Cdk1

Summary

Gene Symbol: Cdk1
Description: cyclin-dependent kinase 1
Alias: Cdc2, Cdc2a, p34, cyclin-dependent kinase 1, cell cycle p34 CDC2 kinase protein, cell division control protein 2 homolog, cell division cycle 2 homolog A, cell division cycle control protein 2a, cell division protein kinase 1, p34 protein kinase
Species: mouse
Products:     Cdk1

Top Publications

  1. Berthet C, Klarmann K, Hilton M, Suh H, Keller J, Kiyokawa H, et al. Combined loss of Cdk2 and Cdk4 results in embryonic lethality and Rb hypophosphorylation. Dev Cell. 2006;10:563-73 pubmed
    ..a gradual decrease of Retinoblastoma protein (Rb) phosphorylation and reduced expression of E2F-target genes, like Cdc2 and cyclin A2, during embryogenesis and in embryonic fibroblasts (MEFs)...
  2. Kurita M, Kuwajima T, Nishimura I, Yoshikawa K. Necdin downregulates CDC2 expression to attenuate neuronal apoptosis. J Neurosci. 2006;26:12003-13 pubmed
    ..activates many proapoptotic genes including the cyclin-dependent protein kinase cell division cycle 2 (Cdc2)...
  3. Santamaria D, Barrière C, Cerqueira A, Hunt S, Tardy C, Newton K, et al. Cdk1 is sufficient to drive the mammalian cell cycle. Nature. 2007;448:811-5 pubmed
    Unicellular organisms such as yeasts require a single cyclin-dependent kinase, Cdk1, to drive cell division...
  4. Ullah Z, Kohn M, Yagi R, Vassilev L, DePamphilis M. Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activity. Genes Dev. 2008;22:3024-36 pubmed publisher
    ..Here we show that RO3306 inhibition of cyclin-dependent protein kinase 1 (CDK1), the enzyme required to enter mitosis, induced differentiation of TS cells into TG cells...
  5. Nabti I, Marangos P, Bormann J, Kudo N, Carroll J. Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis I progression and fidelity. J Cell Biol. 2014;204:891-900 pubmed publisher
    Female meiosis is driven by the activities of two major kinases, cyclin-dependent kinase 1 (Cdk1) and mitogen-activated protein kinase (MAPK)...
  6. Lane S, Yun Y, Jones K. Timing of anaphase-promoting complex activation in mouse oocytes is predicted by microtubule-kinetochore attachment but not by bivalent alignment or tension. Development. 2012;139:1947-55 pubmed publisher
    ..The time from when the APC/C became active until anaphase onset was affected by the rate of loss of CDK1 activity, rather than by these non-aligned bivalents, which occasionally persisted until anaphase, resulting in ..
  7. Davydenko O, Schultz R, Lampson M. Increased CDK1 activity determines the timing of kinetochore-microtubule attachments in meiosis I. J Cell Biol. 2013;202:221-9 pubmed publisher
    ..Here we show that during meiosis I, attachments are regulated by CDK1 activity, which gradually increases through prometaphase and metaphase I...
  8. Kalaszczynska I, Geng Y, Iino T, Mizuno S, Choi Y, Kondratiuk I, et al. Cyclin A is redundant in fibroblasts but essential in hematopoietic and embryonic stem cells. Cell. 2009;138:352-65 pubmed publisher
    ..Expression of cyclin A is particularly high in these compartments, which might render stem cells dependent on cyclin A, whereas in fibroblasts cyclins A and E play redundant roles in cell proliferation. ..
  9. Shoji S, Yoshida N, Amanai M, Ohgishi M, Fukui T, Fujimoto S, et al. Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20. EMBO J. 2006;25:834-45 pubmed
    Fertilizable mammalian oocytes are arrested at the second meiotic metaphase (mII) by the cyclinB-Cdc2 heterodimer, maturation promoting factor (MPF)...

