Gene Symbol: Cr1l
Description: complement component (3b/4b) receptor 1-like
Alias: Crry, Mcp, mCRY, complement component receptor 1-like protein, complement receptor related protein, complement regulatory protein Crry, protein p65
Species: mouse
Products:     Cr1l

Top Publications

  1. Fernández Centeno E, de Ojeda G, Rojo J, Portoles P. Crry/p65, a membrane complement regulatory protein, has costimulatory properties on mouse T cells. J Immunol. 2000;164:4533-42 pubmed
    ..In this study, we describe a novel function for the mouse RCA type I membrane protein Crry/p65 as a costimulatory molecule in CD4+ T cell activation...
  2. Molina H. The murine complement regulator Crry: new insights into the immunobiology of complement regulation. Cell Mol Life Sci. 2002;59:220-9 pubmed
    ..Murine Crry is one of these regulators that inhibits the activation of the third component of complement and protects self-..
  3. Aslam M, Guthridge J, Hack B, Quigg R, Holers V, Perkins S. The extended multidomain solution structures of the complement protein Crry and its chimeric conjugate Crry-Ig by scattering, analytical ultracentrifugation and constrained modelling: implications for function and therapy. J Mol Biol. 2003;329:525-50 pubmed
    Complement receptor-related gene/protein y (Crry) is a cell membrane-bound regulator of complement activation found in mouse and rat. Crry contains only short complement/consensus repeat (SCR) domains...
  4. Miwa T, Zhou L, Tudoran R, Lambris J, Madaio M, Nangaku M, et al. DAF/Crry double deficiency in mice exacerbates nephrotoxic serum-induced proteinuria despite markedly reduced systemic complement activity. Mol Immunol. 2007;44:139-46 pubmed
    Decay-accelerating factor (DAF) and complement receptor 1-related gene/protein y (Crry) are two membrane-anchored complement regulatory proteins in rodent...
  5. Mao D, Wu X, Deppong C, Friend L, Dolecki G, Nelson D, et al. Negligible role of antibodies and C5 in pregnancy loss associated exclusively with C3-dependent mechanisms through complement alternative pathway. Immunity. 2003;19:813-22 pubmed
    ..These findings provide significant insight into the role of the innate immune system in human pregnancy failure, a frequent clinical outcome. ..
  6. Kim Y, Kinoshita T, Molina H, Hourcade D, Seya T, Wagner L, et al. Mouse complement regulatory protein Crry/p65 uses the specific mechanisms of both human decay-accelerating factor and membrane cofactor protein. J Exp Med. 1995;181:151-9 pubmed
    ..Previous studies have shown that the mouse protein Crry/p65 has certain characteristics of self-protecting complement regulatory proteins...
  7. Li B, Sallee C, Dehoff M, Foley S, Molina H, Holers V. Mouse Crry/p65. Characterization of monoclonal antibodies and the tissue distribution of a functional homologue of human MCP and DAF. J Immunol. 1993;151:4295-305 pubmed
    In previous studies we have demonstrated that mouse Crry/p65 regulates complement component C3 deposition on self membranes, a functional property that both human decay-accelerating factor (DAF) and membrane cofactor protein (MCP) exhibit...
  8. Renner B, Coleman K, Goldberg R, Amura C, Holland Neidermyer A, Pierce K, et al. The complement inhibitors Crry and factor H are critical for preventing autologous complement activation on renal tubular epithelial cells. J Immunol. 2010;185:3086-94 pubmed publisher
    ..We found that Crry is the only membrane-bound complement regulator expressed by murine TECs, and its expression is concentrated on the ..
  9. Paul M, Aegerter M, Cepek K, Miller M, Weis J. The murine complement receptor gene family. III. The genomic and transcriptional complexity of the Crry and Crry-ps genes. J Immunol. 1990;144:1988-96 pubmed
    The murine CR genes Crry (previously termed mCRY) and Crry-ps (previously termed mCRX) are two distinct, but related, sequences which are the evolutionary homologs to sequences contained within the human CR1 gene...

