Canx

Summary

Gene Symbol: Canx
Description: calnexin
Alias: 1110069N15Rik, AI988026, Cnx, D11Ertd153e
Species: mouse

Top Publications

  1. ncbi Beyond lectins: the calnexin/calreticulin chaperone system of the endoplasmic reticulum
    David B Williams
    Department of Biochemistry and Immunology, University of Toronto, Toronto, Ontario, Canada, M5S 1A8
    J Cell Sci 119:615-23. 2006
  2. pmc Adaptation to ER stress is mediated by differential stabilities of pro-survival and pro-apoptotic mRNAs and proteins
    D Thomas Rutkowski
    Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor, Michigan, USA
    PLoS Biol 4:e374. 2006
  3. pmc GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development
    Shengzhan Luo
    Department of Biochemistry and Molecular Biology and the USC Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, Los Angeles, CA 90089 9176, USA
    Mol Cell Biol 26:5688-97. 2006
  4. pmc Calnexin deficiency leads to dysmyelination
    Allison Kraus
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 285:18928-38. 2010
  5. ncbi Cleavage of calnexin caused by apoptotic stimuli: implication for the regulation of apoptosis
    Takenori Takizawa
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Kasugai 480 0392, Japan
    J Biochem 136:399-405. 2004
  6. ncbi Calnexin: a membrane-bound chaperone of the endoplasmic reticulum
    J J Bergeron
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    Trends Biochem Sci 19:124-8. 1994
  7. pmc Early postnatal death and motor disorders in mice congenitally deficient in calnexin expression
    Angela Denzel
    Imperial Cancer Research Fund, Academic Department of Histopathology, Bart s and the Queen Mary s School of Medicine and Dentistry, Whitechapel, United Kingdom
    Mol Cell Biol 22:7398-404. 2002
  8. ncbi Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5
    L W Tjoelker
    ICOS Corporation, Bothell, Washington 98021
    Biochemistry 33:3229-36. 1994
  9. pmc Endoplasmic reticulum stress in the absence of calnexin
    Helen Coe
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    Cell Stress Chaperones 13:497-507. 2008
  10. ncbi Contrasting functions of calreticulin and calnexin in glycoprotein folding and ER quality control
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Mol Cell 13:125-35. 2004

Research Grants

  1. Cancer cell adaptation to metabolic stress
    Craig Thompson; Fiscal Year: 2007
  2. Apoptosis Control by Bcl-2 Family Members
    Craig Thompson; Fiscal Year: 2009
  3. Lymphocyte Survival: Implications for lymphomagenesis
    Craig Thompson; Fiscal Year: 2009
  4. Apoptosis Control by Bcl-2 Family Members
    Craig B Thompson; Fiscal Year: 2010
  5. Lymphocyte Survival: Implications for lymphomagenesis
    Craig B Thompson; Fiscal Year: 2010
  6. Cancer cell survival during factor deprivation
    Craig B Thompson; Fiscal Year: 2010
  7. Cancer cell survival during factor deprivation
    Craig Thompson; Fiscal Year: 2007
  8. Apoptosis Control by Proapoptotic Bcl-2 Family Members
    Craig Thompson; Fiscal Year: 2007
  9. Apoptosis Control by Proapoptotic Bcl-2 Family Members
    Craig Thompson; Fiscal Year: 2003
  10. Lymphocyte Survival--Implications for Lymphomagenesis
    Craig Thompson; Fiscal Year: 2005

Scientific Experts

  • Joanna Jung
  • Clifton M Ramsdell
  • Wuyi Kong
  • David B Williams
  • Xiaoping Zhu
  • Peter van Endert
  • M Molinari
  • Eileithyia Swanton
  • Takenori Takizawa
  • Craig Thompson
  • Huiping Zhu
  • Angela Denzel
  • Marek Michalak
  • Jody Groenendyk
  • Changhui Mao
  • Jingsong Cao
  • Randal J Kaufman
  • Sven Lang
  • Hao Dong Li
  • Fan Yan Wei
  • Cecil Han
  • Tomohiro Kumada
  • Allison Kraus
  • David C Silvestre
  • Haowei Song
  • Todd Hryciw
  • Florian Mertes
  • Karen Bedard
  • Yinan Zhang
  • Xin Wang
  • Sadani N Cooray
  • Tsukasa Okiyoneda
  • Paula W Lampton
  • Helen Coe
  • Amy S Lee
  • Maria Kosmaoglou
  • Vladimir M Korkhov
  • Michael A Kiebish
  • Xiaochu Zhang
  • Min Zhang
  • Belén Prados
  • Jing Zhao
  • Tatiana Soldà