More Information

Publications99

  1. Kozar K, Ciemerych M, Rebel V, Shigematsu H, Zagozdzon A, Sicinska E, et al. Mouse development and cell proliferation in the absence of D-cyclins. Cell. 2004;118:477-91 pubmed
    ..Lastly, we found that cells lacking D-cyclins display reduced susceptibility to the oncogenic transformation. Our results reveal the presence of alternative mechanisms that allow cell cycle progression in a cyclin D-independent fashion. ..
  2. Satyanarayana A, Hilton M, Kaldis P. p21 Inhibits Cdk1 in the absence of Cdk2 to maintain the G1/S phase DNA damage checkpoint. Mol Biol Cell. 2008;19:65-77 pubmed
    b>Cdk1 was proposed to compensate for the loss of Cdk2. Here we present evidence that this is possible due to premature translocation of Cdk1 from the cytoplasm to the nucleus in the absence of Cdk2...
  3. Berthet C, Aleem E, Coppola V, Tessarollo L, Kaldis P. Cdk2 knockout mice are viable. Curr Biol. 2003;13:1775-85 pubmed
    ..Loss of Cdk2 affects the timing of S phase, suggesting that Cdk2 is involved in regulating progression through the mitotic cell cycle. ..
  4. Diril M, Ratnacaram C, Padmakumar V, Du T, Wasser M, Coppola V, et al. Cyclin-dependent kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration. Proc Natl Acad Sci U S A. 2012;109:3826-31 pubmed publisher
    Cyclin-dependent kinase 1 (Cdk1) is an archetypical kinase and a central regulator that drives cells through G2 phase and mitosis...
  5. Zhao J, Nornes H, Neuman T. Expression of Rb, E2F1, cdc2, and D, and B cyclins in developing spinal cord. Neurosci Lett. 1995;190:49-52 pubmed
    The spatial and temporal distribution of transcripts for the tumor suppressor gene Rb, transcription factor E2F1, cdc2 kinase, cyclins D1, D2, B1 and B2 during neurogenesis of the spinal cord was determined by in situ hybridization...
  6. Nandan M, Chanchevalap S, Dalton W, Yang V. Krüppel-like factor 5 promotes mitosis by activating the cyclin B1/Cdc2 complex during oncogenic Ras-mediated transformation. FEBS Lett. 2005;579:4757-62 pubmed
    ..parental NIH3T3 cells, H-Ras-transformed fibroblasts exhibit an increase in mitotic index, levels of cyclin B1 and Cdc2, and cyclin B1/Cdc2 kinase activity...
  7. Nakayama S, Torikoshi Y, Takahashi T, Yoshida T, Sudo T, Matsushima T, et al. Prediction of paclitaxel sensitivity by CDK1 and CDK2 activity in human breast cancer cells. Breast Cancer Res. 2009;11:R12 pubmed publisher
    ..We then examined the specific activities of CDK1 and CDK2 in these cell lines and in xenograft models of human breast cancer before and after paclitaxel treatment...
  8. Xu X, Zhang Z, Su W, Zhang Y, Yu Y, Li Y, et al. Characterization of p70 S6 kinase 1 in early development of mouse embryos. Dev Dyn. 2009;238:3025-34 pubmed publisher
    ..It is proposed that mTORC1 may be involved in the control of MPF by regulating S6K1 during the early development of mouse embryos. ..
  9. Niida H, Tsuge S, Katsuno Y, Konishi A, Takeda N, Nakanishi M. Depletion of Chk1 leads to premature activation of Cdc2-cyclin B and mitotic catastrophe. J Biol Chem. 2005;280:39246-52 pubmed
    ..Chk1 deficiency resulted in a premature onset of mitosis because of abnormal activation of cyclin B-Cdc2 and led to the activation of caspases 3 and 9 triggered by cytoplasmic release of cytochrome c...
  10. Miettinen T, Pessa H, Caldez M, Fuhrer T, Diril M, Sauer U, et al. Identification of transcriptional and metabolic programs related to mammalian cell size. Curr Biol. 2014;24:598-608 pubmed publisher
    ..Mitochondrial metabolism and lipid synthesis are used to couple cell size and cell proliferation. This regulatory mechanism may provide a possible mechanism for sensing metazoan cell size...