More Information


  1. Barata L, Miwa T, Sato S, Kim D, Mohammed I, Song W. Deletion of Crry and DAF on murine platelets stimulates thrombopoiesis and increases factor H-dependent resistance of peripheral platelets to complement attack. J Immunol. 2013;190:2886-95 pubmed publisher
    Complement receptor 1-related gene/protein y (Crry) and decay-accelerating factor (DAF) are two murine membrane C3 complement regulators with overlapping functions...
  2. Ramaglia V, Hughes T, Donev R, Ruseva M, Wu X, Huitinga I, et al. C3-dependent mechanism of microglial priming relevant to multiple sclerosis. Proc Natl Acad Sci U S A. 2012;109:965-70 pubmed publisher
    ..We show that deleting the C3 convertase regulator complement receptor 1-related protein y (Crry) induces microglial priming...
  3. Xu C, Mao D, Holers V, Palanca B, Cheng A, Molina H. A critical role for murine complement regulator crry in fetomaternal tolerance. Science. 2000;287:498-501 pubmed
    ..Mice that were deficient in another murine complement regulator, Crry, were generated to investigate its role in vivo...
  4. Thurman J, Ljubanovic D, Royer P, Kraus D, Molina H, Barry N, et al. Altered renal tubular expression of the complement inhibitor Crry permits complement activation after ischemia/reperfusion. J Clin Invest. 2006;116:357-68 pubmed
    ..We found that loss of polarity of complement receptor 1-related protein y (Crry) in the tubular epithelium preceded activation of the alternative pathway along the basolateral aspect of the ..
  5. Molina H, Wong W, Kinoshita T, Brenner C, Foley S, Holers V. Distinct receptor and regulatory properties of recombinant mouse complement receptor 1 (CR1) and Crry, the two genetic homologues of human CR1. J Exp Med. 1992;175:121-9 pubmed
    ..A five SCR-containing transcript derived from a second unique gene, designated Crry, also crosshybridizes with human CR1...
  6. Bao L, Wang Y, Haas M, Quigg R. Distinct roles for C3a and C5a in complement-induced tubulointerstitial injury. Kidney Int. 2011;80:524-34 pubmed publisher
    ..An influx of circulating C3 from a syngeneic host into donor kidneys deficient in Crry (a membrane protein that reduces C3 convertase activity) causes spontaneous complement activation, primarily in the ..
  7. Miwa T, Zhou L, Kimura Y, Kim D, Bhandoola A, Song W. Complement-dependent T-cell lymphopenia caused by thymocyte deletion of the membrane complement regulator Crry. Blood. 2009;113:2684-94 pubmed publisher
    ..Here, we show that conditional gene targeting of a murine membrane complement regulator Crry on thymocytes led to complement-dependent peripheral T-cell lymphopenia...
  8. Ruseva M, Hughes T, Donev R, Sivasankar B, Pickering M, Wu X, et al. Crry deficiency in complement sufficient mice: C3 consumption occurs without associated renal injury. Mol Immunol. 2009;46:803-11 pubmed publisher
    The rodent-specific complement regulator complement receptor 1-related gene/protein-y (Crry) is critical for complement homeostasis...
  9. Wu X, Spitzer D, Mao D, Peng S, Molina H, Atkinson J. Membrane protein Crry maintains homeostasis of the complement system. J Immunol. 2008;181:2732-40 pubmed
    Complement activation is tightly regulated to avoid excessive inflammatory and immune responses. Crry(-/-) is an embryonic lethal phenotype secondary to the maternal complement alternative pathway (AP) attacking a placenta deficient in ..
  10. Kim D, Miwa T, Song W. Retrovirus-mediated over-expression of decay-accelerating factor rescues Crry-deficient erythrocytes from acute alternative pathway complement attack. J Immunol. 2006;177:5558-66 pubmed
    Decay-accelerating factor (DAF) and complement receptor 1-related gene/protein y (Crry) are two membrane-bound complement regulators on murine erythrocytes that inhibit C3/C5 convertases...
  11. Kingsmore S, Vik D, Kurtz C, Leroy P, Tack B, Weis J, et al. Genetic organization of complement receptor-related genes in the mouse. J Exp Med. 1989;169:1479-84 pubmed
    ..cross, gene linkage relationships among members of the murine complement receptor-related genes, C4bp, Cfh, Mcry, and Mcr2, were analyzed by segregation of RFLP in 200 mice...
