Cyp19a1

Summary

Gene Symbol: Cyp19a1
Description: cytochrome P450, family 19, subfamily a, polypeptide 1
Alias: ArKO, Cyp19, Int-5, Int5, p450arom, aromatase, CYPXIX, cytochrome P-450AROM, cytochrome P450 19A1, cytochrome P450, 19, aromatase, estrogen synthase
Species: mouse
Products:     Cyp19a1

Top Publications

  1. Murata Y, Robertson K, Jones M, Simpson E. Effect of estrogen deficiency in the male: the ArKO mouse model. Mol Cell Endocrinol. 2002;193:7-12 pubmed
    b>Aromatase, the enzyme responsible for the conversion of androgens to estrogens, is present in the mouse gonads, brain, adipose tissue and bone...
  2. Toda K, Takeda K, Okada T, Akira S, Saibara T, Kaname T, et al. Targeted disruption of the aromatase P450 gene (Cyp19) in mice and their ovarian and uterine responses to 17beta-oestradiol. J Endocrinol. 2001;170:99-111 pubmed
    b>Aromatase P450 (CYP19) is an enzyme catalysing the conversion of androgens into oestrogens...
  3. Takeda K, Toda K, Saibara T, Nakagawa M, Saika K, Onishi T, et al. Progressive development of insulin resistance phenotype in male mice with complete aromatase (CYP19) deficiency. J Endocrinol. 2003;176:237-46 pubmed
    b>Aromatase (CYP19) is a cytochrome P450 enzyme that catalyzes the formation of aromatic C18 estrogens from C19 androgens. It is expressed in various tissues and contributes to sex-specific differences in cellular metabolism...
  4. Chow J, Simpson E, Boon W. Alternative 5'-untranslated first exons of the mouse Cyp19A1 (aromatase) gene. J Steroid Biochem Mol Biol. 2009;115:115-25 pubmed publisher
    The human aromatase gene (CYP19A1) has eleven tissue-specific untranslated first exons, while only three have been described in the mouse Cyp19A1 namely brain-, ovary- and testis-specific exons 1...
  5. Robertson K, Simpson E, Lacham Kaplan O, Jones M. Characterization of the fertility of male aromatase knockout mice. J Androl. 2001;22:825-30 pubmed
    Previous studies employing the male aromatase knockout (ArKO) mouse have indicated that local expression of estrogens appears to be important for the progression of spermatogenesis...
  6. Golovine K, Schwerin M, Vanselow J. Three different promoters control expression of the aromatase cytochrome p450 gene (cyp19) in mouse gonads and brain. Biol Reprod. 2003;68:978-84 pubmed
    b>Aromatase cytochrome P450, the key enzyme of estrogen biosynthesis, is encoded by Cyp19...
  7. Jones M, Thorburn A, Britt K, Hewitt K, Misso M, Wreford N, et al. Aromatase-deficient (ArKO) mice accumulate excess adipose tissue. J Steroid Biochem Mol Biol. 2001;79:3-9 pubmed
    b>Aromatase is the enzyme which catalyses the conversion of C19 steroids into C18 estrogens...
  8. Wu M, Manoli D, Fraser E, Coats J, Tollkuhn J, Honda S, et al. Estrogen masculinizes neural pathways and sex-specific behaviors. Cell. 2009;139:61-72 pubmed publisher
    ..testosterone activates the androgen receptor (AR) and is also converted into estrogen in the brain via aromatase. We demonstrate extensive sexual dimorphism in the number and projections of aromatase-expressing neurons...
  9. Fisher C, Graves K, Parlow A, Simpson E. Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene. Proc Natl Acad Sci U S A. 1998;95:6965-70 pubmed
    The formation of estrogens from C19 steroids is catalyzed by aromatase cytochrome P450 (P450arom), the product of the cyp19 gene...
  10. Juntti S, Tollkuhn J, Wu M, Fraser E, Soderborg T, Tan S, et al. The androgen receptor governs the execution, but not programming, of male sexual and territorial behaviors. Neuron. 2010;66:260-72 pubmed publisher

Detail Information

Publications95

  1. Murata Y, Robertson K, Jones M, Simpson E. Effect of estrogen deficiency in the male: the ArKO mouse model. Mol Cell Endocrinol. 2002;193:7-12 pubmed
    b>Aromatase, the enzyme responsible for the conversion of androgens to estrogens, is present in the mouse gonads, brain, adipose tissue and bone...
  2. Toda K, Takeda K, Okada T, Akira S, Saibara T, Kaname T, et al. Targeted disruption of the aromatase P450 gene (Cyp19) in mice and their ovarian and uterine responses to 17beta-oestradiol. J Endocrinol. 2001;170:99-111 pubmed
    b>Aromatase P450 (CYP19) is an enzyme catalysing the conversion of androgens into oestrogens...