  11. Andrecht S, Kolbus A, Hartenstein B, Angel P, Schorpp Kistner M. Cell cycle promoting activity of JunB through cyclin A activation. J Biol Chem. 2002;277:35961-8 pubmed
    ..This delay in G(2)/M-transition is caused by impaired cyclin A-CDK2 and cyclin B-CDC2 kinase activities and counteracts the accelerated S-phase entry...
  12. Zhang Z, Su W, Feng C, Yu D, Cui C, Xu X, et al. Polo-like kinase 1 may regulate G2/M transition of mouse fertilized eggs by means of inhibiting the phosphorylation of Tyr 15 of Cdc2. Mol Reprod Dev. 2007;74:1247-54 pubmed
    ..At the meantime, the phosphorylation of Tyr 15 of Cdc2 was inhibited at M phase...
  13. Li L, Wang J, Hou J, Wu Z, Zhuang Y, Lu M, et al. Cdk1 interplays with Oct4 to repress differentiation of embryonic stem cells into trophectoderm. FEBS Lett. 2012;586:4100-7 pubmed publisher
    b>Cdk1 plays an important role in undifferentiated ES cells, but the underlying mechanism remains unclear. This study explores how Cdk1 collaborates with Oct4 to inhibit differentiation in mouse ES cells...
  14. Wang G, Shi X, Haefliger S, Jin J, Major A, Iakova P, et al. Elimination of C/EBPalpha through the ubiquitin-proteasome system promotes the development of liver cancer in mice. J Clin Invest. 2010;120:2549-62 pubmed publisher
    ..This study identifies a molecular mechanism that supports the development of liver cancer in older mice and potential therapeutic targets for the prevention of liver cancer. ..
  15. Hu J, Lu J, Lian G, Zhang J, Hecht J, Sheen V. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS ONE. 2014;9:e89352 pubmed publisher
    ..FlnB loss reduced Cdk1 phosphorylation (an inhibitor of G2/M phase progression) and Cdk1 inhibition in chondrocytes mimicked the null FlnB,..
  16. Schmetsdorf S, Gärtner U, Arendt T. Expression of cell cycle-related proteins in developing and adult mouse hippocampus. Int J Dev Neurosci. 2005;23:101-12 pubmed
    ..Thus, they may have supplementary functions in differentiated neurons that might be associated with neuronal plasticity. ..
  17. Hirai H, Roussel M, Kato J, Ashmun R, Sherr C. Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Mol Cell Biol. 1995;15:2672-81 pubmed
    ..the kinase activities of CDK4 and CDK6, but do not affect those of cyclin E-CDK2, cyclin A-CDK2, or cyclin B-CDC2, were isolated...
  18. Ino H, Ishizuka T, Chiba T, Tatibana M. Expression of CDK5 (PSSALRE kinase), a neural cdc2-related protein kinase, in the mature and developing mouse central and peripheral nervous systems. Brain Res. 1994;661:196-206 pubmed
    CDK5 is a cdc2-related protein kinase that is known to be highly expressed in mature brain. In this study, we obtained a mouse CDK5 cDNA by screening an adult mouse cDNA library...
  19. Otero J, Kalaszczynska I, Michowski W, Wong M, Gygli P, Gokozan H, et al. Cerebellar cortical lamination and foliation require cyclin A2. Dev Biol. 2014;385:328-39 pubmed publisher
    ..Although cyclin E1 has been reported to compensate for cyclin A2 function in fibroblasts and is upregulated in cyclin A2 null cerebella, cyclin E1 expression was unable to compensate for loss-of cyclin A2 function. ..
  20. Forester C, Maddox J, Louis J, Goris J, Virshup D. Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1. Proc Natl Acad Sci U S A. 2007;104:19867-72 pubmed
    Inactivation of maturation-promoting factor [(MPF) Cdk1/Cyclin B] is a key event in the exit from mitosis...