  12. Briggs D, Martin C, Ingersoll S, Barnum S, Martin B. Astrocyte-specific expression of a soluble form of the murine complement control protein Crry confers demyelination protection in the cuprizone model. Glia. 2007;55:1405-15 pubmed
    ..Simultaneously, these mice lost expression of the rodent complement regulatory protein, Crry. A soluble CNS-specific form of the Crry protein (sCrry) expressed in a transgenic mouse under the control of an ..
  13. Banda N, Kraus D, Muggli M, Bendele A, Holers V, Arend W. Prevention of collagen-induced arthritis in mice transgenic for the complement inhibitor complement receptor 1-related gene/protein y. J Immunol. 2003;171:2109-15 pubmed
    ..C57BL/6 mice transgenic for the rodent complement regulatory protein complement receptor 1-related gene/protein y (Crry) (Crry-Tg), a C3 convertase inhibitor...
  14. Jiménez Periañez A, Ojeda G, Criado G, Sánchez A, Pini E, Madrenas J, et al. Complement regulatory protein Crry/p65-mediated signaling in T lymphocytes: role of its cytoplasmic domain and partitioning into lipid rafts. J Leukoc Biol. 2005;78:1386-96 pubmed
    b>Crry/p65 is a type I glycoprotein, which protects mouse T cells from complement attack...
  15. Atkinson C, Song H, Lu B, Qiao F, Burns T, Holers V, et al. Targeted complement inhibition by C3d recognition ameliorates tissue injury without apparent increase in susceptibility to infection. J Clin Invest. 2005;115:2444-53 pubmed iC3b/C3dg-binding fragment of mouse complement receptor 2 (CR2) to a mouse complement-inhibitory protein, Crry. We show that the novel CR2-Crry fusion protein targets sites of local and remote (lung) complement activation ..
  16. Kim D, Miwa T, Kimura Y, Schwendener R, van Lookeren Campagne M, Song W. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19 pubmed publisher that deficiency of either decay-accelerating factor (DAF) or complement receptor 1-related gene/protein y (Crry) on murine platelets was inconsequential, whereas DAF and Crry double deficiency led to rapid clearance of ..
  17. Miwa T, Zhou L, Hilliard B, Molina H, Song W. Crry, but not CD59 and DAF, is indispensable for murine erythrocyte protection in vivo from spontaneous complement attack. Blood. 2002;99:3707-16 pubmed
    ..In contrast, mouse erythrocytes deficient in complement receptor 1 (CR1)-related gene y (Crry), a membrane C3 inhibitor with DAF and membrane cofactor protein activities, were rapidly eliminated from the ..
  18. Miao J, Lesher A, Miwa T, Sato S, Gullipalli D, Song W. Tissue-specific deletion of Crry from mouse proximal tubular epithelial cells increases susceptibility to renal ischemia-reperfusion injury. Kidney Int. 2014;86:726-37 pubmed publisher
    The murine cell surface protein Crry (complement receptor 1-related protein/gene y) is a key complement regulator with similar activities to human membrane cofactor protein (MCP) and decay-accelerating factor...
  19. Miwa T, Zhou L, Maldonado M, Madaio M, Eisenberg R, Song W. Absence of CD59 exacerbates systemic autoimmunity in MRL/lpr mice. J Immunol. 2012;189:5434-41 pubmed publisher
    ..These results together suggest that CD59a inhibits systemic autoimmunity in MRL/lpr mice through a complement-independent mechanism. ..
  20. Utani A, Kopp J, Kozak C, Matsuki Y, Amizuka N, Sugiyama S, et al. Mouse kalinin B1 (laminin beta 3 chain): cloning and tissue distribution. Lab Invest. 1995;72:300-10 pubmed
    ..In vitro studies indicate that the beta 3 chain participates in trimer formation with the gamma 2 chain and possibly with other laminin chains as well. ..
  21. Molina H, Miwa T, Zhou L, Hilliard B, Mastellos D, Maldonado M, et al. Complement-mediated clearance of erythrocytes: mechanism and delineation of the regulatory roles of Crry and DAF. Decay-accelerating factor. Blood. 2002;100:4544-9 pubmed
    ..mouse erythrocytes deficient in the membrane C3 regulatory protein, complement receptor 1-related gene/protein y (Crry), but not decay-accelerating factor (DAF), were spontaneously eliminated in vivo by complement...
