Gene Symbol: Cnot7
Description: CCR4-NOT transcription complex, subunit 7
Alias: AU022737, CAF-1, Caf1, Pop2, CCR4-NOT transcription complex subunit 7, CCR4-associated factor 1, POP2 homolog, catabolite repressor protein (CCR4)-associative factor 1
Species: mouse
Products:     Cnot7

Top Publications

  1. Nakamura T, Yao R, Ogawa T, Suzuki T, Ito C, Tsunekawa N, et al. Oligo-astheno-teratozoospermia in mice lacking Cnot7, a regulator of retinoid X receptor beta. Nat Genet. 2004;36:528-33 pubmed
    ..Here we show that Cnot7(-/-) males are sterile owing to oligo-astheno-teratozoospermia, suggesting that Cnot7, a CCR4-associated ..
  2. Viswanathan P, Ohn T, Chiang Y, Chen J, Denis C. Mouse CAF1 can function as a processive deadenylase/3'-5'-exonuclease in vitro but in yeast the deadenylase function of CAF1 is not required for mRNA poly(A) removal. J Biol Chem. 2004;279:23988-95 pubmed
    The mouse CAF1 (mCAF1) is an ortholog of the yeast (y) CAF1 protein, which is a component of the CCR4-NOT complex, the major cytoplasmic deadenylase of Saccharomyces cerevisiae...
  3. Suzuki A, Igarashi K, Aisaki K, Kanno J, Saga Y. NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs. Proc Natl Acad Sci U S A. 2010;107:3594-9 pubmed publisher
    ..We propose from our current findings that NANOS2-interacting RNAs may be recruited to P-bodies and degraded by the enzymes contained therein through NANOS2-mediated deadenylation. ..
  4. Morita M, Suzuki T, Nakamura T, Yokoyama K, Miyasaka T, Yamamoto T. Depletion of mammalian CCR4b deadenylase triggers elevation of the p27Kip1 mRNA level and impairs cell growth. Mol Cell Biol. 2007;27:4980-90 pubmed
    ..Thus, CCR4b may be involved in various cellular events that include cell proliferation. ..
  5. Zheng D, Ezzeddine N, Chen C, Zhu W, He X, Shyu A. Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells. J Cell Biol. 2008;182:89-101 pubmed publisher
    ..We identify Pan2, Pan3, and Caf1 deadenylases as new P-body components and show that Pan3 helps recruit Pan2, Ccr4, and Caf1 to P-bodies...
  6. Albert T, Lemaire M, van Berkum N, Gentz R, Collart M, Timmers H. Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res. 2000;28:809-17 pubmed
    ..It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional proteins like yeast CAF1. Here we report the isolation of cDNAs encoding human NOT2, NOT3, NOT4 and a CAF1-like factor, CALIF...
  7. Rouault J, Prevot D, Berthet C, Birot A, Billaud M, Magaud J, et al. Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex. J Biol Chem. 1998;273:22563-9 pubmed
    ..Finally, we found that box B, one of two conserved domains which define the Btg family, plays a functional role, namely that it is essential to the Btg-mCaf1 interaction. ..
  8. Berthet C, Morera A, Asensio M, Chauvin M, Morel A, Dijoud F, et al. CCR4-associated factor CAF1 is an essential factor for spermatogenesis. Mol Cell Biol. 2004;24:5808-20 pubmed
    The CCR4-associated protein CAF1 has been demonstrated to play several roles in the control of transcription and of mRNA decay. To gain further insight into its physiological function, we generated CAF1-deficient mice...
  9. Ma J, Fukuda Y, Schultz R. Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation. Biol Reprod. 2015;93:48 pubmed publisher
    ..We report here that Cnot7, Cnot6l, and Pan2, key components of deadenylation machinery, are also dormant maternal mRNAs that are recruited ..