  21. Pan J, Zou J, Wu D, Roberson R, Hennings L, Ma X, et al. TLS-ERG leukemia fusion protein deregulates cyclin-dependent kinase 1 and blocks terminal differentiation of myeloid progenitor cells. Mol Cancer Res. 2008;6:862-72 pubmed publisher
    ..We found that the level of cyclin-dependent kinase 1 (Cdk1) protein was significantly decreased in controls but unchanged in TLS-ERG-expressing cells after granulocyte colony-..
  22. Poon R, Chau M, Yamashita K, Hunter T. The role of Cdc2 feedback loop control in the DNA damage checkpoint in mammalian cells. Cancer Res. 1997;57:5168-78 pubmed
    ..damage, the G1-S CDKs are inhibited by a mechanism involving p53-dependent induction of p21Cip1/Waf1; but how the Cdc2 is inhibited is less apparent...
  23. Vecchione A, Baldassarre G, Ishii H, Nicoloso M, Belletti B, Petrocca F, et al. Fez1/Lzts1 absence impairs Cdk1/Cdc25C interaction during mitosis and predisposes mice to cancer development. Cancer Cell. 2007;11:275-89 pubmed
    ..Lzts1(-/-) mouse embryo fibroblasts (MEFs), Cdc25C degradation was increased during M phase, resulting in decreased Cdk1 activity...
  24. Pomerantz Y, Elbaz J, Ben Eliezer I, Reizel Y, David Y, Galiani D, et al. From ubiquitin-proteasomal degradation to CDK1 inactivation: requirements for the first polar body extrusion in mouse oocytes. FASEB J. 2012;26:4495-505 pubmed publisher
    ..first polar body (PBI), depends on proteasomal degradation of cyclin B1 and securin and the subsequent respective CDK1 inactivation and chromosome segregation...
  25. Medvedev S, Yang J, Hecht N, Schultz R. CDC2A (CDK1)-mediated phosphorylation of MSY2 triggers maternal mRNA degradation during mouse oocyte maturation. Dev Biol. 2008;321:205-15 pubmed publisher
    ..We report that CDC2A (CDK1)-mediated phosphorylation of MSY2 triggers this transition...
  26. Wang X, Rao R, Kosakowska Cholody T, Masood M, Southon E, Zhang H, et al. Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol. 2009;184:143-58 pubmed publisher
    ..Thus, global compromise of ER and mitochondrial integrity caused by ceramide accumulation in CERT mutant mice primarily affects organogenesis rather than causing cell death via apoptotic pathways. ..
  27. Inaba H, Yamakawa D, Tomono Y, Enomoto A, Mii S, Kasahara K, et al. Regulation of keratin 5/14 intermediate filaments by CDK1, Aurora-B, and Rho-kinase. Biochem Biophys Res Commun. 2018;498:544-550 pubmed publisher
    ..that vimentin, GFAP, and desmin (type III intermediate filament [IF] proteins) are mitotically phosphorylated by CDK1, Aurora-B, and Rho-kinase...
  28. Matsuo T, Yamaguchi S, Mitsui S, Emi A, Shimoda F, Okamura H. Control mechanism of the circadian clock for timing of cell division in vivo. Science. 2003;302:255-9 pubmed
    ..clock controls the expression of cell cycle-related genes that in turn modulate the expression of active Cyclin B1-Cdc2 kinase, a key regulator of mitosis...
  29. Pathan N, Geahlen R, Harrison M. The protein-tyrosine kinase Lck associates with and is phosphorylated by Cdc2. J Biol Chem. 1996;271:27517-23 pubmed
    ..Using antibodies to Lck and to the cyclin-dependent serine kinase, Cdc2, as well as the cyclin-dependent kinase affinity resin, Suc1-agarose, we detected a stable interaction between Lck ..
  30. Li B, Nair M, Mackay D, Bilanchone V, Hu M, Fallahi M, et al. Ovol1 regulates meiotic pachytene progression during spermatogenesis by repressing Id2 expression. Development. 2005;132:1463-73 pubmed
    ..Taken together, our studies demonstrate a role for Ovol1 in regulating pachytene progression of male germ cells, and identify Id2 as a Ovol1 target. ..