  • Miao Zhang
  • D Thomas Rutkowski
  • Elisabeth Raschperger
  • Shengzhan Luo
  • Annica Pontén
  • Jovana Maric
  • Costin I Popescu
  • Kazuhide Watanabe
  • Paul Burn
  • Cary Esselens
  • Yuguang Shi
  • Alexander Rouvinski
  • H Wang
  • Fusheng Chen
  • Rai Knee
  • Wei Xing Zong
  • K M Dickson
  • Takayuki Tsukuba
  • Ludwig Edelmann
  • Richard Zimmermann
  • Birgit Kreutzer
  • E Romiti
  • Elmar Krause
  • Markus Greiner
  • Jens Rettig
  • Jorg Tatzelt
  • Stefan Schorr
  • Carolin Jalal
  • Johanna Dudek
  • Julia Benedix
  • Sorin V Fedeles
  • Stefan Somlo
  • B Teubner
  • A N Hume
  • Sarah Hassdenteufel
  • Claudia Schirra
  • Nico Schäuble
  • Mohamed Atta
  • Boyeon Lee
  • Jun Tae Kwon
  • Atsushi Fujimura
  • Sayaka Watanabe
  • Satoshi Kimura
  • Monika Dabrowska
  • Hideki Matsui
  • Sora Jin
  • C A Kozak

Detail Information

Publications72

  1. ncbi Beyond lectins: the calnexin/calreticulin chaperone system of the endoplasmic reticulum
    David B Williams
    Department of Biochemistry and Immunology, University of Toronto, Toronto, Ontario, Canada, M5S 1A8
    J Cell Sci 119:615-23. 2006
    ..Furthermore, there is clear evidence that ERp57 participates in glycoprotein biogenesis either alone or in tandem with calnexin and calreticulin...
  2. pmc Adaptation to ER stress is mediated by differential stabilities of pro-survival and pro-apoptotic mRNAs and proteins
    D Thomas Rutkowski
    Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor, Michigan, USA
    PLoS Biol 4:e374. 2006
    ..It underscores the contribution of posttranscriptional and posttranslational mechanisms in influencing this outcome...
  3. pmc GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development
    Shengzhan Luo
    Department of Biochemistry and Molecular Biology and the USC Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, Los Angeles, CA 90089 9176, USA
    Mol Cell Biol 26:5688-97. 2006
    ..These findings provide the first evidence that GRP78 is essential for embryonic cell growth and pluripotent cell survival...
  4. pmc Calnexin deficiency leads to dysmyelination
    Allison Kraus
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 285:18928-38. 2010
    ..We have generated calnexin gene-deficient mice (cnx(-/-)) and showed that calnexin deficiency leads to myelinopathy...
  5. ncbi Cleavage of calnexin caused by apoptotic stimuli: implication for the regulation of apoptosis
    Takenori Takizawa
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Kasugai 480 0392, Japan
    J Biochem 136:399-405. 2004
    ..These results collectively suggest that caspase-3 or caspase-7 cleaves calnexin, whose cleaved product leads to the attenuation of apoptosis...
  6. ncbi Calnexin: a membrane-bound chaperone of the endoplasmic reticulum
    J J Bergeron
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    Trends Biochem Sci 19:124-8. 1994
    ..One of its demonstrated functions is the retention of incorrectly or incompletely folded proteins, suggesting that calnexin is a component of the quality control system of the endoplasmic reticulum...
  7. pmc Early postnatal death and motor disorders in mice congenitally deficient in calnexin expression
    Angela Denzel
    Imperial Cancer Research Fund, Academic Department of Histopathology, Bart s and the Queen Mary s School of Medicine and Dentistry, Whitechapel, United Kingdom
    Mol Cell Biol 22:7398-404. 2002
    ..Thus, the critical contribution of calnexin to mammalian physiology is tissue specific...
  8. ncbi Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5
    L W Tjoelker
    ICOS Corporation, Bothell, Washington 98021
    Biochemistry 33:3229-36. 1994
    ..The gene for human calnexin is located on the distal end of the long arm of human chromosome 5, at 5q35...
  9. pmc Endoplasmic reticulum stress in the absence of calnexin
    Helen Coe
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    Cell Stress Chaperones 13:497-507. 2008
    ..This indicates that cnx (-/-) cells have increased constitutively active UPR...