  3. Takeda K, Toda K, Saibara T, Nakagawa M, Saika K, Onishi T, et al. Progressive development of insulin resistance phenotype in male mice with complete aromatase (CYP19) deficiency. J Endocrinol. 2003;176:237-46 pubmed
    b>Aromatase (CYP19) is a cytochrome P450 enzyme that catalyzes the formation of aromatic C18 estrogens from C19 androgens. It is expressed in various tissues and contributes to sex-specific differences in cellular metabolism...
  4. Chow J, Simpson E, Boon W. Alternative 5'-untranslated first exons of the mouse Cyp19A1 (aromatase) gene. J Steroid Biochem Mol Biol. 2009;115:115-25 pubmed publisher
    The human aromatase gene (CYP19A1) has eleven tissue-specific untranslated first exons, while only three have been described in the mouse Cyp19A1 namely brain-, ovary- and testis-specific exons 1...
  5. Robertson K, Simpson E, Lacham Kaplan O, Jones M. Characterization of the fertility of male aromatase knockout mice. J Androl. 2001;22:825-30 pubmed
    Previous studies employing the male aromatase knockout (ArKO) mouse have indicated that local expression of estrogens appears to be important for the progression of spermatogenesis...
  6. Golovine K, Schwerin M, Vanselow J. Three different promoters control expression of the aromatase cytochrome p450 gene (cyp19) in mouse gonads and brain. Biol Reprod. 2003;68:978-84 pubmed
    b>Aromatase cytochrome P450, the key enzyme of estrogen biosynthesis, is encoded by Cyp19...
  7. Jones M, Thorburn A, Britt K, Hewitt K, Misso M, Wreford N, et al. Aromatase-deficient (ArKO) mice accumulate excess adipose tissue. J Steroid Biochem Mol Biol. 2001;79:3-9 pubmed
    b>Aromatase is the enzyme which catalyses the conversion of C19 steroids into C18 estrogens...
  8. Wu M, Manoli D, Fraser E, Coats J, Tollkuhn J, Honda S, et al. Estrogen masculinizes neural pathways and sex-specific behaviors. Cell. 2009;139:61-72 pubmed publisher
    ..testosterone activates the androgen receptor (AR) and is also converted into estrogen in the brain via aromatase. We demonstrate extensive sexual dimorphism in the number and projections of aromatase-expressing neurons...
  9. Fisher C, Graves K, Parlow A, Simpson E. Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene. Proc Natl Acad Sci U S A. 1998;95:6965-70 pubmed
    The formation of estrogens from C19 steroids is catalyzed by aromatase cytochrome P450 (P450arom), the product of the cyp19 gene...
  10. Juntti S, Tollkuhn J, Wu M, Fraser E, Soderborg T, Tan S, et al. The androgen receptor governs the execution, but not programming, of male sexual and territorial behaviors. Neuron. 2010;66:260-72 pubmed publisher
  11. Le May C, Chu K, Hu M, Ortega C, Simpson E, Korach K, et al. Estrogens protect pancreatic beta-cells from apoptosis and prevent insulin-deficient diabetes mellitus in mice. Proc Natl Acad Sci U S A. 2006;103:9232-7 pubmed
    ..we have used mice that were rendered estradiol-deficient or estradiol-resistant by targeted disruption of aromatase (ArKO) or ERalpha (alphaERKO) respectively...
  12. Lubahn D, Moyer J, Golding T, Couse J, Korach K, Smithies O. Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene. Proc Natl Acad Sci U S A. 1993;90:11162-6 pubmed
    ..Prenatal male and female reproductive tract development can therefore occur in the absence of estradiol receptor-mediated responsiveness. ..
  13. Bakker J, Baillien M, Honda S, Harada N, Balthazart J. Relationships between aromatase activity in the brain and gonads and behavioural deficits in homozygous and heterozygous aromatase knockout mice. J Neuroendocrinol. 2004;16:483-90 pubmed
    The present study was carried out to determine whether aromatase knockout (ArKO) mice are completely devoid of aromatase activity in their brain and gonads and to compare aromatase activity in wild-type and ArKO mice, as well as in ..
  14. Naftolin F, Ryan K, Davies I, Reddy V, Flores F, Petro Z, et al. The formation of estrogens by central neuroendocrine tissues. Recent Prog Horm Res. 1975;31:295-319 pubmed
  15. Hewitt K, Pratis K, Jones M, Simpson E. Estrogen replacement reverses the hepatic steatosis phenotype in the male aromatase knockout mouse. Endocrinology. 2004;145:1842-8 pubmed
    The aromatase knockout (ArKO) mouse cannot synthesize endogenous estrogens due to a disruption to the Cyp19 gene...