  31. Silva R, Dautel M, Di Genova B, Amberg D, Castilho B, Sattlegger E. The Gcn2 Regulator Yih1 Interacts with the Cyclin Dependent Kinase Cdc28 and Promotes Cell Cycle Progression through G2/M in Budding Yeast. PLoS ONE. 2015;10:e0131070 pubmed publisher
    ..We further show that IMPACT, the mammalian homologue of Yih1, interacts with CDK1, the mammalian counterpart of Cdc28, indicating that the involvement with the cell cycle is conserved...
  32. Huskey N, Guo T, Evason K, Momcilovic O, Pardo D, Creasman K, et al. CDK1 inhibition targets the p53-NOXA-MCL1 axis, selectively kills embryonic stem cells, and prevents teratoma formation. Stem Cell Reports. 2015;4:374-89 pubmed publisher
    ..We found that inhibiting cyclin-dependent kinase 1 (CDK1) leads to activation of the DNA damage response, nuclear p53 stabilization, activation of a subset of p53 target ..
  33. Nakajima T, Kitagawa K, Ohhata T, Sakai S, Uchida C, Shibata K, et al. Regulation of GATA-binding protein 2 levels via ubiquitin-dependent degradation by Fbw7: involvement of cyclin B-cyclin-dependent kinase 1-mediated phosphorylation of THR176 in GATA-binding protein 2. J Biol Chem. 2015;290:10368-81 pubmed publisher
    ..The CPD kinase, which mediates the phosphorylation of Thr(176), was cyclin B-cyclin-dependent kinase 1 (CDK1). Moreover, depletion of endogenous Fbw7 stabilized endogenous GATA2 in K562 cells...
  34. Godet M, Damestoy A, Mouradian S, Rudkin B, Durand P. Key role for cyclin-dependent kinases in the first and second meiotic divisions of rat spermatocytes. Biol Reprod. 2004;70:1147-52 pubmed
    ..examined so far, the G2/M phase transition is controlled by the M-phase promoting factor (MPF), a complex of cdc2 (CDK1) and cyclin B1. Histone H1 kinase activity and MPF components are present in pachytene spermatocytes (PS)...
  35. Kim M, Lee J, Lee S. Transient suppression of nuclear Cdc2 activity in response to ionizing radiation. Oncol Rep. 2008;19:1323-9 pubmed
    Suppression of Cdc2 activity is essential for DNA damage-induced G2 arrest. In the present study, we elucidated regulatory mechanism of Cdc2 activity during radiation-induced transient G2 arrest...
  36. Liu D, Matzuk M, Sung W, Guo Q, Wang P, Wolgemuth D. Cyclin A1 is required for meiosis in the male mouse. Nat Genet. 1998;20:377-80 pubmed
    ..in Ccna1-/- males was associated with increased germ cell apoptosis, desynapsis abnormalities and reduction of Cdc2 kinase activation at the end of meiotic prophase...
  37. Walker M, DiAugustine R, Zeringue E, Bunger M, Schmitt M, Archer T, et al. An IGF1/insulin receptor substrate-1 pathway stimulates a mitotic kinase (cdk1) in the uterine epithelium during the proliferative response to estradiol. J Endocrinol. 2010;207:225-35 pubmed publisher
    ..on earlier studies, it is also possible that an IGF1/IRS-1/PI3K/AKT pathway regulates posttranslational changes in cdk1. This model may provide insights as to how a growth factor pathway can mediate hormone action on cell proliferation. ..
  38. Castro D, Martynoga B, Parras C, Ramesh V, Pacary E, Johnston C, et al. A novel function of the proneural factor Ascl1 in progenitor proliferation identified by genome-wide characterization of its targets. Genes Dev. 2011;25:930-45 pubmed publisher
  39. Arai S, Miyake K, Voit R, Nemoto S, Wakeland E, Grummt I, et al. Death-effector domain-containing protein DEDD is an inhibitor of mitotic Cdk1/cyclin B1. Proc Natl Acad Sci U S A. 2007;104:2289-94 pubmed
    ..Here we identified that DEDD, which also possesses the DED domain, acts as a novel inhibitor of the mitotic Cdk1/cyclin B1 complex...