  10. ncbi Contrasting functions of calreticulin and calnexin in glycoprotein folding and ER quality control
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Mol Cell 13:125-35. 2004
    ..Although not fully interchangeable during assistance of glycoprotein folding, calreticulin and calnexin may work, independently, as efficient and crucial factors for retention in the ER of nonnative polypeptides...
  11. ncbi Chaperone interactions of the metalloproteinase meprin A in the secretory or proteasomal-degradative pathway
    Takayuki Tsukuba
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 0850, USA
    Arch Biochem Biophys 397:191-8. 2002
    ....
  12. doi Cell surface targeting of myelin oligodendrocyte glycoprotein (MOG) in the absence of endoplasmic reticulum molecular chaperones
    Joanna Jung
    Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Canada T6G 2H7
    Biochim Biophys Acta 1813:1105-10. 2011
    ..Importantly, we show that cell surface targeting of MOG is not disrupted in the absence of the endoplasmic reticulum chaperones. This article is part of a special issue entitled: 11th European Symposium on Calcium...
  13. pmc Bax and Bak can localize to the endoplasmic reticulum to initiate apoptosis
    Wei Xing Zong
    Department of Cancer Biology, Abramson Cancer Center, 421 Curie Blvd, BRB II III, 445, Philadelphia, PA 19104 6160, USA
    J Cell Biol 162:59-69. 2003
    ..These findings demonstrate that in addition to their functions at mitochondria, Bax and Bak also localize to the ER and function to initiate a parallel pathway of caspase activation and apoptosis...
  14. pmc Rab27a regulates the peripheral distribution of melanosomes in melanocytes
    A N Hume
    Cell and Molecular Biology Section, Division of Biomedical Sciences, London, United Kingdom
    J Cell Biol 152:795-808. 2001
    ..These results, together with recent data implicating myosinVa in the peripheral capture of melanosomes, suggest that Rab27a is necessary for the recruitment of myosinVa, so allowing the peripheral retention of melanosomes in melanocytes...
  15. pmc Deficit of tRNA(Lys) modification by Cdkal1 causes the development of type 2 diabetes in mice
    Fan Yan Wei
    Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan
    J Clin Invest 121:3598-608. 2011
    ..These findings suggest that glucose-stimulated translation of proinsulin may require fully modified tRNA(Lys)(UUU), which could potentially explain the molecular pathogenesis of T2D in patients carrying cdkal1 risk alleles...
  16. doi Different effects of Sec61α, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells
    Sven Lang
    Medical Biochemistry and Molecular Biology, Saarland University, Homburg, Germany
    J Cell Sci 125:1958-69. 2012
    ..By contrast, silencing the SEC62 gene inhibited only post-translational transport of a signal-peptide-containing precursor protein...
  17. doi Peptide-based interactions with calnexin target misassembled membrane proteins into endoplasmic reticulum-derived multilamellar bodies
    Vladimir M Korkhov
    Institute of Pharmacology, Center of Biomolecular Medicine and Pharmacology, Medical University Vienna, Waehringer Strasse 13a, A 1090 Vienna, Austria
    J Mol Biol 378:337-52. 2008
    ..Our findings are consistent with a model in which the transmembrane segment of calnexin participates in chaperoning the inter- and intramolecular arrangement of hydrophobic segment in oligomeric proteins...
  18. doi Role of calnexin in the ER quality control and productive folding of CFTR; differential effect of calnexin knockout on wild-type and DeltaF508 CFTR
    Tsukasa Okiyoneda
    Department of Molecular Medicine, Graduate School of Medical and Pharmaceutical Sciences, Global COE Cell Fate Regulation Research and Education Unit, Kumamoto University, Kumamoto 862 0973, Japan
    Biochim Biophys Acta 1783:1585-94. 2008
    ..Here, we show that calnexin (CNX) is not necessary for the ER retention of DeltaF508 CFTR...
  19. pmc Lipidomic analysis and electron transport chain activities in C57BL/6J mouse brain mitochondria
    Michael A Kiebish
    Biology Department, Boston College, Chestnut Hill, Massachusetts 02467, USA
    J Neurochem 106:299-312. 2008
    ..NS and Syn mitochondrial lipidomic heterogeneity could influence energy metabolism, which may contribute to metabolic compartmentation of the brain...
