Gene Symbol: Cdc25b
Description: cell division cycle 25B
Alias: AI604853, M-phase inducer phosphatase 2, dual specificity phosphatase Cdc25B
Species: mouse
Products:     Cdc25b

Top Publications

  1. Cui C, Zhao H, Zhang Z, Zong Z, Feng C, Zhang Y, et al. CDC25B acts as a potential target of PRKACA in fertilized mouse eggs. Biol Reprod. 2008;79:991-8 pubmed publisher
    ..In this study, we investigated the role of the PRKACA/CDC25B pathway on the early development of mouse fertilized eggs...
  2. Meng J, Cui C, Liu Y, Jin M, Wu D, Liu C, et al. The role of 14-3-3? interaction with phosphorylated Cdc25B at its Ser321 in the release of the mouse oocyte from prophase I arrest. PLoS ONE. 2013;8:e53633 pubmed publisher
    The protein kinase A (PKA)/Cdc25B pathway plays a critical role in maintaining meiotic arrest in mouse oocytes. However, the molecular mechanism underlying this interchange is not known...
  3. Zhang Y, Zhang Z, Xu X, Li X, Yu M, Yu A, et al. Protein kinase A modulates Cdc25B activity during meiotic resumption of mouse oocytes. Dev Dyn. 2008;237:3777-86 pubmed publisher
    ..In this study, we investigated the regulation of meiotic resumption by PKA/Cdc25B pathway in mouse oocytes...
  4. Lincoln A, Wickramasinghe D, Stein P, Schultz R, Palko M, De Miguel M, et al. Cdc25b phosphatase is required for resumption of meiosis during oocyte maturation. Nat Genet. 2002;30:446-9 pubmed
    ..The dual specificity phosphatases Cdc25a, Cdc25b and Cdc25c are activators of cyclin-dependent kinases; consequently, they are postulated to regulate cell-cycle ..
  5. Oh J, Han S, Conti M. Wee1B, Myt1, and Cdc25 function in distinct compartments of the mouse oocyte to control meiotic resumption. J Cell Biol. 2010;188:199-207 pubmed publisher
    ..Shortly before GV breakdown (GVBD), Cdc25B is translocated from the cytoplasm to the nucleus, whereas Wee1B is exported from the nucleus to the cytoplasm...
  6. Pirino G, Wescott M, Donovan P. Protein kinase A regulates resumption of meiosis by phosphorylation of Cdc25B in mammalian oocytes. Cell Cycle. 2009;8:665-70 pubmed
    ..However, in mice, studies suggest that Cdc25B is the phosphatase essential for GVBD and, therefore, the likely target of PKA activity...
  7. Ferguson A, White L, Donovan P, Piwnica Worms H. Normal cell cycle and checkpoint responses in mice and cells lacking Cdc25B and Cdc25C protein phosphatases. Mol Cell Biol. 2005;25:2853-60 pubmed
    ..In humans and rodents, there are three Cdc25 family members--denoted Cdc25A, Cdc25B, and Cdc25C--that can be distinguished based on their subcellular compartmentalizations, their abundances and/or ..
  8. Xiao J, Liu C, Hou J, Cui C, Wu D, Fan H, et al. Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs. J Biol Chem. 2011;286:10356-66 pubmed publisher a negative regulator of M phase promoting factor (MPF) by phosphorylating cell division cycle 25 homolog B (Cdc25B) in mammals. However, the molecular mechanism remains unclear...
  9. Solc P, Saskova A, Baran V, Kubelka M, Schultz R, Motlik J. CDC25A phosphatase controls meiosis I progression in mouse oocytes. Dev Biol. 2008;317:260-9 pubmed publisher
    ..Although CDC25C is not essential for either mitotic or meiotic cell cycle regulation, CDC25B is essential for CDK1 activation during resumption of meiosis...

More Information


  1. Lee G, White L, Hurov K, Stappenbeck T, Piwnica Worms H. Response of small intestinal epithelial cells to acute disruption of cell division through CDC25 deletion. Proc Natl Acad Sci U S A. 2009;106:4701-6 pubmed publisher
    ..CDC25A and CDC25B are frequently overproduced in human cancers...