  40. El Dika M, Laskowska Kaszub K, Koryto M, Dudka D, Prigent C, Tassan J, et al. CDC6 controls dynamics of the first embryonic M-phase entry and progression via CDK1 inhibition. Dev Biol. 2014;396:67-80 pubmed publisher
    ..It also regulates M-phase exit by inhibiting the activity of the major M-phase protein kinase CDK1. Here we show that addition of recombinant CDC6 to Xenopus embryo cycling extract delays the M-phase entry and ..
  41. Jayapal S, Wang C, Bisteau X, Caldez M, Lim S, Tergaonkar V, et al. Hematopoiesis specific loss of Cdk2 and Cdk4 results in increased erythrocyte size and delayed platelet recovery following stress. Haematologica. 2015;100:431-8 pubmed publisher
    ..phosphorylation of the retinoblastoma protein and reduced expression of E2F target genes such as cyclin A2 and Cdk1. Erythroblasts lacking Cdk2 and Cdk4 displayed a lengthened G1 phase due to impaired phosphorylation of the ..
  42. Surmacz E, Nugent P, Pietrzkowski Z, Baserga R. The role of the IGF1 receptor in the regulation of cdc2 mRNA levels in fibroblasts. Exp Cell Res. 1992;199:275-8 pubmed
    The levels of cdc2 mRNA increase when quiescent cells are stimulated by growth factors. In BALB/c 3T3, both platelet-derived growth factor and insulin-like growth factor 1 (IGF-1) are required to increase cdc2 mRNA levels...
  43. Wells J, Boyd K, Fry C, Bartley S, Farnham P. Target gene specificity of E2F and pocket protein family members in living cells. Mol Cell Biol. 2000;20:5797-807 pubmed
  44. Zhu D, Dix D, Eddy E. HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes. Development. 1997;124:3007-14 pubmed
    Cyclin B-dependent CDC2 kinase activity has a key role in triggering the G2/M-phase transition during the mitotic and meiotic cell cycles. The Hsp70-2 gene is expressed only in spermatogenic cells at a significant level...
  45. Ahmed M, Alam M, Emelianov V, Poterlowicz K, Patel A, Sharov A, et al. MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway. J Cell Biol. 2014;207:549-67 pubmed publisher
    ..These data provide an important foundation for further analyses of miR-214 as a key regulator of Wnt pathway activity and stem cell functions during normal tissue homeostasis, regeneration, and aging. ..
  46. Waring P, Mullbacher A. Cell death mediated by alloreactive cytotoxic T cells via the granule exocytosis or the Fas pathway is independent of p34cdc2 kinase: Fas dependent killing of cells arrested in the cell cycle. Immunol Cell Biol. 2001;79:264-73 pubmed
    ..This lysis was accompanied by an increase in cell surface Fas expression. Olomoucine induced cell cycle arrest and expression of Fas was reversible and when cells re-entered the cell cycle, surface expression of Fas was lost. ..
  47. Wheeler L, Lents N, Baldassare J. Cyclin A-CDK activity during G1 phase impairs MCM chromatin loading and inhibits DNA synthesis in mammalian cells. Cell Cycle. 2008;7:2179-88 pubmed
    ..Cyclin A-CDK2 and cyclin A-CDK1 phosphorylate nuclear substrates during S and G(2) phases, respectfully...
  48. Pilkinton M, Sandoval R, Song J, Ness S, Colamonici O. Mip/LIN-9 regulates the expression of B-Myb and the induction of cyclin A, cyclin B, and CDK1. J Biol Chem. 2007;282:168-75 pubmed
    ..e. cyclin A, CDK1, and cyclin B)...