  16. Honda S, Harada N, Takagi Y. The alternative exons 1 of the mouse aromatase cytochrome P-450 gene. Biochim Biophys Acta. 1996;1305:145-50 pubmed
    b>Aromatase cDNA clones were isolated from cDNA libraries of mouse hypothalamus, amygdala and ovary...
  17. Karolczak M, Kuppers E, Beyer C. Developmental expression and regulation of aromatase- and 5alpha-reductase type I mRNA in the male and female mouse hypothalamus. J Neuroendocrinol. 1998;10:267-74 pubmed
    Androgen metabolites synthesized by neural aromatase and 5alpha-reductase are implicated in many aspects of mammalian brain development and, in particular, in the masculinization of distinct central nervous system structures and brain ..
  18. Brock O, Bakker J. The two kisspeptin neuronal populations are differentially organized and activated by estradiol in mice. Endocrinology. 2013;154:2739-49 pubmed publisher
    ..By using wild-type (WT) and aromatase knockout (ArKO) mice (which cannot convert testosterone into estradiol) and immunohistochemistry, we observed ..
  19. Robertson K, O DONNELL L, Jones M, Meachem S, Boon W, Fisher C, et al. Impairment of spermatogenesis in mice lacking a functional aromatase (cyp 19) gene. Proc Natl Acad Sci U S A. 1999;96:7986-91 pubmed
    ..A., Korach, K. S., and Lubahn, D. B. (1997) Nature (London) 390, 509-512]. Despite the demonstration of the aromatase enzyme, which converts androgens to estrogens, and estrogen receptors within the rodent seminiferous epithelium, ..
  20. Agarwal V, Sinton C, Liang C, Fisher C, German D, Simpson E. Upregulation of estrogen receptors in the forebrain of aromatase knockout (ArKO) mice. Mol Cell Endocrinol. 2000;162:9-16 pubmed
    ..in which estrogen synthesis is completely eliminated by homologous recombination to delete the gene encoding aromatase cytochrome P450 (P450(arom))...
  21. Nemoto Y, Toda K, Ono M, Fujikawa Adachi K, Saibara T, Onishi S, et al. Altered expression of fatty acid-metabolizing enzymes in aromatase-deficient mice. J Clin Invest. 2000;105:1819-25 pubmed
    ..In addition, hepatic steatosis became evident spontaneously in the aromatase-deficient (ArKO) mouse, which lacks intrinsic estrogen production...
  22. Veyrac A, Bakker J. Postnatal and adult exposure to estradiol differentially influences adult neurogenesis in the main and accessory olfactory bulb of female mice. FASEB J. 2011;25:1048-57 pubmed publisher
    ..Taking advantage of female aromatase-knockout (ArKO) mice, which are unable to produce estradiol across their life span, we investigated the role of ..
  23. Li X, MAKELA S, Streng T, Santti R, Poutanen M. Phenotype characteristics of transgenic male mice expressing human aromatase under ubiquitin C promoter. J Steroid Biochem Mol Biol. 2003;86:469-76 pubmed
    ..concentration for the male reproductive functions, we have generated transgenic mice expressing human P450 aromatase under a promoter providing ubiquitous and permanent transgene expression (AROM+ mice)...
  24. Komada M, Takahashi M, Ikeda Y. Involvement of SF-1 in neurogenesis and neuronal migration in the developing neocortex. Neurosci Lett. 2015;600:85-90 pubmed publisher
    ..The mRNA expression of the estrogen receptor ESRα was up-regulated and that of the estrogen synthetase Cyp19a1 was down-regulated in the dorsal telencephalon of SF-1 KO embryos...
  25. Burns K, Yan C, Kumar T, Matzuk M. Analysis of ovarian gene expression in follicle-stimulating hormone beta knockout mice. Endocrinology. 2001;142:2742-51 pubmed
    ..2) Granulosa cells in FSH-deficient mice demonstrate an increase in FSH receptor mRNA, and decreases in P450 aromatase, serum/glucocorticoid-induced kinase, and inhibin/activin subunit mRNAs...
  26. Youngblood G, Nesbitt M, Payne A. The structural genes encoding P450scc and P450arom are closely linked on mouse chromosome 9. Endocrinology. 1989;125:2784-6 pubmed
    ..the two genes that encode the cytochrome P450 enzymes, P450SCC (cholesterol side-chain cleavage) and P450arom (aromatase), was identified in the mouse...
  27. Fang Z, Yang S, Gurates B, Tamura M, Simpson E, Evans D, et al. Genetic or enzymatic disruption of aromatase inhibits the growth of ectopic uterine tissue. J Clin Endocrinol Metab. 2002;87:3460-6 pubmed
    b>Aromatase P450 (P450arom) is the key enzyme for the biosynthesis of estrogen that is essential for the growth of human endometriosis, a pathology characterized by endometrium-like tissue on the peritoneal surfaces of abdominal organs ..