  2. Cui C, Tang H, Yu B. [Cdc25B overexpression reverses two-cell block in mouse embryos]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008;24:99-103 pubmed
    To explore the effect of Cdc25B overexpression on the development of mouse two-cell embryos. The pBSK-Cdc25B was in vitro transcribed into 5'-capped mRNA for microinjection by using mMESSAGE mMACHINE kit...
  3. Wu S, Wolgemuth D. The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis. Dev Biol. 1995;170:195-206 pubmed
    ..performed to determine the patterns of expression of Cdc25C compared to those of a second Cdc25 family member, Cdc25B. Murine Cdc25C transcripts of 2...
  4. Uchida S, Watanabe N, Kudo Y, Yoshioka K, Matsunaga T, Ishizaka Y, et al. SCF?(TrCP) mediates stress-activated MAPK-induced Cdc25B degradation. J Cell Sci. 2011;124:2816-25 pubmed publisher
    ..Previously, we showed that Cdc25B is primarily degraded by cellular stresses that activate stress-activated MAPKs, such as Jun NH(2)-terminal kinase ..
  5. Gruber R, Zhou Z, Sukchev M, Joerss T, Frappart P, Wang Z. MCPH1 regulates the neuroprogenitor division mode by coupling the centrosomal cycle with mitotic entry through the Chk1-Cdc25 pathway. Nat Cell Biol. 2011;13:1325-34 pubmed publisher
    ..Silencing Cdc25b, a centrosome substrate of Chk1, corrects MCPH1-deficiency-induced spindle misalignment and rescues the premature ..
  6. Zander M, Burns S, Yang G, Kaplan D, Miller F. Snail coordinately regulates downstream pathways to control multiple aspects of mammalian neural precursor development. J Neurosci. 2014;34:5164-75 pubmed publisher
    ..Second, we show that the cell cycle phosphatase Cdc25b is regulated by Snail in radial precursors and that Cdc25b coexpression is sufficient to rescue the decreased ..
  7. Cui C, Ren X, Liu D, Deng X, Qin X, Zhao X, et al. 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B. BMC Dev Biol. 2014;14:33 pubmed publisher
    ..Here, we showed that 14-3-3? interacts and cooperates with CDC25B phosphorylated at Ser321 regulating G2/M transition of mitotic progress of fertilized mouse eggs...
  8. Umansky K, Gruenbaum Cohen Y, Tsoory M, Feldmesser E, Goldenberg D, Brenner O, et al. Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration. PLoS Genet. 2015;11:e1005457 pubmed publisher
    ..The data provide unique insights into the transcriptional program driving muscle regeneration and implicate Runx1 as an important participant in the pathology of muscle wasting diseases. ..
  9. Krupczak Hollis K, Wang X, Kalinichenko V, Gusarova G, Wang I, Dennewitz M, et al. The mouse Forkhead Box m1 transcription factor is essential for hepatoblast mitosis and development of intrahepatic bile ducts and vessels during liver morphogenesis. Dev Biol. 2004;276:74-88 pubmed
  10. Wu X, Gu X, Han X, Du A, Jiang Y, Zhang X, et al. A novel function for Foxm1 in interkinetic nuclear migration in the developing telencephalon and anxiety-related behavior. J Neurosci. 2014;34:1510-22 pubmed publisher
    ..results in abnormal cell cycle progression, leading to impaired INM through the downregulation of Cyclin b1 and Cdc25b. The impairment of INM disturbs the synchronization of apical progenitors (APs) and promotes the transition from ..
  11. Lee G, Origanti S, White L, Sun J, Stappenbeck T, Piwnica Worms H. Contributions made by CDC25 phosphatases to proliferation of intestinal epithelial stem and progenitor cells. PLoS ONE. 2011;6:e15561 pubmed publisher
    ..Finally, we demonstrate that CDC25A and CDC25B but not CDC25C compensate for each other to maintain the proliferative capacity of intestinal epithelial stem and ..
  12. Cui C, Zang T, Cao Y, Qin X, Zhang X. CDC25B is involved in the centrosomal microtubule nucleation in two-cell stage mouse embryos. Dev Growth Differ. 2016;58:714-726 pubmed publisher
    b>CDC25B has been demonstrated to activate the complex of CDK1/Cyclin B and trigger mitosis...