  22. Kurtz C, O TOOLE E, Christensen S, Weis J. The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1. J Immunol. 1990;144:3581-91 pubmed
    ..DNA sequence analysis, RNA protection studies and genomic phage mapping indicate the transcripts which encode these proteins are derived from the Cr2 gene via alternative splicing. ..
  23. Chaves L, Bao L, Wang Y, Chang A, Haas M, Quigg R. Loss of CD11b exacerbates murine complement-mediated tubulointerstitial nephritis. PLoS ONE. 2014;9:e92051 pubmed publisher
    Acute complement activation occurs in the tubulointerstitium (TI) of kidneys transplanted from Crry(-/-)C3(-/-) mice into complement-sufficient wildtype mice, followed by marked inflammatory cell infiltration, tubular damage and ..
  24. Aegerter Shaw M, Cole J, Klickstein L, Wong W, Fearon D, Lalley P, et al. Expansion of the complement receptor gene family. Identification in the mouse of two new genes related to the CR1 and CR2 gene family. J Immunol. 1987;138:3488-94 pubmed
  25. Kohchi C, Noguchi K, Tanabe Y, Mizuno D, Soma G. Constitutive expression of TNF-alpha and -beta genes in mouse embryo: roles of cytokines as regulator and effector on development. Int J Biochem. 1994;26:111-9 pubmed
    ..Among them, the expression of C3 and CR1 were prominent. These results strongly support our idea that inflammation-like system play an important role to regulate embryogenesis. ..
  26. Mehrabian M, Qiao J, Hyman R, Ruddle D, Laughton C, Lusis A. Influence of the apoA-II gene locus on HDL levels and fatty streak development in mice. Arterioscler Thromb. 1993;13:1-10 pubmed
    ..The results support the concept that increased synthesis of apoAII results in increased HDL cholesterol levels. Unexpectedly, increased expression of apoAII appeared to promote rather than retard aortic fatty streak development. ..
  27. Chen Y, Perry D, Boackle S, Sobel E, Molina H, Croker B, et al. Several genes contribute to the production of autoreactive B and T cells in the murine lupus susceptibility locus Sle1c. J Immunol. 2005;175:1080-9 pubmed
  28. Allen J, Jaffer Z, Park S, Burgin S, Hofmann C, Sells M, et al. p21-activated kinase regulates mast cell degranulation via effects on calcium mobilization and cytoskeletal dynamics. Blood. 2009;113:2695-705 pubmed publisher
    ..These data implicate Pak1 as an essential molecular target for modulating acute mast cell responses that contribute to allergic diseases. ..
  29. Yin X, Zhou J. [Hepatitis B virus X protein upregulates the expression of CD59 and Crry in mouse podocytes]. Zhonghua Er Ke Za Zhi. 2010;48:934-8 pubmed
    ..The aim of this study was to investigate the influence of hepatitis B x protein (HBx) on the expression of CD59 and Crry in mouse podocytes...
  30. Tchepeleva S, Thurman J, Ruff K, Perkins S, Morel L, Boackle S. An allelic variant of Crry in the murine Sle1c lupus susceptibility interval is not impaired in its ability to regulate complement activation. J Immunol. 2010;185:2331-9 pubmed publisher
    ..CR1-related protein/gene Y (Crry) lies 10 kb from Cr2 and encodes a ubiquitously expressed complement regulatory protein that could also play a role ..
  31. Grizenkova J, Akhtar S, Collinge J, Lloyd S. The retinoic acid receptor beta (Rarb) region of Mmu14 is associated with prion disease incubation time in mouse. PLoS ONE. 2010;5:e15019 pubmed publisher
    ..04) and Rarb (P?=?0.0005), however, this was only highly significant for Rarb. This data provides significant further support for a role for the Rarb region of Mmu14 and Stmn2 in prion disease. ..
