Gene Symbol: Chka
Description: choline kinase alpha
Alias: CK/EK-alpha, Chetk-alpha, Chk, ChoK, EtnK-alpha, choline kinase alpha, choline/ethanolamine kinase alpha, ethanolamine kinase
Species: mouse
Products:     Chka

Top Publications

  1. Liao H, Aoyama C, Ishidate K, Teraoka H. Deletion and alanine mutation analyses for the formation of active homo- or hetero-dimer complexes of mouse choline kinase-alpha and -beta. Biochim Biophys Acta. 2006;1761:111-20 pubmed
    ..A mutation of the corresponding D276 of CK-beta to A276 did not show any effect on either its homo- or hetero-dimerization but it caused a strong inhibition of CK activity in either case. ..
  2. Wu G, Aoyama C, Young S, Vance D. Early embryonic lethality caused by disruption of the gene for choline kinase alpha, the first enzyme in phosphatidylcholine biosynthesis. J Biol Chem. 2008;283:1456-62 pubmed
    b>Choline kinase alpha (CK-alpha) is one of two mammalian enzymes that catalyze the phosphorylation of choline to phosphocholine in the biosynthesis of the major membrane phospholipid, phosphatidylcholine...
  3. Mintz L, Galperin E, Pasmanik Chor M, Tulzinsky S, Bromberg Y, Kozak C, et al. EHD1--an EH-domain-containing protein with a specific expression pattern. Genomics. 1999;59:66-76 pubmed
    ..The EHD1 gene is highly conserved between nematode, Drosophila, mouse, and human. Its predicted protein structure and cellular localization point to the possibility that EHD1 participates in ligand-induced endocytosis. ..
  4. Hurley T, McClive P, Sarraj M, Sinclair A. Eki2 is upregulated specifically in the testis during mouse sex determination. Gene Expr Patterns. 2004;4:135-40 pubmed
    ..a gene with gonad restricted expression throughout mouse development, which is orthologous to human EKI2 (ethanolamine kinase 2)...
  5. Gallego Ortega D, Ramirez de Molina A, Gutierrez R, Ramos M, Sarmentero J, Cejas P, et al. Generation and characterization of monoclonal antibodies against choline kinase alpha and their potential use as diagnostic tools in cancer. Int J Oncol. 2006;29:335-40 pubmed
    b>Choline kinase alpha (ChoKalpha) is a metabolic enzyme involved in the synthesis of phosphatidylcholine, recently implicated in cancer onset since it is overexpressed in a variety of human cancers such as mammary, lung, colorectal and ..
  6. Marcucci H, Paoletti L, Jackowski S, Banchio C. Phosphatidylcholine biosynthesis during neuronal differentiation and its role in cell fate determination. J Biol Chem. 2010;285:25382-93 pubmed publisher
    ..differentiation of Neuro-2a cells, PtdCho synthesis was promoted by an ordered and sequential activation of choline kinase alpha (CK(alpha)) and choline cytidylyltransferase alpha (CCT(alpha))...
  7. Tian Y, Jackson P, Gunter C, Wang J, Rock C, Jackowski S. Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2. J Biol Chem. 2006;281:28438-49 pubmed
    b>Ethanolamine kinase catalyzes the first step in the CDP-ethanolamine pathway for the formation of the major membrane phospholipid phosphatidylethanolamine (PtdEtn)...
  8. Gréchez Cassiau A, Feillet C, Guérin S, Delaunay F. The hepatic circadian clock regulates the choline kinase α gene through the BMAL1-REV-ERBα axis. Chronobiol Int. 2015;32:774-84 pubmed publisher
    ..pathways which control the production of hepatic phosphatidylcholine revealed a robust circadian pattern for Chkα while other mRNA showed low amplitude (Chkβ and Pemt) or no rhythm (Cctα and Chpt1)...
  9. Aoyama C, Yamazaki N, Terada H, Ishidate K. Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes alpha and beta. J Lipid Res. 2000;41:452-64 pubmed
    Choline/ethanolamine kinase (CK/EK) is the first enzyme in phosphatidylcholine/phosphatidylethanolamine biosynthesis in all animal cells...

More Information


  1. Sher R, Aoyama C, Huebsch K, Ji S, Kerner J, Yang Y, et al. A rostrocaudal muscular dystrophy caused by a defect in choline kinase beta, the first enzyme in phosphatidylcholine biosynthesis. J Biol Chem. 2006;281:4938-48 pubmed
    ..CHKB is one of two mammalian choline kinase (CHK) enzymes (alpha and beta) that catalyze the phosphorylation of choline to phosphocholine in the biosynthesis of the ..
  2. Wu G, Sher R, Cox G, Vance D. Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse. Biochim Biophys Acta. 2010;1801:446-54 pubmed publisher
    ..kinase beta is the major isoform in hindlimb muscle and contributes more to choline kinase activity, while choline kinase alpha is predominant in forelimb muscle and contributes more to choline kinase activity...
  3. Aoyama C, Ohtani A, Ishidate K. Expression and characterization of the active molecular forms of choline/ethanolamine kinase-alpha and -beta in mouse tissues, including carbon tetrachloride-induced liver. Biochem J. 2002;363:777-84 pubmed
    Choline/ethanolamine kinase (ChoK/EtnK) exists as at least three isoforms (alpha1, alpha2 and beta) in mammalian cells...
  4. Aoyama C, Ishidate K, Sugimoto H, Vance D. Induction of choline kinase alpha by carbon tetrachloride (CCl4) occurs via increased binding of c-jun to an AP-1 element. Biochim Biophys Acta. 2007;1771:1148-55 pubmed
    The mechanism by which treatment of mice with CCl4 induces an increase in choline kinase alpha has been investigated...
  5. Aoyama C, Nakashima K, Ishidate K. Molecular cloning of mouse choline kinase and choline/ethanolamine kinase: their sequence comparison to the respective rat homologs. Biochim Biophys Acta. 1998;1393:179-85 pubmed
    Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S. Yamashita, J. Biol. Chem...
  6. Deussing J, Tisljar K, Papazoglou A, Peters C. Mouse cathepsin F: cDNA cloning, genomic organization and chromosomal assignment of the gene. Gene. 2000;251:165-73 pubmed
    ..Northern blot analysis of RNA from multiple tissues revealed a ubiquitous expression of cathepsin F in mouse and man. ..