Gene Symbol: Asap1
Description: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Alias: AV239055, DEF-1, Ddef1, PAP, mKIAA1249, s19, arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, PIP2-dependent ARF1 GAP, development and differentiation-enhancing factor 1
Species: mouse
Products:     Asap1

Top Publications

  1. Brown M, Andrade J, Radhakrishna H, Donaldson J, Cooper J, Randazzo P. ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src. Mol Cell Biol. 1998;18:7038-51 pubmed
    ..The cDNA for ASAP1 was independently identified in a screen for proteins that interact with the SH3 domain of the tyrosine kinase Src...
  2. Kruljac Letunic A, Moelleken J, Kallin A, Wieland F, Blaukat A. The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1. J Biol Chem. 2003;278:29560-70 pubmed
    ..A prominent binder of Pyk2 identified by this method was the Arf-GTPase-activating protein ASAP1. Pyk2-ASAP1 interaction was confirmed in pull-down as well as in co-immunoprecipitation experiments, and contact ..
  3. Kam J, Miura K, Jackson T, Gruschus J, Roller P, Stauffer S, et al. Phosphoinositide-dependent activation of the ADP-ribosylation factor GTPase-activating protein ASAP1. Evidence for the pleckstrin homology domain functioning as an allosteric site. J Biol Chem. 2000;275:9653-63 pubmed
    ..the activities are coordinated, we have examined the role of phosphoinositide binding for Arf GAP function using ASAP1/centaurin beta4 as a model...
  4. Jain S, Yoon S, Zhu L, Brodbeck J, Dai J, Walker D, et al. Arf4 determines dentate gyrus-mediated pattern separation by regulating dendritic spine development. PLoS ONE. 2012;7:e46340 pubmed publisher
    ..Arf4's effects on spine development are regulated by ASAP1, a GTPase-activating protein that modulates Arf4 GTPase activity...
  5. Klein S, Franco M, Chardin P, Luton F. AlFx affects the formation of focal complexes by stabilizing the Arf-GAP ASAP1 in a complex with Arf1. FEBS Lett. 2005;579:5741-5 pubmed
    ..demonstrate that Arf1GDP can be converted into an active conformation by AlFx to form a complex with the Arf-GAP ASAP1 in vitro and in vivo...
  6. Uslu V, Petretich M, Ruf S, Langenfeld K, Fonseca N, Marioni J, et al. Long-range enhancers regulating Myc expression are required for normal facial morphogenesis. Nat Genet. 2014;46:753-8 pubmed publisher
    ..This dual molecular etiology may account for the prominent influence of variants in the 8q24 region on human facial dysmorphologies. ..
  7. Abramova N, Charniga C, Goderie S, Temple S. Stage-specific changes in gene expression in acutely isolated mouse CNS progenitor cells. Dev Biol. 2005;283:269-81 pubmed
    ..These data indicate that progenitor cells change during development and that adult and embryonic neural progenitor cells are intrinsically different. ..
  8. Yamabhai M, Kay B. Examining the specificity of Src homology 3 domain--ligand interactions with alkaline phosphatase fusion proteins. Anal Biochem. 1997;247:143-51 pubmed
  9. Chen P, Jian X, Heissler S, Le K, Luo R, Jenkins L, et al. The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network. J Biol Chem. 2016;291:7517-26 pubmed publisher
    b>ASAP1 regulates F-actin-based structures and functions, including focal adhesions (FAs) and circular dorsal ruffles (CDRs), cell spreading and migration. ASAP1 function requires its N-terminal BAR domain...