  28. Lee C, Taketo T. Normal onset, but prolonged expression, of Sry gene in the B6.YDOM sex-reversed mouse gonad. Dev Biol. 1994;165:442-52 pubmed
    ..YDOM ovotestis and completely absent in the B6.YDOM ovary. P450 aromatase (P450arom), which converts androgens to estrogens, was transcribed in both control XX and XY gonads at the onset ..
  29. Nitta H, Bunick D, Hess R, Janulis L, Newton S, Millette C, et al. Germ cells of the mouse testis express P450 aromatase. Endocrinology. 1993;132:1396-401 pubmed
    ..Therefore, we have conducted experiments to localize aromatase, a cytochrome P450 enzyme that converts androgen to estrogen, within the testis...
  30. Zhao H, Zhou L, Shangguan A, Bulun S. Aromatase expression and regulation in breast and endometrial cancer. J Mol Endocrinol. 2016;57:R19-33 pubmed publisher
    ..modified mouse models have been generated to help understand the physiological and pathophysiological roles of aromatase and estrogen in the normal breast and in the development of breast cancers...
  31. Li X, Strauss L, MAKELA S, Streng T, Huhtaniemi I, Santti R, et al. Multiple structural and functional abnormalities in the p450 aromatase expressing transgenic male mice are ameliorated by a p450 aromatase inhibitor. Am J Pathol. 2004;164:1039-48 pubmed
    The present study was undertaken to analyze the effect of a P450 aromatase inhibitor (finrozole) on 4-month-old transgenic mice expressing human P450 aromatase (P450arom) under the human ubiquitin C promoter (AROM+)...
  32. Taziaux M, Keller M, Bakker J, Balthazart J. Sexual behavior activity tracks rapid changes in brain estrogen concentrations. J Neurosci. 2007;27:6563-72 pubmed
    ..Brain estrogen production can be regulated within minutes by changes in aromatase (estrogen synthase) activity as a result of calcium-dependent phosphorylations of the enzyme...
  33. Rikke B, Yerg J, Battaglia M, Nagy T, Allison D, Johnson T. Quantitative trait Loci specifying the response of body temperature to dietary restriction. J Gerontol A Biol Sci Med Sci. 2004;59:118-25 pubmed
    ..Importantly, the genes underlying such QTLs would be causal factors affecting these responses and could be identified by positional cloning. ..
  34. Asselin Labat M, Vaillant F, Sheridan J, Pal B, Wu D, Simpson E, et al. Control of mammary stem cell function by steroid hormone signalling. Nature. 2010;465:798-802 pubmed publisher
    ..Notably, even three weeks of treatment with the aromatase inhibitor letrozole was sufficient to reduce the MaSC pool...
  35. Gray D, McGrath C, Jones R, Morris V. A common mouse mammary tumor virus integration site in chemically induced precancerous mammary hyperplasias. Virology. 1986;148:360-8 pubmed
    ..We propose that mouse mammary tumor virus integration at this site is responsible for a specific abnormality in differentiation associated with the preneoplastic phenotype. ..
  36. Simpson E, Jones M, Misso M, Hewitt K, Hill R, Maffei L, et al. Estrogen, a fundamental player in energy homeostasis. J Steroid Biochem Mol Biol. 2005;95:3-8 pubmed
  37. McDaniel A, Li X, Tordoff M, Bachmanov A, Reed D. A locus on mouse Chromosome 9 (Adip5) affects the relative weight of the gonadal but not retroperitoneal adipose depot. Mamm Genome. 2006;17:1078-92 pubmed
    ..within the confidence interval with functional significance to the body fatness phenotype (Il18, Acat1, Cyp19a1, Crabp1, Man2c1, Neil1, Mpi1, Csk, Lsm16, Adpgk, Bbs4, Hexa, Thsd4, Dpp8, Anxa2, and Lipc)...
  38. Tsukahara K, Kakuo S, Moriwaki S, Hotta M, Ohuchi A, Kitahara T, et al. The characteristics of aromatase deficient hairless mice indicate important roles of extragonadal estrogen in the skin. J Steroid Biochem Mol Biol. 2008;108:82-90 pubmed
    ..We examined the skin phenotypes of aromatase-knockout hairless (ArKO) mice and wild-type hairless (WT) mice, both of which were obtained through ..
  39. Barros R, Machado U, Warner M, Gustafsson J. Muscle GLUT4 regulation by estrogen receptors ERbeta and ERalpha. Proc Natl Acad Sci U S A. 2006;103:1605-8 pubmed
    ..in the nuclei of most muscle cells, and that their levels were not affected by absence of estradiol [in aromatase-knockout (ArKO) mice]...
