Gene Symbol: Art2b
Description: ADP-ribosyltransferase 2b
Alias: ART2.2, ARTC2, Art, Ly92b, Rt-6, Rt6, Rt6-2, T-cell ecto-ADP-ribosyltransferase 2, ADP-ribosyltransferase C2 and C3 toxin-like 2, NAD(+) glycohydrolase, T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2, T-cell differentiation marker Rt6 homolog 2, T-cell mono(ADP-ribosyl)transferase 2, mono(ADP-ribosyl)transferase 2B
Species: mouse
Products:     Art2b

Top Publications

  1. Krebs C, Adriouch S, Braasch F, Koestner W, Leiter E, Seman M, et al. CD38 controls ADP-ribosyltransferase-2-catalyzed ADP-ribosylation of T cell surface proteins. J Immunol. 2005;174:3298-305 pubmed
  2. Adriouch S, Hubert S, Pechberty S, Koch Nolte F, Haag F, Seman M. NAD+ released during inflammation participates in T cell homeostasis by inducing ART2-mediated death of naive T cells in vivo. J Immunol. 2007;179:186-94 pubmed
    ..We propose that NAD(+) released during early inflammation facilitates the expansion of primed T cells, through ART2-driven death of resting cells, thus contributing to the dynamic regulation of T cell homeostasis. ..
  3. Prochazka M, Gaskins H, Leiter E, Koch Nolte F, Haag F, Thiele H. Chromosomal localization, DNA polymorphism, and expression of Rt-6, the mouse homologue of rat T-lymphocyte differentiation marker RT6. Immunogenetics. 1991;33:152-6 pubmed
  4. Hara N, Terashima M, Shimoyama M, Tsuchiya M. Mouse T-cell antigen rt6.1 has thiol-dependent NAD glycohydrolase activity. J Biochem. 2000;128:601-7 pubmed
    Mouse Rt6.1 and Rt6.2, homologues of rat T-cell RT6 antigens, catalyze arginine-specific ADP-ribosylation. Without an added ADP-ribose acceptor, Rt6.2 shows NAD glycohydrolase (NADase) activity. However, Rt6...
  5. Braren R, Glowacki G, Nissen M, Haag F, Koch Nolte F. Molecular characterization and expression of the gene for mouse NAD+:arginine ecto-mono(ADP-ribosyl)transferase, Art1. Biochem J. 1998;336 ( Pt 3):561-8 pubmed
    ..The availability of the Art1 gene provides the basis for applying transgene and knockout technologies to further probe the function of this gene product. ..
  6. Hollmann C, Haag F, Schlott M, Damaske A, Bertuleit H, Matthes M, et al. Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: cloning of a second functional gene and identification of the Rt6 gene products. Mol Immunol. 1996;33:807-17 pubmed
    b>RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen...
  7. Glowacki G, Braren R, Cetkovic Cvrlje M, Leiter E, Haag F, Koch Nolte F. Structure, chromosomal localization, and expression of the gene for mouse ecto-mono(ADP-ribosyl)transferase ART5. Gene. 2001;275:267-77 pubmed
    ..6 cM in close proximity to the Art1, Art2a and Art2b genes...
  8. Chen Y, Scheuplein F, Driver J, Hewes A, Reifsnyder P, Leiter E, et al. Testing the role of P2X7 receptors in the development of type 1 diabetes in nonobese diabetic mice. J Immunol. 2011;186:4278-84 pubmed publisher
    ..This study demonstrated the presence of a T1D resistance gene closely linked to the ablated Cd38 allele in the previously reported NOD stock also lacking ART2, but not in the newly generated CD38/P2X(7) double-KO line. ..
  9. Kawamura H, Aswad F, Minagawa M, Govindarajan S, Dennert G. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol. 2006;176:2152-60 pubmed
    ..These results prompt the conclusion that engagement of P2X(7)Rs on NKT cells inhibits naive, while stimulating activated cells, resulting in suppression or stimulation of autoimmune hepatitis. ..