  49. Rhee K, Wolgemuth D. Cdk family genes are expressed not only in dividing but also in terminally differentiated mouse germ cells, suggesting their possible function during both cell division and differentiation. Dev Dyn. 1995;204:406-20 pubmed
    ..family in murine germ cell development have been examined by studying the expression of five Cdk family genes (Cdc2, Cdk2, Cdk4, Pctaire-1, and Pctaire-3) in mouse reproductive organs...
  50. Clement T, Inselman A, Goulding E, Willis W, Eddy E. Disrupting Cyclin Dependent Kinase 1 in Spermatocytes Causes Late Meiotic Arrest and Infertility in Mice. Biol Reprod. 2015;93:137 pubmed publisher
    While cyclin dependent kinase 1 (CDK1) has a critical role in controlling resumption of meiosis in oocytes, its role has not been investigated directly in spermatocytes...
  51. Hogg A, Schirm S, Nakagoshi H, Bartley P, Ishii S, Bishop J, et al. Inactivation of a c-Myb/estrogen receptor fusion protein in transformed primary cells leads to granulocyte/macrophage differentiation and down regulation of c-kit but not c-myc or cdc2. Oncogene. 1997;15:2885-98 pubmed
    ..The expression of the c-myc, c-kit, cdc2 and bcl-2 genes, which are putatively regulated by Myb, was investigated in ERMYB cells grown in the presence or ..
  52. Zhang Q, Sivakumar S, Chen Y, Gao H, Yang L, Yuan Z, et al. Ska3 Phosphorylated by Cdk1 Binds Ndc80 and Recruits Ska to Kinetochores to Promote Mitotic Progression. Curr Biol. 2017;27:1477-1484.e4 pubmed publisher
    ..Here, we show that Cdk1 phosphorylates Ska3 to promote its direct binding to the Ndc80 complex (Ndc80C), a core outer kinetochore ..
  53. Firmani L, Uliasz T, Mehlmann L. The switch from cAMP-independent to cAMP-dependent arrest of meiotic prophase is associated with coordinated GPR3 and CDK1 expression in mouse oocytes. Dev Biol. 2018;434:196-205 pubmed publisher
    ..they do not possess adequate amounts of cell cycle regulatory proteins, particularly cyclin-dependent kinase 1 (CDK1). As oocytes grow, they synthesize CDK1 and acquire the ability to mature...
  54. Trakala M, Rodríguez Acebes S, Maroto M, Symonds C, Santamaría D, Ortega S, et al. Functional reprogramming of polyploidization in megakaryocytes. Dev Cell. 2015;32:155-67 pubmed publisher
    ..of the APC/C cofactor Cdc20 results in mitotic arrest and severe thrombocytopenia, lack of the kinases Aurora-B, Cdk1, or Cdk2 does not affect megakaryocyte polyploidization or platelet levels...
  55. Sansregret L, Gallo D, Santaguida M, Leduy L, Harada R, Nepveu A. Hyperphosphorylation by cyclin B/CDK1 in mitosis resets CUX1 DNA binding clock at each cell cycle. J Biol Chem. 2010;285:32834-43 pubmed publisher
    ..We show that cyclin B/CDK1 interacts with CUX1 and phosphorylates it at multiple sites...
  56. Inaki M, Kato D, Utsugi T, Onoda F, Hanaoka F, Murakami Y. Genetic analyses using a mouse cell cycle mutant identifies magoh as a novel gene involved in Cdk regulation. Genes Cells. 2011;16:166-78 pubmed publisher
    ..We show that, in addition to ccnb1 and cks2, magoh is also a dosage suppressor of the mouse temperature-sensitive cdc2 mutant, and synthetic enhancement of the cdc2 ts phenotype by RNA interference (RNAi) of magoh is observed in a ..
  57. Kaushal N, Bansal M. Inhibition of CDC2/Cyclin B1 in response to selenium-induced oxidative stress during spermatogenesis: potential role of Cdc25c and p21. Mol Cell Biochem. 2007;298:139-50 pubmed
    ..Various cell cycle regulators control and coordinate the process of cell cycle. Because of the crucial involvement of CDC2, Cyclin B1, Cdc25c, and p21 in cell cycle regulation, the present study was aimed to investigate the possibility ..