  32. Spitzer D, Unsinger J, Mao D, Wu X, Molina H, Atkinson J. In vivo correction of complement regulatory protein deficiency with an inhibitor targeting the red blood cell membrane. J Immunol. 2005;175:7763-70 pubmed
    ..In a model system in which RBCs deficient in complement receptor 1-related gene/protein y (Crry) are rapidly cleared after injection into wild-type animals by a complement-dependent mechanism, we selectively ..
  33. Kurtz C, Paul M, Aegerter M, Weis J, Weis J. Murine complement receptor gene family. II. Identification and characterization of the murine homolog (Cr2) to human CR2 and its molecular linkage to Crry. J Immunol. 1989;143:2058-67 pubmed
    ..Use of these murine Cr2-specific cDNA clones to isolate their respective genomic sequences has allowed for the linkage of the 3' end of the Cr2 gene to the 5' end of the Crry gene, the evolutionary homolog to human CR1.
  34. Rehrig S, Fleming S, Anderson J, Guthridge J, Rakstang J, McQueen C, et al. Complement inhibitor, complement receptor 1-related gene/protein y-Ig attenuates intestinal damage after the onset of mesenteric ischemia/reperfusion injury in mice. J Immunol. 2001;167:5921-7 pubmed
    Complement receptor 1-related gene/protein y (Crry) is a murine membrane protein that regulates the activity of both classical and alternative complement pathways...
  35. Bao L, Haas M, Boackle S, Kraus D, Cunningham P, Park P, et al. Transgenic expression of a soluble complement inhibitor protects against renal disease and promotes survival in MRL/lpr mice. J Immunol. 2002;168:3601-7 pubmed
    ..Production of sCrry in sera led to significant complement inhibition in Crry-transgenic mice relative to littermate transgene negative controls...
  36. Atkinson C, Zhu H, Qiao F, Varela J, Yu J, Song H, et al. Complement-dependent P-selectin expression and injury following ischemic stroke. J Immunol. 2006;177:7266-74 pubmed
    ..Complement receptor 2 (CR2)-Crry inhibits complement activation at the C3 stage and targets to sites of complement activation...
  37. Laskowski J, Renner B, Le Quintrec M, Panzer S, Hannan J, Ljubanovic D, et al. Distinct roles for the complement regulators factor H and Crry in protection of the kidney from injury. Kidney Int. 2016;90:109-22 pubmed publisher
    ..To examine the roles of the complement regulatory proteins factor H and Crry in protecting distinct renal surfaces from alternative pathway mediated injury, we generated mice with targeted ..
  38. Ojeda G, Pini E, Eguiluz C, Montes Casado M, Broere F, van Eden W, et al. Complement regulatory protein Crry/p65 costimulation expands natural treg cells with enhanced suppressive properties in proteoglycan-induced arthritis. Arthritis Rheum. 2011;63:1562-72 pubmed publisher
    ..These results highlight the potential of the complement regulatory protein Crry to costimulate and expand natural Treg cells capable of suppressing disease in an animal model of ..
  39. Kimura Y, Zhou L, Miwa T, Song W. Genetic and therapeutic targeting of properdin in mice prevents complement-mediated tissue injury. J Clin Invest. 2010;120:3545-54 pubmed
    ..mice from AP complement–mediated embryonic lethality caused by deficiency of the membrane complement regulator Crry and markedly reduced disease severity in the K/BxN model of arthritis...
  40. Turnberg D, Botto M. The regulation of the complement system: insights from genetically-engineered mice. Mol Immunol. 2003;40:145-53 pubmed
    ..The advantages and disadvantages of this type of approach are discussed and the insights gained from the investigation of these animals are reviewed. ..
  41. Bao L, Wang Y, Chen P, Sarav M, Haas M, Minto A, et al. Mesangial cell complement receptor 1-related protein y limits complement-dependent neutrophil accumulation in immune complex glomerulonephritis. Immunology. 2009;128:e895-904 pubmed publisher
    The absence of complement receptor 1 (CR1) related gene/protein y (Crry) leads to embryonic lethality as a result of unrestricted complement activation and concomitant neutrophil infiltration...