More Information


  1. Chen J, Chen Y, Reifsnyder P, Schott W, Lee C, Osborne M, et al. Targeted disruption of CD38 accelerates autoimmune diabetes in NOD/Lt mice by enhancing autoimmunity in an ADP-ribosyltransferase 2-dependent fashion. J Immunol. 2006;176:4590-9 pubmed
    ..Unexpectedly, diabetes development in the combined CD38/ART2 stock was strongly suppressed, possibly through epistatic interactions between genes linked to the targeted CD38 on Chromosome 5 and the ART2 complex on Chromosome 7. ..
  2. Chen Y, Chen J, Osborne M, Chapman H, Besra G, Porcelli S, et al. CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice. J Immunol. 2006;177:2939-47 pubmed
    ..Therefore, this study documents a previously unrecognized role for CD38 in maintaining survival of an iNKT cell subset that preferentially contributes to the maintenance of immunological tolerance. ..
  3. Ohlrogge W, Haag F, Löhler J, Seman M, Littman D, Killeen N, et al. Generation and characterization of ecto-ADP-ribosyltransferase ART2.1/ART2.2-deficient mice. Mol Cell Biol. 2002;22:7535-42 pubmed
    ..showed a dramatically reduced capacity to ADP-ribosylate cell surface proteins, indicating that most if not all ART activity on the T-cell surface can be attributed to the ART2s...
  4. Bannas P, Adriouch S, Kahl S, Braasch F, Haag F, Koch Nolte F. Activity and specificity of toxin-related mouse T cell ecto-ADP-ribosyltransferase ART2.2 depends on its association with lipid rafts. Blood. 2005;105:3663-70 pubmed
    ..Our results further suggest that raft association focuses ART2.2 on specific targets that constitutively or inducibly associate with lipid rafts. ..
  5. Koch Nolte F, Kuhl M, Haag F, Cetkovic Cvrlje M, Leiter E, Thiele H. Assignment of the human and mouse genes for muscle ecto mono (ADPribosyl)transferase to a conserved linkage group on human chromosome 11p15 and mouse chromosome 7. Genomics. 1996;36:215-6 pubmed
  6. Bannas P, Scheuplein F, Well L, Hermans Borgmeyer I, Haag F, Koch Nolte F. Transgenic overexpression of toxin-related ecto-ADP-ribosyltransferase ART2.2 sensitizes T cells but not B cells to NAD-induced cell death. Mol Immunol. 2011;48:1762-70 pubmed publisher
    ..The ART2.2-overexpressing animals described here confirm that ART2.2 is an essential component for the regulation of T-cell functions by extracellular NAD and provide a useful tool to further elucidate the function of ART2.2 in vivo. ..
  7. Koch Nolte F, Petersen D, Balasubramanian S, Haag F, Kahlke D, Willer T, et al. Mouse T cell membrane proteins Rt6-1 and Rt6-2 are arginine/protein mono(ADPribosyl)transferases and share secondary structure motifs with ADP-ribosylating bacterial toxins. J Biol Chem. 1996;271:7686-93 pubmed exhibit significant sequence similarity only to one other known protein, the T cell differentiation antigen Rt6. In this paper we describe secondary structure analyses of Rt6 and related proteins and show conserved structure ..
  8. Kanaitsuka T, Bortell R, Stevens L, Moss J, Sardinha D, Rajan T, et al. Expression in BALB/c and C57BL/6 mice of Rt6-1 and Rt6-2 ADP-ribosyltransferases that differ in enzymatic activity: C57BL/6 Rt6-1 is a natural transferase knockout. J Immunol. 1997;159:2741-9 pubmed
    ..Rat T cells that express the ART designated RT6 are determinants of the expression of autoimmune diabetes...
  9. Glowacki G, Braren R, Firner K, Nissen M, Kühl M, Reche P, et al. The family of toxin-related ecto-ADP-ribosyltransferases in humans and the mouse. Protein Sci. 2002;11:1657-70 pubmed
    ..The human and mouse ART genes map to chromosomal regions with conserved linkage synteny...
