory of Metabolism, National Cancer Institute, Bethesda, Maryland 20892, USA
    Mol Endocrinol 14:1674-81. 2000
    ..Induction of CYP1A1, CYP1A2, and UGT1*06 mRNAs by the AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin was absent in livers of Arnt-..
  70. ncbi Comparison of mouse hepatic mitochondrial versus microsomal cytochromes P450 following TCDD treatment
    Mary Beth Genter
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, P O Box 670056, Cincinnati, OH 45267 0056, USA
    Biochem Biophys Res Commun 342:1375-81. 2006
    TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) induces cytochromes P450 (CYPs) such as CYP1A1 and CYP1A2 via activation of the aromatic hydrocarbon receptor (AHR)...
  71. ncbi An intron capture strategy used to identify and map a lysyl oxidase-like gene on chromosome 9 in the mouse
    K S Wydner
    Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855 1059, USA
    Genomics 40:342-5. 1997
    ....
  72. ncbi Developmental and tissue-specific differential regulation of the mouse dioxin-inducible P1-450 and P3-450 genes
    N Tuteja
    Dev Biol 112:177-84. 1985
    ....

Research Grants53

  1. RECEPTOR MEDIATED OXIDATIVE STRESS
    HOWARD SHERTZER; Fiscal Year: 2003
    ..2. To determine the mechanism for the TCDD-mediated oxidative stress response in mice, using Ahr, Cyp1a1 and cyp1a2 knockout mice. 3...
  2. Glutathione in Environmental Toxicity and Disease
    HOWARD SHERTZER; Fiscal Year: 2007
    ..Our proposed research should therefore help focus future genotype-phenotype association studies between the appropriate DNA variant sites in the GCLC and GCLM genes and disease. ..
  3. CYP1A1 GENE AND ENVIRONMENTAL TOXICITY
    Daniel Nebert; Fiscal Year: 2005
    The long-term goal of this project is to understand the role of cytochrome P450 1A1 (CYP1A1) in toxicity caused by environmental pollutants...
  4. Sphingolipid signaling in olfactory mucosa
    Mary Beth Genter; Fiscal Year: 2004
    ..abstract_text> ..
  5. Extracellular matrix in chloracetanilide carcinogenesis
    Mary Beth Genter; Fiscal Year: 2004
    ..abstract_text> ..
  6. MOLECULAR GENETICS OF CD TOXICITY
    Daniel Nebert; Fiscal Year: 2004
    ..These studies will enhance our understanding of heavy metal toxicity by identifying and characterizing, for the first time, a major gene responsible for susceptibility to cadmium-induced toxicity. ..
  7. Molecular Genetics of Cadmium Toxicity
    Daniel W Nebert; Fiscal Year: 2010
    ....
  8. PAHs: Balance of Detoxication vs Metabolic Activation
    Daniel Nebert; Fiscal Year: 2009
    ..g. grilled foods & cigarette smoke. Cytochromes P450 1A1 & 1B1 (CYP1A1, CYP1B1) are responsible for the metabolism of numerous PAHs, the prototype of which is benzo[a]pyrene (BaP)...
  9. Molecular Genetics of Cadmium Toxicity
    Daniel Nebert; Fiscal Year: 2009
    ....
  10. PAHs: Balance of Detoxication vs Metabolic Activation
    Daniel Nebert; Fiscal Year: 2009
    ..g. grilled foods & cigarette smoke. Cytochromes P450 1A1 & 1B1 (CYP1A1, CYP1B1) are responsible for the metabolism of numerous PAHs, the prototype of which is benzo[a]pyrene (BaP)...
  11. Molecular Genetics of Cadmium Toxicity
    Daniel Nebert; Fiscal Year: 2007
    ....
  12. Human HNSCC: CYP1B1/1A1/1A2 and AHR Gene Polymorphisms
    Daniel Nebert; Fiscal Year: 2007
    Cytochromes P450 1B1, 1A1 & 1A2 (CYP1B1, CYP1A1, and CYP1A2) are responsible for both detoxifying and metabolically activating innumerable polycyclic aromatic hydrocarbons (PAHs), nitrosamines, and A/-heterocyclics present in ..
  13. CYPIAI GENE AND ENVIRONMENTAL Toxicity
    Daniel Nebert; Fiscal Year: 2003
    ..dioxin in various, tissues (bone marrow, liver, developing embryo) of the Cyp1a1(-/-) knockout and Cyp1a1(+/+) wild-type mouse as a function of dose of the environmental pollutant and route of ..
  14. GENOTOXICITY IN CYP1A2-DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 1993
    The long-term goal of this project is to understand the role that the CYP1A2 (cytochrome P3450) enzyme plays in genotoxicity caused by environmental pollutants in the intact mouse...
  15. CYP1A1 GENE AND ENVIRONMENTAL TOXICITY
    Daniel Nebert; Fiscal Year: 1999
    ..b>Cyp1a1 is a member of the dioxin-inducible [ah] battery, the genes of which are up-regulated by the ah receptor (AHR)...
  16. MECHANISM OF INDUCTION OF OLFACTORY TUMORS BY ALACHLOR
    Mary Beth Genter; Fiscal Year: 2002
    ....
  17. GENOTOXICITY IN CYP1A2 DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 2002
    ..from the Investigator's Abstract) The long-term goal of this project is the elucidation of cytochrome P450 CYP1A2's role in toxicity and cancer caused by environmentally hazardous chemicals...