  20. pmc Differentially expressed genes in embryonic cardiac tissues of mice lacking Folr1 gene activity
    Huiping Zhu
    Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, Texas 77030, USA
    BMC Dev Biol 7:128. 2007
    ..Defective function of the Folr1 (also known as Folbp1; homologue of human FRalpha) gene in mice results in inadequate transport, accumulation, or metabolism of folate during cardiovascular morphogenesis...
  21. ncbi Calumin, a novel Ca2+-binding transmembrane protein on the endoplasmic reticulum
    Miao Zhang
    Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Cell Calcium 42:83-90. 2007
    ..These observations suggest that calumin plays an essential role in ER Ca2+ handling and is also implicated in signaling from the ER, which is closely associated with cell-fate decision...
  22. ncbi Altered vitamin A homeostasis and increased size and adiposity in the rdh1-null mouse
    Min Zhang
    Nutritional Science and Toxicology, University of California, Berkeley, CA 94720 3104, USA
    FASEB J 21:2886-96. 2007
    ....
  23. pmc Expression of the murine Pomt1 gene in both the developing brain and adult muscle tissues and its relationship with clinical aspects of Walker-Warburg syndrome
    Belén Prados
    Department of Biochemistry, Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Facultad de Medicina, Universidad Autonoma de Madrid, 28029 Madrid, Spain
    Am J Pathol 170:1659-68. 2007
    ..This expression pattern in the testes could also be related to the gonadal anomalies observed in some patients with WWS...
  24. pmc The role of tapasin in MHC class I protein trafficking in embryos and T cells
    Paula W Lampton
    Department of Biology, 134 Mugar Hall, Northeastern University, Boston, MA 02115, USA
    J Reprod Immunol 78:28-39. 2008
    ..Identification of the molecules involved in regulation of MHC class I protein expression in early embryos is an important first step in gaining insight into mechanisms of escape of embryos from destruction by the maternal immune system...
  25. ncbi Substrate-specific requirements for UGT1-dependent release from calnexin
    Tatiana Soldà
    Institute for Research in Biomedicine, Bellinzona, Switzerland
    Mol Cell 27:238-49. 2007
    ..For other proteins, UGT1 deletion substantially delays release from calnexin, unexpectedly showing that UGT1 activity might be required for a structural maturation needed for substrate dissociation from calnexin and export from the ER...
  26. doi Cloning of mouse ojoplano, a reticular cytoplasmic protein expressed during embryonic development
    Florian Mertes
    Max Planck Institute for Molecular Genetics, Berlin, Germany
    Gene Expr Patterns 9:562-7. 2009
    ..Opo shows homology to human transcripts linked to a hereditary craniofacial malformation, orofacial cleft 1 (OFC1). The expression of mouse Opo in neural crest derivatives and skull elements further supports this link...
  27. pmc Calnexin is not essential for mammalian rod opsin biogenesis
    Maria Kosmaoglou
    UCL Institute of Ophthalmology, London, UK
    Mol Vis 14:2466-74. 2008
    ..A recent report has shown that Drosophila rhodopsin Rh1 requires calnexin (Cnx) for its maturation and correct localization to R1-6 rhabdomeres...
  28. pmc Enhanced clathrin-dependent endocytosis in the absence of calnexin
    Hao Dong Li
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 6:e21678. 2011
    ..The endoplasmic reticulum luminal domain of calnexin is responsible for lectin-like activity and interaction with nascent polypeptide chains. The role of the C-terminal, cytoplasmic portion of calnexin is not clear...
  29. doi Identification of heat shock protein 5, calnexin and integral membrane protein 2B as Adam7-interacting membrane proteins in mouse sperm
    Cecil Han
    School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea
    J Cell Physiol 226:1186-95. 2011
    ..This analysis revealed that Adam7 forms complexes with calnexin (Canx), heat shock protein 5 (Hspa5), and integral membrane protein 2B (Itm2b)...
  30. pmc Effects of endoplasmic reticulum stress on group VIA phospholipase A2 in beta cells include tyrosine phosphorylation and increased association with calnexin
    Haowei Song
    Mass Spectrometry Resource, Division of Endocrinology, Metabolism, and Lipid Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 285:33843-57. 2010
    ..Similar results were obtained with mouse pancreatic islets...
  31. doi Ttyh1, a Ca(2+)-binding protein localized to the endoplasmic reticulum, is required for early embryonic development
    Tomohiro Kumada
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Dev Dyn 239:2233-45. 2010
    ..Our findings indicate that Ttyh1 plays an indispensable role during mitosis in early embryogenesis, possibly by maintaining Ca(2+) homeostasis in the ER...