  40. Cisternas C, Tome K, Caeiro X, Dadam F, Garcia Segura L, Cambiasso M. Sex chromosome complement determines sex differences in aromatase expression and regulation in the stria terminalis and anterior amygdala of the developing mouse brain. Mol Cell Endocrinol. 2015;414:99-110 pubmed publisher
    b>Aromatase, which converts testosterone in estradiol, is involved in the generation of brain sex dimorphisms...
  41. Harada N, Yamada K. Ontogeny of aromatase messenger ribonucleic acid in mouse brain: fluorometrical quantitation by polymerase chain reaction. Endocrinology. 1992;131:2306-12 pubmed
    A sensitive method was developed for the determination of trace amounts of aromatase mRNA in various tissues...
  42. Beyer C, Green S, Barker P, Huskisson N, Hutchison J. Aromatase-immunoreactivity is localised specifically in neurones in the developing mouse hypothalamus and cortex. Brain Res. 1994;638:203-10 pubmed
    Local formation of oestrogens from androgens by aromatase cytochrome P-450 within brain cells is crucial for the sexual differentiation of the mammalian CNS...
  43. Toda K, Ono M, Yuhki K, Ushikubi F, Saibara T. 17?-Estradiol is critical for the preovulatory induction of prostaglandin E(2) synthesis in mice. Mol Cell Endocrinol. 2012;362:176-82 pubmed publisher
    b>Aromatase-deficient (ArKO) mice are totally anovulatory due to insufficient estrogen production. However, sequential administrations of high doses of 17?-estradiol (E2) and gonadotropins were found to induce ovulation in these mice...
  44. Bertrand P, Paranavitane U, Chavez C, Gogos A, Jones M, van den Buuse M. The effect of low estrogen state on serotonin transporter function in mouse hippocampus: a behavioral and electrochemical study. Brain Res. 2005;1064:10-20 pubmed
    ..We compared control mice, ovariectomized (OVX) C57BL/6J mice and aromatase knockout (ArKO) mice that are unable to produce estrogen...
  45. Johnston W, Salmon M, Su G, Lu G, Ailawadi G, Upchurch G. Aromatase is required for female abdominal aortic aneurysm protection. J Vasc Surg. 2015;61:1565-74.e1-4 pubmed publisher
    ..Estrogen synthesis is dependent on the enzyme aromatase, which is located both centrally in the ovaries and peripherally in adipose tissue, bone, and vascular smooth ..
  46. Liljander M, Andersson A, Holmdahl R, Mattsson R. Increased litter size and super-ovulation rate in congenic C57BL mice carrying a polymorphic fragment of NFR/N origin at the Fecq4 locus of chromosome 9. Genet Res (Camb). 2009;91:259-65 pubmed publisher
    ..Genes of the cytochrome P450 family (1, 11 and 19), such as Cyp19a1, are assumed to be particularly interesting, since they are known to exhibit female-associated reproductive ..
  47. Honda S, Matsumoto T, Harada N. Characterization and purification of a protein binding to the cis-acting element for brain-specific exon 1 of the mouse aromatase gene. J Steroid Biochem Mol Biol. 2001;79:255-60 pubmed
    ..Androgen exerts its action on the brain after conversion to estrogen by brain aromatase. The aromatase appears in some neural nuclei such as in the hypothalamus and amygdala, and has been indicated to ..
  48. Kimura M, Sudhir K, Jones M, Simpson E, Jefferis A, Chin Dusting J. Impaired acetylcholine-induced release of nitric oxide in the aorta of male aromatase-knockout mice: regulation of nitric oxide production by endogenous sex hormones in males. Circ Res. 2003;93:1267-71 pubmed
    ..The effects of endogenous estrogen deficiency in males are less clear. The aromatase-knockout (ArKO) mouse lacks a functional Cyp19 gene, which encodes aromatase, and is thus incapable of ..
  49. Wozniak A, Hutchison R, Hutchison J. Localisation of aromatase activity in androgen target areas of the mouse brain. Neurosci Lett. 1992;146:191-4 pubmed
    Oestrogens are formed from androgens in the mouse brain, but the localization of aromatase activity is unresolved. Immunocytochemistry has not detected aromatase in expected key androgen target areas such as the medial preoptic area...
  50. Boulanger G, Cibois M, Viet J, Fostier A, Deschamps S, Pastezeur S, et al. Hypogonadism Associated with Cyp19a1 (Aromatase) Posttranscriptional Upregulation in Celf1 Knockout Mice. Mol Cell Biol. 2015;35:3244-53 pubmed publisher
    ..Cyp19a1 encodes aromatase, which transforms testosterone into estradiol...