More Information


  1. Liu Y, Yerushalmi G, Grigera P, Parsons J. Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling. J Biol Chem. 2005;280:8884-92 pubmed
    ..b>ASAP1 (Arf-GAP containing SH3, ankyrin repeats, and PH domain) is a phospholipid-dependent Arf GTPase-activating protein ..
  2. Bharti S, Inoue H, Bharti K, Hirsch D, Nie Z, Yoon H, et al. Src-dependent phosphorylation of ASAP1 regulates podosomes. Mol Cell Biol. 2007;27:8271-83 pubmed
    Invadopodia are Src-induced cellular structures that are thought to mediate tumor invasion. ASAP1, an Arf GTPase-activating protein (GAP) containing Src homology 3 (SH3) and Bin, amphiphysin, and RVS161/167 (BAR) domains, is a substrate ..
  3. Shiba Y, Randazzo P. GEFH1 binds ASAP1 and regulates podosome formation. Biochem Biophys Res Commun. 2011;406:574-9 pubmed publisher
    Invadopodia are cellular structures that are thought to mediate tumor invasion. ASAP1, an Arf GTPase-activating protein (GAP) containing a BAR domain, is a substrate of Src...
  4. Wang J, Morita Y, Mazelova J, Deretic D. The Arf GAP ASAP1 provides a platform to regulate Arf4- and Rab11-Rab8-mediated ciliary receptor targeting. EMBO J. 2012;31:4057-71 pubmed publisher
    ..represented by rhodopsin, is mediated by a specialized system, the principal component of which is the Arf GAP ASAP1. Ablation of ASAP1 abolishes ciliary targeting and causes formation of actin-rich periciliary membrane projections ..
  5. Nie Z, Hirsch D, Luo R, Jian X, Stauffer S, Cremesti A, et al. A BAR domain in the N terminus of the Arf GAP ASAP1 affects membrane structure and trafficking of epidermal growth factor receptor. Curr Biol. 2006;16:130-9 pubmed
    ..We have examined the role of a putative BAR domain in the function of the Arf GAP ASAP1. ASAP1's N terminus, containing the putative BAR domain together with a PH domain, dimerized to form an extended ..
  6. Haque A, Noman A, Koide N, Odkhuu E, Naiki Y, Hashimoto S, et al. An ADP ribosylation factor-GTPase activating protein negatively regulates the production of proinflammatory mediators in response to lipopolysaccharide. Cancer Immunol Immunother. 2011;60:1439-46 pubmed publisher
    An ADP ribosylation factor-GTPase activating protein (ASAP1) is highly expressed in a variety of tumor cells and is involved in the cell motility, invasion, and metastasis...
  7. Oshiro T, Koyama S, Sugiyama S, Kondo A, Onodera Y, Asahara T, et al. Interaction of POB1, a downstream molecule of small G protein Ral, with PAG2, a paxillin-binding protein, is involved in cell migration. J Biol Chem. 2002;277:38618-26 pubmed
    ..we screened for POB1-binding proteins using a yeast two-hybrid method and found that POB1 interacts with mouse ASAP1, which is a human PAG2 homolog...
  8. Liu Y, Loijens J, Martin K, Karginov A, Parsons J. The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly. Mol Biol Cell. 2002;13:2147-56 pubmed
    b>ASAP1 (ADP ribosylation factor [ARF]- GTPase-activating protein [GAP] containing SH3, ANK repeats, and PH domain) is a phospholipid-dependent ARF-GAP that binds to and is phosphorylated by pp60(Src)...
  9. Ha V, Bharti S, Inoue H, Vass W, Campa F, Nie Z, et al. ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion. J Biol Chem. 2008;283:14915-26 pubmed publisher
    ..We have examined in vitro and in vivo functions of ASAP3 and compared it to the related Arf GAP ASAP1 that has also been implicated in oncogenesis...
  10. Furman C, Short S, Subramanian R, Zetter B, Roberts T. DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002;277:7962-9 pubmed
    DEF-1/ASAP1 is an ADP-ribosylation factor GTPase-activating protein (ARF GAP) that localizes to focal adhesions and is involved in cytoskeletal regulation...
  11. King F, Hu E, Harris D, Sarraf P, Spiegelman B, Roberts T. DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines. Mol Cell Biol. 1999;19:2330-7 pubmed
    ..These results suggest that DEF-1 is an important component of a signal transduction process that is involved in the differentiation of fibroblasts and possibly of other types of cells. ..
  12. Sheridan J, Ritchie M, Best S, Jiang K, Beck T, Vaillant F, et al. A pooled shRNA screen for regulators of primary mammary stem and progenitor cells identifies roles for Asap1 and Prox1. BMC Cancer. 2015;15:221 pubmed publisher
    ..Two genes, Asap1 and Prox1, respectively implicated in breast cancer metastasis and progenitor cell function in other systems, were ..