  32. pmc Targeted mutation of the mouse Grp94 gene disrupts development and perturbs endoplasmic reticulum stress signaling
    Changhui Mao
    Department of Biochemistry and Molecular Biology, University of Southern California Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, California, USA
    PLoS ONE 5:e10852. 2010
    ..Furthermore, from analysis of microarray database and immunohistochemical staining, we present predictions where GRP94 may play an important role in specific adult organ homeostasis and function...
  33. ncbi Involvement of the chaperone protein calnexin and the acetylcholine receptor beta-subunit in the assembly and cell surface expression of the receptor
    S H Keller
    Department of Pharmacology 0636, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 271:22871-7. 1996
    ..Thus, calnexin appears to associate with the individual nascent subunits, thereby facilitating their assembly into the mature pentameric receptor...
  34. doi Mutational analysis of calnexin
    Jody Groenendyk
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    Biochim Biophys Acta 1808:1435-40. 2011
    ....
  35. pmc Growth of peripheral and central nervous system tumors is supported by cytoplasmic c-Fos in humans and mice
    David C Silvestre
    Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, CIQUIBIC, Universidad Nacional de Cordoba, Cordoba, Argentina
    PLoS ONE 5:e9544. 2010
    ..Herein we examined phospholipid synthesis status in brain tumors from human patients and from NPcis mice, an animal model of the human disease Neurofibromatosis Type 1 (NF1)...
  36. doi Cyclophilin C-associated protein/Mac-2 binding protein colocalizes with calnexin and regulates the expression of tissue transglutaminase
    Wuyi Kong
    Children s Surgical Research Program, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    J Cell Physiol 223:151-7. 2010
    ..Our data give novel evidence that CyCAP regulates the post-translational modification of tTG through its colocalization with calnexin in ER...
  37. doi The fibroblast growth factor receptor substrate 3 adapter is a developmentally regulated microtubule-associated protein expressed in migrating and differentiated neurons
    Todd Hryciw
    Molecular Brain Research Group, Robarts Research Institute, London, Ontario, Canada
    J Neurochem 112:924-39. 2010
    ..Collectively, these data suggest that neuronal Frs3 functions as a novel microtubule binding protein and they provide the first biochemical evidence that neuronal Frs3 is functionally distinct from Frs2/Frs2alpha...
  38. doi Accessory proteins are vital for the functional expression of certain G protein-coupled receptors
    Sadani N Cooray
    Centre for Endocrinology, William Harvey Research Institute, Barts and The London, London, UK
    Mol Cell Endocrinol 300:17-24. 2009
    ..Understanding their roles in GPCR expression would not only give us an insight into these receptors from a cell biological point of view but may also potentially lead to the development of novel therapeutics...
  39. pmc ERp57 does not require interactions with calnexin and calreticulin to promote assembly of class I histocompatibility molecules, and it enhances peptide loading independently of its redox activity
    Yinan Zhang
    Departments of Biochemistry and Immunology, University of Toronto, Toronto M5S 1A8, Canada
    J Biol Chem 284:10160-73. 2009
    ..Thus, ERp57 appears to play a structural rather than catalytic role within the peptide loading complex...
  40. pmc Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice
    Xin Wang
    Laboratory of Cell and Developmental Signaling, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    J Cell Biol 184:143-58. 2009
    ..Thus, global compromise of ER and mitochondrial integrity caused by ceramide accumulation in CERT mutant mice primarily affects organogenesis rather than causing cell death via apoptotic pathways...
  41. doi Specialization of endoplasmic reticulum chaperones for the folding and function of myelin glycoproteins P0 and PMP22
    Joanna Jung
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    FASEB J 25:3929-37. 2011
    ....
  42. ncbi The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis
    Elisabeth Raschperger
    Ludwig Institute for Cancer Research, Nobels v 3, SE 171 77 Stockholm, Sweden
    Exp Cell Res 312:1566-80. 2006
    ..We conclude that CAR is localized to epithelial tight junctions in vivo where it may play a role in the regulation of epithelial permeability and tissue homeostasis...
  43. ncbi Isolation and characterisation of a calnexin homologue, clxA, from Aspergillus niger
    H Wang
    Genencor International Inc, Palo Alto, CA 94304, USA
    Mol Genet Genomics 268:684-91. 2003
    ..Deletion studies indicate that clxA is not an essential gene in A. niger...