  51. Shen J, Liu J, Xie Y, Diwan B, Waalkes M. Fetal onset of aberrant gene expression relevant to pulmonary carcinogenesis in lung adenocarcinoma development induced by in utero arsenic exposure. Toxicol Sci. 2007;95:313-20 pubmed
    ..factor-3, anterior gradient-2, and the steroid metabolism genes 17-beta-hydroxysteroid dehydrogenase type 5 and aromatase. The insulin growth factor system, which can be influenced by ER and has been implicated in the pulmonary ..
  52. Robertson K, O Donnell L, Simpson E, Jones M. The phenotype of the aromatase knockout mouse reveals dietary phytoestrogens impact significantly on testis function. Endocrinology. 2002;143:2913-21 pubmed
    ..cells and seminiferous epithelium, and its importance in spermatogenesis is highlighted by the phenotype of the aromatase knockout (ArKO) mouse. These mice are unable to synthesize endogenous estrogens...
  53. Honda S, Harada N, Abe Dohmae S, Takagi Y. Identification of cis-acting elements in the proximal promoter region for brain-specific exon 1 of the mouse aromatase gene. Brain Res Mol Brain Res. 1999;66:122-32 pubmed
    Among multiple exons 1 of the mouse aromatase gene, brain-specific exon 1 is only utilized in the hypothalamus and amygdala regions...
  54. Britt K, Drummond A, Cox V, Dyson M, Wreford N, Jones M, et al. An age-related ovarian phenotype in mice with targeted disruption of the Cyp 19 (aromatase) gene. Endocrinology. 2000;141:2614-23 pubmed
    With the development of a mouse model of estrogen insufficiency due to targeted disruption of the aromatase gene [the aromatase knockout (ArKO) mouse], a new opportunity exists to examine the role of estrogen in ovarian follicular ..
  55. Toda K, Saibara T, Okada T, Onishi S, Shizuta Y. A loss of aggressive behaviour and its reinstatement by oestrogen in mice lacking the aromatase gene (Cyp19). J Endocrinol. 2001;168:217-20 pubmed
    b>Aromatase P450 (CYP19) is an enzyme responsible for conversion of androgens to oestrogens...
  56. Delarue B, Mittre H, Feral C, Benhaim A, Leymarie P. Rapid sequencing of rabbit aromatase cDNA using RACE PCR. C R Acad Sci III. 1996;319:663-70 pubmed
    The sequencing of aromatase cDNA from rabbit granulosa cells was obtained by RACE PCR. This cDNA is 2.9 kb long. The first 119 nucleotides correspond to the first untranslated exon...
  57. Keshava N, Mandava U, Kirma N, Tekmal R. Acceleration of mammary neoplasia in aromatase transgenic mice by 7,12-dimethylbenz[a]anthracene. Cancer Lett. 2001;167:125-33 pubmed
    Our studies using the aromatase transgenic mice model have shown that early exposure of mammary epithelium to in situ estrogen as a result of overexpression of aromatase predispose mammary tissue to preneoplastic changes...
  58. Tekmal R, Liu Y, Nair H, Jones J, Perla R, Lubahn D, et al. Estrogen receptor alpha is required for mammary development and the induction of mammary hyperplasia and epigenetic alterations in the aromatase transgenic mice. J Steroid Biochem Mol Biol. 2005;95:9-15 pubmed
    b>Aromatase transgenic mice exhibit hyperplastic and dysplastic changes, attesting to the importance of local estrogen in breast carcinogenesis...
  59. Tekmal R, Nair H, Perla R, Kirma N. HER-2/neu x aromatase double transgenic mice model: the effects of aromatase overexpression on mammary tumorigenesis. J Steroid Biochem Mol Biol. 2007;106:111-8 pubmed
    ..To address the importance of local estrogen in oncogene-mediated breast tumorigenesis, we have crossed MMTV-aromatase with MMTV-HER2/neu and examined the incidence of breast cancer in double transgenic mice in comparison with ..
  60. Pierman S, Sica M, Allieri F, Viglietti Panzica C, Panzica G, Bakker J. Activational effects of estradiol and dihydrotestosterone on social recognition and the arginine-vasopressin immunoreactive system in male mice lacking a functional aromatase gene. Horm Behav. 2008;54:98-106 pubmed publisher
    ..We demonstrate here that gonadally intact male aromatase knockout (ArKO) mice, which cannot aromatize androgens into estrogens due to a targeted mutation in the ..