More Information


  1. Yu C, Ji S, Sha Q, Dang Y, Zhou J, Zhang Y, et al. BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes. Nat Struct Mol Biol. 2016;23:387-94 pubmed publisher
    ..Here, we identified B-cell translocation gene-4 (BTG4) as an MZT licensing factor in mice. BTG4 bridged CNOT7, a catalytic subunit of the CCR4-NOT deadenylase, to eIF4E, a key translation initiation factor, and facilitated ..
  2. Fabian M, Mathonnet G, Sundermeier T, Mathys H, Zipprich J, Svitkin Y, et al. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell. 2009;35:868-80 pubmed publisher
    ..let-7 miRNA-loaded RNA-induced silencing complex (miRISC) interacts with the poly(A)-binding protein (PABP) and the CAF1 and CCR4 deadenylases...
  3. Mauxion F, Preve B, Seraphin B. C2ORF29/CNOT11 and CNOT10 form a new module of the CCR4-NOT complex. RNA Biol. 2013;10:267-76 pubmed publisher
    ..Thus, the human CCR4-NOT complex possesses in addition to the CCR4-CAF1 deadenylase module and to the NOT module, a module composed of CNOT10 and CNOT11 that interacts with the N-terminal ..
  4. Faraji F, Hu Y, Yang H, Lee M, Winkler G, Hafner M, et al. Post-transcriptional Control of Tumor Cell Autonomous Metastatic Potential by CCR4-NOT Deadenylase CNOT7. PLoS Genet. 2016;12:e1005820 pubmed publisher
    ..and genetically engineered mouse models, we show that one of the enzymatic subunits of the CCR4-NOT complex, Cnot7, is also a metastasis modifying gene...
  5. Mauxion F, Faux C, Seraphin B. The BTG2 protein is a general activator of mRNA deadenylation. EMBO J. 2008;27:1039-48 pubmed publisher
    ..BTG2 has also been shown to interact with the Pop2/Caf1 deadenylase...
  6. Ogawa T, Ito C, Nakamura T, Tamura Y, Yamamoto T, Noda T, et al. Abnormal sperm morphology caused by defects in Sertoli cells of Cnot7 knockout mice. Arch Histol Cytol. 2004;67:307-14 pubmed
    b>Cnot7 is a co-factor of transcription regulation, expressed in a variety of tissues including the lung, liver, thyroid gland, and testis. Our previous study (Nakamura et al...
  7. Pasternak M, Pfender S, Santhanam B, Schuh M. The BTG4 and CAF1 complex prevents the spontaneous activation of eggs by deadenylating maternal mRNAs. Open Biol. 2016;6: pubmed publisher
    ..Here, we report that a complex of BTG4 and CAF1 safeguards metaphase II arrest in mammalian eggs by deadenylating maternal mRNAs...
  8. Draper M, Salvadore C, Denis C. Identification of a mouse protein whose homolog in Saccharomyces cerevisiae is a component of the CCR4 transcriptional regulatory complex. Mol Cell Biol. 1995;15:3487-95 pubmed
    ..Both yCAF1 (POP2) and the C. elegans homolog of CAF1 were shown to genetically interact with CCR4 in vivo, and yCAF1 (POP2) physically associated with CCR4...
  9. Levy R, Mott R, Iraqi F, Gabet Y. Collaborative cross mice in a genetic association study reveal new candidate genes for bone microarchitecture. BMC Genomics. 2015;16:1013 pubmed publisher
    ..10(-6)) and which have a validated causal role in skeletal biology, are Avp, Oxt, B2m (associated with BV/TV), Cnot7 (with Tb.N), Pcsk6, Rgma (with Tb.Th), Rb1, and Cpb2 (with Conn.D)...
  10. Huang K, Chadee A, Chen C, Zhang Y, Shyu A. Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate. RNA. 2013;19:295-305 pubmed publisher
    ..Taken together, these results provide a new framework for understanding the roles of intrinsically disordered proteins in the dynamic and signal-dependent control of cytoplasmic mRNA functions. ..
  11. McFleder R, Mansur F, Richter J. Dynamic Control of Dendritic mRNA Expression by CNOT7 Regulates Synaptic Efficacy and Higher Cognitive Function. Cell Rep. 2017;20:683-696 pubmed publisher
    ..Here, we find that the deadenylase CNOT7 dynamically regulates dendritic mRNA translation and transport, as well as synaptic plasticity and higher ..
  12. Suzuki A, Saba R, Miyoshi K, Morita Y, Saga Y. Interaction between NANOS2 and the CCR4-NOT deadenylation complex is essential for male germ cell development in mouse. PLoS ONE. 2012;7:e33558 pubmed publisher
  13. Morita M, Oike Y, Nagashima T, Kadomatsu T, Tabata M, Suzuki T, et al. Obesity resistance and increased hepatic expression of catabolism-related mRNAs in Cnot3+/- mice. EMBO J. 2011;30:4678-91 pubmed publisher
    ..Finally, as CNOT3 levels in the liver and white adipose tissues decrease upon fasting, we propose that CNOT3 responds to feeding conditions to regulate deadenylation-specific mRNAs and energy metabolism. ..
  14. Chen C, Ito K, Takahashi A, Wang G, Suzuki T, Nakazawa T, et al. Distinct expression patterns of the subunits of the CCR4-NOT deadenylase complex during neural development. Biochem Biophys Res Commun. 2011;411:360-4 pubmed publisher
    ..These findings suggest that subunit composition of the CCR4-NOT complex differs among tissues and is altered during neural development, thereby imparting an additional layer of specificity in the control of gene expression. ..
  15. Marchese F, Aubareda A, Tudor C, Saklatvala J, Clark A, Dean J. MAPKAP kinase 2 blocks tristetraprolin-directed mRNA decay by inhibiting CAF1 deadenylase recruitment. J Biol Chem. 2010;285:27590-600 pubmed publisher
    ..find that MK2 phosphorylation reduces the ability of TTP to promote deadenylation by inhibiting the recruitment of CAF1 deadenylase in a mechanism that does not involve sequestration of TTP by 14-3-3...
  16. Chen C, Zheng D, Xia Z, Shyu A. Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps. Nat Struct Mol Biol. 2009;16:1160-6 pubmed publisher
    ..GW182), trigger very rapid mRNA decay by inducing accelerated biphasic deadenylation mediated by Pan2-Pan3 and Ccr4-Caf1 deadenylase complexes followed by Dcp1-Dcp2 complex-directed decapping in mammalian cells...
  17. Washio Oikawa K, Nakamura T, Usui M, Yoneda M, Ezura Y, Ishikawa I, et al. Cnot7-null mice exhibit high bone mass phenotype and modulation of BMP actions. J Bone Miner Res. 2007;22:1217-23 pubmed
    b>Cnot7 is a recently identified regulator of spermatogenesis in adult mice...
  18. Washio Oikawa K, Nakamura T, Usui M, Yoneda M, Ezura Y, Ishikawa I, et al. Expression analysis of LacZ gene placed in the locus of Cnot7 exhibits its activity in osteoblasts in vivo and in mineralized nodules in vitro. J Cell Biochem. 2006;99:538-44 pubmed
    CCR4-NOT complex 7 (Cnot7) was identified as a regulator of gene expression in yeast and evolutionally conserved in mammals. Cnot7 deficient male mice exhibit abnormality in spermatogenesis...