  13. Schmidt E, Bondareva A, Radke J, Capecchi M. Fundamental cellular processes do not require vertebrate-specific sequences within the TATA-binding protein. J Biol Chem. 2003;278:6168-74 pubmed
    ..or in expression of the proliferative markers dihydrofolate reductase (DHFR) mRNA (S phase-specific) and cdc25B mRNA (G(2)-specific)...
  14. Castro D, Skowronska Krawczyk D, Armant O, Donaldson I, Parras C, Hunt C, et al. Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif. Dev Cell. 2006;11:831-44 pubmed
    ..We thus propose that Mash1 synergizes with Brn factors to regulate multiple steps of neurogenesis. ..
  15. Kim J, Singh A, Takata Y, Lin K, Shen J, Lu Y, et al. LSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice. Nat Commun. 2015;6:10116 pubmed publisher
    ..Mechanistically, upregulation of CDC25B, a phosphatase that activates CDK1, is responsible for precocious meiotic resumption and also contributes to ..
  16. Marangos P, Carroll J. Oocytes progress beyond prophase in the presence of DNA damage. Curr Biol. 2012;22:989-94 pubmed publisher
    ..cyclin B-CDK1 through destruction of Cdc25A phosphatase, oocytes utilize an inhibitory phosphorylation of Cdc25B. We conclude that oocytes are the only nontransformed cells that fail to launch a robust G2 phase DNA damage ..
  17. Park Y, Jeon Y, Kim I. Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B. Biochim Biophys Acta. 2012;1823:2217-26 pubmed publisher
    ..We also found that inactive Cdk1 is caused by the sustained inactivation of CDC25B, which removes the inhibitory phosphate from Cdk1...
  18. Lock L, Wickramasinghe D, Ernst M, Gilbert D, Copeland N, Jenkins N, et al. The Cdc25 genes map to mouse chromosomes 2, 9, and 18. Mamm Genome. 1996;7:771-2 pubmed
  19. Kakizuka A, Sebastian B, Borgmeyer U, Hermans Borgmeyer I, Bolado J, Hunter T, et al. A mouse cdc25 homolog is differentially and developmentally expressed. Genes Dev. 1992;6:578-90 pubmed
    ..We suggest that the expression and accumulation of the cdc25 mitotic inducer may play a critical role in the regulation of mouse development. ..
  20. Xiao J, Liu C, Sun X, Yu B. [PKA-regulated phosphorylation status of S149 and S321 sites of CDC25B inhibits mitosis of fertilized mouse eggs]. Sheng Li Xue Bao. 2012;64:33-40 pubmed the role of PKA in regulating the phosphorylation status of S149 and S321 sites of cell division cycle 25B (CDC25B) on one-cell stage fertilized mouse eggs, pBSK-CDC25B-WT, pBSK-CDC25B-S149A, pBSK-CDC25B-S321A and pBSK-CDC25B-..
  21. Wickramasinghe D, Becker S, Ernst M, Resnick J, Centanni J, Tessarollo L, et al. Two CDC25 homologues are differentially expressed during mouse development. Development. 1995;121:2047-56 pubmed
    ..of the mouse genes with human CDC25 genes reveal that they are most likely the mouse homologs of human CDC25A and CDC25B respectively...
  22. Dakubo G, Beug S, Mazerolle C, Thurig S, Wang Y, Wallace V. Control of glial precursor cell development in the mouse optic nerve by sonic hedgehog from retinal ganglion cells. Brain Res. 2008;1228:27-42 pubmed publisher
    ..mutant mice; however, the expression of Hedgehog (Hh) target genes, Gli1 and Ptch1 and cell cycle genes, Ccnd1 and Cdc25b in the optic nerves were downregulated. Astrocyte proliferation was markedly reduced...
  23. Zhao X, Feng C, Yu D, Deng X, Wu D, Jin M, et al. Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest. Dev Dyn. 2015;244:110-21 pubmed publisher
    ..It is generally believed that CDC25B acts as a "starter phosphatase" of mitosis...