  61. Toyooka Y, Tanaka S, Hirota O, Tanaka S, Takagi N, Yamanouchi K, et al. Wilms' tumor suppressor gene (WT1) as a target gene of SRY function in a mouse ES cell line transfected with SRY. Int J Dev Biol. 1998;42:1143-51 pubmed
    ..Among the five potential SRY-target genes examined, i.e., MIS, SF1, P450arom, Sox9 and WT1, only the expression of WT1 was induced de novo by the unscheduled expression of Sry in the ..
  62. Gray S, Mannan M, O Shaughnessy P. Development of cytochrome P450 aromatase mRNA levels and enzyme activity in ovaries of normal and hypogonadal (hpg) mice. J Mol Endocrinol. 1995;14:295-301 pubmed
    The cytochrome P450 aromatase (P450arom) enzyme is required for bioconversion of androgen to oestrogen...
  63. Toda K, Okada T, Takeda K, Akira S, Saibara T, Shiraishi M, et al. Oestrogen at the neonatal stage is critical for the reproductive ability of male mice as revealed by supplementation with 17beta-oestradiol to aromatase gene (Cyp19) knockout mice. J Endocrinol. 2001;168:455-63 pubmed
    b>Aromatase P450 (CYP19) is an enzyme responsible for the conversion of androgens to oestrogens. We generated CYP19 knockout (ArKO) mice by targeted disruption of Cyp19 and studied the role of oestrogens in male reproductive ability...
  64. Bunting M, Bernstein K, Greer J, Capecchi M, Thomas K. Targeting genes for self-excision in the germ line. Genes Dev. 1999;13:1524-8 pubmed
    ..In these chimeras, somatic cells derived from the ES cells retained the cassette, but self-excision occurred in all ES-cell-derived sperm. ..
  65. Rahimi Darabad R, Suzuki T, Richards S, Jakobiec F, Zakka F, Barabino S, et al. Does estrogen deficiency cause lacrimal gland inflammation and aqueous-deficient dry eye in mice?. Exp Eye Res. 2014;127:153-60 pubmed publisher
    ..Lacrimal glands were obtained from adult, age-matched wild type (WT) and aromatase knockout (ArKO) mice, in which estrogen synthesis is completely eliminated...
  66. Plumari L, Viglietti Panzica C, Allieri F, Honda S, Harada N, Absil P, et al. Changes in the arginine-vasopressin immunoreactive systems in male mice lacking a functional aromatase gene. J Neuroendocrinol. 2002;14:971-8 pubmed
    ..We used knockout mice lacking a functional aromatase enzyme (ArKO) to investigate the effects of a chronic depletion of oestrogens on the sexually dimorphic AVP ..
  67. Dalla C, Antoniou K, Papadopoulou Daifoti Z, Balthazart J, Bakker J. Oestrogen-deficient female aromatase knockout (ArKO) mice exhibit depressive-like symptomatology. Eur J Neurosci. 2004;20:217-28 pubmed
    We recently found that female aromatase knockout (ArKO) mice that are deficient in oestradiol due to a targeted mutation in the aromatase gene show deficits in sexual behaviour that cannot be corrected by adult treatment with oestrogens...
  68. Ijiri S, Kazeto Y, Lokman P, Adachi S, Yamauchi K. Characterization of a cDNA encoding P-450 aromatase (CYP19) from Japanese eel ovary and its expression in ovarian follicles during induced ovarian development. Gen Comp Endocrinol. 2003;130:193-203 pubmed
    A cDNA encoding P450 aromatase (CYP19) was isolated from a Japanese eel (Anguilla japonica) ovarian cDNA library. This cDNA contains a complete open reading frame encoding 511 amino acid residues...
  69. Azcoitia I, Sierra A, Veiga S, Garcia Segura L. Aromatase expression by reactive astroglia is neuroprotective. Ann N Y Acad Sci. 2003;1007:298-305 pubmed
    The enzyme aromatase catalyzes the conversion of testosterone and other C19 steroids to estradiol. Under normal circumstances, the expression of aromatase in the central nervous system of mammals is restricted to neurons...
  70. Pierman S, Douhard Q, Balthazart J, Baum M, Bakker J. Attraction thresholds and sex discrimination of urinary odorants in male and female aromatase knockout (ArKO) mice. Horm Behav. 2006;49:96-104 pubmed
    We previously found that both male and female aromatase knockout (ArKO) mice, which cannot synthesize estrogens due to a targeted mutation of the aromatase gene, showed less investigation of volatile body odors from anesthetized ..
  71. Toda K, Hayashi Y, Ono M, Saibara T. Impact of ovarian sex steroids on ovulation and ovulatory gene induction in aromatase-null mice. Endocrinology. 2012;153:386-94 pubmed publisher
    Female mice deficient in the aromatase gene [aromatase knockout (ArKO)] fail to ovulate owing to an inability to produce estrogens...
