Gene Symbol: Cstf2
Description: cleavage stimulation factor, 3' pre-RNA subunit 2
Alias: C630034J23Rik, Cstf64, mir-3112, cleavage stimulation factor subunit 2, CF-1 64 kDa subunit, CSTF 64 kDa subunit, alphaCstF-64 variant 4, betaCstF-64 variant 1, betaCstF-64 variant 3, cleavage stimulation factor, 3' pre-RNA subunit 2, 64 kDa
Species: mouse
Products:     Cstf2

Top Publications

  1. Martincic K, Campbell R, Edwalds Gilbert G, Souan L, Lotze M, Milcarek C. Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition. Proc Natl Acad Sci U S A. 1998;95:11095-100 pubmed
    ..Because augmentation of CstF-64 levels is neither necessary nor sufficient for Ig secretory mRNA production, we conclude that other lymphokine-induced factors play a role. ..
  2. Wallace A, Denison T, Attaya E, MacDonald C. Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis. Biol Reprod. 2004;70:1080-7 pubmed
    ..In mice, two forms of the regulatory polyadenylation protein CstF-64 are found. The gene Cstf2 on the X chromosome encodes this form, and it is expressed in all somatic tissues...
  3. Takagaki Y, Seipelt R, Peterson M, Manley J. The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation. Cell. 1996;87:941-52 pubmed
    ..Our results indicate that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation. ..
  4. Wallace A, Dass B, Ravnik S, Tonk V, Jenkins N, Gilbert D, et al. Two distinct forms of the 64,000 Mr protein of the cleavage stimulation factor are expressed in mouse male germ cells. Proc Natl Acad Sci U S A. 1999;96:6763-8 pubmed
    ..CstF-64 may, therefore, be absent in spermatocytes because the X chromosome is inactivated during male meiosis. By extension, the testis-specific CstF-64 may be expressed from an autosomal homolog of the X chromosomal gene. ..
  5. Monarez R, MacDonald C, Dass B. Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers. Biochem J. 2007;401:651-8 pubmed
    ..This supports the hypothesis that tauCstF-64 promotes germ-cell-specific patterns of polyadenylation by binding to different downstream sequence elements. ..
  6. Li L, Roy K, Katyal S, Sun X, Bleoo S, Godbout R. Dynamic nature of cleavage bodies and their spatial relationship to DDX1 bodies, Cajal bodies, and gems. Mol Biol Cell. 2006;17:1126-40 pubmed
    ..Our results suggest that cleavage body components are highly dynamic in nature. ..
  7. Shankarling G, Coates P, Dass B, MacDonald C. A family of splice variants of CstF-64 expressed in vertebrate nervous systems. BMC Mol Biol. 2009;10:22 pubmed publisher
    ..We propose that betaCstF-64 contributes to proteomic diversity by regulating alternative polyadenylation of neural mRNAs. ..
  8. Romeo V, Griesbach E, Schümperli D. CstF64: cell cycle regulation and functional role in 3' end processing of replication-dependent histone mRNAs. Mol Cell Biol. 2014;34:4272-84 pubmed publisher
    ..HLF), composed of cleavage/polyadenylation specificity factor, symplekin, and cleavage stimulation factor 64 (CstF64). Although HLF has been shown to be cell cycle regulated, the mechanism of this regulation is unknown...
  9. Dass B, Attaya E, Michelle Wallace A, MacDonald C. Overexpression of the CstF-64 and CPSF-160 polyadenylation protein messenger RNAs in mouse male germ cells. Biol Reprod. 2001;64:1722-9 pubmed
    ..Proc Natl Acad Sci U S A 1999; 96:6763-6768), which suggests that overexpression of mRNA transcription and processing factors plays an essential role in postmeiotic germ cell mRNA metabolism. ..

More Information


  1. Martincic K, Alkan S, Cheatle A, Borghesi L, Milcarek C. Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing. Nat Immunol. 2009;10:1102-9 pubmed publisher
    ..Thus, loading of ELL2 and CstF-64 on RNA polymerase II was linked, caused enhanced use of the proximal poly(A) site and was necessary for processing of immunoglobulin heavy-chain mRNA. ..
  2. Yao C, Choi E, Weng L, Xie X, Wan J, Xing Y, et al. Overlapping and distinct functions of CstF64 and CstF64? in mammalian mRNA 3' processing. RNA. 2013;19:1781-90 pubmed publisher
    ..However, the underlying mechanisms remain poorly understood. CstF64? is a paralog of the general mRNA 3' processing factor, CstF64, and has been implicated in mediating testis-..
  3. Youngblood B, Grozdanov P, MacDonald C. CstF-64 supports pluripotency and regulates cell cycle progression in embryonic stem cells through histone 3' end processing. Nucleic Acids Res. 2014;42:8330-42 pubmed publisher
    ..These results suggest that CstF-64 plays a key role in modulating the cell cycle in ESCs while simultaneously controlling histone mRNA 3' end processing. ..
  4. Youngblood B, MacDonald C. CstF-64 is necessary for endoderm differentiation resulting in cardiomyocyte defects. Stem Cell Res. 2014;13:413-21 pubmed publisher
    ..However, CstF-64 knockout (Cstf2(E6)) cells were able to differentiate into neuronal progenitors, demonstrating that some differentiation pathways ..
  5. Nazim M, Masuda A, Rahman M, Nasrin F, Takeda J, Ohe K, et al. Competitive regulation of alternative splicing and alternative polyadenylation by hnRNP H and CstF64 determines acetylcholinesterase isoforms. Nucleic Acids Res. 2017;45:1455-1468 pubmed publisher
    ..Furthermore, hnRNP H competes for binding of CstF64 to the overlapping binding sites in exon 5a, and suppresses the selection of a cryptic polyadenylation site (PAS), ..