  44. pmc Association of calnexin with mutant peripheral myelin protein-22 ex vivo: a basis for "gain-of-function" ER diseases
    K M Dickson
    Department of Neurology and Neurosurgery, McGill University, 3640 University Street, Montreal, QC, Canada H3A 2B2
    Proc Natl Acad Sci U S A 99:9852-7. 2002
    ..approximately equal to 11 min), and oligosaccharide processing-dependent manner with the lectin chaperone calnexin (CNX), but not calreticulin nor BiP...
  45. ncbi Localization of neutral ceramidase in caveolin-enriched light membranes of murine endothelial cells
    E Romiti
    Dipartimento di Scienze Biochimiche, Viale G B Morgagni 50, Florence, Italy
    FEBS Lett 506:163-8. 2001
    ..These findings suggest that neutral CDase is implicated, together with SMase activities, in the control of caveolar Cer content that may be critical for caveola dynamics...
  46. pmc Functional expression of the new gap junction gene connexin47 transcribed in mouse brain and spinal cord neurons
    B Teubner
    Institut fur Genetik, Universitat Bonn, D 53117 Bonn, Germany
    J Neurosci 21:1117-26. 2001
    ..Thus, electrical synapses in adult mammalian brain are likely to consist of different connexin proteins depending on the neuronal subtype...
  47. ncbi Syntaxin 3 and Munc-18-2 in epithelial cells during kidney development
    S Lehtonen
    Haartman Institute and Department of Pathology, University of Helsinki, Finland
    Kidney Int 56:815-26. 1999
    ..We have analyzed the expression patterns of vesicle-docking proteins of the syntaxin family in mouse kidney, focusing on syntaxin 3 and its interaction partner, the Sec1-related Munc-18-2...
  48. ncbi Genes regulating MHC class I processing of antigen
    P M van Endert
    INSERM U25 Hôpital Necker, Paris, France
    Curr Opin Immunol 11:82-8. 1999
    ..Finally, genetic evidence suggests an important influence of an unidentified gene, in the MHC complex, on MHC class I processing...
  49. ncbi Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells
    J C Hay
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305 5428, USA
    Cell 89:149-58. 1997
    ..We propose that these protein interactions determine vesicle docking/fusion fidelity between the endoplasmic reticulum and Golgi...
  50. pmc Natural resistance to infection with intracellular pathogens: the Nramp1 protein is recruited to the membrane of the phagosome
    S Gruenheid
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    J Exp Med 185:717-30. 1997
    ....
  51. ncbi Calnexin associates exclusively with individual CD3 delta and T cell antigen receptor (TCR) alpha proteins containing incompletely trimmed glycans that are not assembled into multisubunit TCR complexes
    J E van Leeuwen
    Experimental Immunology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892 1360, USA
    J Biol Chem 271:9660-5. 1996
    ..In addition, these data document that calnexin assembly with CD3 delta and TCR alpha glycoproteins involves both glycan-dependent and glycan-independent mechanisms...
  52. ncbi Molecular cloning and sequencing of calnexin-t. An abundant male germ cell-specific calcium-binding protein of the endoplasmic reticulum
    S Ohsako
    Department of Veterinary Biosciences, University of Illinois, Urbana 61801
    J Biol Chem 269:14140-8. 1994
    ..These results suggest that spermatogenic cell endoplasmic reticulum has a unique calcium binding protein, calnexin-t, which appears to be a calnexin variant...
  53. ncbi Class II histocompatibility molecules associate with calnexin during assembly in the endoplasmic reticulum
    K L Schreiber
    Department of Immunology, Mayo Clinic, Rochester, MN 55905
    Int Immunol 6:101-11. 1994
    ..The isolation of cDNA fragments encoding murine calnexin will allow us to further evaluate the functional consequences of calnexin-class II interaction...
  54. ncbi Localization of three genes expressed in retina on mouse chromosome 11
    C A Kozak
    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
    Mamm Genome 6:142-4. 1995
  55. ncbi Aglycosylated and phosphatidylinositol-anchored MHC class I molecules are associated with calnexin. Evidence implicating the class I-connecting peptide segment in calnexin association
    B M Carreno
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 154:5173-80. 1995
    ..These comparisons of Q7b isoforms implicate the class I-connecting peptide segment and not the transmembrane region as a site of interaction with calnexin...
  56. ncbi Presenilin 1 and presenilin 2 have differential effects on the stability and maturation of nicastrin in Mammalian brain
    Fusheng Chen
    Centre for Research in Neurodegenerative Diseases, Tanz Neuroscience Building, University of Toronto, Ontario M5S 3H2, Canada
    J Biol Chem 278:19974-9. 2003
    ..Our data therefore suggest that the differential interactions of PS1 and PS2 with nicastrin reflect different functions for the PS1 and PS2 complexes...