  42. Li Q, Nacion K, Bu H, Lin F. Mouse CD4+ CD25+ T regulatory cells are protected from autologous complement mediated injury by Crry and CD59. Biochem Biophys Res Commun. 2009;382:223-6 pubmed publisher
    ..We report here that mouse Treg cells express virtually no DAF or CR1. Instead, all of them express Crry and approximately half of them express CD59...
  43. Spitzer D, Wu X, Ma X, Xu L, Ponder K, Atkinson J. Cutting edge: treatment of complement regulatory protein deficiency by retroviral in vivo gene therapy. J Immunol. 2006;177:4953-6 pubmed
    ..The fusion gene containing the mouse C receptor 1-related gene/protein y (Crry) and a single chain Ab fragment with specificity for mouse glycophorin A was placed under transcriptional control ..
  44. Kimura Y, Miwa T, Zhou L, Song W. Activator-specific requirement of properdin in the initiation and amplification of the alternative pathway complement. Blood. 2008;111:732-40 pubmed
    ..AP complement activation was abolished in properdin-/- mouse serum, and properdin-/- mice were unable to clear Crry-deficient erythrocytes, which are known to be susceptible to AP complement-mediated extravascular hemolysis...
  45. Rancan M, Morganti Kossmann M, Barnum S, Saft S, Schmidt O, Ertel W, et al. Central nervous system-targeted complement inhibition mediates neuroprotection after closed head injury in transgenic mice. J Cereb Blood Flow Metab. 2003;23:1070-4 pubmed
    ..This work further implicates the complement system as a participant in secondary progression of brain damage after head trauma and provides a strong rationale for future studies of posttraumatic pharmacologic complement inhibition...
  46. Atkinson C, He S, Morris K, Qiao F, Casey S, Goddard M, et al. Targeted complement inhibitors protect against posttransplant cardiac ischemia and reperfusion injury and reveal an important role for the alternative pathway of complement activation. J Immunol. 2010;185:7007-13 pubmed publisher
    ..heterotopic isograft heart transplants were performed in C57BL/6 mice treated with a single injection of either CR2-Crry (inhibits all complement pathways) or CR2-fH (inhibits alternative complement pathway) immediately ..
  47. Takeshita A, Nagaishi S, Kondo T, Okada T, Kusakabe K. Regulation of complement activity via the alternative pathway in placentas of mouse spontaneous abortions. J Vet Med Sci. 2010;72:1375-7 pubmed
    ..Western blotting revealed that Crry was clearly present in the placentas from abortions...
  48. Taketo M, Howard T, Seldin M. Mapping of recombinant retrovirus integration sites that cause expression of the viral genome in murine embryonal carcinoma cells. Mamm Genome. 1992;2:240-5 pubmed
    ..None of these loci appear to be linked to any known Mo-MuLV proviral integration sites previously mapped. These enhancer and promoter loci may represent a new set of genes active in undifferentiated embryonic cells. ..
  49. Davoust N, Nataf S, Reiman R, Holers M, Campbell I, Barnum S. Central nervous system-targeted expression of the complement inhibitor sCrry prevents experimental allergic encephalomyelitis. J Immunol. 1999;163:6551-6 pubmed
    ..Furthermore, this work underscores the importance of humoral immunity in amplifying a T cell-initiated pathogenic process. ..
  50. Killick R, Hughes T, Morgan B, Lovestone S. Deletion of Crry, the murine ortholog of the sporadic Alzheimer's disease risk gene CR1, impacts tau phosphorylation and brain CFH. Neurosci Lett. 2013;533:96-9 pubmed publisher
    ..In rodents the closest ortholog of CR1 is the Crry gene (Cr1-related protein Y)...
  51. Martin B, Weis J. Functional identification of transcription control sequences of the mouse Crry gene. J Immunol. 1993;151:857-69 pubmed
    The mouse C receptor-related gene Crry is expressed by a wide variety of cells. Those sequences required for the transcriptional control of this gene were identified by deletion analysis of regions 5' of the initiating ATG...
  52. Paul M, Aegerter M, O Brien S, Kurtz C, Weis J. The murine complement receptor gene family. Analysis of mCRY gene products and their homology to human CR1. J Immunol. 1989;142:582-9 pubmed
    ..These genes, termed murine CRY (mCRY) and murine CRX (mCRX) reside on murine chromosomes 1 and 8, respectively...