  72. Hill R, McInnes K, Gong E, Jones M, Simpson E, Boon W. Estrogen deficient male mice develop compulsive behavior. Biol Psychiatry. 2007;61:359-66 pubmed
    b>Aromatase converts androgen to estrogen. Thus, the aromatase knockout (ArKO) mouse is estrogen deficient...
  73. Meltser I, Tahera Y, Simpson E, Hultcrantz M, Charitidi K, Gustafsson J, et al. Estrogen receptor beta protects against acoustic trauma in mice. J Clin Invest. 2008;118:1563-70 pubmed publisher
    ..ERbeta in the auditory system by investigating mice deficient in ERalpha (ERKO mice), ERbeta (BERKO mice), and aromatase (ARKO mice). Basal auditory brainstem response (ABR) thresholds were similar in all animals...
  74. Bakker J, Pierman S, González Martínez D. Effects of aromatase mutation (ArKO) on the sexual differentiation of kisspeptin neuronal numbers and their activation by same versus opposite sex urinary pheromones. Horm Behav. 2010;57:390-5 pubmed publisher
    ..Furthermore, we analyzed the effects of aromatase mutation (ArKO) and thus the role of estradiol on RP3V kisspeptin neuronal numbers and on the response of these ..
  75. Boon W, Diepstraten J, van der Burg J, Jones M, Simpson E, van den Buuse M. Hippocampal NMDA receptor subunit expression and watermaze learning in estrogen deficient female mice. Brain Res Mol Brain Res. 2005;140:127-32 pubmed
    The aromatase knockout (ArKO) mouse is estrogen deficient...
  76. Vyazovskiy V, Kopp C, Wigger E, Jones M, Simpson E, Tobler I. Sleep and rest regulation in young and old oestrogen-deficient female mice. J Neuroendocrinol. 2006;18:567-76 pubmed
    The effect of circulating oestrogen deficiency on sleep regulation and locomotor activity was investigated in aromatase cytochrome P450 deficient mice (ArKO) and wild-type (WT) controls...
  77. Pierman S, Tirelli E, Douhard Q, Baum M, Bakker J. Male aromatase knockout mice acquire a conditioned place preference for cocaine but not for contact with an estrous female. Behav Brain Res. 2006;174:64-9 pubmed
    We have previously shown that male mice carrying a targeted mutation in the Cyp19 gene which encodes the aromatase enzyme (aromatase knockout or ArKO), showed a reduced interest to investigate volatile odors from conspecifics in a Y-maze...
  78. Zhu W, Wang L, Zhang L, Palmateer J, Libal N, Hurn P, et al. Isoflurane preconditioning neuroprotection in experimental focal stroke is androgen-dependent in male mice. Neuroscience. 2010;169:758-69 pubmed publisher
    ..receptor antagonist), or castrated and implanted with testosterone, dihydrotestosterone, flutamide, letrozole (aromatase inhibitor), or vehicle 7-13 days before preconditioning...
  79. McPherson S, Hussain S, Balanathan P, Hedwards S, Niranjan B, Grant M, et al. Estrogen receptor-beta activated apoptosis in benign hyperplasia and cancer of the prostate is androgen independent and TNFalpha mediated. Proc Natl Acad Sci U S A. 2010;107:3123-8 pubmed publisher
    ..apoptosis in prostatic stromal, luminal and castrate-resistant basal epithelial cells of estrogen-deficient aromatase knock-out mice...
  80. Fester L, Labitzke J, Hinz R, Behem C, Horling K, Bernhard T, et al. Estradiol responsiveness of synaptopodin in hippocampal neurons is mediated by estrogen receptor ?. J Steroid Biochem Mol Biol. 2013;138:455-61 pubmed publisher
    ..In this study, we show, unexpectedly, that synaptopodin is downregulated in the hippocampus of aromatase knock-out mice and that inhibition of neuronal estradiol synthesis using the aromatase inhibitor letrozole also ..
  81. Britt K, Kerr J, O Donnell L, Jones M, Drummond A, Davis S, et al. Estrogen regulates development of the somatic cell phenotype in the eutherian ovary. FASEB J. 2002;16:1389-97 pubmed
    ..The gonads of female mice incapable of synthesizing estrogens due to disruption of the aromatase gene (ArKO) provide a unique model to test the role of estrogen in regulating the gonadal phenotype...
  82. Kuntz S, Chesnel A, Duterque Coquillaud M, Grillier Vuissoz I, Callier M, Dournon C, et al. Differential expression of P450 aromatase during gonadal sex differentiation and sex reversal of the newt Pleurodeles waltl. J Steroid Biochem Mol Biol. 2003;84:89-100 pubmed
    ..In the newt Pleurodeles waltl, a P450 aromatase cDNA was isolated from adult gonads, and the nucleotide or deduced amino acid sequences showed a high level of ..