  24. Song Y, Li Z, Wang B, Xiao J, Wang X, Huang J. Phospho-Cdc25 correlates with activating G2/M checkpoint in mouse zygotes fertilized with hydrogen peroxide-treated mouse sperm. Mol Cell Biochem. 2014;396:41-8 pubmed publisher
    ..Immunofluorescence staining revealed Phospho-Cdc25C (Ser216) and Phospho-Cdc25B (Ser323) in or surrounding a single pronucleus, following insemination with H2O2-treated sperm...
  25. Solc P, Baran V, Mayer A, Bohmova T, Panenkova Havlova G, Saskova A, et al. Aurora kinase A drives MTOC biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo. Biol Reprod. 2012;87:85 pubmed publisher
    ..AURKA activation is biphasic with the initial activation not requiring CDC25B-CDK1 activity, whereas full activation, which is essential for the increase in MTOCs number, depends on CDK1 ..
  26. Gunn T, Miller K, He L, Hyman R, Davis R, Azarani A, et al. The mouse mahogany locus encodes a transmembrane form of human attractin. Nature. 1999;398:152-6 pubmed
    ..These observations provide new insight into the regulation of energy metabolism and indicate a molecular basis for crosstalk between melanocortin-receptor signalling and immune function. ..
  27. Su Y, Sugiura K, Sun F, Pendola J, Cox G, Handel M, et al. MARF1 regulates essential oogenic processes in mice. Science. 2012;335:1496-9 pubmed publisher
    ..Therefore MARF1, by suppressing levels of specific transcripts, is an essential regulator of important oogenic processes leading to female fertility and the development of healthy offspring. ..
  28. Yao Y, Slosberg E, Wang L, Hibshoosh H, Zhang Y, Xing W, et al. Increased susceptibility to carcinogen-induced mammary tumors in MMTV-Cdc25B transgenic mice. Oncogene. 1999;18:5159-66 pubmed
    ..To further assess the oncogenic capacity of Cdc25B in vivo, we have generated transgenic mice that overexpress Cdc25B in the mammary epithelium, driven by the MMTV - ..
  29. Chang K, Vincent F, Shah K. Deregulated Cdk5 triggers aberrant activation of cell cycle kinases and phosphatases inducing neuronal death. J Cell Sci. 2012;125:5124-37 pubmed publisher
    ..Using a chemical genetic approach, we identified Cdc25A, Cdc25B and Cdc25C as direct Cdk5 substrates in mouse brain lysates...
  30. Benazeraf B, Chen Q, Peco E, Lobjois V, Medevielle F, Ducommun B, et al. Identification of an unexpected link between the Shh pathway and a G2/M regulator, the phosphatase CDC25B. Dev Biol. 2006;294:133-47 pubmed
    ..Here, we show that an actor in the control of entry into mitosis, the phosphatase CDC25B, is transcriptionally upregulated by the Shh/Gli pathway...
  31. Ramakrishna S, Kim I, Petrovic V, Malin D, Wang I, Kalin T, et al. Myocardium defects and ventricular hypoplasia in mice homozygous null for the Forkhead Box M1 transcription factor. Dev Dyn. 2007;236:1000-13 pubmed
    ..Proliferation defects in Foxm1 -/- hearts were associated with a reduced expression of Cdk1-activator Cdc25B phosphatase and NFATc3 transcription factor, and with abnormal nuclear accumulation of the Cdk-inhibitor p21(Cip1) ..
  32. Cha H, Wang X, Li H, Fornace A. A functional role for p38 MAPK in modulating mitotic transit in the absence of stress. J Biol Chem. 2007;282:22984-92 pubmed
    ..Consistent with our previous findings, Cdc25B was phosphorylated on serine 309 at the centrosome during G(2)/M when p38 was active at this site; Cdc25B ..
  33. Kang H, Hwang S, Park Y, Oh J. Cdc25B phosphatase participates in maintaining metaphase II arrest in mouse oocytes. Mol Cells. 2013;35:514-8 pubmed publisher
    b>Cdc25B is an essential regulator for meiotic resumption in mouse oocytes. However, the role of this phosphatase during the later stage of the meiotic cell cycle is not known...