  57. ncbi Compromised calnexin function in calreticulin-deficient cells
    Rai Knee
    Department of Biochemistry, Canadian Institutes of Health Research Membrane Protein Research Group, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Biochem Biophys Res Commun 304:661-6. 2003
    ..We show that the absence of calreticulin can have devastating effects on the function of the others, compromising overall quality control of the secretory pathway and activating UPR-dependent pathways...
  58. ncbi Development of a deer mouse whole-genome radiation hybrid panel and comparative mapping of Mus chromosome 11 loci
    Clifton M Ramsdell
    Peromyscus Genetic Stock Center, Department of Biological Sciences, University of South Carolina, 700 Sumter Street, Columbia, South Carolina 29208, USA
    Mamm Genome 17:37-48. 2006
    ..Furthermore, this separate linkage group is likely to reside in a chromosomal region of inversion polymorphism between P. maniculatus and P. polionotus...
  59. ncbi Platelet-derived growth factor D induces cardiac fibrosis and proliferation of vascular smooth muscle cells in heart-specific transgenic mice
    Annica Pontén
    Ludwig Institute for Cancer Research, S 17177 Stockholm, Sweden
    Circ Res 97:1036-45. 2005
    ..Our data raise the possibility that this growth factor may be involved in cardiac fibrosis and atherosclerosis...
  60. ncbi Intracellular lipidation of newly synthesized apolipoprotein A-I in primary murine hepatocytes
    Jovana Maric
    Lipoprotein and Atherosclerosis Research Group, University of Ottawa Heart Institute, Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario K1Y 4W7, Canada
    J Biol Chem 280:39942-9. 2005
    ..Kiss, R. S., Franklin, V., Wang, M. D., Haidar, B., and Marcel, Y. L. (2005) J. Biol. Chem. 280, 21612-21621) occurs after secretion at the cell surface...
  61. ncbi Calnexin and ERp57 facilitate the assembly of the neonatal Fc receptor for IgG with beta 2-microglobulin in the endoplasmic reticulum
    Xiaoping Zhu
    Laboratory of Immunology, Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742, USA
    J Immunol 175:967-76. 2005
    ..Mass spectrometry analysis revealed that this band was identical with calnexin (CNX)...
  62. ncbi Soluble tyrosinase is an endoplasmic reticulum (ER)-associated degradation substrate retained in the ER by calreticulin and BiP/GRP78 and not calnexin
    Costin I Popescu
    Institute of Biochemistry, Splaiul Independentei 296, 060031 Bucharest 17, Romania
    J Biol Chem 280:13833-40. 2005
    ..The lack of specificity for calnexin interaction reveals a novel role for calreticulin in OCAI albinism...
  63. pmc Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis
    Kazuhide Watanabe
    Department of Vascular Biology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan
    J Clin Invest 114:898-907. 2004
    ..We propose vasohibin to be an endothelium-derived negative feedback regulator of angiogenesis...
  64. pmc Presenilin 1 mediates the turnover of telencephalin in hippocampal neurons via an autophagic degradative pathway
    Cary Esselens
    Membrane Trafficking Laboratory, CME VIB04, Gasthuisberg KU Leuven, 3000 Leuven, Belgium
    J Cell Biol 166:1041-54. 2004
    ..Collectively, PS1 deficiency affects in a gamma-secretase-independent fashion the turnover of TLN through autophagic vacuoles, most likely by an impaired capability to fuse with lysosomes...
  65. pmc MARCKS-like protein, a membrane protein identified for its expression in developing neural retina, plays a role in regulating retinal cell proliferation
    Jing Zhao
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, 108 8639, Tokyo, Japan
    Biochem J 408:51-9. 2007
    ..Mutation analysis of MLP demonstrated that myristoylation was necessary to promote proliferation and that phosphorylation inhibited proliferation, indicating the functional importance of membrane localization...
  66. ncbi Both raft- and non-raft proteins associate with CHAPS-insoluble complexes: some APP in large complexes
    Alexander Rouvinski
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    Biochem Biophys Res Commun 308:750-8. 2003
    ..Large CHAPS-insoluble complexes resemble, but are not equal to, classical rafts. These findings extend considerably the range of detergent-insoluble membranal domains...