  58. Tintignac L, Sirri V, Leibovitch M, Lecluse Y, Castedo M, Metivier D, et al. Mutant MyoD lacking Cdc2 phosphorylation sites delays M-phase entry. Mol Cell Biol. 2004;24:1809-21 pubmed
    ..the G(2)/M transition by controlling the expression of p21(Waf1/Cip1) (p21), which is believed to regulate cyclin B-Cdc2 kinase activity in G(2)...
  59. Th ng J, Wright P, Hamaguchi J, Lee M, Norbury C, Nurse P, et al. The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. Cell. 1990;63:313-24 pubmed
    ..These mutations cause the p34 protein kinase to become inactivated and degraded in FT210 cells at the restrictive temperature, 39 degrees C...
  60. Hasthorpe S, Tainton K, Peart M, Roeszler K, Bell K, Lusby P, et al. G2/M checkpoint gene expression in developing germ cells. Mol Reprod Dev. 2007;74:531-8 pubmed
    ..0001) and Cdc2 (P<0.02) mRNA...
  61. Jun D, Park H, Nordin A, Nagel J, Kim Y. Characterization of the murine cdc2 gene. Mol Cells. 1998;8:731-40 pubmed
    A 1204 bp full-length cDNA encoding murine cdc2 was isolated and used as a probe to obtain four overlapping cdc2 genomic clones which span the entire cdc2 sequence...
  62. Linares J, Amanchy R, Greis K, Diaz Meco M, Moscat J. Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell proliferation. Mol Cell Biol. 2011;31:105-17 pubmed publisher
    ..Here we show that cdk1 phosphorylates p62 in vitro and in vivo at T269 and S272, which is necessary for the maintenance of appropriate ..
  63. Yuan L, Yu W, Qu C. DNA damage-induced G2/M checkpoint in SV40 large T antigen-immortalized embryonic fibroblast cells requires SHP-2 tyrosine phosphatase. J Biol Chem. 2003;278:42812-20 pubmed
    ..These results together provide the first evidence that SHP-2 tyrosine phosphatase enhances the DNA damage G2/M checkpoint in SV40 large T antigen immortalized murine embryonic fibroblast cells. ..
  64. Morice W, Wiederrecht G, Brunn G, Siekierka J, Abraham R. Rapamycin inhibition of interleukin-2-dependent p33cdk2 and p34cdc2 kinase activation in T lymphocytes. J Biol Chem. 1993;268:22737-45 pubmed
    ..The inhibitory effects of RAP on the activation of cyclin E- and cyclin A-associated cyclin-dependent kinases suggest that one or both events participate in the regulation of T cell entry into S-phase. ..
  65. Diril M, Bisteau X, Kitagawa M, Caldez M, Wee S, Gunaratne J, et al. Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet. 2016;12:e1006310 pubmed publisher
    The Greatwall kinase/Mastl is an essential gene that indirectly inhibits the phosphatase activity toward mitotic Cdk1 substrates...
  66. Sette C, Barchi M, Bianchini A, Conti M, Rossi P, Geremia R. Activation of the mitogen-activated protein kinase ERK1 during meiotic progression of mouse pachytene spermatocytes. J Biol Chem. 1999;274:33571-9 pubmed
    ..condensation in cultured pachytene spermatocytes and an increase in maturation promoting factor (cyclin B1/cdc2 kinase) activity, as evaluated by H1 phosphorylative activity in anti-cyclin B1 immunoprecipitates...
  67. Risley M, Clowes C, Yu M, Mitchell K, Hentges K. The Mediator complex protein Med31 is required for embryonic growth and cell proliferation during mammalian development. Dev Biol. 2010;342:146-56 pubmed publisher
    ..from mutant embryos have a severe proliferation defect, as well as reduced levels of the cell cycle protein Cdc2. Med31 mutants have normal limb bud patterning but defective or delayed chondrogenesis due to a lack of Sox9 and ..
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