  10. Lischke T, Schumacher V, Wesolowski J, Hurwitz R, Haag F, Koch Nolte F, et al. CD8-? ADP-ribosylation affects CD8(+) T-cell function. Eur J Immunol. 2013;43:1828-38 pubmed publisher
    ..We propose that ADP-ribosylation of CD8-? can regulate the coreceptor function of CD8 in the presence of elevated levels of extracellular NAD(+) . ..
  11. Koch Nolte F, Klein J, Hollmann C, Kuhl M, Haag F, Gaskins H, et al. Defects in the structure and expression of the genes for the T cell marker Rt6 in NZW and (NZB x NZW)F1 mice. Int Immunol. 1995;7:883-90 pubmed
    b>Rt6 is a T cell-restricted GPI-anchored membrane protein and a member of the family of mono(ADP-ribosyl)transferases. One of the two murine Rt6 genes is deleted in NZW mice...
  12. Sardinha D, Rajan T. Cis-acting regulation of splenic Art2 gene expression in inbred mouse strains. Immunogenetics. 1999;49:700-3 pubmed
  13. Hara N, Badruzzaman M, Sugae T, Shimoyama M, Tsuchiya M. Mouse Rt6.1 is a thiol-dependent arginine-specific ADP-ribosyltransferase. Eur J Biochem. 1999;259:289-94 pubmed
    Mouse T-cell antigens Rt6.1 and Rt6.2 are glycosylphosphatidylinositol-anchored arginine-specific adenosine diphosphate (ADP)-ribosyltransferases...
  14. Heiss K, Jänner N, Mähnss B, Schumacher V, Koch Nolte F, Haag F, et al. High sensitivity of intestinal CD8+ T cells to nucleotides indicates P2X7 as a regulator for intestinal T cell responses. J Immunol. 2008;181:3861-9 pubmed
    ..Overall, our observations define P2X7 as a new regulatory element in the control of CD8(+) T cell responses in the intestinal mucosa. ..
  15. Hong S, Schwarz N, Brass A, Seman M, Haag F, Koch Nolte F, et al. Differential regulation of P2X7 receptor activation by extracellular nicotinamide adenine dinucleotide and ecto-ADP-ribosyltransferases in murine macrophages and T cells. J Immunol. 2009;183:578-92 pubmed publisher
    ..This modification is catalyzed by ART2.2, a GPI-anchored ADP-ribosyltransferase (ART) that is constitutively expressed in murine T cells. We previously reported that ART2...
  16. Hubert S, Rissiek B, Klages K, Huehn J, Sparwasser T, Haag F, et al. Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. J Exp Med. 2010;207:2561-8 pubmed publisher
    ..Collectively, our data demonstrate that NAD(+) influences survival, phenotype, and function of T reg cells and provide proof of principle that acting on the ART2-P2X7 pathway represents a new strategy to manipulate T reg cells in vivo. ..
  17. Chen J, Reifsnyder P, Scheuplein F, Schott W, Mileikovsky M, Soodeen Karamath S, et al. "Agouti NOD": identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells. Mamm Genome. 2005;16:775-83 pubmed
    ..CBALs- Tyr(+)/Lt blastocysts produced approximately 50% live-born mice, of which approximately 11% were chimeric. Presumably because of high genomic instability, no germline transmission was observed. ..
  18. Menzel S, Rissiek B, Bannas P, Jakoby T, Miksiewicz M, Schwarz N, et al. Nucleotide-Induced Membrane-Proximal Proteolysis Controls the Substrate Specificity of T Cell Ecto-ADP-Ribosyltransferase ARTC2.2. J Immunol. 2015;195:2057-66 pubmed publisher
    b>ARTC2.2 is a toxin-related, GPI-anchored ADP-ribosyltransferase expressed by murine T cells. In response to NAD(+) released from damaged cells during inflammation, ARTC2.2 ADP-ribosylates and thereby gates the P2X7 ion channel...