  67. pmc Role of calnexin in the glycan-independent quality control of proteolipid protein
    Eileithyia Swanton
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    EMBO J 22:2948-58. 2003
    ....
  68. pmc The molecular chaperone calnexin is expressed on the surface of immature thymocytes in association with clonotype-independent CD3 complexes
    D L Wiest
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 14:3425-33. 1995
    ....
  69. ncbi A novel cardiolipin-remodeling pathway revealed by a gene encoding an endoplasmic reticulum-associated acyl-CoA:lysocardiolipin acyltransferase (ALCAT1) in mouse
    Jingsong Cao
    Endocrine Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    J Biol Chem 279:31727-34. 2004
    ..ALCAT1 represents the first identified cardiolipin-remodeling enzyme from any living organism; its identification implies a novel role for the endoplasmic reticulum in cardiolipin metabolism...
  70. ncbi Endoplasmic reticulum stress during the embryonic development of the central nervous system in the mouse
    Xiaochu Zhang
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Int J Dev Neurosci 25:455-63. 2007
    ..Furthermore, our data suggest that ER stress-like mechanism may induce apoptosis via activation of the caspases during embryonic development of the central nervous system...
  71. ncbi Retention of unassembled components of integral membrane proteins by calnexin
    S Rajagopalan
    Department of Rheumatology and Immunology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115
    Science 263:387-90. 1994
    ..Since engineered calnexin determined the intracellular localization of the proteins associated with it, it is concluded that calnexin interacts with incompletely assembled TCR components and retains them in the ER...
  72. ncbi A predominant role of acyl-CoA:monoacylglycerol acyltransferase-2 in dietary fat absorption implicated by tissue distribution, subcellular localization, and up-regulation by high fat diet
    Jingsong Cao
    Division of Endocrine Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    J Biol Chem 279:18878-86. 2004
    ..Taken together, our data suggest a predominant role of MGAT2 in dietary fat absorption...

Research Grants30

  1. Cancer cell adaptation to metabolic stress
    Craig Thompson; Fiscal Year: 2007
    ..Ultimately we hope this information may be used to design novel strategies to specifically treat transformed tumor cells growing under conditions of nutrient and oxygen limitation. ..
  2. Apoptosis Control by Bcl-2 Family Members
    Craig Thompson; Fiscal Year: 2009
    ....
  3. Lymphocyte Survival: Implications for lymphomagenesis
    Craig Thompson; Fiscal Year: 2009
    ....
  4. Apoptosis Control by Bcl-2 Family Members
    Craig B Thompson; Fiscal Year: 2010
    ....
  5. Lymphocyte Survival: Implications for lymphomagenesis
    Craig B Thompson; Fiscal Year: 2010
    ....
  6. Cancer cell survival during factor deprivation
    Craig B Thompson; Fiscal Year: 2010
    ..This project seeks to determine whether oncogenic reprogramming of metabolism can be exploited to develop new cancer therapeutic strategies. ..
  7. Cancer cell survival during factor deprivation
    Craig Thompson; Fiscal Year: 2007
    ..Such knowledge may ultimately help us design novel treatment strategies that exploit the metabolic abnormalities of tumor cells carrying alterations in these two antiapoptotic pathways. ..
  8. Apoptosis Control by Proapoptotic Bcl-2 Family Members
    Craig Thompson; Fiscal Year: 2007
    ..abstract_text> ..
  9. Apoptosis Control by Proapoptotic Bcl-2 Family Members
    Craig Thompson; Fiscal Year: 2003
    ..Through defining the molecular mechanisms by which Bcl-2 proteins contribute to the regulation of cell survival and death, we hope to contribute information that will lead to the development of new cancer treatments. ..
  10. Lymphocyte Survival--Implications for Lymphomagenesis
    Craig Thompson; Fiscal Year: 2005
    ....
  11. Apoptosis Control by Proapoptotic Bcl-2 Family Members
    Craig Thompson; Fiscal Year: 2006
    ..Through defining the molecular mechanisms by which Bcl-2 proteins contribute to the regulation of cell survival and death, we hope to contribute information that will lead to the development of new cancer treatments. ..
  12. Metabolomics: From Bioenergetics To Apoptosis
    Craig Thompson; Fiscal Year: 2005
    ..Understanding the relationships between metabolism and apoptosis may lead to novel approaches for the prevention and/or treatment of cancer and degenerative disorders. ..
  13. Lymphocyte Survival: Implications for lymphomagenesis
    Craig Thompson; Fiscal Year: 2009
    ....